Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25954.t1 | PNY16524.1 | 71.7 | 272 | 77 | 0 | 24 | 295 | 30 | 301 | 1.60E-108 | 403.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25954.t1 | A0A2K3PMN3 | 71.7 | 272 | 77 | 0 | 24 | 295 | 30 | 301 | 1.1e-108 | 403.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25954.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25954.t1 | MTR_2g046410 | 94.309 | 123 | 7 | 0 | 1 | 123 | 1 | 123 | 4.35e-74 | 241 |
| MS.gene25954.t1 | MTR_7g117590 | 67.949 | 156 | 28 | 4 | 479 | 613 | 18 | 172 | 5.39e-59 | 195 |
| MS.gene25954.t1 | MTR_1g054470 | 48.128 | 187 | 37 | 6 | 422 | 602 | 1 | 133 | 7.46e-43 | 150 |
| MS.gene25954.t1 | MTR_2g012700 | 37.383 | 107 | 66 | 1 | 507 | 612 | 13 | 119 | 2.22e-24 | 99.0 |
| MS.gene25954.t1 | MTR_2g062330 | 38.793 | 116 | 66 | 3 | 498 | 612 | 27 | 138 | 1.01e-23 | 97.8 |
| MS.gene25954.t1 | MTR_1914s0010 | 30.131 | 229 | 114 | 4 | 57 | 283 | 28 | 212 | 4.44e-21 | 92.8 |
| MS.gene25954.t1 | MTR_0100s0070 | 36.449 | 107 | 68 | 0 | 63 | 169 | 35 | 141 | 1.09e-18 | 83.6 |
| MS.gene25954.t1 | MTR_1g053380 | 28.372 | 215 | 111 | 2 | 57 | 271 | 85 | 256 | 1.39e-18 | 87.8 |
| MS.gene25954.t1 | MTR_0261s0010 | 36.559 | 93 | 59 | 0 | 57 | 149 | 31 | 123 | 1.19e-17 | 79.7 |
| MS.gene25954.t1 | MTR_8g019530 | 41.667 | 84 | 49 | 0 | 66 | 149 | 196 | 279 | 3.44e-17 | 82.8 |
| MS.gene25954.t1 | MTR_8g461860 | 34.000 | 100 | 63 | 1 | 51 | 147 | 21 | 120 | 1.17e-15 | 74.3 |
| MS.gene25954.t1 | MTR_5g071400 | 39.423 | 104 | 12 | 4 | 422 | 519 | 1 | 59 | 1.61e-12 | 64.3 |
| MS.gene25954.t1 | MTR_0010s0310 | 33.333 | 90 | 57 | 1 | 517 | 603 | 151 | 240 | 4.42e-12 | 67.0 |
| MS.gene25954.t1 | MTR_0058s0020 | 40.000 | 70 | 42 | 0 | 51 | 120 | 22 | 91 | 6.65e-12 | 62.4 |
| MS.gene25954.t1 | MTR_0718s0040 | 38.889 | 72 | 44 | 0 | 57 | 128 | 31 | 102 | 8.17e-12 | 62.4 |
| MS.gene25954.t1 | MTR_8g445590 | 40.625 | 64 | 38 | 0 | 57 | 120 | 29 | 92 | 1.48e-11 | 61.6 |
| MS.gene25954.t1 | MTR_8g031840 | 46.552 | 58 | 31 | 0 | 90 | 147 | 220 | 277 | 2.43e-11 | 65.1 |
| MS.gene25954.t1 | MTR_1g053430 | 37.500 | 72 | 45 | 0 | 57 | 128 | 31 | 102 | 3.56e-11 | 60.8 |
| MS.gene25954.t1 | MTR_1g053410 | 37.500 | 72 | 45 | 0 | 57 | 128 | 31 | 102 | 3.56e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 131 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCTAATAGATAGTTATTT+TGG | 0.087942 | 3.2:+68945579 | MS.gene25954:intron |
| TTGTTAGTTCTAGCACTTCT+AGG | 0.215193 | 3.2:-68940269 | None:intergenic |
| TTTGTGGTGCCTTTCCCTTC+TGG | 0.237506 | 3.2:-68940595 | None:intergenic |
| CACGATCCTCCACAATTTCT+TGG | 0.249202 | 3.2:+68940029 | MS.gene25954:CDS |
| CGTCCAGTCACATATGCTTT+TGG | 0.265470 | 3.2:+68940668 | MS.gene25954:CDS |
| GTGGATGGTTTGAGTTTGTT+AGG | 0.273672 | 3.2:+68947585 | MS.gene25954:CDS |
| ATATGCATCTTACTTACTAT+TGG | 0.285200 | 3.2:+68940142 | MS.gene25954:CDS |
| ACAACCAATCTTCGTCAATT+TGG | 0.295043 | 3.2:-68940391 | None:intergenic |
| TGTTATCAATGTGGAGATTC+TGG | 0.299201 | 3.2:+68940794 | MS.gene25954:CDS |
| GGCGAACACTTGTCCACTTA+AGG | 0.313502 | 3.2:+68940763 | MS.gene25954:CDS |
| AAACACTTTCGGATATTGTT+TGG | 0.325553 | 3.2:-68940716 | None:intergenic |
| TGATTATTTCTACGAGTCAT+TGG | 0.345404 | 3.2:-68939822 | None:intergenic |
| ATGAGTATTCTAGGTGATAT+TGG | 0.358611 | 3.2:+68945603 | MS.gene25954:CDS |
| AGGACCAAATTGACGAAGAT+TGG | 0.367289 | 3.2:+68940387 | MS.gene25954:CDS |
| TGAAGATCGGGTCATTTGAT+TGG | 0.369943 | 3.2:+68946903 | MS.gene25954:CDS |
| AATCATATAGAAGCGGATAA+TGG | 0.370038 | 3.2:+68939840 | MS.gene25954:CDS |
| AATTCGTCAGTTTCAACCTT+TGG | 0.371123 | 3.2:+68940472 | MS.gene25954:CDS |
| TATGCATCTTACTTACTATT+GGG | 0.372620 | 3.2:+68940143 | MS.gene25954:CDS |
| CGAATTGTTGATGATGAAAC+TGG | 0.373851 | 3.2:+68947503 | MS.gene25954:CDS |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 3.2:+68947536 | MS.gene25954:CDS |
| TCCTCTAACAGTTGGTTTGA+CGG | 0.383333 | 3.2:-68940876 | None:intergenic |
| AGTTCCCTTCCACCAGTATT+CGG | 0.383466 | 3.2:-68940171 | None:intergenic |
| GCGAACACTTGTCCACTTAA+GGG | 0.385058 | 3.2:+68940764 | MS.gene25954:CDS |
| GGCTGCTGGACGGAGAGTTC+TGG | 0.386406 | 3.2:+68947318 | MS.gene25954:CDS |
| TGGCCATCGACTTCTTCATT+TGG | 0.387700 | 3.2:-68947223 | None:intergenic |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 3.2:+68947543 | MS.gene25954:CDS |
| GCTCTTACATTGTTCACCTT+TGG | 0.390118 | 3.2:-68940854 | None:intergenic |
| TCTGACATTTGAAACACTTT+CGG | 0.397857 | 3.2:-68940727 | None:intergenic |
| TCAGCGAAGAACGATGAAAA+TGG | 0.399065 | 3.2:+68946811 | MS.gene25954:CDS |
| TTTGAAATTGTGCAAATACT+TGG | 0.402550 | 3.2:-68947243 | None:intergenic |
| CTAACAATCAAGATATTACT+TGG | 0.404224 | 3.2:+68940210 | MS.gene25954:CDS |
| TATGTTACCTTTACTTCGTC+AGG | 0.404680 | 3.2:-68940122 | None:intergenic |
| GAACGAAACGCTGAAAATAA+AGG | 0.405688 | 3.2:+68939987 | MS.gene25954:CDS |
| CTGGAAGAAACGAAAATGTT+TGG | 0.405716 | 3.2:-68940366 | None:intergenic |
| ATTAGTTCTGCTACGGCTGC+TGG | 0.408831 | 3.2:+68947304 | MS.gene25954:CDS |
| TATTGGGAGATGCCGAATAC+TGG | 0.420095 | 3.2:+68940159 | MS.gene25954:CDS |
| TGTTGATCGCGGTTGTTACC+TGG | 0.422067 | 3.2:-68940638 | None:intergenic |
| CGCAGCAGTTTGTTCTGCAA+TGG | 0.424302 | 3.2:-68939928 | None:intergenic |
| GAAGATCGGGTCATTTGATT+GGG | 0.425740 | 3.2:+68946904 | MS.gene25954:CDS |
| AGCTCTTTCCATACCTCCTT+CGG | 0.426303 | 3.2:-68939877 | None:intergenic |
| TATTCAAGCTATGGCAGAAA+TGG | 0.428109 | 3.2:+68939899 | MS.gene25954:CDS |
| AGCCATAAGTCAGCTTTCTC+TGG | 0.430631 | 3.2:-68940062 | None:intergenic |
| ATGTCAGAAAACCGACCATT+TGG | 0.443654 | 3.2:+68940742 | MS.gene25954:CDS |
| CGAAGAAGAAGTTGAAGATC+GGG | 0.445939 | 3.2:+68946891 | MS.gene25954:CDS |
| ACAATGTAGGAGGACCATCT+AGG | 0.447973 | 3.2:+68940546 | MS.gene25954:CDS |
| ATGAAATGTGCGGCGGATGC+AGG | 0.449012 | 3.2:+68946840 | MS.gene25954:CDS |
| AACCAGAGAAAGCTGACTTA+TGG | 0.450437 | 3.2:+68940060 | MS.gene25954:CDS |
| GCCGTCAAACCAACTGTTAG+AGG | 0.456683 | 3.2:+68940875 | MS.gene25954:CDS |
| TTCGGATATTGTTTGGAGGT+TGG | 0.460778 | 3.2:-68940709 | None:intergenic |
| TTCTGGTTTGATCCTTGTGA+AGG | 0.473075 | 3.2:-68940578 | None:intergenic |
| AACTGCTGCGAAGGCTGAAA+GGG | 0.475894 | 3.2:+68939941 | MS.gene25954:CDS |
| AAGATAGAAAGGATGAAAGA+AGG | 0.480804 | 3.2:+68940509 | MS.gene25954:CDS |
| TGGATGGTTTGAGTTTGTTA+GGG | 0.484898 | 3.2:+68947586 | MS.gene25954:CDS |
| TCAATCAGAAGATCTCATAC+AGG | 0.485592 | 3.2:+68940931 | MS.gene25954:CDS |
| GCATATGTGACTGGACGATA+AGG | 0.486202 | 3.2:-68940662 | None:intergenic |
| TAACATATCAATCCCTTAAG+TGG | 0.487127 | 3.2:-68940776 | None:intergenic |
| GTGGACAAGTGTTCGCCAAA+TGG | 0.493798 | 3.2:-68940757 | None:intergenic |
| CAATGTAGGAGGACCATCTA+GGG | 0.496149 | 3.2:+68940547 | MS.gene25954:CDS |
| TTCCACCTTGTTTCAAGAGA+AGG | 0.496454 | 3.2:-68940304 | None:intergenic |
| TGGTTGTGTGAAAGATTTGA+AGG | 0.505098 | 3.2:+68940407 | MS.gene25954:CDS |
| CTGCAGCGATGTAATTGATC+CGG | 0.509939 | 3.2:+68947168 | MS.gene25954:intron |
| AATCTTCGTCAATTTGGTCC+TGG | 0.514034 | 3.2:-68940385 | None:intergenic |
| CAGGGAAATTGCGATATGTC+AGG | 0.515047 | 3.2:+68940950 | MS.gene25954:CDS |
| TGGACTTAGATTTAGTATCA+AGG | 0.515780 | 3.2:+68940430 | MS.gene25954:CDS |
| CACTTTCGGATATTGTTTGG+AGG | 0.521432 | 3.2:-68940713 | None:intergenic |
| GTTACCTGGAGGTCGTGAGT+AGG | 0.522751 | 3.2:-68940624 | None:intergenic |
| CAAGGAGTCTGTATTGCCAT+TGG | 0.523897 | 3.2:+68940448 | MS.gene25954:CDS |
| AAACCCTACTCACGACCTCC+AGG | 0.524550 | 3.2:+68940620 | MS.gene25954:CDS |
| TTACCTGGAGGTCGTGAGTA+GGG | 0.524713 | 3.2:-68940623 | None:intergenic |
| AAACTGCTGCGAAGGCTGAA+AGG | 0.529868 | 3.2:+68939940 | MS.gene25954:CDS |
| GGAAGAGTTTACACTCTGAA+TGG | 0.532813 | 3.2:+68940896 | MS.gene25954:CDS |
| CTTCTTCGTCCATCTCAAAA+CGG | 0.535091 | 3.2:-68946876 | None:intergenic |
| GATATTGGAGCTGCTTTAGA+CGG | 0.535842 | 3.2:+68945618 | MS.gene25954:CDS |
| AAGCAATACCGAAGGAGGTA+TGG | 0.538428 | 3.2:+68939869 | MS.gene25954:CDS |
| ACGAAGAAGAAGTTGAAGAT+CGG | 0.540825 | 3.2:+68946890 | MS.gene25954:CDS |
| TGCAGAACAAACTGCTGCGA+AGG | 0.541411 | 3.2:+68939932 | MS.gene25954:CDS |
| CGATGTAATTGATCCGGAGT+TGG | 0.544205 | 3.2:+68947174 | MS.gene25954:CDS |
| CAATCTTATTGCCGTCAAAA+CGG | 0.544280 | 3.2:-68946936 | None:intergenic |
| TTATTGCCGTCAAAACGGTT+CGG | 0.545486 | 3.2:-68946931 | None:intergenic |
| GGAAAGAGCTATTCAAGCTA+TGG | 0.545696 | 3.2:+68939890 | MS.gene25954:CDS |
| GGATATTCGAAAGCAACAAA+GGG | 0.546128 | 3.2:+68939962 | MS.gene25954:CDS |
| GGAATTAATCGAAACAATGT+AGG | 0.547247 | 3.2:+68940533 | MS.gene25954:CDS |
| GGGATATTCGAAAGCAACAA+AGG | 0.547460 | 3.2:+68939961 | MS.gene25954:CDS |
| TAAACTCTTCCTCTAACAGT+TGG | 0.550196 | 3.2:-68940884 | None:intergenic |
| CCTCCAAAAGCATATGTGAC+TGG | 0.553269 | 3.2:-68940671 | None:intergenic |
| CTTTGAATTGCAATCTTCCG+AGG | 0.557056 | 3.2:-68947455 | None:intergenic |
| GAGGTGTCCTGACGAAGTAA+AGG | 0.560366 | 3.2:+68940115 | MS.gene25954:CDS |
| ATAGAAAGGATGAAAGAAGG+TGG | 0.560415 | 3.2:+68940512 | MS.gene25954:CDS |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 3.2:+68947547 | MS.gene25954:CDS |
| GGTTTCAATTCGTCCAACTC+CGG | 0.567151 | 3.2:-68947187 | None:intergenic |
| TCAGTTTCTCCAAGAAATTG+TGG | 0.569494 | 3.2:-68940038 | None:intergenic |
| TAGAAATAATCATATAGAAG+CGG | 0.571524 | 3.2:+68939833 | MS.gene25954:CDS |
| AGAAATACGTTGGAAGTGGA+TGG | 0.575514 | 3.2:+68947570 | MS.gene25954:CDS |
| ATGGAGAAATACGTTGGAAG+TGG | 0.576439 | 3.2:+68947566 | MS.gene25954:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.580120 | 3.2:+68947297 | MS.gene25954:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.583531 | 3.2:+68947560 | MS.gene25954:CDS |
| AGATGCCGAATACTGGTGGA+AGG | 0.583781 | 3.2:+68940166 | MS.gene25954:CDS |
| CAATCAGAAGATCTCATACA+GGG | 0.584467 | 3.2:+68940932 | MS.gene25954:CDS |
| CCATCTCAAAACGGCGACGA+TGG | 0.584489 | 3.2:-68946867 | None:intergenic |
| AGGGCAAAAGAACTCAAAGA+AGG | 0.584629 | 3.2:+68947605 | MS.gene25954:CDS |
| TTAATGTGTAGCGTATTCCT+CGG | 0.586314 | 3.2:+68947438 | MS.gene25954:intron |
| GCATCCGCCGCACATTTCAT+CGG | 0.590420 | 3.2:-68946837 | None:intergenic |
| ATTAATCGAAACAATGTAGG+AGG | 0.591932 | 3.2:+68940536 | MS.gene25954:CDS |
| GATACAAATAATTTGTGTGG+AGG | 0.593345 | 3.2:-68947656 | None:intergenic |
| TTCCTTCTCTTGAAACAAGG+TGG | 0.597538 | 3.2:+68940302 | MS.gene25954:CDS |
| AAAAGATATTCGCTGTGTTG+AGG | 0.600068 | 3.2:+68940096 | MS.gene25954:CDS |
| TGAAACTGACGAATTTCCAA+TGG | 0.606151 | 3.2:-68940464 | None:intergenic |
| TGGGAGATGCCGAATACTGG+TGG | 0.606725 | 3.2:+68940162 | MS.gene25954:CDS |
| ACTGATACAAATAATTTGTG+TGG | 0.607437 | 3.2:-68947659 | None:intergenic |
| TAACAATCAAGATATTACTT+GGG | 0.608606 | 3.2:+68940211 | MS.gene25954:CDS |
| TTCACAAGGATCAAACCAGA+AGG | 0.611115 | 3.2:+68940580 | MS.gene25954:CDS |
| ACAAGTGTTCGCCAAATGGT+CGG | 0.618562 | 3.2:-68940753 | None:intergenic |
| GAGTTCCTTCTCTTGAAACA+AGG | 0.621249 | 3.2:+68940299 | MS.gene25954:CDS |
| GTTCTGCTACGGCTGCTGGA+CGG | 0.628877 | 3.2:+68947308 | MS.gene25954:CDS |
| GACGATAAGGTTGTTGATCG+CGG | 0.628881 | 3.2:-68940649 | None:intergenic |
| GAAGCAAGCAATACCGAAGG+AGG | 0.630174 | 3.2:+68939864 | MS.gene25954:CDS |
| GTTTCTCCAAGAAATTGTGG+AGG | 0.634446 | 3.2:-68940035 | None:intergenic |
| AGGATCAAACCAGAAGGGAA+AGG | 0.637728 | 3.2:+68940586 | MS.gene25954:CDS |
| TTGTGTGAAAGATTTGAAGG+TGG | 0.649221 | 3.2:+68940410 | MS.gene25954:CDS |
| GATGCCGAATACTGGTGGAA+GGG | 0.658778 | 3.2:+68940167 | MS.gene25954:CDS |
| GGAGAAGCAAGCAATACCGA+AGG | 0.661940 | 3.2:+68939861 | MS.gene25954:CDS |
| GGATTGATATGTTATCAATG+TGG | 0.663181 | 3.2:+68940785 | MS.gene25954:CDS |
| TGATCGCGGTTGTTACCTGG+AGG | 0.663250 | 3.2:-68940635 | None:intergenic |
| TCACAAGGATCAAACCAGAA+GGG | 0.665621 | 3.2:+68940581 | MS.gene25954:CDS |
| AATCCAAATGAAGAAGTCGA+TGG | 0.671453 | 3.2:+68947220 | MS.gene25954:CDS |
| AATGACAATTGATGAATATG+CGG | 0.685433 | 3.2:+68940325 | MS.gene25954:CDS |
| ACAACTTTCGAAGAAACCGA+AGG | 0.692098 | 3.2:+68945654 | MS.gene25954:CDS |
| AATGTAGGAGGACCATCTAG+GGG | 0.692301 | 3.2:+68940548 | MS.gene25954:CDS |
| ATGTAGGAGGACCATCTAGG+GGG | 0.715480 | 3.2:+68940549 | MS.gene25954:CDS |
| ATGGCGACCGATGAAATGTG+CGG | 0.726557 | 3.2:+68946830 | MS.gene25954:CDS |
| GCGACCGATGAAATGTGCGG+CGG | 0.753822 | 3.2:+68946833 | MS.gene25954:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 68939825 | 68947691 | 68939825 | ID=MS.gene25954 |
| chr3.2 | mRNA | 68939825 | 68947691 | 68939825 | ID=MS.gene25954.t1;Parent=MS.gene25954 |
| chr3.2 | exon | 68939825 | 68940971 | 68939825 | ID=MS.gene25954.t1.exon1;Parent=MS.gene25954.t1 |
| chr3.2 | CDS | 68939825 | 68940971 | 68939825 | ID=cds.MS.gene25954.t1;Parent=MS.gene25954.t1 |
| chr3.2 | exon | 68945589 | 68945675 | 68945589 | ID=MS.gene25954.t1.exon2;Parent=MS.gene25954.t1 |
| chr3.2 | CDS | 68945589 | 68945675 | 68945589 | ID=cds.MS.gene25954.t1;Parent=MS.gene25954.t1 |
| chr3.2 | exon | 68946795 | 68946993 | 68946795 | ID=MS.gene25954.t1.exon3;Parent=MS.gene25954.t1 |
| chr3.2 | CDS | 68946795 | 68946993 | 68946795 | ID=cds.MS.gene25954.t1;Parent=MS.gene25954.t1 |
| chr3.2 | exon | 68947174 | 68947339 | 68947174 | ID=MS.gene25954.t1.exon4;Parent=MS.gene25954.t1 |
| chr3.2 | CDS | 68947174 | 68947339 | 68947174 | ID=cds.MS.gene25954.t1;Parent=MS.gene25954.t1 |
| chr3.2 | exon | 68947449 | 68947691 | 68947449 | ID=MS.gene25954.t1.exon5;Parent=MS.gene25954.t1 |
| chr3.2 | CDS | 68947449 | 68947691 | 68947449 | ID=cds.MS.gene25954.t1;Parent=MS.gene25954.t1 |
| Gene Sequence |
| Protein sequence |
