Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23806.t1 | KEH20354.1 | 100 | 109 | 0 | 0 | 1 | 109 | 1 | 109 | 3.30E-52 | 214.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23806.t1 | P40620 | 86.8 | 68 | 9 | 0 | 42 | 109 | 50 | 117 | 1.1e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23806.t1 | A0A072TSA9 | 100.0 | 109 | 0 | 0 | 1 | 109 | 1 | 109 | 2.4e-52 | 214.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene23806.t1 | TR | HMG |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23806.t1 | MTR_8g073345 | 100.000 | 109 | 0 | 0 | 1 | 109 | 1 | 109 | 5.23e-76 | 221 |
| MS.gene23806.t1 | MTR_8g073345 | 100.000 | 109 | 0 | 0 | 1 | 109 | 1 | 109 | 1.16e-75 | 220 |
| MS.gene23806.t1 | MTR_5g024180 | 68.182 | 110 | 34 | 1 | 1 | 110 | 1 | 109 | 4.40e-48 | 150 |
| MS.gene23806.t1 | MTR_3g115580 | 64.286 | 112 | 34 | 3 | 3 | 108 | 5 | 116 | 8.27e-38 | 125 |
| MS.gene23806.t1 | MTR_7g082820 | 62.651 | 83 | 30 | 1 | 28 | 110 | 4 | 85 | 6.80e-29 | 102 |
| MS.gene23806.t1 | MTR_7g068280 | 63.158 | 76 | 28 | 0 | 33 | 108 | 47 | 122 | 1.55e-28 | 102 |
| MS.gene23806.t1 | MTR_4g094622 | 49.351 | 77 | 39 | 0 | 33 | 109 | 60 | 136 | 8.61e-23 | 87.0 |
| MS.gene23806.t1 | MTR_1g115220 | 51.351 | 74 | 36 | 0 | 33 | 106 | 98 | 171 | 2.11e-22 | 87.4 |
| MS.gene23806.t1 | MTR_5g024180 | 59.091 | 66 | 24 | 1 | 44 | 106 | 3 | 68 | 8.53e-20 | 77.8 |
| MS.gene23806.t1 | MTR_1g022345 | 44.000 | 75 | 41 | 1 | 33 | 107 | 554 | 627 | 1.80e-12 | 62.8 |
| MS.gene23806.t1 | MTR_8g099305 | 44.737 | 76 | 39 | 1 | 33 | 108 | 262 | 334 | 2.32e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23806.t1 | AT1G20696 | 68.539 | 89 | 28 | 0 | 25 | 113 | 23 | 111 | 8.30e-37 | 122 |
| MS.gene23806.t1 | AT1G20696 | 70.930 | 86 | 25 | 0 | 25 | 110 | 23 | 108 | 1.54e-36 | 121 |
| MS.gene23806.t1 | AT1G20693 | 67.391 | 92 | 26 | 1 | 18 | 109 | 23 | 110 | 6.41e-36 | 120 |
| MS.gene23806.t1 | AT1G20693 | 67.391 | 92 | 26 | 1 | 18 | 109 | 23 | 110 | 6.46e-36 | 120 |
| MS.gene23806.t1 | AT1G20693 | 67.391 | 92 | 26 | 1 | 18 | 109 | 23 | 110 | 6.65e-36 | 120 |
| MS.gene23806.t1 | AT1G20696 | 70.588 | 85 | 25 | 0 | 25 | 109 | 23 | 107 | 2.41e-35 | 118 |
| MS.gene23806.t1 | AT2G17560 | 57.143 | 105 | 41 | 1 | 5 | 109 | 7 | 107 | 7.34e-35 | 117 |
| MS.gene23806.t1 | AT2G17560 | 57.143 | 105 | 41 | 1 | 5 | 109 | 7 | 107 | 7.34e-35 | 117 |
| MS.gene23806.t1 | AT2G17560 | 56.075 | 107 | 43 | 1 | 5 | 111 | 7 | 109 | 8.50e-35 | 117 |
| MS.gene23806.t1 | AT4G35570 | 46.789 | 109 | 53 | 1 | 3 | 111 | 5 | 108 | 1.17e-32 | 111 |
| MS.gene23806.t1 | AT4G35570 | 46.789 | 109 | 53 | 1 | 3 | 111 | 5 | 108 | 1.17e-32 | 111 |
| MS.gene23806.t1 | AT3G51880 | 62.353 | 85 | 32 | 0 | 25 | 109 | 41 | 125 | 1.15e-30 | 107 |
| MS.gene23806.t1 | AT3G51880 | 62.353 | 85 | 32 | 0 | 25 | 109 | 41 | 125 | 1.49e-30 | 107 |
| MS.gene23806.t1 | AT3G51880 | 62.353 | 85 | 32 | 0 | 25 | 109 | 41 | 125 | 1.49e-30 | 107 |
| MS.gene23806.t1 | AT3G51880 | 62.353 | 85 | 32 | 0 | 25 | 109 | 41 | 125 | 1.49e-30 | 107 |
| MS.gene23806.t1 | AT3G51880 | 62.353 | 85 | 32 | 0 | 25 | 109 | 41 | 125 | 1.53e-30 | 107 |
| MS.gene23806.t1 | AT1G20696 | 61.538 | 65 | 25 | 0 | 45 | 109 | 1 | 65 | 8.05e-21 | 80.5 |
| MS.gene23806.t1 | AT1G20696 | 61.538 | 65 | 25 | 0 | 45 | 109 | 1 | 65 | 1.03e-20 | 80.1 |
| MS.gene23806.t1 | AT2G34450 | 45.205 | 73 | 40 | 0 | 37 | 109 | 63 | 135 | 6.64e-20 | 79.7 |
| MS.gene23806.t1 | AT2G34450 | 45.205 | 73 | 40 | 0 | 37 | 109 | 63 | 135 | 6.64e-20 | 79.7 |
| MS.gene23806.t1 | AT2G34450 | 45.205 | 73 | 40 | 0 | 37 | 109 | 63 | 135 | 6.64e-20 | 79.7 |
| MS.gene23806.t1 | AT2G34450 | 44.595 | 74 | 40 | 1 | 37 | 109 | 63 | 136 | 2.03e-18 | 75.9 |
| MS.gene23806.t1 | AT5G23420 | 50.000 | 72 | 33 | 2 | 28 | 98 | 106 | 175 | 3.87e-15 | 68.9 |
| MS.gene23806.t1 | AT4G23800 | 42.308 | 78 | 40 | 2 | 33 | 108 | 369 | 443 | 2.43e-11 | 59.3 |
| MS.gene23806.t1 | AT4G23800 | 45.205 | 73 | 37 | 1 | 33 | 105 | 251 | 320 | 4.99e-11 | 58.5 |
| MS.gene23806.t1 | AT4G23800 | 42.308 | 78 | 40 | 2 | 33 | 108 | 375 | 449 | 2.46e-11 | 59.3 |
| MS.gene23806.t1 | AT4G23800 | 45.205 | 73 | 37 | 1 | 33 | 105 | 251 | 320 | 5.20e-11 | 58.5 |
| MS.gene23806.t1 | AT4G11080 | 45.205 | 73 | 37 | 1 | 33 | 105 | 242 | 311 | 6.29e-11 | 58.2 |
Find 24 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGGTCTCTTTGGCTTGTT+AGG | 0.235866 | 8.4:-30909775 | None:intergenic |
| AAAGCACTTGGAGGTCTCTT+TGG | 0.290758 | 8.4:-30909784 | None:intergenic |
| CTTATACTGTTCTCTAAAAT+CGG | 0.321982 | 8.4:-30909937 | None:intergenic |
| ATTCACCAGTTTCTTGTTGT+AGG | 0.325921 | 8.4:-30910364 | None:intergenic |
| GCGGCAACGGATTTGTTGTT+AGG | 0.341320 | 8.4:-30909969 | None:intergenic |
| TTACCGGTTATCAGCGGGTT+TGG | 0.344714 | 8.4:-30909573 | None:intergenic |
| TTGGAATCAGCTTTAACTTT+CGG | 0.360829 | 8.4:-30909554 | None:intergenic |
| AGCTTGAAGCGCAAAGGTGC+TGG | 0.381485 | 8.4:+30909715 | MS.gene23806:intron |
| ATTCCAAACCCGCTGATAAC+CGG | 0.438213 | 8.4:+30909570 | MS.gene23806:CDS |
| ACAAGCCTACAACAAGAAAC+TGG | 0.447828 | 8.4:+30910359 | MS.gene23806:CDS |
| AAGCGCAAAGGTGCTGGAGC+TGG | 0.461656 | 8.4:+30909721 | MS.gene23806:CDS |
| TTCAATGCCCTAGCAGCATA+AGG | 0.463633 | 8.4:-30910309 | None:intergenic |
| GCGATTTACCGGTTATCAGC+GGG | 0.487221 | 8.4:-30909578 | None:intergenic |
| GAGATTTCTTACAGCGGCAA+CGG | 0.506191 | 8.4:-30909982 | None:intergenic |
| CAAGGCTCCTTATGCTGCTA+GGG | 0.507356 | 8.4:+30910302 | MS.gene23806:CDS |
| ACAAGGCTCCTTATGCTGCT+AGG | 0.511412 | 8.4:+30910301 | MS.gene23806:CDS |
| GAAAAGCTTGCGGTGAAGCA+TGG | 0.538505 | 8.4:+30910123 | MS.gene23806:CDS |
| AAAACGAAGAAAGCACTTGG+AGG | 0.558317 | 8.4:-30909793 | None:intergenic |
| ATCAGCTTTAACTTTCGGCA+TGG | 0.563633 | 8.4:-30909549 | None:intergenic |
| ACAGACGAAGAAAGCAGCGA+AGG | 0.587729 | 8.4:+30909750 | MS.gene23806:CDS |
| ATGAAAACGAAGAAAGCACT+TGG | 0.589018 | 8.4:-30909796 | None:intergenic |
| AATTAGGTCGGAAAAGCTTG+CGG | 0.611496 | 8.4:+30910113 | MS.gene23806:intron |
| GCAAAGGTGCTGGAGCTGGA+AGG | 0.622916 | 8.4:+30909725 | MS.gene23806:CDS |
| AGCGATTTACCGGTTATCAG+CGG | 0.628276 | 8.4:-30909579 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATTTATTTTATTTTT+TGG | + | chr8.4:30910026-30910045 | MS.gene23806:intron | 0.0% |
| !!! | TAATTTTTCGAAAATTAATT+AGG | + | chr8.4:30910097-30910116 | MS.gene23806:intron | 10.0% |
| !!! | TTAATTTTAATTGATGATTT+AGG | + | chr8.4:30909913-30909932 | MS.gene23806:intron | 10.0% |
| !! | CTAAAATCAACAAAAAAAAA+TGG | - | chr8.4:30909697-30909716 | None:intergenic | 15.0% |
| !!! | ATTTTTTGGATTATAATTTG+TGG | + | chr8.4:30910040-30910059 | MS.gene23806:intron | 15.0% |
| !!! | TTTTTTTTGTTAACTTTCAT+CGG | + | chr8.4:30910187-30910206 | MS.gene23806:intron | 15.0% |
| !! | CTAATCAGATCTAAAAAAAT+TGG | - | chr8.4:30909629-30909648 | None:intergenic | 20.0% |
| !!! | TAATTTGTGGATTTTATTGT+CGG | + | chr8.4:30910053-30910072 | MS.gene23806:intron | 20.0% |
| !!! | TTTTCGAAAATTAATTAGGT+CGG | + | chr8.4:30910101-30910120 | MS.gene23806:intron | 20.0% |
| ! | CAACCAAAAAAGATTCAAAT+CGG | - | chr8.4:30909852-30909871 | None:intergenic | 25.0% |
| !!! | CTTATACTGTTCTCTAAAAT+CGG | - | chr8.4:30909940-30909959 | None:intergenic | 25.0% |
| !!! | TTTCCGATTTGAATCTTTTT+TGG | + | chr8.4:30909846-30909865 | MS.gene23806:intron | 25.0% |
| TTGGAGAAGAAAAAATCGAA+CGG | - | chr8.4:30909610-30909629 | None:intergenic | 30.0% | |
| ! | TTGGAATCAGCTTTAACTTT+CGG | - | chr8.4:30909557-30909576 | None:intergenic | 30.0% |
| !!! | GTACTGTGATTTTGATTTTC+AGG | + | chr8.4:30910278-30910297 | MS.gene23806:intron | 30.0% |
| !!! | TGATTTTGATTTTCAGGACA+AGG | + | chr8.4:30910284-30910303 | MS.gene23806:intron | 30.0% |
| GTAAACGAGATTTCTTACAG+CGG | - | chr8.4:30909991-30910010 | None:intergenic | 35.0% | |
| ! | ATTCACCAGTTTCTTGTTGT+AGG | - | chr8.4:30910367-30910386 | None:intergenic | 35.0% |
| !! | ATGAAAACGAAGAAAGCACT+TGG | - | chr8.4:30909799-30909818 | None:intergenic | 35.0% |
| AATTAGGTCGGAAAAGCTTG+CGG | + | chr8.4:30910113-30910132 | MS.gene23806:intron | 40.0% | |
| ACAAGCCTACAACAAGAAAC+TGG | + | chr8.4:30910359-30910378 | MS.gene23806:CDS | 40.0% | |
| !! | AAAACGAAGAAAGCACTTGG+AGG | - | chr8.4:30909796-30909815 | None:intergenic | 40.0% |
| !!! | GATTTTAGCTTGAAGCGCAA+AGG | + | chr8.4:30909709-30909728 | MS.gene23806:intron | 40.0% |
| AACGGTGAGAAGCGATTTAC+CGG | - | chr8.4:30909592-30909611 | None:intergenic | 45.0% | |
| ATTCCAAACCCGCTGATAAC+CGG | + | chr8.4:30909570-30909589 | MS.gene23806:CDS | 45.0% | |
| GAGATTTCTTACAGCGGCAA+CGG | - | chr8.4:30909985-30910004 | None:intergenic | 45.0% | |
| TTCAATGCCCTAGCAGCATA+AGG | - | chr8.4:30910312-30910331 | None:intergenic | 45.0% | |
| ! | AAAGCACTTGGAGGTCTCTT+TGG | - | chr8.4:30909787-30909806 | None:intergenic | 45.0% |
| ! | AGCGATTTACCGGTTATCAG+CGG | - | chr8.4:30909582-30909601 | None:intergenic | 45.0% |
| ACAAGGCTCCTTATGCTGCT+AGG | + | chr8.4:30910301-30910320 | MS.gene23806:CDS | 50.0% | |
| ACAGACGAAGAAAGCAGCGA+AGG | + | chr8.4:30909750-30909769 | MS.gene23806:CDS | 50.0% | |
| CAAGGCTCCTTATGCTGCTA+GGG | + | chr8.4:30910302-30910321 | MS.gene23806:CDS | 50.0% | |
| GAAAAGCTTGCGGTGAAGCA+TGG | + | chr8.4:30910123-30910142 | MS.gene23806:CDS | 50.0% | |
| ! | GCGATTTACCGGTTATCAGC+GGG | - | chr8.4:30909581-30909600 | None:intergenic | 50.0% |
| ! | TTACCGGTTATCAGCGGGTT+TGG | - | chr8.4:30909576-30909595 | None:intergenic | 50.0% |
| !! | GCGGCAACGGATTTGTTGTT+AGG | - | chr8.4:30909972-30909991 | None:intergenic | 50.0% |
| !! | GGAGGTCTCTTTGGCTTGTT+AGG | - | chr8.4:30909778-30909797 | None:intergenic | 50.0% |
| ! | AGCTTGAAGCGCAAAGGTGC+TGG | + | chr8.4:30909715-30909734 | MS.gene23806:intron | 55.0% |
| !! | AAGCGCAAAGGTGCTGGAGC+TGG | + | chr8.4:30909721-30909740 | MS.gene23806:CDS | 60.0% |
| !! | GCAAAGGTGCTGGAGCTGGA+AGG | + | chr8.4:30909725-30909744 | MS.gene23806:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 30909551 | 30910401 | 30909551 | ID=MS.gene23806 |
| chr8.4 | mRNA | 30909551 | 30910401 | 30909551 | ID=MS.gene23806.t1;Parent=MS.gene23806 |
| chr8.4 | exon | 30909551 | 30909591 | 30909551 | ID=MS.gene23806.t1.exon1;Parent=MS.gene23806.t1 |
| chr8.4 | CDS | 30909551 | 30909591 | 30909551 | ID=cds.MS.gene23806.t1;Parent=MS.gene23806.t1 |
| chr8.4 | exon | 30909717 | 30909818 | 30909717 | ID=MS.gene23806.t1.exon2;Parent=MS.gene23806.t1 |
| chr8.4 | CDS | 30909717 | 30909818 | 30909717 | ID=cds.MS.gene23806.t1;Parent=MS.gene23806.t1 |
| chr8.4 | exon | 30909935 | 30909992 | 30909935 | ID=MS.gene23806.t1.exon3;Parent=MS.gene23806.t1 |
| chr8.4 | CDS | 30909935 | 30909992 | 30909935 | ID=cds.MS.gene23806.t1;Parent=MS.gene23806.t1 |
| chr8.4 | exon | 30910119 | 30910163 | 30910119 | ID=MS.gene23806.t1.exon4;Parent=MS.gene23806.t1 |
| chr8.4 | CDS | 30910119 | 30910163 | 30910119 | ID=cds.MS.gene23806.t1;Parent=MS.gene23806.t1 |
| chr8.4 | exon | 30910300 | 30910401 | 30910300 | ID=MS.gene23806.t1.exon5;Parent=MS.gene23806.t1 |
| chr8.4 | CDS | 30910300 | 30910401 | 30910300 | ID=cds.MS.gene23806.t1;Parent=MS.gene23806.t1 |
| Gene Sequence |
| Protein sequence |
