AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25193.t1 MTR_1g047060 71.875 96 24 1 111 206 1 93 3.07e-41 136
MS.gene25193.t1 MTR_6g477900 33.708 178 86 8 4 167 6 165 6.33e-19 83.2
MS.gene25193.t1 MTR_2g086880 36.486 148 77 7 6 146 20 157 3.25e-18 83.6
MS.gene25193.t1 MTR_2g086880 35.333 150 76 7 6 146 20 157 3.62e-18 83.6
MS.gene25193.t1 MTR_5g021710 33.758 157 86 7 2 147 6 155 3.51e-16 77.0
MS.gene25193.t1 MTR_4g098630 36.478 159 83 8 2 147 4 157 5.95e-16 75.9
MS.gene25193.t1 MTR_3g093050 30.769 182 100 7 4 171 22 191 8.01e-16 76.6
MS.gene25193.t1 MTR_2g064090 34.615 156 84 7 4 147 6 155 1.08e-15 75.5
MS.gene25193.t1 MTR_3g093050 30.769 182 100 7 4 171 22 191 1.17e-15 76.3
MS.gene25193.t1 MTR_5g014300 33.962 159 86 7 2 146 4 157 1.53e-15 75.1
MS.gene25193.t1 MTR_4g094302 30.189 159 86 7 4 148 11 158 2.09e-15 72.4
MS.gene25193.t1 MTR_8g467490 30.247 162 87 6 6 147 16 171 3.93e-15 74.3
MS.gene25193.t1 MTR_1g097300 34.848 132 76 4 6 133 21 146 5.60e-15 74.3
MS.gene25193.t1 MTR_5g069030 34.177 158 81 7 4 147 25 173 6.82e-15 73.9
MS.gene25193.t1 MTR_4g089135 37.778 135 72 5 4 133 15 142 1.16e-14 72.8
MS.gene25193.t1 MTR_4g094302 33.077 130 77 5 4 133 11 130 1.38e-14 69.7
MS.gene25193.t1 MTR_5g040420 31.056 161 86 7 6 147 16 170 1.51e-14 72.4
MS.gene25193.t1 MTR_8g076110 33.333 159 84 8 2 147 4 153 1.62e-14 72.0
MS.gene25193.t1 MTR_1g090720 35.583 163 78 7 4 147 42 196 1.88e-14 72.4
MS.gene25193.t1 MTR_4g036030 32.484 157 88 8 2 147 5 154 1.13e-13 69.7
MS.gene25193.t1 MTR_4g101680 31.847 157 89 7 2 147 5 154 2.05e-13 69.3
MS.gene25193.t1 MTR_6g011860 31.707 164 85 8 4 147 15 171 2.56e-13 68.9
MS.gene25193.t1 MTR_5g076850 30.114 176 98 9 4 163 6 172 3.73e-13 68.9
MS.gene25193.t1 MTR_3g064580 32.704 159 83 9 4 147 6 155 4.15e-13 68.6
MS.gene25193.t1 MTR_0036s0150 30.323 155 89 6 6 148 7 154 4.84e-13 68.6
MS.gene25193.t1 MTR_7g100990 32.609 138 79 4 4 135 11 140 7.42e-13 67.4
MS.gene25193.t1 MTR_4g081870 28.571 161 88 6 4 147 9 159 3.17e-12 65.1
MS.gene25193.t1 MTR_4g075980 32.450 151 85 7 6 147 17 159 5.22e-12 64.7
MS.gene25193.t1 MTR_3g109340 30.968 155 89 6 4 147 22 169 6.31e-12 64.7
MS.gene25193.t1 MTR_8g023860 30.719 153 90 5 4 147 12 157 6.59e-12 65.1
MS.gene25193.t1 MTR_2g079990 31.646 158 83 8 4 147 16 162 9.44e-12 64.3
MS.gene25193.t1 MTR_6g012670 26.901 171 95 8 4 155 20 179 1.16e-11 63.9
MS.gene25193.t1 MTR_2157s0010 28.824 170 84 9 4 147 8 166 1.31e-11 63.2
MS.gene25193.t1 MTR_3g109340 30.519 154 90 6 4 147 22 168 1.73e-11 63.5
MS.gene25193.t1 MTR_8g059170 31.875 160 82 8 4 147 14 162 2.97e-11 62.8
MS.gene25193.t1 MTR_1g069805 28.662 157 91 7 2 147 8 154 4.94e-11 61.6
MS.gene25193.t1 MTR_7g085220 27.976 168 89 7 4 147 8 167 6.06e-11 62.0
MS.gene25193.t1 MTR_2g079990 33.813 139 74 7 4 135 16 143 9.21e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25193.t1 AT3G49530 33.333 192 107 9 1 179 10 193 4.17e-20 88.6
MS.gene25193.t1 AT3G49530 33.333 192 107 9 1 179 10 193 4.89e-20 89.0
MS.gene25193.t1 AT5G24590 37.500 160 80 7 1 147 10 162 5.19e-19 85.9
MS.gene25193.t1 AT4G36160 35.849 159 84 7 4 151 14 165 6.22e-19 85.1
MS.gene25193.t1 AT4G36160 35.849 159 84 7 4 151 10 161 6.55e-19 85.1
MS.gene25193.t1 AT4G36160 35.849 159 84 7 4 151 10 161 6.55e-19 85.1
MS.gene25193.t1 AT3G10490 35.465 172 89 7 4 159 27 192 2.33e-18 81.6
MS.gene25193.t1 AT3G17730 32.911 158 83 7 4 147 6 154 3.43e-17 78.6
MS.gene25193.t1 AT3G10490 36.538 156 81 6 4 147 27 176 3.58e-17 80.5
MS.gene25193.t1 AT1G33060 34.545 165 83 7 1 150 21 175 4.72e-17 80.5
MS.gene25193.t1 AT1G33060 34.545 165 83 7 1 150 21 175 5.15e-17 80.1
MS.gene25193.t1 AT3G10480 33.690 187 95 9 4 172 27 202 1.14e-16 79.0
MS.gene25193.t1 AT4G17980 34.591 159 85 7 2 146 4 157 1.15e-16 77.4
MS.gene25193.t1 AT2G18060 34.194 155 84 7 4 147 9 156 1.44e-16 78.2
MS.gene25193.t1 AT2G18060 34.194 155 84 7 4 147 9 156 1.44e-16 78.2
MS.gene25193.t1 AT4G17980 34.591 159 85 7 2 146 4 157 2.35e-16 77.0
MS.gene25193.t1 AT5G64530 32.796 186 87 9 4 165 3 174 3.89e-16 74.3
MS.gene25193.t1 AT2G27300 32.237 152 88 6 1 146 11 153 3.97e-16 76.6
MS.gene25193.t1 AT3G10480 33.333 186 96 9 4 172 27 201 4.07e-16 77.4
MS.gene25193.t1 AT1G26870 34.940 166 80 8 3 147 15 173 4.43e-16 77.0
MS.gene25193.t1 AT5G64530 36.364 132 70 4 4 135 3 120 4.88e-16 73.6
MS.gene25193.t1 AT1G69490 32.099 162 85 7 2 147 7 159 5.58e-16 75.5
MS.gene25193.t1 AT5G04410 32.903 155 87 5 4 147 9 157 1.38e-15 75.9
MS.gene25193.t1 AT4G35580 33.333 162 89 8 1 150 6 160 2.76e-15 75.1
MS.gene25193.t1 AT4G35580 32.099 162 91 6 1 150 6 160 2.78e-15 75.1
MS.gene25193.t1 AT4G35580 32.099 162 91 6 1 150 6 160 3.59e-15 74.7
MS.gene25193.t1 AT2G24430 32.484 157 84 7 4 147 16 163 4.37e-15 73.6
MS.gene25193.t1 AT2G24430 32.484 157 84 7 4 147 16 163 4.37e-15 73.6
MS.gene25193.t1 AT5G46590 32.911 158 87 6 2 146 4 155 6.50e-15 72.8
MS.gene25193.t1 AT3G44290 31.034 145 89 6 6 146 16 153 1.00e-14 71.2
MS.gene25193.t1 AT5G22290 33.562 146 85 7 6 146 23 161 1.05e-14 72.8
MS.gene25193.t1 AT1G32510 32.099 162 89 6 2 146 4 161 1.61e-14 71.6
MS.gene25193.t1 AT1G54330 34.868 152 85 6 4 147 6 151 2.96e-14 71.2
MS.gene25193.t1 AT5G62380 31.875 160 87 8 1 147 4 154 3.59e-14 71.2
MS.gene25193.t1 AT5G62380 31.875 160 87 8 1 147 4 154 3.59e-14 71.2
MS.gene25193.t1 AT5G09330 31.395 172 100 6 2 161 4 169 3.59e-14 71.6
MS.gene25193.t1 AT5G09330 31.395 172 100 6 2 161 4 169 3.59e-14 71.6
MS.gene25193.t1 AT5G09330 31.395 172 100 6 2 161 4 169 3.59e-14 71.6
MS.gene25193.t1 AT5G09330 31.395 172 100 6 2 161 4 169 3.59e-14 71.6
MS.gene25193.t1 AT5G09330 31.395 172 100 6 2 161 4 169 3.59e-14 71.6
MS.gene25193.t1 AT3G44290 31.034 145 89 6 6 146 16 153 4.16e-14 70.9
MS.gene25193.t1 AT5G46590 33.813 139 81 5 2 135 4 136 8.85e-14 69.7
MS.gene25193.t1 AT1G62700 33.750 160 82 10 2 147 5 154 1.15e-13 70.1
MS.gene25193.t1 AT1G62700 33.750 160 82 10 2 147 5 154 1.15e-13 70.1
MS.gene25193.t1 AT5G39610 30.000 160 85 7 4 147 20 168 1.41e-13 68.9
MS.gene25193.t1 AT3G29035 28.333 180 89 7 4 154 24 192 1.16e-12 66.6
MS.gene25193.t1 AT4G27410 31.013 158 84 8 4 147 14 160 1.37e-12 66.2
MS.gene25193.t1 AT3G15510 31.447 159 87 7 10 162 23 165 4.62e-12 65.1
MS.gene25193.t1 AT1G61110 35.036 137 76 6 4 135 16 144 5.82e-12 64.7
MS.gene25193.t1 AT5G04395 30.612 147 82 7 4 134 28 170 9.60e-12 62.4
MS.gene25193.t1 AT5G61430 29.375 160 86 7 4 147 16 164 1.25e-11 63.9
MS.gene25193.t1 AT1G76420 27.950 161 88 6 4 148 22 170 1.68e-11 63.5
MS.gene25193.t1 AT5G07680 30.435 161 83 8 4 147 17 165 2.32e-11 63.2
MS.gene25193.t1 AT5G07680 30.435 161 83 8 4 147 3 151 2.65e-11 62.8
MS.gene25193.t1 AT3G15500 29.936 157 87 7 4 147 14 160 3.20e-11 62.4
MS.gene25193.t1 AT1G79580 31.818 154 89 6 4 147 17 164 4.27e-11 62.4
MS.gene25193.t1 AT1G79580 31.818 154 89 6 4 147 17 164 4.27e-11 62.4
MS.gene25193.t1 AT1G79580 31.818 154 89 6 4 147 17 164 4.27e-11 62.4
MS.gene25193.t1 AT1G79580 31.818 154 89 6 4 147 17 164 4.27e-11 62.4
MS.gene25193.t1 AT1G79580 31.818 154 89 6 4 147 17 164 4.27e-11 62.4
MS.gene25193.t1 AT1G52890 31.056 161 80 8 4 147 14 160 6.29e-11 61.6
MS.gene25193.t1 AT4G27410 29.240 171 87 8 4 147 14 177 8.33e-11 61.2
MS.gene25193.t1 AT3G18400 31.410 156 87 8 4 148 5 151 8.82e-11 61.2

Find 65 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTTAAGCTTGCAATTA+TGG 0.141545 1.3:-17229561 MS.gene25193:CDS
GGCACTGCAGGACTGGATTT+TGG 0.202792 1.3:-17230939 MS.gene25193:CDS
TCCCTCTTTATGTTCTATCT+TGG 0.300819 1.3:-17230853 MS.gene25193:CDS
CTGGTAGTAGTGATGGTGGT+GGG 0.303392 1.3:-17230502 MS.gene25193:CDS
TGGAGATGACAATGTGGTTT+TGG 0.323180 1.3:-17230884 MS.gene25193:CDS
ATGGATCTGGGTGCAGAGTT+TGG 0.323843 1.3:-17230602 MS.gene25193:CDS
TCCATGGTTCTGACCTCATT+AGG 0.324328 1.3:-17231077 MS.gene25193:CDS
GCTATGCTTCCACCTGGTGC+TGG 0.351315 1.3:-17231171 MS.gene25193:CDS
CCATGGTTCTGACCTCATTA+GGG 0.355765 1.3:-17231076 MS.gene25193:CDS
TTGCCCTCCAGCAATGGATC+TGG 0.371581 1.3:-17230615 MS.gene25193:CDS
GGCATTACTCGAGACATTAA+TGG 0.390991 1.3:-17230907 MS.gene25193:CDS
TGATGTCACTCGAAAACTTA+AGG 0.396017 1.3:-17230534 MS.gene25193:CDS
GAGAGACGGCACTGCAGGAC+TGG 0.427179 1.3:-17230946 MS.gene25193:CDS
CCCACTGACGAGATACTTGT+TGG 0.432459 1.3:-17231141 MS.gene25193:CDS
AAAACACGGCACACAACAAA+AGG 0.436311 1.3:+17230641 None:intergenic
GCTGGTAGTAGTGATGGTGG+TGG 0.451886 1.3:-17230503 MS.gene25193:CDS
GATGTCACTCGAAAACTTAA+GGG 0.453296 1.3:-17230533 MS.gene25193:CDS
GCTGGGGCTGGTAGTAGTGA+TGG 0.454213 1.3:-17230509 MS.gene25193:CDS
CTATGCTTCCACCTGGTGCT+GGG 0.456622 1.3:-17231170 MS.gene25193:CDS
TCTGCACCCAGATCCATTGC+TGG 0.460133 1.3:+17230608 None:intergenic
TACTTGTTGGCTACTACCTT+AGG 0.477557 1.3:-17231128 MS.gene25193:CDS
GGTGGTGGGGCTGCTGAGCA+AGG 0.481889 1.3:-17230488 MS.gene25193:CDS
CGATCGGGTCATACTCATAC+AGG 0.485419 1.3:+17231046 None:intergenic
TACTGCTTCACTGCCAAAGT+TGG 0.486292 1.3:-17230973 MS.gene25193:CDS
CCGTCTCTCACTGCCAACTT+TGG 0.487719 1.3:+17230960 None:intergenic
TTCCAAGATAGAACATAAAG+AGG 0.498797 1.3:+17230851 None:intergenic
AGGACGCTGTCAGAACAAAC+TGG 0.499874 1.3:-17230819 MS.gene25193:CDS
TGGTAGTAGTGATGGTGGTG+GGG 0.505536 1.3:-17230501 MS.gene25193:CDS
TGCCCTCCAGCAATGGATCT+GGG 0.507906 1.3:-17230614 MS.gene25193:CDS
GCAGTGAGAGACGGCACTGC+AGG 0.521491 1.3:-17230951 MS.gene25193:CDS
TGCATTGGCTGACAAGCCTC+AGG 0.522270 1.3:-17230782 MS.gene25193:intron
GCCAACAAGTATCTCGTCAG+TGG 0.527034 1.3:+17231140 None:intergenic
ATTGCAAGCTTAAATTGCTC+CGG 0.527174 1.3:+17229566 None:intergenic
ACCTTCTTCAACATCAGATA+AGG 0.528743 1.3:+17230577 None:intergenic
ATTGTCGATGGTATAGTAGA+AGG 0.534238 1.3:+17231008 None:intergenic
GTGGGGAGAAACCCAGCACC+AGG 0.542796 1.3:+17231159 None:intergenic
GAACAGGTTCAGAATTTCCA+TGG 0.547755 1.3:-17231093 MS.gene25193:CDS
GAGGTATATGACAATTCGAT+CGG 0.559279 1.3:+17231030 None:intergenic
CCACGTGGAAGCTATAACGC+CGG 0.563222 1.3:-17229585 MS.gene25193:CDS
ACAGGTTCAGCTCCCTAATG+AGG 0.565571 1.3:+17231064 None:intergenic
GCAATGGCTATGCTTCCACC+TGG 0.574224 1.3:-17231177 MS.gene25193:CDS
AGGTATATGACAATTCGATC+GGG 0.577727 1.3:+17231031 None:intergenic
CCAACAAGTATCTCGTCAGT+GGG 0.578284 1.3:+17231141 None:intergenic
CTATCTTGGAAATTCTGTGA+AGG 0.589080 1.3:-17230839 MS.gene25193:CDS
GCACCCAGATCCATTGCTGG+AGG 0.602073 1.3:+17230611 None:intergenic
ACTATACCATCGACAATCGT+AGG 0.602081 1.3:-17231002 MS.gene25193:CDS
GGGGCTGGTAGTAGTGATGG+TGG 0.602818 1.3:-17230506 MS.gene25193:CDS
CCCTAATGAGGTCAGAACCA+TGG 0.604440 1.3:+17231076 None:intergenic
ATGTCACTCGAAAACTTAAG+GGG 0.610861 1.3:-17230532 MS.gene25193:CDS
CACCCAGATCCATTGCTGGA+GGG 0.613242 1.3:+17230612 None:intergenic
ATTTAAGCTTGCAATTATGG+AGG 0.617003 1.3:-17229558 MS.gene25193:CDS
GTCGATGGTATAGTAGAAGG+AGG 0.618515 1.3:+17231011 None:intergenic
GCCTTATCTGATGTTGAAGA+AGG 0.619001 1.3:-17230578 MS.gene25193:CDS
CCAAAGTTGGCAGTGAGAGA+CGG 0.623168 1.3:-17230960 MS.gene25193:CDS
TCCAAGATAGAACATAAAGA+GGG 0.626576 1.3:+17230852 None:intergenic
GAATTGCCTACGATTGTCGA+TGG 0.629431 1.3:+17230996 None:intergenic
GTTGAAGAAGGTGATGCTGC+TGG 0.630740 1.3:-17230566 MS.gene25193:CDS
GGGAGAAACCCAGCACCAGG+TGG 0.671634 1.3:+17231162 None:intergenic
GATTATGTACGAGTATGCAT+TGG 0.682043 1.3:-17230797 MS.gene25193:CDS
ATTACTCGAGACATTAATGG+TGG 0.700868 1.3:-17230904 MS.gene25193:CDS
TGTCACTCGAAAACTTAAGG+GGG 0.705876 1.3:-17230531 MS.gene25193:CDS
CCGGCGTTATAGCTTCCACG+TGG 0.714144 1.3:+17229585 None:intergenic
TGACAATGTTGTCTTCCACG+TGG 0.721449 1.3:-17229600 MS.gene25193:CDS
CAACAAGTATCTCGTCAGTG+GGG 0.741470 1.3:+17231142 None:intergenic
TAATGGTGGAGATGACAATG+TGG 0.781339 1.3:-17230890 MS.gene25193:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATTTAAAGAAGAAAAAA+AGG + chr1.3:17230935-17230954 None:intergenic 15.0%
!! GATAAAATTTATCAAAACTT+TGG - chr1.3:17230559-17230578 MS.gene25193:CDS 15.0%
!!! ATTAAACCATTTTTTAAACT+CGG + chr1.3:17230659-17230678 None:intergenic 15.0%
!!! TGTTTGTTTTAATGTTTATT+AGG - chr1.3:17231076-17231095 MS.gene25193:CDS 15.0%
!!! TTTTTATATTATTATTGTGC+AGG - chr1.3:17230052-17230071 MS.gene25193:intron 15.0%
!! AAATTTCCGAGTTTAAAAAA+TGG - chr1.3:17230650-17230669 MS.gene25193:intron 20.0%
!!! CAACTTTTCATTTCATTTTA+TGG - chr1.3:17231015-17231034 MS.gene25193:CDS 20.0%
!!! TCAATGATTTTATGTTTGTA+TGG - chr1.3:17230471-17230490 MS.gene25193:CDS 20.0%
! AAAAAATACCATCACATGAA+TGG + chr1.3:17230773-17230792 None:intergenic 25.0%
! ACATAAAATCATTGATGTTG+CGG + chr1.3:17230466-17230485 None:intergenic 25.0%
! ATAAGATCAACAACATTAAG+TGG + chr1.3:17230707-17230726 None:intergenic 25.0%
! ATAATCAATAGCAAAGATTG+AGG + chr1.3:17230802-17230821 None:intergenic 25.0%
! ATATGTATTTGACTGCTATT+GGG - chr1.3:17230404-17230423 MS.gene25193:intron 25.0%
!!! CTTCTTTAAATTTGTTACCA+TGG - chr1.3:17230941-17230960 MS.gene25193:CDS 25.0%
!!! TGGTTTAATTTCAAGTTTTC+AGG - chr1.3:17230579-17230598 MS.gene25193:CDS 25.0%
ATACAAAGACAGATCAACAA+TGG + chr1.3:17230308-17230327 None:intergenic 30.0%
ATTATCCCACAAAATTGACA+TGG + chr1.3:17230616-17230635 None:intergenic 30.0%
ATTTAAGCTTGCAATTATGG+AGG - chr1.3:17231160-17231179 MS.gene25193:CDS 30.0%
CATATGTATTTGACTGCTAT+TGG - chr1.3:17230403-17230422 MS.gene25193:intron 30.0%
GCAATTTAAGCTTGCAATTA+TGG - chr1.3:17231157-17231176 MS.gene25193:CDS 30.0%
GTTGTCATAGTAACAAAATG+TGG - chr1.3:17230508-17230527 MS.gene25193:CDS 30.0%
TCCAAGATAGAACATAAAGA+GGG + chr1.3:17229869-17229888 None:intergenic 30.0%
TTATCCCACAAAATTGACAT+GGG + chr1.3:17230615-17230634 None:intergenic 30.0%
TTCCAAGATAGAACATAAAG+AGG + chr1.3:17229870-17229889 None:intergenic 30.0%
! ATGGAATATATTTTACCTGC+AGG + chr1.3:17230289-17230308 None:intergenic 30.0%
! ATTTTATGGTAGAGACAAGA+AGG - chr1.3:17231029-17231048 MS.gene25193:CDS 30.0%
! GTCAATTTTGTGGGATAATT+TGG - chr1.3:17230617-17230636 MS.gene25193:CDS 30.0%
! TAATGATGATGAATTTGCCA+TGG + chr1.3:17230961-17230980 None:intergenic 30.0%
! TATTCCCATGTCAATTTTGT+GGG - chr1.3:17230608-17230627 MS.gene25193:CDS 30.0%
! TCACATTTCACAGATTTTCA+AGG - chr1.3:17230260-17230279 MS.gene25193:intron 30.0%
! TGGAATATATTTTACCTGCA+GGG + chr1.3:17230288-17230307 None:intergenic 30.0%
! TTATTCCCATGTCAATTTTG+TGG - chr1.3:17230607-17230626 MS.gene25193:CDS 30.0%
AACTCAATCCATTCATGTGA+TGG - chr1.3:17230762-17230781 MS.gene25193:intron 35.0%
ACCTTCTTCAACATCAGATA+AGG + chr1.3:17230144-17230163 None:intergenic 35.0%
AGGTATATGACAATTCGATC+GGG + chr1.3:17229690-17229709 None:intergenic 35.0%
ATGTCACTCGAAAACTTAAG+GGG - chr1.3:17230186-17230205 MS.gene25193:intron 35.0%
ATTACTCGAGACATTAATGG+TGG - chr1.3:17229814-17229833 MS.gene25193:intron 35.0%
ATTGCAAGCTTAAATTGCTC+CGG + chr1.3:17231155-17231174 None:intergenic 35.0%
ATTGTCGATGGTATAGTAGA+AGG + chr1.3:17229713-17229732 None:intergenic 35.0%
CTATCTTGGAAATTCTGTGA+AGG - chr1.3:17229879-17229898 MS.gene25193:intron 35.0%
GAGGTATATGACAATTCGAT+CGG + chr1.3:17229691-17229710 None:intergenic 35.0%
GATGTCACTCGAAAACTTAA+GGG - chr1.3:17230185-17230204 MS.gene25193:intron 35.0%
GATTATGTACGAGTATGCAT+TGG - chr1.3:17229921-17229940 MS.gene25193:intron 35.0%
TAGGAACAAAAATAGCGAAC+AGG - chr1.3:17229609-17229628 MS.gene25193:CDS 35.0%
TCCCTCTTTATGTTCTATCT+TGG - chr1.3:17229865-17229884 MS.gene25193:intron 35.0%
TGATGTCACTCGAAAACTTA+AGG - chr1.3:17230184-17230203 MS.gene25193:intron 35.0%
TTTCACAAATCTCATACCTG+AGG + chr1.3:17229955-17229974 None:intergenic 35.0%
! ACTGGATTTTGGATGAAGAA+GGG - chr1.3:17229790-17229809 MS.gene25193:intron 35.0%
!!! GTTCGCTATTTTTGTTCCTA+AGG + chr1.3:17229609-17229628 None:intergenic 35.0%
AAAACACGGCACACAACAAA+AGG + chr1.3:17230080-17230099 None:intergenic 40.0%
ACTATACCATCGACAATCGT+AGG - chr1.3:17229716-17229735 MS.gene25193:intron 40.0%
GAACAGGTTCAGAATTTCCA+TGG - chr1.3:17229625-17229644 MS.gene25193:intron 40.0%
GCCTTATCTGATGTTGAAGA+AGG - chr1.3:17230140-17230159 MS.gene25193:intron 40.0%
GGCATTACTCGAGACATTAA+TGG - chr1.3:17229811-17229830 MS.gene25193:intron 40.0%
TGTCACTCGAAAACTTAAGG+GGG - chr1.3:17230187-17230206 MS.gene25193:intron 40.0%
! GACTGGATTTTGGATGAAGA+AGG - chr1.3:17229789-17229808 MS.gene25193:intron 40.0%
! GGAGATGACAATGTGGTTTT+GGG - chr1.3:17229835-17229854 MS.gene25193:intron 40.0%
! TAATGGTGGAGATGACAATG+TGG - chr1.3:17229828-17229847 MS.gene25193:intron 40.0%
! TACTTGTTGGCTACTACCTT+AGG - chr1.3:17229590-17229609 MS.gene25193:CDS 40.0%
! TGGAGATGACAATGTGGTTT+TGG - chr1.3:17229834-17229853 MS.gene25193:intron 40.0%
CAACAAGTATCTCGTCAGTG+GGG + chr1.3:17229579-17229598 None:intergenic 45.0%
CCAACAAGTATCTCGTCAGT+GGG + chr1.3:17229580-17229599 None:intergenic 45.0%
CTGGAGGGCAATCAAAAACA+CGG + chr1.3:17230094-17230113 None:intergenic 45.0%
GAATTGCCTACGATTGTCGA+TGG + chr1.3:17229725-17229744 None:intergenic 45.0%
GTCGATGGTATAGTAGAAGG+AGG + chr1.3:17229710-17229729 None:intergenic 45.0%
TACTGCTTCACTGCCAAAGT+TGG - chr1.3:17229745-17229764 MS.gene25193:intron 45.0%
TGACAATGTTGTCTTCCACG+TGG - chr1.3:17231118-17231137 MS.gene25193:CDS 45.0%
!! CCATGGTTCTGACCTCATTA+GGG - chr1.3:17229642-17229661 MS.gene25193:intron 45.0%
!! TCCATGGTTCTGACCTCATT+AGG - chr1.3:17229641-17229660 MS.gene25193:intron 45.0%
!! TTTTGATTGCCCTCCAGCAA+TGG - chr1.3:17230097-17230116 MS.gene25193:intron 45.0%
ACAGGTTCAGCTCCCTAATG+AGG + chr1.3:17229657-17229676 None:intergenic 50.0%
ACTCGAAAACTTAAGGGGGC+TGG - chr1.3:17230191-17230210 MS.gene25193:intron 50.0%
AGGACGCTGTCAGAACAAAC+TGG - chr1.3:17229899-17229918 MS.gene25193:intron 50.0%
ATGGATCTGGGTGCAGAGTT+TGG - chr1.3:17230116-17230135 MS.gene25193:intron 50.0%
CCAAAGTTGGCAGTGAGAGA+CGG - chr1.3:17229758-17229777 MS.gene25193:intron 50.0%
CCCACTGACGAGATACTTGT+TGG - chr1.3:17229577-17229596 MS.gene25193:CDS 50.0%
CCCTAATGAGGTCAGAACCA+TGG + chr1.3:17229645-17229664 None:intergenic 50.0%
CGATCGGGTCATACTCATAC+AGG + chr1.3:17229675-17229694 None:intergenic 50.0%
CTCGAAAACTTAAGGGGGCT+GGG - chr1.3:17230192-17230211 MS.gene25193:intron 50.0%
GCCAACAAGTATCTCGTCAG+TGG + chr1.3:17229581-17229600 None:intergenic 50.0%
GTTGAAGAAGGTGATGCTGC+TGG - chr1.3:17230152-17230171 MS.gene25193:intron 50.0%
TCGAAAACTTAAGGGGGCTG+GGG - chr1.3:17230193-17230212 MS.gene25193:intron 50.0%
!! AGATTTTCAAGGCTCCCTGC+AGG - chr1.3:17230271-17230290 MS.gene25193:intron 50.0%
!! CTGGTAGTAGTGATGGTGGT+GGG - chr1.3:17230216-17230235 MS.gene25193:intron 50.0%
!! TGGTAGTAGTGATGGTGGTG+GGG - chr1.3:17230217-17230236 MS.gene25193:intron 50.0%
CACCCAGATCCATTGCTGGA+GGG + chr1.3:17230109-17230128 None:intergenic 55.0%
CCACGTGGAAGCTATAACGC+CGG - chr1.3:17231133-17231152 MS.gene25193:CDS 55.0%
CCGTCTCTCACTGCCAACTT+TGG + chr1.3:17229761-17229780 None:intergenic 55.0%
CTATGCTTCCACCTGGTGCT+GGG - chr1.3:17229548-17229567 MS.gene25193:CDS 55.0%
TCTGCACCCAGATCCATTGC+TGG + chr1.3:17230113-17230132 None:intergenic 55.0%
TGCCCTCCAGCAATGGATCT+GGG - chr1.3:17230104-17230123 MS.gene25193:intron 55.0%
TTGCCCTCCAGCAATGGATC+TGG - chr1.3:17230103-17230122 MS.gene25193:intron 55.0%
! GGCACTGCAGGACTGGATTT+TGG - chr1.3:17229779-17229798 MS.gene25193:intron 55.0%
!! GCTGGTAGTAGTGATGGTGG+TGG - chr1.3:17230215-17230234 MS.gene25193:intron 55.0%
!! TGCATTGGCTGACAAGCCTC+AGG - chr1.3:17229936-17229955 MS.gene25193:intron 55.0%
AAACTTAAGGGGGCTGGGGC+TGG - chr1.3:17230197-17230216 MS.gene25193:intron 60.0%
CCGGCGTTATAGCTTCCACG+TGG + chr1.3:17231136-17231155 None:intergenic 60.0%
GCACCCAGATCCATTGCTGG+AGG + chr1.3:17230110-17230129 None:intergenic 60.0%
GCTATGCTTCCACCTGGTGC+TGG - chr1.3:17229547-17229566 MS.gene25193:CDS 60.0%
! GCTGGGGCTGGTAGTAGTGA+TGG - chr1.3:17230209-17230228 MS.gene25193:intron 60.0%
! GGGGCTGGTAGTAGTGATGG+TGG - chr1.3:17230212-17230231 MS.gene25193:intron 60.0%
GAGAGACGGCACTGCAGGAC+TGG - chr1.3:17229772-17229791 MS.gene25193:intron 65.0%
GCAGTGAGAGACGGCACTGC+AGG - chr1.3:17229767-17229786 MS.gene25193:intron 65.0%
GGGAGAAACCCAGCACCAGG+TGG + chr1.3:17229559-17229578 None:intergenic 65.0%
GTGGGGAGAAACCCAGCACC+AGG + chr1.3:17229562-17229581 None:intergenic 65.0%
!! GGTGGTGGGGCTGCTGAGCA+AGG - chr1.3:17230230-17230249 MS.gene25193:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 17229544 17231196 17229544 ID=MS.gene25193
chr1.3 mRNA 17229544 17231196 17229544 ID=MS.gene25193.t1;Parent=MS.gene25193
chr1.3 exon 17230783 17231196 17230783 ID=MS.gene25193.t1.exon1;Parent=MS.gene25193.t1
chr1.3 CDS 17230783 17231196 17230783 ID=cds.MS.gene25193.t1;Parent=MS.gene25193.t1
chr1.3 exon 17230448 17230666 17230448 ID=MS.gene25193.t1.exon2;Parent=MS.gene25193.t1
chr1.3 CDS 17230448 17230666 17230448 ID=cds.MS.gene25193.t1;Parent=MS.gene25193.t1
chr1.3 exon 17229544 17229642 17229544 ID=MS.gene25193.t1.exon3;Parent=MS.gene25193.t1
chr1.3 CDS 17229544 17229642 17229544 ID=cds.MS.gene25193.t1;Parent=MS.gene25193.t1
Gene Sequence

>MS.gene25193

ATGGCTATGCTTCCACCTGGTGCTGGGTTTCTCCCCACTGACGAGATACTTGTTGGCTACTACCTTAGGAACAAAAATAGCGAACAGGTTCAGAATTTCCATGGTTCTGACCTCATTAGGGAGCTGAACCTGTATGAGTATGACCCGATCGAATTGTCATATACCTCCTTCTACTATACCATCGACAATCGTAGGCAATTCTACTGCTTCACTGCCAAAGTTGGCAGTGAGAGACGGCACTGCAGGACTGGATTTTGGATGAAGAAGGGCATTACTCGAGACATTAATGGTGGAGATGACAATGTGGTTTTGGGTCAAAAGTCCCTCTTTATGTTCTATCTTGGAAATTCTGTGAAGGACGCTGTCAGAACAAACTGGATTATGTACGAGTATGCATTGGCTGACAAGCCTCAGGTATGAGATTTGTGAAAACTAACTTTCTCTTTGTTCGTTATTTCATCAATGATTAATTATGTATTGATAACTACTTATTTATTTTTTATTTTATTTTTTATATTATTATTGTGCAGGCTCCTTTTGTTGTGTGCCGTGTTTTTGATTGCCCTCCAGCAATGGATCTGGGTGCAGAGTTTGGAGCCTTATCTGATGTTGAAGAAGGTGATGCTGCTGGTGATGACTCTGATGTCACTCGAAAACTTAAGGGGGCTGGGGCTGGTAGTAGTGATGGTGGTGGGGCTGCTGAGCAAGGTGATGCATCACATTTCACAGATTTTCAAGGCTCCCTGCAGGTAAAATATATTCCATTGTTGATCTGTCTTTGTATTTTGTTGTCAATTAGAATTTTAGAATAGTTGAATGTGTCGTAAATCATAATTGATTTTTGTCTGTTTTTGTGTTGCATATGTATTTGACTGCTATTGGGAATAAGTTTTAATAAAATGTTGCAATTGAATGTGAGCCGCAACATCAATGATTTTATGTTTGTATGGAAAAAATTTAAATTGTTGTCATAGTAACAAAATGTGGCGATTGCATCGCAATCATTGATAGTGTAGATAAAATTTATCAAAACTTTGGTTTAATTTCAAGTTTTCAGGTTTGATTATTCCCATGTCAATTTTGTGGGATAATTTGGCTTCTTTCAAAAATTTCCGAGTTTAAAAAATGGTTTAATTAGAGATGTTATTATTGAGAGTCAACCACTTAATGTTGTTGATCTTATTTGCAACTGATTTTTTAAAATTTCAACAATTTTACAACTCAATCCATTCATGTGATGGTATTTTTTTAATTGCCTCAATCTTTGCTATTGATTATAGCATGTCAAAATGTCAACTTGTGTGTAGAAATTAGTGATAGTAATTTTCAAAACATATAACAATTGATTAGTTAACGTAGCAATTCTTATCAGATTCTTCAAATCACGTCCTTTTTTTCTTCTTTAAATTTGTTACCATGGCAAATTCATCATCATTATTGTTGTTGTTGACGTTGTCACTTTTTGACAATGCAACTTTTCATTTCATTTTATGGTAGAGACAAGAAGGTATTGCTGAAGTATTGAATTGTTTTGTTTGTTTTAATGTTTATTAGGCGAGTTATGCTGCATTTCTTGACAATGTTGTCTTCCACGTGGAAGCTATAACGCCGGAGCAATTTAAGCTTGCAATTATGGAGGAAGATCTTATCTGA
Protein sequence

>MS.gene25193.t1

MAMLPPGAGFLPTDEILVGYYLRNKNSEQVQNFHGSDLIRELNLYEYDPIELSYTSFYYTIDNRRQFYCFTAKVGSERRHCRTGFWMKKGITRDINGGDDNVVLGQKSLFMFYLGNSVKDAVRTNWIMYEYALADKPQAPFVVCRVFDCPPAMDLGAEFGALSDVEEGDAAGDDSDVTRKLKGAGAGSSDGGGAAEQGDASHFTDFQGSLQASYAAFLDNVVFHVEAITPEQFKLAIMEEDLI