AlfalfaGEDB | MS.gene23229

M. sativa cultivar XinJiangDaYe / MS.gene23229

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23229.t1	AES78884.2	87.2	297	37	1	1	296	1	297	1.30E-145	525.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23229.t1	Q8L3W1	27.7	328	174	8	2	279	22	336	2.4e-23	110.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23229.t1	G7KVV7	87.2	297	37	1	1	296	1	297	9.4e-146	525.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene23229.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23229.t1	MTR_7g050590	87.205	297	37	1	1	296	1	297	0.0	527
MS.gene23229.t1	MTR_7g050560	75.085	293	55	4	1	292	28	303	2.66e-156	439
MS.gene23229.t1	MTR_7g050710	69.565	299	84	5	2	296	29	324	1.43e-148	419
MS.gene23229.t1	MTR_3g098790	70.707	297	78	4	1	296	28	316	8.42e-146	412
MS.gene23229.t1	MTR_7g050580	70.548	292	70	3	1	292	28	303	2.07e-145	411
MS.gene23229.t1	MTR_7g050720	62.712	295	94	8	1	281	25	317	1.11e-120	348
MS.gene23229.t1	MTR_1g035460	36.755	302	153	8	1	279	1	287	1.56e-52	177
MS.gene23229.t1	MTR_1g108780	36.949	295	152	11	2	279	13	290	7.93e-51	174
MS.gene23229.t1	MTR_8g103940	35.836	293	157	9	2	279	55	331	1.69e-46	163
MS.gene23229.t1	MTR_7g050640	69.767	86	26	0	1	86	28	113	7.94e-37	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23229.t1	AT3G18990	28.097	331	175	10	2	282	22	339	5.35e-30	117
MS.gene23229.t1	AT3G18990	28.049	328	173	10	2	279	22	336	7.97e-30	115
MS.gene23229.t1	AT4G01580	45.455	88	48	0	2	89	46	133	2.43e-19	84.3
MS.gene23229.t1	AT4G33280	27.063	303	190	11	1	286	38	326	2.07e-18	84.3
MS.gene23229.t1	AT1G49475	44.186	86	47	1	2	87	50	134	2.30e-18	81.6
MS.gene23229.t1	AT3G18960	44.318	88	49	0	2	89	46	133	3.88e-18	80.9
MS.gene23229.t1	AT3G18960	44.318	88	49	0	2	89	46	133	4.84e-18	81.3
MS.gene23229.t1	AT3G18960	44.318	88	49	0	2	89	9	96	6.34e-18	80.1

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Find 48 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTAATAGGGTCTCCAA+TGG	0.643575	7.2:-52504100	MS.gene23229:CDS
AAATATGGTTTGAAAAGGGT+TGG	0.440218	7.2:-52504261	MS.gene23229:CDS
AAGGACCACAACTTGAAAGT+TGG	0.549394	7.2:-52502971	MS.gene23229:CDS
AATAGGGTCTCCAATGGTCC+AGG	0.542970	7.2:-52504094	MS.gene23229:CDS
AATTTCCACTTTGCACCATT+TGG	0.355722	7.2:+52504307	None:intergenic
AATTTGAAATCACCGGTGGA+TGG	0.596243	7.2:-52503006	MS.gene23229:CDS
ACGTGGTTGATGAAATTCAA+AGG	0.544951	7.2:+52504015	None:intergenic
AGAAAGTTTGTAGAGAAATA+TGG	0.315950	7.2:-52504364	MS.gene23229:CDS
AGACTCCAAATGGTGCAAAG+TGG	0.616755	7.2:-52504312	MS.gene23229:CDS
AGGTGTTAAATAAAGGAGTT+TGG	0.387066	7.2:-52503069	MS.gene23229:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATATGGTTTGAAAAGGGT+TGG	-	chr7.2:52502965-52502984	MS.gene23229:CDS	30.0%
AAGACACAATCTTATACCTT+TGG	+	chr7.2:52503313-52503332	None:intergenic	30.0%
AAGTATTTGAATAACGCTTG+AGG	-	chr7.2:52503766-52503785	MS.gene23229:CDS	30.0%
AGGTGTTAAATAAAGGAGTT+TGG	-	chr7.2:52504157-52504176	MS.gene23229:CDS	30.0%
AGTATTTGAATAACGCTTGA+GGG	-	chr7.2:52503767-52503786	MS.gene23229:CDS	30.0%
AGTTGCAGCAAAAACTAATA+GGG	-	chr7.2:52503116-52503135	MS.gene23229:CDS	30.0%
ATACATCTAAAGACTCCAAA+TGG	-	chr7.2:52502904-52502923	MS.gene23229:CDS	30.0%
CTCTACTCTTTACTAGATTA+AGG	-	chr7.2:52503590-52503609	MS.gene23229:intron	30.0%
GAAAGGGTAATTTATCTCTA+AGG	+	chr7.2:52503091-52503110	None:intergenic	30.0%
GATATTTGAAAAGCAGAAGA+TGG	+	chr7.2:52503026-52503045	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	52502853	52504395	52502853	ID=MS.gene23229
chr7.2	mRNA	52502853	52504395	52502853	ID=MS.gene23229.t1;Parent=MS.gene23229
chr7.2	exon	52503933	52504395	52503933	ID=MS.gene23229.t1.exon1;Parent=MS.gene23229.t1
chr7.2	CDS	52503933	52504395	52503933	ID=cds.MS.gene23229.t1;Parent=MS.gene23229.t1
chr7.2	exon	52503708	52503829	52503708	ID=MS.gene23229.t1.exon2;Parent=MS.gene23229.t1
chr7.2	CDS	52503708	52503829	52503708	ID=cds.MS.gene23229.t1;Parent=MS.gene23229.t1
chr7.2	exon	52502853	52503158	52502853	ID=MS.gene23229.t1.exon3;Parent=MS.gene23229.t1
chr7.2	CDS	52502853	52503158	52502853	ID=cds.MS.gene23229.t1;Parent=MS.gene23229.t1

Gene Sequence

>MS.gene23229

ATGATGCCAAGAAAGTTTGTAGAGAAATATGGAGAAGGTTTACCAAAAGATATACATCTAAAGACTCCAAATGGTGCAAAGTGGAAATTAAATTTGGTAAAAAGTGATGGCAAAATATGGTTTGAAAAGGGTTGGAAAGAATTTGCAGAGTATCACTCTCTAGCTCATGGCCATCTTCTGCTTTTCAAATATCAAAGAAATACTCATTTTCTTGTGCACATCTTTGAAAAGAGTGCCTTAGAGATAAATTACCCTTTCCAAAGAGTTGCAGCAAAAACTAATAGGGTCTCCAATGGTCCAGGAAATAAACCTCCAAGTCATGAACATTGTAGAGCATCTCAAAAGAGAAAGGTTAACTCCTTTGAATTTCATCAACCACGTGAAATTGGAAGCAGTAGTTGTTTTAGAGTTGGTAAACTGCAGAAGGTAGCATCATTGCATCATACTGACAGAGAGTCCAAAGGTATAAGATTGTGTCTTCATTGTGTCATCAAAGTTTCAGAAGTGAACATGAAAGATGATCATGATTCATGAAATATTATCCTTATTGTGTTCCAATTTTGCAGGGAAAGAAGTGATTACTGCTAAGAGAGTTACAGCTCTTGAAAGAGCACAATCTTTCAAAACATGTAATCCATCCTTTGTTGTTGTCATGCGTGCATCATACGTTGATCGTCATTTTCTACTGGTAAGTTACAATGAAATATATGGCTTGTAGAATCAATAACCAAATCTTTCTCTACTCTTTACTAGATTAAGGCGTGAGAAATATATATTGACTATCTAAAAATAGTTCAACTATGTATGCACTAAGGATAATTAGAATATAATCAAGAGTTGTTAGAGGAGTTACTTAGTTGATTAGTTTGTTAGGTGATTGATAGGTTCTGTTGTTATTTTGTTTATTCTCGACAAGTATTTGAATAACGCTTGAGGGAAGGGGATAATTTTGATTAAACGAGTTTTGTGACTGATTAATACATATTTTCGATCTTGATTAATACATGTTTTCTGTTTTCTTTCTCTTTTCATCTTTCCGTTGATACCAACTGTTCCCTGTTGAGCAATTGTGCCCAGCTCAAATGGACAATCCTAACAAGTGACATCAAGATTAGATAAACGTACAAAAACACTGCTAATGCATAGTTCTTCACATTAAATCGTTTAAAAAATCGTACTATCAACAAGAACTTTCAATTTTTTATTCATTAACATTGTCTTTTCTTTTAATTGGCAGAATATACCACGTATGTTTGGTAATAGACACTTTGATTTGGATAAGAAGAGAGGAGATATTTATTTTCAGGTGTTAAATAAAGGAGTTTGGCCTGAAAAGTACTCTATCAGGAAGACCCGTAACGGACTACAATTTGAAATCACCGGTGGATGGAAGGCATTTGTAAAGGACCACAACTTGAAAGTTGGCGATGTTTGCAAATTTGAACTCATCTCTAGCACTATACTTACCTTCATTGTTCATATCTTTAGAGATACAGACAATGACAGCACAAAGTGTTCAACTTCTCAAAGTAGAATCAATTGA

Protein sequence

>MS.gene23229.t1

MMPRKFVEKYGEGLPKDIHLKTPNGAKWKLNLVKSDGKIWFEKGWKEFAEYHSLAHGHLLLFKYQRNTHFLVHIFEKSALEINYPFQRVAAKTNRVSNGPGNKPPSHEHCRASQKRKVNSFEFHQPREIGSSSCFRVGKLQKVASLHHTDRESKGKEVITAKRVTALERAQSFKTCNPSFVVVMRASYVDRHFLLNIPRMFGNRHFDLDKKRGDIYFQVLNKGVWPEKYSIRKTRNGLQFEITGGWKAFVKDHNLKVGDVCKFELISSTILTFIVHIFRDTDNDSTKCSTSQSRIN