AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24985

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24985.t1 MTR_4g014070 94.737 76 4 0 50 125 646 721 2.78e-35 128
MS.gene24985.t1 MTR_5g083480 72.619 84 23 0 42 125 633 716 3.57e-33 122
MS.gene24985.t1 MTR_5g083480 72.619 84 23 0 42 125 630 713 3.82e-33 122
MS.gene24985.t1 MTR_2g030380 43.678 87 43 2 52 132 915 1001 1.14e-12 64.3
MS.gene24985.t1 MTR_2g030380 43.678 87 43 2 52 132 933 1019 1.15e-12 64.3
MS.gene24985.t1 MTR_8g107470 43.038 79 37 2 50 125 520 593 1.24e-12 63.9
MS.gene24985.t1 MTR_3g094710 43.182 88 44 2 51 132 899 986 1.37e-12 63.9
MS.gene24985.t1 MTR_5g014700 39.796 98 53 4 39 132 828 923 1.51e-12 63.9
MS.gene24985.t1 MTR_6g470960 44.304 79 36 2 50 125 523 596 2.49e-12 63.2
MS.gene24985.t1 MTR_4g113100 43.038 79 37 2 50 125 492 565 3.31e-12 62.8
MS.gene24985.t1 MTR_4g097880 38.776 98 54 4 39 132 825 920 3.81e-12 62.8
MS.gene24985.t1 MTR_2g070020 45.070 71 38 1 50 119 955 1025 4.71e-12 62.4
MS.gene24985.t1 MTR_7g059225 39.773 88 46 2 50 132 527 612 6.00e-12 62.0
MS.gene24985.t1 MTR_5g087780 43.750 64 34 1 52 113 859 922 1.52e-11 60.8
MS.gene24985.t1 MTR_4g107620 34.444 90 54 2 42 126 449 538 2.35e-11 60.5
MS.gene24985.t1 MTR_5g078080 42.308 78 37 2 50 124 485 557 2.39e-11 60.5
MS.gene24985.t1 MTR_3g090665 43.750 80 38 2 50 126 201 276 2.80e-11 60.1
MS.gene24985.t1 MTR_2g010470 43.590 78 39 3 50 124 838 913 3.51e-11 59.7
MS.gene24985.t1 MTR_1g079520 43.590 78 42 1 50 125 914 991 4.50e-11 59.7
MS.gene24985.t1 MTR_3g062500 41.772 79 38 2 50 125 511 584 5.41e-11 59.3
MS.gene24985.t1 MTR_3g062500 41.772 79 38 2 50 125 501 574 5.61e-11 59.3
MS.gene24985.t1 MTR_5g075630 43.750 80 35 3 50 125 502 575 6.74e-11 58.9
MS.gene24985.t1 MTR_1g047670 41.026 78 45 1 50 126 957 1034 7.98e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24985.t1 AT3G51740 70.238 84 23 1 42 125 674 755 1.31e-32 121
MS.gene24985.t1 AT2G26730 44.872 78 35 2 51 125 495 567 3.57e-13 65.5
MS.gene24985.t1 AT1G17230 44.737 76 41 1 50 124 945 1020 3.73e-13 65.5
MS.gene24985.t1 AT1G17230 44.737 76 41 1 50 124 945 1020 4.07e-13 65.5
MS.gene24985.t1 AT3G49670 41.573 89 48 3 42 126 825 913 4.90e-13 65.1
MS.gene24985.t1 AT3G24660 40.230 87 48 2 50 132 519 605 9.34e-13 64.3
MS.gene24985.t1 AT4G20940 42.529 87 44 2 52 132 903 989 1.02e-12 64.3
MS.gene24985.t1 AT5G65700 40.449 89 49 3 42 126 829 917 2.69e-12 63.2
MS.gene24985.t1 AT5G65700 40.449 89 49 3 42 126 829 917 2.69e-12 63.2
MS.gene24985.t1 AT5G63930 41.026 78 45 1 50 126 951 1028 3.83e-12 62.8
MS.gene24985.t1 AT5G63930 41.026 78 45 1 50 126 887 964 4.12e-12 62.8
MS.gene24985.t1 AT3G17840 40.909 88 44 3 42 125 500 583 7.21e-12 62.0
MS.gene24985.t1 AT5G10020 42.353 85 43 2 54 132 870 954 8.90e-12 61.6
MS.gene24985.t1 AT3G08680 40.000 85 44 2 44 125 481 561 1.21e-11 61.2
MS.gene24985.t1 AT3G08680 40.000 85 44 2 44 125 481 561 1.21e-11 61.2
MS.gene24985.t1 AT5G41680 48.387 62 29 1 67 125 240 301 1.25e-11 60.8
MS.gene24985.t1 AT5G41680 48.387 62 29 1 67 125 214 275 1.32e-11 60.8
MS.gene24985.t1 AT1G08590 39.535 86 49 2 50 133 873 957 1.47e-11 60.8
MS.gene24985.t1 AT2G27060 37.143 105 55 4 34 132 867 966 2.15e-11 60.5
MS.gene24985.t1 AT2G27060 37.143 105 55 4 34 132 774 873 2.71e-11 60.1
MS.gene24985.t1 AT1G64210 39.759 83 39 3 50 125 450 528 2.81e-11 60.1
MS.gene24985.t1 AT1G68400 48.438 64 28 1 67 125 521 584 3.03e-11 60.1
MS.gene24985.t1 AT1G48480 41.463 82 41 2 46 124 511 588 3.26e-11 60.1
MS.gene24985.t1 AT2G33170 46.269 67 35 1 50 115 971 1037 3.86e-11 59.7
MS.gene24985.t1 AT2G33170 46.269 67 35 1 50 115 971 1037 3.86e-11 59.7
MS.gene24985.t1 AT5G07620 42.308 78 41 2 50 123 223 300 4.92e-11 59.3
MS.gene24985.t1 AT5G62710 36.709 79 47 1 51 126 458 536 7.19e-11 58.9
MS.gene24985.t1 AT5G62710 36.709 79 47 1 51 126 424 502 8.82e-11 58.5

Find 37 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCCTCGGTGTTATCCTTT+TGG 0.228699 4.1:-82221909 MS.gene24985:CDS
GGCCATTCGAAACCGATTTC+AGG 0.257792 4.1:+82222157 None:intergenic
TTCCATGCACTATATTCTTT+TGG 0.283746 4.1:+82222079 None:intergenic
GACAAATGAAGTGTTTGATT+TGG 0.323614 4.1:-82221802 MS.gene24985:CDS
ATGCCATTTGTTGGTTCTCC+TGG 0.362960 4.1:+82221866 None:intergenic
GAAGCTTCTGTACCTGAAAT+CGG 0.391294 4.1:-82222169 MS.gene24985:intron
GGCAAGTCCATGCCATTTGT+TGG 0.425256 4.1:+82221857 None:intergenic
ACAAGGATGAAAATAGCAAT+TGG 0.434911 4.1:-82222132 MS.gene24985:CDS
CACCTCAAGCAACATACAAT+TGG 0.458004 4.1:-82222052 MS.gene24985:CDS
ACAATGGATGCAACATATTG+AGG 0.461356 4.1:+82221836 None:intergenic
TAAACGTCTGTCTTTGTGGT+AGG 0.464968 4.1:+82221932 None:intergenic
ATTGCTACAGCAGGAAGTCT+TGG 0.477792 4.1:-82221989 MS.gene24985:CDS
GGTGTAAACGTCTGTCTTTG+TGG 0.505112 4.1:+82221928 None:intergenic
TCCTGCTGTAGCAATGATGT+TGG 0.533607 4.1:+82221997 None:intergenic
CCACCAGGAGAACCAACAAA+TGG 0.539629 4.1:-82221869 MS.gene24985:CDS
ACCAACATCATTGCTACAGC+AGG 0.542435 4.1:-82221998 MS.gene24985:CDS
GTTACAATGTTGAGGGGCAA+AGG 0.546455 4.1:-82223891 None:intergenic
CCATTTGTTGGTTCTCCTGG+TGG 0.548920 4.1:+82221869 None:intergenic
TGTAGCAATGATGTTGGTGT+TGG 0.550967 4.1:+82222003 None:intergenic
AACCAAAAGAATATAGTGCA+TGG 0.563084 4.1:-82222081 MS.gene24985:CDS
CTCTTAACTGGTAAACCACC+AGG 0.563734 4.1:-82221884 MS.gene24985:CDS
TACCAGTTAAGAGCTCCAAA+AGG 0.568751 4.1:+82221894 None:intergenic
ACGGGGTGTTACAATGTTGA+GGG 0.571421 4.1:-82223898 None:intergenic
AAGACAGACGTTTACACCCT+CGG 0.572563 4.1:-82221923 MS.gene24985:CDS
AACGGGGTGTTACAATGTTG+AGG 0.572769 4.1:-82223899 None:intergenic
TGTTTGATTTGGAACTCATG+AGG 0.576156 4.1:-82221791 None:intergenic
AGGAGAACCAACAAATGGCA+TGG 0.605960 4.1:-82221864 MS.gene24985:CDS
TACCTGAAATCGGTTTCGAA+TGG 0.610998 4.1:-82222159 MS.gene24985:CDS
ATAGCAATTGGAATCGCCAA+TGG 0.612433 4.1:-82222120 MS.gene24985:CDS
GTGGAGGCAAGAGAGACCAT+TGG 0.612548 4.1:+82222104 None:intergenic
CGGTTTCGAATGGCCCAACA+AGG 0.612620 4.1:-82222149 MS.gene24985:CDS
GTTTGATTTGGAACTCATGA+GGG 0.628775 4.1:-82221790 None:intergenic
GACCAATTGTATGTTGCTTG+AGG 0.629446 4.1:+82222050 None:intergenic
AAGTCTTGGATACAATGCAC+CGG 0.639160 4.1:-82221975 MS.gene24985:CDS
CTCCAAAAGGATAACACCGA+GGG 0.642486 4.1:+82221907 None:intergenic
CGGGGTGTTACAATGTTGAG+GGG 0.688873 4.1:-82223897 None:intergenic
GCTCCAAAAGGATAACACCG+AGG 0.748955 4.1:+82221906 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTATTTAAAAAGAAATTAA+GGG + chr4.1:82222586-82222605 None:intergenic 10.0%
!!! AAAAATTTCTAAAAGAAAAA+AGG + chr4.1:82222659-82222678 None:intergenic 10.0%
!! GTCTATTTAAAAAGAAATTA+AGG + chr4.1:82222587-82222606 None:intergenic 15.0%
!!! ATGTTATTTTAGTAAACTTT+AGG - chr4.1:82223271-82223290 MS.gene24985:intron 15.0%
!!! ATTTTTACTCGTATTTAATT+TGG - chr4.1:82223006-82223025 MS.gene24985:intron 15.0%
!!! TATAACAAGTTTTAAAATCA+AGG - chr4.1:82221910-82221929 MS.gene24985:CDS 15.0%
!!! TCAACTTTGTATTATAATTT+TGG - chr4.1:82222385-82222404 MS.gene24985:intron 15.0%
!!! TGTTATTTTAGTAAACTTTA+GGG - chr4.1:82223272-82223291 MS.gene24985:intron 15.0%
!!! TTTAAAACTTGTTATAACTT+TGG + chr4.1:82221906-82221925 None:intergenic 15.0%
!! AACACTTAACAGTAAATTAA+CGG + chr4.1:82222634-82222653 None:intergenic 20.0%
!! AATAAATGTCCATAATGATA+AGG + chr4.1:82223316-82223335 None:intergenic 20.0%
!! ATAAATGTCCATAATGATAA+GGG + chr4.1:82223315-82223334 None:intergenic 20.0%
!! ATTTAAAAAGAAATTAAGGG+AGG + chr4.1:82222583-82222602 None:intergenic 20.0%
!! TAAAGTTAATGGAAAGTTAA+TGG - chr4.1:82223164-82223183 MS.gene24985:intron 20.0%
!! TCCTCAAAAAATAAAGTTAA+TGG - chr4.1:82223153-82223172 MS.gene24985:intron 20.0%
!! TTATGGACATTTATTAATGT+TGG - chr4.1:82223321-82223340 MS.gene24985:intron 20.0%
!!! AATGTTTTCTAAGTAAGTTT+CGG + chr4.1:82223425-82223444 None:intergenic 20.0%
!!! GTGTAAGTTTTAAAACTAAA+GGG + chr4.1:82222557-82222576 None:intergenic 20.0%
!!! GTTATTTTAGTAAACTTTAG+GGG - chr4.1:82223273-82223292 MS.gene24985:intron 20.0%
!!! TCCATTAACTTTATTTTTTG+AGG + chr4.1:82223157-82223176 None:intergenic 20.0%
!!! TGTAAGTTTTAAAACTAAAG+GGG + chr4.1:82222556-82222575 None:intergenic 20.0%
! AAATATTTCAAGAGGAATTG+AGG - chr4.1:82222072-82222091 MS.gene24985:CDS 25.0%
! AGTATAACAATCATATGATC+TGG + chr4.1:82222435-82222454 None:intergenic 25.0%
! CATATGTATGAATAACTTCT+AGG + chr4.1:82221962-82221981 None:intergenic 25.0%
! TAGATATGAAGAGTTCAAAA+TGG + chr4.1:82221825-82221844 None:intergenic 25.0%
! TCTACTTGAAATATTTCAAG+AGG - chr4.1:82222064-82222083 MS.gene24985:CDS 25.0%
! TGTATTTGATGAGAACTTAA+GGG - chr4.1:82223212-82223231 MS.gene24985:intron 25.0%
!! ATTAACGGTGTTAATAGAAA+AGG + chr4.1:82222619-82222638 None:intergenic 25.0%
!! GAAATATTTCAAGTAGAATC+AGG + chr4.1:82222061-82222080 None:intergenic 25.0%
!! TTAACGGTGTTAATAGAAAA+GGG + chr4.1:82222618-82222637 None:intergenic 25.0%
!! TTAATGGTGTTAATGGAAAA+GGG - chr4.1:82223180-82223199 MS.gene24985:intron 25.0%
!! TTATAACTTTGGCGTTTTAT+TGG + chr4.1:82221895-82221914 None:intergenic 25.0%
!! TTGTCCATTCTTTTTAACAA+TGG + chr4.1:82223861-82223880 None:intergenic 25.0%
!! TTTAGTTGATTCATCATCAA+TGG - chr4.1:82223093-82223112 MS.gene24985:intron 25.0%
!!! ACAATGAAGATTTTTTTTCC+TGG - chr4.1:82223124-82223143 MS.gene24985:intron 25.0%
!!! AGTAAAAATCTGTTTTAGCA+TGG + chr4.1:82222995-82223014 None:intergenic 25.0%
!!! AGTGTATGTTTTAAAACTAG+AGG - chr4.1:82223240-82223259 MS.gene24985:intron 25.0%
!!! AGTTTTATCAATGATGGATT+TGG + chr4.1:82222781-82222800 None:intergenic 25.0%
!!! ATTTTTTGAGGAAATTTTCC+AGG + chr4.1:82223145-82223164 None:intergenic 25.0%
!!! GCTTTTAGTTTTATCAATGA+TGG + chr4.1:82222787-82222806 None:intergenic 25.0%
!!! GGTGTAAGTTTTAAAACTAA+AGG + chr4.1:82222558-82222577 None:intergenic 25.0%
!!! GTAAAAATCTGTTTTAGCAT+GGG + chr4.1:82222994-82223013 None:intergenic 25.0%
!!! GTAAGTTTTAAAACTAAAGG+GGG + chr4.1:82222555-82222574 None:intergenic 25.0%
!!! GTCATTTTAGTAAACCTTAA+GGG + chr4.1:82222524-82222543 None:intergenic 25.0%
!!! GTGTATGTTTTAAAACTAGA+GGG - chr4.1:82223241-82223260 MS.gene24985:intron 25.0%
!!! TAAGTTTTAAAACTAAAGGG+GGG + chr4.1:82222554-82222573 None:intergenic 25.0%
!!! TGTATGTTTTAAAACTAGAG+GGG - chr4.1:82223242-82223261 MS.gene24985:intron 25.0%
!!! TGTCATTTTAGTAAACCTTA+AGG + chr4.1:82222525-82222544 None:intergenic 25.0%
AAAAAGAAATTAAGGGAGGT+TGG + chr4.1:82222579-82222598 None:intergenic 30.0%
AACCAAAAGAATATAGTGCA+TGG - chr4.1:82223597-82223616 MS.gene24985:intron 30.0%
ACAAGGATGAAAATAGCAAT+TGG - chr4.1:82223546-82223565 MS.gene24985:intron 30.0%
ATGGTGATTCTTGAATTACT+AGG - chr4.1:82222691-82222710 MS.gene24985:intron 30.0%
ATGGTTATCAACTGTGTTTA+CGG + chr4.1:82222741-82222760 None:intergenic 30.0%
CATTTATTAATGTTGGAACC+TGG - chr4.1:82223328-82223347 MS.gene24985:intron 30.0%
GCATCCATTGTTAAAAAGAA+TGG - chr4.1:82223854-82223873 MS.gene24985:intron 30.0%
GCTACAAATAATGTTGATAC+CGG + chr4.1:82222833-82222852 None:intergenic 30.0%
GGTTGATTACAACAATGAAA+TGG + chr4.1:82222760-82222779 None:intergenic 30.0%
GTGTATTTGATGAGAACTTA+AGG - chr4.1:82223211-82223230 MS.gene24985:intron 30.0%
TACTAGGGAGATGAAAAAAA+TGG - chr4.1:82222707-82222726 MS.gene24985:intron 30.0%
TGGTGATTCTTGAATTACTA+GGG - chr4.1:82222692-82222711 MS.gene24985:intron 30.0%
! GACAAATGAAGTGTTTGATT+TGG - chr4.1:82223876-82223895 MS.gene24985:intron 30.0%
! GTAATTTGCCCTTATCATTA+TGG - chr4.1:82223304-82223323 MS.gene24985:intron 30.0%
! GTTAATGGTGTTAATGGAAA+AGG - chr4.1:82223179-82223198 MS.gene24985:intron 30.0%
! TGGAAAGTTAATGGTGTTAA+TGG - chr4.1:82223173-82223192 MS.gene24985:intron 30.0%
! TTCCATGCACTATATTCTTT+TGG + chr4.1:82223602-82223621 None:intergenic 30.0%
! TTGTTGATTATATGTTCCAG+AGG - chr4.1:82222339-82222358 MS.gene24985:intron 30.0%
!! ATTTTAGTAAACCTTAAGGG+AGG + chr4.1:82222521-82222540 None:intergenic 30.0%
!! ATTTTAGTAAACTTTAGGGG+AGG - chr4.1:82223276-82223295 MS.gene24985:intron 30.0%
!! TGCATAACTTTGATTTGCAA+TGG + chr4.1:82222941-82222960 None:intergenic 30.0%
!!! ATTGCTATTTTCATCCTTGT+TGG + chr4.1:82223546-82223565 None:intergenic 30.0%
!!! GTATGTTTTAAAACTAGAGG+GGG - chr4.1:82223243-82223262 MS.gene24985:intron 30.0%
!!! TTGCTATTTTCATCCTTGTT+GGG + chr4.1:82223545-82223564 None:intergenic 30.0%
!!! TTTTTTCTCTTTGAAAGCTC+CGG + chr4.1:82223725-82223744 None:intergenic 30.0%
ACAAGAAGCTTCTCTTCTTT+AGG + chr4.1:82222173-82222192 None:intergenic 35.0%
ACAATGGATGCAACATATTG+AGG + chr4.1:82223845-82223864 None:intergenic 35.0%
ACATATGCATGCTGCTAAAA+GGG - chr4.1:82221975-82221994 MS.gene24985:CDS 35.0%
ACTATCCGATCTTGATTCAA+AGG - chr4.1:82223351-82223370 MS.gene24985:intron 35.0%
AGGTTCAAGATAGTAAGCTT+GGG + chr4.1:82222153-82222172 None:intergenic 35.0%
AGTAAGTTTCGGTTTACTTG+CGG + chr4.1:82223414-82223433 None:intergenic 35.0%
ATTTGATGAGAACTTAAGGG+AGG - chr4.1:82223215-82223234 MS.gene24985:intron 35.0%
CAGATGTTGAAACATCAGTA+AGG - chr4.1:82222883-82222902 MS.gene24985:intron 35.0%
CCATCTCTGGATTATCATTA+GGG - chr4.1:82222482-82222501 MS.gene24985:intron 35.0%
CTACTTAGTGTTTACTGTGA+AGG + chr4.1:82222232-82222251 None:intergenic 35.0%
CTAGACCTTTGAATCAAGAT+CGG + chr4.1:82223359-82223378 None:intergenic 35.0%
GAAACATCAGTAAGGAAGTT+AGG - chr4.1:82222891-82222910 MS.gene24985:intron 35.0%
GACATGACTGGATTCAATAT+TGG + chr4.1:82221857-82221876 None:intergenic 35.0%
GTCTTTGATTACATGAGTAG+AGG - chr4.1:82222191-82222210 MS.gene24985:intron 35.0%
TACATATGCATGCTGCTAAA+AGG - chr4.1:82221974-82221993 MS.gene24985:CDS 35.0%
TAGATCAGAAAAGACATGAC+TGG + chr4.1:82221869-82221888 None:intergenic 35.0%
TAGGTTCAAGATAGTAAGCT+TGG + chr4.1:82222154-82222173 None:intergenic 35.0%
TCCATCTCTGGATTATCATT+AGG - chr4.1:82222481-82222500 MS.gene24985:intron 35.0%
TTGCAATGGAGAAATTGTGT+CGG + chr4.1:82222927-82222946 None:intergenic 35.0%
! AATTTTGGACAATCCGATCT+TGG - chr4.1:82222400-82222419 MS.gene24985:intron 35.0%
!! CTATATTCTTTTGGTTGTGG+AGG + chr4.1:82223593-82223612 None:intergenic 35.0%
!! GCACTATATTCTTTTGGTTG+TGG + chr4.1:82223596-82223615 None:intergenic 35.0%
!! TGAATAACTTCTAGGATGCA+TGG + chr4.1:82221954-82221973 None:intergenic 35.0%
AAAAAAATCCTCCCCAGTGA+AGG + chr4.1:82223040-82223059 None:intergenic 40.0%
AAGTCTTGGATACAATGCAC+CGG - chr4.1:82223703-82223722 MS.gene24985:intron 40.0%
AATCACGCAATCATGTGATC+TGG + chr4.1:82223385-82223404 None:intergenic 40.0%
ACAATCCGATCTTGGTTCAA+TGG - chr4.1:82222408-82222427 MS.gene24985:intron 40.0%
ACTGAAACTGTGATATGCTG+AGG + chr4.1:82222023-82222042 None:intergenic 40.0%
ATAGCAATTGGAATCGCCAA+TGG - chr4.1:82223558-82223577 MS.gene24985:intron 40.0%
CACCTCAAGCAACATACAAT+TGG - chr4.1:82223626-82223645 MS.gene24985:intron 40.0%
CCCTAATGATAATCCAGAGA+TGG + chr4.1:82222485-82222504 None:intergenic 40.0%
CTGGATTCAATATTGGCAGA+TGG + chr4.1:82221850-82221869 None:intergenic 40.0%
GAAGACAGATATTCCATCTC+TGG - chr4.1:82222469-82222488 MS.gene24985:intron 40.0%
GAAGCTTCTGTACCTGAAAT+CGG - chr4.1:82223509-82223528 MS.gene24985:intron 40.0%
TAAACGTCTGTCTTTGTGGT+AGG + chr4.1:82223749-82223768 None:intergenic 40.0%
TACCAGTTAAGAGCTCCAAA+AGG + chr4.1:82223787-82223806 None:intergenic 40.0%
TACTGTGAAGGAATGATGCT+AGG + chr4.1:82222220-82222239 None:intergenic 40.0%
TAGTAAACCTTAAGGGAGGT+GGG + chr4.1:82222517-82222536 None:intergenic 40.0%
TCCAGAGGATCATGATTAGA+AGG - chr4.1:82222354-82222373 MS.gene24985:intron 40.0%
TGTAGCAATGATGTTGGTGT+TGG + chr4.1:82223678-82223697 None:intergenic 40.0%
TTAGTAAACCTTAAGGGAGG+TGG + chr4.1:82222518-82222537 None:intergenic 40.0%
TTAGTGCAATCAAACACAGC+CGG - chr4.1:82222811-82222830 MS.gene24985:intron 40.0%
TTGGTTCAACAACCTTCACT+GGG - chr4.1:82223025-82223044 MS.gene24985:intron 40.0%
! ACGGTGTTAATAGAAAAGGG+AGG + chr4.1:82222615-82222634 None:intergenic 40.0%
! AGGGTACGGTGTTAACATAA+TGG - chr4.1:82221994-82222013 MS.gene24985:CDS 40.0%
! ATGGTGTTAATGGAAAAGGG+AGG - chr4.1:82223183-82223202 MS.gene24985:intron 40.0%
! GACCAATTGTATGTTGCTTG+AGG + chr4.1:82223631-82223650 None:intergenic 40.0%
! TACCTGAAATCGGTTTCGAA+TGG - chr4.1:82223519-82223538 MS.gene24985:intron 40.0%
! TTAAAACTAAAGGGGGGTGT+TGG + chr4.1:82222548-82222567 None:intergenic 40.0%
! TTTGGTTCAACAACCTTCAC+TGG - chr4.1:82223024-82223043 MS.gene24985:intron 40.0%
!!! ATCCTTTTGGAGCTCTTAAC+TGG - chr4.1:82223782-82223801 MS.gene24985:intron 40.0%
AAGACAGACGTTTACACCCT+CGG - chr4.1:82223755-82223774 MS.gene24985:intron 45.0%
AATTACACCCACCTCCCTTA+AGG - chr4.1:82222507-82222526 MS.gene24985:intron 45.0%
ACCAACATCATTGCTACAGC+AGG - chr4.1:82223680-82223699 MS.gene24985:intron 45.0%
AGGAGAACCAACAAATGGCA+TGG - chr4.1:82223814-82223833 MS.gene24985:intron 45.0%
ATTGCTACAGCAGGAAGTCT+TGG - chr4.1:82223689-82223708 MS.gene24985:intron 45.0%
CTCCAAAAGGATAACACCGA+GGG + chr4.1:82223774-82223793 None:intergenic 45.0%
CTCTTAACTGGTAAACCACC+AGG - chr4.1:82223794-82223813 MS.gene24985:intron 45.0%
CTGGACCATTGAACCAAGAT+CGG + chr4.1:82222416-82222435 None:intergenic 45.0%
GAATCAAGATCGGATAGTCC+AGG + chr4.1:82223349-82223368 None:intergenic 45.0%
GCCTTCTAATCATGATCCTC+TGG + chr4.1:82222358-82222377 None:intergenic 45.0%
GGTGTAAACGTCTGTCTTTG+TGG + chr4.1:82223753-82223772 None:intergenic 45.0%
GTTTCGGTTTACTTGCGGTT+AGG + chr4.1:82223409-82223428 None:intergenic 45.0%
TCCTGCTGTAGCAATGATGT+TGG + chr4.1:82223684-82223703 None:intergenic 45.0%
TGCATGCTGCTAAAAGGGTA+CGG - chr4.1:82221980-82221999 MS.gene24985:CDS 45.0%
TGGTTCAACAACCTTCACTG+GGG - chr4.1:82223026-82223045 MS.gene24985:intron 45.0%
! GGAGAAATTGTGTCGGTGTT+TGG + chr4.1:82222920-82222939 None:intergenic 45.0%
!! ATGCCATTTGTTGGTTCTCC+TGG + chr4.1:82223815-82223834 None:intergenic 45.0%
!!! AATTTTTTATCAATATTTAA+TGG - chr4.1:82222672-82222691 MS.gene24985:intron 5.0%
CCACCAGGAGAACCAACAAA+TGG - chr4.1:82223809-82223828 MS.gene24985:intron 50.0%
GCTCCAAAAGGATAACACCG+AGG + chr4.1:82223775-82223794 None:intergenic 50.0%
GTTGAGGGGCAAAGGTAAAG+AGG - chr4.1:82221795-82221814 MS.gene24985:CDS 50.0%
TTCAACAACCTTCACTGGGG+AGG - chr4.1:82223029-82223048 MS.gene24985:intron 50.0%
! GGCAAGTCCATGCCATTTGT+TGG + chr4.1:82223824-82223843 None:intergenic 50.0%
! GGCCATTCGAAACCGATTTC+AGG + chr4.1:82223524-82223543 None:intergenic 50.0%
!! CCATTTGTTGGTTCTCCTGG+TGG + chr4.1:82223812-82223831 None:intergenic 50.0%
!!! CACCCTCGGTGTTATCCTTT+TGG - chr4.1:82223769-82223788 MS.gene24985:intron 50.0%
CGGTTTCGAATGGCCCAACA+AGG - chr4.1:82223529-82223548 MS.gene24985:intron 55.0%
GAGGGGCAAAGGTAAAGAGG+AGG - chr4.1:82221798-82221817 MS.gene24985:CDS 55.0%
GTGGAGGCAAGAGAGACCAT+TGG + chr4.1:82223577-82223596 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 82221793 82223907 82221793 ID=MS.gene24985
chr4.1 mRNA 82221793 82223907 82221793 ID=MS.gene24985.t1;Parent=MS.gene24985
chr4.1 exon 82223892 82223907 82223892 ID=MS.gene24985.t1.exon1;Parent=MS.gene24985.t1
chr4.1 CDS 82223892 82223907 82223892 ID=cds.MS.gene24985.t1;Parent=MS.gene24985.t1
chr4.1 exon 82221793 82222187 82221793 ID=MS.gene24985.t1.exon2;Parent=MS.gene24985.t1
chr4.1 CDS 82221793 82222187 82221793 ID=cds.MS.gene24985.t1;Parent=MS.gene24985.t1
Gene Sequence

>MS.gene24985

ATGTTGAGGGGCAAAGGTAAAGAGGAGGACCATTTTGAACTCTTCATATCTACGCCATCTGCCAATATTGAATCCAGTCATGTCTTTTCTGATCTATAACCAATAAAACGCCAAAGTTATAACAAGTTTTAAAATCAAGGTAACAACACGTTTATCATCCATGCATCCTAGAAGTTATTCATACATATGCATGCTGCTAAAAGGGTACGGTGTTAACATAATGGATACCTCAGCATATCACAGTTTCAGTCAGATGATGTGATTGCCTGATTCTACTTGAAATATTTCAAGAGGAATTGAGGTGTGAATATTATTGTGTAAAATATATTTTACACTGTTATCATTGTGTGTCTTTTTCCCAAGCTTACTATCTTGAACCTAAAGAAGAGAAGCTTCTTGTCTTTGATTACATGAGTAGAGGAAGCCTAGCATCATTCCTTCACAGTAAACACTAAGTAGTTCTTAGACTTCATTTGAGTTTGAGTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTATTTTGTCTTAAAGCAATCACACATTGTTGATTATATGTTCCAGAGGATCATGATTAGAAGGCACATGTTTCAACTTTGTATTATAATTTTGGACAATCCGATCTTGGTTCAATGGTCCAGATCATATGATTGTTATACTAGTGAACAAGATTAGAAGACAGATATTCCATCTCTGGATTATCATTAGGGAAAATTACACCCACCTCCCTTAAGGTTTACTAAAATGACACCAACACCCCCCTTTAGTTTTAAAACTTACACCAACCTCCCTTAATTTCTTTTTAAATAGACAAACACCTCCCTTTTCTATTAACACCGTTAATTTACTGTTAAGTGTTAACCTTTTTTCTTTTAGAAATTTTTTATCAATATTTAATGGTGATTCTTGAATTACTAGGGAGATGAAAAAAATGGCAAAAGCACCGTAAACACAGTTGATAACCATTTCATTGTTGTAATCAACCAAATCCATCATTGATAAAACTAAAAGCACTGTTAGTGCAATCAAACACAGCCGGTATCAACATTATTTGTAGCAATTTAATATAGAAAGTAAGCGTACAGTAGCAGATGTTGAAACATCAGTAAGGAAGTTAGGAAGCCAAACACCGACACAATTTCTCCATTGCAAATCAAAGTTATGCATAAAAGTAACTCAACACAATTCAACAAATTCCCATGCTAAAACAGATTTTTACTCGTATTTAATTTGGTTCAACAACCTTCACTGGGGAGGATTTTTTTTTTTTTTTTTTGATCTAATTACTTGTATTTAGATTTAGTTGATTCATCATCAATGGTAAGTTTAACAATGAAGATTTTTTTTCCTGGAAAATTTCCTCAAAAAATAAAGTTAATGGAAAGTTAATGGTGTTAATGGAAAAGGGAGGTGTTTGTGTATTTGATGAGAACTTAAGGGAGGTTAGTGTATGTTTTAAAACTAGAGGGGGTGTCAATGTTATTTTAGTAAACTTTAGGGGAGGTGAGTGTAATTTGCCCTTATCATTATGGACATTTATTAATGTTGGAACCTGGACTATCCGATCTTGATTCAAAGGTCTAGACTCCAGATCACATGATTGCGTGATTTCCTAACCGCAAGTAAACCGAAACTTACTTAGAAAACATTGCTTTTATCTCTTAAATTTATATGATAATACTGATATGATATTCAAATTTTTAAACATATATTTGAAGCTTCTGTACCTGAAATCGGTTTCGAATGGCCCAACAAGGATGAAAATAGCAATTGGAATCGCCAATGGTCTCTCTTGCCTCCACAACCAAAAGAATATAGTGCATGGAATTCTCACCTCAAGCAACATACAATTGGTCTTTCAAGACTAATGACAACTTCAGCCAACACCAACATCATTGCTACAGCAGGAAGTCTTGGATACAATGCACCGGAGCTTTCAAAGAGAAAAAAACCTACCACAAAGACAGACGTTTACACCCTCGGTGTTATCCTTTTGGAGCTCTTAACTGGTAAACCACCAGGAGAACCAACAAATGGCATGGACTTGCCTCAATATGTTGCATCCATTGTTAAAAAGAATGGACAAATGAAGTGTTTGATTTGGAACTCATGA
Protein sequence

>MS.gene24985.t1

MLRGKASVPEIGFEWPNKDENSNWNRQWSLLPPQPKEYSAWNSHLKQHTIGLSRLMTTSANTNIIATAGSLGYNAPELSKRKKPTTKTDVYTLGVILLELLTGKPPGEPTNGMDLPQYVASIVKKNGQMKCLIWNS