AlfalfaGEDB | MS.gene23828

M. sativa cultivar XinJiangDaYe / MS.gene23828

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23828.t1	XP_003629092.1	92.9	170	12	0	1	170	1	170	2.60E-85	325.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23828.t1	Q8S9H7	52.1	48	23	0	103	150	132	179	9.8e-09	62.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23828.t1	G7L8N4	92.9	170	12	0	1	170	1	170	1.9e-85	325.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene23828.t1	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23828.t1	MTR_8g073050	90.625	224	20	1	1	224	1	223	1.76e-128	362
MS.gene23828.t1	MTR_6g043480	59.052	232	84	6	1	224	1	229	1.61e-72	220
MS.gene23828.t1	MTR_6g043490	60.086	233	80	6	1	224	1	229	1.31e-70	215
MS.gene23828.t1	MTR_8g072990	53.785	251	81	8	1	225	1	242	6.54e-65	201
MS.gene23828.t1	MTR_5g027570	40.580	69	41	0	98	166	87	155	5.11e-14	68.9
MS.gene23828.t1	MTR_8g063870	31.387	137	77	3	87	212	65	195	1.76e-12	65.5
MS.gene23828.t1	MTR_2g100930	37.895	95	49	2	56	150	72	156	2.17e-12	65.9
MS.gene23828.t1	MTR_6g092540	32.787	122	64	2	103	224	97	200	2.61e-12	65.1
MS.gene23828.t1	MTR_1g048660	41.333	75	40	1	104	174	13	87	4.09e-12	60.8
MS.gene23828.t1	MTR_4g111975	47.170	53	28	0	100	152	135	187	6.35e-12	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23828.t1	AT3G16350	49.153	59	29	1	93	150	126	184	2.51e-12	65.9
MS.gene23828.t1	AT5G05790	47.273	55	29	0	95	149	123	177	3.44e-12	64.7
MS.gene23828.t1	AT5G05790	47.273	55	29	0	95	149	123	177	3.44e-12	64.7
MS.gene23828.t1	AT5G58900	52.083	48	23	0	103	150	141	188	4.63e-12	64.3
MS.gene23828.t1	AT1G49010	47.170	53	28	0	100	152	131	183	6.18e-12	64.3
MS.gene23828.t1	AT2G38090	52.083	48	23	0	103	150	141	188	8.53e-12	63.5
MS.gene23828.t1	AT5G08520	45.283	53	29	0	100	152	115	167	1.83e-11	62.8
MS.gene23828.t1	AT1G19000	49.091	55	27	1	96	149	94	148	1.95e-11	62.8
MS.gene23828.t1	AT1G19000	49.091	55	27	1	96	149	94	148	1.95e-11	62.8
MS.gene23828.t1	AT5G61620	36.538	104	47	2	104	207	109	193	2.63e-11	62.4

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Find 70 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCATGCACAAATGTGGC+TGG	0.559050	8.4:-31290240	None:intergenic
AAAGATACAGGTGTTGATGG+CGG	0.678925	8.4:-31289259	None:intergenic
AAGACATCCCAATTCTCAAA+TGG	0.506263	8.4:+31289487	MS.gene23828:CDS
AAGATTAGGGTTGGTAGAAA+CGG	0.455787	8.4:-31289314	None:intergenic
AATTGGGATGTCTTTGGTTC+AGG	0.316369	8.4:-31289478	None:intergenic
ACAAGGTAGTTTGGTGTTGG+AGG	0.496753	8.4:-31289354	None:intergenic
ACTCCGCCTAGTGCTTCTGC+TGG	0.556890	8.4:+31290396	MS.gene23828:CDS
ACTCTGCTTCCAAGAGAAAC+AGG	0.420387	8.4:+31290295	MS.gene23828:CDS
AGAAGCACTAGGCGGAGTCA+TGG	0.531695	8.4:-31290391	None:intergenic
AGATTAGGGTTGGTAGAAAC+GGG	0.494754	8.4:-31289313	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATAACTGCGGATTTAATG+AGG	-	chr8.4:31289741-31289760	None:intergenic	30.0%
AGAGAAAGTTGAAATAACTG+CGG	-	chr8.4:31289752-31289771	None:intergenic	30.0%
GGAGCAAAATTAAGAATTGT+TGG	-	chr8.4:31289295-31289314	None:intergenic	30.0%
GGTACATTAGATAAAGATAC+AGG	-	chr8.4:31289274-31289293	None:intergenic	30.0%
GTAAGATCAATGATCTCAAT+AGG	-	chr8.4:31289223-31289242	None:intergenic	30.0%
TATAAAGTAATGTGCAAGCA+TGG	+	chr8.4:31289999-31290018	MS.gene23828:intron	30.0%
AAGACATCCCAATTCTCAAA+TGG	+	chr8.4:31289487-31289506	MS.gene23828:CDS	35.0%
CCACAACAAGTTAAAAGCTA+TGG	+	chr8.4:31290195-31290214	MS.gene23828:CDS	35.0%
GTTTAAGAACCGTTGCAATT+TGG	-	chr8.4:31289453-31289472	None:intergenic	35.0%
TCATCATCTGCTCCATATTA+TGG	+	chr8.4:31290321-31290340	MS.gene23828:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	31289217	31290461	31289217	ID=MS.gene23828
chr8.4	mRNA	31289217	31290461	31289217	ID=MS.gene23828.t1;Parent=MS.gene23828
chr8.4	exon	31289217	31289548	31289217	ID=MS.gene23828.t1.exon1;Parent=MS.gene23828.t1
chr8.4	CDS	31289217	31289548	31289217	ID=cds.MS.gene23828.t1;Parent=MS.gene23828.t1
chr8.4	exon	31290116	31290461	31290116	ID=MS.gene23828.t1.exon2;Parent=MS.gene23828.t1
chr8.4	CDS	31290116	31290461	31290116	ID=cds.MS.gene23828.t1;Parent=MS.gene23828.t1

Gene Sequence

>MS.gene23828

ATGCCTATTGAGATCATTGATCTTACAAATTTCCCTGACTCACCGCCATCAACACCTGTATCTTTATCTAATGTACCAACAATTCTTAATTTTGCTCCCGTTTCTACCAACCCTAATCTTCACCATCAAATCCATCACCTCCAACACCAAACTACCTTGTTTGTACCACCACCTCCAGGATTTGAGACCCCTCAAATCGCTACCGGTTGGCCCCAACCTGACATGTATCACAGCCAAATTGCAACGGTTCTTAAACCGTTACCTGAACCAAAGACATCCCAATTCTCAAATGGAGCTCGGCTCAGGGTTCCTTGGAGTCAATTAGAGCATGAGTAATTACATCTTTCAAACTGAAATCTAATTAATTTTTTATGAAATGATCTCACTTTAATACTGTGAAAAAGTTGAAATTGCGATAAAAATTTAGAGGCACCAAATTTAATGATTAATTTTTTATATTTTTTTCTCTTATTTCCGTAGCTATTATTTTTCTGACAATGTTTTTCTAACTGTCATTTTTACCTCATTAAATCCGCAGTTATTTCAACTTTCTCTTATAGTGCATTTCTTACTTTCATTTCACTATTATAAAACATTGAAATAGCCACAAATATTTGGAGAAAGAAAAATATGAAATTTTTATGTCTCTAATTCTATATTAAATTTTTAGACGCCAAATTTAATGACTAATTTTATTTTTTCGTCTCTAATATCTGTCGTGATTTCAATTTTTGTTTCGATTTTCTTAAAATCACTTCTACACCAATTACTCTTTCATAGATTATAAAGTAATGTGCAAGCATGGATTTTATTTTTCTCCCGTTAAATTTTACTATTGATATTTAATAGACTTAATTTTGATATTTAATAGAATTAATTTTTTATTTTATTTATTTTAGCTTATTTCTGATGGGGCTGATTGAGTATGGAAAAGGGAAATGGAGCAAAATTGCTAAGAACTATGTATCTAGCAAGACACCACAACAAGTTAAAAGCTATGGTTTAAGTTTCTTCATGTACCTACCAGCCACATTTGTGCATGGTTTTAGGAAAAGGAAACTAATTGCAAATCCCAACAACTCTGCTTCCAAGAGAAACAGGAACTCATCATCTGCTCCATATTATGGAGAAGCTAGCAGCAGCAAGAATACCAACAACTATGAAGCAAGCACTTCCATGACTCCGCCTAGTGCTTCTGCTGGTGATGGAGAAGTTGATTTGGAGCTTCGTTTGGGTTTATATTAG

Protein sequence

>MS.gene23828.t1

MPIEIIDLTNFPDSPPSTPVSLSNVPTILNFAPVSTNPNLHHQIHHLQHQTTLFVPPPPGFETPQIATGWPQPDMYHSQIATVLKPLPEPKTSQFSNGARLRVPWSQLEHDLFLMGLIEYGKGKWSKIAKNYVSSKTPQQVKSYGLSFFMYLPATFVHGFRKRKLIANPNNSASKRNRNSSSAPYYGEASSSKNTNNYEASTSMTPPSASAGDGEVDLELRLGLY