AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24983

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24983.t1 MTR_4g014070 71.579 190 27 4 69 239 563 744 9.59e-68 223
MS.gene24983.t1 MTR_5g083480 60.656 183 59 3 69 239 558 739 3.09e-59 200
MS.gene24983.t1 MTR_5g083480 60.656 183 59 3 69 239 555 736 3.56e-59 200
MS.gene24983.t1 MTR_4g113100 39.779 181 80 7 69 229 407 578 2.93e-26 107
MS.gene24983.t1 MTR_8g107470 38.830 188 72 8 69 229 435 606 4.92e-26 106
MS.gene24983.t1 MTR_5g009660 37.000 200 90 8 66 241 357 544 1.38e-25 105
MS.gene24983.t1 MTR_8g099195 37.500 200 89 8 66 241 380 567 3.07e-25 104
MS.gene24983.t1 MTR_6g470960 40.642 187 70 9 69 229 438 609 1.08e-23 99.8
MS.gene24983.t1 MTR_3g062500 39.106 179 85 7 69 229 425 597 1.23e-23 99.8
MS.gene24983.t1 MTR_3g062500 39.106 179 85 7 69 229 415 587 1.34e-23 99.4
MS.gene24983.t1 MTR_3g090665 37.912 182 92 6 65 229 111 288 4.17e-23 96.3
MS.gene24983.t1 MTR_5g068210 35.859 198 91 7 69 241 373 559 7.33e-23 97.4
MS.gene24983.t1 MTR_5g078080 36.667 180 87 7 69 229 400 571 1.03e-22 97.1
MS.gene24983.t1 MTR_5g075630 40.230 174 90 7 69 229 416 588 1.06e-22 97.1
MS.gene24983.t1 MTR_5g055470 37.245 196 97 9 66 243 397 584 1.80e-22 96.3
MS.gene24983.t1 MTR_1g061590 37.313 201 76 9 66 229 420 607 3.53e-22 95.5
MS.gene24983.t1 MTR_7g073710 34.043 188 88 6 66 229 392 567 5.22e-22 94.7
MS.gene24983.t1 MTR_1g028890 35.052 194 89 8 69 241 351 528 1.20e-21 93.6
MS.gene24983.t1 MTR_4g014210 53.763 93 12 2 5 70 34 122 1.20e-21 89.0
MS.gene24983.t1 MTR_1g110280 36.216 185 96 6 66 229 415 598 6.78e-21 91.7
MS.gene24983.t1 MTR_3g093710 35.079 191 99 6 69 241 403 586 9.44e-21 91.3
MS.gene24983.t1 MTR_7g103440 34.826 201 81 9 66 229 433 620 4.75e-20 89.4
MS.gene24983.t1 MTR_3g090660 36.872 179 90 7 69 229 381 554 1.44e-19 87.8
MS.gene24983.t1 MTR_7g059225 31.604 212 98 7 69 246 441 639 1.22e-18 85.1
MS.gene24983.t1 MTR_4g014130 100.000 36 0 0 204 239 1 36 2.24e-18 77.4
MS.gene24983.t1 MTR_2g023150 33.529 170 90 5 70 230 56 211 3.97e-18 81.3
MS.gene24983.t1 MTR_1g009270 34.021 194 108 7 70 246 404 594 2.67e-17 81.3
MS.gene24983.t1 MTR_2g030380 34.975 203 105 10 53 230 808 1008 5.06e-17 80.5
MS.gene24983.t1 MTR_4g014240 52.688 93 17 1 5 70 905 997 5.50e-17 80.5
MS.gene24983.t1 MTR_2g030380 34.975 203 105 10 53 230 826 1026 5.58e-17 80.5
MS.gene24983.t1 MTR_2g070020 31.183 186 112 5 76 245 877 1062 6.30e-17 80.1
MS.gene24983.t1 MTR_1g047670 30.846 201 116 7 63 245 871 1066 7.74e-16 77.0
MS.gene24983.t1 MTR_2g072520 33.520 179 101 6 66 227 81 258 5.22e-15 73.2
MS.gene24983.t1 MTR_4g088320 30.952 168 99 6 76 227 764 930 1.61e-14 73.2
MS.gene24983.t1 MTR_3g078250 30.588 170 109 4 70 233 430 596 1.67e-14 72.8
MS.gene24983.t1 MTR_5g094380 31.937 191 105 7 72 246 330 511 1.79e-14 72.8
MS.gene24983.t1 MTR_8g087420 30.769 195 113 8 70 246 401 591 3.18e-14 72.0
MS.gene24983.t1 MTR_1g100787 29.570 186 97 3 65 224 1048 1225 3.56e-14 72.0
MS.gene24983.t1 MTR_6g093050 31.122 196 115 7 70 246 402 596 3.93e-14 71.6
MS.gene24983.t1 MTR_2g090710 30.952 168 98 5 77 227 761 927 5.72e-14 71.6
MS.gene24983.t1 MTR_2g008740 31.250 176 100 5 70 227 174 346 5.85e-14 70.9
MS.gene24983.t1 MTR_7g084220 27.320 194 95 4 70 232 849 1027 9.80e-14 70.9
MS.gene24983.t1 MTR_5g087780 29.648 199 112 7 72 243 773 970 1.67e-13 70.1
MS.gene24983.t1 MTR_4g107620 27.895 190 117 6 72 245 377 562 1.68e-13 70.1
MS.gene24983.t1 MTR_4g014320 42.857 112 31 4 5 90 895 999 2.61e-13 69.7
MS.gene24983.t1 MTR_3g095100 29.381 194 103 6 72 240 937 1121 7.26e-13 68.2
MS.gene24983.t1 MTR_5g021670 31.875 160 89 6 69 210 894 1051 8.93e-13 68.2
MS.gene24983.t1 MTR_1g027670 28.409 176 93 4 80 230 123 290 1.16e-12 67.0
MS.gene24983.t1 MTR_7g063010 31.138 167 98 3 80 230 410 575 1.19e-12 67.4
MS.gene24983.t1 MTR_3g449390 30.682 176 85 7 72 222 773 936 1.22e-12 67.4
MS.gene24983.t1 MTR_6g069030 24.747 198 96 7 72 231 390 572 1.69e-12 67.0
MS.gene24983.t1 MTR_2g105900 30.693 202 107 9 70 246 404 597 3.42e-12 66.2
MS.gene24983.t1 MTR_1g082580 31.250 144 78 4 72 197 213 353 5.45e-12 65.1
MS.gene24983.t1 MTR_7g007620 31.387 137 80 3 76 199 511 646 6.19e-12 65.5
MS.gene24983.t1 MTR_2g042710 28.877 187 117 6 70 241 415 600 6.53e-12 65.1
MS.gene24983.t1 MTR_8g047160 33.333 138 76 4 76 199 422 557 9.10e-12 64.7
MS.gene24983.t1 MTR_0491s0030 34.532 139 73 5 76 199 595 730 1.12e-11 64.7
MS.gene24983.t1 MTR_1g010250 29.825 171 99 5 77 231 363 528 1.24e-11 64.3
MS.gene24983.t1 MTR_5g014700 31.073 177 86 6 77 227 761 927 1.28e-11 64.7
MS.gene24983.t1 MTR_8g047230 34.532 139 73 5 76 199 766 901 1.40e-11 64.3
MS.gene24983.t1 MTR_2g010470 30.481 187 111 8 76 246 761 944 1.86e-11 63.9
MS.gene24983.t1 MTR_8g046290 31.481 162 93 4 70 214 129 289 2.39e-11 63.2
MS.gene24983.t1 MTR_1g010220 29.240 171 100 5 77 231 482 647 3.84e-11 63.2
MS.gene24983.t1 MTR_1g010240 30.370 135 80 3 77 197 404 538 4.93e-11 62.8
MS.gene24983.t1 MTR_1g010260 30.370 135 80 3 77 197 490 624 6.04e-11 62.4
MS.gene24983.t1 MTR_3g047890 28.161 174 104 5 79 233 250 421 7.29e-11 62.0
MS.gene24983.t1 MTR_1g079520 30.921 152 89 3 80 216 841 991 8.99e-11 62.0
MS.gene24983.t1 MTR_3g094710 41.304 92 48 2 142 227 899 990 9.20e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24983.t1 AT3G51740 57.368 190 54 4 69 239 597 778 1.43e-57 196
MS.gene24983.t1 AT2G26730 42.938 177 79 7 69 229 410 580 1.85e-27 110
MS.gene24983.t1 AT1G68400 39.665 179 92 6 66 229 420 597 1.49e-24 102
MS.gene24983.t1 AT4G23740 37.234 188 83 6 66 229 393 569 2.99e-24 101
MS.gene24983.t1 AT4G23740 37.234 188 83 6 66 229 393 569 2.99e-24 101
MS.gene24983.t1 AT3G08680 34.555 191 102 5 69 241 401 586 6.19e-23 97.8
MS.gene24983.t1 AT3G08680 34.555 191 102 5 69 241 401 586 6.19e-23 97.8
MS.gene24983.t1 AT5G05160 34.857 175 94 6 70 229 408 577 8.73e-23 97.1
MS.gene24983.t1 AT5G58300 39.106 179 85 7 69 229 418 590 3.05e-22 95.5
MS.gene24983.t1 AT5G58300 39.106 179 85 7 69 229 418 590 3.05e-22 95.5
MS.gene24983.t1 AT5G58300 39.106 179 85 7 69 229 418 590 3.05e-22 95.5
MS.gene24983.t1 AT3G17840 38.418 177 87 7 69 228 424 595 6.94e-22 94.7
MS.gene24983.t1 AT1G48480 37.288 177 89 7 69 228 430 601 7.60e-22 94.4
MS.gene24983.t1 AT5G41680 35.000 180 91 6 67 228 116 287 1.97e-20 89.0
MS.gene24983.t1 AT3G24660 31.500 200 115 5 69 246 433 632 2.23e-20 90.1
MS.gene24983.t1 AT2G36570 37.129 202 79 9 66 229 415 606 4.75e-20 89.4
MS.gene24983.t1 AT5G16590 35.057 174 96 6 70 229 403 573 3.06e-19 87.0
MS.gene24983.t1 AT1G64210 31.122 196 110 7 66 241 363 553 6.47e-19 85.9
MS.gene24983.t1 AT3G02880 31.122 196 98 6 70 241 405 587 7.46e-19 85.9
MS.gene24983.t1 AT5G41680 33.168 202 91 7 67 228 116 313 2.67e-18 83.2
MS.gene24983.t1 AT5G53320 31.214 173 93 4 72 229 369 530 1.10e-17 82.4
MS.gene24983.t1 AT5G24100 30.882 204 109 6 60 241 390 583 1.90e-17 81.6
MS.gene24983.t1 AT4G31250 32.308 195 113 8 70 246 427 620 6.48e-17 80.1
MS.gene24983.t1 AT4G20940 34.054 185 97 8 69 230 814 996 2.03e-15 75.9
MS.gene24983.t1 AT1G17230 30.653 199 119 5 63 245 857 1052 3.53e-15 75.1
MS.gene24983.t1 AT3G20190 33.673 196 109 8 70 246 244 437 3.86e-15 74.7
MS.gene24983.t1 AT1G17230 30.653 199 119 5 63 245 857 1052 4.05e-15 75.1
MS.gene24983.t1 AT2G33170 30.952 168 100 4 72 224 890 1056 5.54e-15 74.7
MS.gene24983.t1 AT2G33170 30.952 168 100 4 72 224 890 1056 5.54e-15 74.7
MS.gene24983.t1 AT2G07040 33.333 198 107 8 70 246 399 592 5.62e-15 74.3
MS.gene24983.t1 AT3G20190 33.673 196 109 8 70 246 433 626 5.82e-15 74.3
MS.gene24983.t1 AT3G49670 32.203 177 84 6 77 227 759 925 3.55e-14 72.0
MS.gene24983.t1 AT5G62710 27.528 178 96 4 72 224 374 543 4.81e-14 71.6
MS.gene24983.t1 AT5G62710 26.842 190 103 5 60 224 331 509 5.17e-14 71.6
MS.gene24983.t1 AT5G63930 30.108 186 95 6 80 241 879 1053 1.01e-13 70.9
MS.gene24983.t1 AT5G63930 30.108 186 95 6 80 241 815 989 1.04e-13 70.9
MS.gene24983.t1 AT4G39400 29.381 194 103 6 72 240 945 1129 5.44e-13 68.6
MS.gene24983.t1 AT4G36180 30.539 167 94 7 60 210 896 1056 6.50e-13 68.6
MS.gene24983.t1 AT2G27060 31.351 185 96 6 66 224 788 967 7.84e-13 68.2
MS.gene24983.t1 AT1G08590 29.630 189 107 6 72 239 790 973 9.92e-13 67.8
MS.gene24983.t1 AT5G59270 28.177 181 96 4 72 227 430 601 1.11e-12 67.8
MS.gene24983.t1 AT1G75640 33.333 168 89 8 60 210 894 1055 1.13e-12 67.8
MS.gene24983.t1 AT5G59270 28.177 181 96 4 72 227 414 585 1.17e-12 67.4
MS.gene24983.t1 AT5G59270 28.177 181 96 4 72 227 411 582 1.20e-12 67.4
MS.gene24983.t1 AT2G27060 31.351 185 96 6 66 224 695 874 1.52e-12 67.4
MS.gene24983.t1 AT1G69270 28.421 190 111 7 72 241 323 507 1.80e-12 67.0
MS.gene24983.t1 AT5G65700 32.278 158 73 5 77 210 763 910 5.87e-12 65.5
MS.gene24983.t1 AT5G65700 32.278 158 73 5 77 210 763 910 5.87e-12 65.5
MS.gene24983.t1 AT3G02130 36.190 105 60 2 142 241 1016 1118 7.67e-12 65.1
MS.gene24983.t1 AT5G35390 31.250 192 116 7 70 246 418 608 7.85e-12 65.1
MS.gene24983.t1 AT1G70110 30.675 163 98 5 72 221 407 567 8.24e-12 65.1
MS.gene24983.t1 AT3G02130 36.190 105 60 2 142 241 999 1101 8.39e-12 65.1
MS.gene24983.t1 AT1G70130 31.690 142 83 4 72 200 397 537 9.26e-12 64.7
MS.gene24983.t1 AT2G02220 32.044 181 93 8 66 224 793 965 1.51e-11 64.3
MS.gene24983.t1 AT5G07620 24.631 203 117 4 70 246 140 332 4.21e-11 62.4
MS.gene24983.t1 AT4G02420 29.655 145 86 4 72 202 413 555 6.84e-11 62.4
MS.gene24983.t1 AT1G50610 33.520 179 91 8 70 227 436 607 8.77e-11 62.0

Find 55 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCCTCGGTGTTATCCTTT+TGG 0.248528 4.1:-82239474 MS.gene24983:CDS
TTCCATGCACTATATTCTTT+TGG 0.283746 4.1:+82239644 None:intergenic
ACAAGAAGCTTCTCTTCTTT+AGG 0.291032 4.1:+82240223 None:intergenic
GACAAATGAAGTGTTTGATT+TGG 0.323614 4.1:-82239366 MS.gene24983:CDS
TTCTTTGTCTTTGAAAGCTC+TGG 0.341174 4.1:+82239521 None:intergenic
GAAGCTTCTGTAACTGAAAT+CGG 0.348061 4.1:-82239734 MS.gene24983:intron
ATGCCATTTGTTGGTTCTCC+TGG 0.362960 4.1:+82239431 None:intergenic
CTGAACAATGCAAGGAGAAC+AGG 0.403237 4.1:-82240900 None:intergenic
CATCCATTGTTAAAGAAGAA+TGG 0.404547 4.1:-82239388 MS.gene24983:CDS
TTTAAGAAGTTCATCTCCTA+TGG 0.410729 4.1:+82239322 None:intergenic
GAACCTCGTTCCCCTTTGCT+TGG 0.413180 4.1:-82240696 MS.gene24983:CDS
GGCAAGTCCATGCCATTTGT+TGG 0.425256 4.1:+82239422 None:intergenic
GATTTGTACTCGGTGGGAAC+TGG 0.426192 4.1:-82240662 MS.gene24983:CDS
ACAAGGATGAAAATAGCAAT+TGG 0.434911 4.1:-82239697 MS.gene24983:CDS
CACCTCAAGCAACATACAAT+TGG 0.458004 4.1:-82239617 MS.gene24983:CDS
TGTCCATTCTTCTTTAACAA+TGG 0.458354 4.1:+82239385 None:intergenic
ACAATGGATGCAACATATTG+AGG 0.461356 4.1:+82239401 None:intergenic
CGAAAATGAAGCCAAGCAAA+GGG 0.468116 4.1:+82240685 None:intergenic
ATTGCTACAGCAGGAAGTCT+TGG 0.477792 4.1:-82239554 MS.gene24983:CDS
AATGCAAGGAGAACAGGAAA+AGG 0.481384 4.1:-82240894 MS.gene24983:CDS
TTCAATTTCTGAACAATGCA+AGG 0.495733 4.1:-82240908 None:intergenic
ATCACTATCTGTGATGTTGA+GGG 0.495900 4.1:-82240612 MS.gene24983:CDS
ATCTGTGATGTTGAGGGGCA+AGG 0.519133 4.1:-82240606 MS.gene24983:intron
CATCACTATCTGTGATGTTG+AGG 0.520950 4.1:-82240613 MS.gene24983:CDS
CCGAAAATGAAGCCAAGCAA+AGG 0.523710 4.1:+82240684 None:intergenic
AGAAGTTCATCTCCTATGGT+TGG 0.529629 4.1:+82239326 None:intergenic
TCCTGCTGTAGCAATGATGT+TGG 0.533607 4.1:+82239562 None:intergenic
GATTTGGAACTCATGAGGGA+TGG 0.537502 4.1:-82239350 MS.gene24983:CDS
CCACCAGGAGAACCAACAAA+TGG 0.539629 4.1:-82239434 MS.gene24983:CDS
ACCAACATCATTGCTACAGC+AGG 0.542435 4.1:-82239563 MS.gene24983:CDS
CCATTTGTTGGTTCTCCTGG+TGG 0.548920 4.1:+82239434 None:intergenic
TACCAGTTAAGAGTTCCAAA+AGG 0.550195 4.1:+82239459 None:intergenic
TGTAGCAATGATGTTGGTGT+TGG 0.550967 4.1:+82239568 None:intergenic
ATGCAAGGAGAACAGGAAAA+GGG 0.561345 4.1:-82240893 MS.gene24983:CDS
AACCAAAAGAATATAGTGCA+TGG 0.563084 4.1:-82239646 MS.gene24983:CDS
CTCTTAACTGGTAAACCACC+AGG 0.563734 4.1:-82239449 MS.gene24983:CDS
GTCTTTGATTACATGAGTAG+AGG 0.565999 4.1:-82240202 MS.gene24983:CDS
CTACTTAGTGCTTACTGTGA+AGG 0.568616 4.1:+82240164 None:intergenic
AAAACCGATGTTTACAGCCT+CGG 0.571110 4.1:-82239488 MS.gene24983:CDS
TGTTTGATTTGGAACTCATG+AGG 0.576156 4.1:-82239355 MS.gene24983:CDS
AGGAGAACCAACAAATGGCA+TGG 0.605960 4.1:-82239429 MS.gene24983:CDS
ATGAGGGATGGACCAACCAT+AGG 0.609052 4.1:-82239338 MS.gene24983:CDS
ATAGCAATTGGAATCGCCAA+TGG 0.612433 4.1:-82239685 MS.gene24983:CDS
GTGGAGGCAAGAGAGACCAT+TGG 0.612548 4.1:+82239669 None:intergenic
CGGTTTCGAATGGCCCAACA+AGG 0.612620 4.1:-82239714 MS.gene24983:CDS
TACTGTGAAGGAATGATGCT+AGG 0.621800 4.1:+82240176 None:intergenic
GTTTGATTTGGAACTCATGA+GGG 0.628775 4.1:-82239354 MS.gene24983:CDS
GACCAATTGTATGTTGCTTG+AGG 0.629446 4.1:+82239615 None:intergenic
TAACTGAAATCGGTTTCGAA+TGG 0.632676 4.1:-82239724 MS.gene24983:CDS
TCACTATCTGTGATGTTGAG+GGG 0.653665 4.1:-82240611 MS.gene24983:CDS
AACACCGAGGCTGTAAACAT+CGG 0.661124 4.1:+82239484 None:intergenic
AAGCCAAGCAAAGGGGAACG+AGG 0.661699 4.1:+82240693 None:intergenic
AAGATAGATAAGAGTCAATG+TGG 0.664029 4.1:+82240724 None:intergenic
GAAAATGAAGCCAAGCAAAG+GGG 0.713672 4.1:+82240686 None:intergenic
GTTCCAAAAGGATAACACCG+AGG 0.781912 4.1:+82239471 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAACAAGTTTTAAAATCA+AGG - chr4.1:82239711-82239730 MS.gene24983:CDS 15.0%
!!! TCAACTTTGTATTATAATTT+TGG - chr4.1:82240158-82240177 MS.gene24983:intron 15.0%
!!! TTTAAAACTTGTTATAACTT+TGG + chr4.1:82239707-82239726 None:intergenic 15.0%
!! TTATGGACATTTATTAATGT+TGG - chr4.1:82240272-82240291 MS.gene24983:intron 20.0%
!!! AATGTTTTCTAAGTAAGTTT+TGG + chr4.1:82240376-82240395 None:intergenic 20.0%
! AAATATTTCAAGAGGAATTG+AGG - chr4.1:82239873-82239892 MS.gene24983:intron 25.0%
! AGTATAACAATCATATGATC+TGG + chr4.1:82240208-82240227 None:intergenic 25.0%
! CATATGTATGAATAACTTCT+AGG + chr4.1:82239763-82239782 None:intergenic 25.0%
! CATTTATTAATGTTGGAACA+TGG - chr4.1:82240279-82240298 MS.gene24983:intron 25.0%
! TAGATATGAAGAGTTCAAAA+TGG + chr4.1:82239626-82239645 None:intergenic 25.0%
! TCTACTTGAAATATTTCAAG+AGG - chr4.1:82239865-82239884 MS.gene24983:intron 25.0%
!! GAAATATTTCAAGTAGAATC+AGG + chr4.1:82239862-82239881 None:intergenic 25.0%
!! TTATAACTTTGGCGTTTTAT+TGG + chr4.1:82239696-82239715 None:intergenic 25.0%
AACCAAAAGAATATAGTGCA+TGG - chr4.1:82240548-82240567 MS.gene24983:intron 30.0%
AAGATAGATAAGAGTCAATG+TGG + chr4.1:82239473-82239492 None:intergenic 30.0%
ACAAGGATGAAAATAGCAAT+TGG - chr4.1:82240497-82240516 MS.gene24983:intron 30.0%
ACTATCCAATCTTGATACAA+AGG - chr4.1:82240302-82240321 MS.gene24983:intron 30.0%
CATCCATTGTTAAAGAAGAA+TGG - chr4.1:82240806-82240825 MS.gene24983:intron 30.0%
TGTCCATTCTTCTTTAACAA+TGG + chr4.1:82240812-82240831 None:intergenic 30.0%
TTTAAGAAGTTCATCTCCTA+TGG + chr4.1:82240875-82240894 None:intergenic 30.0%
! GACAAATGAAGTGTTTGATT+TGG - chr4.1:82240828-82240847 MS.gene24983:intron 30.0%
! TTCATTTTCGGATTTGTACT+CGG - chr4.1:82239522-82239541 MS.gene24983:CDS 30.0%
! TTCCATGCACTATATTCTTT+TGG + chr4.1:82240553-82240572 None:intergenic 30.0%
! TTGTTGATTATATGTTCCAG+AGG - chr4.1:82240114-82240133 MS.gene24983:intron 30.0%
!!! AGTAAGTTTTGGTTTACTTG+CGG + chr4.1:82240365-82240384 None:intergenic 30.0%
!!! ATTGCTATTTTCATCCTTGT+TGG + chr4.1:82240497-82240516 None:intergenic 30.0%
!!! TTGCTATTTTCATCCTTGTT+GGG + chr4.1:82240496-82240515 None:intergenic 30.0%
ACAAGAAGCTTCTCTTCTTT+AGG + chr4.1:82239974-82239993 None:intergenic 35.0%
ACAATGGATGCAACATATTG+AGG + chr4.1:82240796-82240815 None:intergenic 35.0%
ACATATGCATGCTGCTAAAA+GGG - chr4.1:82239776-82239795 MS.gene24983:intron 35.0%
AGCATTAAACACAGTATTCG+AGG + chr4.1:82239366-82239385 None:intergenic 35.0%
AGGTTCAAGATAGTAAGCTT+GGG + chr4.1:82239954-82239973 None:intergenic 35.0%
ATCACTATCTGTGATGTTGA+GGG - chr4.1:82239582-82239601 MS.gene24983:CDS 35.0%
CCATAACGATAATCCAAAGA+TGG + chr4.1:82240258-82240277 None:intergenic 35.0%
CCATCTTTGGATTATCGTTA+TGG - chr4.1:82240255-82240274 MS.gene24983:intron 35.0%
CTAGACCTTTGTATCAAGAT+TGG + chr4.1:82240310-82240329 None:intergenic 35.0%
GAAGACAGATATTCCATCTT+TGG - chr4.1:82240242-82240261 MS.gene24983:intron 35.0%
GAAGCTTCTGTAACTGAAAT+CGG - chr4.1:82240460-82240479 MS.gene24983:intron 35.0%
GACATGACTGGATTCAATAT+TGG + chr4.1:82239658-82239677 None:intergenic 35.0%
GCATTAAACACAGTATTCGA+GGG + chr4.1:82239365-82239384 None:intergenic 35.0%
GTCTTTGATTACATGAGTAG+AGG - chr4.1:82239992-82240011 MS.gene24983:intron 35.0%
GTGTGCGAATTGCTTAAAAT+TGG - chr4.1:82239328-82239347 MS.gene24983:CDS 35.0%
TACATATGCATGCTGCTAAA+AGG - chr4.1:82239775-82239794 MS.gene24983:intron 35.0%
TACCAGTTAAGAGTTCCAAA+AGG + chr4.1:82240738-82240757 None:intergenic 35.0%
TAGATCAGAAAAGACATGAC+TGG + chr4.1:82239670-82239689 None:intergenic 35.0%
TAGGTTCAAGATAGTAAGCT+TGG + chr4.1:82239955-82239974 None:intergenic 35.0%
! AATTTTGGACAATCCGATCT+TGG - chr4.1:82240173-82240192 MS.gene24983:intron 35.0%
! TAACTGAAATCGGTTTCGAA+TGG - chr4.1:82240470-82240489 MS.gene24983:intron 35.0%
! TTCTTTGTCTTTGAAAGCTC+TGG + chr4.1:82240676-82240695 None:intergenic 35.0%
!! CTATATTCTTTTGGTTGTGG+AGG + chr4.1:82240544-82240563 None:intergenic 35.0%
!! GCACTATATTCTTTTGGTTG+TGG + chr4.1:82240547-82240566 None:intergenic 35.0%
!! GTTTGATTTGGAACTCATGA+GGG - chr4.1:82240840-82240859 MS.gene24983:intron 35.0%
!! TGAATAACTTCTAGGATGCA+TGG + chr4.1:82239755-82239774 None:intergenic 35.0%
!! TGTTTGATTTGGAACTCATG+AGG - chr4.1:82240839-82240858 MS.gene24983:intron 35.0%
!!! ATCCTTTTGGAACTCTTAAC+TGG - chr4.1:82240733-82240752 MS.gene24983:CDS 35.0%
!!! TAAACATCGGTTTTTGTCGT+AGG + chr4.1:82240700-82240719 None:intergenic 35.0%
AAAACCGATGTTTACAGCCT+CGG - chr4.1:82240706-82240725 MS.gene24983:CDS 40.0%
AATCACGCAATCATGTGATC+TGG + chr4.1:82240336-82240355 None:intergenic 40.0%
ACAATCCGATCTTGGTTCAA+TGG - chr4.1:82240181-82240200 MS.gene24983:CDS 40.0%
ACTGAAACTGTGATATGCTG+AGG + chr4.1:82239824-82239843 None:intergenic 40.0%
AGAAGTTCATCTCCTATGGT+TGG + chr4.1:82240871-82240890 None:intergenic 40.0%
ATAGCAATTGGAATCGCCAA+TGG - chr4.1:82240509-82240528 MS.gene24983:intron 40.0%
ATGCAAGGAGAACAGGAAAA+GGG - chr4.1:82239301-82239320 MS.gene24983:CDS 40.0%
CACCTCAAGCAACATACAAT+TGG - chr4.1:82240577-82240596 MS.gene24983:intron 40.0%
CATCACTATCTGTGATGTTG+AGG - chr4.1:82239581-82239600 MS.gene24983:CDS 40.0%
CGAAAATGAAGCCAAGCAAA+GGG + chr4.1:82239512-82239531 None:intergenic 40.0%
CTACTTAGTGCTTACTGTGA+AGG + chr4.1:82240033-82240052 None:intergenic 40.0%
CTGGATTCAATATTGGCAGA+TGG + chr4.1:82239651-82239670 None:intergenic 40.0%
GAAAATGAAGCCAAGCAAAG+GGG + chr4.1:82239511-82239530 None:intergenic 40.0%
TACTGTGAAGGAATGATGCT+AGG + chr4.1:82240021-82240040 None:intergenic 40.0%
TCACTATCTGTGATGTTGAG+GGG - chr4.1:82239583-82239602 MS.gene24983:CDS 40.0%
TCCAGAGGATCATGATTAGA+AGG - chr4.1:82240129-82240148 MS.gene24983:intron 40.0%
TGTAGCAATGATGTTGGTGT+TGG + chr4.1:82240629-82240648 None:intergenic 40.0%
! AGGGTACGGTGTTAACATAA+TGG - chr4.1:82239795-82239814 MS.gene24983:intron 40.0%
! ATTTTCGGATTTGTACTCGG+TGG - chr4.1:82239525-82239544 MS.gene24983:CDS 40.0%
! CCTTTGCTTGGCTTCATTTT+CGG - chr4.1:82239510-82239529 MS.gene24983:CDS 40.0%
! GACCAATTGTATGTTGCTTG+AGG + chr4.1:82240582-82240601 None:intergenic 40.0%
! TTTTCGGATTTGTACTCGGT+GGG - chr4.1:82239526-82239545 MS.gene24983:CDS 40.0%
!!! GTTTTGGTTTACTTGCGGTT+AGG + chr4.1:82240360-82240379 None:intergenic 40.0%
AACACCGAGGCTGTAAACAT+CGG + chr4.1:82240713-82240732 None:intergenic 45.0%
ACCAACATCATTGCTACAGC+AGG - chr4.1:82240631-82240650 MS.gene24983:CDS 45.0%
AGGAGAACCAACAAATGGCA+TGG - chr4.1:82240765-82240784 MS.gene24983:CDS 45.0%
ATTGCTACAGCAGGAAGTCT+TGG - chr4.1:82240640-82240659 MS.gene24983:CDS 45.0%
CCGAAAATGAAGCCAAGCAA+AGG + chr4.1:82239513-82239532 None:intergenic 45.0%
CTCTTAACTGGTAAACCACC+AGG - chr4.1:82240745-82240764 MS.gene24983:CDS 45.0%
CTGGACCATTGAACCAAGAT+CGG + chr4.1:82240189-82240208 None:intergenic 45.0%
GCCTTCTAATCATGATCCTC+TGG + chr4.1:82240133-82240152 None:intergenic 45.0%
GTTCCAAAAGGATAACACCG+AGG + chr4.1:82240726-82240745 None:intergenic 45.0%
TCCTGCTGTAGCAATGATGT+TGG + chr4.1:82240635-82240654 None:intergenic 45.0%
TGCATGCTGCTAAAAGGGTA+CGG - chr4.1:82239781-82239800 MS.gene24983:intron 45.0%
! GATTTGGAACTCATGAGGGA+TGG - chr4.1:82240844-82240863 MS.gene24983:intron 45.0%
!! ATGCCATTTGTTGGTTCTCC+TGG + chr4.1:82240766-82240785 None:intergenic 45.0%
ATCTGTGATGTTGAGGGGCA+AGG - chr4.1:82239588-82239607 MS.gene24983:CDS 50.0%
ATGAGGGATGGACCAACCAT+AGG - chr4.1:82240856-82240875 MS.gene24983:intron 50.0%
CCACCAGGAGAACCAACAAA+TGG - chr4.1:82240760-82240779 MS.gene24983:CDS 50.0%
TGTTGAGGGGCAAGGTAAAG+AGG - chr4.1:82239596-82239615 MS.gene24983:CDS 50.0%
! GATTTGTACTCGGTGGGAAC+TGG - chr4.1:82239532-82239551 MS.gene24983:CDS 50.0%
! GGCAAGTCCATGCCATTTGT+TGG + chr4.1:82240775-82240794 None:intergenic 50.0%
!! CCATTTGTTGGTTCTCCTGG+TGG + chr4.1:82240763-82240782 None:intergenic 50.0%
!!! CAGCCTCGGTGTTATCCTTT+TGG - chr4.1:82240720-82240739 MS.gene24983:CDS 50.0%
AAGCCAAGCAAAGGGGAACG+AGG + chr4.1:82239504-82239523 None:intergenic 55.0%
CGGTTTCGAATGGCCCAACA+AGG - chr4.1:82240480-82240499 MS.gene24983:intron 55.0%
GAACCTCGTTCCCCTTTGCT+TGG - chr4.1:82239498-82239517 MS.gene24983:CDS 55.0%
GTGGAGGCAAGAGAGACCAT+TGG + chr4.1:82240528-82240547 None:intergenic 55.0%
TGAGGGGCAAGGTAAAGAGG+AGG - chr4.1:82239599-82239618 MS.gene24983:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 82239301 82240915 82239301 ID=MS.gene24983
chr4.1 mRNA 82239301 82240915 82239301 ID=MS.gene24983.t1;Parent=MS.gene24983
chr4.1 exon 82240891 82240915 82240891 ID=MS.gene24983.t1.exon1;Parent=MS.gene24983.t1
chr4.1 CDS 82240891 82240915 82240891 ID=cds.MS.gene24983.t1;Parent=MS.gene24983.t1
chr4.1 exon 82240607 82240789 82240607 ID=MS.gene24983.t1.exon2;Parent=MS.gene24983.t1
chr4.1 CDS 82240607 82240789 82240607 ID=cds.MS.gene24983.t1;Parent=MS.gene24983.t1
chr4.1 exon 82240179 82240259 82240179 ID=MS.gene24983.t1.exon3;Parent=MS.gene24983.t1
chr4.1 CDS 82240179 82240259 82240179 ID=cds.MS.gene24983.t1;Parent=MS.gene24983.t1
chr4.1 exon 82239301 82239752 82239301 ID=MS.gene24983.t1.exon4;Parent=MS.gene24983.t1
chr4.1 CDS 82239301 82239752 82239301 ID=cds.MS.gene24983.t1;Parent=MS.gene24983.t1
Gene Sequence

>MS.gene24983

ATGCAAGGAGAACAGGAAAAGGGTTGTGTGTGCGAATTGCTTAAAATTGGATGAACATTCTCCCTCGAATACTGTGTTTAATGCTCAAATAAACACGTATGAAATTTGCATATCAACATGTATCAGCTTCAGAACACGATTTTCACAATAAGTTCAATGATTATAACGACCACATTGACTCTTATCTATCTTCTTCTGAACCTCGTTCCCCTTTGCTTGGCTTCATTTTCGGATTTGTACTCGGTGGGAACTGGTTAATTGTTGACATGTTAAAATTTAACATCACTATCTGTGATGTTGAGGGGCAAGGTAAAGAGGAGGACCATTTTGAACTCTTCATATCTACGCCATCTGCCAATATTGAATCCAGTCATGTCTTTTCTGATCTATAACCAATAAAACGCCAAAGTTATAACAAGTTTTAAAATCAAGGTAACAACACGTTTATCATCCATGCATCCTAGAAGTTATTCATACATATGCATGCTGCTAAAAGGGTACGGTGTTAACATAATGGATACCTCAGCATATCACAGTTTCAGTCAGATGATGTGATTGCCTGATTCTACTTGAAATATTTCAAGAGGAATTGAGGTGTGAATATTATTGTGTAAAATATATTTTACACTGTTATCATTGTGTGTCTTTTTCCCAAGCTTACTATCTTGAACCTAAAGAAGAGAAGCTTCTTGTCTTTGATTACATGAGTAGAGGAAGCCTAGCATCATTCCTTCACAGTAAGCACTAAGTAGTTCTTAGACTTCATTTGAGTTTGAGATTTTTTTTTTTTTTTTGTCTTAAAGCAATCACACATTGTTGATTATATGTTCCAGAGGATCATGATTAGAAGGCACGTTTCAACTTTGTATTATAATTTTGGACAATCCGATCTTGGTTCAATGGTCCAGATCATATGATTGTTATACTAGTGAACAAGATTAGAAGACAGATATTCCATCTTTGGATTATCGTTATGGACATTTATTAATGTTGGAACATGGACTATCCAATCTTGATACAAAGGTCTAGACTCCAGATCACATGATTGCGTGATTTCCTAACCGCAAGTAAACCAAAACTTACTTAGAAAACATTGCTTTTATCTCTTAAATTTATATGATAATACTGATGTGATATTCAAATTTTTAAACATATATTTGAAGCTTCTGTAACTGAAATCGGTTTCGAATGGCCCAACAAGGATGAAAATAGCAATTGGAATCGCCAATGGTCTCTCTTGCCTCCACAACCAAAAGAATATAGTGCATGGAATTCTCACCTCAAGCAACATACAATTGGTCTTTCAAGACTAATGACAACTTCAGCCAACACCAACATCATTGCTACAGCAGGAAGTCTTGGATACAATGCACCAGAGCTTTCAAAGACAAAGAAACCTACGACAAAAACCGATGTTTACAGCCTCGGTGTTATCCTTTTGGAACTCTTAACTGGTAAACCACCAGGAGAACCAACAAATGGCATGGACTTGCCTCAATATGTTGCATCCATTGTTAAAGAAGAATGGACAAATGAAGTGTTTGATTTGGAACTCATGAGGGATGGACCAACCATAGGAGATGAACTTCTTAAAATTAGCTTTGCATTGTGTTGA
Protein sequence

>MS.gene24983.t1

MQGEQEKGSSEHDFHNKFNDYNDHIDSYLSSSEPRSPLLGFIFGFVLGGNWLIVDMLKFNITICDVEGQAYYLEPKEEKLLVFDYMSRGSLASFLHTSVTEIGFEWPNKDENSNWNRQWSLLPPQPKEYSAWNSHLKQHTIGLSRLMTTSANTNIIATAGSLGYNAPELSKTKKPTTKTDVYSLGVILLELLTGKPPGEPTNGMDLPQYVASIVKEEWTNEVFDLELMRDGPTIGDELLKISFALC