AlfalfaGEDB | MS.gene25251

M. sativa cultivar XinJiangDaYe / MS.gene25251

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25251.t1	XP_024639353.1	85.5	131	19	0	98	228	1	131	2.00E-61	245.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25251.t1	G7JX45	88.2	85	10	0	144	228	25	109	2.9e-38	168.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene25251.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25251.t1	MTR_5g053510	88.235	85	10	0	144	228	25	109	1.18e-50	160
MS.gene25251.t1	MTR_4g123470	34.314	204	124	5	11	211	14	210	2.67e-30	112
MS.gene25251.t1	MTR_1g054495	27.876	226	156	6	1	225	1	220	7.03e-25	98.2
MS.gene25251.t1	MTR_0014s0220	30.631	222	131	7	5	225	623	822	3.16e-23	97.8
MS.gene25251.t1	MTR_4g053550	52.113	71	33	1	24	93	47	117	1.51e-19	81.3
MS.gene25251.t1	MTR_4g085790	28.000	225	155	5	1	224	1	219	1.53e-19	84.0
MS.gene25251.t1	MTR_7g025150	28.000	225	155	5	1	224	1	219	4.87e-19	82.8
MS.gene25251.t1	MTR_1g041870	32.778	180	104	5	49	228	13	175	1.36e-16	75.1
MS.gene25251.t1	MTR_1g054485	31.343	134	89	3	1	133	1	132	1.15e-14	70.1
MS.gene25251.t1	MTR_7g039210	21.212	231	152	5	1	203	1	229	7.04e-12	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 40 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAAAATGATTTGCGGTT+CGG	0.410181	8.2:-7402727	None:intergenic
AAATCGTGCTAAACTATGTT+GGG	0.473587	8.2:+7402620	MS.gene25251:CDS
AACAACAATGCATTCCAGAT+TGG	0.510902	8.2:+7403056	MS.gene25251:CDS
AACGAATAGTGTCTCCAATC+TGG	0.387174	8.2:-7403070	None:intergenic
AACTGTACATCTTCCAGATC+AGG	0.417936	8.2:+7402290	MS.gene25251:CDS
AAGAAATCATTGAAGTCTGG+TGG	0.620565	8.2:-7402900	None:intergenic
AATATATACAAAACCTGATC+TGG	0.390568	8.2:-7402303	None:intergenic
AATGTAACTTTCAACAATTC+AGG	0.262321	8.2:+7402529	MS.gene25251:CDS
ACATCTATCAGATTCCAAAC+TGG	0.476477	8.2:-7402490	None:intergenic
AGACTTCAATGATTTCTTAT+GGG	0.387413	8.2:+7402905	MS.gene25251:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCGTGCTAAACTATGTT+GGG	+	chr8.2:7402620-7402639	MS.gene25251:CDS	30.0%
ACATAGTTTGGTTAGAAAAC+AGG	-	chr8.2:7402831-7402850	None:intergenic	30.0%
ATCAGATAAAAAACAGCAGT+TGG	-	chr8.2:7402337-7402356	None:intergenic	30.0%
CAGACTTCAATGATTTCTTA+TGG	+	chr8.2:7402904-7402923	MS.gene25251:CDS	30.0%
CATAAGAAATCATTGAAGTC+TGG	-	chr8.2:7402906-7402925	None:intergenic	30.0%
TGGAATCTGATAGATGTTAA+TGG	+	chr8.2:7402496-7402515	MS.gene25251:CDS	30.0%
TGGTGAAAAAACACATAGTT+TGG	-	chr8.2:7402843-7402862	None:intergenic	30.0%
TTCCAAGAGTTAGTATGAAT+CGG	-	chr8.2:7402651-7402670	None:intergenic	30.0%
TTCCTATAGACATGAACAAA+AGG	-	chr8.2:7403122-7403141	None:intergenic	30.0%
AACAACAATGCATTCCAGAT+TGG	+	chr8.2:7403056-7403075	MS.gene25251:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	7402240	7403151	7402240	ID=MS.gene25251
chr8.2	mRNA	7402240	7403151	7402240	ID=MS.gene25251.t1;Parent=MS.gene25251
chr8.2	exon	7402240	7402311	7402240	ID=MS.gene25251.t1.exon1;Parent=MS.gene25251.t1
chr8.2	CDS	7402240	7402311	7402240	ID=cds.MS.gene25251.t1;Parent=MS.gene25251.t1
chr8.2	exon	7402439	7402795	7402439	ID=MS.gene25251.t1.exon2;Parent=MS.gene25251.t1
chr8.2	CDS	7402439	7402795	7402439	ID=cds.MS.gene25251.t1;Parent=MS.gene25251.t1
chr8.2	exon	7402894	7403151	7402894	ID=MS.gene25251.t1.exon3;Parent=MS.gene25251.t1
chr8.2	CDS	7402894	7403151	7402894	ID=cds.MS.gene25251.t1;Parent=MS.gene25251.t1

Gene Sequence

>MS.gene25251

ATGGCTGGATATCGTGCTGTACTCAAACGACAAGTTTTGGGAGGTTTTGAAACTGTACATCTTCCAGATCAGGTTTTGTATATATTCATCTTTACCAACTGCTGTTTTTTATCTGATACAATACTATACCCTGTTTGTAAACTTAATAATATTATCGATTGCATTCATCTTCGTTTATAAATGTTGATTATGTGTACAGGAATTCGCCGAAGTGAACCCAAAATTTCTTGAAGAATTCGATGATTTGAAACCAGTTTGGAATCTGATAGATGTTAATGGAAACTATCATAATGTAACTTTCAACAATTCAGGAATCCTCCCTTTACTCACTACAGGATGGACTGACATCAGAGATTTTTACCATTTCGAAGGCATCAGACAAATCGTGCTAAACTATGTTGGGCAAAGCCGATTCATACTAACTCTTGGAAGGACGCTTCAGACCACTGATGAATTCCCTCCCTTCCACAGCAGAAGCACAAAACTACCGAACCGCAAATCATTTTATTTTGACATCACTTTGACTCATTTGCAGATGGCAGAAAGATCTGAATTGGTATAATTTAATTCTAATCACATATATTTGATCCTGTTTTCTAACCAAACTATGTGTTTTTTCACCAATTTAACAAAAACCAATTTTGCAAATTACAGGTTCTGCCACCAGACTTCAATGATTTCTTATGGGATAGTGATTTCGATTACCTTTTTCTCTGCAATGCAAATCCGAATGAAGAGTTTGTTACAGCTGAAATTGAATACCAAAGCAATGAATCGCTGAATACTAAGTTAAAAAATGGTTGGGAGACATTCTGTAACAACAATGCATTCCAGATTGGAGACACTATTCGTTTCAAGTTCTTCGATGGGAAAAACACTCCTTTTGTTCATGTCTATAGGAACAATCCTTAA

Protein sequence

>MS.gene25251.t1

MAGYRAVLKRQVLGGFETVHLPDQEFAEVNPKFLEEFDDLKPVWNLIDVNGNYHNVTFNNSGILPLLTTGWTDIRDFYHFEGIRQIVLNYVGQSRFILTLGRTLQTTDEFPPFHSRSTKLPNRKSFYFDITLTHLQMAERSELVLPPDFNDFLWDSDFDYLFLCNANPNEEFVTAEIEYQSNESLNTKLKNGWETFCNNNAFQIGDTIRFKFFDGKNTPFVHVYRNNP