AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25140.t1 MTR_1g008290 66.154 130 41 1 94 223 8 134 1.59e-62 192
MS.gene25140.t1 MTR_5g032260 78.313 83 18 0 92 174 323 405 1.34e-41 146
MS.gene25140.t1 MTR_0402s0040 29.570 186 122 3 41 219 26 209 8.22e-22 93.2
MS.gene25140.t1 MTR_1g070355 28.889 225 119 7 14 204 27 244 1.07e-20 90.1
MS.gene25140.t1 MTR_2g098880 37.736 106 60 1 40 139 60 165 6.12e-20 85.5
MS.gene25140.t1 MTR_2g098880 37.736 106 60 1 40 139 72 177 7.59e-20 85.5
MS.gene25140.t1 MTR_5g076070 29.524 210 119 9 25 217 33 230 3.30e-19 84.0
MS.gene25140.t1 MTR_4g067290 34.188 117 72 2 28 140 43 158 5.13e-16 74.7
MS.gene25140.t1 MTR_4g067290 34.188 117 72 2 28 140 43 158 5.56e-16 74.3
MS.gene25140.t1 MTR_4g067290 35.714 112 67 2 33 140 66 176 5.70e-16 74.7
MS.gene25140.t1 MTR_4g067290 35.714 112 67 2 33 140 68 178 6.10e-16 74.7
MS.gene25140.t1 MTR_4g067290 35.714 112 67 2 33 140 68 178 6.27e-16 74.7
MS.gene25140.t1 MTR_4g067290 35.714 112 67 2 33 140 108 218 1.02e-15 74.7
MS.gene25140.t1 MTR_3g006370 34.862 109 66 1 36 139 45 153 2.31e-15 72.8
MS.gene25140.t1 MTR_7g446520 28.111 217 123 7 13 216 56 252 8.43e-15 72.4
MS.gene25140.t1 MTR_2g098920 33.566 143 81 3 11 140 41 182 1.96e-14 70.9
MS.gene25140.t1 MTR_5g015480 33.600 125 77 3 41 159 56 180 1.14e-12 65.9
MS.gene25140.t1 MTR_7g446060 35.294 85 55 0 122 206 6 90 3.54e-12 62.0
MS.gene25140.t1 MTR_1g019850 27.673 159 102 4 27 175 48 203 1.04e-11 62.8
MS.gene25140.t1 MTR_6g051425 20.707 198 138 4 33 216 9 201 2.79e-11 62.4
MS.gene25140.t1 MTR_0856s0010 29.688 128 87 2 36 161 29 155 3.27e-11 60.1
MS.gene25140.t1 MTR_4g035820 27.273 231 146 8 2 215 33 258 3.52e-11 62.4
MS.gene25140.t1 MTR_7g446480 34.737 95 61 1 123 216 151 245 7.52e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25140.t1 AT4G15090 49.206 189 91 2 40 223 50 238 6.72e-53 182
MS.gene25140.t1 AT4G15090 49.206 189 91 2 40 223 50 238 6.72e-53 182
MS.gene25140.t1 AT4G15090 49.206 189 91 2 40 223 50 238 6.72e-53 182
MS.gene25140.t1 AT4G15090 49.206 189 91 2 40 223 50 238 6.72e-53 182
MS.gene25140.t1 AT4G15090 49.206 189 91 2 40 223 50 238 6.72e-53 182
MS.gene25140.t1 AT1G76320 44.751 181 92 3 45 223 1 175 5.34e-43 154
MS.gene25140.t1 AT1G76320 44.751 181 92 3 45 223 1 175 5.34e-43 154
MS.gene25140.t1 AT3G22170 41.000 200 79 3 40 222 69 246 5.42e-43 154
MS.gene25140.t1 AT3G22170 41.000 200 79 3 40 222 69 246 5.42e-43 154
MS.gene25140.t1 AT1G76320 44.751 181 92 3 45 223 1 175 5.45e-43 154
MS.gene25140.t1 AT1G76320 44.751 181 92 3 45 223 1 175 5.87e-43 153
MS.gene25140.t1 AT2G32250 53.571 112 49 2 44 154 41 150 6.39e-31 120
MS.gene25140.t1 AT2G32250 53.571 112 49 2 44 154 41 150 6.53e-31 120
MS.gene25140.t1 AT2G32250 53.571 112 49 2 44 154 41 150 6.53e-31 120
MS.gene25140.t1 AT2G32250 53.571 112 49 2 44 154 41 150 6.53e-31 120
MS.gene25140.t1 AT2G32250 53.571 112 49 2 44 154 41 150 6.78e-31 119
MS.gene25140.t1 AT2G32250 53.571 112 49 2 44 154 41 150 6.78e-31 119
MS.gene25140.t1 AT2G32250 53.153 111 49 2 45 154 1 109 5.91e-30 117
MS.gene25140.t1 AT2G32250 53.153 111 49 2 45 154 1 109 5.92e-30 117
MS.gene25140.t1 AT4G38180 36.842 190 111 3 40 221 72 260 2.05e-29 115
MS.gene25140.t1 AT4G19990 34.483 174 88 3 1 157 4 168 3.00e-22 95.1
MS.gene25140.t1 AT4G19990 40.000 135 64 1 40 157 20 154 4.25e-22 94.4
MS.gene25140.t1 AT4G19990 40.000 135 64 1 40 157 20 154 4.25e-22 94.4
MS.gene25140.t1 AT4G19990 42.735 117 50 1 44 143 24 140 5.14e-22 94.4
MS.gene25140.t1 AT4G19990 42.735 117 50 1 44 143 24 140 5.14e-22 94.4
MS.gene25140.t1 AT4G19990 40.000 135 64 1 40 157 20 154 5.20e-22 94.4
MS.gene25140.t1 AT3G07500 38.298 141 70 5 12 140 1 136 1.18e-17 78.6
MS.gene25140.t1 AT3G59470 33.088 136 85 3 8 140 40 172 4.92e-17 77.8
MS.gene25140.t1 AT3G59470 33.088 136 85 3 8 140 40 172 4.92e-17 77.8
MS.gene25140.t1 AT3G59470 32.847 137 86 3 8 141 52 185 5.54e-17 77.8
MS.gene25140.t1 AT1G80010 30.526 190 123 3 41 222 95 283 5.40e-16 76.6
MS.gene25140.t1 AT1G10240 26.106 226 150 7 5 217 14 235 9.03e-16 76.3
MS.gene25140.t1 AT1G10240 26.106 226 150 7 5 217 14 235 9.03e-16 76.3
MS.gene25140.t1 AT5G18960 36.667 120 67 1 22 141 192 302 2.06e-15 75.1
MS.gene25140.t1 AT5G18960 34.454 119 69 2 20 138 22 131 7.06e-15 73.6
MS.gene25140.t1 AT5G18960 36.667 120 67 1 22 141 192 302 2.06e-15 75.1
MS.gene25140.t1 AT5G18960 34.454 119 69 2 20 138 22 131 7.06e-15 73.6
MS.gene25140.t1 AT2G43280 33.654 104 64 1 40 138 21 124 1.25e-14 70.5
MS.gene25140.t1 AT1G52520 30.822 146 91 2 2 138 38 182 4.10e-14 71.2
MS.gene25140.t1 AT4G12850 35.000 100 64 1 40 138 10 109 8.35e-14 66.6
MS.gene25140.t1 AT4G12850 35.000 100 64 1 40 138 10 109 1.70e-13 65.9
MS.gene25140.t1 AT4G12850 35.000 100 64 1 40 138 10 109 1.87e-13 66.6
MS.gene25140.t1 AT3G06250 37.255 102 55 1 40 141 189 281 3.19e-13 68.6
MS.gene25140.t1 AT3G06250 34.286 105 60 2 34 138 21 116 5.02e-13 68.2
MS.gene25140.t1 AT3G06250 37.255 102 55 1 40 141 189 281 3.19e-13 68.6
MS.gene25140.t1 AT3G06250 34.286 105 60 2 34 138 21 116 5.02e-13 68.2
MS.gene25140.t1 AT3G06250 37.255 102 55 1 40 141 189 281 3.19e-13 68.6
MS.gene25140.t1 AT3G06250 34.286 105 60 2 34 138 21 116 5.02e-13 68.2
MS.gene25140.t1 AT5G28530 25.543 184 119 9 41 211 55 233 6.22e-11 62.0
MS.gene25140.t1 AT5G28530 25.543 184 119 9 41 211 55 233 6.44e-11 62.0
MS.gene25140.t1 AT5G28530 25.543 184 119 9 41 211 55 233 6.44e-11 62.0
MS.gene25140.t1 AT5G28530 25.543 184 119 9 41 211 55 233 6.83e-11 61.6
MS.gene25140.t1 AT5G28530 25.543 184 119 9 41 211 55 233 6.84e-11 61.6

Find 42 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAATTTGTGATGGAAAA+TGG 0.198140 8.4:+72989854 MS.gene25140:CDS
AACGCCGACCTTTATCTAAA+TGG 0.237387 8.4:-72990089 None:intergenic
TATGCTAAGTCAGTTGGATT+TGG 0.280799 8.4:+72989679 MS.gene25140:CDS
TTCATGTACCGTTGATTTAG+AGG 0.293148 8.4:+72989582 MS.gene25140:CDS
GAGCTTTATTGATTCCTCTA+TGG 0.299611 8.4:-72989945 None:intergenic
TCAAATTCCATTCCAATGTT+AGG 0.302097 8.4:-72989622 None:intergenic
GCCACAATGGATAAAGAATA+TGG 0.304555 8.4:+72990024 MS.gene25140:CDS
ATGCTAAGTCAGTTGGATTT+GGG 0.354359 8.4:+72989680 MS.gene25140:CDS
GTAAGAAACCATTTAGATAA+AGG 0.354999 8.4:+72990081 MS.gene25140:CDS
CGGCGTTTGGCTTTAGAGTC+AGG 0.368656 8.4:+72990105 MS.gene25140:CDS
TTTAGATAAAGGTCGGCGTT+TGG 0.420224 8.4:+72990092 MS.gene25140:CDS
GAATTAATGGAAGTTGTTGA+TGG 0.438521 8.4:+72989496 None:intergenic
GATGGAGAACATTCTATTGA+TGG 0.443366 8.4:+72989520 MS.gene25140:CDS
GGAAAATAATGTGCATATGC+TGG 0.447627 8.4:-72989919 None:intergenic
ATATGAAAACATTGGTTGCT+TGG 0.449564 8.4:+72990050 MS.gene25140:CDS
ATGGAAGTTGTTGATGGGGA+TGG 0.471153 8.4:+72989502 MS.gene25140:CDS
TCCATATTCTTTATCCATTG+TGG 0.490369 8.4:-72990025 None:intergenic
CGTGTTAAAAGAATTTGTGA+TGG 0.498075 8.4:+72989847 MS.gene25140:CDS
ACTCATTATGCTAAGTCAGT+TGG 0.500199 8.4:+72989673 MS.gene25140:CDS
AATTAATGGAAGTTGTTGAT+GGG 0.514194 8.4:+72989497 None:intergenic
AGTTATGCTTGCACAAGATA+CGG 0.514711 8.4:+72989757 MS.gene25140:CDS
AAATCCACGTCCTTGTTTGA+AGG 0.523815 8.4:+72989804 MS.gene25140:CDS
ATTGAAACTTTGCACCATGT+TGG 0.528985 8.4:+72989979 MS.gene25140:CDS
TCCACGTCCTTGTTTGAAGG+TGG 0.544839 8.4:+72989807 MS.gene25140:CDS
TTATTGATTCCTCTATGGCA+AGG 0.554774 8.4:-72989940 None:intergenic
ATGGAGAACATTCTATTGAT+GGG 0.556834 8.4:+72989521 MS.gene25140:CDS
TATGGAGGATATGAAAACAT+TGG 0.557417 8.4:+72990042 MS.gene25140:CDS
AGGAGATGCAAATACAATGT+TGG 0.557447 8.4:+72990125 MS.gene25140:CDS
CCACGTCCTTGTTTGAAGGT+GGG 0.564321 8.4:+72989808 MS.gene25140:CDS
GAATGAAGAGCAAGCTGTCA+AGG 0.567338 8.4:+72989543 MS.gene25140:CDS
TCAAGAACCTAACATTGGAA+TGG 0.572602 8.4:+72989615 MS.gene25140:CDS
TGCTTGTTCTCACTCCAACA+TGG 0.579133 8.4:-72989993 None:intergenic
TTGTTTGAAGGTGGGATGTG+AGG 0.585943 8.4:+72989816 MS.gene25140:CDS
CCCACCTTCAAACAAGGACG+TGG 0.589716 8.4:-72989808 None:intergenic
TCACATCCCACCTTCAAACA+AGG 0.596792 8.4:-72989814 None:intergenic
GAAACCATTTAGATAAAGGT+CGG 0.598835 8.4:+72990085 MS.gene25140:CDS
GGTGGTCAAGAACCTAACAT+TGG 0.606305 8.4:+72989610 MS.gene25140:CDS
ACCGTTAGCCTCTAAATCAA+CGG 0.614838 8.4:-72989590 None:intergenic
ACCGTTGATTTAGAGGCTAA+CGG 0.628314 8.4:+72989589 MS.gene25140:CDS
ATTAATGGAAGTTGTTGATG+GGG 0.631609 8.4:+72989498 None:intergenic
ACAATGGATAAAGAATATGG+AGG 0.714078 8.4:+72990027 MS.gene25140:CDS
GTTGATTTAGAGGCTAACGG+TGG 0.762259 8.4:+72989592 MS.gene25140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTAAGAAACCATTTAGATAA+AGG + chr8.4:72990081-72990100 MS.gene25140:CDS 25.0%
!! AAAGAATTTGTGATGGAAAA+TGG + chr8.4:72989854-72989873 MS.gene25140:CDS 25.0%
ACAATGGATAAAGAATATGG+AGG + chr8.4:72990027-72990046 MS.gene25140:CDS 30.0%
ATGGAGAACATTCTATTGAT+GGG + chr8.4:72989521-72989540 MS.gene25140:CDS 30.0%
CGTGTTAAAAGAATTTGTGA+TGG + chr8.4:72989847-72989866 MS.gene25140:CDS 30.0%
GAAACCATTTAGATAAAGGT+CGG + chr8.4:72990085-72990104 MS.gene25140:CDS 30.0%
TATGGAGGATATGAAAACAT+TGG + chr8.4:72990042-72990061 MS.gene25140:CDS 30.0%
TCAAATTCCATTCCAATGTT+AGG - chr8.4:72989625-72989644 None:intergenic 30.0%
TCCATATTCTTTATCCATTG+TGG - chr8.4:72990028-72990047 None:intergenic 30.0%
! ATATGAAAACATTGGTTGCT+TGG + chr8.4:72990050-72990069 MS.gene25140:CDS 30.0%
!!! AAGCAAATTTTTTGCCACAA+TGG + chr8.4:72990011-72990030 MS.gene25140:CDS 30.0%
ACTCATTATGCTAAGTCAGT+TGG + chr8.4:72989673-72989692 MS.gene25140:CDS 35.0%
AGGAGATGCAAATACAATGT+TGG + chr8.4:72990125-72990144 MS.gene25140:CDS 35.0%
AGTTATGCTTGCACAAGATA+CGG + chr8.4:72989757-72989776 MS.gene25140:CDS 35.0%
ATGCTAAGTCAGTTGGATTT+GGG + chr8.4:72989680-72989699 MS.gene25140:CDS 35.0%
ATTGAAACTTTGCACCATGT+TGG + chr8.4:72989979-72989998 MS.gene25140:CDS 35.0%
GATGGAGAACATTCTATTGA+TGG + chr8.4:72989520-72989539 MS.gene25140:CDS 35.0%
GCCACAATGGATAAAGAATA+TGG + chr8.4:72990024-72990043 MS.gene25140:CDS 35.0%
GGAAAATAATGTGCATATGC+TGG - chr8.4:72989922-72989941 None:intergenic 35.0%
TATGCTAAGTCAGTTGGATT+TGG + chr8.4:72989679-72989698 MS.gene25140:CDS 35.0%
TCAAGAACCTAACATTGGAA+TGG + chr8.4:72989615-72989634 MS.gene25140:CDS 35.0%
! CATTATTTTCCTTGCCATAG+AGG + chr8.4:72989931-72989950 MS.gene25140:CDS 35.0%
!! GAGCTTTATTGATTCCTCTA+TGG - chr8.4:72989948-72989967 None:intergenic 35.0%
!! TTATTGATTCCTCTATGGCA+AGG - chr8.4:72989943-72989962 None:intergenic 35.0%
!! TTCATGTACCGTTGATTTAG+AGG + chr8.4:72989582-72989601 MS.gene25140:CDS 35.0%
AACGCCGACCTTTATCTAAA+TGG - chr8.4:72990092-72990111 None:intergenic 40.0%
CTGGAAAAAGTTCGTGATTG+TGG - chr8.4:72989903-72989922 None:intergenic 40.0%
TTTAGATAAAGGTCGGCGTT+TGG + chr8.4:72990092-72990111 MS.gene25140:CDS 40.0%
! AAATCCACGTCCTTGTTTGA+AGG + chr8.4:72989804-72989823 MS.gene25140:CDS 40.0%
! ACCGTTAGCCTCTAAATCAA+CGG - chr8.4:72989593-72989612 None:intergenic 40.0%
!! ACCGTTGATTTAGAGGCTAA+CGG + chr8.4:72989589-72989608 MS.gene25140:CDS 40.0%
GAATGAAGAGCAAGCTGTCA+AGG + chr8.4:72989543-72989562 MS.gene25140:CDS 45.0%
GGTGGTCAAGAACCTAACAT+TGG + chr8.4:72989610-72989629 MS.gene25140:CDS 45.0%
TCACATCCCACCTTCAAACA+AGG - chr8.4:72989817-72989836 None:intergenic 45.0%
TGCTTGTTCTCACTCCAACA+TGG - chr8.4:72989996-72990015 None:intergenic 45.0%
TTGTTTGAAGGTGGGATGTG+AGG + chr8.4:72989816-72989835 MS.gene25140:CDS 45.0%
! AGGACGTGGATTTTGTGAAC+TGG - chr8.4:72989797-72989816 None:intergenic 45.0%
! ATGGAAGTTGTTGATGGGGA+TGG + chr8.4:72989502-72989521 MS.gene25140:CDS 45.0%
! GTTGATTTAGAGGCTAACGG+TGG + chr8.4:72989592-72989611 MS.gene25140:CDS 45.0%
! CCACGTCCTTGTTTGAAGGT+GGG + chr8.4:72989808-72989827 MS.gene25140:CDS 50.0%
! TCCACGTCCTTGTTTGAAGG+TGG + chr8.4:72989807-72989826 MS.gene25140:CDS 50.0%
CCCACCTTCAAACAAGGACG+TGG - chr8.4:72989811-72989830 None:intergenic 55.0%
!! CGGCGTTTGGCTTTAGAGTC+AGG + chr8.4:72990105-72990124 MS.gene25140:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 72989502 72990173 72989502 ID=MS.gene25140
chr8.4 mRNA 72989502 72990173 72989502 ID=MS.gene25140.t1;Parent=MS.gene25140
chr8.4 exon 72989502 72990173 72989502 ID=MS.gene25140.t1.exon1;Parent=MS.gene25140.t1
chr8.4 CDS 72989502 72990173 72989502 ID=cds.MS.gene25140.t1;Parent=MS.gene25140.t1
Gene Sequence

>MS.gene25140

ATGGAAGTTGTTGATGGGGATGGAGAACATTCTATTGATGGGAATGAAGAGCAAGCTGTCAAGGAGATTGATGACAATGATTCATGTACCGTTGATTTAGAGGCTAACGGTGGTCAAGAACCTAACATTGGAATGGAATTTGACTCACAAGAAAATGCTTATTCATTCTACACTCATTATGCTAAGTCAGTTGGATTTGGGATATCAATTAAAAATAGTCGTCGTTCAAAGATTTTTAGAGAGTTAATTGATGTAAGTTATGCTTGCACAAGATACGGCAAGAAGCGAGAATCCAGTTCACAAAATCCACGTCCTTGTTTGAAGGTGGGATGTGAGGCTAGTTTACGTGTTAAAAGAATTTGTGATGGAAAATGGATTGTTCATAGCTTCGTTAAAGACCACAATCACGAACTTTTTCCAGCATATGCACATTATTTTCCTTGCCATAGAGGAATCAATAAAGCTCAAAAGCATTCTATTGAAACTTTGCACCATGTTGGAGTGAGAACAAGCAAATTTTTTGCCACAATGGATAAAGAATATGGAGGATATGAAAACATTGGTTGCTTGGAGAAAGATGTAAGAAACCATTTAGATAAAGGTCGGCGTTTGGCTTTAGAGTCAGGAGATGCAAATACAATGTTGGATTGTTTTATGCTCATGCAAAAGTAG

Protein sequence

>MS.gene25140.t1

MEVVDGDGEHSIDGNEEQAVKEIDDNDSCTVDLEANGGQEPNIGMEFDSQENAYSFYTHYAKSVGFGISIKNSRRSKIFRELIDVSYACTRYGKKRESSSQNPRPCLKVGCEASLRVKRICDGKWIVHSFVKDHNHELFPAYAHYFPCHRGINKAQKHSIETLHHVGVRTSKFFATMDKEYGGYENIGCLEKDVRNHLDKGRRLALESGDANTMLDCFMLMQK