Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25304.t1 | XP_003628592.1 | 92.6 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 1.30E-245 | 858.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25304.t1 | Q9AR19 | 66.3 | 403 | 130 | 4 | 46 | 446 | 170 | 568 | 6.6e-158 | 558.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25304.t1 | G7LEM3 | 92.6 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 9.3e-246 | 858.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25304.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25304.t1 | MTR_8g062330 | 92.617 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 0.0 | 860 |
| MS.gene25304.t1 | MTR_7g096040 | 75.556 | 405 | 93 | 5 | 43 | 446 | 143 | 542 | 0.0 | 644 |
| MS.gene25304.t1 | MTR_2g018270 | 41.935 | 93 | 51 | 2 | 344 | 434 | 85 | 176 | 6.70e-13 | 70.9 |
| MS.gene25304.t1 | MTR_8g005100 | 42.857 | 84 | 46 | 2 | 358 | 441 | 98 | 179 | 2.90e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25304.t1 | AT3G54610 | 66.917 | 399 | 121 | 5 | 50 | 446 | 179 | 568 | 0.0 | 574 |
| MS.gene25304.t1 | AT2G34900 | 34.906 | 106 | 65 | 3 | 331 | 434 | 104 | 207 | 7.34e-12 | 67.4 |
| MS.gene25304.t1 | AT2G34900 | 34.694 | 98 | 61 | 2 | 344 | 439 | 6 | 102 | 8.98e-12 | 65.9 |
| MS.gene25304.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 1.16e-11 | 67.0 |
| MS.gene25304.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 1.16e-11 | 67.0 |
| MS.gene25304.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 4.46e-11 | 65.9 |
| MS.gene25304.t1 | AT3G01770 | 27.317 | 205 | 115 | 7 | 251 | 438 | 43 | 230 | 5.07e-11 | 65.1 |
Find 0 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGTCTATAATGGTTATA+AGG | - | 57426:12610-12629 | MS.gene25304:CDS | 20.0% |
| !! | GTTATATAAAGGATTATGAT+GGG | - | 57426:12892-12911 | MS.gene25304:CDS | 20.0% |
| !! | TAAGTCTATAATGGTTATAA+GGG | - | 57426:12611-12630 | MS.gene25304:CDS | 20.0% |
| !! | TGAAATCATAAAAAATCCAA+TGG | - | 57426:13310-13329 | MS.gene25304:CDS | 20.0% |
| !! | TTATAACCATTATAGACTTA+TGG | + | 57426:12611-12630 | None:intergenic | 20.0% |
| !! | TTATATAAAGGATTATGATG+GGG | - | 57426:12893-12912 | MS.gene25304:CDS | 20.0% |
| !! | TTTATGATTTCATAATAACC+AGG | + | 57426:13302-13321 | None:intergenic | 20.0% |
| !!! | ATAGTTGATAAATCAGTATA+AGG | + | 57426:12954-12973 | None:intergenic | 20.0% |
| !!! | CATTTGTAATATATGGTTTT+TGG | + | 57426:13446-13465 | None:intergenic | 20.0% |
| !!! | TAAACTCAGTTAAATGTTTT+AGG | + | 57426:13202-13221 | None:intergenic | 20.0% |
| ! | ATGTCATCAACAATTCTATT+AGG | + | 57426:13089-13108 | None:intergenic | 25.0% |
| ! | GGTTATATAAAGGATTATGA+TGG | - | 57426:12891-12910 | MS.gene25304:CDS | 25.0% |
| ! | TATATAAAGGATTATGATGG+GGG | - | 57426:12894-12913 | MS.gene25304:CDS | 25.0% |
| ! | TATGCTGATAATAATGCTAT+TGG | - | 57426:12810-12829 | MS.gene25304:CDS | 25.0% |
| ! | TGTAGCACATTTGTAATATA+TGG | + | 57426:13453-13472 | None:intergenic | 25.0% |
| !! | TTCAGTGAATATATTTGTTG+CGG | + | 57426:12385-12404 | None:intergenic | 25.0% |
| !!! | AATGTTTTAGGTTTGTAACA+TGG | + | 57426:13190-13209 | None:intergenic | 25.0% |
| !!! | TTGTAATATATGGTTTTTGG+AGG | + | 57426:13443-13462 | None:intergenic | 25.0% |
| AAATCAGTATAAGGAAGCTT+TGG | + | 57426:12945-12964 | None:intergenic | 30.0% | |
| AGTGATGATCAACAAGTAAA+AGG | - | 57426:12714-12733 | MS.gene25304:CDS | 30.0% | |
| ATTTACAAGAGAGATTCACT+TGG | - | 57426:12851-12870 | MS.gene25304:CDS | 30.0% | |
| ATTTATCAACTATGATCCGT+CGG | - | 57426:12961-12980 | MS.gene25304:CDS | 30.0% | |
| GATCAACAAGTAAAAGGTTA+TGG | - | 57426:12720-12739 | MS.gene25304:CDS | 30.0% | |
| GTTTATGCGTTCAATTCTAA+AGG | - | 57426:13217-13236 | MS.gene25304:CDS | 30.0% | |
| TAGGAACCATAAGTCTATAA+TGG | - | 57426:12602-12621 | MS.gene25304:CDS | 30.0% | |
| TGTCACAATATTGTTTATCC+TGG | - | 57426:13026-13045 | MS.gene25304:CDS | 30.0% | |
| ! | ATTGGCTATTTCATCAAACA+AGG | - | 57426:12828-12847 | MS.gene25304:CDS | 30.0% |
| !! | TATGGTTTTTGGAGGATTAT+AGG | + | 57426:13435-13454 | None:intergenic | 30.0% |
| ACTGAAAAATTGCAGACAAG+AGG | - | 57426:12399-12418 | MS.gene25304:CDS | 35.0% | |
| ATCGTCCATATGCAAATCAA+AGG | - | 57426:12664-12683 | MS.gene25304:CDS | 35.0% | |
| ATCTAAAGACAATGAGTGAG+AGG | - | 57426:13333-13352 | MS.gene25304:CDS | 35.0% | |
| ATGCTATCAACGAAACCATA+TGG | + | 57426:12515-12534 | None:intergenic | 35.0% | |
| CTCATTGTCTTTAGATCCAT+TGG | + | 57426:13329-13348 | None:intergenic | 35.0% | |
| CTTAAGAAAGAGGAAGAATC+TGG | - | 57426:12447-12466 | MS.gene25304:CDS | 35.0% | |
| TCGATGGGAAGGTTATATAA+AGG | - | 57426:12881-12900 | MS.gene25304:CDS | 35.0% | |
| TGCATTCTCAAACATTCTTC+TGG | + | 57426:13408-13427 | None:intergenic | 35.0% | |
| !! | TGTCACTTTGCTTTGAAAGT+GGG | + | 57426:13486-13505 | None:intergenic | 35.0% |
| !! | TTGTCACTTTGCTTTGAAAG+TGG | + | 57426:13487-13506 | None:intergenic | 35.0% |
| CTCAAACATTCTTCTGGCAT+CGG | + | 57426:13402-13421 | None:intergenic | 40.0% | |
| CTCTTCTGAACATCAATTCC+AGG | + | 57426:13047-13066 | None:intergenic | 40.0% | |
| GAAAAATTGCAGACAAGAGG+TGG | - | 57426:12402-12421 | MS.gene25304:CDS | 40.0% | |
| GGAAGAGACTCTTAAGAAAG+AGG | - | 57426:12437-12456 | MS.gene25304:CDS | 40.0% | |
| GGTTCCTATCCATAACAAGT+CGG | + | 57426:12590-12609 | None:intergenic | 40.0% | |
| TTCCTCCTTAACCACAAAGT+CGG | + | 57426:12361-12380 | None:intergenic | 40.0% | |
| TTTCTTAAGAGTCTCTTCCC+TGG | + | 57426:12436-12455 | None:intergenic | 40.0% | |
| TTTGAATGCGTGTGCAATGA+TGG | - | 57426:12480-12499 | MS.gene25304:CDS | 40.0% | |
| !! | AGAATTGTTGATGACATCCC+TGG | - | 57426:13092-13111 | MS.gene25304:CDS | 40.0% |
| !! | GCGAACCTTTGATTTGCATA+TGG | + | 57426:12672-12691 | None:intergenic | 40.0% |
| !! | GTACATTGTCCGACTTGTTA+TGG | - | 57426:12578-12597 | MS.gene25304:CDS | 40.0% |
| !! | TGATGGTGTTGATGACCATA+TGG | - | 57426:12497-12516 | MS.gene25304:CDS | 40.0% |
| !! | TTGTCCGACTTGTTATGGAT+AGG | - | 57426:12583-12602 | MS.gene25304:CDS | 40.0% |
| !!! | ACTGAATTTTGAAGCCAGAC+TGG | + | 57426:13517-13536 | None:intergenic | 40.0% |
| AAAGACAATGAGTGAGAGGC+TGG | - | 57426:13337-13356 | MS.gene25304:CDS | 45.0% | |
| AAGAAGTGGTGAAGGCAGAA+GGG | + | 57426:12215-12234 | None:intergenic | 45.0% | |
| AAGTCGGACAATGTACTCCT+TGG | + | 57426:12574-12593 | None:intergenic | 45.0% | |
| AATGTACTCCTTGGGCATGT+TGG | + | 57426:12565-12584 | None:intergenic | 45.0% | |
| AGTCGGACAATGTACTCCTT+GGG | + | 57426:12573-12592 | None:intergenic | 45.0% | |
| ATCCAGCCTCTTTCAAACCA+GGG | + | 57426:13112-13131 | None:intergenic | 45.0% | |
| ATGTACTCCTTGGGCATGTT+GGG | + | 57426:12564-12583 | None:intergenic | 45.0% | |
| CAAGCGCAAACATGTATCTG+AGG | - | 57426:12242-12261 | MS.gene25304:CDS | 45.0% | |
| CAGATACATGTTTGCGCTTG+TGG | + | 57426:12242-12261 | None:intergenic | 45.0% | |
| CAGTATGCACGTGATGTAGA+TGG | - | 57426:12768-12787 | MS.gene25304:CDS | 45.0% | |
| CTGATATGTACGGTCATTCG+CGG | - | 57426:13138-13157 | MS.gene25304:CDS | 45.0% | |
| GCATCAACAGGTTCCTTGAA+TGG | + | 57426:13269-13288 | None:intergenic | 45.0% | |
| TACATGTTTGCGCTTGTGGA+TGG | + | 57426:12238-12257 | None:intergenic | 45.0% | |
| TCACTTGGAGAGAGATCGAT+GGG | - | 57426:12866-12885 | MS.gene25304:CDS | 45.0% | |
| TCCTCCTTAACCACAAAGTC+GGG | + | 57426:12360-12379 | None:intergenic | 45.0% | |
| TCTTCGATGCACAACTTCAC+CGG | - | 57426:12297-12316 | MS.gene25304:CDS | 45.0% | |
| TTCACTTGGAGAGAGATCGA+TGG | - | 57426:12865-12884 | MS.gene25304:CDS | 45.0% | |
| ! | GGATGGACTCCTGATATGTA+CGG | - | 57426:13128-13147 | MS.gene25304:CDS | 45.0% |
| ! | TCATTCGCGGTTTAGAGCTT+TGG | - | 57426:13151-13170 | MS.gene25304:CDS | 45.0% |
| ! | TGACATCCCTGGTTTGAAAG+AGG | - | 57426:13103-13122 | MS.gene25304:CDS | 45.0% |
| ! | TTATGATGGGGGAACTCTCA+TGG | - | 57426:12905-12924 | MS.gene25304:CDS | 45.0% |
| !!! | GAATTTTGAAGCCAGACTGG+AGG | + | 57426:13514-13533 | None:intergenic | 45.0% |
| AATGAGTGAGAGGCTGGAGT+CGG | - | 57426:13343-13362 | MS.gene25304:CDS | 50.0% | |
| ACAACTTCCCAACATGCCCA+AGG | - | 57426:12554-12573 | MS.gene25304:CDS | 50.0% | |
| ATAGTGCCTGTCTTTGCCGA+CGG | + | 57426:12980-12999 | None:intergenic | 50.0% | |
| CAGAAGAGATCAGAAGCTGC+AGG | - | 57426:13059-13078 | MS.gene25304:CDS | 50.0% | |
| CATCCAGCCTCTTTCAAACC+AGG | + | 57426:13113-13132 | None:intergenic | 50.0% | |
| CCTCCTTAACCACAAAGTCG+GGG | + | 57426:12359-12378 | None:intergenic | 50.0% | |
| CCTTGAATGGCCAAGCATCA+GGG | + | 57426:13256-13275 | None:intergenic | 50.0% | |
| CGCGAATGACCGTACATATC+AGG | + | 57426:13140-13159 | None:intergenic | 50.0% | |
| GAAGAAGTGGTGAAGGCAGA+AGG | + | 57426:12216-12235 | None:intergenic | 50.0% | |
| GCATCGAAGAGTCGCAAGAA+GGG | + | 57426:12287-12306 | None:intergenic | 50.0% | |
| GGAACACTCTGTGCATCAAC+AGG | + | 57426:13281-13300 | None:intergenic | 50.0% | |
| GGCTGAACTGTTGACAGAAC+CGG | + | 57426:12319-12338 | None:intergenic | 50.0% | |
| GTGGATGGAAGAAGTGGTGA+AGG | + | 57426:12223-12242 | None:intergenic | 50.0% | |
| TATGATCCGTCGGCAAAGAC+AGG | - | 57426:12971-12990 | MS.gene25304:CDS | 50.0% | |
| TCCTTGAATGGCCAAGCATC+AGG | + | 57426:13257-13276 | None:intergenic | 50.0% | |
| TGCATCGAAGAGTCGCAAGA+AGG | + | 57426:12288-12307 | None:intergenic | 50.0% | |
| TGCATGATCACCCTGATGCT+TGG | - | 57426:13243-13262 | MS.gene25304:CDS | 50.0% | |
| TGTCCCCGACTTTGTGGTTA+AGG | - | 57426:12353-12372 | MS.gene25304:CDS | 50.0% | |
| TTGGAGAGAGATCGATGGGA+AGG | - | 57426:12870-12889 | MS.gene25304:CDS | 50.0% | |
| ! | ATCCCTGGTTTGAAAGAGGC+TGG | - | 57426:13107-13126 | MS.gene25304:CDS | 50.0% |
| ! | CTGGTTTGAAAGAGGCTGGA+TGG | - | 57426:13111-13130 | MS.gene25304:CDS | 50.0% |
| ! | GTTGATGCACAGAGTGTTCC+TGG | - | 57426:13281-13300 | MS.gene25304:CDS | 50.0% |
| !! | TCGCGGTTTAGAGCTTTGGA+TGG | - | 57426:13155-13174 | MS.gene25304:CDS | 50.0% |
| AACAGTTCAGCCGCCACTTC+CGG | - | 57426:12327-12346 | MS.gene25304:CDS | 55.0% | |
| AAGAGGTGGCTACAGTGCCA+GGG | - | 57426:12416-12435 | MS.gene25304:CDS | 55.0% | |
| ACGCCTCCAACAACGCGATT+AGG | + | 57426:12639-12658 | None:intergenic | 55.0% | |
| AGGGAGCCTAATCGCGTTGT+TGG | - | 57426:12630-12649 | MS.gene25304:CDS | 55.0% | |
| CACAAAGTCGGGGACAGAAC+CGG | + | 57426:12349-12368 | None:intergenic | 55.0% | |
| CCCCGACTTTGTGGTTAAGG+AGG | - | 57426:12356-12375 | MS.gene25304:CDS | 55.0% | |
| CCCTGATGCTTGGCCATTCA+AGG | - | 57426:13253-13272 | MS.gene25304:CDS | 55.0% | |
| GAGCCTAATCGCGTTGTTGG+AGG | - | 57426:12633-12652 | MS.gene25304:CDS | 55.0% | |
| GAGTCGCAAGAAGGGTGGAA+TGG | + | 57426:12279-12298 | None:intergenic | 55.0% | |
| GCGCTTGTGGATGGAAGAAG+TGG | + | 57426:12229-12248 | None:intergenic | 55.0% | |
| GTGACAACACTCCTCCAGTC+TGG | - | 57426:13500-13519 | MS.gene25304:CDS | 55.0% | |
| TCGAAGAGTCGCAAGAAGGG+TGG | + | 57426:12284-12303 | None:intergenic | 55.0% | |
| TCGCAAGAAGGGTGGAATGG+AGG | + | 57426:12276-12295 | None:intergenic | 55.0% | |
| CAAGAGGTGGCTACAGTGCC+AGG | - | 57426:12415-12434 | MS.gene25304:CDS | 60.0% | |
| CGGTTCTGTCCCCGACTTTG+TGG | - | 57426:12347-12366 | MS.gene25304:CDS | 60.0% | |
| ! | TGGTGAAGGCAGAAGGGCAC+TGG | + | 57426:12209-12228 | None:intergenic | 60.0% |
| !! | GGTGAAGGCAGAAGGGCACT+GGG | + | 57426:12208-12227 | None:intergenic | 60.0% |
| GGGGACAGAACCGGAAGTGG+CGG | + | 57426:12340-12359 | None:intergenic | 65.0% | |
| GTCGGGGACAGAACCGGAAG+TGG | + | 57426:12343-12362 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57426 | gene | 12198 | 13538 | 12198 | ID=MS.gene25304 |
| 57426 | mRNA | 12198 | 13538 | 12198 | ID=MS.gene25304.t1;Parent=MS.gene25304 |
| 57426 | exon | 12198 | 13538 | 12198 | ID=MS.gene25304.t1.exon1;Parent=MS.gene25304.t1 |
| 57426 | CDS | 12198 | 13538 | 12198 | ID=cds.MS.gene25304.t1;Parent=MS.gene25304.t1 |
| Gene Sequence |
| Protein sequence |
