AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24603

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24603.t1 MTR_1g064430 97.185 675 13 3 1 673 14 684 0.0 1353
MS.gene24603.t1 MTR_1g064430 97.185 675 13 3 1 673 1 671 0.0 1353
MS.gene24603.t1 MTR_2g094570 56.585 691 253 13 4 673 42 706 0.0 732
MS.gene24603.t1 MTR_2g094570 57.652 673 238 13 22 673 4 650 0.0 730
MS.gene24603.t1 MTR_2g094570 57.715 674 238 13 22 674 4 651 0.0 730
MS.gene24603.t1 MTR_4g058930 58.902 674 218 14 26 673 9 649 0.0 714
MS.gene24603.t1 MTR_4g058930 59.104 670 223 13 26 673 9 649 0.0 714
MS.gene24603.t1 MTR_7g101280 54.892 603 239 11 22 611 24 606 0.0 622
MS.gene24603.t1 MTR_0041s0110 54.892 603 239 11 22 611 24 606 0.0 622
MS.gene24603.t1 MTR_1g094960 49.845 646 241 12 1 604 1 605 0.0 567
MS.gene24603.t1 MTR_7g101275 50.000 408 145 5 38 434 1 360 7.69e-125 376
MS.gene24603.t1 MTR_0041s0120 50.000 408 157 7 38 434 1 372 6.57e-122 369
MS.gene24603.t1 MTR_3g073420 41.397 587 289 16 26 586 16 573 1.61e-117 364
MS.gene24603.t1 MTR_5g061220 42.765 463 212 14 24 472 14 437 6.49e-104 327
MS.gene24603.t1 MTR_2g024430 41.422 408 163 11 26 422 17 359 2.57e-89 288
MS.gene24603.t1 MTR_5g040880 38.821 407 185 7 23 424 5 352 4.42e-87 282
MS.gene24603.t1 MTR_5g082140 37.882 425 194 10 26 441 9 372 2.50e-80 263
MS.gene24603.t1 MTR_1g058210 36.190 420 203 10 24 435 7 369 4.71e-80 262
MS.gene24603.t1 MTR_4g021580 30.612 686 384 19 13 673 3 621 5.98e-79 265
MS.gene24603.t1 MTR_4g021580 30.219 685 388 18 13 673 3 621 6.48e-79 263
MS.gene24603.t1 MTR_2g093740 38.221 416 198 10 29 430 56 426 2.49e-77 261
MS.gene24603.t1 MTR_2g014770 36.446 439 214 11 25 452 51 435 2.57e-77 259
MS.gene24603.t1 MTR_2g093740 38.221 416 198 10 29 430 56 426 8.01e-77 261
MS.gene24603.t1 MTR_2g014770 36.446 439 214 11 25 452 51 435 1.15e-76 259
MS.gene24603.t1 MTR_2g093740 38.221 416 198 10 29 430 56 426 1.69e-76 261
MS.gene24603.t1 MTR_8g100050 36.256 422 217 8 5 419 33 409 6.93e-75 255
MS.gene24603.t1 MTR_1g024025 37.209 387 190 6 22 401 36 376 1.42e-74 261
MS.gene24603.t1 MTR_2g005240 31.587 668 358 19 29 673 23 614 3.29e-74 252
MS.gene24603.t1 MTR_8g100050 36.256 422 217 8 5 419 33 409 4.25e-74 255
MS.gene24603.t1 MTR_2g093740 37.063 429 198 11 29 430 56 439 1.53e-73 251
MS.gene24603.t1 MTR_2g093740 37.063 429 198 11 29 430 56 439 5.67e-73 251
MS.gene24603.t1 MTR_2g093740 37.063 429 198 11 29 430 56 439 1.03e-72 251
MS.gene24603.t1 MTR_4g088210 32.810 573 292 18 25 579 36 533 1.86e-72 248
MS.gene24603.t1 MTR_4g088210 32.810 573 292 18 25 579 36 533 2.13e-72 248
MS.gene24603.t1 MTR_4g060460 36.867 415 204 8 29 427 54 426 3.34e-72 249
MS.gene24603.t1 MTR_4g060460 36.867 415 204 8 29 427 54 426 3.81e-72 249
MS.gene24603.t1 MTR_2g006270 34.414 401 194 11 28 419 12 352 9.58e-72 240
MS.gene24603.t1 MTR_5g040740 37.867 375 165 7 26 391 9 324 1.03e-71 238
MS.gene24603.t1 MTR_5g076270 35.038 391 204 7 23 407 18 364 1.63e-70 245
MS.gene24603.t1 MTR_8g079492 36.000 400 206 8 20 414 14 368 9.76e-70 244
MS.gene24603.t1 MTR_8g079492 36.000 400 206 8 20 414 14 368 9.87e-70 244
MS.gene24603.t1 MTR_4g124900 37.965 403 200 7 17 414 11 368 1.34e-69 246
MS.gene24603.t1 MTR_4g124900 37.965 403 200 7 17 414 11 368 1.64e-69 246
MS.gene24603.t1 MTR_8g101360 38.601 386 185 6 26 404 28 368 9.85e-69 243
MS.gene24603.t1 MTR_2g018690 36.409 401 204 9 20 414 14 369 1.57e-68 241
MS.gene24603.t1 MTR_3g064050 34.271 391 207 8 23 407 20 366 8.59e-66 233
MS.gene24603.t1 MTR_2g006380 33.251 403 193 12 26 419 17 352 6.19e-65 221
MS.gene24603.t1 MTR_8g027440 37.469 403 191 10 29 422 29 379 1.71e-64 226
MS.gene24603.t1 MTR_7g062540 34.753 446 219 14 17 453 4 386 6.84e-64 224
MS.gene24603.t1 MTR_2g093740 40.461 304 134 5 129 430 38 296 4.81e-59 210
MS.gene24603.t1 MTR_2g093740 40.461 304 134 5 129 430 38 296 7.54e-59 210
MS.gene24603.t1 MTR_2g043250 47.059 221 110 2 17 232 11 229 5.32e-58 213
MS.gene24603.t1 MTR_2g043250 40.777 103 60 1 302 404 257 358 8.68e-17 85.5
MS.gene24603.t1 MTR_5g060780 35.749 414 194 15 26 427 9 362 2.99e-57 199
MS.gene24603.t1 MTR_5g061890 34.821 448 209 18 26 450 9 396 5.77e-57 198
MS.gene24603.t1 MTR_5g460920 35.507 414 195 15 26 427 9 362 3.12e-56 196
MS.gene24603.t1 MTR_2g093740 38.801 317 134 6 129 430 38 309 1.40e-55 200
MS.gene24603.t1 MTR_2g093740 38.801 317 134 6 129 430 38 309 2.42e-55 201
MS.gene24603.t1 MTR_8g079492 35.866 329 166 7 86 414 3 286 4.73e-52 193
MS.gene24603.t1 MTR_5g060630 29.828 523 246 18 24 477 7 477 5.22e-50 182
MS.gene24603.t1 MTR_5g460740 29.828 523 246 18 24 477 7 477 5.22e-50 182
MS.gene24603.t1 MTR_5g062970 41.667 192 106 4 29 217 38 226 7.92e-47 166
MS.gene24603.t1 MTR_8g446900 29.630 378 197 11 75 450 12 322 7.74e-44 162
MS.gene24603.t1 MTR_5g062960 35.955 178 88 6 295 469 1 155 6.07e-23 97.4
MS.gene24603.t1 MTR_3g064050 39.216 102 60 2 307 407 29 129 3.50e-15 79.7
MS.gene24603.t1 MTR_5g060770 32.895 152 87 5 74 223 6 144 3.81e-15 77.8
MS.gene24603.t1 MTR_5g060770 32.990 194 100 8 293 476 142 315 1.08e-14 76.6
MS.gene24603.t1 MTR_5g032260 38.095 105 63 2 295 398 49 152 1.70e-14 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24603.t1 AT4G30080 55.255 666 254 13 22 673 12 647 0.0 676
MS.gene24603.t1 AT2G28350 53.148 683 250 18 21 673 2 644 0.0 635
MS.gene24603.t1 AT2G28350 53.148 683 250 18 21 673 2 644 0.0 635
MS.gene24603.t1 AT1G77850 42.358 458 233 11 24 471 14 450 4.63e-114 355
MS.gene24603.t1 AT1G77850 42.358 458 233 11 24 471 14 450 6.34e-114 355
MS.gene24603.t1 AT1G59750 31.571 662 357 16 29 673 22 604 6.33e-78 262
MS.gene24603.t1 AT1G59750 31.361 676 381 14 11 673 4 609 2.15e-77 261
MS.gene24603.t1 AT1G59750 31.361 676 381 14 11 673 4 609 2.15e-77 261
MS.gene24603.t1 AT1G59750 31.213 676 379 15 11 673 4 606 9.12e-77 259
MS.gene24603.t1 AT1G19850 37.859 383 186 6 26 401 40 377 5.06e-76 261
MS.gene24603.t1 AT2G33860 35.792 461 233 11 25 470 52 464 5.51e-76 255
MS.gene24603.t1 AT1G19850 37.859 383 186 6 26 401 51 388 7.49e-76 261
MS.gene24603.t1 AT5G60450 37.838 407 189 11 29 419 41 399 8.73e-75 256
MS.gene24603.t1 AT5G60450 37.838 407 189 11 29 419 65 423 1.34e-74 256
MS.gene24603.t1 AT5G37020 36.041 394 200 6 21 407 14 362 2.23e-73 252
MS.gene24603.t1 AT5G37020 36.041 394 200 6 21 407 14 362 4.83e-73 252
MS.gene24603.t1 AT5G62000 35.407 418 212 8 14 419 40 411 1.89e-71 248
MS.gene24603.t1 AT5G62000 35.407 418 212 8 14 419 40 411 1.89e-71 248
MS.gene24603.t1 AT5G62000 35.407 418 212 8 14 419 40 411 1.89e-71 248
MS.gene24603.t1 AT5G62000 35.407 418 212 8 14 419 40 411 1.89e-71 248
MS.gene24603.t1 AT5G62000 35.407 418 212 8 14 419 40 411 2.80e-71 248
MS.gene24603.t1 AT4G23980 29.925 665 359 18 29 673 12 589 4.17e-71 243
MS.gene24603.t1 AT2G46530 29.293 693 330 21 2 673 3 556 5.06e-71 242
MS.gene24603.t1 AT3G61830 34.899 447 229 12 5 444 1 392 1.20e-70 241
MS.gene24603.t1 AT2G46530 29.730 666 317 20 29 673 42 577 1.48e-70 241
MS.gene24603.t1 AT1G30330 35.910 401 206 9 20 414 14 369 6.85e-69 242
MS.gene24603.t1 AT1G30330 35.930 398 204 9 23 414 19 371 8.61e-69 242
MS.gene24603.t1 AT1G30330 35.930 398 204 9 23 414 19 371 8.61e-69 242
MS.gene24603.t1 AT4G23980 29.624 665 359 19 29 673 12 587 1.30e-68 236
MS.gene24603.t1 AT5G20730 36.473 414 213 6 10 418 5 373 3.73e-67 239
MS.gene24603.t1 AT1G19220 36.855 407 207 6 17 418 11 372 1.26e-66 238
MS.gene24603.t1 AT5G20730 36.386 415 213 7 10 418 5 374 7.06e-66 236
MS.gene24603.t1 AT5G20730 36.386 415 213 7 10 418 5 374 8.78e-66 235
MS.gene24603.t1 AT1G35520 29.365 630 328 19 29 648 27 549 3.49e-60 212
MS.gene24603.t1 AT1G34410 28.463 657 352 19 29 673 27 577 1.67e-59 211
MS.gene24603.t1 AT3G61830 35.925 373 183 10 73 444 24 341 9.41e-58 205
MS.gene24603.t1 AT1G34390 29.253 629 328 21 29 648 27 547 1.59e-57 205
MS.gene24603.t1 AT1G34310 34.988 403 196 11 29 422 27 372 1.88e-57 205
MS.gene24603.t1 AT1G35240 34.048 420 210 11 29 439 27 388 2.47e-57 204
MS.gene24603.t1 AT1G35540 34.336 399 199 10 29 419 27 370 6.55e-57 204
MS.gene24603.t1 AT2G33860 36.467 351 169 8 129 470 2 307 4.69e-56 197
MS.gene24603.t1 AT1G34170 32.587 402 204 11 29 420 25 369 1.61e-53 192
MS.gene24603.t1 AT5G37020 36.429 280 134 4 129 407 31 267 2.16e-48 181
MS.gene24603.t1 AT1G34170 31.095 402 184 11 29 420 25 343 4.32e-47 174
MS.gene24603.t1 AT2G46530 37.455 275 124 6 128 401 36 263 9.24e-47 173
MS.gene24603.t1 AT1G34170 31.095 402 184 11 29 420 25 343 2.16e-46 173
MS.gene24603.t1 AT1G43950 41.346 208 102 7 29 228 27 222 3.71e-38 141
MS.gene24603.t1 AT2G46870 34.862 109 64 2 118 226 24 125 2.86e-11 65.5
MS.gene24603.t1 AT3G25730 36.458 96 59 1 129 222 183 278 6.20e-11 64.7
MS.gene24603.t1 AT3G61970 27.670 206 120 7 121 305 4 201 7.58e-11 64.3

Find 179 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAGATGAATATATTTA+TGG 0.118268 1.3:+46317032 None:intergenic
GTCTCGAATAAGTTGGTTTA+TGG 0.152750 1.3:+46318088 MS.gene24603:CDS
AGATGGAAATACCAATAAAA+TGG 0.189014 1.3:+46319030 MS.gene24603:CDS
GCATAAACCTCATCTGTTTC+AGG 0.203134 1.3:-46317300 None:intergenic
TCCTCCTCTTCAGAACATTT+TGG 0.205669 1.3:+46317527 MS.gene24603:CDS
TGAGAAAGGTGAAAGGTTTA+TGG 0.219953 1.3:-46318570 None:intergenic
CGAAGAAACGGAAGCAAGTT+TGG 0.233645 1.3:+46319147 MS.gene24603:CDS
TGGTTTATGGGAATTGTATC+TGG 0.292586 1.3:+46318101 MS.gene24603:CDS
TCATTATGGTCTTCCGATTT+CGG 0.301421 1.3:+46318751 MS.gene24603:CDS
GAAACGATGGCACTGAAATT+TGG 0.305036 1.3:-46318646 None:intergenic
GGACAATTCATGAAGTTAAC+AGG 0.315804 1.3:-46317222 None:intergenic
CTTGTTCCTTTGAGTGGAAA+TGG 0.317165 1.3:+46317333 MS.gene24603:CDS
TCGTTTCCTAGTAACCTTCT+AGG 0.317283 1.3:+46318662 MS.gene24603:CDS
AGTTCAGGCAGGTCTATTTC+CGG 0.319570 1.3:+46318790 MS.gene24603:CDS
TTCACCTTGGAGGCTTCTTC+AGG 0.328238 1.3:+46318157 MS.gene24603:CDS
AGAAGCCTCCAAGGTGAATT+AGG 0.335922 1.3:-46318152 None:intergenic
ATGCAGATTCGATGGTGTTC+TGG 0.343933 1.3:+46318029 MS.gene24603:CDS
ACAATTGACCTTTCTTTACT+TGG 0.344982 1.3:+46319220 MS.gene24603:CDS
ACCGCTTGAAGGACCGCTTC+TGG 0.350997 1.3:-46317909 None:intergenic
TCTCGAATAAGTTGGTTTAT+GGG 0.357035 1.3:+46318089 MS.gene24603:CDS
CATGCCAGCAGGAGTACTTT+CGG 0.368965 1.3:-46318711 None:intergenic
ACGGCGAGACGTGGAAGTTT+AGG 0.368973 1.3:+46317562 MS.gene24603:CDS
GACAATCTTAATTTCTTCCT+TGG 0.376339 1.3:-46318596 None:intergenic
CATGAGGATCCATTACCATT+AGG 0.376847 1.3:+46317750 MS.gene24603:CDS
CTCTCACTAGAGGAGCGTTT+TGG 0.379568 1.3:-46319100 None:intergenic
CTGGCTCCCTGCATGCCAGC+AGG 0.380532 1.3:-46318722 None:intergenic
CGCGGTTATCCTTATACCAT+TGG 0.383301 1.3:-46319126 None:intergenic
TTCTTCCTTGGTTGTGAGAA+AGG 0.385724 1.3:-46318584 None:intergenic
TGAGAGTTTCCAATGGTATA+AGG 0.391430 1.3:+46319117 MS.gene24603:CDS
CCGCTAAAGTTACCGCTTGA+AGG 0.392870 1.3:-46317920 None:intergenic
CTGCTGGCATGCAGGGAGCC+AGG 0.396598 1.3:+46318723 MS.gene24603:CDS
AACTTGCTTCCGTTTCTTCG+CGG 0.399377 1.3:-46319144 None:intergenic
AGTTTGTTGATCCCCTTCTC+TGG 0.400220 1.3:+46318129 MS.gene24603:CDS
AACTCAGGCGCGAATATGAC+TGG 0.403386 1.3:+46317870 MS.gene24603:CDS
AATCACAAGAAGCTTCTTGC+CGG 0.403468 1.3:+46317645 MS.gene24603:CDS
AATGTCTTGGCAAATGAAAC+AGG 0.423654 1.3:-46317414 None:intergenic
GGAGCCAGGCATGCTCATTA+TGG 0.423939 1.3:+46318737 MS.gene24603:CDS
TTTCGGGTAGACAACGGAAT+AGG 0.433921 1.3:-46318694 None:intergenic
TGGTATTTCCATCTGATGAA+TGG 0.434165 1.3:-46319021 None:intergenic
GGCTAATAAGCAACCGTTTG+AGG 0.435944 1.3:+46317941 MS.gene24603:CDS
CCAACAGGATTCCACCCTAA+TGG 0.436228 1.3:-46317765 None:intergenic
AGAAAGCTGGCAGACATGTT+TGG 0.443426 1.3:+46319262 MS.gene24603:CDS
GTGCTCTACCGTGATATATC+TGG 0.445579 1.3:+46319313 MS.gene24603:CDS
GGAATCAAAGGTGAAACCTT+TGG 0.447087 1.3:-46317243 None:intergenic
TACACAGAATGAGAATCAAA+TGG 0.448127 1.3:+46317812 MS.gene24603:CDS
ATGAGGATCCATTACCATTA+GGG 0.450034 1.3:+46317751 MS.gene24603:CDS
CCTTTGAGTGGAAATGGAAA+TGG 0.450640 1.3:+46317339 MS.gene24603:CDS
TCTGGCCTAATTCACCTTGG+AGG 0.451766 1.3:+46318147 MS.gene24603:CDS
TCTCCACCTCAGTAAAGTTC+AGG 0.453129 1.3:+46318775 MS.gene24603:CDS
ACACAGAATGAGAATCAAAT+GGG 0.454771 1.3:+46317813 MS.gene24603:CDS
TAAGGATAACCGCGAAGAAA+CGG 0.457301 1.3:+46319135 MS.gene24603:CDS
ATGATCAAAAGGCACGAAAC+CGG 0.458029 1.3:-46318813 None:intergenic
GTTTGTGATTCTTACCTGAA+TGG 0.461365 1.3:-46319361 None:intergenic
GATGGAAATACCAATAAAAT+GGG 0.463679 1.3:+46319031 MS.gene24603:CDS
ATGCCAGCAGGAGTACTTTC+GGG 0.465859 1.3:-46318710 None:intergenic
CTTAAGCTTGTTCCTTTGAG+TGG 0.466216 1.3:+46317327 MS.gene24603:CDS
CAGCTATGGCATGCTTGTGC+AGG 0.466746 1.3:+46317123 MS.gene24603:CDS
TGGGATTCGACGAGCGAAGA+AGG 0.469310 1.3:+46317713 MS.gene24603:CDS
TTTATAGATAAAACTTTGCA+TGG 0.470044 1.3:-46317264 None:intergenic
CACTGAAATTTGGCCGTCGA+GGG 0.472008 1.3:-46318636 None:intergenic
GTAATTAGAGATTTGTTGCT+CGG 0.475344 1.3:-46318999 None:intergenic
ATAGGTTGCTCTGACCTAGA+AGG 0.479580 1.3:-46318676 None:intergenic
TGCATTGCCGCCTCTACTAA+AGG 0.482510 1.3:-46318011 None:intergenic
CTGAGGTGGAGATCCGAAAT+CGG 0.482775 1.3:-46318764 None:intergenic
GGATTCCACCCTAATGGTAA+TGG 0.485472 1.3:-46317759 None:intergenic
ATCACAAGAAGCTTCTTGCC+GGG 0.486243 1.3:+46317646 MS.gene24603:CDS
AAGTACTCCTGCTGGCATGC+AGG 0.486614 1.3:+46318715 MS.gene24603:CDS
AAAATGGTGATCTTTGTGTT+GGG 0.488407 1.3:+46317694 MS.gene24603:CDS
CCATTTCCATTTCCACTCAA+AGG 0.488607 1.3:-46317339 None:intergenic
AGACGAATCCTCGGTTTCAA+AGG 0.490672 1.3:-46318069 None:intergenic
CGACGAGCGAAGAAGGGTAT+TGG 0.491306 1.3:+46317720 MS.gene24603:CDS
TTCTCTGGCCTAATTCACCT+TGG 0.492130 1.3:+46318144 MS.gene24603:CDS
TCTAATTACCATTCATCAGA+TGG 0.497333 1.3:+46319013 MS.gene24603:CDS
AAAACTTTGCATGGAATCAA+AGG 0.504526 1.3:-46317255 None:intergenic
ATTTACTGCAAAATGTAAGA+AGG 0.509927 1.3:+46318507 MS.gene24603:CDS
AGTTTAGCAGCATGATCAAA+AGG 0.510146 1.3:-46318824 None:intergenic
CTCTGACCTAGAAGGTTACT+AGG 0.513995 1.3:-46318668 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.517507 1.3:+46317576 MS.gene24603:CDS
TGTCTCTTGCTTACTATCCA+TGG 0.518182 1.3:+46318898 MS.gene24603:CDS
CCATTAGGGTGGAATCCTGT+TGG 0.519653 1.3:+46317765 MS.gene24603:CDS
GGAAGCAAGTTTGGAGAGTA+CGG 0.521728 1.3:+46319156 MS.gene24603:CDS
TGACTGGGAGGGAAAAGGTT+AGG 0.521859 1.3:+46317886 MS.gene24603:CDS
TAAACTTTACTGTTCACTGT+TGG 0.522539 1.3:-46317162 None:intergenic
AAGATGTTCACGGCGAGACG+TGG 0.523046 1.3:+46317553 MS.gene24603:CDS
TGATGAACTGTACAGAAAGC+TGG 0.523736 1.3:+46319249 MS.gene24603:CDS
GGGTAGTAAATAACCTCAAA+CGG 0.525195 1.3:-46317954 None:intergenic
CATAAGATCCAAGTAAAGAA+AGG 0.525657 1.3:-46319228 None:intergenic
ACGAGGAGTTCCCGAACCAT+CGG 0.527122 1.3:-46319062 None:intergenic
GCCAAAATGTTCTGAAGAGG+AGG 0.531494 1.3:-46317528 None:intergenic
TTCTGTGTAAACCAAGGAAG+AGG 0.532226 1.3:-46317798 None:intergenic
GAATATGACTGGGAGGGAAA+AGG 0.532595 1.3:+46317881 MS.gene24603:CDS
ATGTAAGAAGGGTGAGTCCA+TGG 0.533350 1.3:+46318519 MS.gene24603:CDS
AGGATTCGTCTCGAATAAGT+TGG 0.533362 1.3:+46318081 MS.gene24603:CDS
GAAAATGGTGATCTTTGTGT+TGG 0.533797 1.3:+46317693 MS.gene24603:CDS
ACATTGGAGAGGAACCATTC+AGG 0.535436 1.3:+46319347 MS.gene24603:CDS
AGTACTCCTGCTGGCATGCA+GGG 0.540145 1.3:+46318716 MS.gene24603:CDS
AAGACCATAATGAGCATGCC+TGG 0.540953 1.3:-46318741 None:intergenic
GCATGCTTGTGCAGGTGGAA+TGG 0.542227 1.3:+46317131 MS.gene24603:CDS
CCTTCAAGCGGTAACTTTAG+CGG 0.549169 1.3:+46317920 MS.gene24603:CDS
GCGAAGAAGGGTATTGGTGG+TGG 0.550842 1.3:+46317726 MS.gene24603:CDS
GGGATTCGACGAGCGAAGAA+GGG 0.551807 1.3:+46317714 MS.gene24603:CDS
AAAGTTTATTACTTTCCTCA+AGG 0.553230 1.3:+46317177 MS.gene24603:CDS
CTATGGCATGCTTGTGCAGG+TGG 0.553461 1.3:+46317126 MS.gene24603:CDS
CTACCCGAAAGTACTCCTGC+TGG 0.554091 1.3:+46318707 MS.gene24603:CDS
TGTGAAGGCGCCTTTAGTAG+AGG 0.555008 1.3:+46318001 MS.gene24603:CDS
ATCAGATTGAGTCAATGTCT+TGG 0.556065 1.3:-46317427 None:intergenic
AAGGTGAATTAGGCCAGAGA+AGG 0.557096 1.3:-46318142 None:intergenic
TAACTTCATGAATTGTCCAA+AGG 0.561840 1.3:+46317227 MS.gene24603:CDS
AATAACAATGGTTTGAACTC+AGG 0.561882 1.3:+46317855 MS.gene24603:CDS
TTTACTGCAAAATGTAAGAA+GGG 0.567494 1.3:+46318508 MS.gene24603:CDS
TTAGCCAAAATGTTCTGAAG+AGG 0.569408 1.3:-46317531 None:intergenic
GCACTGAAATTTGGCCGTCG+AGG 0.570322 1.3:-46318637 None:intergenic
AACTCTGGTGTACTTGCGCG+AGG 0.570842 1.3:-46317975 None:intergenic
CAAGATGGCCTTTGAAACCG+AGG 0.577088 1.3:+46318061 MS.gene24603:CDS
CGAGCGAAGAAGGGTATTGG+TGG 0.577713 1.3:+46317723 MS.gene24603:CDS
GCTACTAATTCAACCAACCA+TGG 0.579195 1.3:-46318536 None:intergenic
AGGTGAATTAGGCCAGAGAA+GGG 0.583510 1.3:-46318141 None:intergenic
ATTAACAACAACAATAACAA+TGG 0.588569 1.3:+46317843 MS.gene24603:CDS
CACCTCAGTAAAGTTCAGGC+AGG 0.589149 1.3:+46318779 MS.gene24603:CDS
CTGCCTGAACTTTACTGAGG+TGG 0.589800 1.3:-46318778 None:intergenic
AAAGGTGAAAGGTTTATGGA+TGG 0.590026 1.3:-46318566 None:intergenic
CTGAAATTTGGCCGTCGAGG+GGG 0.590791 1.3:-46318634 None:intergenic
TCAAAAGGCACGAAACCGGC+CGG 0.591651 1.3:-46318809 None:intergenic
CAGGCAGGTCTATTTCCGGC+CGG 0.592530 1.3:+46318794 MS.gene24603:CDS
GGCGCCTTCACACAAAACTC+TGG 0.594083 1.3:-46317990 None:intergenic
GTAGTCAAAAGATGCCGCCT+CGG 0.596640 1.3:-46317603 None:intergenic
AGGCGGCAATGCAGATTCGA+TGG 0.597819 1.3:+46318021 MS.gene24603:CDS
TCTGATGCTAACAATGGTGG+AGG 0.600675 1.3:+46317444 MS.gene24603:CDS
GTTACCTGAAGAAGCCTCCA+AGG 0.601497 1.3:-46318161 None:intergenic
GCTGCAGATTATGACACTGT+TGG 0.602356 1.3:+46317369 MS.gene24603:CDS
TTCTGGAATAAGATTCAAGA+TGG 0.603114 1.3:+46318046 MS.gene24603:CDS
ACTCATCAGAAGAAGTTGAA+AGG 0.603125 1.3:+46317088 MS.gene24603:CDS
GGGTATTGGTGGTGGACATG+AGG 0.604425 1.3:+46317734 MS.gene24603:CDS
GGTGTTTAGATCCTCAGCTA+TGG 0.607385 1.3:+46317109 MS.gene24603:CDS
TTCTCATTCTGTGTAAACCA+AGG 0.607875 1.3:-46317804 None:intergenic
GCAGATTATGACACTGTTGG+AGG 0.608851 1.3:+46317372 MS.gene24603:CDS
TGGCCGAAAAGAACAAGCTG+AGG 0.609518 1.3:-46318965 None:intergenic
TTGGTTGTGAGAAAGGTGAA+AGG 0.610407 1.3:-46318577 None:intergenic
CAATCTGATGCTAACAATGG+TGG 0.611120 1.3:+46317441 MS.gene24603:CDS
GCATGTTCAGCATGACCTTG+AGG 0.617891 1.3:-46317192 None:intergenic
TCACAAGAAGCTTCTTGCCG+GGG 0.620934 1.3:+46317647 MS.gene24603:CDS
TCTGGAGCAGTCAAACACAT+TGG 0.623032 1.3:+46319331 MS.gene24603:CDS
ACTTATTCGAGACGAATCCT+CGG 0.623357 1.3:-46318078 None:intergenic
AGACTGAGTAGAACTTGCCA+TGG 0.625027 1.3:-46318915 None:intergenic
GGTAAGACTTGTTGATGACG+AGG 0.626525 1.3:-46319079 None:intergenic
ACTGAAATTTGGCCGTCGAG+GGG 0.629416 1.3:-46318635 None:intergenic
AGGATCCATTACCATTAGGG+TGG 0.632365 1.3:+46317754 MS.gene24603:CDS
TTTCCCTCGTTTGGATTACG+CGG 0.633180 1.3:+46317500 MS.gene24603:CDS
GCCAGAAGCGGTCCTTCAAG+CGG 0.635688 1.3:+46317908 MS.gene24603:CDS
TTTCTCGAATCAGAAGATGT+CGG 0.638567 1.3:+46319193 MS.gene24603:CDS
GGCTGATCCTGAAACAGATG+AGG 0.642209 1.3:+46317293 MS.gene24603:CDS
TCGAATCAGAAGATGTCGGT+CGG 0.645828 1.3:+46319197 MS.gene24603:CDS
ACTCAGGCGCGAATATGACT+GGG 0.647010 1.3:+46317871 MS.gene24603:CDS
AGGCGCGAATATGACTGGGA+GGG 0.648683 1.3:+46317875 MS.gene24603:CDS
TTTCACCTTTCTCACAACCA+AGG 0.649147 1.3:+46318579 MS.gene24603:CDS
ATCGTCTCGGCACAATACCT+AGG 0.650264 1.3:-46317477 None:intergenic
GGAAAAGGTTAGGCCAGAAG+CGG 0.650274 1.3:+46317896 MS.gene24603:CDS
TGACTGCTCCAGATATATCA+CGG 0.657225 1.3:-46319321 None:intergenic
CAAACGAGGGAAAATCGTCT+CGG 0.663409 1.3:-46317490 None:intergenic
CCATTGGAAACTCTCACTAG+AGG 0.665637 1.3:-46319110 None:intergenic
GCACAAGCATGCCATAGCTG+AGG 0.667181 1.3:-46317120 None:intergenic
CCTCTAGTGAGAGTTTCCAA+TGG 0.667381 1.3:+46319110 MS.gene24603:CDS
ACTCAATCTGATGCTAACAA+TGG 0.675509 1.3:+46317438 MS.gene24603:CDS
AAGAAGGGTGAGTCCATGGT+TGG 0.682554 1.3:+46318523 MS.gene24603:CDS
CAGGCGCGAATATGACTGGG+AGG 0.684044 1.3:+46317874 MS.gene24603:CDS
ACTCTGGTGTACTTGCGCGA+GGG 0.685479 1.3:-46317974 None:intergenic
GAGTACTTTCGGGTAGACAA+CGG 0.687017 1.3:-46318700 None:intergenic
ATTAGGTTATTAGAGACACT+AGG 0.687638 1.3:-46318848 None:intergenic
GGTGAATTAGGCCAGAGAAG+GGG 0.693414 1.3:-46318140 None:intergenic
CAGCCGCGTAATCCAAACGA+GGG 0.693625 1.3:-46317503 None:intergenic
AGCAGTCAAACACATTGGAG+AGG 0.695616 1.3:+46319336 MS.gene24603:CDS
AGAAGGTTACTAGGAAACGA+TGG 0.704217 1.3:-46318659 None:intergenic
GAAGGCGCCTTTAGTAGAGG+CGG 0.708805 1.3:+46318004 MS.gene24603:CDS
CACAGAATGAGAATCAAATG+GGG 0.710437 1.3:+46317814 MS.gene24603:CDS
GGTCATGCTGAACATGCATG+TGG 0.714823 1.3:+46317198 MS.gene24603:CDS
TTTATAGAGGAACACCGAGG+CGG 0.715637 1.3:+46317589 MS.gene24603:CDS
GACCTGCCTGAACTTTACTG+AGG 0.725533 1.3:-46318781 None:intergenic
TCAGCCGCGTAATCCAAACG+AGG 0.747954 1.3:-46317504 None:intergenic
ATATTTATAGAGGAACACCG+AGG 0.862150 1.3:+46317586 MS.gene24603:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATCAAATTTAATAAAATA+TGG - chr1.3:46318188-46318207 None:intergenic 10.0%
!!! AGTTTTATCTATAAAGTATA+TGG + chr1.3:46317272-46317291 MS.gene24603:CDS 15.0%
!! AATTGCATAATACTTGAATA+TGG + chr1.3:46318225-46318244 MS.gene24603:intron 20.0%
!! ATTAACAACAACAATAACAA+TGG + chr1.3:46317843-46317862 MS.gene24603:CDS 20.0%
!! TCATTAATTTAGTAATCAAG+TGG - chr1.3:46318323-46318342 None:intergenic 20.0%
!! TTTATAGATAAAACTTTGCA+TGG - chr1.3:46317267-46317286 None:intergenic 20.0%
!! TTTGTATGATTTCATTTGAT+AGG + chr1.3:46318406-46318425 MS.gene24603:intron 20.0%
!!! ATGATATTTTGTAGGTTAAA+TGG + chr1.3:46318474-46318493 MS.gene24603:intron 20.0%
!!! GTAGTATTATGATATTTTGT+AGG + chr1.3:46318466-46318485 MS.gene24603:intron 20.0%
!!! TGATATTTTGTAGGTTAAAT+GGG + chr1.3:46318475-46318494 MS.gene24603:intron 20.0%
! AAAGTTTATTACTTTCCTCA+AGG + chr1.3:46317177-46317196 MS.gene24603:CDS 25.0%
! AACAGAAACACATTCTTAAA+TGG - chr1.3:46318367-46318386 None:intergenic 25.0%
! AAGTTTAGGCATATTTATAG+AGG + chr1.3:46317576-46317595 MS.gene24603:CDS 25.0%
! AGATGGAAATACCAATAAAA+TGG + chr1.3:46319030-46319049 MS.gene24603:CDS 25.0%
! ATTTACTGCAAAATGTAAGA+AGG + chr1.3:46318507-46318526 MS.gene24603:CDS 25.0%
! ATTTCATTTGATAGGATCAT+AGG + chr1.3:46318414-46318433 MS.gene24603:intron 25.0%
! GATGGAAATACCAATAAAAT+GGG + chr1.3:46319031-46319050 MS.gene24603:CDS 25.0%
! TTTACTGCAAAATGTAAGAA+GGG + chr1.3:46318508-46318527 MS.gene24603:CDS 25.0%
!! GGAAAAATTACCCATTTTAT+TGG - chr1.3:46319044-46319063 None:intergenic 25.0%
AAAACTTTGCATGGAATCAA+AGG - chr1.3:46317258-46317277 None:intergenic 30.0%
AAAATGGTGATCTTTGTGTT+GGG + chr1.3:46317694-46317713 MS.gene24603:CDS 30.0%
AATAACAATGGTTTGAACTC+AGG + chr1.3:46317855-46317874 MS.gene24603:CDS 30.0%
ACAATTGACCTTTCTTTACT+TGG + chr1.3:46319220-46319239 MS.gene24603:CDS 30.0%
ACACAGAATGAGAATCAAAT+GGG + chr1.3:46317813-46317832 MS.gene24603:CDS 30.0%
ATTAGGTTATTAGAGACACT+AGG - chr1.3:46318851-46318870 None:intergenic 30.0%
CATAAGATCCAAGTAAAGAA+AGG - chr1.3:46319231-46319250 None:intergenic 30.0%
GAAATCATACAAAACTCAAC+TGG - chr1.3:46318399-46318418 None:intergenic 30.0%
GACAATCTTAATTTCTTCCT+TGG - chr1.3:46318599-46318618 None:intergenic 30.0%
TAAACTTTACTGTTCACTGT+TGG - chr1.3:46317165-46317184 None:intergenic 30.0%
TAACTTCATGAATTGTCCAA+AGG + chr1.3:46317227-46317246 MS.gene24603:CDS 30.0%
TACACAGAATGAGAATCAAA+TGG + chr1.3:46317812-46317831 MS.gene24603:CDS 30.0%
TCTAATTACCATTCATCAGA+TGG + chr1.3:46319013-46319032 MS.gene24603:CDS 30.0%
TGGCATAGAAAAATATGAGA+AGG + chr1.3:46319282-46319301 MS.gene24603:CDS 30.0%
! AAAATGGGTAATTTTTCCGA+TGG + chr1.3:46319046-46319065 MS.gene24603:CDS 30.0%
! CTTACATTTTGCAGTAAATC+TGG - chr1.3:46318506-46318525 None:intergenic 30.0%
! TGCTAGATTTTTGCATCATT+AGG - chr1.3:46318868-46318887 None:intergenic 30.0%
!! GTAATTAGAGATTTGTTGCT+CGG - chr1.3:46319002-46319021 None:intergenic 30.0%
!! TCTCGAATAAGTTGGTTTAT+GGG + chr1.3:46318089-46318108 MS.gene24603:CDS 30.0%
!! TTCTGGAATAAGATTCAAGA+TGG + chr1.3:46318046-46318065 MS.gene24603:CDS 30.0%
!!! ATTTTGGCTAAAGATGTTCA+CGG + chr1.3:46317543-46317562 MS.gene24603:CDS 30.0%
AAAGGTGAAAGGTTTATGGA+TGG - chr1.3:46318569-46318588 None:intergenic 35.0%
AATGTCTTGGCAAATGAAAC+AGG - chr1.3:46317417-46317436 None:intergenic 35.0%
ACTCAATCTGATGCTAACAA+TGG + chr1.3:46317438-46317457 MS.gene24603:CDS 35.0%
ACTCATCAGAAGAAGTTGAA+AGG + chr1.3:46317088-46317107 MS.gene24603:CDS 35.0%
AGTTTAGCAGCATGATCAAA+AGG - chr1.3:46318827-46318846 None:intergenic 35.0%
ATATTTATAGAGGAACACCG+AGG + chr1.3:46317586-46317605 MS.gene24603:CDS 35.0%
ATCAGATTGAGTCAATGTCT+TGG - chr1.3:46317430-46317449 None:intergenic 35.0%
ATGAGGATCCATTACCATTA+GGG + chr1.3:46317751-46317770 MS.gene24603:CDS 35.0%
CACAGAATGAGAATCAAATG+GGG + chr1.3:46317814-46317833 MS.gene24603:CDS 35.0%
GAAAATGGTGATCTTTGTGT+TGG + chr1.3:46317693-46317712 MS.gene24603:CDS 35.0%
GGACAATTCATGAAGTTAAC+AGG - chr1.3:46317225-46317244 None:intergenic 35.0%
GGGTAGTAAATAACCTCAAA+CGG - chr1.3:46317957-46317976 None:intergenic 35.0%
TCATTATGGTCTTCCGATTT+CGG + chr1.3:46318751-46318770 MS.gene24603:CDS 35.0%
TGAGAAAGGTGAAAGGTTTA+TGG - chr1.3:46318573-46318592 None:intergenic 35.0%
TGAGAGTTTCCAATGGTATA+AGG + chr1.3:46319117-46319136 MS.gene24603:CDS 35.0%
TGGTATTTCCATCTGATGAA+TGG - chr1.3:46319024-46319043 None:intergenic 35.0%
TGGTTTATGGGAATTGTATC+TGG + chr1.3:46318101-46318120 MS.gene24603:CDS 35.0%
TTCTCATTCTGTGTAAACCA+AGG - chr1.3:46317807-46317826 None:intergenic 35.0%
TTTCTCGAATCAGAAGATGT+CGG + chr1.3:46319193-46319212 MS.gene24603:CDS 35.0%
! AGAGGAAAATTTTCTCCAAC+AGG - chr1.3:46317783-46317802 None:intergenic 35.0%
! GAGAAAATTTTCCTCTTCCT+TGG + chr1.3:46317787-46317806 MS.gene24603:CDS 35.0%
!! AAACCTCAGCTTGTTCTTTT+CGG + chr1.3:46318962-46318981 MS.gene24603:CDS 35.0%
!! GTCTCGAATAAGTTGGTTTA+TGG + chr1.3:46318088-46318107 MS.gene24603:CDS 35.0%
!! TTAGCCAAAATGTTCTGAAG+AGG - chr1.3:46317534-46317553 None:intergenic 35.0%
!! TTCGATTGTGTTTTTGAGAG+CGG + chr1.3:46317671-46317690 MS.gene24603:CDS 35.0%
AATCACAAGAAGCTTCTTGC+CGG + chr1.3:46317645-46317664 MS.gene24603:CDS 40.0%
ACTTATTCGAGACGAATCCT+CGG - chr1.3:46318081-46318100 None:intergenic 40.0%
AGAAGGTTACTAGGAAACGA+TGG - chr1.3:46318662-46318681 None:intergenic 40.0%
AGGATTCGTCTCGAATAAGT+TGG + chr1.3:46318081-46318100 MS.gene24603:CDS 40.0%
CAAAAACACAATCGAATCCC+CGG - chr1.3:46317667-46317686 None:intergenic 40.0%
CAATCTGATGCTAACAATGG+TGG + chr1.3:46317441-46317460 MS.gene24603:CDS 40.0%
CATGAGGATCCATTACCATT+AGG + chr1.3:46317750-46317769 MS.gene24603:CDS 40.0%
CCATTTCCATTTCCACTCAA+AGG - chr1.3:46317342-46317361 None:intergenic 40.0%
CCTTTGAGTGGAAATGGAAA+TGG + chr1.3:46317339-46317358 MS.gene24603:CDS 40.0%
CTTGTTCCTTTGAGTGGAAA+TGG + chr1.3:46317333-46317352 MS.gene24603:CDS 40.0%
GCATAAACCTCATCTGTTTC+AGG - chr1.3:46317303-46317322 None:intergenic 40.0%
GCTACTAATTCAACCAACCA+TGG - chr1.3:46318539-46318558 None:intergenic 40.0%
GGAATCAAAGGTGAAACCTT+TGG - chr1.3:46317246-46317265 None:intergenic 40.0%
TAAGGATAACCGCGAAGAAA+CGG + chr1.3:46319135-46319154 MS.gene24603:CDS 40.0%
TCGTTTCCTAGTAACCTTCT+AGG + chr1.3:46318662-46318681 MS.gene24603:CDS 40.0%
TGACTGCTCCAGATATATCA+CGG - chr1.3:46319324-46319343 None:intergenic 40.0%
TGATGAACTGTACAGAAAGC+TGG + chr1.3:46319249-46319268 MS.gene24603:CDS 40.0%
TGTCTCTTGCTTACTATCCA+TGG + chr1.3:46318898-46318917 MS.gene24603:CDS 40.0%
TTCTGTGTAAACCAAGGAAG+AGG - chr1.3:46317801-46317820 None:intergenic 40.0%
TTCTTCCTTGGTTGTGAGAA+AGG - chr1.3:46318587-46318606 None:intergenic 40.0%
TTGGTTGTGAGAAAGGTGAA+AGG - chr1.3:46318580-46318599 None:intergenic 40.0%
TTTCACCTTTCTCACAACCA+AGG + chr1.3:46318579-46318598 MS.gene24603:CDS 40.0%
! ATGATCAAAAGGCACGAAAC+CGG - chr1.3:46318816-46318835 None:intergenic 40.0%
! CTTAAGCTTGTTCCTTTGAG+TGG + chr1.3:46317327-46317346 MS.gene24603:CDS 40.0%
! GAAACGATGGCACTGAAATT+TGG - chr1.3:46318649-46318668 None:intergenic 40.0%
! GGGTAATTTTTCCGATGGTT+CGG + chr1.3:46319051-46319070 MS.gene24603:CDS 40.0%
! GGTAATTTTTCCGATGGTTC+GGG + chr1.3:46319052-46319071 MS.gene24603:CDS 40.0%
! TCCTCCTCTTCAGAACATTT+TGG + chr1.3:46317527-46317546 MS.gene24603:CDS 40.0%
!! GTGTTTTTGAGAGCGGAAAA+TGG + chr1.3:46317678-46317697 MS.gene24603:CDS 40.0%
!! TACACCAGAGTTTTGTGTGA+AGG + chr1.3:46317986-46318005 MS.gene24603:CDS 40.0%
!!! GTTGCTCGGTGAGAATTTTT+TGG - chr1.3:46318988-46319007 None:intergenic 40.0%
AAGACCATAATGAGCATGCC+TGG - chr1.3:46318744-46318763 None:intergenic 45.0%
AAGGTGAATTAGGCCAGAGA+AGG - chr1.3:46318145-46318164 None:intergenic 45.0%
ACATTGGAGAGGAACCATTC+AGG + chr1.3:46319347-46319366 MS.gene24603:CDS 45.0%
AGAAAGCTGGCAGACATGTT+TGG + chr1.3:46319262-46319281 MS.gene24603:CDS 45.0%
AGAAGCCTCCAAGGTGAATT+AGG - chr1.3:46318155-46318174 None:intergenic 45.0%
AGACGAATCCTCGGTTTCAA+AGG - chr1.3:46318072-46318091 None:intergenic 45.0%
AGACTGAGTAGAACTTGCCA+TGG - chr1.3:46318918-46318937 None:intergenic 45.0%
AGCAGTCAAACACATTGGAG+AGG + chr1.3:46319336-46319355 MS.gene24603:CDS 45.0%
AGGATCCATTACCATTAGGG+TGG + chr1.3:46317754-46317773 MS.gene24603:CDS 45.0%
AGGTGAATTAGGCCAGAGAA+GGG - chr1.3:46318144-46318163 None:intergenic 45.0%
AGTTCAGGCAGGTCTATTTC+CGG + chr1.3:46318790-46318809 MS.gene24603:CDS 45.0%
ATAGGTTGCTCTGACCTAGA+AGG - chr1.3:46318679-46318698 None:intergenic 45.0%
ATCACAAGAAGCTTCTTGCC+GGG + chr1.3:46317646-46317665 MS.gene24603:CDS 45.0%
ATGCAGATTCGATGGTGTTC+TGG + chr1.3:46318029-46318048 MS.gene24603:CDS 45.0%
ATGTAAGAAGGGTGAGTCCA+TGG + chr1.3:46318519-46318538 MS.gene24603:CDS 45.0%
CAAACGAGGGAAAATCGTCT+CGG - chr1.3:46317493-46317512 None:intergenic 45.0%
CCATTGGAAACTCTCACTAG+AGG - chr1.3:46319113-46319132 None:intergenic 45.0%
CCTCTAGTGAGAGTTTCCAA+TGG + chr1.3:46319110-46319129 MS.gene24603:CDS 45.0%
CCTTCAAGCGGTAACTTTAG+CGG + chr1.3:46317920-46317939 MS.gene24603:CDS 45.0%
CGAAGAAACGGAAGCAAGTT+TGG + chr1.3:46319147-46319166 MS.gene24603:CDS 45.0%
CGCGGTTATCCTTATACCAT+TGG - chr1.3:46319129-46319148 None:intergenic 45.0%
CTCTGACCTAGAAGGTTACT+AGG - chr1.3:46318671-46318690 None:intergenic 45.0%
GAATATGACTGGGAGGGAAA+AGG + chr1.3:46317881-46317900 MS.gene24603:CDS 45.0%
GCAGATTATGACACTGTTGG+AGG + chr1.3:46317372-46317391 MS.gene24603:CDS 45.0%
GCTGCAGATTATGACACTGT+TGG + chr1.3:46317369-46317388 MS.gene24603:CDS 45.0%
GGAAGCAAGTTTGGAGAGTA+CGG + chr1.3:46319156-46319175 MS.gene24603:CDS 45.0%
GGATTCCACCCTAATGGTAA+TGG - chr1.3:46317762-46317781 None:intergenic 45.0%
GGCTAATAAGCAACCGTTTG+AGG + chr1.3:46317941-46317960 MS.gene24603:CDS 45.0%
GGTGTTTAGATCCTCAGCTA+TGG + chr1.3:46317109-46317128 MS.gene24603:CDS 45.0%
GTGCTCTACCGTGATATATC+TGG + chr1.3:46319313-46319332 MS.gene24603:CDS 45.0%
TCGAATCAGAAGATGTCGGT+CGG + chr1.3:46319197-46319216 MS.gene24603:CDS 45.0%
TCTCCACCTCAGTAAAGTTC+AGG + chr1.3:46318775-46318794 MS.gene24603:CDS 45.0%
TCTGATGCTAACAATGGTGG+AGG + chr1.3:46317444-46317463 MS.gene24603:CDS 45.0%
TTCTCTGGCCTAATTCACCT+TGG + chr1.3:46318144-46318163 MS.gene24603:CDS 45.0%
TTTATAGAGGAACACCGAGG+CGG + chr1.3:46317589-46317608 MS.gene24603:CDS 45.0%
TTTCCCTCGTTTGGATTACG+CGG + chr1.3:46317500-46317519 MS.gene24603:CDS 45.0%
! AACTTGCTTCCGTTTCTTCG+CGG - chr1.3:46319147-46319166 None:intergenic 45.0%
! GAGTACTTTCGGGTAGACAA+CGG - chr1.3:46318703-46318722 None:intergenic 45.0%
! GTGGAGGTTTTTCTGTTCCT+AGG + chr1.3:46317460-46317479 MS.gene24603:CDS 45.0%
! TCTGGAGCAGTCAAACACAT+TGG + chr1.3:46319331-46319350 MS.gene24603:CDS 45.0%
! TTTCGGGTAGACAACGGAAT+AGG - chr1.3:46318697-46318716 None:intergenic 45.0%
!! AGTTTGTTGATCCCCTTCTC+TGG + chr1.3:46318129-46318148 MS.gene24603:CDS 45.0%
!! GCCAAAATGTTCTGAAGAGG+AGG - chr1.3:46317531-46317550 None:intergenic 45.0%
!! GGCATCTTTTGACTACTGGT+TGG + chr1.3:46317610-46317629 MS.gene24603:CDS 45.0%
!! GGTAAGACTTGTTGATGACG+AGG - chr1.3:46319082-46319101 None:intergenic 45.0%
AACTCAGGCGCGAATATGAC+TGG + chr1.3:46317870-46317889 MS.gene24603:CDS 50.0%
AAGAAGGGTGAGTCCATGGT+TGG + chr1.3:46318523-46318542 MS.gene24603:CDS 50.0%
ACGCGTAATGCATAGTGCGA+AGG - chr1.3:46318287-46318306 None:intergenic 50.0%
ACTCAGGCGCGAATATGACT+GGG + chr1.3:46317871-46317890 MS.gene24603:CDS 50.0%
ACTGAAATTTGGCCGTCGAG+GGG - chr1.3:46318638-46318657 None:intergenic 50.0%
ATCGTCTCGGCACAATACCT+AGG - chr1.3:46317480-46317499 None:intergenic 50.0%
CACCTCAGTAAAGTTCAGGC+AGG + chr1.3:46318779-46318798 MS.gene24603:CDS 50.0%
CACTGAAATTTGGCCGTCGA+GGG - chr1.3:46318639-46318658 None:intergenic 50.0%
CCAACAGGATTCCACCCTAA+TGG - chr1.3:46317768-46317787 None:intergenic 50.0%
CCATTAGGGTGGAATCCTGT+TGG + chr1.3:46317765-46317784 MS.gene24603:CDS 50.0%
CCGCTAAAGTTACCGCTTGA+AGG - chr1.3:46317923-46317942 None:intergenic 50.0%
CTGAGGTGGAGATCCGAAAT+CGG - chr1.3:46318767-46318786 None:intergenic 50.0%
CTGCCTGAACTTTACTGAGG+TGG - chr1.3:46318781-46318800 None:intergenic 50.0%
GACCTGCCTGAACTTTACTG+AGG - chr1.3:46318784-46318803 None:intergenic 50.0%
GCATGTTCAGCATGACCTTG+AGG - chr1.3:46317195-46317214 None:intergenic 50.0%
GGAAAAGGTTAGGCCAGAAG+CGG + chr1.3:46317896-46317915 MS.gene24603:CDS 50.0%
GGCTGATCCTGAAACAGATG+AGG + chr1.3:46317293-46317312 MS.gene24603:CDS 50.0%
GGTCATGCTGAACATGCATG+TGG + chr1.3:46317198-46317217 MS.gene24603:CDS 50.0%
GGTGAATTAGGCCAGAGAAG+GGG - chr1.3:46318143-46318162 None:intergenic 50.0%
GTAGTCAAAAGATGCCGCCT+CGG - chr1.3:46317606-46317625 None:intergenic 50.0%
GTTACCTGAAGAAGCCTCCA+AGG - chr1.3:46318164-46318183 None:intergenic 50.0%
TCACAAGAAGCTTCTTGCCG+GGG + chr1.3:46317647-46317666 MS.gene24603:CDS 50.0%
TCTGGCCTAATTCACCTTGG+AGG + chr1.3:46318147-46318166 MS.gene24603:CDS 50.0%
TGACTGGGAGGGAAAAGGTT+AGG + chr1.3:46317886-46317905 MS.gene24603:CDS 50.0%
TGCATTGCCGCCTCTACTAA+AGG - chr1.3:46318014-46318033 None:intergenic 50.0%
TGGCCGAAAAGAACAAGCTG+AGG - chr1.3:46318968-46318987 None:intergenic 50.0%
TTCACCTTGGAGGCTTCTTC+AGG + chr1.3:46318157-46318176 MS.gene24603:CDS 50.0%
! CGAGACGATTTTCCCTCGTT+TGG + chr1.3:46317491-46317510 MS.gene24603:CDS 50.0%
!! AGGCGGCATCTTTTGACTAC+TGG + chr1.3:46317606-46317625 MS.gene24603:CDS 50.0%
!! ATGCCAGCAGGAGTACTTTC+GGG - chr1.3:46318713-46318732 None:intergenic 50.0%
!! CAAGATGGCCTTTGAAACCG+AGG + chr1.3:46318061-46318080 MS.gene24603:CDS 50.0%
!! CATGCCAGCAGGAGTACTTT+CGG - chr1.3:46318714-46318733 None:intergenic 50.0%
!! TGTGAAGGCGCCTTTAGTAG+AGG + chr1.3:46318001-46318020 MS.gene24603:CDS 50.0%
!!! CTCTCACTAGAGGAGCGTTT+TGG - chr1.3:46319103-46319122 None:intergenic 50.0%
AACTCTGGTGTACTTGCGCG+AGG - chr1.3:46317978-46317997 None:intergenic 55.0%
AAGATGTTCACGGCGAGACG+TGG + chr1.3:46317553-46317572 MS.gene24603:CDS 55.0%
AAGTACTCCTGCTGGCATGC+AGG + chr1.3:46318715-46318734 MS.gene24603:CDS 55.0%
ACGAGGAGTTCCCGAACCAT+CGG - chr1.3:46319065-46319084 None:intergenic 55.0%
ACGGCGAGACGTGGAAGTTT+AGG + chr1.3:46317562-46317581 MS.gene24603:CDS 55.0%
ACTCTGGTGTACTTGCGCGA+GGG - chr1.3:46317977-46317996 None:intergenic 55.0%
AGGCGCGAATATGACTGGGA+GGG + chr1.3:46317875-46317894 MS.gene24603:CDS 55.0%
AGGCGGCAATGCAGATTCGA+TGG + chr1.3:46318021-46318040 MS.gene24603:CDS 55.0%
AGTACTCCTGCTGGCATGCA+GGG + chr1.3:46318716-46318735 MS.gene24603:CDS 55.0%
CAGCCGCGTAATCCAAACGA+GGG - chr1.3:46317506-46317525 None:intergenic 55.0%
CAGCTATGGCATGCTTGTGC+AGG + chr1.3:46317123-46317142 MS.gene24603:CDS 55.0%
CTATGGCATGCTTGTGCAGG+TGG + chr1.3:46317126-46317145 MS.gene24603:CDS 55.0%
CTGAAATTTGGCCGTCGAGG+GGG - chr1.3:46318637-46318656 None:intergenic 55.0%
GCACAAGCATGCCATAGCTG+AGG - chr1.3:46317123-46317142 None:intergenic 55.0%
GCACTGAAATTTGGCCGTCG+AGG - chr1.3:46318640-46318659 None:intergenic 55.0%
GCATGCTTGTGCAGGTGGAA+TGG + chr1.3:46317131-46317150 MS.gene24603:CDS 55.0%
GGAGCCAGGCATGCTCATTA+TGG + chr1.3:46318737-46318756 MS.gene24603:CDS 55.0%
GGCGCCTTCACACAAAACTC+TGG - chr1.3:46317993-46318012 None:intergenic 55.0%
GGGATTCGACGAGCGAAGAA+GGG + chr1.3:46317714-46317733 MS.gene24603:CDS 55.0%
TCAGCCGCGTAATCCAAACG+AGG - chr1.3:46317507-46317526 None:intergenic 55.0%
TGGGATTCGACGAGCGAAGA+AGG + chr1.3:46317713-46317732 MS.gene24603:CDS 55.0%
! CGACGAGCGAAGAAGGGTAT+TGG + chr1.3:46317720-46317739 MS.gene24603:CDS 55.0%
! TCAAAAGGCACGAAACCGGC+CGG - chr1.3:46318812-46318831 None:intergenic 55.0%
!! CGAGCGAAGAAGGGTATTGG+TGG + chr1.3:46317723-46317742 MS.gene24603:CDS 55.0%
!! CTACCCGAAAGTACTCCTGC+TGG + chr1.3:46318707-46318726 MS.gene24603:CDS 55.0%
!! GAAGGCGCCTTTAGTAGAGG+CGG + chr1.3:46318004-46318023 MS.gene24603:CDS 55.0%
!! GCGAAGAAGGGTATTGGTGG+TGG + chr1.3:46317726-46317745 MS.gene24603:CDS 55.0%
!! GGGTATTGGTGGTGGACATG+AGG + chr1.3:46317734-46317753 MS.gene24603:CDS 55.0%
ACCGCTTGAAGGACCGCTTC+TGG - chr1.3:46317912-46317931 None:intergenic 60.0%
CACCACGATTTCCCCCTCGA+CGG + chr1.3:46318623-46318642 MS.gene24603:CDS 60.0%
CAGGCAGGTCTATTTCCGGC+CGG + chr1.3:46318794-46318813 MS.gene24603:CDS 60.0%
CAGGCGCGAATATGACTGGG+AGG + chr1.3:46317874-46317893 MS.gene24603:CDS 60.0%
GCCAGAAGCGGTCCTTCAAG+CGG + chr1.3:46317908-46317927 MS.gene24603:CDS 60.0%
TCGAGGGGGAAATCGTGGTG+TGG - chr1.3:46318623-46318642 None:intergenic 60.0%
CTGCTGGCATGCAGGGAGCC+AGG + chr1.3:46318723-46318742 MS.gene24603:CDS 70.0%
CTGGCTCCCTGCATGCCAGC+AGG - chr1.3:46318725-46318744 None:intergenic 70.0%
GGCCGTCGAGGGGGAAATCG+TGG - chr1.3:46318628-46318647 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 46317039 46319372 46317039 ID=MS.gene24603
chr1.3 mRNA 46317039 46319372 46317039 ID=MS.gene24603.t1;Parent=MS.gene24603
chr1.3 exon 46317039 46318178 46317039 ID=MS.gene24603.t1.exon1;Parent=MS.gene24603.t1
chr1.3 CDS 46317039 46318178 46317039 ID=cds.MS.gene24603.t1;Parent=MS.gene24603.t1
chr1.3 exon 46318488 46319372 46318488 ID=MS.gene24603.t1.exon2;Parent=MS.gene24603.t1
chr1.3 CDS 46318488 46319372 46318488 ID=cds.MS.gene24603.t1;Parent=MS.gene24603.t1
Gene Sequence

>MS.gene24603

ATGAATATATTTATGGATTCAAGAGATAATTCAAATAATAATAATAACAACTCATCAGAAGAAGTTGAAAGGTGTTTAGATCCTCAGCTATGGCATGCTTGTGCAGGTGGAATGGTTCAGATGCCAACAGTGAACAGTAAAGTTTATTACTTTCCTCAAGGTCATGCTGAACATGCATGTGGACCTGTTAACTTCATGAATTGTCCAAAGGTTTCACCTTTGATTCCATGCAAAGTTTTATCTATAAAGTATATGGCTGATCCTGAAACAGATGAGGTTTATGCTAAGCTTAAGCTTGTTCCTTTGAGTGGAAATGGAAATGGAAATGATGCTGCAGATTATGACACTGTTGGAGGAAATGAAAATCAAGATAAGCCTGTTTCATTTGCCAAGACATTGACTCAATCTGATGCTAACAATGGTGGAGGTTTTTCTGTTCCTAGGTATTGTGCCGAGACGATTTTCCCTCGTTTGGATTACGCGGCTGATCCTCCTCTTCAGAACATTTTGGCTAAAGATGTTCACGGCGAGACGTGGAAGTTTAGGCATATTTATAGAGGAACACCGAGGCGGCATCTTTTGACTACTGGTTGGAGTACTTTTGTTAATCACAAGAAGCTTCTTGCCGGGGATTCGATTGTGTTTTTGAGAGCGGAAAATGGTGATCTTTGTGTTGGGATTCGACGAGCGAAGAAGGGTATTGGTGGTGGACATGAGGATCCATTACCATTAGGGTGGAATCCTGTTGGAGAAAATTTTCCTCTTCCTTGGTTTACACAGAATGAGAATCAAATGGGGAGAAACATTAACAACAACAATAACAATGGTTTGAACTCAGGCGCGAATATGACTGGGAGGGAAAAGGTTAGGCCAGAAGCGGTCCTTCAAGCGGTAACTTTAGCGGCTAATAAGCAACCGTTTGAGGTTATTTACTACCCTCGCGCAAGTACACCAGAGTTTTGTGTGAAGGCGCCTTTAGTAGAGGCGGCAATGCAGATTCGATGGTGTTCTGGAATAAGATTCAAGATGGCCTTTGAAACCGAGGATTCGTCTCGAATAAGTTGGTTTATGGGAATTGTATCTGGAGTTCAGTTTGTTGATCCCCTTCTCTGGCCTAATTCACCTTGGAGGCTTCTTCAGGTAACACCATATTTTATTAAATTTGATGTCAATTAACAGAACAGAAAATTGCATAATACTTGAATATGGTAATCTTCACTCTTTCTAATCTTTAATTTATGTTATCCTTCGCACTATGCATTACGCGTACACGTTGAGTTACCACTTGATTACTAAATTAATGAACTCTGATGTTGTTTGTTAATCCATTTAAGAATGTGTTTCTGTTTTTTTGTTACCAGTTGAGTTTTGTATGATTTCATTTGATAGGATCATAGGAATATTTGCATTGTGTCTACTCATAATAAGTAGTATTATGATATTTTGTAGGTTAAATGGGATGAACCAGATTTACTGCAAAATGTAAGAAGGGTGAGTCCATGGTTGGTTGAATTAGTAGCAAACATTCCATCCATAAACCTTTCACCTTTCTCACAACCAAGGAAGAAATTAAGATTGTCGCCACACCACGATTTCCCCCTCGACGGCCAAATTTCAGTGCCATCGTTTCCTAGTAACCTTCTAGGTCAGAGCAACCTATTCCGTTGTCTACCCGAAAGTACTCCTGCTGGCATGCAGGGAGCCAGGCATGCTCATTATGGTCTTCCGATTTCGGATCTCCACCTCAGTAAAGTTCAGGCAGGTCTATTTCCGGCCGGTTTCGTGCCTTTTGATCATGCTGCTAAACTACCTAGTGTCTCTAATAACCTAATGATGCAAAAATCTAGCACGAGTGAGAATGTCTCTTGCTTACTATCCATGGCAAGTTCTACTCAGTCTTCAAAGAAATTCAACGATGAGAAGAAACCTCAGCTTGTTCTTTTCGGCCAAAAAATTCTCACCGAGCAACAAATCTCTAATTACCATTCATCAGATGGAAATACCAATAAAATGGGTAATTTTTCCGATGGTTCGGGAACTCCTCGTCATCAACAAGTCTTACCAAAACGCTCCTCTAGTGAGAGTTTCCAATGGTATAAGGATAACCGCGAAGAAACGGAAGCAAGTTTGGAGAGTACGGATCACTGTAAAGTTTTTCTCGAATCAGAAGATGTCGGTCGGACAATTGACCTTTCTTTACTTGGATCTTATGATGAACTGTACAGAAAGCTGGCAGACATGTTTGGCATAGAAAAATATGAGAAGGTAAGTCGCGTGCTCTACCGTGATATATCTGGAGCAGTCAAACACATTGGAGAGGAACCATTCAGGTAA
Protein sequence

>MS.gene24603.t1

MNIFMDSRDNSNNNNNNSSEEVERCLDPQLWHACAGGMVQMPTVNSKVYYFPQGHAEHACGPVNFMNCPKVSPLIPCKVLSIKYMADPETDEVYAKLKLVPLSGNGNGNDAADYDTVGGNENQDKPVSFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYAADPPLQNILAKDVHGETWKFRHIYRGTPRRHLLTTGWSTFVNHKKLLAGDSIVFLRAENGDLCVGIRRAKKGIGGGHEDPLPLGWNPVGENFPLPWFTQNENQMGRNINNNNNNGLNSGANMTGREKVRPEAVLQAVTLAANKQPFEVIYYPRASTPEFCVKAPLVEAAMQIRWCSGIRFKMAFETEDSSRISWFMGIVSGVQFVDPLLWPNSPWRLLQVKWDEPDLLQNVRRVSPWLVELVANIPSINLSPFSQPRKKLRLSPHHDFPLDGQISVPSFPSNLLGQSNLFRCLPESTPAGMQGARHAHYGLPISDLHLSKVQAGLFPAGFVPFDHAAKLPSVSNNLMMQKSSTSENVSCLLSMASSTQSSKKFNDEKKPQLVLFGQKILTEQQISNYHSSDGNTNKMGNFSDGSGTPRHQQVLPKRSSSESFQWYKDNREETEASLESTDHCKVFLESEDVGRTIDLSLLGSYDELYRKLADMFGIEKYEKVSRVLYRDISGAVKHIGEEPFR