AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23230.t1 MTR_7g050560 82.699 289 48 2 1 288 28 315 5.96e-180 498
MS.gene23230.t1 MTR_7g050590 72.789 294 61 3 1 276 1 293 1.16e-155 436
MS.gene23230.t1 MTR_7g050580 66.997 303 70 4 1 288 28 315 2.96e-140 398
MS.gene23230.t1 MTR_3g098790 66.096 292 76 4 1 276 28 312 1.49e-134 384
MS.gene23230.t1 MTR_7g050710 65.870 293 81 4 2 276 29 320 3.02e-133 380
MS.gene23230.t1 MTR_7g050720 60.410 293 88 6 1 265 25 317 1.46e-116 338
MS.gene23230.t1 MTR_1g035460 37.458 299 139 8 1 263 1 287 1.31e-53 179
MS.gene23230.t1 MTR_1g108780 35.959 292 143 10 2 263 13 290 1.24e-50 173
MS.gene23230.t1 MTR_8g103940 37.370 289 142 9 2 263 55 331 3.73e-47 164
MS.gene23230.t1 MTR_3g061320 36.331 278 136 9 2 262 26 279 6.84e-42 145
MS.gene23230.t1 MTR_5g044670 30.241 291 173 8 1 271 31 311 5.56e-37 134
MS.gene23230.t1 MTR_7g050640 68.605 86 27 0 1 86 28 113 7.60e-37 127
MS.gene23230.t1 MTR_3g061350 32.601 273 158 9 2 262 26 284 1.05e-35 129
MS.gene23230.t1 MTR_3g061370 32.979 282 145 9 2 262 23 281 1.58e-35 129
MS.gene23230.t1 MTR_3g061340 32.963 270 151 8 2 262 26 274 1.36e-34 126
MS.gene23230.t1 MTR_3g061310 31.636 275 159 9 2 262 26 285 3.98e-32 120
MS.gene23230.t1 MTR_1g035570 32.000 275 166 7 2 263 21 287 4.57e-30 117
MS.gene23230.t1 MTR_1g035640 31.338 284 165 10 2 271 21 288 1.03e-27 111
MS.gene23230.t1 MTR_1g035620 28.077 260 180 5 2 261 25 277 2.31e-26 107
MS.gene23230.t1 MTR_3g005490 26.642 274 144 7 2 266 37 262 1.14e-25 102
MS.gene23230.t1 MTR_3g061330 54.217 83 38 0 2 84 26 108 1.29e-25 102
MS.gene23230.t1 MTR_1g021360 28.137 263 172 5 2 250 198 457 2.24e-25 105
MS.gene23230.t1 MTR_3g005480 27.612 268 150 8 2 266 37 263 4.64e-25 101
MS.gene23230.t1 MTR_4g119990 28.676 272 165 7 2 249 31 297 1.08e-24 102
MS.gene23230.t1 MTR_3g061290 54.217 83 38 0 2 84 26 108 1.44e-24 98.2
MS.gene23230.t1 MTR_6g043890 28.676 272 159 9 2 249 31 291 1.52e-24 101
MS.gene23230.t1 MTR_1g050845 27.341 267 161 4 2 261 25 265 7.40e-24 101
MS.gene23230.t1 MTR_5g070440 26.316 266 164 6 2 266 37 271 1.01e-23 97.8
MS.gene23230.t1 MTR_3g005420 27.820 266 160 7 2 266 305 539 1.02e-22 97.8
MS.gene23230.t1 MTR_3g005420 23.938 259 126 8 2 250 56 253 8.63e-13 68.6
MS.gene23230.t1 MTR_1g021410 25.484 310 177 7 2 262 38 342 1.39e-22 96.3
MS.gene23230.t1 MTR_6g061070 27.000 300 170 12 1 262 33 321 3.42e-21 93.2
MS.gene23230.t1 MTR_1g021400 25.786 318 183 9 2 269 46 360 2.87e-20 89.7
MS.gene23230.t1 MTR_3g005460 25.912 274 163 9 2 266 278 520 3.96e-20 90.5
MS.gene23230.t1 MTR_3g005460 23.346 257 144 5 2 258 41 244 3.42e-17 81.6
MS.gene23230.t1 MTR_1g021435 25.705 319 174 7 2 261 38 352 7.06e-20 88.6
MS.gene23230.t1 MTR_3g005495 30.522 249 127 7 2 250 259 461 2.81e-19 87.8
MS.gene23230.t1 MTR_3g005495 38.372 86 53 0 2 87 29 114 1.10e-17 83.2
MS.gene23230.t1 MTR_3g061220 45.783 83 32 1 2 84 26 95 1.81e-18 79.7
MS.gene23230.t1 MTR_1g021380 23.948 309 178 7 2 264 37 334 9.76e-18 82.4
MS.gene23230.t1 MTR_4g068320 32.867 143 92 2 2 140 22 164 1.32e-17 82.8
MS.gene23230.t1 MTR_4g068320 30.303 132 86 2 137 268 268 393 5.97e-14 72.0
MS.gene23230.t1 MTR_1g034240 42.169 83 48 0 2 84 23 105 3.01e-17 81.6
MS.gene23230.t1 MTR_1g034240 28.667 150 97 3 137 282 272 415 3.77e-13 69.7
MS.gene23230.t1 MTR_1g035480 38.017 121 64 3 1 121 32 141 3.50e-16 75.1
MS.gene23230.t1 MTR_3g005440 37.209 86 54 0 2 87 36 121 6.12e-16 77.8
MS.gene23230.t1 MTR_3g005440 36.047 86 55 0 2 87 258 343 1.02e-11 65.1
MS.gene23230.t1 MTR_1g035650 29.208 202 95 5 2 178 25 203 2.70e-15 73.2
MS.gene23230.t1 MTR_1g035510 35.659 129 68 4 1 129 1 114 9.42e-15 72.0
MS.gene23230.t1 MTR_1g035520 26.531 294 163 11 1 250 87 371 1.94e-14 73.2
MS.gene23230.t1 MTR_1g035470 36.434 129 68 5 1 129 1 115 1.92e-13 66.6
MS.gene23230.t1 MTR_1g034210 39.759 83 48 1 2 84 23 103 2.66e-13 69.3
MS.gene23230.t1 MTR_1g034210 39.759 83 48 1 2 84 23 103 3.69e-13 69.3
MS.gene23230.t1 MTR_3g005420 23.938 259 126 8 2 250 37 234 9.85e-13 68.6
MS.gene23230.t1 MTR_1g021440 38.667 75 46 0 2 76 38 112 4.23e-12 65.5
MS.gene23230.t1 MTR_1g021440 38.667 75 46 0 2 76 38 112 4.73e-12 65.5
MS.gene23230.t1 MTR_1g021440 38.667 75 46 0 2 76 38 112 7.59e-12 65.5
MS.gene23230.t1 MTR_1g021440 38.667 75 46 0 2 76 38 112 7.83e-12 65.5
MS.gene23230.t1 MTR_1g035490 34.711 121 72 4 145 263 81 196 1.36e-11 64.3
MS.gene23230.t1 MTR_1g021320 30.534 131 82 2 2 126 41 168 7.38e-11 63.2
MS.gene23230.t1 MTR_1g021320 29.927 137 88 4 2 132 198 332 8.47e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23230.t1 AT1G49475 41.121 107 60 2 2 106 50 155 2.35e-20 86.7
MS.gene23230.t1 AT4G01580 47.727 88 46 0 2 89 46 133 3.34e-20 86.3
MS.gene23230.t1 AT3G18990 46.988 83 44 0 2 84 22 104 4.97e-20 89.0
MS.gene23230.t1 AT3G18990 46.988 83 44 0 2 84 22 104 6.77e-20 89.0
MS.gene23230.t1 AT3G18990 28.481 158 104 4 109 266 191 339 2.51e-11 63.9
MS.gene23230.t1 AT3G18960 45.455 88 48 0 2 89 46 133 8.27e-19 82.8
MS.gene23230.t1 AT3G18960 45.455 88 48 0 2 89 46 133 1.14e-18 82.8
MS.gene23230.t1 AT3G18960 45.455 88 48 0 2 89 9 96 1.58e-18 81.3
MS.gene23230.t1 AT1G49480 30.709 127 81 3 137 263 102 221 1.02e-11 63.9
MS.gene23230.t1 AT1G49480 30.709 127 81 3 137 263 102 221 1.02e-11 63.9
MS.gene23230.t1 AT1G49480 30.709 127 81 3 137 263 102 221 1.02e-11 63.9

Find 50 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATGGAAATTAAATT+TGG 0.194062 7.2:-52510378 MS.gene23230:CDS
AAAAGGATAATTTATCTCTA+AGG 0.272800 7.2:+52510217 None:intergenic
GAGAGGAGATGTTTATTTGC+AGG 0.289134 7.2:-52508855 MS.gene23230:CDS
TAAATAGTGATGGCAAAATA+TGG 0.289847 7.2:-52510355 MS.gene23230:CDS
CTAATCAGGATGACCCTTAC+AGG 0.321649 7.2:-52508800 MS.gene23230:CDS
TTCCATCAACCACGTGAAAT+AGG 0.327923 7.2:-52510134 MS.gene23230:CDS
AGACTCCAAATGGTGCAAAA+TGG 0.337543 7.2:-52510391 MS.gene23230:CDS
GAGGACAATAACTTGAAAAT+TGG 0.341359 7.2:-52508737 MS.gene23230:CDS
TGGTAATAGACACTTTGATT+TGG 0.367753 7.2:-52508885 MS.gene23230:CDS
ATCCTGATTAGGTACTTTGC+AGG 0.384916 7.2:+52508812 None:intergenic
TGGCAAAATATGGTTTGAAA+AGG 0.393696 7.2:-52510345 MS.gene23230:CDS
AGGTGTTAAATGAAGGAGTT+TGG 0.403488 7.2:-52508835 MS.gene23230:CDS
TTCCTATTTCACGTGGTTGA+TGG 0.411563 7.2:+52510132 None:intergenic
TCAAAGAGTTGCAGCTAAAG+AGG 0.424600 7.2:-52510195 MS.gene23230:CDS
CTGTAAGGGTCATCCTGATT+AGG 0.427523 7.2:+52508801 None:intergenic
GTCTAACATGTGGTCCTGTA+AGG 0.429846 7.2:+52508786 None:intergenic
AAATATGGTTTGAAAAGGGT+TGG 0.440218 7.2:-52510340 MS.gene23230:CDS
GGCCTGCAAAGTACCTAATC+AGG 0.445165 7.2:-52508814 MS.gene23230:CDS
TTAGCTGCAACTCTTTGAAA+AGG 0.448191 7.2:+52510200 None:intergenic
GGACCACATGTTAGACTCTC+AGG 0.469882 7.2:-52508779 MS.gene23230:CDS
TATTTGCAGGTGTTAAATGA+AGG 0.472581 7.2:-52508842 MS.gene23230:CDS
GTTGCAGCTAAAGAGGTATC+TGG 0.485965 7.2:-52510188 MS.gene23230:CDS
GAGAAGCTTCTTCTTTCACT+TGG 0.486869 7.2:+52508520 None:intergenic
TGAAGTTGAGAAATTGCAAA+AGG 0.487851 7.2:-52510096 MS.gene23230:CDS
TCTAAAGATGTGAACAATGT+AGG 0.490187 7.2:+52508673 None:intergenic
GGCAAAATATGGTTTGAAAA+GGG 0.493730 7.2:-52510344 MS.gene23230:CDS
ATATATCTAAAGACTCCAAA+TGG 0.494837 7.2:-52510401 MS.gene23230:CDS
TTAAATTTGGTAAATAGTGA+TGG 0.506736 7.2:-52510365 MS.gene23230:CDS
CATATCTGAAAAGCACAAGA+TGG 0.540339 7.2:+52510282 None:intergenic
TGCAACTTACCAGAAGAAAA+CGG 0.548608 7.2:+52509450 None:intergenic
ATGTTAGACTCTCAGGTGGA+TGG 0.557800 7.2:-52508772 MS.gene23230:CDS
AGAAGCTTCTTCTTTCACTT+GGG 0.559960 7.2:+52508521 None:intergenic
CGCATGACAACAAGAAAGGA+TGG 0.561092 7.2:+52509491 None:intergenic
GAGTATCATTCTCTAGCTCA+TGG 0.575939 7.2:-52510305 MS.gene23230:CDS
CCACGTGAAATAGGAAGCAA+TGG 0.577300 7.2:-52510125 MS.gene23230:CDS
TTTGTGGAGAAATATGGAGA+GGG 0.580289 7.2:-52510437 MS.gene23230:CDS
TTTGATTTGGATCAGAAGAG+AGG 0.591832 7.2:-52508872 MS.gene23230:CDS
TCTAACATGTGGTCCTGTAA+GGG 0.599023 7.2:+52508787 None:intergenic
TAGACTCTCAGGTGGATGGA+AGG 0.601234 7.2:-52508768 MS.gene23230:CDS
CCATTGCTTCCTATTTCACG+TGG 0.606111 7.2:+52510125 None:intergenic
TGCACGCATGACAACAAGAA+AGG 0.606198 7.2:+52509487 None:intergenic
GTGAAAGAAGAAGCTTCTCA+AGG 0.611713 7.2:-52508516 MS.gene23230:CDS
TGGTTGATGGAATTCAAAAG+AGG 0.624709 7.2:+52510145 None:intergenic
CTTTGCATCATGCTGACAGA+GGG 0.633421 7.2:-52510067 MS.gene23230:CDS
GTTTGTGGAGAAATATGGAG+AGG 0.637169 7.2:-52510438 MS.gene23230:CDS
CATGCTGACAGAGGGTCCAG+AGG 0.652976 7.2:-52510059 MS.gene23230:intron
ACTTTGCATCATGCTGACAG+AGG 0.671565 7.2:-52510068 MS.gene23230:CDS
TGGATGGAAGGCATTTGCAG+AGG 0.673309 7.2:-52508756 MS.gene23230:CDS
CCACATGTTAGACTCTCAGG+TGG 0.694586 7.2:-52508776 MS.gene23230:CDS
CCACCTGAGAGTCTAACATG+TGG 0.731917 7.2:+52508776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAGCTAATAATTTAT+AGG + chr7.2:52509300-52509319 None:intergenic 15.0%
!! GATATATTATAATAACATAG+TGG - chr7.2:52509904-52509923 MS.gene23230:intron 15.0%
!! TAAAATAAAAAAATAACACG+TGG - chr7.2:52509111-52509130 MS.gene23230:intron 15.0%
!! TTCACAAGTTAATATAAATT+AGG - chr7.2:52508963-52508982 MS.gene23230:intron 15.0%
!!! TATTTTATTTTTTTTGCCTT+GGG + chr7.2:52509098-52509117 None:intergenic 15.0%
!!! TTAAAAGTACAATTTTTTGA+AGG + chr7.2:52509987-52510006 None:intergenic 15.0%
!!! TTATTTTATTTTTTTTGCCT+TGG + chr7.2:52509099-52509118 None:intergenic 15.0%
!!! TTTTGTAGTTTTTTTTCTTA+GGG - chr7.2:52509224-52509243 MS.gene23230:intron 15.0%
!! AAAAGGATAATTTATCTCTA+AGG + chr7.2:52508750-52508769 None:intergenic 20.0%
!! TGCAAAATGGAAATTAAATT+TGG - chr7.2:52508586-52508605 MS.gene23230:intron 20.0%
!! TTAAATTTGGTAAATAGTGA+TGG - chr7.2:52508599-52508618 MS.gene23230:intron 20.0%
!!! ATTTTATTTTTTTTGCCTTG+GGG + chr7.2:52509097-52509116 None:intergenic 20.0%
!!! GTTTTGTAGTTTTTTTTCTT+AGG - chr7.2:52509223-52509242 MS.gene23230:intron 20.0%
!!! TTTGTAGTTTTTTTTCTTAG+GGG - chr7.2:52509225-52509244 MS.gene23230:intron 20.0%
!!! TTTTGTAGTTTTTTTTTAGG+AGG + chr7.2:52509037-52509056 None:intergenic 20.0%
! AAAAAGTTTGTGGAGAAATA+TGG - chr7.2:52508521-52508540 MS.gene23230:CDS 25.0%
! ATATATCTAAAGACTCCAAA+TGG - chr7.2:52508563-52508582 MS.gene23230:intron 25.0%
! TAAATAGTGATGGCAAAATA+TGG - chr7.2:52508609-52508628 MS.gene23230:intron 25.0%
! TCTATGTGATGATATCTTAA+TGG + chr7.2:52509864-52509883 None:intergenic 25.0%
! TCTCTAGTATTACTAGATTA+AGG - chr7.2:52509595-52509614 MS.gene23230:intron 25.0%
! TGCAAAATTTGAACACAATA+AGG + chr7.2:52509384-52509403 None:intergenic 25.0%
! TGTTAACATAAATCTTTAGC+AGG - chr7.2:52509316-52509335 MS.gene23230:intron 25.0%
! TGTTAGAAGTTAATTAGTAG+AGG - chr7.2:52509702-52509721 MS.gene23230:intron 25.0%
!! TGAAAAAACTTCTCTTTTTG+AGG - chr7.2:52508703-52508722 MS.gene23230:CDS 25.0%
!! TTATTGTGTTCAAATTTTGC+AGG - chr7.2:52509383-52509402 MS.gene23230:intron 25.0%
!!! CGGTTTTGTAGTTTTTTTTT+AGG + chr7.2:52509040-52509059 None:intergenic 25.0%
!!! TATTTCTCCACAAACTTTTT+TGG + chr7.2:52508521-52508540 None:intergenic 25.0%
!!! TTGTAGTTTTTTTTCTTAGG+GGG - chr7.2:52509226-52509245 MS.gene23230:intron 25.0%
!!! TTTTATTTTTTTTGCCTTGG+GGG + chr7.2:52509096-52509115 None:intergenic 25.0%
AAATATGGTTTGAAAAGGGT+TGG - chr7.2:52508624-52508643 MS.gene23230:intron 30.0%
ATCATCACATAGATGTTTAC+TGG - chr7.2:52509871-52509890 MS.gene23230:intron 30.0%
CTACTCTAATTATCCTTAGT+TGG + chr7.2:52509673-52509692 None:intergenic 30.0%
CTAGTAATACTAGAGAGATT+TGG + chr7.2:52509591-52509610 None:intergenic 30.0%
GAGGACAATAACTTGAAAAT+TGG - chr7.2:52510227-52510246 MS.gene23230:CDS 30.0%
GGCAAAATATGGTTTGAAAA+GGG - chr7.2:52508620-52508639 MS.gene23230:intron 30.0%
GTAAGTTGCAAAGAAACATT+TGG - chr7.2:52509527-52509546 MS.gene23230:CDS 30.0%
TATTTGCAGGTGTTAAATGA+AGG - chr7.2:52510122-52510141 MS.gene23230:CDS 30.0%
TCTAAAGATGTGAACAATGT+AGG + chr7.2:52510294-52510313 None:intergenic 30.0%
TGAAGTTGAGAAATTGCAAA+AGG - chr7.2:52508868-52508887 MS.gene23230:CDS 30.0%
TGGCAAAATATGGTTTGAAA+AGG - chr7.2:52508619-52508638 MS.gene23230:intron 30.0%
TGGTAATAGACACTTTGATT+TGG - chr7.2:52510079-52510098 MS.gene23230:CDS 30.0%
! AATTTCCATTTTGCACCATT+TGG + chr7.2:52508581-52508600 None:intergenic 30.0%
! ATCGTAACTTTAGGAAAGTT+AGG - chr7.2:52509254-52509273 MS.gene23230:intron 30.0%
! CATTTGTCTTTTCCTTTGAT+TGG - chr7.2:52510036-52510055 MS.gene23230:intron 30.0%
! CTTTCCTAAAGTTACGATTT+TGG + chr7.2:52509252-52509271 None:intergenic 30.0%
! TCGTAACTTTAGGAAAGTTA+GGG - chr7.2:52509255-52509274 MS.gene23230:intron 30.0%
!!! TTTATTTTTTTTGCCTTGGG+GGG + chr7.2:52509095-52509114 None:intergenic 30.0%
AGAAGCTTCTTCTTTCACTT+GGG + chr7.2:52510446-52510465 None:intergenic 35.0%
AGGTGTTAAATGAAGGAGTT+TGG - chr7.2:52510129-52510148 MS.gene23230:CDS 35.0%
AGTGTTCAACTTCTCAAAGT+AGG - chr7.2:52510336-52510355 MS.gene23230:CDS 35.0%
CTACAGTTGCAAAACTTAAG+GGG + chr7.2:52509063-52509082 None:intergenic 35.0%
GACACAAAATTGTATACCTC+TGG + chr7.2:52508924-52508943 None:intergenic 35.0%
GCTACAGTTGCAAAACTTAA+GGG + chr7.2:52509064-52509083 None:intergenic 35.0%
TACCACAAGTGCAAAACTTA+GGG - chr7.2:52509196-52509215 MS.gene23230:intron 35.0%
TGCAACTTACCAGAAGAAAA+CGG + chr7.2:52509517-52509536 None:intergenic 35.0%
TGCTACAGTTGCAAAACTTA+AGG + chr7.2:52509065-52509084 None:intergenic 35.0%
TGGTATATTCTGCCAATCAA+AGG + chr7.2:52510051-52510070 None:intergenic 35.0%
TGTCTATTACCAAAAATGCG+TGG + chr7.2:52510071-52510090 None:intergenic 35.0%
TTTGTGGAGAAATATGGAGA+GGG - chr7.2:52508527-52508546 MS.gene23230:CDS 35.0%
! CAGAATATACCACGCATTTT+TGG - chr7.2:52510059-52510078 MS.gene23230:intron 35.0%
! CATATCTGAAAAGCACAAGA+TGG + chr7.2:52508685-52508704 None:intergenic 35.0%
! CGTAACTTTAGGAAAGTTAG+GGG - chr7.2:52509256-52509275 MS.gene23230:intron 35.0%
! GTAACTTTAGGAAAGTTAGG+GGG - chr7.2:52509257-52509276 MS.gene23230:intron 35.0%
! TTAGCTGCAACTCTTTGAAA+AGG + chr7.2:52508767-52508786 None:intergenic 35.0%
! TTTTGCAACTGTAGCAGTTT+TGG - chr7.2:52509069-52509088 MS.gene23230:intron 35.0%
!! TGGTTGATGGAATTCAAAAG+AGG + chr7.2:52508822-52508841 None:intergenic 35.0%
!! TTTGATTTGGATCAGAAGAG+AGG - chr7.2:52510092-52510111 MS.gene23230:CDS 35.0%
ACCACAAGTGCAAAACTTAG+GGG - chr7.2:52509197-52509216 MS.gene23230:intron 40.0%
ACCGAGTCAAAATTGACCTT+GGG - chr7.2:52509164-52509183 MS.gene23230:intron 40.0%
AGACTCCAAATGGTGCAAAA+TGG - chr7.2:52508573-52508592 MS.gene23230:intron 40.0%
ATCCTGATTAGGTACTTTGC+AGG + chr7.2:52510155-52510174 None:intergenic 40.0%
ATCGCAACTTCGTGAAAGTT+AGG + chr7.2:52509009-52509028 None:intergenic 40.0%
CAAAAATTGCCCAGCTCAAA+TGG - chr7.2:52509821-52509840 MS.gene23230:intron 40.0%
CTACCACAAGTGCAAAACTT+AGG - chr7.2:52509195-52509214 MS.gene23230:intron 40.0%
GACTATGTATGCACCAACTA+AGG - chr7.2:52509657-52509676 MS.gene23230:intron 40.0%
GAGAAGCTTCTTCTTTCACT+TGG + chr7.2:52510447-52510466 None:intergenic 40.0%
GAGAGGAGATGTTTATTTGC+AGG - chr7.2:52510109-52510128 MS.gene23230:CDS 40.0%
GTGAAAGAAGAAGCTTCTCA+AGG - chr7.2:52510448-52510467 MS.gene23230:CDS 40.0%
GTTTGTGGAGAAATATGGAG+AGG - chr7.2:52508526-52508545 MS.gene23230:CDS 40.0%
TATTTCCCCTTGATACCAGT+TGG - chr7.2:52509787-52509806 MS.gene23230:intron 40.0%
TCAAAGAGTTGCAGCTAAAG+AGG - chr7.2:52508769-52508788 MS.gene23230:CDS 40.0%
TCGCAACTTCGTGAAAGTTA+GGG + chr7.2:52509008-52509027 None:intergenic 40.0%
TCTAACATGTGGTCCTGTAA+GGG + chr7.2:52510180-52510199 None:intergenic 40.0%
TTCCATCAACCACGTGAAAT+AGG - chr7.2:52508830-52508849 MS.gene23230:CDS 40.0%
TTCCTATTTCACGTGGTTGA+TGG + chr7.2:52508835-52508854 None:intergenic 40.0%
! CGTTAAACACCGTTTTCTTC+TGG - chr7.2:52509505-52509524 MS.gene23230:CDS 40.0%
! CTTTCACGAAGTTGCGATTT+TGG - chr7.2:52509011-52509030 MS.gene23230:intron 40.0%
! GAGTATCATTCTCTAGCTCA+TGG - chr7.2:52508659-52508678 MS.gene23230:CDS 40.0%
! TGGGCAATTTTTGCTCAACA+AGG + chr7.2:52509814-52509833 None:intergenic 40.0%
AAATTGCCCAGCTCAAATGG+AGG - chr7.2:52509824-52509843 MS.gene23230:intron 45.0%
AAGGACCAACTGGTATCAAG+GGG + chr7.2:52509795-52509814 None:intergenic 45.0%
AATTGCCCAGCTCAAATGGA+GGG - chr7.2:52509825-52509844 MS.gene23230:intron 45.0%
ACAAGGACCAACTGGTATCA+AGG + chr7.2:52509797-52509816 None:intergenic 45.0%
ACTTTGCATCATGCTGACAG+AGG - chr7.2:52508896-52508915 MS.gene23230:CDS 45.0%
ATGTTAGACTCTCAGGTGGA+TGG - chr7.2:52510192-52510211 MS.gene23230:CDS 45.0%
CAAGGACCAACTGGTATCAA+GGG + chr7.2:52509796-52509815 None:intergenic 45.0%
CAGTTGCAAAACTTAAGGGG+CGG + chr7.2:52509060-52509079 None:intergenic 45.0%
CCACAAGTGCAAAACTTAGG+GGG - chr7.2:52509198-52509217 MS.gene23230:intron 45.0%
CCACGTGAAATAGGAAGCAA+TGG - chr7.2:52508839-52508858 MS.gene23230:CDS 45.0%
CCATTGCTTCCTATTTCACG+TGG + chr7.2:52508842-52508861 None:intergenic 45.0%
CCGAGTCAAAATTGACCTTG+GGG - chr7.2:52509165-52509184 MS.gene23230:intron 45.0%
CGAGTCAAAATTGACCTTGG+GGG - chr7.2:52509166-52509185 MS.gene23230:intron 45.0%
CGCAACTTCGTGAAAGTTAG+GGG + chr7.2:52509007-52509026 None:intergenic 45.0%
CGCATGACAACAAGAAAGGA+TGG + chr7.2:52509476-52509495 None:intergenic 45.0%
CTAATCAGGATGACCCTTAC+AGG - chr7.2:52510164-52510183 MS.gene23230:CDS 45.0%
CTGTAAGGGTCATCCTGATT+AGG + chr7.2:52510166-52510185 None:intergenic 45.0%
CTTTGCATCATGCTGACAGA+GGG - chr7.2:52508897-52508916 MS.gene23230:CDS 45.0%
GACCGAGTCAAAATTGACCT+TGG - chr7.2:52509163-52509182 MS.gene23230:intron 45.0%
GCAACTTCGTGAAAGTTAGG+GGG + chr7.2:52509006-52509025 None:intergenic 45.0%
GGGGCCAAAATCGTAACTTT+AGG - chr7.2:52509245-52509264 MS.gene23230:intron 45.0%
GTCTAACATGTGGTCCTGTA+AGG + chr7.2:52510181-52510200 None:intergenic 45.0%
TAGAACCCTCCATTTGAGCT+GGG + chr7.2:52509833-52509852 None:intergenic 45.0%
TGCACGCATGACAACAAGAA+AGG + chr7.2:52509480-52509499 None:intergenic 45.0%
TTAGAACCCTCCATTTGAGC+TGG + chr7.2:52509834-52509853 None:intergenic 45.0%
TTTGCTCAACAAGGACCAAC+TGG + chr7.2:52509805-52509824 None:intergenic 45.0%
!! GTTGCAGCTAAAGAGGTATC+TGG - chr7.2:52508776-52508795 MS.gene23230:CDS 45.0%
!!! CCCCAAGGTCAATTTTGACT+CGG + chr7.2:52509168-52509187 None:intergenic 45.0%
AACACGTGGCACCTCACTTA+GGG - chr7.2:52509125-52509144 MS.gene23230:intron 50.0%
CAAGTGCAAAACTTAGGGGG+CGG - chr7.2:52509201-52509220 MS.gene23230:intron 50.0%
CCACATGTTAGACTCTCAGG+TGG - chr7.2:52510188-52510207 MS.gene23230:CDS 50.0%
CCACCTGAGAGTCTAACATG+TGG + chr7.2:52510191-52510210 None:intergenic 50.0%
GGACCACATGTTAGACTCTC+AGG - chr7.2:52510185-52510204 MS.gene23230:CDS 50.0%
TAACACGTGGCACCTCACTT+AGG - chr7.2:52509124-52509143 MS.gene23230:intron 50.0%
TAGACTCTCAGGTGGATGGA+AGG - chr7.2:52510196-52510215 MS.gene23230:CDS 50.0%
! CCCCCTAAGTTTTGCACTTG+TGG + chr7.2:52509201-52509220 None:intergenic 50.0%
! GGCCTGCAAAGTACCTAATC+AGG - chr7.2:52510150-52510169 MS.gene23230:CDS 50.0%
!! TGGATGGAAGGCATTTGCAG+AGG - chr7.2:52510208-52510227 MS.gene23230:CDS 50.0%
!!! TGTGGTAGTTTTGACCCCCA+AGG + chr7.2:52509183-52509202 None:intergenic 50.0%
CCTACGTGGTTCCCTAAGTG+AGG + chr7.2:52509139-52509158 None:intergenic 55.0%
CCTCACTTAGGGAACCACGT+AGG - chr7.2:52509136-52509155 MS.gene23230:intron 55.0%
CATGCTGACAGAGGGTCCAG+AGG - chr7.2:52508905-52508924 MS.gene23230:CDS 60.0%
!! GTAGCAGTTTTGGCCCCCCA+AGG - chr7.2:52509079-52509098 MS.gene23230:intron 60.0%
!! TGACTCGGTCTACGCCTACG+TGG + chr7.2:52509153-52509172 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 52508512 52510474 52508512 ID=MS.gene23230
chr7.2 mRNA 52508512 52510474 52508512 ID=MS.gene23230.t1;Parent=MS.gene23230
chr7.2 exon 52510060 52510474 52510060 ID=MS.gene23230.t1.exon1;Parent=MS.gene23230.t1
chr7.2 CDS 52510060 52510474 52510060 ID=cds.MS.gene23230.t1;Parent=MS.gene23230.t1
chr7.2 exon 52509460 52509581 52509460 ID=MS.gene23230.t1.exon2;Parent=MS.gene23230.t1
chr7.2 CDS 52509460 52509581 52509460 ID=cds.MS.gene23230.t1;Parent=MS.gene23230.t1
chr7.2 exon 52508633 52508924 52508633 ID=MS.gene23230.t1.exon3;Parent=MS.gene23230.t1
chr7.2 CDS 52508633 52508924 52508633 ID=cds.MS.gene23230.t1;Parent=MS.gene23230.t1
chr7.2 exon 52508512 52508549 52508512 ID=MS.gene23230.t1.exon4;Parent=MS.gene23230.t1
chr7.2 CDS 52508512 52508549 52508512 ID=cds.MS.gene23230.t1;Parent=MS.gene23230.t1
Gene Sequence

>MS.gene23230

ATGATACCAAAAAAGTTTGTGGAGAAATATGGAGAGGGTTTATCAAATGCTATATATCTAAAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGTAAATAGTGATGGCAAAATATGGTTTGAAAAGGGTTGGAAAGAATTTGCAGAGTATCATTCTCTAGCTCATGGCCATCTTGTGCTTTTCAGATATGAAAAAACTTCTCTTTTTGAGGTACATATCTTTGATAAGAGTGCCTTAGAGATAAATTATCCTTTTCAAAGAGTTGCAGCTAAAGAGGTATCTGGTCAAAAGAGAAAAGCTAACTCCTCTTTTGAATTCCATCAACCACGTGAAATAGGAAGCAATGGTTGTGTTGAAGTTGAGAAATTGCAAAAGGTAGCAACTTTGCATCATGCTGACAGAGGGTCCAGAGGTATACAATTTTGTGTCTTCATTGTCTCATAAAAGTTTCACAAGTTAATATAAATTAGGCTTAATAGCAGTTTTCGCCCCCTAACTTTCACGAAGTTGCGATTTTGGCCTCCTAAAAAAAAACTACAAAACCGCCCCTTAAGTTTTGCAACTGTAGCAGTTTTGGCCCCCCAAGGCAAAAAAAATAAAATAAAAAAATAACACGTGGCACCTCACTTAGGGAACCACGTAGGCGTAGACCGAGTCAAAATTGACCTTGGGGGTCAAAACTACCACAAGTGCAAAACTTAGGGGGCGGTTTTGTAGTTTTTTTTCTTAGGGGGCCAAAATCGTAACTTTAGGAAAGTTAGGGGGCTAAAACTGCTATTAAGCCTATAAATTATTAGCTTTTGTTAACATAAATCTTTAGCAGGTTTCAATATATAGAAACATGAAAGATGATCATGAAATATCAGCCTTATTGTGTTCAAATTTTGCAGGAAAACAAGTGATTACTGCTAAGAAAGTTACAACTCTTGAAAGAGCACAATCTTTCAAAACATGCAATCCATCCTTTCTTGTTGTCATGCGTGCATCATACGTTAAACACCGTTTTCTTCTGGTAAGTTGCAAAGAAACATTTGGCTTTTAGAAAATAAGTTGATAGTCAACGACATCAATAACCAAATCTCTCTAGTATTACTAGATTAAGGTGTGAGAAATATATATTGTTAATTTAAAAATAATTTAAAGACTATGTATGCACCAACTAAGGATAATTAGAGTAGTTAAGAATTTGTTAGAAGTTAATTAGTAGAGGAGATAACTTTGAATAAGTTTTGTAACTCGTTAACACATAGTTTATGTCTTCTTTCTCTTTCATATTTCCCCTTGATACCAGTTGGTCCTTGTTGAGCAAAAATTGCCCAGCTCAAATGGAGGGTTCTAACAATTATCCATTAAGATATCATCACATAGATGTTTACTGGAATATTAGTAGATATATTATAATAACATAGTGGTATTATGATTTGATTAGATAACTATATAAGAAAACACAGTACTTTTTAAAATTAAATCCTTCAAAAAATTGTACTTTTAAACAAGAACTTTGTACTTTTTATTCATTAACATTTGTCTTTTCCTTTGATTGGCAGAATATACCACGCATTTTTGGTAATAGACACTTTGATTTGGATCAGAAGAGAGGAGATGTTTATTTGCAGGTGTTAAATGAAGGAGTTTGGCCTGCAAAGTACCTAATCAGGATGACCCTTACAGGACCACATGTTAGACTCTCAGGTGGATGGAAGGCATTTGCAGAGGACAATAACTTGAAAATTGGCGATGTTTGCAAATTTGAGCTCATCTCTAGCACTATACTTACCTACATTGTTCACATCTTTAGAGAGACAGACAATGACAACACAAAGTGTTCAACTTCTCAAAGTAGGATCAATTGATTTTTAATTTTCATTTCTAATTATTTATATTTTTCATATGACAAATAAATTTATTCTTCTCATATGCAGAATTTTCCCAAGTGAAAGAAGAAGCTTCTCAAGGATAA
Protein sequence

>MS.gene23230.t1

MIPKKFVEKYGEGLSNAIYLKTPNGAKWKLNLVNSDGKIWFEKGWKEFAEYHSLAHGHLVLFRYEKTSLFEVHIFDKSALEINYPFQRVAAKEVSGQKRKANSSFEFHQPREIGSNGCVEVEKLQKVATLHHADRGSRGKQVITAKKVTTLERAQSFKTCNPSFLVVMRASYVKHRFLLNIPRIFGNRHFDLDQKRGDVYLQVLNEGVWPAKYLIRMTLTGPHVRLSGGWKAFAEDNNLKIGDVCKFELISSTILTYIVHIFRETDNDNTKCSTSQKFSQVKEEASQG