Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25311.t1 | AET03071.1 | 98.2 | 55 | 1 | 0 | 1 | 55 | 149 | 203 | 9.90E-23 | 115.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25311.t1 | O04539 | 45.3 | 53 | 29 | 0 | 1 | 53 | 111 | 163 | 7.2e-06 | 50.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25311.t1 | G7LEM6 | 98.2 | 55 | 1 | 0 | 1 | 55 | 149 | 203 | 7.1e-23 | 115.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25311.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25311.t1 | MTR_8g062350 | 98.182 | 55 | 1 | 0 | 1 | 55 | 149 | 203 | 3.75e-34 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATATTCTTTTAATCATG+TGG | - | 57422:5599-5618 | MS.gene25311:intron | 15.0% |
| !!! | ATATATTGTTGAACTTGTTT+AGG | - | 57422:5642-5661 | MS.gene25311:CDS | 20.0% |
| ! | CTAAATATTCTTACAAGAGA+GGG | + | 57422:5574-5593 | MS.gene25311:intergenic | 25.0% |
| ! | TCTAAATATTCTTACAAGAG+AGG | + | 57422:5575-5594 | MS.gene25311:intergenic | 25.0% |
| !!! | GAGTTAATTATGTTTTTTCC+TGG | - | 57422:5703-5722 | MS.gene25311:CDS | 25.0% |
| TCATGAACATGCTAATAGAT+AGG | - | 57422:5469-5488 | MS.gene25311:CDS | 30.0% | |
| TTATATCGAGACTTACTATG+AGG | - | 57422:5500-5519 | MS.gene25311:intron | 30.0% | |
| GAGAGGGAAGAGTCATAGAA+AGG | + | 57422:5558-5577 | MS.gene25310:intergenic | 45.0% | |
| ! | TGTTTAGGCTGCTGCACTTT+TGG | - | 57422:5657-5676 | MS.gene25311:CDS | 45.0% |
| ! | TAGGCTGCTGCACTTTTGGA+GGG | - | 57422:5661-5680 | MS.gene25311:CDS | 50.0% |
| ! | TTAGGCTGCTGCACTTTTGG+AGG | - | 57422:5660-5679 | MS.gene25311:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57422 | gene | 5423 | 5732 | 5423 | ID=MS.gene25311 |
| 57422 | mRNA | 5423 | 5732 | 5423 | ID=MS.gene25311.t1;Parent=MS.gene25311 |
| 57422 | exon | 5634 | 5732 | 5634 | ID=MS.gene25311.t1.exon1;Parent=MS.gene25311.t1 |
| 57422 | CDS | 5634 | 5732 | 5634 | ID=cds.MS.gene25311.t1;Parent=MS.gene25311.t1 |
| 57422 | exon | 5423 | 5491 | 5423 | ID=MS.gene25311.t1.exon2;Parent=MS.gene25311.t1 |
| 57422 | CDS | 5423 | 5491 | 5423 | ID=cds.MS.gene25311.t1;Parent=MS.gene25311.t1 |
| Gene Sequence |
| Protein sequence |
