AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24428

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24428.t1 MTR_1g019120 96.212 396 15 0 45 440 17 412 0.0 745
MS.gene24428.t1 MTR_1g019120 97.643 297 7 0 144 440 1 297 0.0 599
MS.gene24428.t1 MTR_8g076080 50.382 131 63 2 44 173 17 146 1.65e-29 121
MS.gene24428.t1 MTR_8g076080 48.515 101 47 3 335 434 461 557 3.94e-19 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24428.t1 AT1G19860 41.706 422 194 14 45 437 15 413 1.45e-84 265
MS.gene24428.t1 AT1G19860 42.105 418 190 14 49 437 21 415 5.23e-84 264
MS.gene24428.t1 AT5G66270 30.396 227 120 6 46 267 18 211 4.73e-20 92.4
MS.gene24428.t1 AT3G51180 32.906 234 120 8 45 253 19 240 1.10e-18 89.0

Find 98 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTTCAACAGGCGTAAT+TGG 0.151616 1.1:-3939358 None:intergenic
AGAATATTATTGAGTAATTC+AGG 0.252544 1.1:-3939526 None:intergenic
TACATTATCTCCTGGTATAT+TGG 0.266652 1.1:+3939396 MS.gene24428:CDS
GGTGGTCTCAACTTGATTAA+AGG 0.270362 1.1:-3939659 None:intergenic
TCGTCAACATCCACTGAAAT+AGG 0.270410 1.1:-3939301 None:intergenic
TTGCAGGTTCTGACGATTAC+TGG 0.273869 1.1:-3939902 None:intergenic
TCAATAGCTGAACTGATTTC+AGG 0.296986 1.1:-3939778 None:intergenic
GACGATTCCCATTACATTGA+TGG 0.304640 1.1:+3939319 MS.gene24428:CDS
TGATGAGGGATTACCTTGTT+GGG 0.330497 1.1:-3940070 None:intergenic
CTGATGAGGGATTACCTTGT+TGG 0.331743 1.1:-3940071 None:intergenic
TAGTTCAAAGAGAATGAAAA+TGG 0.338707 1.1:+3940101 MS.gene24428:CDS
CCAGCTAATAACTGGTATTT+CGG 0.350750 1.1:-3938687 None:intergenic
TTTGTTGAAGGGACCTCAAC+AGG 0.365719 1.1:-3939808 None:intergenic
CATAGTTTGATAATGGTTGC+AGG 0.368385 1.1:-3939918 None:intergenic
GTGGTCTCAACTTGATTAAA+GGG 0.372939 1.1:-3939658 None:intergenic
TCAACTTGATTAAAGGGATT+AGG 0.374101 1.1:-3939652 None:intergenic
ACCAACTTCTCAATCACTTC+CGG 0.375302 1.1:-3939556 None:intergenic
CAACAAGGTAATCCCTCATC+AGG 0.390234 1.1:+3940072 MS.gene24428:CDS
AACATGACCATCAATGTAAT+GGG 0.405673 1.1:-3939326 None:intergenic
GGTTTAATCCAGCTAATAAC+TGG 0.414260 1.1:-3938695 None:intergenic
CATCTTCAACAGGCGTAATT+GGG 0.418349 1.1:-3939357 None:intergenic
AGGAATCTCCTTCACAAGTT+GGG 0.421056 1.1:+3938534 MS.gene24428:CDS
CCGAAATACCAGTTATTAGC+TGG 0.425996 1.1:+3938687 MS.gene24428:CDS
TCTTCCTATACTCACACAAC+TGG 0.427574 1.1:+3939712 MS.gene24428:CDS
CAACATGACCATCAATGTAA+TGG 0.430043 1.1:-3939327 None:intergenic
TGACGATTACTGGAGGAGTA+TGG 0.441979 1.1:-3939892 None:intergenic
TCCGGAAGTGATTGAGAAGT+TGG 0.446609 1.1:+3939555 MS.gene24428:CDS
CCAATTACGCCTGTTGAAGA+TGG 0.451403 1.1:+3939358 MS.gene24428:CDS
CCCACATCCCCATCTTCAAC+AGG 0.453414 1.1:-3939367 None:intergenic
AGCTCCATTCCGACATCCCC+TGG 0.455307 1.1:-3940013 None:intergenic
GAGGAATCTCCTTCACAAGT+TGG 0.461742 1.1:+3938533 MS.gene24428:CDS
TGGCCCTCCCATGTTGGAAG+TGG 0.472480 1.1:-3937358 None:intergenic
CTCACATGGCAAGTGGTGGC+TGG 0.478796 1.1:+3939094 MS.gene24428:CDS
GCTGTATCATAGTTTGATAA+TGG 0.482378 1.1:-3939925 None:intergenic
CAATTACGCCTGTTGAAGAT+GGG 0.490357 1.1:+3939359 MS.gene24428:CDS
CAATAATATTCTCAACAATC+CGG 0.493677 1.1:+3939537 MS.gene24428:CDS
TTGTTGCCTCAAGGAGAAGC+TGG 0.494944 1.1:+3938596 MS.gene24428:CDS
TTATCTCCTGGTATATTGGA+TGG 0.518348 1.1:+3939400 MS.gene24428:CDS
ATCTCAGCCACTTCCAACAT+GGG 0.525224 1.1:+3937351 MS.gene24428:CDS
TAGCTGGATTAAACCACCTA+AGG 0.527680 1.1:+3938703 MS.gene24428:CDS
GCATGAGTTCCCTCAAATCC+AGG 0.528859 1.1:-3938638 None:intergenic
AGACTCGTACTTTGGAGGAA+TGG 0.532082 1.1:-3939194 None:intergenic
AGAGAAGACTCGTACTTTGG+AGG 0.538641 1.1:-3939199 None:intergenic
TGTGTGAGTATAGGAAGAAG+TGG 0.543096 1.1:-3939707 None:intergenic
ACAAGGATGATATCTTTGCT+TGG 0.550411 1.1:-3938577 None:intergenic
CTGACTTGGCCCTCCCATGT+TGG 0.550437 1.1:-3937364 None:intergenic
GATGAGGGATTACCTTGTTG+GGG 0.550606 1.1:-3940069 None:intergenic
TTAATAGTTCCAGGGGATGT+CGG 0.551000 1.1:+3940004 MS.gene24428:CDS
ACCTCCAGTTGTGTGAGTAT+AGG 0.554188 1.1:-3939716 None:intergenic
GCCTGTTGAAGATGGGGATG+TGG 0.558484 1.1:+3939366 MS.gene24428:CDS
TGAACTATGCATCCCTGATG+AGG 0.559066 1.1:-3940085 None:intergenic
GATCTCAGCCACTTCCAACA+TGG 0.561348 1.1:+3937350 MS.gene24428:CDS
GCACAGTATGCACAAAATAC+CGG 0.562338 1.1:+3939601 MS.gene24428:CDS
TGAAGATGGGGATGTGGGAG+CGG 0.563372 1.1:+3939372 MS.gene24428:CDS
CAGAAAATATCACACTGACT+TGG 0.563490 1.1:-3937378 None:intergenic
CTAATAACTGGTATTTCGGA+CGG 0.563575 1.1:-3938683 None:intergenic
GGTTAGATTATTCTTATCTG+AGG 0.564458 1.1:+3938514 MS.gene24428:intron
GAACTATGCATCCCTGATGA+GGG 0.564645 1.1:-3940084 None:intergenic
CGTCAACTATGTCTACTCAA+TGG 0.565260 1.1:+3939746 MS.gene24428:CDS
AGCTTCTCCTTGAGGCAACA+AGG 0.565922 1.1:-3938594 None:intergenic
TATTGATGCTACTCCTGTTG+AGG 0.566975 1.1:+3939795 MS.gene24428:CDS
GGAGCGGATACATTATCTCC+TGG 0.567885 1.1:+3939388 MS.gene24428:CDS
ACTTGATTAAAGGGATTAGG+AGG 0.568417 1.1:-3939649 None:intergenic
TGTCTACTCAATGGCATCGT+AGG 0.571059 1.1:+3939755 MS.gene24428:CDS
GCTGGTGCTTGCAGAAGTAT+CGG 0.571164 1.1:+3937411 MS.gene24428:CDS
AGTTCCAGGGGATGTCGGAA+TGG 0.577472 1.1:+3940009 MS.gene24428:CDS
GATATTTGATCAACATCAGA+GGG 0.578943 1.1:+3939132 MS.gene24428:CDS
GAATTCAACCCAACTTGTGA+AGG 0.578955 1.1:-3938542 None:intergenic
TCACACAACTGGAGGTCAGA+TGG 0.580334 1.1:+3939723 MS.gene24428:CDS
CACTGTTACGACTATGTCGA+TGG 0.580449 1.1:+3939429 MS.gene24428:CDS
AGGATGATATCTTTGCTTGG+AGG 0.580593 1.1:-3938574 None:intergenic
ATATCATCCTTGTTGCCTCA+AGG 0.583248 1.1:+3938587 MS.gene24428:CDS
TACTTGAAGCTATTTATCCA+CGG 0.587446 1.1:+3939164 MS.gene24428:CDS
TCGATGCAGTCCTATTTCAG+TGG 0.588300 1.1:+3939291 MS.gene24428:intron
AAAAGCATTTGATGAGGATG+TGG 0.594665 1.1:-3939680 None:intergenic
AAGAGCTTGATTCAGCAACA+TGG 0.600253 1.1:+3939847 MS.gene24428:CDS
TCAGAACCAGCTTCTCCTTG+AGG 0.604428 1.1:-3938602 None:intergenic
TCCTATACTCACACAACTGG+AGG 0.607401 1.1:+3939715 MS.gene24428:CDS
CAGCCACTTCCAACATGGGA+GGG 0.629186 1.1:+3937355 MS.gene24428:CDS
TTTGAACCATCCAATATACC+AGG 0.631062 1.1:-3939406 None:intergenic
CCTGTTGAAGATGGGGATGT+GGG 0.634741 1.1:+3939367 MS.gene24428:CDS
GCTAGATCTCACATGGCAAG+TGG 0.640415 1.1:+3939087 MS.gene24428:CDS
GATACTTCTGCAAGCACCAG+CGG 0.641670 1.1:-3937409 None:intergenic
AAATGATGCATCATGCTGAA+AGG 0.642980 1.1:-3940043 None:intergenic
TCAGCCACTTCCAACATGGG+AGG 0.643487 1.1:+3937354 MS.gene24428:CDS
GCATCATTTCAGCCCCAACA+AGG 0.644422 1.1:+3940057 MS.gene24428:CDS
AGATATTTGATCAACATCAG+AGG 0.644462 1.1:+3939131 MS.gene24428:CDS
AGATCTCACATGGCAAGTGG+TGG 0.645516 1.1:+3939090 MS.gene24428:CDS
AGATCGTGCTAGATCTCACA+TGG 0.650447 1.1:+3939080 MS.gene24428:intron
TTAGTGAAGAGCAGTGAACA+TGG 0.653142 1.1:+3939490 MS.gene24428:CDS
AATTACGCCTGTTGAAGATG+GGG 0.658485 1.1:+3939360 MS.gene24428:CDS
AGCATTTGATGAGGATGTGG+TGG 0.660304 1.1:-3939677 None:intergenic
AACAAGGTAATCCCTCATCA+GGG 0.662054 1.1:+3940073 MS.gene24428:CDS
CAGGTTCTGACGATTACTGG+AGG 0.674373 1.1:-3939899 None:intergenic
GGAGGAATGGAAGATATCCG+TGG 0.678525 1.1:-3939181 None:intergenic
GGTGCTTGCAGAAGTATCGG+AGG 0.700785 1.1:+3937414 MS.gene24428:CDS
AGCTTGATTCAGCAACATGG+AGG 0.714964 1.1:+3939850 MS.gene24428:CDS
TGAGTTCCCTCAAATCCAGG+AGG 0.756506 1.1:-3938635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATATTAATAATATATG+TGG - chr1.1:3938048-3938067 None:intergenic 10.0%
!! TTTCTTATTCAAATTATTAT+GGG - chr1.1:3937850-3937869 None:intergenic 10.0%
!! AAAAAAAGAAATATCAAACA+AGG - chr1.1:3939227-3939246 None:intergenic 15.0%
!! CTTTCTTATTCAAATTATTA+TGG - chr1.1:3937851-3937870 None:intergenic 15.0%
!! TAATATTTATATTTCAACGT+TGG + chr1.1:3938771-3938790 MS.gene24428:intron 15.0%
!! AATATTACTGAAGATTACTA+TGG - chr1.1:3938758-3938777 None:intergenic 20.0%
!! AGAATATTATTGAGTAATTC+AGG - chr1.1:3939529-3939548 None:intergenic 20.0%
!! ATTATGGGTAATCATAATAA+AGG - chr1.1:3937835-3937854 None:intergenic 20.0%
!! GAACTATTAAAAAAGATACA+AGG - chr1.1:3939994-3940013 None:intergenic 20.0%
!! TAAAATATGACAGTAAAAGA+TGG - chr1.1:3938121-3938140 None:intergenic 20.0%
!! TATAACTACAAAAATACCTT+AGG - chr1.1:3938722-3938741 None:intergenic 20.0%
!! TATTTATTGTGCATTCAAAA+TGG + chr1.1:3937910-3937929 MS.gene24428:intron 20.0%
!! TTTATGAATATTGTAGACTA+GGG - chr1.1:3937725-3937744 None:intergenic 20.0%
!!! TATCTTTTTTAATAGTTCCA+GGG + chr1.1:3939996-3940015 MS.gene24428:CDS 20.0%
!!! TTTTATGAATATTGTAGACT+AGG - chr1.1:3937726-3937745 None:intergenic 20.0%
! CAAAATGGCTATAAATATTC+TGG + chr1.1:3937925-3937944 MS.gene24428:intron 25.0%
! CAATAATATTCTCAACAATC+CGG + chr1.1:3939537-3939556 MS.gene24428:CDS 25.0%
! CATAACTCGAAAAAAAAAGA+AGG + chr1.1:3938990-3939009 MS.gene24428:intron 25.0%
! GTACTTTATAATGAACATCT+GGG - chr1.1:3938444-3938463 None:intergenic 25.0%
! TAGTTCAAAGAGAATGAAAA+TGG + chr1.1:3940101-3940120 MS.gene24428:CDS 25.0%
! TATCTCTAGTATGAATCTTA+TGG + chr1.1:3937785-3937804 MS.gene24428:intron 25.0%
! TGTACTTTATAATGAACATC+TGG - chr1.1:3938445-3938464 None:intergenic 25.0%
! TTATGAATATTGTAGACTAG+GGG - chr1.1:3937724-3937743 None:intergenic 25.0%
!! ACAAAGAAATAAACAGGTTT+TGG - chr1.1:3938241-3938260 None:intergenic 25.0%
!! AGTTTACATCTTTTGTTGAA+GGG - chr1.1:3939822-3939841 None:intergenic 25.0%
!! TAGTTTACATCTTTTGTTGA+AGG - chr1.1:3939823-3939842 None:intergenic 25.0%
!! TGTTCTTTTTCTGTTTCAAA+AGG + chr1.1:3937314-3937333 MS.gene24428:CDS 25.0%
!! TTTTAATCAGGATCTTAACT+TGG + chr1.1:3937625-3937644 MS.gene24428:intron 25.0%
!!! AAAAACATTTGATGATGATC+CGG - chr1.1:3939623-3939642 None:intergenic 25.0%
!!! ATCTGTTTTCACTTTTAATC+AGG + chr1.1:3937613-3937632 MS.gene24428:intron 25.0%
!!! ATCTTTTTTAATAGTTCCAG+GGG + chr1.1:3939997-3940016 MS.gene24428:CDS 25.0%
!!! CATATTATAAGAGTGCTAAA+AGG + chr1.1:3938463-3938482 MS.gene24428:intron 25.0%
!!! GTATCTTTTTTAATAGTTCC+AGG + chr1.1:3939995-3940014 MS.gene24428:CDS 25.0%
!!! TTTTGAAAAAGCTTGCAATT+TGG + chr1.1:3937582-3937601 MS.gene24428:intron 25.0%
AACATGACCATCAATGTAAT+GGG - chr1.1:3939329-3939348 None:intergenic 30.0%
AACTACAAAAATACCTTAGG+TGG - chr1.1:3938719-3938738 None:intergenic 30.0%
ACAGTATGCCAATATATCAT+CGG + chr1.1:3938913-3938932 MS.gene24428:intron 30.0%
AGATATTTGATCAACATCAG+AGG + chr1.1:3939131-3939150 MS.gene24428:CDS 30.0%
ATCTAACCTGTTCAACAAAA+AGG - chr1.1:3938502-3938521 None:intergenic 30.0%
GAAAAAACCGCTATATACAT+AGG - chr1.1:3938865-3938884 None:intergenic 30.0%
GATATTTGATCAACATCAGA+GGG + chr1.1:3939132-3939151 MS.gene24428:CDS 30.0%
GCTGTATCATAGTTTGATAA+TGG - chr1.1:3939928-3939947 None:intergenic 30.0%
GGTTAGATTATTCTTATCTG+AGG + chr1.1:3938514-3938533 MS.gene24428:intron 30.0%
GTAAAGTTGACAGAAATTCT+TGG + chr1.1:3937436-3937455 MS.gene24428:intron 30.0%
TACATTATCTCCTGGTATAT+TGG + chr1.1:3939396-3939415 MS.gene24428:CDS 30.0%
TACTTGAAGCTATTTATCCA+CGG + chr1.1:3939164-3939183 MS.gene24428:CDS 30.0%
TCAACTTGATTAAAGGGATT+AGG - chr1.1:3939655-3939674 None:intergenic 30.0%
TCATACTAGAGATACTTAGA+AGG - chr1.1:3937779-3937798 None:intergenic 30.0%
TCTAGTATGAATCTTATGGA+TGG + chr1.1:3937789-3937808 MS.gene24428:intron 30.0%
TGTCAAACCTATGTATATAG+CGG + chr1.1:3938855-3938874 MS.gene24428:intron 30.0%
! GTTGTCTGAAGTGAATTAAT+TGG - chr1.1:3937470-3937489 None:intergenic 30.0%
!! ATAAGAGTGCTAAAAGGAAA+AGG + chr1.1:3938469-3938488 MS.gene24428:intron 30.0%
!! GTATGTATTTCTAGGTTGTT+GGG + chr1.1:3938937-3938956 MS.gene24428:intron 30.0%
!! TAGTAGAAGATATTGATTCC+TGG + chr1.1:3937753-3937772 MS.gene24428:intron 30.0%
AAATGATGCATCATGCTGAA+AGG - chr1.1:3940046-3940065 None:intergenic 35.0%
AATGCAGAGTTATAGAGAGA+AGG + chr1.1:3937660-3937679 MS.gene24428:intron 35.0%
ACAAGGATGATATCTTTGCT+TGG - chr1.1:3938580-3938599 None:intergenic 35.0%
ACTTGATTAAAGGGATTAGG+AGG - chr1.1:3939652-3939671 None:intergenic 35.0%
AGTATGCCAATATATCATCG+GGG + chr1.1:3938915-3938934 MS.gene24428:intron 35.0%
AGTTGACAGAAATTCTTGGT+TGG + chr1.1:3937440-3937459 MS.gene24428:intron 35.0%
ATGCAGAGTTATAGAGAGAA+GGG + chr1.1:3937661-3937680 MS.gene24428:intron 35.0%
CAACATGACCATCAATGTAA+TGG - chr1.1:3939330-3939349 None:intergenic 35.0%
CACAGCACAAAGAAATAAAC+AGG - chr1.1:3938247-3938266 None:intergenic 35.0%
CAGTATGCCAATATATCATC+GGG + chr1.1:3938914-3938933 MS.gene24428:intron 35.0%
CCAGCTAATAACTGGTATTT+CGG - chr1.1:3938690-3938709 None:intergenic 35.0%
CTAATAACTGGTATTTCGGA+CGG - chr1.1:3938686-3938705 None:intergenic 35.0%
GAGGCTATATAAGTTGCAAT+TGG + chr1.1:3937502-3937521 MS.gene24428:intron 35.0%
GGTTTAATCCAGCTAATAAC+TGG - chr1.1:3938698-3938717 None:intergenic 35.0%
GTGGTCTCAACTTGATTAAA+GGG - chr1.1:3939661-3939680 None:intergenic 35.0%
TACATACCCCGATGATATAT+TGG - chr1.1:3938924-3938943 None:intergenic 35.0%
TATAACTCTGCATTGAGACT+GGG - chr1.1:3937654-3937673 None:intergenic 35.0%
TTATATAGCCTCACTATGAC+AGG - chr1.1:3937494-3937513 None:intergenic 35.0%
TTATCTCCTGGTATATTGGA+TGG + chr1.1:3939400-3939419 MS.gene24428:CDS 35.0%
TTTGAACCATCCAATATACC+AGG - chr1.1:3939409-3939428 None:intergenic 35.0%
! ATTTTACCTCCTGGATTTGA+GGG + chr1.1:3938629-3938648 MS.gene24428:CDS 35.0%
! CAAGTCAGTGTGATATTTTC+TGG + chr1.1:3937379-3937398 MS.gene24428:CDS 35.0%
! CAGAAAATATCACACTGACT+TGG - chr1.1:3937381-3937400 None:intergenic 35.0%
! CTGTTTCCTTTTTGTTGAAC+AGG + chr1.1:3938493-3938512 MS.gene24428:intron 35.0%
! TATTTTACCTCCTGGATTTG+AGG + chr1.1:3938628-3938647 MS.gene24428:CDS 35.0%
! TCAATAGCTGAACTGATTTC+AGG - chr1.1:3939781-3939800 None:intergenic 35.0%
! TCTGATGATATTTTACCTCC+TGG + chr1.1:3938620-3938639 MS.gene24428:CDS 35.0%
!! AAAAGCATTTGATGAGGATG+TGG - chr1.1:3939683-3939702 None:intergenic 35.0%
!! ATACAAGGCTTCGTTGTTTT+TGG - chr1.1:3939979-3939998 None:intergenic 35.0%
!! CATAGTTTGATAATGGTTGC+AGG - chr1.1:3939921-3939940 None:intergenic 35.0%
!! GGATGAAAAAGCATTTGATG+AGG - chr1.1:3939689-3939708 None:intergenic 35.0%
!! GGTATGTATTTCTAGGTTGT+TGG + chr1.1:3938936-3938955 MS.gene24428:intron 35.0%
!!! AAACAGGTTTTGGATGAAAG+TGG - chr1.1:3938231-3938250 None:intergenic 35.0%
!!! GCACACAATTTTAGAGCAAA+AGG + chr1.1:3939946-3939965 MS.gene24428:CDS 35.0%
AACAAGGTAATCCCTCATCA+GGG + chr1.1:3940073-3940092 MS.gene24428:CDS 40.0%
AAGAGCTTGATTCAGCAACA+TGG + chr1.1:3939847-3939866 MS.gene24428:CDS 40.0%
AATTACGCCTGTTGAAGATG+GGG + chr1.1:3939360-3939379 MS.gene24428:CDS 40.0%
AATTTGCCCAACAAGAGGAT+CGG - chr1.1:3937687-3937706 None:intergenic 40.0%
ACCAACTTCTCAATCACTTC+CGG - chr1.1:3939559-3939578 None:intergenic 40.0%
ACGTTGGTGCAAGAAAGTAA+AGG + chr1.1:3938787-3938806 MS.gene24428:intron 40.0%
AGGAATCTCCTTCACAAGTT+GGG + chr1.1:3938534-3938553 MS.gene24428:CDS 40.0%
AGGATGATATCTTTGCTTGG+AGG - chr1.1:3938577-3938596 None:intergenic 40.0%
ATAACTCTGCATTGAGACTG+GGG - chr1.1:3937653-3937672 None:intergenic 40.0%
ATATCATCCTTGTTGCCTCA+AGG + chr1.1:3938587-3938606 MS.gene24428:CDS 40.0%
CAATTACGCCTGTTGAAGAT+GGG + chr1.1:3939359-3939378 MS.gene24428:CDS 40.0%
CATCTTCAACAGGCGTAATT+GGG - chr1.1:3939360-3939379 None:intergenic 40.0%
CCGAAATACCAGTTATTAGC+TGG + chr1.1:3938687-3938706 MS.gene24428:CDS 40.0%
CGTCAACTATGTCTACTCAA+TGG + chr1.1:3939746-3939765 MS.gene24428:CDS 40.0%
CTATAACTCTGCATTGAGAC+TGG - chr1.1:3937655-3937674 None:intergenic 40.0%
GAATTCAACCCAACTTGTGA+AGG - chr1.1:3938545-3938564 None:intergenic 40.0%
GACGATTCCCATTACATTGA+TGG + chr1.1:3939319-3939338 MS.gene24428:CDS 40.0%
GCACAGTATGCACAAAATAC+CGG + chr1.1:3939601-3939620 MS.gene24428:CDS 40.0%
GGTCTTTGTTCTCTAACACT+GGG + chr1.1:3937523-3937542 MS.gene24428:intron 40.0%
GGTGGTCTCAACTTGATTAA+AGG - chr1.1:3939662-3939681 None:intergenic 40.0%
TAGCTGGATTAAACCACCTA+AGG + chr1.1:3938703-3938722 MS.gene24428:CDS 40.0%
TATTGATGCTACTCCTGTTG+AGG + chr1.1:3939795-3939814 MS.gene24428:CDS 40.0%
TCATCGGGGTATGTATTTCT+AGG + chr1.1:3938929-3938948 MS.gene24428:intron 40.0%
TCGTCAACATCCACTGAAAT+AGG - chr1.1:3939304-3939323 None:intergenic 40.0%
TCTTCCTATACTCACACAAC+TGG + chr1.1:3939712-3939731 MS.gene24428:CDS 40.0%
TGATGAGGGATTACCTTGTT+GGG - chr1.1:3940073-3940092 None:intergenic 40.0%
TGGTCTTTGTTCTCTAACAC+TGG + chr1.1:3937522-3937541 MS.gene24428:intron 40.0%
TGTGTGAGTATAGGAAGAAG+TGG - chr1.1:3939710-3939729 None:intergenic 40.0%
TTAATAGTTCCAGGGGATGT+CGG + chr1.1:3940004-3940023 MS.gene24428:CDS 40.0%
TTAGTGAAGAGCAGTGAACA+TGG + chr1.1:3939490-3939509 MS.gene24428:CDS 40.0%
TTTGTTCTCTAACACTGGGA+TGG + chr1.1:3937527-3937546 MS.gene24428:intron 40.0%
! CTTTGTGCTGTGAGTTTTTG+TGG + chr1.1:3938255-3938274 MS.gene24428:intron 40.0%
!! TCGTTAATTTGCCCAACAAG+AGG - chr1.1:3937692-3937711 None:intergenic 40.0%
AAGGTCTCGGGATCTGATTA+GGG + chr1.1:3937557-3937576 MS.gene24428:intron 45.0%
ACCTCCAGTTGTGTGAGTAT+AGG - chr1.1:3939719-3939738 None:intergenic 45.0%
AGACAACACCTGTCATAGTG+AGG + chr1.1:3937483-3937502 MS.gene24428:intron 45.0%
AGAGAAGACTCGTACTTTGG+AGG - chr1.1:3939202-3939221 None:intergenic 45.0%
AGATCGTGCTAGATCTCACA+TGG + chr1.1:3939080-3939099 MS.gene24428:intron 45.0%
AGCATTTGATGAGGATGTGG+TGG - chr1.1:3939680-3939699 None:intergenic 45.0%
AGCTTGATTCAGCAACATGG+AGG + chr1.1:3939850-3939869 MS.gene24428:CDS 45.0%
ATCTCAGCCACTTCCAACAT+GGG + chr1.1:3937351-3937370 MS.gene24428:CDS 45.0%
CAACAAGGTAATCCCTCATC+AGG + chr1.1:3940072-3940091 MS.gene24428:CDS 45.0%
CACTGTTACGACTATGTCGA+TGG + chr1.1:3939429-3939448 MS.gene24428:CDS 45.0%
CCAATTACGCCTGTTGAAGA+TGG + chr1.1:3939358-3939377 MS.gene24428:CDS 45.0%
CCATCTTCAACAGGCGTAAT+TGG - chr1.1:3939361-3939380 None:intergenic 45.0%
CTAGAGATACTTAGAAGGCC+AGG - chr1.1:3937774-3937793 None:intergenic 45.0%
CTGAAATAGGACTGCATCGA+AGG - chr1.1:3939291-3939310 None:intergenic 45.0%
CTGATGAGGGATTACCTTGT+TGG - chr1.1:3940074-3940093 None:intergenic 45.0%
GAACTATGCATCCCTGATGA+GGG - chr1.1:3940087-3940106 None:intergenic 45.0%
GAGGAATCTCCTTCACAAGT+TGG + chr1.1:3938533-3938552 MS.gene24428:CDS 45.0%
GATGAGGGATTACCTTGTTG+GGG - chr1.1:3940072-3940091 None:intergenic 45.0%
GCAAGAGAAGACTCGTACTT+TGG - chr1.1:3939205-3939224 None:intergenic 45.0%
TAGACTCTCAAGTGCATGCA+TGG - chr1.1:3938385-3938404 None:intergenic 45.0%
TCCGGAAGTGATTGAGAAGT+TGG + chr1.1:3939555-3939574 MS.gene24428:CDS 45.0%
TCCTATACTCACACAACTGG+AGG + chr1.1:3939715-3939734 MS.gene24428:CDS 45.0%
TCGATGCAGTCCTATTTCAG+TGG + chr1.1:3939291-3939310 MS.gene24428:intron 45.0%
TGAACTATGCATCCCTGATG+AGG - chr1.1:3940088-3940107 None:intergenic 45.0%
TGACGATTACTGGAGGAGTA+TGG - chr1.1:3939895-3939914 None:intergenic 45.0%
TGCACTCTTTGTGCTTGGTT+AGG + chr1.1:3938312-3938331 MS.gene24428:intron 45.0%
TGTCTACTCAATGGCATCGT+AGG + chr1.1:3939755-3939774 MS.gene24428:CDS 45.0%
TTCCTTGCACTCTTTGTGCT+TGG + chr1.1:3938307-3938326 MS.gene24428:intron 45.0%
TTTGTTGAAGGGACCTCAAC+AGG - chr1.1:3939811-3939830 None:intergenic 45.0%
! AACCAAGCACAAAGAGTGCA+AGG - chr1.1:3938312-3938331 None:intergenic 45.0%
! AGACTCGTACTTTGGAGGAA+TGG - chr1.1:3939197-3939216 None:intergenic 45.0%
! AGCACAAAGAGTGCAAGGAA+AGG - chr1.1:3938307-3938326 None:intergenic 45.0%
! TTGCAGGTTCTGACGATTAC+TGG - chr1.1:3939905-3939924 None:intergenic 45.0%
!! ATTTTCTGGAGATGTACCGC+TGG + chr1.1:3937393-3937412 MS.gene24428:CDS 45.0%
!! TTTATTTATATATATTATCT+TGG + chr1.1:3938337-3938356 MS.gene24428:intron 5.0%
ACTCTCAAGTGCATGCATGG+TGG - chr1.1:3938382-3938401 None:intergenic 50.0%
AGATCTCACATGGCAAGTGG+TGG + chr1.1:3939090-3939109 MS.gene24428:CDS 50.0%
AGCTTCTCCTTGAGGCAACA+AGG - chr1.1:3938597-3938616 None:intergenic 50.0%
CCTGTTGAAGATGGGGATGT+GGG + chr1.1:3939367-3939386 MS.gene24428:CDS 50.0%
GAAGGTCTCGGGATCTGATT+AGG + chr1.1:3937556-3937575 MS.gene24428:intron 50.0%
GATACTTCTGCAAGCACCAG+CGG - chr1.1:3937412-3937431 None:intergenic 50.0%
GATCTCAGCCACTTCCAACA+TGG + chr1.1:3937350-3937369 MS.gene24428:CDS 50.0%
GCATCATTTCAGCCCCAACA+AGG + chr1.1:3940057-3940076 MS.gene24428:CDS 50.0%
GCATGAGTTCCCTCAAATCC+AGG - chr1.1:3938641-3938660 None:intergenic 50.0%
GCTAGATCTCACATGGCAAG+TGG + chr1.1:3939087-3939106 MS.gene24428:CDS 50.0%
GGAGCGGATACATTATCTCC+TGG + chr1.1:3939388-3939407 MS.gene24428:CDS 50.0%
GGAGGAATGGAAGATATCCG+TGG - chr1.1:3939184-3939203 None:intergenic 50.0%
TCACACAACTGGAGGTCAGA+TGG + chr1.1:3939723-3939742 MS.gene24428:CDS 50.0%
TCAGAACCAGCTTCTCCTTG+AGG - chr1.1:3938605-3938624 None:intergenic 50.0%
TCTCTAACACTGGGATGGAG+AGG + chr1.1:3937532-3937551 MS.gene24428:intron 50.0%
TGAGTTCCCTCAAATCCAGG+AGG - chr1.1:3938638-3938657 None:intergenic 50.0%
TTGTAGACTAGGGGAGACAC+TGG - chr1.1:3937715-3937734 None:intergenic 50.0%
TTGTTGCCTCAAGGAGAAGC+TGG + chr1.1:3938596-3938615 MS.gene24428:CDS 50.0%
! CAGGTTCTGACGATTACTGG+AGG - chr1.1:3939902-3939921 None:intergenic 50.0%
! GCTGGTGCTTGCAGAAGTAT+CGG + chr1.1:3937411-3937430 MS.gene24428:CDS 50.0%
ACACTGGGATGGAGAGGTGA+AGG + chr1.1:3937538-3937557 MS.gene24428:intron 55.0%
AGAAGGGCCGATCCTCTTGT+TGG + chr1.1:3937677-3937696 MS.gene24428:intron 55.0%
AGTTCCAGGGGATGTCGGAA+TGG + chr1.1:3940009-3940028 MS.gene24428:CDS 55.0%
CAGCCACTTCCAACATGGGA+GGG + chr1.1:3937355-3937374 MS.gene24428:CDS 55.0%
CCCACATCCCCATCTTCAAC+AGG - chr1.1:3939370-3939389 None:intergenic 55.0%
GAAGGGCCGATCCTCTTGTT+GGG + chr1.1:3937678-3937697 MS.gene24428:intron 55.0%
GATGGAGAGGTGAAGGTCTC+GGG + chr1.1:3937545-3937564 MS.gene24428:intron 55.0%
GCCTGTTGAAGATGGGGATG+TGG + chr1.1:3939366-3939385 MS.gene24428:CDS 55.0%
GGATGGAGAGGTGAAGGTCT+CGG + chr1.1:3937544-3937563 MS.gene24428:intron 55.0%
TCAGCCACTTCCAACATGGG+AGG + chr1.1:3937354-3937373 MS.gene24428:CDS 55.0%
TGAAGATGGGGATGTGGGAG+CGG + chr1.1:3939372-3939391 MS.gene24428:CDS 55.0%
!! GGTGCTTGCAGAAGTATCGG+AGG + chr1.1:3937414-3937433 MS.gene24428:CDS 55.0%
AGCTCCATTCCGACATCCCC+TGG - chr1.1:3940016-3940035 None:intergenic 60.0%
CTCACATGGCAAGTGGTGGC+TGG + chr1.1:3939094-3939113 MS.gene24428:CDS 60.0%
TGGCCCTCCCATGTTGGAAG+TGG - chr1.1:3937361-3937380 None:intergenic 60.0%
!! CTGACTTGGCCCTCCCATGT+TGG - chr1.1:3937367-3937386 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 3937292 3940143 3937292 ID=MS.gene24428
chr1.1 mRNA 3937292 3940143 3937292 ID=MS.gene24428.t1;Parent=MS.gene24428
chr1.1 exon 3937292 3937435 3937292 ID=MS.gene24428.t1.exon1;Parent=MS.gene24428.t1
chr1.1 CDS 3937292 3937435 3937292 ID=cds.MS.gene24428.t1;Parent=MS.gene24428.t1
chr1.1 exon 3938515 3938724 3938515 ID=MS.gene24428.t1.exon2;Parent=MS.gene24428.t1
chr1.1 CDS 3938515 3938724 3938515 ID=cds.MS.gene24428.t1;Parent=MS.gene24428.t1
chr1.1 exon 3939082 3939206 3939082 ID=MS.gene24428.t1.exon3;Parent=MS.gene24428.t1
chr1.1 CDS 3939082 3939206 3939082 ID=cds.MS.gene24428.t1;Parent=MS.gene24428.t1
chr1.1 exon 3939300 3940143 3939300 ID=MS.gene24428.t1.exon4;Parent=MS.gene24428.t1
chr1.1 CDS 3939300 3940143 3939300 ID=cds.MS.gene24428.t1;Parent=MS.gene24428.t1
Gene Sequence

>MS.gene24428

ATGTTGTCTCTCTCTAAAATTCTGTTCTTTTTCTGTTTCAAAAGGAGACTGAGTGCAAGATCTCAGCCACTTCCAACATGGGAGGGCCAAGTCAGTGTGATATTTTCTGGAGATGTACCGCTGGTGCTTGCAGAAGTATCGGAGGTAAAGTTGACAGAAATTCTTGGTTGGTCAGCCAATTAATTCACTTCAGACAACACCTGTCATAGTGAGGCTATATAAGTTGCAATTGGTCTTTGTTCTCTAACACTGGGATGGAGAGGTGAAGGTCTCGGGATCTGATTAGGGCATTTTGAAAAAGCTTGCAATTTGGATTTAAGAATCTGTTTTCACTTTTAATCAGGATCTTAACTTGGTGCCCCAGTCTCAATGCAGAGTTATAGAGAGAAGGGCCGATCCTCTTGTTGGGCAAATTAACGACCAGTGTCTCCCCTAGTCTACAATATTCATAAAAATATGAGTAGTAGAAGATATTGATTCCTGGCCTTCTAAGTATCTCTAGTATGAATCTTATGGATGGAAAATTGAGTATCATCAAGTCCTTTATTATGATTACCCATAATAATTTGAATAAGAAAGAGTTTGTTATATGATATGAATTATGAAATATCGCTGAAGTATTTATTGTGCATTCAAAATGGCTATAAATATTCTGGCTATGTCTCGTTGTGTTGTTTTCTAGAGATAACAATTTACATTCAATCATCAAATATTGTGCTTGTATGAAATCTATCACAAATCGCTTAATCTATTCCACATATATTATTAATATTATCAAAAGTTTAAGTGATCATGATAGCAGTTTGATGCAAGTTTATTTTGTGTGCCATCTTTTACTGTCATATTTTAGCTGTATTTGATTTTTCTCTATTACTTATGATTTGTTATCAATAAGCTTGTGTTTCTCTGTTTCAAGTGTTTGCAAATTAGTAATCGCCACTTTCATCCAAAACCTGTTTATTTCTTTGTGCTGTGAGTTTTTGTGGTTTTGTACTACACATTATTTTAGTGTCCTTTCCTTGCACTCTTTGTGCTTGGTTAGGCGTTTATTTATATATATTATCTTGGCTTATTAAAAAAAAAGTAACCACCATGCATGCACTTGAGAGTCTATTGAGTGAACAGAATCAATAATTTAAGATGATGTTGCCCAGATGTTCATTATAAAGTACATATTATAAGAGTGCTAAAAGGAAAAGGACTGTTTCCTTTTTGTTGAACAGGTTAGATTATTCTTATCTGAGGAATCTCCTTCACAAGTTGGGTTGAATTCTCAAGATCACCTCCAAGCAAAGATATCATCCTTGTTGCCTCAAGGAGAAGCTGGTTCTGATGATATTTTACCTCCTGGATTTGAGGGAACTCATGCTTCAAGTCAGTTTGACATTAAGCCGTCCGAAATACCAGTTATTAGCTGGATTAAACCACCTAAGGTATTTTTGTAGTTATATTTTAAACAATATCCATAGTAATCTTCAGTAATATTTATATTTCAACGTTGGTGCAAGAAAGTAAAGGAAAAAAAGAAATGATATTTGAACATCTATTTTGCATCAACTATTTTGTCAAACCTATGTATATAGCGGTTTTTTCGTAAAAGCAAAAGAAAAAAAGCTTCAAAACAGTATGCCAATATATCATCGGGGTATGTATTTCTAGGTTGTTGGGAAATTTGTCAAAAAAGTGATGTCAATATATCATAACTCGAAAAAAAAAGAAGGTGAAATGTGAGTTTGCTTGTACTATTTTATTTTAATTCTTCATTATTTGTAATTGTGCGATTCTGTCAGATCGTGCTAGATCTCACATGGCAAGTGGTGGCTGGAGAAGAAAGCAAAGAGATATTTGATCAACATCAGAGGGAGATGAGAGTACTTGAAGCTATTTATCCACGGATATCTTCCATTCCTCCAAAGTACGAGTCTTCTCTTGCCTTGTTTGATATTTCTTTTTTTAGTTTTTGCACGATTTTTTTAATAAAAGATTTTTTTTTCTTCCTTCGATGCAGTCCTATTTCAGTGGATGTTGACGATTCCCATTACATTGATGGTCATGTTGTCGTGATCCCAATTACGCCTGTTGAAGATGGGGATGTGGGAGCGGATACATTATCTCCTGGTATATTGGATGGTTCAAACACTGTTACGACTATGTCGATGGTGTCTGATGTAGCTGCTGCATCTGTTGCTTTAACTAGCTTAGTGAAGAGCAGTGAACATGGTAATTTGATTGATCCTGAATTACTCAATAATATTCTCAACAATCCGGAAGTGATTGAGAAGTTGGTTAGAAATTATAATGTTCAAAATGCACAGTATGCACAAAATACCGGATCATCATCAAATGTTTTTTCACATCCTCCTAATCCCTTTAATCAAGTTGAGACCACCACATCCTCATCAAATGCTTTTTCATCCACTTCTTCCTATACTCACACAACTGGAGGTCAGATGGCGTCAACTATGTCTACTCAATGGCATCGTAGGCCTGAAATCAGTTCAGCTATTGATGCTACTCCTGTTGAGGTCCCTTCAACAAAAGATGTAAACTACTATAAGAGCTTGATTCAGCAACATGGAGGAGATAAACAAGAAGCACTTCCATACTCCTCCAGTAATCGTCAGAACCTGCAACCATTATCAAACTATGATACAGCACACAATTTTAGAGCAAAAGGATCAAAACCAAAAACAACGAAGCCTTGTATCTTTTTTAATAGTTCCAGGGGATGTCGGAATGGAGCTAACTGTACCTTTCAGCATGATGCATCATTTCAGCCCCAACAAGGTAATCCCTCATCAGGGATGCATAGTTCAAAGAGAATGAAAATGGATAATGAGATCAGCAGTTAA
Protein sequence

>MS.gene24428.t1

MLSLSKILFFFCFKRRLSARSQPLPTWEGQVSVIFSGDVPLVLAEVSEVRLFLSEESPSQVGLNSQDHLQAKISSLLPQGEAGSDDILPPGFEGTHASSQFDIKPSEIPVISWIKPPKIVLDLTWQVVAGEESKEIFDQHQREMRVLEAIYPRISSIPPNPISVDVDDSHYIDGHVVVIPITPVEDGDVGADTLSPGILDGSNTVTTMSMVSDVAAASVALTSLVKSSEHGNLIDPELLNNILNNPEVIEKLVRNYNVQNAQYAQNTGSSSNVFSHPPNPFNQVETTTSSSNAFSSTSSYTHTTGGQMASTMSTQWHRRPEISSAIDATPVEVPSTKDVNYYKSLIQQHGGDKQEALPYSSSNRQNLQPLSNYDTAHNFRAKGSKPKTTKPCIFFNSSRGCRNGANCTFQHDASFQPQQGNPSSGMHSSKRMKMDNEISS