Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23536.t1 | XP_024627195.1 | 95.1 | 142 | 7 | 0 | 1 | 142 | 1 | 142 | 3.50E-48 | 201.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23536.t1 | Q9SLD4 | 52.7 | 74 | 32 | 2 | 1 | 71 | 1 | 74 | 6.0e-12 | 72.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23536.t1 | A0A396H512 | 95.1 | 142 | 7 | 0 | 1 | 142 | 1 | 142 | 2.6e-48 | 201.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene23536.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23536.t1 | MTR_7g100100 | 68.531 | 143 | 32 | 3 | 1 | 142 | 3 | 133 | 1.74e-60 | 184 |
| MS.gene23536.t1 | MTR_1g067660 | 61.429 | 140 | 46 | 4 | 5 | 142 | 2 | 135 | 3.70e-52 | 163 |
| MS.gene23536.t1 | MTR_1g067650 | 58.503 | 147 | 46 | 6 | 1 | 142 | 2 | 138 | 4.15e-45 | 145 |
| MS.gene23536.t1 | MTR_1g067640 | 57.823 | 147 | 47 | 6 | 1 | 142 | 1 | 137 | 2.21e-44 | 143 |
| MS.gene23536.t1 | MTR_7g023560 | 52.586 | 116 | 49 | 3 | 1 | 116 | 1 | 110 | 1.46e-32 | 114 |
| MS.gene23536.t1 | MTR_1g115345 | 45.600 | 125 | 52 | 4 | 1 | 115 | 1 | 119 | 1.80e-27 | 101 |
| MS.gene23536.t1 | MTR_2g096540 | 50.459 | 109 | 38 | 3 | 9 | 116 | 4 | 97 | 1.29e-25 | 94.7 |
| MS.gene23536.t1 | MTR_1g062780 | 40.136 | 147 | 47 | 3 | 14 | 119 | 80 | 226 | 2.99e-23 | 92.4 |
| MS.gene23536.t1 | MTR_1g090703 | 36.810 | 163 | 48 | 5 | 11 | 118 | 98 | 260 | 2.30e-22 | 90.9 |
| MS.gene23536.t1 | MTR_2g084315 | 36.000 | 175 | 50 | 6 | 6 | 118 | 62 | 236 | 3.48e-22 | 89.7 |
| MS.gene23536.t1 | MTR_1g106730 | 50.000 | 100 | 44 | 2 | 15 | 114 | 60 | 153 | 4.17e-22 | 88.6 |
| MS.gene23536.t1 | MTR_4g057230 | 41.176 | 119 | 23 | 3 | 44 | 115 | 113 | 231 | 5.64e-20 | 83.6 |
| MS.gene23536.t1 | MTR_1g018420 | 38.462 | 117 | 55 | 2 | 15 | 114 | 43 | 159 | 7.22e-17 | 74.3 |
| MS.gene23536.t1 | MTR_1g077700 | 43.878 | 98 | 39 | 2 | 44 | 125 | 60 | 157 | 9.75e-17 | 73.2 |
| MS.gene23536.t1 | MTR_3g102980 | 40.171 | 117 | 49 | 3 | 15 | 114 | 52 | 164 | 4.89e-16 | 72.4 |
| MS.gene23536.t1 | MTR_0160s0030 | 50.704 | 71 | 33 | 1 | 44 | 114 | 240 | 308 | 7.43e-16 | 73.2 |
| MS.gene23536.t1 | MTR_4g027440 | 47.561 | 82 | 31 | 3 | 44 | 114 | 220 | 300 | 1.85e-15 | 72.0 |
| MS.gene23536.t1 | MTR_5g062300 | 41.489 | 94 | 28 | 3 | 43 | 114 | 118 | 206 | 2.55e-11 | 59.7 |
| MS.gene23536.t1 | MTR_4g073310 | 37.647 | 85 | 45 | 1 | 30 | 114 | 19 | 95 | 3.54e-11 | 58.9 |
| MS.gene23536.t1 | MTR_3g023760 | 42.683 | 82 | 36 | 3 | 44 | 125 | 351 | 421 | 6.40e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23536.t1 | AT2G37430 | 46.575 | 146 | 72 | 4 | 1 | 142 | 1 | 144 | 1.42e-33 | 116 |
| MS.gene23536.t1 | AT3G53600 | 42.384 | 151 | 70 | 4 | 1 | 142 | 1 | 143 | 8.44e-29 | 103 |
| MS.gene23536.t1 | AT2G28710 | 49.138 | 116 | 41 | 6 | 1 | 114 | 1 | 100 | 4.95e-25 | 94.0 |
| MS.gene23536.t1 | AT3G46090 | 47.154 | 123 | 55 | 3 | 1 | 123 | 2 | 114 | 5.90e-25 | 94.0 |
| MS.gene23536.t1 | AT2G28200 | 37.500 | 152 | 56 | 5 | 11 | 125 | 73 | 222 | 8.89e-24 | 93.6 |
| MS.gene23536.t1 | AT3G46080 | 43.548 | 124 | 59 | 3 | 1 | 124 | 2 | 114 | 5.50e-23 | 89.0 |
| MS.gene23536.t1 | AT5G59820 | 45.614 | 114 | 44 | 4 | 6 | 115 | 6 | 105 | 8.24e-23 | 88.2 |
| MS.gene23536.t1 | AT5G03510 | 40.940 | 149 | 46 | 5 | 11 | 117 | 69 | 217 | 1.04e-21 | 88.2 |
| MS.gene23536.t1 | AT5G04390 | 34.969 | 163 | 54 | 5 | 8 | 118 | 93 | 255 | 1.05e-20 | 86.7 |
| MS.gene23536.t1 | AT3G10470 | 45.192 | 104 | 26 | 3 | 44 | 116 | 182 | 285 | 3.46e-19 | 82.8 |
| MS.gene23536.t1 | AT2G45120 | 36.806 | 144 | 72 | 3 | 14 | 138 | 150 | 293 | 4.28e-19 | 81.6 |
| MS.gene23536.t1 | AT1G02030 | 50.000 | 78 | 38 | 1 | 44 | 120 | 160 | 237 | 5.95e-19 | 80.5 |
| MS.gene23536.t1 | AT3G46070 | 39.200 | 125 | 50 | 4 | 1 | 115 | 1 | 109 | 6.31e-19 | 78.6 |
| MS.gene23536.t1 | AT3G49930 | 38.393 | 112 | 57 | 3 | 15 | 114 | 60 | 171 | 8.40e-18 | 76.6 |
| MS.gene23536.t1 | AT3G60580 | 37.398 | 123 | 53 | 3 | 14 | 114 | 126 | 246 | 2.36e-17 | 76.6 |
| MS.gene23536.t1 | AT3G19580 | 38.462 | 117 | 55 | 3 | 15 | 114 | 71 | 187 | 8.56e-17 | 74.7 |
| MS.gene23536.t1 | AT3G19580 | 38.462 | 117 | 55 | 3 | 15 | 114 | 71 | 187 | 8.56e-17 | 74.7 |
| MS.gene23536.t1 | AT5G43170 | 44.000 | 100 | 47 | 3 | 15 | 114 | 50 | 140 | 1.52e-16 | 72.8 |
| MS.gene23536.t1 | AT4G35280 | 38.356 | 146 | 63 | 6 | 11 | 141 | 138 | 271 | 1.75e-16 | 74.3 |
| MS.gene23536.t1 | AT5G04340 | 48.780 | 82 | 31 | 2 | 44 | 114 | 89 | 170 | 1.03e-15 | 71.6 |
| MS.gene23536.t1 | AT1G27730 | 39.815 | 108 | 53 | 3 | 15 | 114 | 55 | 158 | 8.35e-15 | 68.9 |
| MS.gene23536.t1 | AT1G49900 | 41.667 | 108 | 48 | 5 | 22 | 114 | 160 | 267 | 1.37e-14 | 70.1 |
| MS.gene23536.t1 | AT1G49900 | 42.254 | 71 | 41 | 0 | 44 | 114 | 750 | 820 | 3.42e-13 | 65.9 |
| MS.gene23536.t1 | AT2G17180 | 35.714 | 126 | 60 | 3 | 11 | 121 | 121 | 240 | 3.84e-14 | 67.8 |
| MS.gene23536.t1 | AT5G67450 | 37.398 | 123 | 54 | 3 | 15 | 114 | 64 | 186 | 5.46e-14 | 67.0 |
| MS.gene23536.t1 | AT5G56200 | 40.000 | 100 | 39 | 2 | 46 | 124 | 344 | 443 | 1.11e-13 | 67.4 |
| MS.gene23536.t1 | AT1G02040 | 36.170 | 141 | 53 | 3 | 11 | 114 | 107 | 247 | 1.80e-13 | 66.2 |
| MS.gene23536.t1 | AT5G61470 | 43.056 | 72 | 39 | 1 | 43 | 114 | 227 | 296 | 1.58e-11 | 60.8 |
Find 23 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATCTCTTTGTCTCTTCA+TGG | 0.186634 | 7.4:-10100134 | None:intergenic |
| TATTGTTATGTTGTTGATGT+TGG | 0.324104 | 7.4:-10100210 | None:intergenic |
| ATTGTTATGTTGTTGATGTT+GGG | 0.351718 | 7.4:-10100209 | None:intergenic |
| CTATTCAATCTCTTTAAAAC+TGG | 0.356656 | 7.4:-10100532 | None:intergenic |
| AGGATTAGAAAGCATAGATT+TGG | 0.368671 | 7.4:+10100159 | MS.gene23536:CDS |
| CATAAAAGATCAAAGCTAGA+AGG | 0.385523 | 7.4:+10100331 | MS.gene23536:CDS |
| TTCATAACAACTTGCTCCAT+TGG | 0.405809 | 7.4:-10100505 | None:intergenic |
| AATTCTACATCTTTGAGCTT+AGG | 0.417627 | 7.4:+10100370 | MS.gene23536:CDS |
| AAGAGACAAAGAGATATAGA+AGG | 0.447668 | 7.4:+10100139 | MS.gene23536:CDS |
| TGCATGAATGTTCCATTTGT+GGG | 0.475350 | 7.4:+10100407 | MS.gene23536:CDS |
| TTGTTATGTTGTTGATGTTG+GGG | 0.502259 | 7.4:-10100208 | None:intergenic |
| ATGCATGAATGTTCCATTTG+TGG | 0.513081 | 7.4:+10100406 | MS.gene23536:CDS |
| TAAAGAGATTGAATAGTGCA+AGG | 0.521881 | 7.4:+10100539 | MS.gene23536:CDS |
| CAACGAAGAGGTTTCTCCAA+TGG | 0.523977 | 7.4:+10100489 | MS.gene23536:CDS |
| ATAAAAGATCAAAGCTAGAA+GGG | 0.549441 | 7.4:+10100332 | MS.gene23536:CDS |
| AAAGAGATTGAATAGTGCAA+GGG | 0.553931 | 7.4:+10100540 | MS.gene23536:CDS |
| GTGGCCACCTAGTGCTTGAA+AGG | 0.555432 | 7.4:-10100299 | None:intergenic |
| TTCTCATCCTTTCAAGCACT+AGG | 0.596938 | 7.4:+10100292 | MS.gene23536:CDS |
| TAAAGCTATTATCAACGAAG+AGG | 0.599520 | 7.4:+10100477 | MS.gene23536:CDS |
| CATTGGGTGGACACATGAGA+AGG | 0.615753 | 7.4:+10100452 | MS.gene23536:CDS |
| TCATTGGGACAAGCATTGGG+TGG | 0.622140 | 7.4:+10100439 | MS.gene23536:CDS |
| TAAAAGATCAAAGCTAGAAG+GGG | 0.632211 | 7.4:+10100333 | MS.gene23536:CDS |
| TCATCCTTTCAAGCACTAGG+TGG | 0.660073 | 7.4:+10100295 | MS.gene23536:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAAAGATCAAAGCTAGAA+GGG | + | chr7.4:10100332-10100351 | MS.gene23536:CDS | 25.0% |
| ! | ATTGTTATGTTGTTGATGTT+GGG | - | chr7.4:10100212-10100231 | None:intergenic | 25.0% |
| ! | TATTGTTATGTTGTTGATGT+TGG | - | chr7.4:10100213-10100232 | None:intergenic | 25.0% |
| !!! | CTATTCAATCTCTTTAAAAC+TGG | - | chr7.4:10100535-10100554 | None:intergenic | 25.0% |
| AAGAGACAAAGAGATATAGA+AGG | + | chr7.4:10100139-10100158 | MS.gene23536:CDS | 30.0% | |
| AATTCTACATCTTTGAGCTT+AGG | + | chr7.4:10100370-10100389 | MS.gene23536:CDS | 30.0% | |
| CATAAAAGATCAAAGCTAGA+AGG | + | chr7.4:10100331-10100350 | MS.gene23536:CDS | 30.0% | |
| TAAAAGATCAAAGCTAGAAG+GGG | + | chr7.4:10100333-10100352 | MS.gene23536:CDS | 30.0% | |
| TAAAGAGATTGAATAGTGCA+AGG | + | chr7.4:10100539-10100558 | MS.gene23536:CDS | 30.0% | |
| TAAAGCTATTATCAACGAAG+AGG | + | chr7.4:10100477-10100496 | MS.gene23536:CDS | 30.0% | |
| TTGTTATGTTGTTGATGTTG+GGG | - | chr7.4:10100211-10100230 | None:intergenic | 30.0% | |
| ! | AGGATTAGAAAGCATAGATT+TGG | + | chr7.4:10100159-10100178 | MS.gene23536:CDS | 30.0% |
| ! | ATGGAACATTCATGCATTTT+AGG | - | chr7.4:10100403-10100422 | None:intergenic | 30.0% |
| ! | TGAAAATTTTTGCCCACAAA+TGG | - | chr7.4:10100422-10100441 | None:intergenic | 30.0% |
| ! | TGTGGGCAAAAATTTTCATT+GGG | + | chr7.4:10100424-10100443 | MS.gene23536:CDS | 30.0% |
| ! | TTGTGGGCAAAAATTTTCAT+TGG | + | chr7.4:10100423-10100442 | MS.gene23536:CDS | 30.0% |
| !!! | CTTCTAGCTTTGATCTTTTA+TGG | - | chr7.4:10100333-10100352 | None:intergenic | 30.0% |
| ATGCATGAATGTTCCATTTG+TGG | + | chr7.4:10100406-10100425 | MS.gene23536:CDS | 35.0% | |
| TGCATGAATGTTCCATTTGT+GGG | + | chr7.4:10100407-10100426 | MS.gene23536:CDS | 35.0% | |
| ! | ATTTTCATTGGGACAAGCAT+TGG | + | chr7.4:10100435-10100454 | MS.gene23536:CDS | 35.0% |
| ! | TTCATAACAACTTGCTCCAT+TGG | - | chr7.4:10100508-10100527 | None:intergenic | 35.0% |
| ! | TTTTCATTGGGACAAGCATT+GGG | + | chr7.4:10100436-10100455 | MS.gene23536:CDS | 35.0% |
| !!! | GGCGCAAAACTTTTTTGATT+TGG | - | chr7.4:10100238-10100257 | None:intergenic | 35.0% |
| TTCTCATCCTTTCAAGCACT+AGG | + | chr7.4:10100292-10100311 | MS.gene23536:CDS | 40.0% | |
| ! | GTTTTGCACTCATATTCCAC+TGG | - | chr7.4:10100259-10100278 | None:intergenic | 40.0% |
| ! | TCAAAAAAGTTTTGCGCCAG+TGG | + | chr7.4:10100240-10100259 | MS.gene23536:CDS | 40.0% |
| !!! | ATCTTTTATGGCTAGCTCTG+TGG | - | chr7.4:10100321-10100340 | None:intergenic | 40.0% |
| CAACGAAGAGGTTTCTCCAA+TGG | + | chr7.4:10100489-10100508 | MS.gene23536:CDS | 45.0% | |
| TCATCCTTTCAAGCACTAGG+TGG | + | chr7.4:10100295-10100314 | MS.gene23536:CDS | 45.0% | |
| CATTGGGTGGACACATGAGA+AGG | + | chr7.4:10100452-10100471 | MS.gene23536:CDS | 50.0% | |
| !! | TCATTGGGACAAGCATTGGG+TGG | + | chr7.4:10100439-10100458 | MS.gene23536:CDS | 50.0% |
| GTGGCCACCTAGTGCTTGAA+AGG | - | chr7.4:10100302-10100321 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10100136 | 10100561 | 10100136 | ID=MS.gene23536 |
| chr7.4 | mRNA | 10100136 | 10100561 | 10100136 | ID=MS.gene23536.t1;Parent=MS.gene23536 |
| chr7.4 | exon | 10100136 | 10100561 | 10100136 | ID=MS.gene23536.t1.exon1;Parent=MS.gene23536.t1 |
| chr7.4 | CDS | 10100136 | 10100561 | 10100136 | ID=cds.MS.gene23536.t1;Parent=MS.gene23536.t1 |
| Gene Sequence |
| Protein sequence |
