AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25897

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25897.t1 MTR_3g078770 94.548 642 18 7 1 638 1 629 0.0 1241
MS.gene25897.t1 MTR_5g087790 68.372 645 162 18 2 636 1 613 0.0 833
MS.gene25897.t1 MTR_2g098470 63.399 306 98 4 41 334 27 330 1.20e-129 395
MS.gene25897.t1 MTR_3g087530 63.953 258 82 4 49 301 29 280 4.33e-112 343
MS.gene25897.t1 MTR_6g060600 38.370 503 264 9 26 520 17 481 8.10e-110 345
MS.gene25897.t1 MTR_6g460640 50.160 313 145 4 26 333 17 323 4.59e-107 338
MS.gene25897.t1 MTR_8g091370 60.232 259 97 3 46 301 16 271 1.46e-105 327
MS.gene25897.t1 MTR_3g010790 52.000 250 116 2 106 351 1 250 4.77e-89 286
MS.gene25897.t1 MTR_1g024420 44.660 103 55 1 48 148 6 108 1.75e-26 104
MS.gene25897.t1 MTR_7g095580 42.056 107 44 3 95 189 1 101 5.22e-19 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25897.t1 AT3G20770 57.034 654 236 15 3 637 1 628 0.0 683
MS.gene25897.t1 AT2G27050 58.725 596 181 18 2 572 1 556 0.0 628
MS.gene25897.t1 AT1G73730 62.541 307 93 6 43 334 29 328 2.25e-126 385
MS.gene25897.t1 AT1G73730 62.541 307 93 6 43 334 17 316 2.48e-126 384
MS.gene25897.t1 AT5G21120 46.578 453 187 13 2 436 8 423 2.74e-119 365
MS.gene25897.t1 AT5G21120 46.578 453 187 13 2 436 1 416 2.99e-119 365
MS.gene25897.t1 AT5G21120 46.578 453 187 13 2 436 1 416 2.99e-119 365
MS.gene25897.t1 AT5G65100 44.208 423 191 11 47 432 33 447 3.85e-105 330
MS.gene25897.t1 AT5G10120 57.634 262 108 1 43 301 14 275 4.33e-103 322

Find 212 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTACAAGCTTGCGAATTT+TGG 0.116142 3.2:-69538814 None:intergenic
GATCTTTCGGTAAACCAATT+TGG 0.148232 3.2:-69538695 None:intergenic
GGGTCTTCTTCATCTGATTT+TGG 0.218356 3.2:+69539314 MS.gene25897:CDS
ATCTTTCGGTAAACCAATTT+GGG 0.228960 3.2:-69538694 None:intergenic
AATAATTTCCATTTAATGTT+TGG 0.231056 3.2:+69539794 MS.gene25897:CDS
GTGGGGACCCAAACATTAAA+TGG 0.259726 3.2:-69539802 None:intergenic
CCAAGCAGACAATGCAATTC+CGG 0.279173 3.2:+69538488 MS.gene25897:CDS
CCGATGTGAGCTTGGCTATA+AGG 0.303939 3.2:-69539239 None:intergenic
AATTCAAAGCTTTGAACCTT+TGG 0.308642 3.2:-69539724 None:intergenic
AATGGCTCAGCCGGTTAATA+TGG 0.309330 3.2:+69539406 MS.gene25897:CDS
ATAATTTCCATTTAATGTTT+GGG 0.318681 3.2:+69539795 MS.gene25897:CDS
GGTCACAGGCTTTCCTTTCT+CGG 0.320948 3.2:-69538385 None:intergenic
CATGTGATGATGATTGTTAT+TGG 0.322259 3.2:-69539675 None:intergenic
GTCTGCTTGGTACTTGGATA+TGG 0.322770 3.2:-69538475 None:intergenic
ACCGGCTGAGCCATTGGTTT+CGG 0.323642 3.2:-69539398 None:intergenic
GGTGCTTCTCAAGCGATTAA+AGG 0.326570 3.2:+69538200 MS.gene25897:CDS
CGTTCCTCCACATCAAAATT+CGG 0.328270 3.2:-69539020 None:intergenic
AGGCTCAAGGGTTTGTTTAC+GGG 0.333360 3.2:+69538355 MS.gene25897:CDS
CCAAAATTCGCAAGCTTGTA+AGG 0.334057 3.2:+69538814 MS.gene25897:CDS
CAGATATTTCTTCATCAGTA+TGG 0.343697 3.2:+69539900 MS.gene25897:CDS
AAGGCTCAAGGGTTTGTTTA+CGG 0.347915 3.2:+69538354 MS.gene25897:CDS
GGCGGAGGACCTTGATCTTT+CGG 0.355023 3.2:-69538708 None:intergenic
CCTCCTCCATGGTGGCCTAC+AGG 0.364645 3.2:+69538654 MS.gene25897:CDS
TGCATTGTCTGCTTGGTACT+TGG 0.367926 3.2:-69538481 None:intergenic
GATGAAGAAGACCCTCTATA+TGG 0.368108 3.2:-69539305 None:intergenic
TTGATTAACGATTGCAAGCC+AGG 0.377242 3.2:-69538883 None:intergenic
AAAGGTCCAGCTGCGACAAA+TGG 0.377442 3.2:-69538948 None:intergenic
CCGGAATTGCATTGTCTGCT+TGG 0.381090 3.2:-69538488 None:intergenic
GTCACAGGCTTTCCTTTCTC+GGG 0.386577 3.2:-69538384 None:intergenic
GAGGAGTGGTGGCCCCAAAT+TGG 0.394929 3.2:+69538681 MS.gene25897:CDS
TTCTTCATCAATCCAACATT+GGG 0.399620 3.2:+69539057 MS.gene25897:CDS
GAGGAGGATGTGGAGGGATA+AGG 0.401050 3.2:+69538179 MS.gene25897:CDS
CCACCTTCCATCGATATAAC+TGG 0.402818 3.2:+69539431 MS.gene25897:CDS
CATATCTCCCTTGAACTCAT+AGG 0.404451 3.2:-69539840 None:intergenic
AGATCATGAGGTTTCTTGTA+AGG 0.405253 3.2:-69538729 None:intergenic
ATTGCGAAAGTCTGCTGAAA+TGG 0.405530 3.2:-69539110 None:intergenic
TTTCCTCCTTGCCTGTTCCT+GGG 0.410461 3.2:-69538271 None:intergenic
ATCTCATTAGCATGAAAACT+TGG 0.413498 3.2:-69539341 None:intergenic
AAGGTCCAGCTGCGACAAAT+GGG 0.416033 3.2:-69538947 None:intergenic
CAAGCAGACAATGCAATTCC+GGG 0.417322 3.2:+69538489 MS.gene25897:CDS
TTTGTCGCAGCTGGACCTTT+CGG 0.419285 3.2:+69538951 MS.gene25897:CDS
TTGCCTCTGCTTGGCCGCAT+CGG 0.422356 3.2:-69538247 None:intergenic
TCGCCTCAGGTTCAGTTAGC+CGG 0.423673 3.2:-69538107 None:intergenic
GATTGATGAAGAAGATTCTC+AGG 0.425025 3.2:-69539047 None:intergenic
CATACTGATGAAGAAATATC+TGG 0.429090 3.2:-69539899 None:intergenic
TCAATCCAACATTGGGATGA+TGG 0.429901 3.2:+69539064 MS.gene25897:CDS
GCCGCATCGGATCCTTCCTT+CGG 0.430698 3.2:-69538234 None:intergenic
GATCATGAGATGGCTCTATC+TGG 0.431769 3.2:+69538030 MS.gene25897:CDS
GGCAACATGAATTTAACTTC+TGG 0.433244 3.2:+69539524 MS.gene25897:CDS
CCCTCAGAGGCGGTTTCCAT+TGG 0.434105 3.2:+69538620 MS.gene25897:CDS
TTCCTGGGATTGCCTCTGCT+TGG 0.439634 3.2:-69538256 None:intergenic
GCAGCTCTTGCAAGGTGTGA+GGG 0.439936 3.2:-69538545 None:intergenic
CCGCGAATGGTGGAAGGATA+AGG 0.449952 3.2:+69538425 MS.gene25897:CDS
GCAGAGGCAATCCCAGGAAC+AGG 0.451574 3.2:+69538260 MS.gene25897:CDS
CTCCTCCATGGTGGCCTACA+GGG 0.453443 3.2:+69538655 MS.gene25897:CDS
CCTTATCCTTCCACCATTCG+CGG 0.453482 3.2:-69538425 None:intergenic
GTGAGGAAGCGGAAGATCTC+TGG 0.455579 3.2:+69539164 MS.gene25897:CDS
TACGGGATCATTCCCGAGAA+AGG 0.459725 3.2:+69538372 MS.gene25897:CDS
GAAGGTGGAACCATATTAAC+CGG 0.463028 3.2:-69539416 None:intergenic
CTTCTTCATCAATCCAACAT+TGG 0.463424 3.2:+69539056 MS.gene25897:CDS
AGCAGCATGAGAATTACTTC+AGG 0.465101 3.2:+69539603 MS.gene25897:CDS
TCAGCAGACTTTCGCAATGA+AGG 0.468808 3.2:+69539115 MS.gene25897:CDS
CAATTGAGTTGTCCATATAG+AGG 0.471657 3.2:+69539293 MS.gene25897:CDS
ATGATTGTTATTGGACATGT+TGG 0.474263 3.2:-69539666 None:intergenic
TGCAGCTCTTGCAAGGTGTG+AGG 0.478278 3.2:-69538546 None:intergenic
GAGAAAGGAAAGCCTGTGAC+CGG 0.478282 3.2:+69538387 MS.gene25897:CDS
ATATTAACCGGCTGAGCCAT+TGG 0.478420 3.2:-69539404 None:intergenic
GTATCCCTAGAAGCCCTGTC+TGG 0.478900 3.2:-69539266 None:intergenic
TCACACCTTGCAAGAGCTGC+AGG 0.479872 3.2:+69538548 MS.gene25897:CDS
CGCCTCAGGTTCAGTTAGCC+GGG 0.481589 3.2:-69538106 None:intergenic
GATGGCTCTATCTGGTGATC+TGG 0.482695 3.2:+69538038 MS.gene25897:CDS
TCTTGCAAGGTGTGAGGGGT+TGG 0.482958 3.2:-69538540 None:intergenic
CACATGTTTGCAAGAGATGA+AGG 0.486109 3.2:+69539692 MS.gene25897:CDS
GTCTATGATTCAGACATTCA+AGG 0.486371 3.2:+69539503 MS.gene25897:CDS
GCTCCCCAACCCCAAGTTGA+GGG 0.486678 3.2:+69538072 MS.gene25897:CDS
ATTGGTTTACCGAAAGATCA+AGG 0.487239 3.2:+69538699 MS.gene25897:CDS
TGCTCCCCAACCCCAAGTTG+AGG 0.487998 3.2:+69538071 MS.gene25897:CDS
TCCTTCCCTGTAGGCCACCA+TGG 0.488917 3.2:-69538660 None:intergenic
CTCTGGTGAATTTAACATGA+TGG 0.489439 3.2:+69539181 MS.gene25897:CDS
GAGAATTACTTCAGGGGTCA+AGG 0.490089 3.2:+69539611 MS.gene25897:CDS
CATGCCTTCTTCAGATCATG+AGG 0.490321 3.2:-69538741 None:intergenic
GGCCAAGCAGAGGCAATCCC+AGG 0.494536 3.2:+69538254 MS.gene25897:CDS
TTTCTGAAGATGGTGAGAAA+AGG 0.494881 3.2:+69539462 MS.gene25897:CDS
CCAATGGAAACCGCCTCTGA+GGG 0.495394 3.2:-69538620 None:intergenic
CCGAGCTTGAGAGGAGGATG+TGG 0.496132 3.2:+69538169 MS.gene25897:CDS
AATGGTGGAAGGATAAGGTT+AGG 0.496339 3.2:+69538430 MS.gene25897:CDS
GTGTGAGGGGTTGGACCAAT+CGG 0.497518 3.2:-69538531 None:intergenic
GCAATTCCGGGGAAGAATGA+TGG 0.501691 3.2:+69538501 MS.gene25897:CDS
TTTGACATCCTATGAGTTCA+AGG 0.502925 3.2:+69539832 MS.gene25897:CDS
CGGATAATCTCCGCGAATGG+TGG 0.504192 3.2:+69538415 MS.gene25897:CDS
ACCGAAACCAATGGCTCAGC+CGG 0.505945 3.2:+69539397 MS.gene25897:CDS
ATTGGTTTCGGTTGAACGAA+TGG 0.506668 3.2:-69539386 None:intergenic
TCTTCGTCAACCATCGCCTC+AGG 0.506692 3.2:-69538120 None:intergenic
GGACTTGGAGTTTCTGAAGA+TGG 0.507451 3.2:+69539452 MS.gene25897:CDS
CTGAAGAAGGCATGGAAGGT+TGG 0.507865 3.2:+69538750 MS.gene25897:CDS
TTATCCGATGCCCCGGTCAC+AGG 0.510724 3.2:-69538399 None:intergenic
TCTCTCCATCATCCCAATGT+TGG 0.511441 3.2:-69539069 None:intergenic
AGAAGGGTGTTCCTCCTCCA+TGG 0.516637 3.2:+69538643 MS.gene25897:CDS
ATGGAGGTTTGCAAGGCTCA+AGG 0.517295 3.2:+69538342 MS.gene25897:CDS
GGAAGAGGCTGTGACCAACT+TGG 0.519473 3.2:+69539136 MS.gene25897:CDS
AGGATAAAGCTCCCGCGCTA+AGG 0.520455 3.2:-69538913 None:intergenic
GAGCTGCAGGACACAACCTT+GGG 0.520799 3.2:+69538561 MS.gene25897:CDS
ACTTGGATTTCGTGAGGAAG+CGG 0.521923 3.2:+69539153 MS.gene25897:CDS
AAAGCCTGTGACCGGGGCAT+CGG 0.525234 3.2:+69538395 MS.gene25897:CDS
GGACCTTTCGGCATCAATGA+AGG 0.525234 3.2:+69538963 MS.gene25897:CDS
AGAAAGGAAAGCCTGTGACC+GGG 0.526532 3.2:+69538388 MS.gene25897:CDS
GGCCTACAGGGAAGGAGGAG+TGG 0.527829 3.2:+69538667 MS.gene25897:CDS
TGTGTCCTGCAGCTCTTGCA+AGG 0.529578 3.2:-69538553 None:intergenic
TGATTGTGATTGTTCTCAAA+GGG 0.531892 3.2:-69539749 None:intergenic
GAGGCGGTTTCCATTGGAGA+AGG 0.533081 3.2:+69538626 MS.gene25897:CDS
CTGGAAAACCGATGTGAGCT+TGG 0.533885 3.2:-69539247 None:intergenic
TTCGGTTGAACGAATGGCTG+AGG 0.536276 3.2:-69539380 None:intergenic
CGGCTAAGGAAAGTGCAACC+TGG 0.537233 3.2:+69538865 MS.gene25897:CDS
TCAAGATAAAATGACGGCTA+AGG 0.537615 3.2:+69538851 MS.gene25897:CDS
TGCTGAATGCTGCTAGCTTG+CGG 0.538137 3.2:-69539581 None:intergenic
TGAGGTTTCTTGTAAGGCGG+AGG 0.540347 3.2:-69538723 None:intergenic
GAAGTACATGCTGAAAATGA+TGG 0.543075 3.2:+69538323 MS.gene25897:CDS
TGGTACTTGGATATGGCAGC+AGG 0.543745 3.2:-69538468 None:intergenic
GAAAGGAAAGCCTGTGACCG+GGG 0.544653 3.2:+69538389 MS.gene25897:CDS
GACTTTCGCAATGAAGGAAG+AGG 0.546905 3.2:+69539121 MS.gene25897:CDS
TTGCCTCCATCATTCTTCCC+CGG 0.547718 3.2:-69538507 None:intergenic
CTCATGATCTGAAGAAGGCA+TGG 0.547916 3.2:+69538742 MS.gene25897:CDS
TTGATGTTGCCGAGCTTGAG+AGG 0.548157 3.2:+69538160 MS.gene25897:CDS
GCAATGAGTATGATGTCGAT+GGG 0.548446 3.2:+69538985 MS.gene25897:CDS
GAAACCTCATGATCTGAAGA+AGG 0.550430 3.2:+69538737 MS.gene25897:CDS
AGAGCTGCAGGACACAACCT+TGG 0.551353 3.2:+69538560 MS.gene25897:CDS
GCAGCATGAGAATTACTTCA+GGG 0.554925 3.2:+69539604 MS.gene25897:CDS
TAATCTCCGCGAATGGTGGA+AGG 0.556888 3.2:+69538419 MS.gene25897:CDS
GTGATTGTGATTGTTCTCAA+AGG 0.561089 3.2:-69539750 None:intergenic
AGCTTGAGAGGAGGATGTGG+AGG 0.561978 3.2:+69538172 MS.gene25897:CDS
TGCAGATAAGAGAGAACCCA+AGG 0.562468 3.2:-69538577 None:intergenic
TCCAAGTCCAGTTATATCGA+TGG 0.562663 3.2:-69539438 None:intergenic
CACCACTCCTCCTTCCCTGT+AGG 0.563392 3.2:-69538669 None:intergenic
CCTGTAGGCCACCATGGAGG+AGG 0.564770 3.2:-69538654 None:intergenic
GGCAATCCCAGGAACAGGCA+AGG 0.565695 3.2:+69538265 MS.gene25897:CDS
TCCAATGGAAACCGCCTCTG+AGG 0.567003 3.2:-69538621 None:intergenic
CAGCTCTTGCAAGGTGTGAG+GGG 0.568677 3.2:-69538544 None:intergenic
TGGAGGTTTGCAAGGCTCAA+GGG 0.571141 3.2:+69538343 MS.gene25897:CDS
TGATCTGAAGAAGGCATGGA+AGG 0.572466 3.2:+69538746 MS.gene25897:CDS
CTACAGGGAAGGAGGAGTGG+TGG 0.573928 3.2:+69538670 MS.gene25897:CDS
TATGCATGGAGTAGGCATCA+TGG 0.573987 3.2:+69539859 MS.gene25897:CDS
ATGAAGGTCAATTTGACCAA+AGG 0.575029 3.2:+69539708 MS.gene25897:CDS
TCCATCGATATAACTGGACT+TGG 0.575840 3.2:+69539437 MS.gene25897:CDS
GGTCCAGCTGCGACAAATGG+GGG 0.576693 3.2:-69538945 None:intergenic
AAGGGAGATATGCATGGAGT+AGG 0.577953 3.2:+69539851 MS.gene25897:CDS
TTACTTCAGGGGTCAAGGAA+TGG 0.577986 3.2:+69539616 MS.gene25897:CDS
GATCCGATGCGGCCAAGCAG+AGG 0.578212 3.2:+69538244 MS.gene25897:CDS
GGAGGAACACCCTTCTCCAA+TGG 0.579400 3.2:-69538636 None:intergenic
AGGTCCAGCTGCGACAAATG+GGG 0.579864 3.2:-69538946 None:intergenic
GCTAACTGAACCTGAGGCGA+TGG 0.580269 3.2:+69538110 MS.gene25897:CDS
AGGATGTCAAATCACAATGT+GGG 0.580845 3.2:-69539820 None:intergenic
CTCCCCAACCCCAAGTTGAG+GGG 0.581721 3.2:+69538073 MS.gene25897:CDS
TCCATGGTGGCCTACAGGGA+AGG 0.582532 3.2:+69538659 MS.gene25897:CDS
CTTCCTCACGAAATCCAAGT+TGG 0.583208 3.2:-69539150 None:intergenic
AATGATGGAGGCAATCCGAT+TGG 0.584431 3.2:+69538516 MS.gene25897:CDS
AATGGAAACCGCCTCTGAGG+GGG 0.586396 3.2:-69538618 None:intergenic
AATGGCTGAGGATATATGAC+TGG 0.587547 3.2:-69539368 None:intergenic
GAGTTCAAGGGAGATATGCA+TGG 0.587678 3.2:+69539845 MS.gene25897:CDS
TCAAGAGGAGGCCTTAGCGC+GGG 0.589414 3.2:+69538902 MS.gene25897:CDS
TTGACATCCTATGAGTTCAA+GGG 0.590024 3.2:+69539833 MS.gene25897:CDS
AATTGAGTTGTCCATATAGA+GGG 0.591180 3.2:+69539294 MS.gene25897:CDS
GAAAATGATGGAGGTTTGCA+AGG 0.591397 3.2:+69538335 MS.gene25897:CDS
AAGATGTCAAGGGCTCAAGA+TGG 0.596334 3.2:+69538294 MS.gene25897:CDS
GGTCAAGGAATGGTGATGGA+AGG 0.597963 3.2:+69539626 MS.gene25897:CDS
CAGGGGTCAAGGAATGGTGA+TGG 0.599899 3.2:+69539622 MS.gene25897:CDS
TTGCCTTCATTGATGCCGAA+AGG 0.600278 3.2:-69538966 None:intergenic
GAAGATGGTGAGAAAAGGAT+TGG 0.601828 3.2:+69539467 MS.gene25897:CDS
GAGCAAGTCAAACCGAAGGA+AGG 0.602432 3.2:+69538222 MS.gene25897:CDS
AAGGTTAGGTTTGACCGCAA+CGG 0.602662 3.2:+69538444 MS.gene25897:CDS
CCAGTTATATCGATGGAAGG+TGG 0.610149 3.2:-69539431 None:intergenic
TCCCCAACCCCAAGTTGAGG+GGG 0.613156 3.2:+69538074 MS.gene25897:CDS
CGCCCGGCTAACTGAACCTG+AGG 0.614118 3.2:+69538104 MS.gene25897:CDS
CCACATCCTCCTCTCAAGCT+CGG 0.615219 3.2:-69538169 None:intergenic
GGCAATGAGTATGATGTCGA+TGG 0.618237 3.2:+69538984 MS.gene25897:CDS
AGGCGGTTTCCATTGGAGAA+GGG 0.619284 3.2:+69538627 MS.gene25897:CDS
CATCGGATAATCTCCGCGAA+TGG 0.620613 3.2:+69538412 MS.gene25897:CDS
TCTTTCGGTAAACCAATTTG+GGG 0.621082 3.2:-69538693 None:intergenic
ATGGTGGCCTACAGGGAAGG+AGG 0.622832 3.2:+69538662 MS.gene25897:CDS
GCTTGCAATCGTTAATCAAG+AGG 0.626115 3.2:+69538887 MS.gene25897:CDS
GCGGAGATTATCCGATGCCC+CGG 0.628966 3.2:-69538406 None:intergenic
TAGGATGTCAAATCACAATG+TGG 0.629786 3.2:-69539821 None:intergenic
TCATGAGGTTTCTTGTAAGG+CGG 0.630329 3.2:-69538726 None:intergenic
GATGATGTTTGATCATGAGA+TGG 0.631968 3.2:+69538020 MS.gene25897:CDS
ATCAAGAGGAGGCCTTAGCG+CGG 0.637665 3.2:+69538901 MS.gene25897:CDS
GCTTGAGAGGAGGATGTGGA+GGG 0.637705 3.2:+69538173 MS.gene25897:CDS
TGACCAACTTGGATTTCGTG+AGG 0.639847 3.2:+69539147 MS.gene25897:CDS
CCTTATAGCCAAGCTCACAT+CGG 0.641231 3.2:+69539239 MS.gene25897:CDS
ATTCGTTCAACCGAAACCAA+TGG 0.641894 3.2:+69539388 MS.gene25897:CDS
AGTCCAGTTATATCGATGGA+AGG 0.642356 3.2:-69539434 None:intergenic
TGCAATCGTTAATCAAGAGG+AGG 0.642676 3.2:+69538890 MS.gene25897:CDS
ATGCCTTCAAGATAAAATGA+CGG 0.643938 3.2:+69538845 MS.gene25897:CDS
GGATGTCAAATCACAATGTG+GGG 0.645049 3.2:-69539819 None:intergenic
AAAGGAGCAAGTCAAACCGA+AGG 0.648111 3.2:+69538218 MS.gene25897:CDS
GATGGTGAGAAAAGGATTGG+TGG 0.649012 3.2:+69539470 MS.gene25897:CDS
ATATGGCAGCAGGACCGTTG+CGG 0.650469 3.2:-69538458 None:intergenic
TATAAGGACATGTTGAGTGT+TGG 0.651109 3.2:-69539223 None:intergenic
AGGGTGTTCCTCCTCCATGG+TGG 0.653236 3.2:+69538646 MS.gene25897:CDS
CAATGGAAACCGCCTCTGAG+GGG 0.659298 3.2:-69538619 None:intergenic
GTCCAGCTGCGACAAATGGG+GGG 0.662952 3.2:-69538944 None:intergenic
CAATGAGTATGATGTCGATG+GGG 0.670997 3.2:+69538986 MS.gene25897:CDS
AATGAGTATGATGTCGATGG+GGG 0.671000 3.2:+69538987 MS.gene25897:CDS
TTCCCTGTAGGCCACCATGG+AGG 0.671351 3.2:-69538657 None:intergenic
AATCCCAGGAACAGGCAAGG+AGG 0.672658 3.2:+69538268 MS.gene25897:CDS
ATGTTGCCGAGCTTGAGAGG+AGG 0.673867 3.2:+69538163 MS.gene25897:CDS
ATTCCGGGGAAGAATGATGG+AGG 0.679869 3.2:+69538504 MS.gene25897:CDS
AAGCAGACAATGCAATTCCG+GGG 0.691771 3.2:+69538490 MS.gene25897:CDS
CAGCATGAGAATTACTTCAG+GGG 0.700026 3.2:+69539605 MS.gene25897:CDS
CTCAGGTTCAGTTAGCCGGG+CGG 0.704152 3.2:-69538103 None:intergenic
CTGAATCATAGACAGTCATG+AGG 0.706334 3.2:-69539493 None:intergenic
TGGCAGCCACTTAACCGCAG+CGG 0.706818 3.2:+69539544 MS.gene25897:CDS
ACCGAAGGAAGGATCCGATG+CGG 0.718950 3.2:+69538233 MS.gene25897:CDS
GTACATGCTGAAAATGATGG+AGG 0.724912 3.2:+69538326 MS.gene25897:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTTCCATTTAATGTT+TGG + chr3.2:69539794-69539813 MS.gene25897:CDS 15.0%
!! ATAATTTCCATTTAATGTTT+GGG + chr3.2:69539795-69539814 MS.gene25897:CDS 15.0%
ATCTCATTAGCATGAAAACT+TGG - chr3.2:69539344-69539363 None:intergenic 30.0%
ATCTTTCGGTAAACCAATTT+GGG - chr3.2:69538697-69538716 None:intergenic 30.0%
ATGCCTTCAAGATAAAATGA+CGG + chr3.2:69538845-69538864 MS.gene25897:CDS 30.0%
CAGATATTTCTTCATCAGTA+TGG + chr3.2:69539900-69539919 MS.gene25897:CDS 30.0%
CATACTGATGAAGAAATATC+TGG - chr3.2:69539902-69539921 None:intergenic 30.0%
TGATTGTGATTGTTCTCAAA+GGG - chr3.2:69539752-69539771 None:intergenic 30.0%
TTCTTCATCAATCCAACATT+GGG + chr3.2:69539057-69539076 MS.gene25897:CDS 30.0%
! AATTGAGTTGTCCATATAGA+GGG + chr3.2:69539294-69539313 MS.gene25897:CDS 30.0%
! ATGATTGTTATTGGACATGT+TGG - chr3.2:69539669-69539688 None:intergenic 30.0%
! CATGTGATGATGATTGTTAT+TGG - chr3.2:69539678-69539697 None:intergenic 30.0%
! TGTTGGCAAGTGAAAATTTT+AGG - chr3.2:69539209-69539228 None:intergenic 30.0%
!! AATTCAAAGCTTTGAACCTT+TGG - chr3.2:69539727-69539746 None:intergenic 30.0%
!!! CATTTTATCTTGAAGGCATT+TGG - chr3.2:69538844-69538863 None:intergenic 30.0%
AAGGAGGAAAAAGATGTCAA+GGG + chr3.2:69538284-69538303 MS.gene25897:CDS 35.0%
AGATCATGAGGTTTCTTGTA+AGG - chr3.2:69538732-69538751 None:intergenic 35.0%
AGGATGTCAAATCACAATGT+GGG - chr3.2:69539823-69539842 None:intergenic 35.0%
ATGAAGGTCAATTTGACCAA+AGG + chr3.2:69539708-69539727 MS.gene25897:CDS 35.0%
CTCTGGTGAATTTAACATGA+TGG + chr3.2:69539181-69539200 MS.gene25897:CDS 35.0%
CTTCTTCATCAATCCAACAT+TGG + chr3.2:69539056-69539075 MS.gene25897:CDS 35.0%
GAAGTACATGCTGAAAATGA+TGG + chr3.2:69538323-69538342 MS.gene25897:CDS 35.0%
GATCTTTCGGTAAACCAATT+TGG - chr3.2:69538698-69538717 None:intergenic 35.0%
GATGATGTTTGATCATGAGA+TGG + chr3.2:69538020-69538039 MS.gene25897:CDS 35.0%
GATTGATGAAGAAGATTCTC+AGG - chr3.2:69539050-69539069 None:intergenic 35.0%
GTCTATGATTCAGACATTCA+AGG + chr3.2:69539503-69539522 MS.gene25897:CDS 35.0%
GTGATTGTGATTGTTCTCAA+AGG - chr3.2:69539753-69539772 None:intergenic 35.0%
TAGGATGTCAAATCACAATG+TGG - chr3.2:69539824-69539843 None:intergenic 35.0%
TATAAGGACATGTTGAGTGT+TGG - chr3.2:69539226-69539245 None:intergenic 35.0%
TCAAGATAAAATGACGGCTA+AGG + chr3.2:69538851-69538870 MS.gene25897:CDS 35.0%
TCTTTCGGTAAACCAATTTG+GGG - chr3.2:69538696-69538715 None:intergenic 35.0%
TTGACATCCTATGAGTTCAA+GGG + chr3.2:69539833-69539852 MS.gene25897:CDS 35.0%
TTTGACATCCTATGAGTTCA+AGG + chr3.2:69539832-69539851 MS.gene25897:CDS 35.0%
! ATTGGTTTACCGAAAGATCA+AGG + chr3.2:69538699-69538718 MS.gene25897:CDS 35.0%
! CAATTGAGTTGTCCATATAG+AGG + chr3.2:69539293-69539312 MS.gene25897:CDS 35.0%
! GGCAACATGAATTTAACTTC+TGG + chr3.2:69539524-69539543 MS.gene25897:CDS 35.0%
! TCTTCTTCATCTGATTTTGG+TGG + chr3.2:69539317-69539336 MS.gene25897:CDS 35.0%
! TTTCTGAAGATGGTGAGAAA+AGG + chr3.2:69539462-69539481 MS.gene25897:CDS 35.0%
!! TAGCCGTCATTTTATCTTGA+AGG - chr3.2:69538851-69538870 None:intergenic 35.0%
AATGAGTATGATGTCGATGG+GGG + chr3.2:69538987-69539006 MS.gene25897:CDS 40.0%
AATGGCTGAGGATATATGAC+TGG - chr3.2:69539371-69539390 None:intergenic 40.0%
ACAAATGGGGGGATATAATC+AGG - chr3.2:69538936-69538955 None:intergenic 40.0%
AGCAGCATGAGAATTACTTC+AGG + chr3.2:69539603-69539622 MS.gene25897:CDS 40.0%
AGTCCAGTTATATCGATGGA+AGG - chr3.2:69539437-69539456 None:intergenic 40.0%
ATTCGTTCAACCGAAACCAA+TGG + chr3.2:69539388-69539407 MS.gene25897:CDS 40.0%
ATTGCGAAAGTCTGCTGAAA+TGG - chr3.2:69539113-69539132 None:intergenic 40.0%
CAAGGAGGAAAAAGATGTCA+AGG + chr3.2:69538283-69538302 MS.gene25897:CDS 40.0%
CAATGAGTATGATGTCGATG+GGG + chr3.2:69538986-69539005 MS.gene25897:CDS 40.0%
CACATGTTTGCAAGAGATGA+AGG + chr3.2:69539692-69539711 MS.gene25897:CDS 40.0%
CAGCATGAGAATTACTTCAG+GGG + chr3.2:69539605-69539624 MS.gene25897:CDS 40.0%
CATATCTCCCTTGAACTCAT+AGG - chr3.2:69539843-69539862 None:intergenic 40.0%
CCAAAATTCGCAAGCTTGTA+AGG + chr3.2:69538814-69538833 MS.gene25897:CDS 40.0%
CGTTCCTCCACATCAAAATT+CGG - chr3.2:69539023-69539042 None:intergenic 40.0%
CTGAATCATAGACAGTCATG+AGG - chr3.2:69539496-69539515 None:intergenic 40.0%
GAAACCTCATGATCTGAAGA+AGG + chr3.2:69538737-69538756 MS.gene25897:CDS 40.0%
GAAGATGGTGAGAAAAGGAT+TGG + chr3.2:69539467-69539486 MS.gene25897:CDS 40.0%
GAAGGTGGAACCATATTAAC+CGG - chr3.2:69539419-69539438 None:intergenic 40.0%
GATGAAGAAGACCCTCTATA+TGG - chr3.2:69539308-69539327 None:intergenic 40.0%
GCAATGAGTATGATGTCGAT+GGG + chr3.2:69538985-69539004 MS.gene25897:CDS 40.0%
GCAGCATGAGAATTACTTCA+GGG + chr3.2:69539604-69539623 MS.gene25897:CDS 40.0%
GCTTGCAATCGTTAATCAAG+AGG + chr3.2:69538887-69538906 MS.gene25897:CDS 40.0%
GGATGTCAAATCACAATGTG+GGG - chr3.2:69539822-69539841 None:intergenic 40.0%
GTACATGCTGAAAATGATGG+AGG + chr3.2:69538326-69538345 MS.gene25897:CDS 40.0%
TCAATCCAACATTGGGATGA+TGG + chr3.2:69539064-69539083 MS.gene25897:CDS 40.0%
TCATGAGGTTTCTTGTAAGG+CGG - chr3.2:69538729-69538748 None:intergenic 40.0%
TCCAAGTCCAGTTATATCGA+TGG - chr3.2:69539441-69539460 None:intergenic 40.0%
TCCATCGATATAACTGGACT+TGG + chr3.2:69539437-69539456 MS.gene25897:CDS 40.0%
TGCAATCGTTAATCAAGAGG+AGG + chr3.2:69538890-69538909 MS.gene25897:CDS 40.0%
TTGATTAACGATTGCAAGCC+AGG - chr3.2:69538886-69538905 None:intergenic 40.0%
! AAGGCTCAAGGGTTTGTTTA+CGG + chr3.2:69538354-69538373 MS.gene25897:CDS 40.0%
! AATGGTGGAAGGATAAGGTT+AGG + chr3.2:69538430-69538449 MS.gene25897:CDS 40.0%
! ATTGGTTTCGGTTGAACGAA+TGG - chr3.2:69539389-69539408 None:intergenic 40.0%
! CCTTACAAGCTTGCGAATTT+TGG - chr3.2:69538817-69538836 None:intergenic 40.0%
! GAAAATGATGGAGGTTTGCA+AGG + chr3.2:69538335-69538354 MS.gene25897:CDS 40.0%
! GGGTCTTCTTCATCTGATTT+TGG + chr3.2:69539314-69539333 MS.gene25897:CDS 40.0%
! TAAGATGTGATTTTCCGCTG+CGG - chr3.2:69539561-69539580 None:intergenic 40.0%
!! CGAATTTTGATGTGGAGGAA+CGG + chr3.2:69539021-69539040 MS.gene25897:CDS 40.0%
!! CTTGCGAATTTTGGCAATGT+CGG - chr3.2:69538808-69538827 None:intergenic 40.0%
!! TTGCGAATTTTGGCAATGTC+GGG - chr3.2:69538807-69538826 None:intergenic 40.0%
AAAGGAGCAAGTCAAACCGA+AGG + chr3.2:69538218-69538237 MS.gene25897:CDS 45.0%
AAGATGTCAAGGGCTCAAGA+TGG + chr3.2:69538294-69538313 MS.gene25897:CDS 45.0%
AAGCAGACAATGCAATTCCG+GGG + chr3.2:69538490-69538509 MS.gene25897:CDS 45.0%
AAGGGAGATATGCATGGAGT+AGG + chr3.2:69539851-69539870 MS.gene25897:CDS 45.0%
AAGGTTAGGTTTGACCGCAA+CGG + chr3.2:69538444-69538463 MS.gene25897:CDS 45.0%
AATGGCTCAGCCGGTTAATA+TGG + chr3.2:69539406-69539425 MS.gene25897:CDS 45.0%
ACTTGGATTTCGTGAGGAAG+CGG + chr3.2:69539153-69539172 MS.gene25897:CDS 45.0%
ATATTAACCGGCTGAGCCAT+TGG - chr3.2:69539407-69539426 None:intergenic 45.0%
CAAGCAGACAATGCAATTCC+GGG + chr3.2:69538489-69538508 MS.gene25897:CDS 45.0%
CATGCCTTCTTCAGATCATG+AGG - chr3.2:69538744-69538763 None:intergenic 45.0%
CCAAGCAGACAATGCAATTC+CGG + chr3.2:69538488-69538507 MS.gene25897:CDS 45.0%
CCACCTTCCATCGATATAAC+TGG + chr3.2:69539431-69539450 MS.gene25897:CDS 45.0%
CCAGTTATATCGATGGAAGG+TGG - chr3.2:69539434-69539453 None:intergenic 45.0%
CCTTATAGCCAAGCTCACAT+CGG + chr3.2:69539239-69539258 MS.gene25897:CDS 45.0%
CTCATGATCTGAAGAAGGCA+TGG + chr3.2:69538742-69538761 MS.gene25897:CDS 45.0%
CTTCCTCACGAAATCCAAGT+TGG - chr3.2:69539153-69539172 None:intergenic 45.0%
GACTTTCGCAATGAAGGAAG+AGG + chr3.2:69539121-69539140 MS.gene25897:CDS 45.0%
GAGAATTACTTCAGGGGTCA+AGG + chr3.2:69539611-69539630 MS.gene25897:CDS 45.0%
GAGTTCAAGGGAGATATGCA+TGG + chr3.2:69539845-69539864 MS.gene25897:CDS 45.0%
GATGGTGAGAAAAGGATTGG+TGG + chr3.2:69539470-69539489 MS.gene25897:CDS 45.0%
GGCAATGAGTATGATGTCGA+TGG + chr3.2:69538984-69539003 MS.gene25897:CDS 45.0%
GGTAATATCCCCCTCAACTT+GGG - chr3.2:69538085-69538104 None:intergenic 45.0%
GGTGCTTCTCAAGCGATTAA+AGG + chr3.2:69538200-69538219 MS.gene25897:CDS 45.0%
GTAATATCCCCCTCAACTTG+GGG - chr3.2:69538084-69538103 None:intergenic 45.0%
GTCTGCTTGGTACTTGGATA+TGG - chr3.2:69538478-69538497 None:intergenic 45.0%
GTGGGGACCCAAACATTAAA+TGG - chr3.2:69539805-69539824 None:intergenic 45.0%
TATGCATGGAGTAGGCATCA+TGG + chr3.2:69539859-69539878 MS.gene25897:CDS 45.0%
TCAGCAGACTTTCGCAATGA+AGG + chr3.2:69539115-69539134 MS.gene25897:CDS 45.0%
TCTCTCCATCATCCCAATGT+TGG - chr3.2:69539072-69539091 None:intergenic 45.0%
TGACCAACTTGGATTTCGTG+AGG + chr3.2:69539147-69539166 MS.gene25897:CDS 45.0%
TGCAGATAAGAGAGAACCCA+AGG - chr3.2:69538580-69538599 None:intergenic 45.0%
TGCATTGTCTGCTTGGTACT+TGG - chr3.2:69538484-69538503 None:intergenic 45.0%
TTACTTCAGGGGTCAAGGAA+TGG + chr3.2:69539616-69539635 MS.gene25897:CDS 45.0%
TTGCCTTCATTGATGCCGAA+AGG - chr3.2:69538969-69538988 None:intergenic 45.0%
! AATGATGGAGGCAATCCGAT+TGG + chr3.2:69538516-69538535 MS.gene25897:CDS 45.0%
! AGGCTCAAGGGTTTGTTTAC+GGG + chr3.2:69538355-69538374 MS.gene25897:CDS 45.0%
! GATCATGAGATGGCTCTATC+TGG + chr3.2:69538030-69538049 MS.gene25897:CDS 45.0%
! GGACTTGGAGTTTCTGAAGA+TGG + chr3.2:69539452-69539471 MS.gene25897:CDS 45.0%
! TGATCTGAAGAAGGCATGGA+AGG + chr3.2:69538746-69538765 MS.gene25897:CDS 45.0%
! TTTTCCAGACAGGGCTTCTA+GGG + chr3.2:69539262-69539281 MS.gene25897:CDS 45.0%
!! AGAGGAGCCGAATTTTGATG+TGG + chr3.2:69539013-69539032 MS.gene25897:CDS 45.0%
!!! TCACATCGGTTTTCCAGACA+GGG + chr3.2:69539253-69539272 MS.gene25897:CDS 45.0%
AAAGGTCCAGCTGCGACAAA+TGG - chr3.2:69538951-69538970 None:intergenic 50.0%
AAGGTCCAGCTGCGACAAAT+GGG - chr3.2:69538950-69538969 None:intergenic 50.0%
AGAAAGGAAAGCCTGTGACC+GGG + chr3.2:69538388-69538407 MS.gene25897:CDS 50.0%
AGGCGGTTTCCATTGGAGAA+GGG + chr3.2:69538627-69538646 MS.gene25897:CDS 50.0%
ATGGAGGTTTGCAAGGCTCA+AGG + chr3.2:69538342-69538361 MS.gene25897:CDS 50.0%
ATTCCGGGGAAGAATGATGG+AGG + chr3.2:69538504-69538523 MS.gene25897:CDS 50.0%
CATCGGATAATCTCCGCGAA+TGG + chr3.2:69538412-69538431 MS.gene25897:CDS 50.0%
CCGATGTGAGCTTGGCTATA+AGG - chr3.2:69539242-69539261 None:intergenic 50.0%
CCGGAATTGCATTGTCTGCT+TGG - chr3.2:69538491-69538510 None:intergenic 50.0%
CCTTATCCTTCCACCATTCG+CGG - chr3.2:69538428-69538447 None:intergenic 50.0%
CGGTAATATCCCCCTCAACT+TGG - chr3.2:69538086-69538105 None:intergenic 50.0%
CTGGAAAACCGATGTGAGCT+TGG - chr3.2:69539250-69539269 None:intergenic 50.0%
GAGAAAGGAAAGCCTGTGAC+CGG + chr3.2:69538387-69538406 MS.gene25897:CDS 50.0%
GAGCAAGTCAAACCGAAGGA+AGG + chr3.2:69538222-69538241 MS.gene25897:CDS 50.0%
GCAATTCCGGGGAAGAATGA+TGG + chr3.2:69538501-69538520 MS.gene25897:CDS 50.0%
GGACCTTTCGGCATCAATGA+AGG + chr3.2:69538963-69538982 MS.gene25897:CDS 50.0%
GGTCACAGGCTTTCCTTTCT+CGG - chr3.2:69538388-69538407 None:intergenic 50.0%
GTCACAGGCTTTCCTTTCTC+GGG - chr3.2:69538387-69538406 None:intergenic 50.0%
TACGGGATCATTCCCGAGAA+AGG + chr3.2:69538372-69538391 MS.gene25897:CDS 50.0%
TGAGGTTTCTTGTAAGGCGG+AGG - chr3.2:69538726-69538745 None:intergenic 50.0%
TGGAGGTTTGCAAGGCTCAA+GGG + chr3.2:69538343-69538362 MS.gene25897:CDS 50.0%
TGGTACTTGGATATGGCAGC+AGG - chr3.2:69538471-69538490 None:intergenic 50.0%
TTCGGTTGAACGAATGGCTG+AGG - chr3.2:69539383-69539402 None:intergenic 50.0%
TTGATGTTGCCGAGCTTGAG+AGG + chr3.2:69538160-69538179 MS.gene25897:CDS 50.0%
TTGCCTCCATCATTCTTCCC+CGG - chr3.2:69538510-69538529 None:intergenic 50.0%
TTTCCTCCTTGCCTGTTCCT+GGG - chr3.2:69538274-69538293 None:intergenic 50.0%
TTTGTCGCAGCTGGACCTTT+CGG + chr3.2:69538951-69538970 MS.gene25897:CDS 50.0%
! CTGAAGAAGGCATGGAAGGT+TGG + chr3.2:69538750-69538769 MS.gene25897:CDS 50.0%
! GATGGCTCTATCTGGTGATC+TGG + chr3.2:69538038-69538057 MS.gene25897:CDS 50.0%
! GATTTTCCGCTGCGGTTAAG+TGG - chr3.2:69539553-69539572 None:intergenic 50.0%
! GTTTTCCAGACAGGGCTTCT+AGG + chr3.2:69539261-69539280 MS.gene25897:CDS 50.0%
! TAATCTCCGCGAATGGTGGA+AGG + chr3.2:69538419-69538438 MS.gene25897:CDS 50.0%
! TGCTGAATGCTGCTAGCTTG+CGG - chr3.2:69539584-69539603 None:intergenic 50.0%
! TTTTCCTCCTTGCCTGTTCC+TGG - chr3.2:69538275-69538294 None:intergenic 50.0%
!! GGAGCCGAATTTTGATGTGG+AGG + chr3.2:69539016-69539035 MS.gene25897:CDS 50.0%
!! GGTCAAGGAATGGTGATGGA+AGG + chr3.2:69539626-69539645 MS.gene25897:CDS 50.0%
!!! CTCACATCGGTTTTCCAGAC+AGG + chr3.2:69539252-69539271 MS.gene25897:CDS 50.0%
AACACTGTGATCCCCCTCAG+AGG + chr3.2:69538607-69538626 MS.gene25897:CDS 55.0%
AATCCCAGGAACAGGCAAGG+AGG + chr3.2:69538268-69538287 MS.gene25897:CDS 55.0%
ACCGAAACCAATGGCTCAGC+CGG + chr3.2:69539397-69539416 MS.gene25897:CDS 55.0%
ACCGAAGGAAGGATCCGATG+CGG + chr3.2:69538233-69538252 MS.gene25897:CDS 55.0%
AGAGCTGCAGGACACAACCT+TGG + chr3.2:69538560-69538579 MS.gene25897:CDS 55.0%
AGCTTGAGAGGAGGATGTGG+AGG + chr3.2:69538172-69538191 MS.gene25897:CDS 55.0%
AGGATAAAGCTCCCGCGCTA+AGG - chr3.2:69538916-69538935 None:intergenic 55.0%
AGGTCCAGCTGCGACAAATG+GGG - chr3.2:69538949-69538968 None:intergenic 55.0%
ATATGGCAGCAGGACCGTTG+CGG - chr3.2:69538461-69538480 None:intergenic 55.0%
ATCAAGAGGAGGCCTTAGCG+CGG + chr3.2:69538901-69538920 MS.gene25897:CDS 55.0%
ATCCCCCTCAACTTGGGGTT+GGG - chr3.2:69538079-69538098 None:intergenic 55.0%
ATGTTGCCGAGCTTGAGAGG+AGG + chr3.2:69538163-69538182 MS.gene25897:CDS 55.0%
CAATGGAAACCGCCTCTGAG+GGG - chr3.2:69538622-69538641 None:intergenic 55.0%
CAGCTCTTGCAAGGTGTGAG+GGG - chr3.2:69538547-69538566 None:intergenic 55.0%
CAGGGGTCAAGGAATGGTGA+TGG + chr3.2:69539622-69539641 MS.gene25897:CDS 55.0%
CCAATGGAAACCGCCTCTGA+GGG - chr3.2:69538623-69538642 None:intergenic 55.0%
CCACATCCTCCTCTCAAGCT+CGG - chr3.2:69538172-69538191 None:intergenic 55.0%
CGGATAATCTCCGCGAATGG+TGG + chr3.2:69538415-69538434 MS.gene25897:CDS 55.0%
GAAAGGAAAGCCTGTGACCG+GGG + chr3.2:69538389-69538408 MS.gene25897:CDS 55.0%
GAGCTGCAGGACACAACCTT+GGG + chr3.2:69538561-69538580 MS.gene25897:CDS 55.0%
GAGGAGGATGTGGAGGGATA+AGG + chr3.2:69538179-69538198 MS.gene25897:CDS 55.0%
GAGGCGGTTTCCATTGGAGA+AGG + chr3.2:69538626-69538645 MS.gene25897:CDS 55.0%
GCAGCTCTTGCAAGGTGTGA+GGG - chr3.2:69538548-69538567 None:intergenic 55.0%
GCTAACTGAACCTGAGGCGA+TGG + chr3.2:69538110-69538129 MS.gene25897:CDS 55.0%
GCTTGAGAGGAGGATGTGGA+GGG + chr3.2:69538173-69538192 MS.gene25897:CDS 55.0%
GGAAGAGGCTGTGACCAACT+TGG + chr3.2:69539136-69539155 MS.gene25897:CDS 55.0%
GGAGGAACACCCTTCTCCAA+TGG - chr3.2:69538639-69538658 None:intergenic 55.0%
GGCGGAGGACCTTGATCTTT+CGG - chr3.2:69538711-69538730 None:intergenic 55.0%
GTATCCCTAGAAGCCCTGTC+TGG - chr3.2:69539269-69539288 None:intergenic 55.0%
GTGAGGAAGCGGAAGATCTC+TGG + chr3.2:69539164-69539183 MS.gene25897:CDS 55.0%
GTGTGAGGGGTTGGACCAAT+CGG - chr3.2:69538534-69538553 None:intergenic 55.0%
TATCCCCCTCAACTTGGGGT+TGG - chr3.2:69538080-69538099 None:intergenic 55.0%
TCACACCTTGCAAGAGCTGC+AGG + chr3.2:69538548-69538567 MS.gene25897:CDS 55.0%
TCCAATGGAAACCGCCTCTG+AGG - chr3.2:69538624-69538643 None:intergenic 55.0%
TCGCCTCAGGTTCAGTTAGC+CGG - chr3.2:69538110-69538129 None:intergenic 55.0%
TCTTCGTCAACCATCGCCTC+AGG - chr3.2:69538123-69538142 None:intergenic 55.0%
TCTTGCAAGGTGTGAGGGGT+TGG - chr3.2:69538543-69538562 None:intergenic 55.0%
TGATGTCGATGGGGGAGAAG+AGG + chr3.2:69538995-69539014 MS.gene25897:CDS 55.0%
TGCAGCTCTTGCAAGGTGTG+AGG - chr3.2:69538549-69538568 None:intergenic 55.0%
TGTGTCCTGCAGCTCTTGCA+AGG - chr3.2:69538556-69538575 None:intergenic 55.0%
TTCCTGGGATTGCCTCTGCT+TGG - chr3.2:69538259-69538278 None:intergenic 55.0%
TTGAGGGGGATATTACCGCC+CGG + chr3.2:69538088-69538107 MS.gene25897:CDS 55.0%
! AATGGAAACCGCCTCTGAGG+GGG - chr3.2:69538621-69538640 None:intergenic 55.0%
! CCGCGAATGGTGGAAGGATA+AGG + chr3.2:69538425-69538444 MS.gene25897:CDS 55.0%
! CGGCTAAGGAAAGTGCAACC+TGG + chr3.2:69538865-69538884 MS.gene25897:CDS 55.0%
!! ACCGGCTGAGCCATTGGTTT+CGG - chr3.2:69539401-69539420 None:intergenic 55.0%
!! AGAAGGGTGTTCCTCCTCCA+TGG + chr3.2:69538643-69538662 MS.gene25897:CDS 55.0%
AAAGCCTGTGACCGGGGCAT+CGG + chr3.2:69538395-69538414 MS.gene25897:CDS 60.0%
ACTGTGATCCCCCTCAGAGG+CGG + chr3.2:69538610-69538629 MS.gene25897:CDS 60.0%
ATCCCCCCATTTGTCGCAGC+TGG + chr3.2:69538942-69538961 MS.gene25897:CDS 60.0%
ATGGTGGCCTACAGGGAAGG+AGG + chr3.2:69538662-69538681 MS.gene25897:CDS 60.0%
CACCACTCCTCCTTCCCTGT+AGG - chr3.2:69538672-69538691 None:intergenic 60.0%
CCCTCAGAGGCGGTTTCCAT+TGG + chr3.2:69538620-69538639 MS.gene25897:CDS 60.0%
CCGAGCTTGAGAGGAGGATG+TGG + chr3.2:69538169-69538188 MS.gene25897:CDS 60.0%
CGCCTCAGGTTCAGTTAGCC+GGG - chr3.2:69538109-69538128 None:intergenic 60.0%
CTACAGGGAAGGAGGAGTGG+TGG + chr3.2:69538670-69538689 MS.gene25897:CDS 60.0%
CTCAGGTTCAGTTAGCCGGG+CGG - chr3.2:69538106-69538125 None:intergenic 60.0%
CTCCTCCATGGTGGCCTACA+GGG + chr3.2:69538655-69538674 MS.gene25897:CDS 60.0%
GAGGAGTGGTGGCCCCAAAT+TGG + chr3.2:69538681-69538700 MS.gene25897:CDS 60.0%
GCAGAGGCAATCCCAGGAAC+AGG + chr3.2:69538260-69538279 MS.gene25897:CDS 60.0%
GCCGCATCGGATCCTTCCTT+CGG - chr3.2:69538237-69538256 None:intergenic 60.0%
GCGGAGATTATCCGATGCCC+CGG - chr3.2:69538409-69538428 None:intergenic 60.0%
GGCAATCCCAGGAACAGGCA+AGG + chr3.2:69538265-69538284 MS.gene25897:CDS 60.0%
GGTCCAGCTGCGACAAATGG+GGG - chr3.2:69538948-69538967 None:intergenic 60.0%
GTCCAGCTGCGACAAATGGG+GGG - chr3.2:69538947-69538966 None:intergenic 60.0%
TCAAGAGGAGGCCTTAGCGC+GGG + chr3.2:69538902-69538921 MS.gene25897:CDS 60.0%
TCCATGGTGGCCTACAGGGA+AGG + chr3.2:69538659-69538678 MS.gene25897:CDS 60.0%
TCCCCCTCAACTTGGGGTTG+GGG - chr3.2:69538078-69538097 None:intergenic 60.0%
TCCTTCCCTGTAGGCCACCA+TGG - chr3.2:69538663-69538682 None:intergenic 60.0%
TGCTCCCCAACCCCAAGTTG+AGG + chr3.2:69538071-69538090 MS.gene25897:CDS 60.0%
TGGCAGCCACTTAACCGCAG+CGG + chr3.2:69539544-69539563 MS.gene25897:CDS 60.0%
TTATCCGATGCCCCGGTCAC+AGG - chr3.2:69538402-69538421 None:intergenic 60.0%
TTCCCTGTAGGCCACCATGG+AGG - chr3.2:69538660-69538679 None:intergenic 60.0%
TTGCCTCTGCTTGGCCGCAT+CGG - chr3.2:69538250-69538269 None:intergenic 60.0%
! AGGGTGTTCCTCCTCCATGG+TGG + chr3.2:69538646-69538665 MS.gene25897:CDS 60.0%
! CTCCCCAACCCCAAGTTGAG+GGG + chr3.2:69538073-69538092 MS.gene25897:CDS 60.0%
! GCTCCCCAACCCCAAGTTGA+GGG + chr3.2:69538072-69538091 MS.gene25897:CDS 60.0%
! TCCCCAACCCCAAGTTGAGG+GGG + chr3.2:69538074-69538093 MS.gene25897:CDS 60.0%
CCTCCTCCATGGTGGCCTAC+AGG + chr3.2:69538654-69538673 MS.gene25897:CDS 65.0%
CCTGTAGGCCACCATGGAGG+AGG - chr3.2:69538657-69538676 None:intergenic 65.0%
CGCCCGGCTAACTGAACCTG+AGG + chr3.2:69538104-69538123 MS.gene25897:CDS 65.0%
GATCCGATGCGGCCAAGCAG+AGG + chr3.2:69538244-69538263 MS.gene25897:CDS 65.0%
GGCCAAGCAGAGGCAATCCC+AGG + chr3.2:69538254-69538273 MS.gene25897:CDS 65.0%
GGCCTACAGGGAAGGAGGAG+TGG + chr3.2:69538667-69538686 MS.gene25897:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69538015 69539931 69538015 ID=MS.gene25897
chr3.2 mRNA 69538015 69539931 69538015 ID=MS.gene25897.t1;Parent=MS.gene25897
chr3.2 exon 69538015 69539931 69538015 ID=MS.gene25897.t1.exon1;Parent=MS.gene25897.t1
chr3.2 CDS 69538015 69539931 69538015 ID=cds.MS.gene25897.t1;Parent=MS.gene25897.t1
Gene Sequence

>MS.gene25897

ATGATGATGATGTTTGATCATGAGATGGCTCTATCTGGTGATCTGGAAGTGTTTTCTGCTCCCCAACCCCAAGTTGAGGGGGATATTACCGCCCGGCTAACTGAACCTGAGGCGATGGTTGACGAAGATTACAGCGACGATGATATTGATGTTGCCGAGCTTGAGAGGAGGATGTGGAGGGATAAGGTGCTTCTCAAGCGATTAAAGGAGCAAGTCAAACCGAAGGAAGGATCCGATGCGGCCAAGCAGAGGCAATCCCAGGAACAGGCAAGGAGGAAAAAGATGTCAAGGGCTCAAGATGGAATCTTGAAGTACATGCTGAAAATGATGGAGGTTTGCAAGGCTCAAGGGTTTGTTTACGGGATCATTCCCGAGAAAGGAAAGCCTGTGACCGGGGCATCGGATAATCTCCGCGAATGGTGGAAGGATAAGGTTAGGTTTGACCGCAACGGTCCTGCTGCCATATCCAAGTACCAAGCAGACAATGCAATTCCGGGGAAGAATGATGGAGGCAATCCGATTGGTCCAACCCCTCACACCTTGCAAGAGCTGCAGGACACAACCTTGGGTTCTCTCTTATCTGCACTCATGCAACACTGTGATCCCCCTCAGAGGCGGTTTCCATTGGAGAAGGGTGTTCCTCCTCCATGGTGGCCTACAGGGAAGGAGGAGTGGTGGCCCCAAATTGGTTTACCGAAAGATCAAGGTCCTCCGCCTTACAAGAAACCTCATGATCTGAAGAAGGCATGGAAGGTTGGAGTTCTGACTGCTGTCATCAAGCACATGTCTCCCGACATTGCCAAAATTCGCAAGCTTGTAAGGCAATCCAAATGCCTTCAAGATAAAATGACGGCTAAGGAAAGTGCAACCTGGCTTGCAATCGTTAATCAAGAGGAGGCCTTAGCGCGGGAGCTTTATCCTGATTATATCCCCCCATTTGTCGCAGCTGGACCTTTCGGCATCAATGAAGGCAATGAGTATGATGTCGATGGGGGAGAAGAGGAGCCGAATTTTGATGTGGAGGAACGGAAACCTGAGAATCTTCTTCATCAATCCAACATTGGGATGATGGAGAGAATGAGAAATGTAAGACTGCCATTTCAGCAGACTTTCGCAATGAAGGAAGAGGCTGTGACCAACTTGGATTTCGTGAGGAAGCGGAAGATCTCTGGTGAATTTAACATGATGGATCCTAAAATTTTCACTTGCCAACACTCAACATGTCCTTATAGCCAAGCTCACATCGGTTTTCCAGACAGGGCTTCTAGGGATACTCATCAATTGAGTTGTCCATATAGAGGGTCTTCTTCATCTGATTTTGGTGGTCCAAGTTTTCATGCTAATGAGATAAAACCAGTCATATATCCTCAGCCATTCGTTCAACCGAAACCAATGGCTCAGCCGGTTAATATGGTTCCACCTTCCATCGATATAACTGGACTTGGAGTTTCTGAAGATGGTGAGAAAAGGATTGGTGGCCTCATGACTGTCTATGATTCAGACATTCAAGGCAACATGAATTTAACTTCTGGCAGCCACTTAACCGCAGCGGAAAATCACATCTTACCGCAAGCTAGCAGCATTCAGCAGCAGCATGAGAATTACTTCAGGGGTCAAGGAATGGTGATGGAAGGAAACATGTTTGAAGAAACCAACATGTCCAATAACAATCATCATCACATGTTTGCAAGAGATGAAGGTCAATTTGACCAAAGGTTCAAAGCTTTGAATTCTCCCTTTGAGAACAATCACAATCACAATCACAATCAGAATCACAACAATAATTTCCATTTAATGTTTGGGTCCCCACATTGTGATTTGACATCCTATGAGTTCAAGGGAGATATGCATGGAGTAGGCATCATGGATCATCTTCAGAAACAGCCAGATATTTCTTCATCAGTATGGTACCAGTGA
Protein sequence

>MS.gene25897.t1

MMMMFDHEMALSGDLEVFSAPQPQVEGDITARLTEPEAMVDEDYSDDDIDVAELERRMWRDKVLLKRLKEQVKPKEGSDAAKQRQSQEQARRKKMSRAQDGILKYMLKMMEVCKAQGFVYGIIPEKGKPVTGASDNLREWWKDKVRFDRNGPAAISKYQADNAIPGKNDGGNPIGPTPHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVPPPWWPTGKEEWWPQIGLPKDQGPPPYKKPHDLKKAWKVGVLTAVIKHMSPDIAKIRKLVRQSKCLQDKMTAKESATWLAIVNQEEALARELYPDYIPPFVAAGPFGINEGNEYDVDGGEEEPNFDVEERKPENLLHQSNIGMMERMRNVRLPFQQTFAMKEEAVTNLDFVRKRKISGEFNMMDPKIFTCQHSTCPYSQAHIGFPDRASRDTHQLSCPYRGSSSSDFGGPSFHANEIKPVIYPQPFVQPKPMAQPVNMVPPSIDITGLGVSEDGEKRIGGLMTVYDSDIQGNMNLTSGSHLTAAENHILPQASSIQQQHENYFRGQGMVMEGNMFEETNMSNNNHHHMFARDEGQFDQRFKALNSPFENNHNHNHNQNHNNNFHLMFGSPHCDLTSYEFKGDMHGVGIMDHLQKQPDISSSVWYQ