Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25305.t1 | XP_003628592.1 | 92.6 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 1.30E-245 | 858.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25305.t1 | Q9AR19 | 66.3 | 403 | 130 | 4 | 46 | 446 | 170 | 568 | 6.6e-158 | 558.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25305.t1 | G7LEM3 | 92.6 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 9.3e-246 | 858.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25305.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25305.t1 | MTR_8g062330 | 92.617 | 447 | 32 | 1 | 1 | 446 | 1 | 447 | 0.0 | 860 |
| MS.gene25305.t1 | MTR_7g096040 | 75.556 | 405 | 93 | 5 | 43 | 446 | 143 | 542 | 0.0 | 644 |
| MS.gene25305.t1 | MTR_2g018270 | 41.935 | 93 | 51 | 2 | 344 | 434 | 85 | 176 | 6.70e-13 | 70.9 |
| MS.gene25305.t1 | MTR_8g005100 | 42.857 | 84 | 46 | 2 | 358 | 441 | 98 | 179 | 2.90e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25305.t1 | AT3G54610 | 66.917 | 399 | 121 | 5 | 50 | 446 | 179 | 568 | 0.0 | 574 |
| MS.gene25305.t1 | AT2G34900 | 34.906 | 106 | 65 | 3 | 331 | 434 | 104 | 207 | 7.34e-12 | 67.4 |
| MS.gene25305.t1 | AT2G34900 | 34.694 | 98 | 61 | 2 | 344 | 439 | 6 | 102 | 8.98e-12 | 65.9 |
| MS.gene25305.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 1.16e-11 | 67.0 |
| MS.gene25305.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 1.16e-11 | 67.0 |
| MS.gene25305.t1 | AT5G63320 | 34.783 | 92 | 57 | 2 | 344 | 433 | 167 | 257 | 4.46e-11 | 65.9 |
| MS.gene25305.t1 | AT3G01770 | 27.317 | 205 | 115 | 7 | 251 | 438 | 43 | 230 | 5.07e-11 | 65.1 |
Find 0 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGTCTATAATGGTTATA+AGG | - | 57425:1932-1951 | MS.gene25305:CDS | 20.0% |
| !! | GTTATATAAAGGATTATGAT+GGG | - | 57425:2214-2233 | MS.gene25305:CDS | 20.0% |
| !! | TAAGTCTATAATGGTTATAA+GGG | - | 57425:1933-1952 | MS.gene25305:CDS | 20.0% |
| !! | TGAAATCATAAAAAATCCAA+TGG | - | 57425:2632-2651 | MS.gene25305:CDS | 20.0% |
| !! | TTATAACCATTATAGACTTA+TGG | + | 57425:1933-1952 | None:intergenic | 20.0% |
| !! | TTATATAAAGGATTATGATG+GGG | - | 57425:2215-2234 | MS.gene25305:CDS | 20.0% |
| !! | TTTATGATTTCATAATAACC+AGG | + | 57425:2624-2643 | None:intergenic | 20.0% |
| !!! | ATAGTTGATAAATCAGTATA+AGG | + | 57425:2276-2295 | None:intergenic | 20.0% |
| !!! | CATTTGTAATATATGGTTTT+TGG | + | 57425:2768-2787 | None:intergenic | 20.0% |
| !!! | TAAACTCAGTTAAATGTTTT+AGG | + | 57425:2524-2543 | None:intergenic | 20.0% |
| ! | ATGTCATCAACAATTCTATT+AGG | + | 57425:2411-2430 | None:intergenic | 25.0% |
| ! | GGTTATATAAAGGATTATGA+TGG | - | 57425:2213-2232 | MS.gene25305:CDS | 25.0% |
| ! | TATATAAAGGATTATGATGG+GGG | - | 57425:2216-2235 | MS.gene25305:CDS | 25.0% |
| ! | TATGCTGATAATAATGCTAT+TGG | - | 57425:2132-2151 | MS.gene25305:CDS | 25.0% |
| ! | TGTAGCACATTTGTAATATA+TGG | + | 57425:2775-2794 | None:intergenic | 25.0% |
| !! | TTCAGTGAATATATTTGTTG+CGG | + | 57425:1707-1726 | None:intergenic | 25.0% |
| !!! | AATGTTTTAGGTTTGTAACA+TGG | + | 57425:2512-2531 | None:intergenic | 25.0% |
| !!! | TTGTAATATATGGTTTTTGG+AGG | + | 57425:2765-2784 | None:intergenic | 25.0% |
| AAATCAGTATAAGGAAGCTT+TGG | + | 57425:2267-2286 | None:intergenic | 30.0% | |
| AGTGATGATCAACAAGTAAA+AGG | - | 57425:2036-2055 | MS.gene25305:CDS | 30.0% | |
| ATTTACAAGAGAGATTCACT+TGG | - | 57425:2173-2192 | MS.gene25305:CDS | 30.0% | |
| ATTTATCAACTATGATCCGT+CGG | - | 57425:2283-2302 | MS.gene25305:CDS | 30.0% | |
| GATCAACAAGTAAAAGGTTA+TGG | - | 57425:2042-2061 | MS.gene25305:CDS | 30.0% | |
| GTTTATGCGTTCAATTCTAA+AGG | - | 57425:2539-2558 | MS.gene25305:CDS | 30.0% | |
| TAGGAACCATAAGTCTATAA+TGG | - | 57425:1924-1943 | MS.gene25305:CDS | 30.0% | |
| TGTCACAATATTGTTTATCC+TGG | - | 57425:2348-2367 | MS.gene25305:CDS | 30.0% | |
| ! | ATTGGCTATTTCATCAAACA+AGG | - | 57425:2150-2169 | MS.gene25305:CDS | 30.0% |
| !! | TATGGTTTTTGGAGGATTAT+AGG | + | 57425:2757-2776 | None:intergenic | 30.0% |
| ACTGAAAAATTGCAGACAAG+AGG | - | 57425:1721-1740 | MS.gene25305:CDS | 35.0% | |
| ATCGTCCATATGCAAATCAA+AGG | - | 57425:1986-2005 | MS.gene25305:CDS | 35.0% | |
| ATCTAAAGACAATGAGTGAG+AGG | - | 57425:2655-2674 | MS.gene25305:CDS | 35.0% | |
| ATGCTATCAACGAAACCATA+TGG | + | 57425:1837-1856 | None:intergenic | 35.0% | |
| CTCATTGTCTTTAGATCCAT+TGG | + | 57425:2651-2670 | None:intergenic | 35.0% | |
| CTTAAGAAAGAGGAAGAATC+TGG | - | 57425:1769-1788 | MS.gene25305:CDS | 35.0% | |
| TCGATGGGAAGGTTATATAA+AGG | - | 57425:2203-2222 | MS.gene25305:CDS | 35.0% | |
| TGCATTCTCAAACATTCTTC+TGG | + | 57425:2730-2749 | None:intergenic | 35.0% | |
| !! | TGTCACTTTGCTTTGAAAGT+GGG | + | 57425:2808-2827 | None:intergenic | 35.0% |
| !! | TTGTCACTTTGCTTTGAAAG+TGG | + | 57425:2809-2828 | None:intergenic | 35.0% |
| CTCAAACATTCTTCTGGCAT+CGG | + | 57425:2724-2743 | None:intergenic | 40.0% | |
| CTCTTCTGAACATCAATTCC+AGG | + | 57425:2369-2388 | None:intergenic | 40.0% | |
| GAAAAATTGCAGACAAGAGG+TGG | - | 57425:1724-1743 | MS.gene25305:CDS | 40.0% | |
| GGAAGAGACTCTTAAGAAAG+AGG | - | 57425:1759-1778 | MS.gene25305:CDS | 40.0% | |
| GGTTCCTATCCATAACAAGT+CGG | + | 57425:1912-1931 | None:intergenic | 40.0% | |
| TTCCTCCTTAACCACAAAGT+CGG | + | 57425:1683-1702 | None:intergenic | 40.0% | |
| TTTCTTAAGAGTCTCTTCCC+TGG | + | 57425:1758-1777 | None:intergenic | 40.0% | |
| TTTGAATGCGTGTGCAATGA+TGG | - | 57425:1802-1821 | MS.gene25305:CDS | 40.0% | |
| !! | AGAATTGTTGATGACATCCC+TGG | - | 57425:2414-2433 | MS.gene25305:CDS | 40.0% |
| !! | GCGAACCTTTGATTTGCATA+TGG | + | 57425:1994-2013 | None:intergenic | 40.0% |
| !! | GTACATTGTCCGACTTGTTA+TGG | - | 57425:1900-1919 | MS.gene25305:CDS | 40.0% |
| !! | TGATGGTGTTGATGACCATA+TGG | - | 57425:1819-1838 | MS.gene25305:CDS | 40.0% |
| !! | TTGTCCGACTTGTTATGGAT+AGG | - | 57425:1905-1924 | MS.gene25305:CDS | 40.0% |
| !!! | ACTGAATTTTGAAGCCAGAC+TGG | + | 57425:2839-2858 | None:intergenic | 40.0% |
| AAAGACAATGAGTGAGAGGC+TGG | - | 57425:2659-2678 | MS.gene25305:CDS | 45.0% | |
| AAGAAGTGGTGAAGGCAGAA+GGG | + | 57425:1537-1556 | None:intergenic | 45.0% | |
| AAGTCGGACAATGTACTCCT+TGG | + | 57425:1896-1915 | None:intergenic | 45.0% | |
| AATGTACTCCTTGGGCATGT+TGG | + | 57425:1887-1906 | None:intergenic | 45.0% | |
| AGTCGGACAATGTACTCCTT+GGG | + | 57425:1895-1914 | None:intergenic | 45.0% | |
| ATCCAGCCTCTTTCAAACCA+GGG | + | 57425:2434-2453 | None:intergenic | 45.0% | |
| ATGTACTCCTTGGGCATGTT+GGG | + | 57425:1886-1905 | None:intergenic | 45.0% | |
| CAAGCGCAAACATGTATCTG+AGG | - | 57425:1564-1583 | MS.gene25305:CDS | 45.0% | |
| CAGATACATGTTTGCGCTTG+TGG | + | 57425:1564-1583 | None:intergenic | 45.0% | |
| CAGTATGCACGTGATGTAGA+TGG | - | 57425:2090-2109 | MS.gene25305:CDS | 45.0% | |
| CTGATATGTACGGTCATTCG+CGG | - | 57425:2460-2479 | MS.gene25305:CDS | 45.0% | |
| GCATCAACAGGTTCCTTGAA+TGG | + | 57425:2591-2610 | None:intergenic | 45.0% | |
| TACATGTTTGCGCTTGTGGA+TGG | + | 57425:1560-1579 | None:intergenic | 45.0% | |
| TCACTTGGAGAGAGATCGAT+GGG | - | 57425:2188-2207 | MS.gene25305:CDS | 45.0% | |
| TCCTCCTTAACCACAAAGTC+GGG | + | 57425:1682-1701 | None:intergenic | 45.0% | |
| TCTTCGATGCACAACTTCAC+CGG | - | 57425:1619-1638 | MS.gene25305:CDS | 45.0% | |
| TTCACTTGGAGAGAGATCGA+TGG | - | 57425:2187-2206 | MS.gene25305:CDS | 45.0% | |
| ! | GGATGGACTCCTGATATGTA+CGG | - | 57425:2450-2469 | MS.gene25305:CDS | 45.0% |
| ! | TCATTCGCGGTTTAGAGCTT+TGG | - | 57425:2473-2492 | MS.gene25305:CDS | 45.0% |
| ! | TGACATCCCTGGTTTGAAAG+AGG | - | 57425:2425-2444 | MS.gene25305:CDS | 45.0% |
| ! | TTATGATGGGGGAACTCTCA+TGG | - | 57425:2227-2246 | MS.gene25305:CDS | 45.0% |
| !!! | GAATTTTGAAGCCAGACTGG+AGG | + | 57425:2836-2855 | None:intergenic | 45.0% |
| AATGAGTGAGAGGCTGGAGT+CGG | - | 57425:2665-2684 | MS.gene25305:CDS | 50.0% | |
| ACAACTTCCCAACATGCCCA+AGG | - | 57425:1876-1895 | MS.gene25305:CDS | 50.0% | |
| ATAGTGCCTGTCTTTGCCGA+CGG | + | 57425:2302-2321 | None:intergenic | 50.0% | |
| CAGAAGAGATCAGAAGCTGC+AGG | - | 57425:2381-2400 | MS.gene25305:CDS | 50.0% | |
| CATCCAGCCTCTTTCAAACC+AGG | + | 57425:2435-2454 | None:intergenic | 50.0% | |
| CCTCCTTAACCACAAAGTCG+GGG | + | 57425:1681-1700 | None:intergenic | 50.0% | |
| CCTTGAATGGCCAAGCATCA+GGG | + | 57425:2578-2597 | None:intergenic | 50.0% | |
| CGCGAATGACCGTACATATC+AGG | + | 57425:2462-2481 | None:intergenic | 50.0% | |
| GAAGAAGTGGTGAAGGCAGA+AGG | + | 57425:1538-1557 | None:intergenic | 50.0% | |
| GCATCGAAGAGTCGCAAGAA+GGG | + | 57425:1609-1628 | None:intergenic | 50.0% | |
| GGAACACTCTGTGCATCAAC+AGG | + | 57425:2603-2622 | None:intergenic | 50.0% | |
| GGCTGAACTGTTGACAGAAC+CGG | + | 57425:1641-1660 | None:intergenic | 50.0% | |
| GTGGATGGAAGAAGTGGTGA+AGG | + | 57425:1545-1564 | None:intergenic | 50.0% | |
| TATGATCCGTCGGCAAAGAC+AGG | - | 57425:2293-2312 | MS.gene25305:CDS | 50.0% | |
| TCCTTGAATGGCCAAGCATC+AGG | + | 57425:2579-2598 | None:intergenic | 50.0% | |
| TGCATCGAAGAGTCGCAAGA+AGG | + | 57425:1610-1629 | None:intergenic | 50.0% | |
| TGCATGATCACCCTGATGCT+TGG | - | 57425:2565-2584 | MS.gene25305:CDS | 50.0% | |
| TGTCCCCGACTTTGTGGTTA+AGG | - | 57425:1675-1694 | MS.gene25305:CDS | 50.0% | |
| TTGGAGAGAGATCGATGGGA+AGG | - | 57425:2192-2211 | MS.gene25305:CDS | 50.0% | |
| ! | ATCCCTGGTTTGAAAGAGGC+TGG | - | 57425:2429-2448 | MS.gene25305:CDS | 50.0% |
| ! | CTGGTTTGAAAGAGGCTGGA+TGG | - | 57425:2433-2452 | MS.gene25305:CDS | 50.0% |
| ! | GTTGATGCACAGAGTGTTCC+TGG | - | 57425:2603-2622 | MS.gene25305:CDS | 50.0% |
| !! | TCGCGGTTTAGAGCTTTGGA+TGG | - | 57425:2477-2496 | MS.gene25305:CDS | 50.0% |
| AACAGTTCAGCCGCCACTTC+CGG | - | 57425:1649-1668 | MS.gene25305:CDS | 55.0% | |
| AAGAGGTGGCTACAGTGCCA+GGG | - | 57425:1738-1757 | MS.gene25305:CDS | 55.0% | |
| ACGCCTCCAACAACGCGATT+AGG | + | 57425:1961-1980 | None:intergenic | 55.0% | |
| AGGGAGCCTAATCGCGTTGT+TGG | - | 57425:1952-1971 | MS.gene25305:CDS | 55.0% | |
| CACAAAGTCGGGGACAGAAC+CGG | + | 57425:1671-1690 | None:intergenic | 55.0% | |
| CCCCGACTTTGTGGTTAAGG+AGG | - | 57425:1678-1697 | MS.gene25305:CDS | 55.0% | |
| CCCTGATGCTTGGCCATTCA+AGG | - | 57425:2575-2594 | MS.gene25305:CDS | 55.0% | |
| GAGCCTAATCGCGTTGTTGG+AGG | - | 57425:1955-1974 | MS.gene25305:CDS | 55.0% | |
| GAGTCGCAAGAAGGGTGGAA+TGG | + | 57425:1601-1620 | None:intergenic | 55.0% | |
| GCGCTTGTGGATGGAAGAAG+TGG | + | 57425:1551-1570 | None:intergenic | 55.0% | |
| GTGACAACACTCCTCCAGTC+TGG | - | 57425:2822-2841 | MS.gene25305:CDS | 55.0% | |
| TCGAAGAGTCGCAAGAAGGG+TGG | + | 57425:1606-1625 | None:intergenic | 55.0% | |
| TCGCAAGAAGGGTGGAATGG+AGG | + | 57425:1598-1617 | None:intergenic | 55.0% | |
| CAAGAGGTGGCTACAGTGCC+AGG | - | 57425:1737-1756 | MS.gene25305:CDS | 60.0% | |
| CGGTTCTGTCCCCGACTTTG+TGG | - | 57425:1669-1688 | MS.gene25305:CDS | 60.0% | |
| ! | TGGTGAAGGCAGAAGGGCAC+TGG | + | 57425:1531-1550 | None:intergenic | 60.0% |
| !! | GGTGAAGGCAGAAGGGCACT+GGG | + | 57425:1530-1549 | None:intergenic | 60.0% |
| GGGGACAGAACCGGAAGTGG+CGG | + | 57425:1662-1681 | None:intergenic | 65.0% | |
| GTCGGGGACAGAACCGGAAG+TGG | + | 57425:1665-1684 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57425 | gene | 1520 | 2860 | 1520 | ID=MS.gene25305 |
| 57425 | mRNA | 1520 | 2860 | 1520 | ID=MS.gene25305.t1;Parent=MS.gene25305 |
| 57425 | exon | 1520 | 2860 | 1520 | ID=MS.gene25305.t1.exon1;Parent=MS.gene25305.t1 |
| 57425 | CDS | 1520 | 2860 | 1520 | ID=cds.MS.gene25305.t1;Parent=MS.gene25305.t1 |
| Gene Sequence |
| Protein sequence |
