AlfalfaGEDB | MS.gene24343

M. sativa cultivar XinJiangDaYe / MS.gene24343

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24343.t1	XP_013466025.1	93.5	186	12	0	1	186	37	222	4.70E-93	350.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24343.t1	Q8RYD1	50.8	120	53	3	2	119	39	154	2.8e-25	116.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24343.t1	A0A072VDG5	93.5	186	12	0	1	186	37	222	3.4e-93	350.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene24343.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24343.t1	MTR_1g021435	93.548	186	12	0	1	186	37	222	4.50e-126	359
MS.gene24343.t1	MTR_1g021410	72.917	192	40	3	1	186	37	222	1.96e-88	263
MS.gene24343.t1	MTR_1g021400	71.503	193	40	5	1	186	45	229	1.10e-84	254
MS.gene24343.t1	MTR_1g021440	57.798	218	60	2	1	186	37	254	3.07e-76	230
MS.gene24343.t1	MTR_1g021350	62.927	205	57	5	1	186	36	240	7.86e-75	228
MS.gene24343.t1	MTR_1g021440	57.798	218	60	2	1	186	37	254	8.02e-75	230
MS.gene24343.t1	MTR_1g021380	63.077	195	48	5	1	186	36	215	2.03e-73	225
MS.gene24343.t1	MTR_1g021440	60.963	187	46	1	1	160	37	223	3.24e-72	219
MS.gene24343.t1	MTR_1g021440	60.963	187	46	1	1	160	37	223	5.83e-71	219
MS.gene24343.t1	MTR_1g021320	50.000	266	53	3	1	186	197	462	2.87e-70	228
MS.gene24343.t1	MTR_1g021320	72.464	138	38	0	1	138	40	177	1.27e-61	204
MS.gene24343.t1	MTR_1g021360	70.807	161	24	3	1	160	197	335	5.00e-69	217
MS.gene24343.t1	MTR_1g021360	69.565	138	42	0	1	138	40	177	9.97e-60	193
MS.gene24343.t1	MTR_1g021330	59.898	197	65	4	1	186	41	234	2.49e-68	213
MS.gene24343.t1	MTR_1g021290	60.000	185	65	2	1	185	170	345	3.98e-67	209
MS.gene24343.t1	MTR_1g021290	78.218	101	22	0	1	101	42	142	3.12e-50	165
MS.gene24343.t1	MTR_1g021500	47.475	198	44	7	1	156	41	220	1.12e-43	150
MS.gene24343.t1	MTR_1g021500	47.475	198	44	7	1	156	41	220	1.61e-43	151
MS.gene24343.t1	MTR_1g021270	37.209	86	53	1	2	87	31	115	4.84e-14	69.7
MS.gene24343.t1	MTR_3g098790	38.667	75	46	0	2	76	29	103	2.00e-13	67.4
MS.gene24343.t1	MTR_7g050580	29.771	131	70	1	2	132	29	137	3.11e-13	67.0
MS.gene24343.t1	MTR_7g050640	36.000	75	48	0	2	76	29	103	7.08e-13	62.8
MS.gene24343.t1	MTR_7g050710	36.486	74	47	0	2	75	29	102	7.65e-13	65.9
MS.gene24343.t1	MTR_7g050720	29.016	193	116	6	2	182	26	209	1.86e-12	64.7
MS.gene24343.t1	MTR_5g070440	38.462	78	48	0	2	79	37	114	2.11e-11	61.2
MS.gene24343.t1	MTR_3g005495	37.333	75	47	0	2	76	259	333	5.08e-11	60.8
MS.gene24343.t1	MTR_7g050590	34.177	79	52	0	2	80	2	80	6.09e-11	60.5
MS.gene24343.t1	MTR_3g005480	37.179	78	49	0	2	79	37	114	9.48e-11	59.7
MS.gene24343.t1	MTR_3g005490	38.667	75	46	0	2	76	37	111	9.89e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24343.t1	AT4G33280	53.398	103	47	1	1	102	38	140	1.72e-32	118
MS.gene24343.t1	AT4G33280	53.398	103	47	1	1	102	38	140	1.07e-31	118
MS.gene24343.t1	AT4G33280	49.593	123	56	3	1	121	38	156	1.20e-31	117
MS.gene24343.t1	AT4G34400	38.824	85	52	0	1	85	30	114	1.71e-18	82.4
MS.gene24343.t1	AT4G34400	38.824	85	52	0	1	85	30	114	1.71e-18	82.4
MS.gene24343.t1	AT5G60130	42.045	88	46	2	1	83	28	115	1.00e-14	70.5
MS.gene24343.t1	AT5G60130	42.045	88	46	2	1	83	2	89	1.23e-14	70.9
MS.gene24343.t1	AT5G60130	42.045	88	46	2	1	83	28	115	1.57e-14	70.5
MS.gene24343.t1	AT5G60130	42.045	88	46	2	1	83	25	112	1.62e-14	70.5
MS.gene24343.t1	AT5G60130	42.045	88	46	2	1	83	28	115	1.70e-14	70.5
MS.gene24343.t1	AT5G66980	45.570	79	39	1	1	75	24	102	3.84e-14	69.7
MS.gene24343.t1	AT3G06220	33.784	74	49	0	2	75	25	98	9.79e-14	66.2
MS.gene24343.t1	AT3G06160	25.949	158	110	3	1	152	24	180	9.31e-13	65.5
MS.gene24343.t1	AT3G06160	25.949	158	110	3	1	152	24	180	9.31e-13	65.5
MS.gene24343.t1	AT3G06160	25.949	158	110	3	1	152	24	180	9.31e-13	65.5
MS.gene24343.t1	AT3G06160	25.949	158	110	3	1	152	39	195	1.00e-12	65.5
MS.gene24343.t1	AT3G06160	25.949	158	110	3	1	152	83	239	1.42e-12	65.5
MS.gene24343.t1	AT1G49475	46.875	64	34	0	13	76	60	123	2.48e-12	62.8
MS.gene24343.t1	AT3G18990	37.333	75	47	0	2	76	22	96	6.79e-12	63.5
MS.gene24343.t1	AT3G18990	37.333	75	47	0	2	76	22	96	8.35e-12	63.2
MS.gene24343.t1	AT3G53310	37.333	75	47	0	2	76	26	100	1.80e-11	61.6
MS.gene24343.t1	AT3G18960	36.559	93	47	2	1	82	34	125	3.39e-11	60.1
MS.gene24343.t1	AT3G18960	36.559	93	47	2	1	82	34	125	5.03e-11	59.7

Find 33 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAGACTTGTTTGTGTCCT+TGG	0.171497	1.1:+2912219	None:intergenic
CAACTCCACTAGGACCTTTA+AGG	0.178570	1.1:+2912473	None:intergenic
ACCTGAGTGTTCTTTGTTAC+AGG	0.205520	1.1:+2911991	None:intergenic
CCAGAAAATGTGACCCTTAA+AGG	0.236805	1.1:-2912487	MS.gene24343:CDS
CCTCCCAATTAACTGGTAAA+AGG	0.296250	1.1:-2912099	MS.gene24343:CDS
TTATGAGAAGTAGCTGCTTT+TGG	0.305223	1.1:+2912034	None:intergenic
ACAAGCGTAAATTTATTAAC+AGG	0.306921	1.1:+2912067	None:intergenic
AATTGGGAGGGAGCTGCAAC+AGG	0.351758	1.1:+2912112	None:intergenic
CGCCATTGTACTTGAAGAAA+AGG	0.367579	1.1:+2912353	None:intergenic
GCATCAGCCATGCACTTCTC+AGG	0.387871	1.1:+2912151	None:intergenic
TCATAAGAGAGCACATGATT+TGG	0.411083	1.1:-2912017	MS.gene24343:CDS
GCAGCTCCCTCCCAATTAAC+TGG	0.415607	1.1:-2912106	MS.gene24343:CDS
AACTCCACTAGGACCTTTAA+GGG	0.479312	1.1:+2912474	None:intergenic
GATACTGTTTACTTCACAAA+CGG	0.486582	1.1:-2912421	MS.gene24343:CDS
AATAAATTTACGCTTGTCCT+CGG	0.500280	1.1:-2912061	MS.gene24343:CDS
TTGGGAGGGAGCTGCAACAG+GGG	0.501107	1.1:+2912114	None:intergenic
GGTCATGCTCAAACCGAACA+AGG	0.526750	1.1:-2912253	MS.gene24343:CDS
TAAGAGAGCACATGATTTGG+TGG	0.528009	1.1:-2912014	MS.gene24343:CDS
CATCAGCCATGCACTTCTCA+GGG	0.533895	1.1:+2912152	None:intergenic
CTGTTTACTTCACAAACGGT+TGG	0.539585	1.1:-2912417	MS.gene24343:CDS
GGAGGGAGCTGCAACAGGGG+TGG	0.557934	1.1:+2912117	None:intergenic
TGGACTTAGTTTCTGTGAGA+AGG	0.559379	1.1:-2912302	MS.gene24343:CDS
TGCTTCCCCTGAGAAGTGCA+TGG	0.578286	1.1:-2912158	MS.gene24343:CDS
ATATTCCACACAACTCCACT+AGG	0.578782	1.1:+2912463	None:intergenic
GTCATGCTCAAACCGAACAA+GGG	0.590087	1.1:-2912252	MS.gene24343:CDS
ATTGGGAGGGAGCTGCAACA+GGG	0.596413	1.1:+2912113	None:intergenic
AAGGTCCTAGTGGAGTTGTG+TGG	0.624686	1.1:-2912468	MS.gene24343:CDS
GTGACCCTTAAAGGTCCTAG+TGG	0.630968	1.1:-2912478	MS.gene24343:CDS
TCATGCTCAAACCGAACAAG+GGG	0.635196	1.1:-2912251	MS.gene24343:CDS
GCCTGTAACAAAGAACACTC+AGG	0.672455	1.1:-2911992	None:intergenic
CAAGGACACAAACAAGTCTG+TGG	0.678532	1.1:-2912218	MS.gene24343:CDS
ATCAGCCATGCACTTCTCAG+GGG	0.683978	1.1:+2912153	None:intergenic
CATGCTCAAACCGAACAAGG+GGG	0.761893	1.1:-2912250	MS.gene24343:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTGTCTGAAAATGTTTT+AGG	+	chr1.1:2912002-2912021	None:intergenic	20.0%
!!!	TTTGAAGTTTTAATCTTTGA+TGG	-	chr1.1:2912200-2912219	MS.gene24343:CDS	20.0%
!	ACAAGCGTAAATTTATTAAC+AGG	+	chr1.1:2912458-2912477	None:intergenic	25.0%
!!!	AATCAATACTACTTTTGATG+CGG	-	chr1.1:2912331-2912350	MS.gene24343:CDS	25.0%
!!!	TCTTATTTTGTTGGAAAATG+TGG	-	chr1.1:2912248-2912267	MS.gene24343:CDS	25.0%
	AATAAATTTACGCTTGTCCT+CGG	-	chr1.1:2912461-2912480	MS.gene24343:CDS	30.0%
	GATACTGTTTACTTCACAAA+CGG	-	chr1.1:2912101-2912120	MS.gene24343:CDS	30.0%
!	GATCCTTTTACCAGTTAATT+GGG	+	chr1.1:2912429-2912448	None:intergenic	30.0%
!	TGATCCTTTTACCAGTTAAT+TGG	+	chr1.1:2912430-2912449	None:intergenic	30.0%
!	TTCCTTTTCTTCAAGTACAA+TGG	-	chr1.1:2912167-2912186	MS.gene24343:CDS	30.0%
!!	ATCAATACTACTTTTGATGC+GGG	-	chr1.1:2912332-2912351	MS.gene24343:CDS	30.0%
!	CTTTTACCAGTTAATTGGGA+GGG	+	chr1.1:2912425-2912444	None:intergenic	35.0%
!	TCATAAGAGAGCACATGATT+TGG	-	chr1.1:2912505-2912524	MS.gene24343:CDS	35.0%
!!	TTATGAGAAGTAGCTGCTTT+TGG	+	chr1.1:2912491-2912510	None:intergenic	35.0%
!!!	AAGGCAGCTTCTTATTTTGT+TGG	-	chr1.1:2912239-2912258	MS.gene24343:CDS	35.0%
	AACTCCACTAGGACCTTTAA+GGG	+	chr1.1:2912051-2912070	None:intergenic	40.0%
	ATATTCCACACAACTCCACT+AGG	+	chr1.1:2912062-2912081	None:intergenic	40.0%
	CCAGAAAATGTGACCCTTAA+AGG	-	chr1.1:2912035-2912054	MS.gene24343:CDS	40.0%
	CCTCCCAATTAACTGGTAAA+AGG	-	chr1.1:2912423-2912442	MS.gene24343:CDS	40.0%
	CGCCATTGTACTTGAAGAAA+AGG	+	chr1.1:2912172-2912191	None:intergenic	40.0%
	CTGTTTACTTCACAAACGGT+TGG	-	chr1.1:2912105-2912124	MS.gene24343:CDS	40.0%
	TGGACTTAGTTTCTGTGAGA+AGG	-	chr1.1:2912220-2912239	MS.gene24343:CDS	40.0%
!	CCTTTTACCAGTTAATTGGG+AGG	+	chr1.1:2912426-2912445	None:intergenic	40.0%
!	TAAGAGAGCACATGATTTGG+TGG	-	chr1.1:2912508-2912527	MS.gene24343:CDS	40.0%
!!	CCTTTAAGGGTCACATTTTC+TGG	+	chr1.1:2912038-2912057	None:intergenic	40.0%
	CAACTCCACTAGGACCTTTA+AGG	+	chr1.1:2912052-2912071	None:intergenic	45.0%
	CAAGGACACAAACAAGTCTG+TGG	-	chr1.1:2912304-2912323	MS.gene24343:CDS	45.0%
	GCCTGTAACAAAGAACACTC+AGG	-	chr1.1:2912530-2912549	MS.gene24343:CDS	45.0%
	GTCATGCTCAAACCGAACAA+GGG	-	chr1.1:2912270-2912289	MS.gene24343:CDS	45.0%
	TCATGCTCAAACCGAACAAG+GGG	-	chr1.1:2912271-2912290	MS.gene24343:CDS	45.0%
!	CACAGACTTGTTTGTGTCCT+TGG	+	chr1.1:2912306-2912325	None:intergenic	45.0%
!!	TACTACTTTTGATGCGGGTG+TGG	-	chr1.1:2912337-2912356	MS.gene24343:CDS	45.0%
!!!	TAGCTGCTTTTGGTAATCCG+AGG	+	chr1.1:2912481-2912500	None:intergenic	45.0%
	AAGGTCCTAGTGGAGTTGTG+TGG	-	chr1.1:2912054-2912073	MS.gene24343:CDS	50.0%
	ATCAGCCATGCACTTCTCAG+GGG	+	chr1.1:2912372-2912391	None:intergenic	50.0%
	CATCAGCCATGCACTTCTCA+GGG	+	chr1.1:2912373-2912392	None:intergenic	50.0%
	CATGCTCAAACCGAACAAGG+GGG	-	chr1.1:2912272-2912291	MS.gene24343:CDS	50.0%
	GGTCATGCTCAAACCGAACA+AGG	-	chr1.1:2912269-2912288	MS.gene24343:CDS	50.0%
	GTGACCCTTAAAGGTCCTAG+TGG	-	chr1.1:2912044-2912063	MS.gene24343:CDS	50.0%
!!	AACCGAACAAGGGGGTATCA+AGG	-	chr1.1:2912280-2912299	MS.gene24343:CDS	50.0%
	AATTGGGAGGGAGCTGCAAC+AGG	+	chr1.1:2912413-2912432	None:intergenic	55.0%
	ATTGGGAGGGAGCTGCAACA+GGG	+	chr1.1:2912412-2912431	None:intergenic	55.0%
	GCAGCTCCCTCCCAATTAAC+TGG	-	chr1.1:2912416-2912435	MS.gene24343:CDS	55.0%
	GCATCAGCCATGCACTTCTC+AGG	+	chr1.1:2912374-2912393	None:intergenic	55.0%
	GGCCTTGATACCCCCTTGTT+CGG	+	chr1.1:2912285-2912304	None:intergenic	55.0%
!	TGCTTCCCCTGAGAAGTGCA+TGG	-	chr1.1:2912364-2912383	MS.gene24343:CDS	55.0%
!!	ACAAGGGGGTATCAAGGCCA+AGG	-	chr1.1:2912286-2912305	MS.gene24343:CDS	55.0%
	TTGGGAGGGAGCTGCAACAG+GGG	+	chr1.1:2912411-2912430	None:intergenic	60.0%
	GGAGGGAGCTGCAACAGGGG+TGG	+	chr1.1:2912408-2912427	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	2911993	2912551	2911993	ID=MS.gene24343
chr1.1	mRNA	2911993	2912551	2911993	ID=MS.gene24343.t1;Parent=MS.gene24343
chr1.1	exon	2911993	2912551	2911993	ID=MS.gene24343.t1.exon1;Parent=MS.gene24343.t1
chr1.1	CDS	2911993	2912551	2911993	ID=cds.MS.gene24343.t1;Parent=MS.gene24343.t1

Gene Sequence

>MS.gene24343

GCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGAGCTGACAACTGAAGATGATACTGTTTACTTCACAAACGGTTGGCAACGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATTTCCTTTTCTTCAAGTACAATGGCGAATCGTTGTTTGAAGTTTTAATCTTTGATGGACTTAGTTTCTGTGAGAAGGCAGCTTCTTATTTTGTTGGAAAATGTGGTCATGCTCAAACCGAACAAGGGGGTATCAAGGCCAAGGACACAAACAAGTCTGTGGAAGAAATCAATACTACTTTTGATGCGGGTGTGGAGTCTGCTTCCCCTGAGAAGTGCATGGCTGATGCAGTCACTAAGACCACCCCTGTTGCAGCTCCCTCCCAATTAACTGGTAAAAGGATCAAGAAGCCTGTTAATAAATTTACGCTTGTCCTCGGATTACCAAAAGCAGCTACTTCTCATAAGAGAGCACATGATTTGGTGGCCTGTAACAAAGAACACTCAG

Protein sequence

>MS.gene24343.t1

ALPKTFSDNLKKKLPENVTLKGPSGVVWNIELTTEDDTVYFTNGWQRFVNDHSLKESDFLFFKYNGESLFEVLIFDGLSFCEKAASYFVGKCGHAQTEQGGIKAKDTNKSVEEINTTFDAGVESASPEKCMADAVTKTTPVAAPSQLTGKRIKKPVNKFTLVLGLPKAATSHKRAHDLVACNKEHS