Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24813.t1 | XP_024639353.1 | 85.5 | 131 | 19 | 0 | 98 | 228 | 1 | 131 | 2.00E-61 | 245.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24813.t1 | G7JX45 | 88.2 | 85 | 10 | 0 | 144 | 228 | 25 | 109 | 2.9e-38 | 168.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene24813.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24813.t1 | MTR_5g053510 | 88.235 | 85 | 10 | 0 | 144 | 228 | 25 | 109 | 1.18e-50 | 160 |
| MS.gene24813.t1 | MTR_4g123470 | 34.314 | 204 | 124 | 5 | 11 | 211 | 14 | 210 | 2.67e-30 | 112 |
| MS.gene24813.t1 | MTR_1g054495 | 27.876 | 226 | 156 | 6 | 1 | 225 | 1 | 220 | 7.03e-25 | 98.2 |
| MS.gene24813.t1 | MTR_0014s0220 | 30.631 | 222 | 131 | 7 | 5 | 225 | 623 | 822 | 3.16e-23 | 97.8 |
| MS.gene24813.t1 | MTR_4g053550 | 52.113 | 71 | 33 | 1 | 24 | 93 | 47 | 117 | 1.51e-19 | 81.3 |
| MS.gene24813.t1 | MTR_4g085790 | 28.000 | 225 | 155 | 5 | 1 | 224 | 1 | 219 | 1.53e-19 | 84.0 |
| MS.gene24813.t1 | MTR_7g025150 | 28.000 | 225 | 155 | 5 | 1 | 224 | 1 | 219 | 4.87e-19 | 82.8 |
| MS.gene24813.t1 | MTR_1g041870 | 32.778 | 180 | 104 | 5 | 49 | 228 | 13 | 175 | 1.36e-16 | 75.1 |
| MS.gene24813.t1 | MTR_1g054485 | 31.343 | 134 | 89 | 3 | 1 | 133 | 1 | 132 | 1.15e-14 | 70.1 |
| MS.gene24813.t1 | MTR_7g039210 | 21.212 | 231 | 152 | 5 | 1 | 203 | 1 | 229 | 7.04e-12 | 63.5 |
| MS.gene24813.t1 | MTR_4g069750 | 29.897 | 97 | 67 | 1 | 1 | 96 | 1 | 97 | 8.82e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGAATTCTTCAAGAAATTT+TGG | 0.074446 | 8.1:-6965280 | None:intergenic |
| CAGACTTCAATGATTTCTTA+TGG | 0.226332 | 8.1:+6965727 | MS.gene24813:CDS |
| AATGTAACTTTCAACAATTC+AGG | 0.262321 | 8.1:+6965352 | MS.gene24813:CDS |
| GATTTGCATTGCAGAGAAAA+AGG | 0.315663 | 8.1:-6965767 | None:intergenic |
| TGTACTCAAACGACAAGTTT+TGG | 0.321936 | 8.1:+6965080 | MS.gene24813:CDS |
| TGGAATCTGATAGATGTTAA+TGG | 0.336468 | 8.1:+6965319 | MS.gene24813:CDS |
| TCGATGATTTGAAACCAGTT+TGG | 0.373591 | 8.1:+6965299 | MS.gene24813:CDS |
| AACGAATAGTGTCTCCAATC+TGG | 0.387174 | 8.1:-6965893 | None:intergenic |
| AGACTTCAATGATTTCTTAT+GGG | 0.387413 | 8.1:+6965728 | MS.gene24813:CDS |
| AATATATACAAAACCTGATC+TGG | 0.390568 | 8.1:-6965126 | None:intergenic |
| AAATAAAATGATTTGCGGTT+CGG | 0.410181 | 8.1:-6965550 | None:intergenic |
| AACTGTACATCTTCCAGATC+AGG | 0.417936 | 8.1:+6965113 | MS.gene24813:CDS |
| TATTCAGCGATTCATTGCTT+TGG | 0.421950 | 8.1:-6965824 | None:intergenic |
| GATGGCAGAAAGATCTGAAT+TGG | 0.422050 | 8.1:+6965597 | MS.gene24813:CDS |
| GCTGTAACAAACTCTTCATT+CGG | 0.423810 | 8.1:-6965789 | None:intergenic |
| CTCTTTCCACTTCAAATGGC+TGG | 0.424705 | 8.1:+6965048 | None:intergenic |
| AGCCGATTCATACTAACTCT+TGG | 0.438013 | 8.1:+6965469 | MS.gene24813:CDS |
| TCCATCCTGTAGTGAGTAAA+GGG | 0.439850 | 8.1:-6965381 | None:intergenic |
| TTCCAAGAGTTAGTATGAAT+CGG | 0.446733 | 8.1:-6965471 | None:intergenic |
| CATAAGAAATCATTGAAGTC+TGG | 0.456714 | 8.1:-6965726 | None:intergenic |
| TTTGTCTGATGCCTTCGAAA+TGG | 0.461961 | 8.1:-6965423 | None:intergenic |
| TGCTTCTGCTGTGGAAGGGA+GGG | 0.473215 | 8.1:-6965519 | None:intergenic |
| AAATCGTGCTAAACTATGTT+GGG | 0.473587 | 8.1:+6965443 | MS.gene24813:CDS |
| TGTCAAAATAAAATGATTTG+CGG | 0.475450 | 8.1:-6965555 | None:intergenic |
| ACATCTATCAGATTCCAAAC+TGG | 0.476477 | 8.1:-6965313 | None:intergenic |
| CAAATCGTGCTAAACTATGT+TGG | 0.476784 | 8.1:+6965442 | MS.gene24813:CDS |
| TTCCTATAGACATGAACAAA+AGG | 0.483981 | 8.1:-6965942 | None:intergenic |
| ATTCGTTTCAAGTTCTTCGA+TGG | 0.500169 | 8.1:+6965909 | MS.gene24813:CDS |
| TTCGTTTCAAGTTCTTCGAT+GGG | 0.505866 | 8.1:+6965910 | MS.gene24813:CDS |
| AACAACAATGCATTCCAGAT+TGG | 0.510902 | 8.1:+6965879 | MS.gene24813:CDS |
| ATCCTCCCTTTACTCACTAC+AGG | 0.520199 | 8.1:+6965376 | MS.gene24813:CDS |
| GTCCATCCTGTAGTGAGTAA+AGG | 0.525188 | 8.1:-6965382 | None:intergenic |
| CACTTTGACTCATTTGCAGA+TGG | 0.525652 | 8.1:+6965579 | MS.gene24813:CDS |
| GTGCTTCTGCTGTGGAAGGG+AGG | 0.535843 | 8.1:-6965520 | None:intergenic |
| TTTGTGCTTCTGCTGTGGAA+GGG | 0.552787 | 8.1:-6965523 | None:intergenic |
| GATTCATACTAACTCTTGGA+AGG | 0.611155 | 8.1:+6965473 | MS.gene24813:CDS |
| TCCCTTTACTCACTACAGGA+TGG | 0.611661 | 8.1:+6965380 | MS.gene24813:CDS |
| AAGAAATCATTGAAGTCTGG+TGG | 0.620565 | 8.1:-6965723 | None:intergenic |
| GAAGGGAGGGAATTCATCAG+TGG | 0.689324 | 8.1:-6965506 | None:intergenic |
| ATCCTGTAGTGAGTAAAGGG+AGG | 0.695428 | 8.1:-6965378 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTATTAAGTTTACAAAC+AGG | - | chr8.1:6965195-6965214 | None:intergenic | 15.0% |
| !! | TATTATTAAGTTTACAAACA+GGG | - | chr8.1:6965194-6965213 | None:intergenic | 15.0% |
| !!! | AAAATTGGTTTTTGTTAAAT+TGG | - | chr8.1:6965686-6965705 | None:intergenic | 15.0% |
| !! | ATACTAAGTTAAAAAATGGT+TGG | + | chr8.1:6965844-6965863 | MS.gene24813:CDS | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+TGG | + | chr8.1:6965840-6965859 | MS.gene24813:CDS | 20.0% |
| !! | TACTAAGTTAAAAAATGGTT+GGG | + | chr8.1:6965845-6965864 | MS.gene24813:CDS | 20.0% |
| !! | TGTCAAAATAAAATGATTTG+CGG | - | chr8.1:6965558-6965577 | None:intergenic | 20.0% |
| ! | AATATATACAAAACCTGATC+TGG | - | chr8.1:6965129-6965148 | None:intergenic | 25.0% |
| ! | AATGTAACTTTCAACAATTC+AGG | + | chr8.1:6965352-6965371 | MS.gene24813:CDS | 25.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | + | chr8.1:6965728-6965747 | MS.gene24813:CDS | 25.0% |
| !! | AAACCAATTTTGCAAATTAC+AGG | + | chr8.1:6965695-6965714 | MS.gene24813:intron | 25.0% |
| !! | AAATAAAATGATTTGCGGTT+CGG | - | chr8.1:6965553-6965572 | None:intergenic | 25.0% |
| !! | CGAATTCTTCAAGAAATTTT+GGG | - | chr8.1:6965282-6965301 | None:intergenic | 25.0% |
| !! | TAAATGTTGATTATGTGTAC+AGG | + | chr8.1:6965240-6965259 | MS.gene24813:intron | 25.0% |
| !! | TCGAATTCTTCAAGAAATTT+TGG | - | chr8.1:6965283-6965302 | None:intergenic | 25.0% |
| AAATCGTGCTAAACTATGTT+GGG | + | chr8.1:6965443-6965462 | MS.gene24813:CDS | 30.0% | |
| ACATAGTTTGGTTAGAAAAC+AGG | - | chr8.1:6965654-6965673 | None:intergenic | 30.0% | |
| ATCAGATAAAAAACAGCAGT+TGG | - | chr8.1:6965160-6965179 | None:intergenic | 30.0% | |
| CAGACTTCAATGATTTCTTA+TGG | + | chr8.1:6965727-6965746 | MS.gene24813:CDS | 30.0% | |
| CATAAGAAATCATTGAAGTC+TGG | - | chr8.1:6965729-6965748 | None:intergenic | 30.0% | |
| TGGAATCTGATAGATGTTAA+TGG | + | chr8.1:6965319-6965338 | MS.gene24813:CDS | 30.0% | |
| TGGTGAAAAAACACATAGTT+TGG | - | chr8.1:6965666-6965685 | None:intergenic | 30.0% | |
| TTCCAAGAGTTAGTATGAAT+CGG | - | chr8.1:6965474-6965493 | None:intergenic | 30.0% | |
| TTCCTATAGACATGAACAAA+AGG | - | chr8.1:6965945-6965964 | None:intergenic | 30.0% | |
| ! | AGAGATTTTTACCATTTCGA+AGG | + | chr8.1:6965412-6965431 | MS.gene24813:CDS | 30.0% |
| ! | GAACCTGTAATTTGCAAAAT+TGG | - | chr8.1:6965701-6965720 | None:intergenic | 30.0% |
| !!! | AAGAAATTTTGGGTTCACTT+CGG | - | chr8.1:6965272-6965291 | None:intergenic | 30.0% |
| AACAACAATGCATTCCAGAT+TGG | + | chr8.1:6965879-6965898 | MS.gene24813:CDS | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | - | chr8.1:6965726-6965745 | None:intergenic | 35.0% | |
| ACATCTATCAGATTCCAAAC+TGG | - | chr8.1:6965316-6965335 | None:intergenic | 35.0% | |
| CAAATCGTGCTAAACTATGT+TGG | + | chr8.1:6965442-6965461 | MS.gene24813:CDS | 35.0% | |
| GATTCATACTAACTCTTGGA+AGG | + | chr8.1:6965473-6965492 | MS.gene24813:CDS | 35.0% | |
| GATTTGCATTGCAGAGAAAA+AGG | - | chr8.1:6965770-6965789 | None:intergenic | 35.0% | |
| GCTGTAACAAACTCTTCATT+CGG | - | chr8.1:6965792-6965811 | None:intergenic | 35.0% | |
| TATTCAGCGATTCATTGCTT+TGG | - | chr8.1:6965827-6965846 | None:intergenic | 35.0% | |
| ! | CTCCTTTTGTTCATGTCTAT+AGG | + | chr8.1:6965940-6965959 | MS.gene24813:CDS | 35.0% |
| ! | GTACTCAAACGACAAGTTTT+GGG | + | chr8.1:6965081-6965100 | MS.gene24813:CDS | 35.0% |
| ! | TCGATGATTTGAAACCAGTT+TGG | + | chr8.1:6965299-6965318 | MS.gene24813:CDS | 35.0% |
| ! | TGTACTCAAACGACAAGTTT+TGG | + | chr8.1:6965080-6965099 | MS.gene24813:CDS | 35.0% |
| !! | ATTCGTTTCAAGTTCTTCGA+TGG | + | chr8.1:6965909-6965928 | MS.gene24813:CDS | 35.0% |
| !! | TTCGTTTCAAGTTCTTCGAT+GGG | + | chr8.1:6965910-6965929 | MS.gene24813:CDS | 35.0% |
| AACGAATAGTGTCTCCAATC+TGG | - | chr8.1:6965896-6965915 | None:intergenic | 40.0% | |
| AACTGTACATCTTCCAGATC+AGG | + | chr8.1:6965113-6965132 | MS.gene24813:CDS | 40.0% | |
| CACTTTGACTCATTTGCAGA+TGG | + | chr8.1:6965579-6965598 | MS.gene24813:CDS | 40.0% | |
| GATGGCAGAAAGATCTGAAT+TGG | + | chr8.1:6965597-6965616 | MS.gene24813:CDS | 40.0% | |
| TCCATCCTGTAGTGAGTAAA+GGG | - | chr8.1:6965384-6965403 | None:intergenic | 40.0% | |
| TTTGTCTGATGCCTTCGAAA+TGG | - | chr8.1:6965426-6965445 | None:intergenic | 40.0% | |
| ! | AGCCGATTCATACTAACTCT+TGG | + | chr8.1:6965469-6965488 | MS.gene24813:CDS | 40.0% |
| ATCCTCCCTTTACTCACTAC+AGG | + | chr8.1:6965376-6965395 | MS.gene24813:CDS | 45.0% | |
| ATCCTGTAGTGAGTAAAGGG+AGG | - | chr8.1:6965381-6965400 | None:intergenic | 45.0% | |
| GTCCATCCTGTAGTGAGTAA+AGG | - | chr8.1:6965385-6965404 | None:intergenic | 45.0% | |
| TCCCTTTACTCACTACAGGA+TGG | + | chr8.1:6965380-6965399 | MS.gene24813:CDS | 45.0% | |
| TTTGTGCTTCTGCTGTGGAA+GGG | - | chr8.1:6965526-6965545 | None:intergenic | 45.0% | |
| ! | TTTTGTGCTTCTGCTGTGGA+AGG | - | chr8.1:6965527-6965546 | None:intergenic | 45.0% |
| !!! | CTCAAACGACAAGTTTTGGG+AGG | + | chr8.1:6965084-6965103 | MS.gene24813:CDS | 45.0% |
| !!! | GTAGTTTTGTGCTTCTGCTG+TGG | - | chr8.1:6965531-6965550 | None:intergenic | 45.0% |
| GAAGGGAGGGAATTCATCAG+TGG | - | chr8.1:6965509-6965528 | None:intergenic | 50.0% | |
| TGCTTCTGCTGTGGAAGGGA+GGG | - | chr8.1:6965522-6965541 | None:intergenic | 55.0% | |
| GTGCTTCTGCTGTGGAAGGG+AGG | - | chr8.1:6965523-6965542 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 6965063 | 6965974 | 6965063 | ID=MS.gene24813 |
| chr8.1 | mRNA | 6965063 | 6965974 | 6965063 | ID=MS.gene24813.t1;Parent=MS.gene24813 |
| chr8.1 | exon | 6965063 | 6965134 | 6965063 | ID=MS.gene24813.t1.exon1;Parent=MS.gene24813.t1 |
| chr8.1 | CDS | 6965063 | 6965134 | 6965063 | ID=cds.MS.gene24813.t1;Parent=MS.gene24813.t1 |
| chr8.1 | exon | 6965262 | 6965618 | 6965262 | ID=MS.gene24813.t1.exon2;Parent=MS.gene24813.t1 |
| chr8.1 | CDS | 6965262 | 6965618 | 6965262 | ID=cds.MS.gene24813.t1;Parent=MS.gene24813.t1 |
| chr8.1 | exon | 6965717 | 6965974 | 6965717 | ID=MS.gene24813.t1.exon3;Parent=MS.gene24813.t1 |
| chr8.1 | CDS | 6965717 | 6965974 | 6965717 | ID=cds.MS.gene24813.t1;Parent=MS.gene24813.t1 |
| Gene Sequence |
| Protein sequence |
