AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25203

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25203.t1 MTR_1g031930 95.033 302 15 0 1 302 60 361 0.0 565
MS.gene25203.t1 MTR_1g031930 94.915 236 12 0 67 302 65 300 2.73e-152 428
MS.gene25203.t1 MTR_6g069870 32.131 305 152 9 16 277 68 360 4.17e-26 106
MS.gene25203.t1 MTR_2g029260 54.054 111 44 1 109 219 6 109 9.06e-24 94.0
MS.gene25203.t1 MTR_6g069870 31.834 289 142 9 16 261 68 344 2.06e-22 95.9
MS.gene25203.t1 MTR_6g069870 33.333 204 105 7 16 214 68 245 2.45e-16 78.6
MS.gene25203.t1 MTR_2g042900 30.061 163 82 3 113 275 110 240 6.91e-14 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25203.t1 AT3G17860 37.698 252 123 9 41 274 64 299 2.89e-28 111
MS.gene25203.t1 AT3G17860 37.698 252 123 9 41 274 64 299 2.89e-28 111
MS.gene25203.t1 AT3G17860 37.698 252 123 9 41 274 84 319 4.56e-28 111
MS.gene25203.t1 AT3G17860 37.698 252 123 9 41 274 97 332 5.61e-28 111
MS.gene25203.t1 AT1G70700 34.375 256 121 10 39 293 58 267 7.57e-25 100
MS.gene25203.t1 AT1G70700 33.594 256 99 8 39 293 58 243 2.04e-24 99.4
MS.gene25203.t1 AT1G48500 37.245 196 97 5 81 274 118 289 7.99e-21 90.9
MS.gene25203.t1 AT1G70700 33.482 224 104 9 39 261 58 237 1.10e-19 87.0
MS.gene25203.t1 AT1G48500 37.158 183 89 5 81 261 88 246 2.47e-17 80.1
MS.gene25203.t1 AT1G48500 37.158 183 89 5 81 261 118 276 4.15e-17 80.1

Find 76 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTAGCAGTGATGAGTTTA+TGG 0.220743 1.3:-17141381 MS.gene25203:CDS
GATTTCTTGTTGAGGTTATA+TGG 0.291399 1.3:+17140329 None:intergenic
GAGCTAGATGCCTGAACTTC+AGG 0.316257 1.3:+17141527 None:intergenic
CCGATCGTTGGTACTGTTGC+TGG 0.321376 1.3:-17142163 MS.gene25203:CDS
CGTTGGTACTGTTGCTGGTT+TGG 0.366943 1.3:-17142158 MS.gene25203:CDS
TTATCTGTTTCTTCACATAC+TGG 0.372825 1.3:-17141425 MS.gene25203:CDS
TCGAGCCAATGATGCTTTGC+GGG 0.375285 1.3:+17141100 None:intergenic
GCTTATCTGCAGCTTCAAAC+AGG 0.376253 1.3:-17141556 MS.gene25203:CDS
GGATTTGCTTGATTGGAAAC+TGG 0.402551 1.3:+17142310 None:intergenic
ATGTTAGCACCCATTGAATT+CGG 0.406156 1.3:+17140290 None:intergenic
AAGGCTAAAATGATATCTTC+TGG 0.412380 1.3:-17142826 None:intergenic
AGAAAGTGGATTTGCTTGAT+TGG 0.416332 1.3:+17142303 None:intergenic
CAGGGCTCAACCTGAAGTTC+AGG 0.416821 1.3:-17141537 MS.gene25203:CDS
GTGGTTGCATTGACTACTTT+GGG 0.425771 1.3:+17141302 None:intergenic
ATGTCTACAGGTGGACTTGT+AGG 0.425980 1.3:+17141476 None:intergenic
GATATCTTCTGGTTTCATAC+AGG 0.429332 1.3:-17142815 MS.gene25203:intron
GCAGGCCCGCAAAGCATCAT+TGG 0.432249 1.3:-17141105 MS.gene25203:CDS
GGCACAAGCCATCATGTTGT+TGG 0.447624 1.3:-17141588 MS.gene25203:CDS
GGTGGTTGCATTGACTACTT+TGG 0.451731 1.3:+17141301 None:intergenic
AAATTCACATTGTGTTGTAC+AGG 0.451805 1.3:+17142364 None:intergenic
TGCACAAATGCCGAATTCAA+TGG 0.463346 1.3:-17140300 MS.gene25203:CDS
AGGACTTGAAATACCAGAGC+AGG 0.472355 1.3:+17141448 None:intergenic
CTGCTACTTGAGCCACTCCC+TGG 0.474711 1.3:+17141395 None:intergenic
TGTTATCCTTGGTTTGCTAC+CGG 0.482361 1.3:+17140239 None:intergenic
TCACATACTGGTCCACAGCC+AGG 0.488582 1.3:-17141413 MS.gene25203:CDS
CAAGCAGATGTGGTAGGAGT+AGG 0.492652 1.3:+17141338 None:intergenic
CTTGAGCCACTCCCTGGCTG+TGG 0.494035 1.3:+17141401 None:intergenic
GTATGTGAAGAAACAGATAA+AGG 0.494684 1.3:+17141428 None:intergenic
ATCGAGCCAATGATGCTTTG+CGG 0.499003 1.3:+17141099 None:intergenic
AATGCGAAGCAATCACTACT+TGG 0.504587 1.3:-17142220 MS.gene25203:CDS
GAGTTACTCACTGTTATCCT+TGG 0.504866 1.3:+17140228 None:intergenic
GCACAAATGCCGAATTCAAT+GGG 0.507087 1.3:-17140299 MS.gene25203:CDS
GCAACCACCATGGTTCCATC+AGG 0.516533 1.3:-17141287 MS.gene25203:intron
CATCCTCAGATTTCTTGTTG+AGG 0.517588 1.3:+17140321 None:intergenic
ACTGAATGAGGTGCTGTAAC+CGG 0.521363 1.3:+17142190 None:intergenic
AACATGGTAAGTTGAGGAGC+AGG 0.523147 1.3:+17142018 None:intergenic
AAATTCGCATCAGGTGATGA+TGG 0.528192 1.3:-17141503 MS.gene25203:CDS
GAGCAGGGTGGTATGTCTAC+AGG 0.528533 1.3:+17141464 None:intergenic
CATTCAGTTCTTCCGATCGT+TGG 0.534965 1.3:-17142175 MS.gene25203:CDS
TCTAGCTCGAAATTCGCATC+AGG 0.536374 1.3:-17141512 MS.gene25203:CDS
AGCGGATGTACCGCAGAAAG+TGG 0.538117 1.3:+17142289 None:intergenic
CACAACTTTGCAAGCAGATG+TGG 0.540084 1.3:+17141328 None:intergenic
ATCACTACTTGGTGGATCAC+CGG 0.541418 1.3:-17142209 MS.gene25203:CDS
CACTAGTACTGCTAATATGC+CGG 0.542872 1.3:-17140258 MS.gene25203:CDS
GTAGACATACCACCCTGCTC+TGG 0.550254 1.3:-17141461 MS.gene25203:CDS
CACAGTACCGCCATAAAACA+TGG 0.558159 1.3:+17142002 None:intergenic
ATCATGTTGTTGGCTAGAAG+TGG 0.558334 1.3:-17141578 MS.gene25203:CDS
AAGCAGATGTGGTAGGAGTA+GGG 0.560739 1.3:+17141339 None:intergenic
GCGAAGCAATCACTACTTGG+TGG 0.562781 1.3:-17142217 MS.gene25203:CDS
AATGATGCTTTGCGGGCCTG+CGG 0.566874 1.3:+17141107 None:intergenic
AGTTGAGGAGCAGGTGCAGA+TGG 0.568414 1.3:+17142027 None:intergenic
CAATGATATCACTCCTGAGA+AGG 0.574823 1.3:-17141971 MS.gene25203:intron
ACTGGTCCACAGCCAGGGAG+TGG 0.579269 1.3:-17141407 MS.gene25203:CDS
ATTAGCAGTACTAGTGGTCG+CGG 0.581937 1.3:+17140265 None:intergenic
CACATACTGGTCCACAGCCA+GGG 0.588678 1.3:-17141412 MS.gene25203:CDS
CTTATCTGCAGCTTCAAACA+GGG 0.597255 1.3:-17141555 MS.gene25203:CDS
ACTTCTAGCCAACAACATGA+TGG 0.599680 1.3:+17141580 None:intergenic
CGGCATATTAGCAGTACTAG+TGG 0.617445 1.3:+17140259 None:intergenic
TAGAACTCTTTCAAACACGA+TGG 0.630111 1.3:-17142421 MS.gene25203:CDS
TCTTTCAAACACGATGGACA+AGG 0.634289 1.3:-17142415 MS.gene25203:CDS
GGACTTGAAATACCAGAGCA+GGG 0.635730 1.3:+17141449 None:intergenic
CCAGCAACAGTACCAACGAT+CGG 0.636094 1.3:+17142163 None:intergenic
CCATAAAACATGGTAAGTTG+AGG 0.638856 1.3:+17142012 None:intergenic
ATGGCTGCTAAAACATCTAG+AGG 0.639805 1.3:-17141362 MS.gene25203:CDS
ACGATCGGAAGAACTGAATG+AGG 0.643920 1.3:+17142178 None:intergenic
CAGGGTGGTATGTCTACAGG+TGG 0.644694 1.3:+17141467 None:intergenic
TTGCTGGTTTGGTTGAACCA+TGG 0.654058 1.3:-17142147 MS.gene25203:intron
TAACCTCAACAAGAAATCTG+AGG 0.657118 1.3:-17140324 MS.gene25203:CDS
TCAAGCAAATCCACTTTCTG+CGG 0.659154 1.3:-17142299 MS.gene25203:CDS
TTACATACCTGATGGAACCA+TGG 0.681302 1.3:+17141280 None:intergenic
AATATGCCGGTAGCAAACCA+AGG 0.684244 1.3:-17140245 MS.gene25203:CDS
AGTAGTCAATGCAACCACCA+TGG 0.687562 1.3:-17141297 MS.gene25203:CDS
TTCAAACACGATGGACAAGG+TGG 0.707235 1.3:-17142412 MS.gene25203:CDS
ACTTTGCAAGCAGATGTGGT+AGG 0.708662 1.3:+17141332 None:intergenic
CTTGAAATACCAGAGCAGGG+TGG 0.775660 1.3:+17141452 None:intergenic
CATACCTGATGGAACCATGG+TGG 0.816498 1.3:+17141283 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATAAGATTTATTTTCA+CGG - chr1.3:17142541-17142560 MS.gene25203:intron 10.0%
!! CCAAAAGTTATAATAAATAT+AGG - chr1.3:17141852-17141871 MS.gene25203:intron 15.0%
!!! ATAAATCTTATAATTTGTGT+TGG + chr1.3:17142536-17142555 None:intergenic 15.0%
!!! ATTCATATGAAATTGATTTT+CGG - chr1.3:17140487-17140506 MS.gene25203:intron 15.0%
!!! CCTATATTTATTATAACTTT+TGG + chr1.3:17141855-17141874 None:intergenic 15.0%
!!! CTAGATGTATAAAATTATTT+TGG + chr1.3:17142657-17142676 None:intergenic 15.0%
!!! TTAATTATACAAGTCTTTTT+AGG - chr1.3:17140459-17140478 MS.gene25203:intron 15.0%
!! AAAACAAATTCACATTTAGA+GGG - chr1.3:17140371-17140390 MS.gene25203:intron 20.0%
!! AAACATCCTAAAATTATGTT+CGG + chr1.3:17140515-17140534 None:intergenic 20.0%
!! CCTAATAAATCTTTATAAGT+GGG - chr1.3:17142160-17142179 MS.gene25203:CDS 20.0%
!! TAAAACAAATTCACATTTAG+AGG - chr1.3:17140370-17140389 MS.gene25203:intron 20.0%
!! TGTTAAAAAATTGTGAAGTT+AGG - chr1.3:17140300-17140319 MS.gene25203:CDS 20.0%
!!! AAAAATCATCTTGTAACTTT+TGG - chr1.3:17140796-17140815 MS.gene25203:intron 20.0%
!!! AAGTTACAAGATGATTTTTT+AGG + chr1.3:17140795-17140814 None:intergenic 20.0%
!!! GATGTATAAAATTATTTTGG+AGG + chr1.3:17142654-17142673 None:intergenic 20.0%
!!! TGAAAAAGTGTAAATAAAGT+GGG - chr1.3:17142468-17142487 MS.gene25203:intron 20.0%
! AAAAACAGAAATTGAGAGAA+AGG + chr1.3:17140280-17140299 None:intergenic 25.0%
! AAATCAATCCAAGTAGAATA+GGG + chr1.3:17141135-17141154 None:intergenic 25.0%
! ATTCAAAAGTCTTTATAGGT+GGG - chr1.3:17142260-17142279 MS.gene25203:CDS 25.0%
! CATTGAACCAATTTATTTAG+AGG + chr1.3:17142602-17142621 None:intergenic 25.0%
! CCCACTTATAAAGATTTATT+AGG + chr1.3:17142163-17142182 None:intergenic 25.0%
! GCCTAATAAATCTTTATAAG+TGG - chr1.3:17142159-17142178 MS.gene25203:CDS 25.0%
! GTATGATTTCCTTTATACTT+GGG + chr1.3:17141380-17141399 None:intergenic 25.0%
! TAAATCAATCCAAGTAGAAT+AGG + chr1.3:17141136-17141155 None:intergenic 25.0%
! TAAATGGTAAGAACAAACAA+TGG + chr1.3:17140619-17140638 None:intergenic 25.0%
! TAATAACTCCATACTACAAA+AGG + chr1.3:17141884-17141903 None:intergenic 25.0%
! TGTATGATTTCCTTTATACT+TGG + chr1.3:17141381-17141400 None:intergenic 25.0%
! TTGTCATAAACTAACTCATT+AGG - chr1.3:17141257-17141276 MS.gene25203:intron 25.0%
!! AATAAATTGGTTCAATGTGT+TGG - chr1.3:17142605-17142624 MS.gene25203:intron 25.0%
!! ACCTATAAAGACTTTTGAAT+GGG + chr1.3:17142260-17142279 None:intergenic 25.0%
!! CTGAAAAAGTGTAAATAAAG+TGG - chr1.3:17142467-17142486 MS.gene25203:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+AGG - chr1.3:17141354-17141373 MS.gene25203:CDS 25.0%
!!! AAAGATGATAACTTGTTGTA+TGG + chr1.3:17142050-17142069 None:intergenic 25.0%
!!! CACTTTATTTACACTTTTTC+AGG + chr1.3:17142469-17142488 None:intergenic 25.0%
!!! TTTTGACTGAAGTTAAACAT+AGG - chr1.3:17141429-17141448 MS.gene25203:CDS 25.0%
AAAGGTTAGATCTAATACAC+GGG - chr1.3:17142000-17142019 MS.gene25203:CDS 30.0%
AAATTCACATTGTGTTGTAC+AGG + chr1.3:17140697-17140716 None:intergenic 30.0%
AATAACCTGAAAACAGCTTA+TGG + chr1.3:17141239-17141258 None:intergenic 30.0%
AATCAATCCAAGTAGAATAG+GGG + chr1.3:17141134-17141153 None:intergenic 30.0%
AGAGTCTATCCAAAATTTGT+TGG - chr1.3:17142357-17142376 MS.gene25203:CDS 30.0%
AGGTTTGATCAATTGTATTC+AGG - chr1.3:17141449-17141468 MS.gene25203:CDS 30.0%
ATATATTCCCCTATTCTACT+TGG - chr1.3:17141124-17141143 MS.gene25203:intron 30.0%
CATTCAAAAGTCTTTATAGG+TGG - chr1.3:17142259-17142278 MS.gene25203:CDS 30.0%
GAAAGGTTAGATCTAATACA+CGG - chr1.3:17141999-17142018 MS.gene25203:CDS 30.0%
GACCATCTTATAATTTGAAG+TGG + chr1.3:17142231-17142250 None:intergenic 30.0%
GATTTCTTGTTGAGGTTATA+TGG + chr1.3:17142732-17142751 None:intergenic 30.0%
GGTCAACACAAATTATAAGA+TGG - chr1.3:17142326-17142345 MS.gene25203:CDS 30.0%
GTATGTGAAGAAACAGATAA+AGG + chr1.3:17141633-17141652 None:intergenic 30.0%
TCGTTTGCCTCTAAATAAAT+TGG - chr1.3:17142592-17142611 MS.gene25203:intron 30.0%
TTATACTGTTACATACCTGA+TGG + chr1.3:17141789-17141808 None:intergenic 30.0%
! CACCTATAAAGACTTTTGAA+TGG + chr1.3:17142261-17142280 None:intergenic 30.0%
! GAGTCTATCCAAAATTTGTT+GGG - chr1.3:17142358-17142377 MS.gene25203:CDS 30.0%
! TTATCTGTTTCTTCACATAC+TGG - chr1.3:17141633-17141652 MS.gene25203:intron 30.0%
!! GATAACTTGTTGTATGGAAA+AGG + chr1.3:17142044-17142063 None:intergenic 30.0%
!! GTGTTTGAAAGAGTTCTAAA+TGG + chr1.3:17140635-17140654 None:intergenic 30.0%
!!! ACAAGATGATTTTTTAGGAG+CGG + chr1.3:17140790-17140809 None:intergenic 30.0%
AACAAACAATGGAAGGAAGT+GGG + chr1.3:17140608-17140627 None:intergenic 35.0%
AAGTAGCAGTGATGAGTTTA+TGG - chr1.3:17141677-17141696 MS.gene25203:intron 35.0%
AGGACAACATTCAGTTAATC+AGG - chr1.3:17140320-17140339 MS.gene25203:CDS 35.0%
AGGCTATATCACATCTGATT+TGG + chr1.3:17142449-17142468 None:intergenic 35.0%
ATATAACTCACCTTACAAGC+CGG - chr1.3:17142185-17142204 MS.gene25203:CDS 35.0%
ATGTTAGCACCCATTGAATT+CGG + chr1.3:17142771-17142790 None:intergenic 35.0%
CAATGCTAGAGATGTTTCTT+AGG - chr1.3:17140945-17140964 MS.gene25203:intron 35.0%
CCATAAAACATGGTAAGTTG+AGG + chr1.3:17141049-17141068 None:intergenic 35.0%
CTAGAGTGTGATCCAATAAA+GGG + chr1.3:17142387-17142406 None:intergenic 35.0%
CTAGTTCAACCTTACAAAAC+CGG + chr1.3:17142207-17142226 None:intergenic 35.0%
GCATTGAACTGAAATTACCA+TGG + chr1.3:17140931-17140950 None:intergenic 35.0%
GCCCATTCAAAAGTCTTTAT+AGG - chr1.3:17142256-17142275 MS.gene25203:CDS 35.0%
GGCCACTTCAAATTATAAGA+TGG - chr1.3:17142226-17142245 MS.gene25203:CDS 35.0%
GGCTATATCACATCTGATTT+GGG + chr1.3:17142448-17142467 None:intergenic 35.0%
TAACCTCAACAAGAAATCTG+AGG - chr1.3:17142734-17142753 MS.gene25203:intron 35.0%
TAGAACTCTTTCAAACACGA+TGG - chr1.3:17140637-17140656 MS.gene25203:intron 35.0%
TGAGAGAAAAATACCTTCTC+AGG + chr1.3:17141103-17141122 None:intergenic 35.0%
TGGTAAGAACAAACAATGGA+AGG + chr1.3:17140615-17140634 None:intergenic 35.0%
! AATGAGAGTATGAGACTGTT+TGG - chr1.3:17141184-17141203 MS.gene25203:intron 35.0%
! AATTGGTTCAATGTGTTGGA+AGG - chr1.3:17142609-17142628 MS.gene25203:intron 35.0%
! AGAAAGTGGATTTGCTTGAT+TGG + chr1.3:17140758-17140777 None:intergenic 35.0%
! CAACTTACCATGTTTTATGG+CGG - chr1.3:17141049-17141068 MS.gene25203:intron 35.0%
! CATGTGTTTCTGTCAATACA+GGG - chr1.3:17142692-17142711 MS.gene25203:intron 35.0%
! CCTCAACTTACCATGTTTTA+TGG - chr1.3:17141046-17141065 MS.gene25203:intron 35.0%
! GAGATGTTTCTTAGGAGTTT+TGG - chr1.3:17140953-17140972 MS.gene25203:intron 35.0%
! TCATGTGTTTCTGTCAATAC+AGG - chr1.3:17142691-17142710 MS.gene25203:intron 35.0%
! TCGGATCCGAACATAATTTT+AGG - chr1.3:17140506-17140525 MS.gene25203:intron 35.0%
! TCTAGAGTGTGATCCAATAA+AGG + chr1.3:17142388-17142407 None:intergenic 35.0%
! TTTTCAGTGCAATCTGCTTA+CGG - chr1.3:17140430-17140449 MS.gene25203:intron 35.0%
!! GATATCTTCTGGTTTCATAC+AGG - chr1.3:17140243-17140262 MS.gene25203:CDS 35.0%
AAATTCGCATCAGGTGATGA+TGG - chr1.3:17141555-17141574 MS.gene25203:CDS 40.0%
AATGCGAAGCAATCACTACT+TGG - chr1.3:17140838-17140857 MS.gene25203:intron 40.0%
ACTTCTAGCCAACAACATGA+TGG + chr1.3:17141481-17141500 None:intergenic 40.0%
ATGGCTGCTAAAACATCTAG+AGG - chr1.3:17141696-17141715 MS.gene25203:intron 40.0%
ATTCACATTTAGAGGGTCGT+TGG - chr1.3:17140378-17140397 MS.gene25203:intron 40.0%
CAAAAGTCTTTATAGGTGGG+AGG - chr1.3:17142263-17142282 MS.gene25203:CDS 40.0%
CAAACAAGGCCCAAGTATAA+AGG - chr1.3:17141368-17141387 MS.gene25203:CDS 40.0%
CAATGATATCACTCCTGAGA+AGG - chr1.3:17141087-17141106 MS.gene25203:CDS 40.0%
CACTAGTACTGCTAATATGC+CGG - chr1.3:17142800-17142819 MS.gene25203:intron 40.0%
CACTCTAGATGTCAAGTTCA+TGG - chr1.3:17142399-17142418 MS.gene25203:CDS 40.0%
CATCCTCAGATTTCTTGTTG+AGG + chr1.3:17142740-17142759 None:intergenic 40.0%
CTTATCTGCAGCTTCAAACA+GGG - chr1.3:17141503-17141522 MS.gene25203:CDS 40.0%
GAACAAACAATGGAAGGAAG+TGG + chr1.3:17140609-17140628 None:intergenic 40.0%
GCACAAATGCCGAATTCAAT+GGG - chr1.3:17142759-17142778 MS.gene25203:intron 40.0%
GCATACATCACCTTACAAGT+CGG - chr1.3:17142285-17142304 MS.gene25203:CDS 40.0%
TCAAGCAAATCCACTTTCTG+CGG - chr1.3:17140759-17140778 MS.gene25203:intron 40.0%
TCTTTCAAACACGATGGACA+AGG - chr1.3:17140643-17140662 MS.gene25203:intron 40.0%
TGCACAAATGCCGAATTCAA+TGG - chr1.3:17142758-17142777 MS.gene25203:intron 40.0%
TTACATACCTGATGGAACCA+TGG + chr1.3:17141781-17141800 None:intergenic 40.0%
TTCAATGTGTTGGAAGGAAG+AGG - chr1.3:17142615-17142634 MS.gene25203:intron 40.0%
TTTGGAGAGCTTATCGAATC+AGG - chr1.3:17141202-17141221 MS.gene25203:intron 40.0%
! ATCATGTTGTTGGCTAGAAG+TGG - chr1.3:17141480-17141499 MS.gene25203:CDS 40.0%
! ATTTTATGCAGCTATACCGC+AGG - chr1.3:17141935-17141954 MS.gene25203:intron 40.0%
! GGATGCATCCTTTTGTAGTA+TGG - chr1.3:17141873-17141892 MS.gene25203:intron 40.0%
! GGATTTGCTTGATTGGAAAC+TGG + chr1.3:17140751-17140770 None:intergenic 40.0%
!! CATGTCCATAAGCTGTTTTC+AGG - chr1.3:17141231-17141250 MS.gene25203:intron 40.0%
!! CGGTTTTGTAAGGTTGAACT+AGG - chr1.3:17142205-17142224 MS.gene25203:CDS 40.0%
!! CTTTATACTTGGGCCTTGTT+TGG + chr1.3:17141370-17141389 None:intergenic 40.0%
!! GTGGTTGCATTGACTACTTT+GGG + chr1.3:17141759-17141778 None:intergenic 40.0%
!!! AGCATCATTGGCTCGATTTT+TGG - chr1.3:17141965-17141984 MS.gene25203:intron 40.0%
AAGCAGATGTGGTAGGAGTA+GGG + chr1.3:17141722-17141741 None:intergenic 45.0%
AATATGCCGGTAGCAAACCA+AGG - chr1.3:17142813-17142832 MS.gene25203:intron 45.0%
ACGATCGGAAGAACTGAATG+AGG + chr1.3:17140883-17140902 None:intergenic 45.0%
ACTTTGCAAGCAGATGTGGT+AGG + chr1.3:17141729-17141748 None:intergenic 45.0%
AGGACTTGAAATACCAGAGC+AGG + chr1.3:17141613-17141632 None:intergenic 45.0%
AGTAGTCAATGCAACCACCA+TGG - chr1.3:17141761-17141780 MS.gene25203:intron 45.0%
ATCGAGCCAATGATGCTTTG+CGG + chr1.3:17141962-17141981 None:intergenic 45.0%
ATTAGCAGTACTAGTGGTCG+CGG + chr1.3:17142796-17142815 None:intergenic 45.0%
CACAACTTTGCAAGCAGATG+TGG + chr1.3:17141733-17141752 None:intergenic 45.0%
CACAGTACCGCCATAAAACA+TGG + chr1.3:17141059-17141078 None:intergenic 45.0%
CATTCAGTTCTTCCGATCGT+TGG - chr1.3:17140883-17140902 MS.gene25203:intron 45.0%
CGGCATATTAGCAGTACTAG+TGG + chr1.3:17142802-17142821 None:intergenic 45.0%
GCTTATCTGCAGCTTCAAAC+AGG - chr1.3:17141502-17141521 MS.gene25203:CDS 45.0%
GGACTTGAAATACCAGAGCA+GGG + chr1.3:17141612-17141631 None:intergenic 45.0%
TCAAGTTCATGGTGTGACGA+GGG - chr1.3:17142410-17142429 MS.gene25203:CDS 45.0%
TTCAAACACGATGGACAAGG+TGG - chr1.3:17140646-17140665 MS.gene25203:intron 45.0%
TTTGTTGGGCGACCCTTTAT+TGG - chr1.3:17142372-17142391 MS.gene25203:CDS 45.0%
! AACATGGTAAGTTGAGGAGC+AGG + chr1.3:17141043-17141062 None:intergenic 45.0%
! AAGGGTCGCCCAACAAATTT+TGG + chr1.3:17142369-17142388 None:intergenic 45.0%
! ACCTTACAAGTCGGCTTTGT+AGG - chr1.3:17142294-17142313 MS.gene25203:CDS 45.0%
! ATCACTACTTGGTGGATCAC+CGG - chr1.3:17140849-17140868 MS.gene25203:intron 45.0%
! ATGTCTACAGGTGGACTTGT+AGG + chr1.3:17141585-17141604 None:intergenic 45.0%
! CCTTACAAGTCGGCTTTGTA+GGG - chr1.3:17142295-17142314 MS.gene25203:CDS 45.0%
! TCTAGCTCGAAATTCGCATC+AGG - chr1.3:17141546-17141565 MS.gene25203:CDS 45.0%
!! ACTGAATGAGGTGCTGTAAC+CGG + chr1.3:17140871-17140890 None:intergenic 45.0%
!! CCTTACAAAACCGGCTTGTA+AGG + chr1.3:17142198-17142217 None:intergenic 45.0%
!! CCTTACAAGCCGGTTTTGTA+AGG - chr1.3:17142195-17142214 MS.gene25203:CDS 45.0%
!! GGTGGTTGCATTGACTACTT+TGG + chr1.3:17141760-17141779 None:intergenic 45.0%
!! TCGATTTTTGGAGAAGCGCA+AGG - chr1.3:17141977-17141996 MS.gene25203:CDS 45.0%
!! TTGCTGGTTTGGTTGAACCA+TGG - chr1.3:17140911-17140930 MS.gene25203:intron 45.0%
!! TTTTGGAGAAGCGCAAGGAA+AGG - chr1.3:17141982-17142001 MS.gene25203:CDS 45.0%
CAAGCAGATGTGGTAGGAGT+AGG + chr1.3:17141723-17141742 None:intergenic 50.0%
CATACCTGATGGAACCATGG+TGG + chr1.3:17141778-17141797 None:intergenic 50.0%
CCAACTCAACCGCACAAAAC+CGG + chr1.3:17142512-17142531 None:intergenic 50.0%
CCAGCAACAGTACCAACGAT+CGG + chr1.3:17140898-17140917 None:intergenic 50.0%
CTTGAAATACCAGAGCAGGG+TGG + chr1.3:17141609-17141628 None:intergenic 50.0%
GAGCTAGATGCCTGAACTTC+AGG + chr1.3:17141534-17141553 None:intergenic 50.0%
GAGTCACACACCTTACAAGC+CGG - chr1.3:17142490-17142509 MS.gene25203:intron 50.0%
GCGAAGCAATCACTACTTGG+TGG - chr1.3:17140841-17140860 MS.gene25203:intron 50.0%
GGCACAAGCCATCATGTTGT+TGG - chr1.3:17141470-17141489 MS.gene25203:CDS 50.0%
GTCAAGTTCATGGTGTGACG+AGG - chr1.3:17142409-17142428 MS.gene25203:CDS 50.0%
TCGAGCCAATGATGCTTTGC+GGG + chr1.3:17141961-17141980 None:intergenic 50.0%
! CGGCTTTGTAGGGTTGAGTT+AGG - chr1.3:17142305-17142324 MS.gene25203:CDS 50.0%
! CGTTGGTACTGTTGCTGGTT+TGG - chr1.3:17140900-17140919 MS.gene25203:intron 50.0%
!! CCCTACAAAGCCGACTTGTA+AGG + chr1.3:17142298-17142317 None:intergenic 50.0%
!! CCTTACAAGCCGGTTTTGTG+CGG - chr1.3:17142500-17142519 MS.gene25203:intron 50.0%
!! CGGTTTTGTGCGGTTGAGTT+GGG - chr1.3:17142510-17142529 MS.gene25203:intron 50.0%
AATGATGCTTTGCGGGCCTG+CGG + chr1.3:17141954-17141973 None:intergenic 55.0%
AGCGGATGTACCGCAGAAAG+TGG + chr1.3:17140772-17140791 None:intergenic 55.0%
AGTTGAGGAGCAGGTGCAGA+TGG + chr1.3:17141034-17141053 None:intergenic 55.0%
CACATACTGGTCCACAGCCA+GGG - chr1.3:17141646-17141665 MS.gene25203:intron 55.0%
CAGGGCTCAACCTGAAGTTC+AGG - chr1.3:17141521-17141540 MS.gene25203:CDS 55.0%
CAGGGTGGTATGTCTACAGG+TGG + chr1.3:17141594-17141613 None:intergenic 55.0%
CCGATCGTTGGTACTGTTGC+TGG - chr1.3:17140895-17140914 MS.gene25203:intron 55.0%
GAGCAGGGTGGTATGTCTAC+AGG + chr1.3:17141597-17141616 None:intergenic 55.0%
GCAACCACCATGGTTCCATC+AGG - chr1.3:17141771-17141790 MS.gene25203:intron 55.0%
GTAGACATACCACCCTGCTC+TGG - chr1.3:17141597-17141616 MS.gene25203:intron 55.0%
TCACATACTGGTCCACAGCC+AGG - chr1.3:17141645-17141664 MS.gene25203:intron 55.0%
!! CCGCACAAAACCGGCTTGTA+AGG + chr1.3:17142503-17142522 None:intergenic 55.0%
!! CCGGTTTTGTGCGGTTGAGT+TGG - chr1.3:17142509-17142528 MS.gene25203:intron 55.0%
GGACTCGAGCGTGAGCAGAT+AGG - chr1.3:17140399-17140418 MS.gene25203:intron 60.0%
! CTGCTACTTGAGCCACTCCC+TGG + chr1.3:17141666-17141685 None:intergenic 60.0%
! GCAGGCCCGCAAAGCATCAT+TGG - chr1.3:17141953-17141972 MS.gene25203:intron 60.0%
ACTGGTCCACAGCCAGGGAG+TGG - chr1.3:17141651-17141670 MS.gene25203:intron 65.0%
CTTGAGCCACTCCCTGGCTG+TGG + chr1.3:17141660-17141679 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 17140241 17142839 17140241 ID=MS.gene25203
chr1.3 mRNA 17140241 17142839 17140241 ID=MS.gene25203.t1;Parent=MS.gene25203
chr1.3 exon 17142816 17142839 17142816 ID=MS.gene25203.t1.exon1;Parent=MS.gene25203.t1
chr1.3 CDS 17142816 17142839 17142816 ID=cds.MS.gene25203.t1;Parent=MS.gene25203.t1
chr1.3 exon 17142148 17142440 17142148 ID=MS.gene25203.t1.exon2;Parent=MS.gene25203.t1
chr1.3 CDS 17142148 17142440 17142148 ID=cds.MS.gene25203.t1;Parent=MS.gene25203.t1
chr1.3 exon 17141972 17142056 17141972 ID=MS.gene25203.t1.exon3;Parent=MS.gene25203.t1
chr1.3 CDS 17141972 17142056 17141972 ID=cds.MS.gene25203.t1;Parent=MS.gene25203.t1
chr1.3 exon 17141288 17141609 17141288 ID=MS.gene25203.t1.exon4;Parent=MS.gene25203.t1
chr1.3 CDS 17141288 17141609 17141288 ID=cds.MS.gene25203.t1;Parent=MS.gene25203.t1
chr1.3 exon 17141077 17141134 17141077 ID=MS.gene25203.t1.exon5;Parent=MS.gene25203.t1
chr1.3 CDS 17141077 17141134 17141077 ID=cds.MS.gene25203.t1;Parent=MS.gene25203.t1
chr1.3 exon 17140241 17140367 17140241 ID=MS.gene25203.t1.exon6;Parent=MS.gene25203.t1
chr1.3 CDS 17140241 17140367 17140241 ID=cds.MS.gene25203.t1;Parent=MS.gene25203.t1
Gene Sequence

>MS.gene25203

ATGATATCTTCTGGTTTCATACAGGTTTGATAATATCCTTTCTCTCAATTTCTGTTTTTTGTTAAAAAATTGTGAAGTTAGGACAACATTCAGTTAATCAGGATTTCAAATTCATCAAATTTAAGTGATTAAAACAAATTCACATTTAGAGGGTCGTTGGACTCGAGCGTGAGCAGATAGGCATTTTAATTTTCAGTGCAATCTGCTTACGGTATCTATTAATTATACAAGTCTTTTTAGGATATGATTCATATGAAATTGATTTTCGGATCCGAACATAATTTTAGGATGTTTTGCAACATATCATGTCTGTTGTTCTCATACATTTGATCACACATTTAATAAGTATAATCTGACGTCTTTTCCCACTTCCTTCCATTGTTTGTTCTTACCATTTAGAACTCTTTCAAACACGATGGACAAGGTGGTATTCATTTTTCTGTGAATCAGTATCCTGTACAACACAATGTGAATTTTATGAATCGTCATCATGATGTGAAGATGTTTCCAGTTTCCAATCAAGCAAATCCACTTTCTGCGGTACATCCGCTCCTAAAAAATCATCTTGTAACTTTTGGTCAGAATATGAACGTTGCTAATGCGAAGCAATCACTACTTGGTGGATCACCGGTTACAGCACCTCATTCAGTTCTTCCGATCGTTGGTACTGTTGCTGGTTTGGTTGAACCATGGTAATTTCAGTTCAATGCTAGAGATGTTTCTTAGGAGTTTTGGTTTGAATTCAACTAACTTGTTTGTCTCATTTATATTATGCTTCAACAGTGAGATACCATCTGCACCTGCTCCTCAACTTACCATGTTTTATGGCGGTACTGTGAACATCTTCAATGATATCACTCCTGAGAAGGTATTTTTCTCTCATATATATTCCCCTATTCTACTTGGATTGATTTATTTGAGCTTGTCTACTATCATAAGCACTAATGAGAGTATGAGACTGTTTGGAGAGCTTATCGAATCAGGTTATGACATGTCCATAAGCTGTTTTCAGGTTATTGTCATAAACTAACTCATTAGGATAGCTTATGAAAACAGCTTATAGCTTCAATAAAAACAATATGACTATTTTATTTTATTTTACTGTAAAGTAAATTAAGTTGTTTATCCAAACAAGGCCCAAGTATAAAGGAAATCATACATCTCAGAGTTAAGCTTAAGTATGTTTTATTTTGACTGAAGTTAAACATAGGTTTGATCAATTGTATTCAGGCACAAGCCATCATGTTGTTGGCTAGAAGTGGCTTATCTGCAGCTTCAAACAGGGCTCAACCTGAAGTTCAGGCATCTAGCTCGAAATTCGCATCAGGTGATGATGGTTTGCCTACAAGTCCACCTGTAGACATACCACCCTGCTCTGGTATTTCAAGTCCTTTATCTGTTTCTTCACATACTGGTCCACAGCCAGGGAGTGGCTCAAGTAGCAGTGATGAGTTTATGGCTGCTAAAACATCTAGAGGCCCTACTCCTACCACATCTGCTTGCAAAGTTGTGACTCCCAAAGTAGTCAATGCAACCACCATGGTTCCATCAGGTATGTAACAGTATAATTTCTTGCTACATGATGTTATTTTACTTATTCATATTCTTTTGCCAAAAGTTATAATAAATATAGGATGCATCCTTTTGTAGTATGGAGTTATTAATCTGTTTGATCATTTTTACTGTCATATAATATTTTATGCAGCTATACCGCAGGCCCGCAAAGCATCATTGGCTCGATTTTTGGAGAAGCGCAAGGAAAGGTTAGATCTAATACACGGGTTCTATTTCTCATCATTACCTTTTCCATACAACAAGTTATCATCTTTAAATTGTTGAAACTTGAAACATGTTGATTTCTTTTTCTTTATGAGTTTTTCATTGTTGAGAGTTTTACTTTTACATCAGATGTGATGTAGCCTAATAAATCTTTATAAGTGGGAGATATAACTCACCTTACAAGCCGGTTTTGTAAGGTTGAACTAGGCCACTTCAAATTATAAGATGGTCTCAGAGCCCATTCAAAAGTCTTTATAGGTGGGAGGCATACATCACCTTACAAGTCGGCTTTGTAGGGTTGAGTTAGGTCAACACAAATTATAAGATGGTCTGTCTGAGAGTCTATCCAAAATTTGTTGGGCGACCCTTTATTGGATCACACTCTAGATGTCAAGTTCATGGTGTGACGAGGGTCTGTTAAGCGTCCCAAATCAGATGTGATATAGCCTGAAAAAGTGTAAATAAAGTGGGAGTCACACACCTTACAAGCCGGTTTTGTGCGGTTGAGTTGGGCCAACACAAATTATAAGATTTATTTTCACGGTTTTAAACTTTTATATTGTCTATACTGCTCGTTTGCCTCTAAATAAATTGGTTCAATGTGTTGGAAGGAAGAGGAAGAAGTAACTCACCTCCAAAATAATTTTATACATCTAGTTTAGTTAAGATTCTCATGTGTTTCTGTCAATACAGGGTGATGAGTGCAGCACCATATAACCTCAACAAGAAATCTGAGGATGCACAAATGCCGAATTCAATGGGTGCTAACATCTCCGCGACCACTAGTACTGCTAATATGCCGGTAGCAAACCAAGGATAA
Protein sequence

>MS.gene25203.t1

MISSGFIQNSFKHDGQGGIHFSVNQYPVQHNVNFMNRHHDVKMFPVSNQANPLSAVHPLLKNHLVTFGQNMNVANAKQSLLGGSPVTAPHSVLPIVGTVAGLVEPCEIPSAPAPQLTMFYGGTVNIFNDITPEKAQAIMLLARSGLSAASNRAQPEVQASSSKFASGDDGLPTSPPVDIPPCSGISSPLSVSSHTGPQPGSGSSSSDEFMAAKTSRGPTPTTSACKVVTPKVVNATTMVPSAIPQARKASLARFLEKRKERVMSAAPYNLNKKSEDAQMPNSMGANISATTSTANMPVANQG