Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25301.t1 | XP_003628592.1 | 91.7 | 181 | 15 | 0 | 1 | 181 | 141 | 321 | 5.80E-96 | 360.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25301.t1 | Q338B9 | 74.3 | 183 | 41 | 4 | 1 | 181 | 208 | 386 | 1.5e-76 | 287.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25301.t1 | G7LEM3 | 91.7 | 181 | 15 | 0 | 1 | 181 | 141 | 321 | 4.2e-96 | 360.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25301.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25301.t1 | MTR_8g062330 | 91.713 | 181 | 15 | 0 | 1 | 181 | 141 | 321 | 9.28e-125 | 358 |
| MS.gene25301.t1 | MTR_7g096040 | 76.374 | 182 | 38 | 4 | 1 | 181 | 239 | 416 | 2.28e-97 | 292 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25301.t1 | AT3G54610 | 71.585 | 183 | 46 | 4 | 1 | 181 | 264 | 442 | 6.08e-92 | 279 |
Find 40 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAGTCTATAATGGTTATA+AGG | 0.249320 | 8.2:+38247090 | None:intergenic |
| AGTGATGATCAACAAGTAAA+AGG | 0.317455 | 8.2:+38247194 | MS.gene25301:CDS |
| CGCGAATGACCGTACATATC+AGG | 0.322015 | 8.2:-38247617 | None:intergenic |
| ATGTCATCAACAATTCTATT+AGG | 0.362385 | 8.2:-38247566 | None:intergenic |
| TATGCTGATAATAATGCTAT+TGG | 0.385429 | 8.2:+38247290 | MS.gene25301:CDS |
| ATAGTTGATAAATCAGTATA+AGG | 0.397900 | 8.2:-38247431 | None:intergenic |
| CTCTTCTGAACATCAATTCC+AGG | 0.397903 | 8.2:-38247524 | None:intergenic |
| GCGAACCTTTGATTTGCATA+TGG | 0.399002 | 8.2:-38247149 | None:intergenic |
| TAAGTCTATAATGGTTATAA+GGG | 0.403247 | 8.2:+38247091 | None:intergenic |
| TAGGAACCATAAGTCTATAA+TGG | 0.415215 | 8.2:+38247082 | None:intergenic |
| GATCAACAAGTAAAAGGTTA+TGG | 0.418177 | 8.2:+38247200 | MS.gene25301:CDS |
| AAATCAGTATAAGGAAGCTT+TGG | 0.423435 | 8.2:-38247422 | None:intergenic |
| TGTCACAATATTGTTTATCC+TGG | 0.433111 | 8.2:+38247506 | MS.gene25301:CDS |
| TCGATGGGAAGGTTATATAA+AGG | 0.445441 | 8.2:+38247361 | MS.gene25301:CDS |
| AGGGAGCCTAATCGCGTTGT+TGG | 0.448678 | 8.2:+38247110 | MS.gene25301:CDS |
| CAGTATGCACGTGATGTAGA+TGG | 0.451140 | 8.2:+38247248 | MS.gene25301:CDS |
| GGTTATATAAAGGATTATGA+TGG | 0.483037 | 8.2:+38247371 | MS.gene25301:CDS |
| CATCCAGCCTCTTTCAAACC+AGG | 0.501793 | 8.2:-38247590 | None:intergenic |
| ATCCCTGGTTTGAAAGAGGC+TGG | 0.505140 | 8.2:+38247587 | MS.gene25301:CDS |
| CAGAAGAGATCAGAAGCTGC+AGG | 0.509224 | 8.2:+38247539 | MS.gene25301:CDS |
| ACGCCTCCAACAACGCGATT+AGG | 0.512140 | 8.2:-38247116 | None:intergenic |
| GAGCCTAATCGCGTTGTTGG+AGG | 0.512685 | 8.2:+38247113 | MS.gene25301:CDS |
| AGAATTGTTGATGACATCCC+TGG | 0.521290 | 8.2:+38247572 | MS.gene25301:CDS |
| TATGATCCGTCGGCAAAGAC+AGG | 0.524541 | 8.2:+38247451 | MS.gene25301:CDS |
| CTGATATGTACGGTCATTCG+CGG | 0.528433 | 8.2:+38247618 | MS.gene25301:CDS |
| GTTATATAAAGGATTATGAT+GGG | 0.531624 | 8.2:+38247372 | MS.gene25301:CDS |
| CTGGTTTGAAAGAGGCTGGA+TGG | 0.535917 | 8.2:+38247591 | MS.gene25301:CDS |
| TATATAAAGGATTATGATGG+GGG | 0.574524 | 8.2:+38247374 | MS.gene25301:CDS |
| ATCGTCCATATGCAAATCAA+AGG | 0.576246 | 8.2:+38247144 | MS.gene25301:CDS |
| TCACTTGGAGAGAGATCGAT+GGG | 0.581124 | 8.2:+38247346 | MS.gene25301:CDS |
| TGACATCCCTGGTTTGAAAG+AGG | 0.585348 | 8.2:+38247583 | MS.gene25301:CDS |
| ATTGGCTATTTCATCAAACA+AGG | 0.588299 | 8.2:+38247308 | MS.gene25301:CDS |
| TTCACTTGGAGAGAGATCGA+TGG | 0.592040 | 8.2:+38247345 | MS.gene25301:CDS |
| ATAGTGCCTGTCTTTGCCGA+CGG | 0.594963 | 8.2:-38247457 | None:intergenic |
| ATTTACAAGAGAGATTCACT+TGG | 0.595789 | 8.2:+38247331 | MS.gene25301:CDS |
| GGATGGACTCCTGATATGTA+CGG | 0.597833 | 8.2:+38247608 | MS.gene25301:CDS |
| TTGGAGAGAGATCGATGGGA+AGG | 0.618097 | 8.2:+38247350 | MS.gene25301:CDS |
| ATCCAGCCTCTTTCAAACCA+GGG | 0.635327 | 8.2:-38247589 | None:intergenic |
| TTATATAAAGGATTATGATG+GGG | 0.635609 | 8.2:+38247373 | MS.gene25301:CDS |
| ATTTATCAACTATGATCCGT+CGG | 0.639900 | 8.2:+38247441 | MS.gene25301:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTATATAAAGGATTATGAT+GGG | + | chr8.2:38247372-38247391 | MS.gene25301:CDS | 20.0% |
| !! | TTATATAAAGGATTATGATG+GGG | + | chr8.2:38247373-38247392 | MS.gene25301:CDS | 20.0% |
| !!! | ATAGTTGATAAATCAGTATA+AGG | - | chr8.2:38247434-38247453 | None:intergenic | 20.0% |
| ! | ATGTCATCAACAATTCTATT+AGG | - | chr8.2:38247569-38247588 | None:intergenic | 25.0% |
| ! | GGTTATATAAAGGATTATGA+TGG | + | chr8.2:38247371-38247390 | MS.gene25301:CDS | 25.0% |
| ! | TATATAAAGGATTATGATGG+GGG | + | chr8.2:38247374-38247393 | MS.gene25301:CDS | 25.0% |
| ! | TATGCTGATAATAATGCTAT+TGG | + | chr8.2:38247290-38247309 | MS.gene25301:CDS | 25.0% |
| AAATCAGTATAAGGAAGCTT+TGG | - | chr8.2:38247425-38247444 | None:intergenic | 30.0% | |
| AGTGATGATCAACAAGTAAA+AGG | + | chr8.2:38247194-38247213 | MS.gene25301:CDS | 30.0% | |
| ATTTACAAGAGAGATTCACT+TGG | + | chr8.2:38247331-38247350 | MS.gene25301:CDS | 30.0% | |
| ATTTATCAACTATGATCCGT+CGG | + | chr8.2:38247441-38247460 | MS.gene25301:CDS | 30.0% | |
| GATCAACAAGTAAAAGGTTA+TGG | + | chr8.2:38247200-38247219 | MS.gene25301:CDS | 30.0% | |
| TGTCACAATATTGTTTATCC+TGG | + | chr8.2:38247506-38247525 | MS.gene25301:CDS | 30.0% | |
| ! | ATTGGCTATTTCATCAAACA+AGG | + | chr8.2:38247308-38247327 | MS.gene25301:CDS | 30.0% |
| ATCGTCCATATGCAAATCAA+AGG | + | chr8.2:38247144-38247163 | MS.gene25301:CDS | 35.0% | |
| TCGATGGGAAGGTTATATAA+AGG | + | chr8.2:38247361-38247380 | MS.gene25301:CDS | 35.0% | |
| CTCTTCTGAACATCAATTCC+AGG | - | chr8.2:38247527-38247546 | None:intergenic | 40.0% | |
| !! | AGAATTGTTGATGACATCCC+TGG | + | chr8.2:38247572-38247591 | MS.gene25301:CDS | 40.0% |
| !! | GCGAACCTTTGATTTGCATA+TGG | - | chr8.2:38247152-38247171 | None:intergenic | 40.0% |
| ATCCAGCCTCTTTCAAACCA+GGG | - | chr8.2:38247592-38247611 | None:intergenic | 45.0% | |
| CAGTATGCACGTGATGTAGA+TGG | + | chr8.2:38247248-38247267 | MS.gene25301:CDS | 45.0% | |
| TCACTTGGAGAGAGATCGAT+GGG | + | chr8.2:38247346-38247365 | MS.gene25301:CDS | 45.0% | |
| TTCACTTGGAGAGAGATCGA+TGG | + | chr8.2:38247345-38247364 | MS.gene25301:CDS | 45.0% | |
| ! | GGATGGACTCCTGATATGTA+CGG | + | chr8.2:38247608-38247627 | MS.gene25301:CDS | 45.0% |
| ! | TGACATCCCTGGTTTGAAAG+AGG | + | chr8.2:38247583-38247602 | MS.gene25301:CDS | 45.0% |
| ! | TTATGATGGGGGAACTCTCA+TGG | + | chr8.2:38247385-38247404 | MS.gene25301:CDS | 45.0% |
| ATAGTGCCTGTCTTTGCCGA+CGG | - | chr8.2:38247460-38247479 | None:intergenic | 50.0% | |
| CAGAAGAGATCAGAAGCTGC+AGG | + | chr8.2:38247539-38247558 | MS.gene25301:CDS | 50.0% | |
| CATCCAGCCTCTTTCAAACC+AGG | - | chr8.2:38247593-38247612 | None:intergenic | 50.0% | |
| CGCGAATGACCGTACATATC+AGG | - | chr8.2:38247620-38247639 | None:intergenic | 50.0% | |
| TATGATCCGTCGGCAAAGAC+AGG | + | chr8.2:38247451-38247470 | MS.gene25301:CDS | 50.0% | |
| TTGGAGAGAGATCGATGGGA+AGG | + | chr8.2:38247350-38247369 | MS.gene25301:CDS | 50.0% | |
| ! | ATCCCTGGTTTGAAAGAGGC+TGG | + | chr8.2:38247587-38247606 | MS.gene25301:CDS | 50.0% |
| ! | CTGGTTTGAAAGAGGCTGGA+TGG | + | chr8.2:38247591-38247610 | MS.gene25301:CDS | 50.0% |
| ACGCCTCCAACAACGCGATT+AGG | - | chr8.2:38247119-38247138 | None:intergenic | 55.0% | |
| AGGGAGCCTAATCGCGTTGT+TGG | + | chr8.2:38247110-38247129 | MS.gene25301:CDS | 55.0% | |
| GAGCCTAATCGCGTTGTTGG+AGG | + | chr8.2:38247113-38247132 | MS.gene25301:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 38247095 | 38247639 | 38247095 | ID=MS.gene25301 |
| chr8.2 | mRNA | 38247095 | 38247639 | 38247095 | ID=MS.gene25301.t1;Parent=MS.gene25301 |
| chr8.2 | exon | 38247095 | 38247639 | 38247095 | ID=MS.gene25301.t1.exon1;Parent=MS.gene25301.t1 |
| chr8.2 | CDS | 38247095 | 38247639 | 38247095 | ID=cds.MS.gene25301.t1;Parent=MS.gene25301.t1 |
| Gene Sequence |
| Protein sequence |
