AlfalfaGEDB | MS.gene24530

M. sativa cultivar XinJiangDaYe / MS.gene24530

KEGG: K09422
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24530.t1	XP_013465930.1	93.5	168	10	1	20	187	86	252	8.40E-82	313.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24530.t1	F4JSU0	70.6	34	10	0	20	53	86	119	8.2e-09	62.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24530.t1	A0A072VEE8	93.5	168	10	1	20	187	86	252	6.1e-82	313.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene24530.t1	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24530.t1	MTR_1g017000	93.452	168	10	1	20	187	86	252	6.34e-111	317
MS.gene24530.t1	MTR_6g009430	50.725	69	30	3	20	85	85	152	1.71e-12	65.1
MS.gene24530.t1	MTR_3g103570	41.096	73	43	0	20	92	86	158	1.99e-12	64.7
MS.gene24530.t1	MTR_7g102110	56.452	62	20	2	20	74	86	147	2.80e-12	63.9
MS.gene24530.t1	MTR_4g057635	70.588	34	10	0	20	53	86	119	2.04e-11	61.6
MS.gene24530.t1	MTR_6g090405	62.500	40	15	0	15	54	81	120	2.06e-11	61.2
MS.gene24530.t1	MTR_1g017140	67.647	34	11	0	20	53	86	119	5.14e-11	60.8
MS.gene24530.t1	MTR_5g014990	67.647	34	11	0	20	53	86	119	5.26e-11	60.5
MS.gene24530.t1	MTR_4g091490	53.333	45	19	1	20	64	86	128	5.85e-11	60.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24530.t1	AT5G16770	38.136	118	60	6	20	134	85	192	1.34e-12	65.1
MS.gene24530.t1	AT5G16770	38.136	118	60	6	20	134	85	192	1.34e-12	65.1
MS.gene24530.t1	AT5G16770	38.136	118	60	6	20	134	85	192	2.20e-12	64.7
MS.gene24530.t1	AT5G16770	38.136	118	60	6	20	134	85	192	2.20e-12	64.7
MS.gene24530.t1	AT5G57620	53.846	52	21	1	20	71	86	134	1.65e-11	62.0
MS.gene24530.t1	AT4G37780	70.588	34	10	0	20	53	86	119	2.21e-11	61.6
MS.gene24530.t1	AT3G23250	45.000	80	41	2	15	92	23	101	3.65e-11	60.5
MS.gene24530.t1	AT5G23000	63.158	38	14	0	15	52	81	118	8.09e-11	60.1
MS.gene24530.t1	AT3G23250	45.000	80	41	2	15	92	80	158	9.21e-11	59.7
MS.gene24530.t1	AT5G65790	69.697	33	10	0	20	52	86	118	9.63e-11	60.1

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Find 29 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGAAGAAGAAGATTT+TGG	0.243833	1.2:+5824418	MS.gene24530:CDS
ACATCATTGTCTGTTCTGCC+AGG	0.476737	1.2:-5824377	None:intergenic
ACTTTATTGTCTAGCATAAT+TGG	0.238985	1.2:-5824584	None:intergenic
AGAATAGAGGGTGAAAATGA+TGG	0.524462	1.2:-5823419	None:intergenic
AGCATAATTGGAAGTGGTGA+AGG	0.515734	1.2:-5824572	None:intergenic
ATAATTGGAAGTGGTGAAGG+TGG	0.597724	1.2:-5824569	None:intergenic
ATCTTAACTGCTTTCCTGAG+TGG	0.564962	1.2:+5824789	MS.gene24530:CDS
ATGGAATCATTGATGGATTT+TGG	0.132955	1.2:+5824734	MS.gene24530:CDS
ATTGCAATTCAAGATCAAGC+TGG	0.635582	1.2:+5824701	MS.gene24530:CDS
CAGCAATATTATTAATCCTT+TGG	0.356887	1.2:+5824652	MS.gene24530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAGATTAAGACCCAAAAAA+TGG	+	chr1.2:5824093-5824112	MS.gene24530:intron	30.0%
GAAATTATAACAACCAACTG+GGG	+	chr1.2:5823735-5823754	MS.gene24530:intron	30.0%
GTACTATGAAACTAACTTTG+AGG	-	chr1.2:5824272-5824291	None:intergenic	30.0%
TAATTTCTCACTTTGACTGA+AGG	-	chr1.2:5823893-5823912	None:intergenic	30.0%
TAGTTTCATAGTACTTCAAG+AGG	+	chr1.2:5824278-5824297	MS.gene24530:intron	30.0%
TGAATCTATGGAATCATTGA+TGG	+	chr1.2:5824727-5824746	MS.gene24530:CDS	30.0%
TGAGAACTATTCAAACTTTG+AGG	-	chr1.2:5824310-5824329	None:intergenic	30.0%
AGAATAGAGGGTGAAAATGA+TGG	-	chr1.2:5823422-5823441	None:intergenic	35.0%
ATTGCAATTCAAGATCAAGC+TGG	+	chr1.2:5824701-5824720	MS.gene24530:CDS	35.0%
CACTTAGACCAACATTATGT+CGG	-	chr1.2:5823927-5823946	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	5823392	5824847	5823392	ID=MS.gene24530
chr1.2	mRNA	5823392	5824847	5823392	ID=MS.gene24530.t1;Parent=MS.gene24530
chr1.2	exon	5823392	5823459	5823392	ID=MS.gene24530.t1.exon1;Parent=MS.gene24530.t1
chr1.2	CDS	5823392	5823459	5823392	ID=cds.MS.gene24530.t1;Parent=MS.gene24530.t1
chr1.2	exon	5824352	5824847	5824352	ID=MS.gene24530.t1.exon2;Parent=MS.gene24530.t1
chr1.2	CDS	5824352	5824847	5824352	ID=cds.MS.gene24530.t1;Parent=MS.gene24530.t1

Gene Sequence

>MS.gene24530

ATGGAGGCTTCACTGAAGAAGAGGATGCCATCATTTTCACCCTCTATTCTCAAATGGGTAGCAGGCAAGTACTATTTCCAACTTATTTAATTAAGTTGTATCTATTTTCACTATATAATTTCCAAATTGTTATTATTATAAACGTTGATCATTGTTAACTGTTATAATCACTTTATTTAATTTAGTATTATATATAGTTTTTCATAAGAGTTGATCATTGTTAAGTGCTAACTATTACAACTTTATTTAATTTAGTACTATGTATTGTTTTTCTTTAAGAGTTGATGATTTTTAATGTGTACAAGTTGGAAAGATTTGTTTTTTCATATAGATAATAAATTAAGAAATTATAACAACCAACTGGGGTTGAGTCAACTTGAAGGGGATAACTTCCCATGATGTTATAAACTAGAGTTCGAATTCCGAGACTAAAATTTAAATTTCAGTTGGGAGAGATGTCGTATTTAGAGTTTGAGGGTGTTTTAAATAAAATTATAACCTTCAGTCAAAGTGAGAAATTATAACCGACAGTCCGACATAATGTTGGTCTAAGTGTAACTGGATTGGACTCAAATTTTTTTAAGCAATGTCTCATGTTCAAACTTTATCAACAGAAAAAATGTGATTAGAAGGAGAGAAACTACTAAATGTTGGATTTTATTTAATTATCTGTCGAACCTGTGATGATTATGGTCTTTCCCCAAGATTAAGACCCAAAAAATGGTTATAGAATATAGATGCTTGATACCAATAACTTGATTTTAAAAAAAGTGAGAAATTATGGATTGATATTCTATCATTTAATTATTCATATATACAAAATAAATGTACTATCTATCTATTAGTAGTATTGATTTTTTATAGTCAGTTGAATGGTCCTCAAAGTTAGTTTCATAGTACTTCAAGAGGAATATGCCTCAAAGTTTGAATAGTTCTCATGAAATTTGTGATTTTGTGCAGATGGTCTGCCATAGCATCTAAACTACCTGGCAGAACAGACAATGATGTGAAAAACTATTGGAACACAAAACTGAAGAAGAAGATTTTGGCAGGAATAGTTAAGCTCAAACCATTGACTGAAAATGACAATACTATCCCTTCAACTCCATCACTAACTCAAAGTTCCAACCCTCAAAATTCAGAAATCAATTTCCCTGCTTCACAAAATCAACATTCTCCACCTTCACCACTTCCAATTATGCTAGACAATAAAGTTGACAAAATTCATGATGCTGTAGTATCAGAAATTGGTACAAGTAACAGCAATATTATTAATCCTTTGGTGTCAATTTCTCAAGACTCTTCAAGCATTGCAATTCAAGATCAAGCTGGTGATGAATCTATGGAATCATTGATGGATTTTGGCTTTGGATTTCCTCATGATGATCATGTCAATGATCTTAACTGCTTTCCTGAGTGGGTTGATTTTAGTTATGCTGACATTAAGCCAAATTGA

Protein sequence

>MS.gene24530.t1

MEASLKKRMPSFSPSILKWVAGKWSAIASKLPGRTDNDVKNYWNTKLKKKILAGIVKLKPLTENDNTIPSTPSLTQSSNPQNSEINFPASQNQHSPPSPLPIMLDNKVDKIHDAVVSEIGTSNSNIINPLVSISQDSSSIAIQDQAGDESMESLMDFGFGFPHDDHVNDLNCFPEWVDFSYADIKPN