AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23560.t1 MTR_8g036130 86.454 251 28 2 1 249 1 247 2.01e-151 422
MS.gene23560.t1 MTR_4g032620 59.839 249 82 2 1 249 1 231 1.46e-105 305
MS.gene23560.t1 MTR_4g031910 58.233 249 84 4 1 249 1 229 6.95e-90 265
MS.gene23560.t1 MTR_4g032290 56.471 255 81 5 1 249 1 231 7.10e-81 242
MS.gene23560.t1 MTR_3g065100 57.895 247 94 5 1 246 1 238 1.95e-79 239
MS.gene23560.t1 MTR_3g466980 51.807 249 88 6 1 245 1 221 1.64e-76 231
MS.gene23560.t1 MTR_4g032260 70.513 156 46 0 1 156 1 156 7.63e-67 204
MS.gene23560.t1 MTR_2g035610 77.049 122 28 0 1 122 1 122 1.00e-60 187
MS.gene23560.t1 MTR_3g466830 50.314 159 78 1 3 161 2 159 1.33e-54 173
MS.gene23560.t1 MTR_4g063790 44.379 169 94 0 1 169 1 169 9.83e-49 158
MS.gene23560.t1 MTR_3g466890 46.203 158 84 1 3 160 2 158 5.59e-47 154
MS.gene23560.t1 MTR_2g016210 45.238 168 88 1 1 168 1 164 1.15e-45 153
MS.gene23560.t1 MTR_3g467080 43.312 157 89 0 1 157 1 157 2.95e-45 149
MS.gene23560.t1 MTR_5g047580 45.860 157 83 2 1 157 1 155 3.02e-44 146
MS.gene23560.t1 MTR_3g031100 42.675 157 90 0 1 157 1 157 1.35e-42 145
MS.gene23560.t1 MTR_5g075380 40.909 154 90 1 1 154 1 153 3.55e-41 139
MS.gene23560.t1 MTR_3g031240 39.506 162 98 0 1 162 1 162 1.54e-38 133
MS.gene23560.t1 MTR_1g090783 40.385 156 91 2 3 158 2 155 5.28e-37 128
MS.gene23560.t1 MTR_7g011950 44.586 157 85 2 1 157 1 155 2.36e-36 126
MS.gene23560.t1 MTR_1g090697 37.975 158 96 2 1 158 1 156 5.52e-36 125
MS.gene23560.t1 MTR_1g084950 43.949 157 86 2 1 157 1 155 7.80e-36 125
MS.gene23560.t1 MTR_1g077390 39.490 157 93 2 1 157 1 155 1.33e-35 124
MS.gene23560.t1 MTR_1g090710 36.709 158 99 1 1 158 1 157 3.15e-35 123
MS.gene23560.t1 MTR_7g055800 56.303 119 24 2 1 119 1 91 2.86e-34 119
MS.gene23560.t1 MTR_1g077320 42.675 157 88 2 1 157 1 155 5.94e-34 120
MS.gene23560.t1 MTR_3g466900 41.085 129 75 1 32 160 2 129 6.08e-34 119
MS.gene23560.t1 MTR_4g028720 42.675 157 88 2 1 157 1 155 1.56e-33 119
MS.gene23560.t1 MTR_3g466930 41.860 129 74 1 32 160 2 129 9.44e-33 116
MS.gene23560.t1 MTR_3g465410 42.857 126 72 0 29 154 1 126 1.28e-32 116
MS.gene23560.t1 MTR_1g077300 43.949 157 84 2 1 157 1 153 7.40e-32 115
MS.gene23560.t1 MTR_2g035580 41.667 156 90 1 1 156 1 155 2.81e-31 113
MS.gene23560.t1 MTR_7g055790 55.856 111 31 1 1 111 59 151 1.35e-30 111
MS.gene23560.t1 MTR_4g028800 73.611 72 17 1 1 72 1 70 1.80e-30 108
MS.gene23560.t1 MTR_7g055940 55.660 106 19 2 1 106 1 78 3.39e-28 102
MS.gene23560.t1 MTR_2g035590 59.406 101 38 1 74 174 1 98 1.71e-27 101
MS.gene23560.t1 MTR_5g047560 36.306 157 82 3 1 157 1 139 8.27e-26 99.0
MS.gene23560.t1 MTR_4g019670 39.716 141 76 2 1 138 1 135 1.03e-25 105
MS.gene23560.t1 MTR_7g106510 38.298 141 78 2 1 138 1 135 1.68e-25 104
MS.gene23560.t1 MTR_3g093900 33.566 143 95 0 3 145 6 148 5.64e-24 100
MS.gene23560.t1 MTR_4g032270 41.739 115 48 2 135 249 36 131 4.06e-22 89.0
MS.gene23560.t1 MTR_4g084780 69.231 52 16 0 74 125 1 52 8.50e-18 78.2
MS.gene23560.t1 MTR_6g018920 28.125 160 100 6 1 154 1 151 9.76e-14 70.1
MS.gene23560.t1 MTR_1g114730 28.319 113 74 3 1 107 1 112 3.28e-12 65.9
MS.gene23560.t1 MTR_6g005450 31.858 113 70 4 1 107 1 112 4.75e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23560.t1 AT5G48670 53.977 176 79 2 1 175 1 175 3.27e-58 187
MS.gene23560.t1 AT1G65300 44.172 163 90 1 3 165 2 163 4.23e-44 150
MS.gene23560.t1 AT5G26650 43.396 159 89 1 4 162 2 159 4.44e-42 147
MS.gene23560.t1 AT3G05860 44.512 164 90 1 1 164 1 163 7.58e-42 142
MS.gene23560.t1 AT5G26630 46.629 178 94 1 1 178 1 177 1.19e-41 142
MS.gene23560.t1 AT3G05860 40.500 200 109 3 1 199 1 191 1.46e-41 142
MS.gene23560.t1 AT3G05860 44.512 164 90 1 1 164 1 163 2.56e-41 142
MS.gene23560.t1 AT5G27960 42.767 159 90 1 4 162 2 159 2.74e-41 144
MS.gene23560.t1 AT1G65330 41.379 174 99 2 3 174 2 174 4.94e-40 139
MS.gene23560.t1 AT5G27810 59.140 93 38 0 29 121 1 93 8.54e-37 126
MS.gene23560.t1 AT1G31630 36.810 163 101 2 3 165 2 162 2.77e-36 131
MS.gene23560.t1 AT1G31640 37.423 163 100 2 3 165 2 162 7.22e-34 127
MS.gene23560.t1 AT2G28700 37.037 162 97 2 1 160 1 159 1.10e-33 124
MS.gene23560.t1 AT1G22590 33.742 163 104 1 1 163 1 159 5.36e-29 107
MS.gene23560.t1 AT5G26580 36.646 161 87 2 1 161 1 146 7.74e-29 112
MS.gene23560.t1 AT2G40210 40.152 132 79 0 1 132 1 132 5.26e-22 93.6
MS.gene23560.t1 AT5G06500 29.870 154 106 1 1 154 1 152 8.72e-20 85.5
MS.gene23560.t1 AT5G58890 41.964 112 56 4 1 105 1 110 7.18e-16 75.9
MS.gene23560.t1 AT5G55690 35.484 124 71 3 1 115 1 124 3.74e-15 73.6
MS.gene23560.t1 AT5G55690 35.484 124 71 3 1 115 1 124 3.74e-15 73.6
MS.gene23560.t1 AT2G15660 25.000 164 105 4 38 201 31 176 1.42e-12 65.9

Find 56 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGCTTTGTTCCATTTC+AGG 0.136228 8.4:+61295859 None:intergenic
TCCCAATGTCCTTCAAATTT+TGG 0.150964 8.4:+61295650 None:intergenic
GAAGCTTGTGCTATAATTTA+CGG 0.231910 8.4:-61295958 MS.gene23560:CDS
GACTAATCATGATTTGATTC+TGG 0.234615 8.4:+61295587 None:intergenic
CTAGTCTTCAAAGTGGATTT+TGG 0.248575 8.4:-61295372 MS.gene23560:CDS
ATGATGAGTCGTGACCATTT+TGG 0.256307 8.4:-61295499 MS.gene23560:CDS
TTTGGGATAGTTATGAATAA+TGG 0.329950 8.4:-61295481 MS.gene23560:CDS
TCAGACATTGAAACATTAGA+AGG 0.343484 8.4:+61295740 None:intergenic
ATGAACCACAACCTGAGATT+TGG 0.352882 8.4:-61295930 MS.gene23560:CDS
CTTTAGCCTTCATCACCCTT+TGG 0.360586 8.4:+61295809 None:intergenic
ACCAAAATTTGAAGGACATT+GGG 0.367874 8.4:-61295651 MS.gene23560:CDS
ATTCAAGACAATGCCTGAAA+TGG 0.391921 8.4:-61295872 MS.gene23560:CDS
TGAGATTTGGCCATCCCCTT+GGG 0.393251 8.4:-61295917 MS.gene23560:CDS
ATCTCAGGTTGTGGTTCATA+AGG 0.415912 8.4:+61295934 None:intergenic
GAATCATGGGGATGACCAAA+TGG 0.421001 8.4:+61295394 None:intergenic
TATTCATAACTATCCCAAAA+TGG 0.424398 8.4:+61295485 None:intergenic
GACCAAAATTTGAAGGACAT+TGG 0.425243 8.4:-61295652 MS.gene23560:CDS
GGAAAGCAACATACAAGAAA+AGG 0.431751 8.4:-61296032 MS.gene23560:CDS
CTCATTCAAGGTAGTAGATT+AGG 0.433236 8.4:+61295349 None:intergenic
CATGTGATGGTGGTGCCATT+TGG 0.434827 8.4:+61295542 None:intergenic
AATTTGATGATGAATGATAA+TGG 0.435040 8.4:-61295430 MS.gene23560:CDS
GTTGATTGACCAAAATTTGA+AGG 0.435105 8.4:-61295659 MS.gene23560:CDS
CACTTTGAAGACTAGAATCA+TGG 0.444107 8.4:+61295380 None:intergenic
GACGAACTAAGCACACTTTG+TGG 0.444181 8.4:-61295985 MS.gene23560:CDS
CAAGGGGATGGCCAAATCTC+AGG 0.453389 8.4:+61295919 None:intergenic
AATTGAACTTCTCTCTGAGT+TGG 0.458967 8.4:+61295565 None:intergenic
CTGAGATTTGGCCATCCCCT+TGG 0.462588 8.4:-61295918 MS.gene23560:CDS
TTTCACCTTTCTTCTAGTCA+TGG 0.469195 8.4:+61296083 None:intergenic
GAAGGACATTGGGAGAAGGT+TGG 0.475154 8.4:-61295641 MS.gene23560:CDS
TTGGAAGCAGTGGACAATAA+TGG 0.490759 8.4:-61295622 MS.gene23560:CDS
AACATACAAGAAAAGGAAGA+AGG 0.492306 8.4:-61296025 MS.gene23560:CDS
GGAGATGAGAAAGTCCCATT+TGG 0.495533 8.4:-61295409 MS.gene23560:CDS
ATGTTTCAATGTCTGAATGC+AGG 0.499753 8.4:-61295733 MS.gene23560:CDS
TCTTCATTCTTGACATGTGA+TGG 0.500207 8.4:+61295529 None:intergenic
TTGATGTGAATCATCTTACT+TGG 0.506323 8.4:-61295681 MS.gene23560:CDS
CATGAACCAAAGGGTGATGA+AGG 0.509359 8.4:-61295815 MS.gene23560:CDS
AATCATGGGGATGACCAAAT+GGG 0.520667 8.4:+61295395 None:intergenic
TGGGAGAAGGTTGGAAGCAG+TGG 0.521549 8.4:-61295632 MS.gene23560:CDS
GAGAGAAGTTCAATTCCAAA+TGG 0.524320 8.4:-61295557 MS.gene23560:CDS
CAAAACCATGACTAGAAGAA+AGG 0.529842 8.4:-61296088 None:intergenic
TGTTTCAATGTCTGAATGCA+GGG 0.544428 8.4:-61295732 MS.gene23560:CDS
ATTTGAAGGACATTGGGAGA+AGG 0.551499 8.4:-61295645 MS.gene23560:CDS
TGCAGCGTCATTGACAATGA+AGG 0.552900 8.4:+61296056 None:intergenic
ACTTTGAAGACTAGAATCAT+GGG 0.558829 8.4:+61295381 None:intergenic
CATGATTCTAGTCTTCAAAG+TGG 0.564073 8.4:-61295379 MS.gene23560:CDS
AAGAGACTTTCATGAACCAA+AGG 0.569196 8.4:-61295825 MS.gene23560:CDS
GATGGCCAAATCTCAGGTTG+TGG 0.572435 8.4:+61295925 None:intergenic
GGTGATGAAGGCTAAAGAGC+AGG 0.574380 8.4:-61295803 MS.gene23560:CDS
TCATTCTTGACATGTGATGG+TGG 0.582023 8.4:+61295532 None:intergenic
GAGATTTGGCCATCCCCTTG+GGG 0.591825 8.4:-61295916 MS.gene23560:CDS
AGAGACTTTCATGAACCAAA+GGG 0.592104 8.4:-61295824 MS.gene23560:CDS
ACATACAAGAAAAGGAAGAA+GGG 0.593121 8.4:-61296024 MS.gene23560:CDS
TCATTGTCAATGACGCTGCA+AGG 0.601167 8.4:-61296053 MS.gene23560:CDS
CAGACATTGAAACATTAGAA+GGG 0.603894 8.4:+61295741 None:intergenic
CTTTGAAGACTAGAATCATG+GGG 0.693220 8.4:+61295382 None:intergenic
TGTGCAGAACAATATGTCAG+TGG 0.716818 8.4:-61295704 MS.gene23560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGATGATGAATGATAA+TGG - chr8.4:61296005-61296024 MS.gene23560:CDS 20.0%
! AAAACAACAAAAAGACAACA+AGG - chr8.4:61295659-61295678 MS.gene23560:CDS 25.0%
! TATTCATAACTATCCCAAAA+TGG + chr8.4:61295953-61295972 None:intergenic 25.0%
!! TTTGGGATAGTTATGAATAA+TGG - chr8.4:61295954-61295973 MS.gene23560:CDS 25.0%
AACATACAAGAAAAGGAAGA+AGG - chr8.4:61295410-61295429 MS.gene23560:CDS 30.0%
ACATACAAGAAAAGGAAGAA+GGG - chr8.4:61295411-61295430 MS.gene23560:CDS 30.0%
ACCAAAATTTGAAGGACATT+GGG - chr8.4:61295784-61295803 MS.gene23560:CDS 30.0%
CAGACATTGAAACATTAGAA+GGG + chr8.4:61295697-61295716 None:intergenic 30.0%
GAAGCTTGTGCTATAATTTA+CGG - chr8.4:61295477-61295496 MS.gene23560:CDS 30.0%
GACTAATCATGATTTGATTC+TGG + chr8.4:61295851-61295870 None:intergenic 30.0%
GTTGATTGACCAAAATTTGA+AGG - chr8.4:61295776-61295795 MS.gene23560:CDS 30.0%
TCAGACATTGAAACATTAGA+AGG + chr8.4:61295698-61295717 None:intergenic 30.0%
TTGATGTGAATCATCTTACT+TGG - chr8.4:61295754-61295773 MS.gene23560:CDS 30.0%
! ACTTTGAAGACTAGAATCAT+GGG + chr8.4:61296057-61296076 None:intergenic 30.0%
!! TGAATTTTGAGAGCACATTT+TGG + chr8.4:61295548-61295567 None:intergenic 30.0%
AAGAGACTTTCATGAACCAA+AGG - chr8.4:61295610-61295629 MS.gene23560:CDS 35.0%
AATTGAACTTCTCTCTGAGT+TGG + chr8.4:61295873-61295892 None:intergenic 35.0%
AGAGACTTTCATGAACCAAA+GGG - chr8.4:61295611-61295630 MS.gene23560:CDS 35.0%
ATGTTTCAATGTCTGAATGC+AGG - chr8.4:61295702-61295721 MS.gene23560:CDS 35.0%
ATTCAAGACAATGCCTGAAA+TGG - chr8.4:61295563-61295582 MS.gene23560:CDS 35.0%
GACCAAAATTTGAAGGACAT+TGG - chr8.4:61295783-61295802 MS.gene23560:CDS 35.0%
GAGAGAAGTTCAATTCCAAA+TGG - chr8.4:61295878-61295897 MS.gene23560:CDS 35.0%
GGAAAGCAACATACAAGAAA+AGG - chr8.4:61295403-61295422 MS.gene23560:CDS 35.0%
TCTTCATTCTTGACATGTGA+TGG + chr8.4:61295909-61295928 None:intergenic 35.0%
TGTTTCAATGTCTGAATGCA+GGG - chr8.4:61295703-61295722 MS.gene23560:CDS 35.0%
! CACTTTGAAGACTAGAATCA+TGG + chr8.4:61296058-61296077 None:intergenic 35.0%
! CATGATTCTAGTCTTCAAAG+TGG - chr8.4:61296056-61296075 MS.gene23560:CDS 35.0%
! CTTTGAAGACTAGAATCATG+GGG + chr8.4:61296056-61296075 None:intergenic 35.0%
! TCCCAATGTCCTTCAAATTT+TGG + chr8.4:61295788-61295807 None:intergenic 35.0%
! TTCTTGCTTTGTTCCATTTC+AGG + chr8.4:61295579-61295598 None:intergenic 35.0%
!! CTAGTCTTCAAAGTGGATTT+TGG - chr8.4:61296063-61296082 MS.gene23560:CDS 35.0%
AATCATGGGGATGACCAAAT+GGG + chr8.4:61296043-61296062 None:intergenic 40.0%
ATGAACCACAACCTGAGATT+TGG - chr8.4:61295505-61295524 MS.gene23560:CDS 40.0%
ATTTGAAGGACATTGGGAGA+AGG - chr8.4:61295790-61295809 MS.gene23560:CDS 40.0%
TCATTCTTGACATGTGATGG+TGG + chr8.4:61295906-61295925 None:intergenic 40.0%
TGTGCAGAACAATATGTCAG+TGG - chr8.4:61295731-61295750 MS.gene23560:CDS 40.0%
TTGGAAGCAGTGGACAATAA+TGG - chr8.4:61295813-61295832 MS.gene23560:CDS 40.0%
! ATCTCAGGTTGTGGTTCATA+AGG + chr8.4:61295504-61295523 None:intergenic 40.0%
! ATGATGAGTCGTGACCATTT+TGG - chr8.4:61295936-61295955 MS.gene23560:CDS 40.0%
! TGATGAGTCGTGACCATTTT+GGG - chr8.4:61295937-61295956 MS.gene23560:CDS 40.0%
CATGAACCAAAGGGTGATGA+AGG - chr8.4:61295620-61295639 MS.gene23560:CDS 45.0%
GAATCATGGGGATGACCAAA+TGG + chr8.4:61296044-61296063 None:intergenic 45.0%
GACGAACTAAGCACACTTTG+TGG - chr8.4:61295450-61295469 MS.gene23560:CDS 45.0%
GGAGATGAGAAAGTCCCATT+TGG - chr8.4:61296026-61296045 MS.gene23560:CDS 45.0%
TCATTGTCAATGACGCTGCA+AGG - chr8.4:61295382-61295401 MS.gene23560:CDS 45.0%
TGCAGCGTCATTGACAATGA+AGG + chr8.4:61295382-61295401 None:intergenic 45.0%
! CTTTAGCCTTCATCACCCTT+TGG + chr8.4:61295629-61295648 None:intergenic 45.0%
GAAGGACATTGGGAGAAGGT+TGG - chr8.4:61295794-61295813 MS.gene23560:CDS 50.0%
GGTGATGAAGGCTAAAGAGC+AGG - chr8.4:61295632-61295651 MS.gene23560:CDS 50.0%
TGAGATTTGGCCATCCCCTT+GGG - chr8.4:61295518-61295537 MS.gene23560:CDS 50.0%
! AGCACATTTTGGACTCCCCA+AGG + chr8.4:61295537-61295556 None:intergenic 50.0%
! CACATTTTGGACTCCCCAAG+GGG + chr8.4:61295535-61295554 None:intergenic 50.0%
! GATGGCCAAATCTCAGGTTG+TGG + chr8.4:61295513-61295532 None:intergenic 50.0%
! GCACATTTTGGACTCCCCAA+GGG + chr8.4:61295536-61295555 None:intergenic 50.0%
!! CATGTGATGGTGGTGCCATT+TGG + chr8.4:61295896-61295915 None:intergenic 50.0%
CAAGGGGATGGCCAAATCTC+AGG + chr8.4:61295519-61295538 None:intergenic 55.0%
CTGAGATTTGGCCATCCCCT+TGG - chr8.4:61295517-61295536 MS.gene23560:CDS 55.0%
GAGATTTGGCCATCCCCTTG+GGG - chr8.4:61295519-61295538 MS.gene23560:CDS 55.0%
TGGGAGAAGGTTGGAAGCAG+TGG - chr8.4:61295803-61295822 MS.gene23560:CDS 55.0%
! TTTTGGACTCCCCAAGGGGA+TGG + chr8.4:61295531-61295550 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 61295354 61296103 61295354 ID=MS.gene23560
chr8.4 mRNA 61295354 61296103 61295354 ID=MS.gene23560.t1;Parent=MS.gene23560
chr8.4 exon 61295354 61296103 61295354 ID=MS.gene23560.t1.exon1;Parent=MS.gene23560.t1
chr8.4 CDS 61295354 61296103 61295354 ID=cds.MS.gene23560.t1;Parent=MS.gene23560.t1
Gene Sequence

>MS.gene23560

ATGACTAGAAGAAAGGTGAAACTTGCCTTCATTGTCAATGACGCTGCAAGGAAAGCAACATACAAGAAAAGGAAGAAGGGTTTATTGAAGAAGATCGACGAACTAAGCACACTTTGTGGAATTGAAGCTTGTGCTATAATTTACGGTCCTTATGAACCACAACCTGAGATTTGGCCATCCCCTTGGGGAGTCCAAAATGTGCTCTCAAAATTCAAGACAATGCCTGAAATGGAACAAAGCAAGAAAATGATGAATCAAGAGACTTTCATGAACCAAAGGGTGATGAAGGCTAAAGAGCAGGTGAAAAAACAACAAAAAGACAACAAGGAGAAAGAGATGACCCTTCTAATGTTTCAATGTCTGAATGCAGGGAAAATTGTGCAGAACAATATGTCAGTGGTTGATGTGAATCATCTTACTTGGTTGATTGACCAAAATTTGAAGGACATTGGGAGAAGGTTGGAAGCAGTGGACAATAATGGTCAAATTCAAAGCCAGAATCAAATCATGATTAGTCCAACTCAGAGAGAAGTTCAATTCCAAATGGCACCACCATCACATGTCAAGAATGAAGAAATGTCGATGATGAGTCGTGACCATTTTGGGATAGTTATGAATAATGGTGACATCATGCAAAGACAATTCTTTATGAATTTGATGATGAATGATAATGGAGATGAGAAAGTCCCATTTGGTCATCCCCATGATTCTAGTCTTCAAAGTGGATTTTGGCCTAATCTACTACCTTGA

Protein sequence

>MS.gene23560.t1

MTRRKVKLAFIVNDAARKATYKKRKKGLLKKIDELSTLCGIEACAIIYGPYEPQPEIWPSPWGVQNVLSKFKTMPEMEQSKKMMNQETFMNQRVMKAKEQVKKQQKDNKEKEMTLLMFQCLNAGKIVQNNMSVVDVNHLTWLIDQNLKDIGRRLEAVDNNGQIQSQNQIMISPTQREVQFQMAPPSHVKNEEMSMMSRDHFGIVMNNGDIMQRQFFMNLMMNDNGDEKVPFGHPHDSSLQSGFWPNLLP