Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25758.t1 | KEH35169.1 | 90.2 | 122 | 11 | 1 | 1 | 121 | 52 | 173 | 3.80E-51 | 210.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25758.t1 | A0A072UZD5 | 90.2 | 122 | 11 | 1 | 1 | 121 | 52 | 173 | 2.7e-51 | 210.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25758.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25758.t1 | MTR_3g081210 | 90.164 | 122 | 11 | 1 | 1 | 121 | 52 | 173 | 2.86e-75 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAAACGAAATTGTTGTT+TGG | 0.350812 | 3.2:-71117623 | MS.gene25758:CDS |
| TGCAGGCTAGAGCTATAATA+TGG | 0.402146 | 3.2:-71117078 | MS.gene25758:intron |
| ATTTGACGAAAAGGAGCTTC+AGG | 0.413840 | 3.2:-71117424 | MS.gene25758:intron |
| ATGGTTGGCAAGTCAAGAAA+TGG | 0.426643 | 3.2:-71117059 | MS.gene25758:CDS |
| TCCTATTCTTTCTCTTCTCA+AGG | 0.460409 | 3.2:+71116918 | None:intergenic |
| CAAGAAATGGATGGAAAGAA+TGG | 0.480394 | 3.2:-71117046 | MS.gene25758:CDS |
| ATGCACTAGGTTGCATTGAA+AGG | 0.491779 | 3.2:+71116993 | None:intergenic |
| TGCACTAGGTTGCATTGAAA+GGG | 0.509370 | 3.2:+71116994 | None:intergenic |
| TGCATTGAAAGGGATAGAGT+TGG | 0.516749 | 3.2:+71117004 | None:intergenic |
| GGCTAGAGCTATAATATGGT+TGG | 0.541963 | 3.2:-71117074 | MS.gene25758:CDS |
| TCCTTGAGAAGAGAAAGAAT+AGG | 0.545631 | 3.2:-71116919 | MS.gene25758:CDS |
| ACACCACAGATTTGACGAAA+AGG | 0.545745 | 3.2:-71117433 | MS.gene25758:CDS |
| TTACAAATGCATGCCTCACA+TGG | 0.582863 | 3.2:-71116971 | MS.gene25758:CDS |
| CTTGTTGCAAATCCTCGCAA+TGG | 0.611244 | 3.2:-71117456 | MS.gene25758:CDS |
| TATCATGAGTTACAATAGAG+AGG | 0.622766 | 3.2:+71116882 | None:intergenic |
| AATCTGTGGTGTCCATTGCG+AGG | 0.627615 | 3.2:+71117444 | None:intergenic |
| TTGGCAAGTCAAGAAATGGA+TGG | 0.632410 | 3.2:-71117055 | MS.gene25758:CDS |
| GCATGCATTTGTAATGCACT+AGG | 0.632578 | 3.2:+71116980 | None:intergenic |
| CTTGATGGAAAAGCCATGTG+AGG | 0.674355 | 3.2:+71116958 | None:intergenic |
| CATGAGTTACAATAGAGAGG+TGG | 0.687315 | 3.2:+71116885 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATATCTATTTTTATCAT+TGG | - | chr3.2:71117365-71117384 | MS.gene25758:intron | 10.0% |
| !! | ATTTACATTATGAAAAAGAT+AGG | + | chr3.2:71117342-71117361 | None:intergenic | 15.0% |
| !!! | TTTTATCATTGGTTAAATTT+TGG | - | chr3.2:71117376-71117395 | MS.gene25758:intron | 15.0% |
| ! | AAAAAGATAGGTATATTCAC+TGG | + | chr3.2:71117330-71117349 | None:intergenic | 25.0% |
| !! | TATGGTTTTTGTTAAACTCT+TGG | - | chr3.2:71116956-71116975 | MS.gene25758:CDS | 25.0% |
| !!! | CTCTTGGAATTTTTTTTTGT+AGG | - | chr3.2:71116972-71116991 | MS.gene25758:CDS | 25.0% |
| ATACATTGATCATTACAGTG+TGG | + | chr3.2:71117261-71117280 | None:intergenic | 30.0% | |
| ATTATCGCATGCAAAAAGTT+AGG | + | chr3.2:71117167-71117186 | None:intergenic | 30.0% | |
| GATAGAATTTGAGTCGTTTA+TGG | - | chr3.2:71116938-71116957 | MS.gene25758:CDS | 30.0% | |
| TATCATGAGTTACAATAGAG+AGG | + | chr3.2:71117599-71117618 | None:intergenic | 30.0% | |
| TTGTTCTGTGGAAGAAAATT+TGG | - | chr3.2:71117123-71117142 | MS.gene25758:intron | 30.0% | |
| ! | AAATTTTCTTCCACAGAACA+AGG | + | chr3.2:71117124-71117143 | None:intergenic | 30.0% |
| !!! | ATTGGTTAAATTTTGGTTGC+AGG | - | chr3.2:71117383-71117402 | MS.gene25758:intron | 30.0% |
| ATTTCTTCATCCTTGTTCTG+TGG | - | chr3.2:71117111-71117130 | MS.gene25758:intron | 35.0% | |
| CAAGAAATGGATGGAAAGAA+TGG | - | chr3.2:71117432-71117451 | MS.gene25758:CDS | 35.0% | |
| CGTGTATGGCAAAAAAAACA+TGG | - | chr3.2:71117189-71117208 | MS.gene25758:intron | 35.0% | |
| TCCTATTCTTTCTCTTCTCA+AGG | + | chr3.2:71117563-71117582 | None:intergenic | 35.0% | |
| TCCTTGAGAAGAGAAAGAAT+AGG | - | chr3.2:71117559-71117578 | MS.gene25758:intron | 35.0% | |
| !!! | ATTTTGAAGTGATCGCTTGA+TGG | + | chr3.2:71117538-71117557 | None:intergenic | 35.0% |
| ACACCACAGATTTGACGAAA+AGG | - | chr3.2:71117045-71117064 | MS.gene25758:CDS | 40.0% | |
| ATGCACTAGGTTGCATTGAA+AGG | + | chr3.2:71117488-71117507 | None:intergenic | 40.0% | |
| ATGGTTGGCAAGTCAAGAAA+TGG | - | chr3.2:71117419-71117438 | MS.gene25758:intron | 40.0% | |
| ATTTGACGAAAAGGAGCTTC+AGG | - | chr3.2:71117054-71117073 | MS.gene25758:CDS | 40.0% | |
| CATGAGTTACAATAGAGAGG+TGG | + | chr3.2:71117596-71117615 | None:intergenic | 40.0% | |
| GATCATTACAGTGTGGTCAA+GGG | + | chr3.2:71117254-71117273 | None:intergenic | 40.0% | |
| GCATGCATTTGTAATGCACT+AGG | + | chr3.2:71117501-71117520 | None:intergenic | 40.0% | |
| GCATGCGATAATTACGTGTA+TGG | - | chr3.2:71117175-71117194 | MS.gene25758:intron | 40.0% | |
| GGCTAGAGCTATAATATGGT+TGG | - | chr3.2:71117404-71117423 | MS.gene25758:intron | 40.0% | |
| TGATCATTACAGTGTGGTCA+AGG | + | chr3.2:71117255-71117274 | None:intergenic | 40.0% | |
| TGCACTAGGTTGCATTGAAA+GGG | + | chr3.2:71117487-71117506 | None:intergenic | 40.0% | |
| TGCAGGCTAGAGCTATAATA+TGG | - | chr3.2:71117400-71117419 | MS.gene25758:intron | 40.0% | |
| TGCATTGAAAGGGATAGAGT+TGG | + | chr3.2:71117477-71117496 | None:intergenic | 40.0% | |
| TTACAAATGCATGCCTCACA+TGG | - | chr3.2:71117507-71117526 | MS.gene25758:intron | 40.0% | |
| TTGGCAAGTCAAGAAATGGA+TGG | - | chr3.2:71117423-71117442 | MS.gene25758:intron | 40.0% | |
| CTTGATGGAAAAGCCATGTG+AGG | + | chr3.2:71117523-71117542 | None:intergenic | 45.0% | |
| CTTGTTGCAAATCCTCGCAA+TGG | - | chr3.2:71117022-71117041 | MS.gene25758:CDS | 45.0% | |
| ! | GCTCCTTTTCGTCAAATCTG+TGG | + | chr3.2:71117051-71117070 | None:intergenic | 45.0% |
| AATCTGTGGTGTCCATTGCG+AGG | + | chr3.2:71117037-71117056 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 71116856 | 71117644 | 71116856 | ID=MS.gene25758 |
| chr3.2 | mRNA | 71116856 | 71117644 | 71116856 | ID=MS.gene25758.t1;Parent=MS.gene25758 |
| chr3.2 | exon | 71117601 | 71117644 | 71117601 | ID=MS.gene25758.t1.exon1;Parent=MS.gene25758.t1 |
| chr3.2 | CDS | 71117601 | 71117644 | 71117601 | ID=cds.MS.gene25758.t1;Parent=MS.gene25758.t1 |
| chr3.2 | exon | 71117425 | 71117506 | 71117425 | ID=MS.gene25758.t1.exon2;Parent=MS.gene25758.t1 |
| chr3.2 | CDS | 71117425 | 71117506 | 71117425 | ID=cds.MS.gene25758.t1;Parent=MS.gene25758.t1 |
| chr3.2 | exon | 71116856 | 71117095 | 71116856 | ID=MS.gene25758.t1.exon3;Parent=MS.gene25758.t1 |
| chr3.2 | CDS | 71116856 | 71117095 | 71116856 | ID=cds.MS.gene25758.t1;Parent=MS.gene25758.t1 |
| Gene Sequence |
| Protein sequence |
