AlfalfaGEDB | MS.gene24568

M. sativa cultivar XinJiangDaYe / MS.gene24568

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0008270 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043167 GO:0043169 GO:0043565 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24568.t1	PON56353.1	60.7	61	21	2	4	61	55	115	2.10E-09	71.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24568.t1	Q6YW48	81.8	33	6	0	29	61	177	209	5.5e-11	68.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24568.t1	A0A2P5C5N5	60.7	61	21	2	4	61	55	115	1.5e-09	71.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene24568.t1	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24568.t1	MTR_1g016170	52.703	74	27	2	2	72	166	234	2.05e-17	74.7
MS.gene24568.t1	MTR_1g016200	55.738	61	24	1	12	72	47	104	2.37e-15	65.5
MS.gene24568.t1	MTR_7g112330	44.828	58	32	0	29	86	23	80	5.47e-14	62.8
MS.gene24568.t1	MTR_4g121110	54.167	48	22	0	30	77	32	79	6.12e-14	63.2
MS.gene24568.t1	MTR_2g038720	81.250	32	6	0	26	57	162	193	1.78e-13	63.9
MS.gene24568.t1	MTR_4g126350	81.818	33	6	0	25	57	183	215	1.14e-12	62.0
MS.gene24568.t1	MTR_5g020230	63.636	33	12	0	30	62	124	156	2.60e-11	57.4
MS.gene24568.t1	MTR_1g094930	67.742	31	10	0	30	60	149	179	8.17e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24568.t1	AT5G56860	54.098	61	25	1	4	61	203	263	5.64e-15	68.6
MS.gene24568.t1	AT5G49300	51.852	54	21	1	14	62	17	70	9.17e-15	65.1
MS.gene24568.t1	AT5G26930	50.769	65	25	2	30	87	28	92	6.07e-14	62.4
MS.gene24568.t1	AT3G16870	50.000	62	30	1	26	87	40	100	3.14e-13	62.0
MS.gene24568.t1	AT4G26150	80.645	31	6	0	29	59	200	230	3.48e-13	63.5
MS.gene24568.t1	AT2G18380	46.552	58	26	1	9	66	78	130	6.30e-13	61.6
MS.gene24568.t1	AT4G16141	62.500	40	15	0	30	69	39	78	7.35e-13	61.2
MS.gene24568.t1	AT3G06740	69.697	33	10	0	30	62	43	75	1.90e-12	59.3
MS.gene24568.t1	AT4G36620	57.895	38	16	0	30	67	77	114	4.50e-12	59.3
MS.gene24568.t1	AT3G50870	63.636	33	12	0	30	62	154	186	3.17e-11	57.8
MS.gene24568.t1	AT1G08010	70.968	31	9	0	30	60	222	252	9.15e-11	56.6
MS.gene24568.t1	AT1G08010	70.968	31	9	0	30	60	222	252	9.15e-11	56.6
MS.gene24568.t1	AT1G08010	70.968	31	9	0	30	60	222	252	9.15e-11	56.6

Find 28 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCAAAAGCTGCTAAAGTTT+TGG	0.274463	1.2:+6307721	MS.gene24568:CDS
CCTTTGGAGGAGTGGACCTA+TGG	0.393594	1.2:+6307252	MS.gene24568:CDS
CCTTAAACGGAGAAGGAAAA+TGG	0.403851	1.2:+6307700	MS.gene24568:CDS
GCAACGCATGTGGCCTTAAA+CGG	0.433689	1.2:+6307687	MS.gene24568:CDS
TTTGGATACTCACAAGGAAA+AGG	0.441775	1.2:+6307739	MS.gene24568:CDS
ACTCCTCCAAAGGGGAGTCT+TGG	0.473678	1.2:-6307242	None:intergenic
GTGTTAAATACTCTAGCCTT+CGG	0.474400	1.2:-6307172	None:intergenic
ACTATAGTGGTTGTAGCAGC+TGG	0.486129	1.2:-6307199	None:intergenic
TTGGATACTCACAAGGAAAA+GGG	0.490862	1.2:+6307740	MS.gene24568:CDS
ACAATCTGAGCAAACTATAG+TGG	0.494689	1.2:-6307212	None:intergenic
CTTTGGAGGAGTGGACCTAT+GGG	0.500407	1.2:+6307253	MS.gene24568:CDS
TACTCTAGCCTTCGGATATG+TGG	0.506608	1.2:-6307164	None:intergenic
CAACCAAGACTCCCCTTTGG+AGG	0.510410	1.2:+6307239	MS.gene24568:CDS
AGACAACCAAGACTCCCCTT+TGG	0.517499	1.2:+6307236	MS.gene24568:CDS
CATAGGTCCACTCCTCCAAA+GGG	0.525622	1.2:-6307251	None:intergenic
GAGTGGACCTATGGGTCCTA+AGG	0.528357	1.2:+6307261	MS.gene24568:CDS
CTAATTACCTTAGGACCCAT+AGG	0.530405	1.2:-6307268	None:intergenic
TGGCGAAAGCTCATCATATG+AGG	0.534699	1.2:+6307766	MS.gene24568:CDS
CCATAGGTCCACTCCTCCAA+AGG	0.542869	1.2:-6307252	None:intergenic
AAGACTCCCCTTTGGAGGAG+TGG	0.565345	1.2:+6307244	MS.gene24568:CDS
AGCCTTCGGATATGTGGTGG+TGG	0.569867	1.2:-6307158	None:intergenic
AGTGGTTGTAGCAGCTGGAA+TGG	0.582766	1.2:-6307194	None:intergenic
CATGTGGCCTTAAACGGAGA+AGG	0.606403	1.2:+6307693	MS.gene24568:CDS
ACTCACAAGGAAAAGGGCAA+TGG	0.626006	1.2:+6307746	MS.gene24568:CDS
CAGTCTTTGTGCAACGCATG+TGG	0.630452	1.2:+6307677	MS.gene24568:intron
TGCCACCACCACATATCCGA+AGG	0.641370	1.2:+6307156	MS.gene24568:CDS
TCTAGCCTTCGGATATGTGG+TGG	0.647590	1.2:-6307161	None:intergenic
ATAGGTCCACTCCTCCAAAG+GGG	0.735481	1.2:-6307250	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTAGTTATGTATATAGTTA+AGG	+	chr1.2:6307357-6307376	MS.gene24568:intron	20.0%
!	ACATGTTTCATGTTAACTAT+AGG	-	chr1.2:6307652-6307671	None:intergenic	25.0%
!	ATAGTTAACATGAAACATGT+TGG	+	chr1.2:6307652-6307671	MS.gene24568:intron	25.0%
!	GTTACAAATGTGAAACTTAA+AGG	+	chr1.2:6307608-6307627	MS.gene24568:intron	25.0%
!!	ACTGTTTTCATTTACTATCA+CGG	+	chr1.2:6307321-6307340	MS.gene24568:intron	25.0%
!!	ATAGATCCTTTAAGTTTCAA+AGG	-	chr1.2:6307586-6307605	None:intergenic	25.0%
	AAAACAGTAGCGAATAAGTT+GGG	-	chr1.2:6307309-6307328	None:intergenic	30.0%
	AAGTTTCAAAGGTTACAGAT+AGG	+	chr1.2:6307434-6307453	MS.gene24568:intron	30.0%
	AAGTTTCAAAGGTTACAGAT+AGG	-	chr1.2:6307453-6307434	None:intergenic	30.0%
	AGAGACTATCTAATTACCTT+AGG	-	chr1.2:6307280-6307299	None:intergenic	30.0%
	CTGAAACCTTAGAAACTTAA+AGG	+	chr1.2:6307548-6307567	MS.gene24568:intron	30.0%
	CTGAAACCTTAGAAACTTAA+AGG	-	chr1.2:6307567-6307548	None:intergenic	30.0%
	GTTACAAACGTGAAACTTAA+AGG	-	chr1.2:6307401-6307420	None:intergenic	30.0%
!	ATAGGTCCTTTAAGTTTCAA+AGG	+	chr1.2:6307423-6307442	MS.gene24568:intron	30.0%
!	ATAGGTCCTTTAAGTTTCTA+AGG	+	chr1.2:6307452-6307471	MS.gene24568:intron	30.0%
!	ATAGGTCCTTTAAGTTTCTA+AGG	-	chr1.2:6307471-6307452	None:intergenic	30.0%
!!	AAGTTTCTAAGGTTTCAGAT+AGG	+	chr1.2:6307463-6307482	MS.gene24568:intron	30.0%
!!	AAGTTTCTAAGGTTTCAGAT+AGG	-	chr1.2:6307482-6307463	None:intergenic	30.0%
!!	CTGTAACCTTTGAAACTTAA+AGG	+	chr1.2:6307577-6307596	MS.gene24568:intron	30.0%
!!	CTGTAACCTTTGAAACTTAA+AGG	-	chr1.2:6307596-6307577	None:intergenic	30.0%
!!!	TAAAGTTTTGGATACTCACA+AGG	+	chr1.2:6307733-6307752	MS.gene24568:CDS	30.0%
	ACAATCTGAGCAAACTATAG+TGG	-	chr1.2:6307215-6307234	None:intergenic	35.0%
	GAAAACAGTAGCGAATAAGT+TGG	-	chr1.2:6307310-6307329	None:intergenic	35.0%
	GAAACCAACTCGAAACTTAA+AGG	-	chr1.2:6307490-6307509	None:intergenic	35.0%
	GTTTCACGTTTGTAACAGAT+AGG	+	chr1.2:6307405-6307424	MS.gene24568:intron	35.0%
	TTGGATACTCACAAGGAAAA+GGG	+	chr1.2:6307740-6307759	MS.gene24568:CDS	35.0%
	TTTGGATACTCACAAGGAAA+AGG	+	chr1.2:6307739-6307758	MS.gene24568:CDS	35.0%
!	GTGTTAAATACTCTAGCCTT+CGG	-	chr1.2:6307175-6307194	None:intergenic	35.0%
	ATGTGAAACTTAAAGGACCC+AGG	+	chr1.2:6307615-6307634	MS.gene24568:intron	40.0%
	CCTTAAACGGAGAAGGAAAA+TGG	+	chr1.2:6307700-6307719	MS.gene24568:CDS	40.0%
	CTAATTACCTTAGGACCCAT+AGG	-	chr1.2:6307271-6307290	None:intergenic	40.0%
	CTTTCTATCAACCTCCGTTA+AGG	+	chr1.2:6307519-6307538	MS.gene24568:intron	40.0%
	GGTTTCAGATAGGTCCTTAA+CGG	-	chr1.2:6307536-6307555	None:intergenic	40.0%
	TGTGAAACTTAAAGGACCCA+GGG	+	chr1.2:6307616-6307635	MS.gene24568:intron	40.0%
!	AGGTCCTTTAAGTTTCGAGT+TGG	+	chr1.2:6307483-6307502	MS.gene24568:intron	40.0%
!	CCATTTTCCTTCTCCGTTTA+AGG	-	chr1.2:6307703-6307722	None:intergenic	40.0%
!	GGCAAAAGCTGCTAAAGTTT+TGG	+	chr1.2:6307721-6307740	MS.gene24568:CDS	40.0%
!!	CTTAACGGAGGTTGATAGAA+AGG	-	chr1.2:6307521-6307540	None:intergenic	40.0%
!!	GTTTCGAGTTGGTTTCAGAT+AGG	+	chr1.2:6307494-6307513	MS.gene24568:intron	40.0%
	ACGTGAAACTTAAAGGACCC+AGG	-	chr1.2:6307394-6307413	None:intergenic	45.0%
	ACTATAGTGGTTGTAGCAGC+TGG	-	chr1.2:6307202-6307221	None:intergenic	45.0%
	ACTCACAAGGAAAAGGGCAA+TGG	+	chr1.2:6307746-6307765	MS.gene24568:CDS	45.0%
	CGTGAAACTTAAAGGACCCA+GGG	-	chr1.2:6307393-6307412	None:intergenic	45.0%
	TATAGGCCAAATACCTCCCT+GGG	-	chr1.2:6307635-6307654	None:intergenic	45.0%
	TGGCGAAAGCTCATCATATG+AGG	+	chr1.2:6307766-6307785	MS.gene24568:CDS	45.0%
	TTAAGGCCAAATACCTCCCT+GGG	+	chr1.2:6307374-6307393	MS.gene24568:intron	45.0%
	TTCAGATAGGTCCTTAACGG+AGG	-	chr1.2:6307533-6307552	None:intergenic	45.0%
!	TACTCTAGCCTTCGGATATG+TGG	-	chr1.2:6307167-6307186	None:intergenic	45.0%
	AGACAACCAAGACTCCCCTT+TGG	+	chr1.2:6307236-6307255	MS.gene24568:CDS	50.0%
	AGTGGTTGTAGCAGCTGGAA+TGG	-	chr1.2:6307197-6307216	None:intergenic	50.0%
	ATAGGTCCACTCCTCCAAAG+GGG	-	chr1.2:6307253-6307272	None:intergenic	50.0%
	CAGTCTTTGTGCAACGCATG+TGG	+	chr1.2:6307677-6307696	MS.gene24568:intron	50.0%
	CATAGGTCCACTCCTCCAAA+GGG	-	chr1.2:6307254-6307273	None:intergenic	50.0%
	CTATAGGCCAAATACCTCCC+TGG	-	chr1.2:6307636-6307655	None:intergenic	50.0%
	GAAACTTAAAGGACCCAGGG+AGG	+	chr1.2:6307619-6307638	MS.gene24568:intron	50.0%
	GAAACTTAAAGGACCCAGGG+AGG	-	chr1.2:6307638-6307619	None:intergenic	50.0%
	GTTAAGGCCAAATACCTCCC+TGG	+	chr1.2:6307373-6307392	MS.gene24568:intron	50.0%
!	CATGTGGCCTTAAACGGAGA+AGG	+	chr1.2:6307693-6307712	MS.gene24568:CDS	50.0%
!	CTTTGGAGGAGTGGACCTAT+GGG	+	chr1.2:6307253-6307272	MS.gene24568:CDS	50.0%
!	GCAACGCATGTGGCCTTAAA+CGG	+	chr1.2:6307687-6307706	MS.gene24568:CDS	50.0%
!	TCTAGCCTTCGGATATGTGG+TGG	-	chr1.2:6307164-6307183	None:intergenic	50.0%
!!	AAAGGACCCAGGGAGGTATT+TGG	+	chr1.2:6307626-6307645	MS.gene24568:intron	50.0%
!!	AAAGGACCCAGGGAGGTATT+TGG	-	chr1.2:6307645-6307626	None:intergenic	50.0%
	ACTCCTCCAAAGGGGAGTCT+TGG	-	chr1.2:6307245-6307264	None:intergenic	55.0%
	AGCCTTCGGATATGTGGTGG+TGG	-	chr1.2:6307161-6307180	None:intergenic	55.0%
	CAACCAAGACTCCCCTTTGG+AGG	+	chr1.2:6307239-6307258	MS.gene24568:CDS	55.0%
	CCATAGGTCCACTCCTCCAA+AGG	-	chr1.2:6307255-6307274	None:intergenic	55.0%
	GAGTGGACCTATGGGTCCTA+AGG	+	chr1.2:6307261-6307280	MS.gene24568:CDS	55.0%
	TGCCACCACCACATATCCGA+AGG	+	chr1.2:6307156-6307175	MS.gene24568:CDS	55.0%
!	AAGACTCCCCTTTGGAGGAG+TGG	+	chr1.2:6307244-6307263	MS.gene24568:CDS	55.0%
!	CCTTTGGAGGAGTGGACCTA+TGG	+	chr1.2:6307252-6307271	MS.gene24568:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	6307136	6307805	6307136	ID=MS.gene24568
chr1.2	mRNA	6307136	6307805	6307136	ID=MS.gene24568.t1;Parent=MS.gene24568
chr1.2	exon	6307136	6307282	6307136	ID=MS.gene24568.t1.exon1;Parent=MS.gene24568.t1
chr1.2	CDS	6307136	6307282	6307136	ID=cds.MS.gene24568.t1;Parent=MS.gene24568.t1
chr1.2	exon	6307680	6307805	6307680	ID=MS.gene24568.t1.exon2;Parent=MS.gene24568.t1
chr1.2	CDS	6307680	6307805	6307680	ID=cds.MS.gene24568.t1;Parent=MS.gene24568.t1

Gene Sequence

>MS.gene24568

ATGAGAATGAAGAACACAACTGCCACCACCACATATCCGAAGGCTAGAGTATTTAACACCATTCCAGCTGCTACAACCACTATAGTTTGCTCAGATTGTGAGACAACCAAGACTCCCCTTTGGAGGAGTGGACCTATGGGTCCTAAGGTAATTAGATAGTCTCTCTTCTTCCCAACTTATTCGCTACTGTTTTCATTTACTATCACGGTACACTTTAAATATCTAGTTATGTATATAGTTAAGGCCAAATACCTCCCTGGGTCCTTTAAGTTTCACGTTTGTAACAGATAGGTCCTTTAAGTTTCAAAGGTTACAGATAGGTCCTTTAAGTTTCTAAGGTTTCAGATAGGTCCTTTAAGTTTCGAGTTGGTTTCAGATAGGTCCTTTCTATCAACCTCCGTTAAGGACCTATCTGAAACCTTAGAAACTTAAAGGACCTATCTGTAACCTTTGAAACTTAAAGGATCTATCTGTTACAAATGTGAAACTTAAAGGACCCAGGGAGGTATTTGGCCTATAGTTAACATGAAACATGTTGGTGCAGTCTTTGTGCAACGCATGTGGCCTTAAACGGAGAAGGAAAATGGCAAAAGCTGCTAAAGTTTTGGATACTCACAAGGAAAAGGGCAATGGCGAAAGCTCATCATATGAGGATGTGAAGAAGCTTTAA

Protein sequence

>MS.gene24568.t1

MRMKNTTATTTYPKARVFNTIPAATTTIVCSDCETTKTPLWRSGPMGPKSLCNACGLKRRRKMAKAAKVLDTHKEKGNGESSSYEDVKKL