AlfalfaGEDB | MS.gene25312

M. sativa cultivar XinJiangDaYe / MS.gene25312

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25312.t1	RHN41244.1	93.5	77	5	0	1	77	31	107	6.60E-33	149.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25312.t1	Q9LFQ3	51.3	78	35	1	2	76	37	114	2.4e-15	82.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25312.t1	G7LEM6	93.5	77	5	0	1	77	1	77	4.8e-33	149.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene25312.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25312.t1	MTR_8g062350	93.506	77	5	0	1	77	1	77	2.72e-48	151
MS.gene25312.t1	MTR_5g036670	55.556	72	32	0	5	76	34	105	8.33e-23	90.5
MS.gene25312.t1	MTR_8g062700	56.338	71	31	0	6	76	34	104	5.00e-22	88.2
MS.gene25312.t1	MTR_8g062700	56.338	71	31	0	6	76	34	104	5.04e-22	88.2
MS.gene25312.t1	MTR_6g008995	53.623	69	32	0	8	76	31	99	1.90e-19	80.5
MS.gene25312.t1	MTR_6g008970	53.333	75	32	1	2	76	54	125	2.67e-19	80.5
MS.gene25312.t1	MTR_1g105955	51.429	70	34	0	7	76	9	78	6.86e-19	76.3
MS.gene25312.t1	MTR_1g105955	48.438	64	33	0	13	76	94	157	7.61e-12	57.8
MS.gene25312.t1	MTR_3g006150	50.704	71	34	1	7	76	83	153	1.39e-16	69.7
MS.gene25312.t1	MTR_1g105960	51.613	62	30	0	15	76	2	63	2.83e-16	67.4
MS.gene25312.t1	MTR_3g006125	50.685	73	35	1	5	76	171	243	6.46e-16	69.7
MS.gene25312.t1	MTR_3g006125	52.113	71	33	1	8	77	83	153	3.12e-15	67.8
MS.gene25312.t1	MTR_3g006185	52.113	71	33	1	8	77	2	72	9.21e-16	67.8
MS.gene25312.t1	MTR_3g006185	49.296	71	35	1	7	76	91	161	1.99e-14	64.3
MS.gene25312.t1	MTR_3g006065	49.296	71	35	1	7	76	83	153	1.60e-15	67.4
MS.gene25312.t1	MTR_3g006105	52.113	71	33	1	8	77	2	72	2.24e-15	67.0
MS.gene25312.t1	MTR_3g006105	45.833	72	38	1	4	74	89	160	3.86e-12	58.5
MS.gene25312.t1	MTR_1g105010	43.056	72	41	0	6	77	8	79	6.37e-14	61.6
MS.gene25312.t1	MTR_1g104940	42.857	77	44	0	1	77	1	77	1.32e-13	61.2
MS.gene25312.t1	MTR_1g104960	41.096	73	43	0	4	76	89	161	1.91e-13	62.0
MS.gene25312.t1	MTR_4g133300	44.928	69	38	0	8	76	83	151	5.83e-13	61.6
MS.gene25312.t1	MTR_4g133300	47.143	70	35	1	7	76	167	234	4.08e-12	59.3
MS.gene25312.t1	MTR_4g133470	43.478	69	39	0	8	76	83	151	1.41e-12	60.5
MS.gene25312.t1	MTR_4g133470	47.143	70	35	1	7	76	167	234	1.04e-11	58.2
MS.gene25312.t1	MTR_7g013410	42.373	59	34	0	18	76	2	60	2.46e-12	58.2
MS.gene25312.t1	MTR_1g091047	43.939	66	36	1	13	77	8	73	4.98e-12	55.8
MS.gene25312.t1	MTR_2g097830	46.875	64	31	1	13	76	69	129	8.74e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25312.t1	AT1G24190	54.930	71	32	0	6	76	6	76	5.36e-21	85.1
MS.gene25312.t1	AT1G24190	54.930	71	32	0	6	76	6	76	5.73e-21	85.1
MS.gene25312.t1	AT1G24190	54.930	71	32	0	6	76	6	76	5.73e-21	85.1
MS.gene25312.t1	AT3G01320	53.623	69	32	0	8	76	51	119	2.92e-20	83.2
MS.gene25312.t1	AT3G01320	53.623	69	32	0	8	76	51	119	2.92e-20	83.2
MS.gene25312.t1	AT5G15020	53.623	69	32	0	8	76	46	114	4.14e-20	82.8
MS.gene25312.t1	AT5G15020	53.623	69	32	0	8	76	46	114	4.22e-20	82.8
MS.gene25312.t1	AT1G70060	50.000	76	38	0	1	76	1	76	1.91e-19	80.9
MS.gene25312.t1	AT1G70060	50.000	76	38	0	1	76	1	76	1.91e-19	80.9
MS.gene25312.t1	AT5G15025	53.731	67	31	0	10	76	43	109	1.37e-17	71.2
MS.gene25312.t1	AT1G59890	52.381	63	30	0	14	76	45	107	1.75e-17	75.1
MS.gene25312.t1	AT1G59890	52.381	63	30	0	14	76	45	107	1.77e-17	75.1
MS.gene25312.t1	AT1G59890	52.381	63	30	0	14	76	45	107	1.86e-17	75.1
MS.gene25312.t1	AT1G27220	44.737	76	42	0	1	76	1	76	1.90e-17	72.4
MS.gene25312.t1	AT1G59890	52.381	63	30	0	14	76	45	107	1.97e-17	75.1
MS.gene25312.t1	AT1G70030	45.333	75	41	0	3	77	11	85	2.29e-16	68.2
MS.gene25312.t1	AT1G27250	42.667	75	42	1	3	76	9	83	3.12e-16	68.2
MS.gene25312.t1	AT1G70030	45.333	75	41	0	3	77	11	85	4.10e-16	67.8
MS.gene25312.t1	AT1G70030	45.833	72	39	0	6	77	3	74	1.18e-15	67.4
MS.gene25312.t1	AT1G24200	45.455	77	42	0	1	77	1	77	1.60e-15	67.8
MS.gene25312.t1	AT1G10450	49.206	63	32	0	14	76	84	146	2.99e-15	68.9
MS.gene25312.t1	AT1G10450	49.206	63	32	0	14	76	33	95	3.10e-15	68.9
MS.gene25312.t1	AT1G10450	49.206	63	32	0	14	76	33	95	3.10e-15	68.9
MS.gene25312.t1	AT5G15040	49.180	61	31	0	17	77	6	66	8.16e-15	63.2
MS.gene25312.t1	AT5G15040	49.180	61	31	0	17	77	6	66	8.16e-15	63.2
MS.gene25312.t1	AT5G15040	49.180	61	31	0	17	77	6	66	8.16e-15	63.2
MS.gene25312.t1	AT1G24210	36.364	77	49	0	1	77	1	77	3.70e-14	63.2
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	2.27e-13	63.5
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	2.27e-13	63.5
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	237	300	2.48e-13	63.5
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	2.50e-13	63.5
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	2.50e-13	63.5
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	2.81e-13	63.2
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	2.81e-13	63.2
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	2.81e-13	63.2
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	3.19e-13	63.2
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	3.19e-13	63.2
MS.gene25312.t1	AT4G12020	46.154	65	34	1	10	74	304	367	3.20e-13	63.2
MS.gene25312.t1	AT1G23810	42.188	64	37	0	11	74	106	169	2.70e-12	59.7
MS.gene25312.t1	AT1G24250	42.188	64	37	0	11	74	117	180	2.57e-11	57.4

Find 0 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAGAAAAATGTAAATTAC+CGG	+	57422:12401-12420	MS.gene25312:intergenic	15.0%
!!	TGGTAAACTATAAATAATAT+AGG	+	57422:12434-12453	MS.gene25312:intergenic	15.0%
!!!	TTACTTTTTGAAAATAATGA+AGG	-	57422:12203-12222	MS.gene25312:intron	15.0%
!!	TAATTAGAGACGAAAAAAAT+TGG	+	57422:12375-12394	MS.gene25312:intergenic	20.0%
!	TAAATCACTGACCAATATTT+CGG	-	57422:12314-12333	MS.gene25312:intron	25.0%
!	TAATTACCATGATGTTGTAA+CGG	-	57422:12524-12543	MS.gene25312:CDS	25.0%
!	TCGCAAATAAAATTAAGATC+CGG	+	57422:12464-12483	MS.gene25312:intergenic	25.0%
!!!	ATTTTTTTCGTCCGAAATAT+TGG	+	57422:12328-12347	MS.gene25312:intergenic	25.0%
	AGGACATAATGATCTGATTA+CGG	-	57422:12566-12585	MS.gene25312:CDS	30.0%
	GATTACAAAGATAACAGGTT+AGG	-	57422:12225-12244	MS.gene25312:intron	30.0%
	TATTTATAGTTTACCACGAG+AGG	-	57422:12438-12457	MS.gene25312:intron	30.0%
	TGAAGGATTACAAAGATAAC+AGG	-	57422:12220-12239	MS.gene25312:intron	30.0%
!	TTTTCGTCTCTAATTAATGC+CGG	-	57422:12379-12398	MS.gene25312:intron	30.0%
	GGACATAATGATCTGATTAC+GGG	-	57422:12567-12586	MS.gene25312:CDS	35.0%
	GGATGTCTTCAAAGAACAAA+TGG	-	57422:12170-12189	MS.gene25312:CDS	35.0%
	TGATGCCTTTGCATATCTTA+AGG	-	57422:12140-12159	MS.gene25312:CDS	35.0%
!!	ATATGCAAAGGCATCATCTT+CGG	+	57422:12136-12155	MS.gene25312:intergenic	35.0%
	GACTGCCTTAAGATATGCAA+AGG	+	57422:12148-12167	MS.gene25312:intergenic	40.0%
	GAGGTGAAGGAGTTGTTTAA+AGG	-	57422:12546-12565	MS.gene25312:CDS	40.0%
	GCTAAGACAATCAAGAGTGA+TGG	+	57422:12279-12298	MS.gene25312:intergenic	40.0%
	TGCATATCTTAAGGCAGTCA+AGG	-	57422:12149-12168	MS.gene25312:CDS	40.0%
	TTACCATGATGTTGTAACGG+AGG	-	57422:12527-12546	MS.gene25312:CDS	40.0%
!	CAAGAGTGATGGAAAGAATG+TGG	+	57422:12268-12287	MS.gene25312:intergenic	40.0%
	TATAGTTTACCACGAGAGGC+CGG	-	57422:12442-12461	MS.gene25312:intron	45.0%
	TCACCTCCGTTACAACATCA+TGG	+	57422:12533-12552	MS.gene25312:intergenic	45.0%
!	TGATGTTGTAACGGAGGTGA+AGG	-	57422:12533-12552	MS.gene25312:CDS	45.0%
	AATTAAGATCCGGCCTCTCG+TGG	+	57422:12454-12473	MS.gene25311:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
57422	gene	12102	12612	12102	ID=MS.gene25312
57422	mRNA	12102	12612	12102	ID=MS.gene25312.t1;Parent=MS.gene25312
57422	exon	12473	12612	12473	ID=MS.gene25312.t1.exon1;Parent=MS.gene25312.t1
57422	CDS	12473	12612	12473	ID=cds.MS.gene25312.t1;Parent=MS.gene25312.t1
57422	exon	12102	12193	12102	ID=MS.gene25312.t1.exon2;Parent=MS.gene25312.t1
57422	CDS	12102	12193	12102	ID=cds.MS.gene25312.t1;Parent=MS.gene25312.t1

Gene Sequence

>MS.gene25312

ATGAGTACGAGTCAAGGTGGTAAGAAACTAACCGAAGATGATGCCTTTGCATATCTTAAGGCAGTCAAGGATGTCTTCAAAGAACAAATGGAAAACTATTCTTACTTTTTGAAAATAATGAAGGATTACAAAGATAACAGGTTAGGTCATTTGTTCATTTGTTCCACATTCTTTCCATCACTCTTGATTGTCTTAGCATAACTTCAAAATTTTAAATCACTGACCAATATTTCGGACGAAAAAAATAAGTAAAATAAATGTAAATTTAAACCAATTTTTTTCGTCTCTAATTAATGCCGGTAATTTACATTTTTCTATTTGTGCAATTTCCTATATTATTTATAGTTTACCACGAGAGGCCGGATCTTAATTTTATTTGCGAAAATTGACTTCAATATATTTATTTTGTTGATTTCTAGAATTAATTACCATGATGTTGTAACGGAGGTGAAGGAGTTGTTTAAAGGACATAATGATCTGATTACGGGATTCAACATCTTTGCACCAAGTG

Protein sequence

>MS.gene25312.t1

MSTSQGGKKLTEDDAFAYLKAVKDVFKEQMENYSYFLKIMKDYKDNRINYHDVVTEVKELFKGHNDLITGFNIFAPS