AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26042

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26042.t1 MTR_6g044690 94.700 566 29 1 1 565 1 566 0.0 1085
MS.gene26042.t1 MTR_6g043960 88.889 504 36 1 62 565 1 484 0.0 919
MS.gene26042.t1 MTR_0376s0030 75.979 562 130 3 7 566 7 565 0.0 873
MS.gene26042.t1 MTR_2g054460 82.398 517 39 4 52 566 1 467 0.0 854
MS.gene26042.t1 MTR_1g035420 57.243 573 200 11 5 563 105 646 0.0 630
MS.gene26042.t1 MTR_8g070070 49.755 613 232 12 12 563 160 757 0.0 575
MS.gene26042.t1 MTR_8g070075 50.444 563 217 11 55 563 196 750 0.0 562
MS.gene26042.t1 MTR_8g071030 51.648 546 219 9 55 563 200 737 0.0 559
MS.gene26042.t1 MTR_1g035400 54.215 522 207 9 55 563 63 565 0.0 542
MS.gene26042.t1 MTR_7g088370 37.678 491 258 10 92 563 219 680 1.34e-98 315
MS.gene26042.t1 MTR_8g042750 35.893 521 288 15 60 563 167 658 2.07e-90 293
MS.gene26042.t1 MTR_5g013420 38.031 518 265 17 60 561 581 1058 2.09e-89 298
MS.gene26042.t1 MTR_5g013420 38.031 518 265 17 60 561 581 1058 2.62e-89 298
MS.gene26042.t1 MTR_4g010830 38.511 470 245 14 109 561 507 949 6.69e-89 295
MS.gene26042.t1 MTR_3g011440 34.151 530 289 13 60 564 141 635 8.76e-89 288
MS.gene26042.t1 MTR_4g010550 36.117 479 235 17 98 561 11 433 1.19e-79 258
MS.gene26042.t1 MTR_6g061270 34.756 492 260 16 109 562 196 664 6.95e-79 262
MS.gene26042.t1 MTR_7g084090 34.870 499 271 16 99 562 248 727 1.23e-77 260
MS.gene26042.t1 MTR_6g061200 31.551 561 302 19 34 562 125 635 5.05e-72 243
MS.gene26042.t1 MTR_6g044000 85.833 120 17 0 62 181 1 120 3.95e-64 207
MS.gene26042.t1 MTR_6g044000 100.000 56 0 0 511 566 117 172 3.46e-33 125
MS.gene26042.t1 MTR_7g084090 37.076 383 192 14 99 451 248 611 3.69e-63 219
MS.gene26042.t1 MTR_1g048950 32.230 453 253 11 130 561 88 507 1.31e-49 180
MS.gene26042.t1 MTR_7g445710 29.638 469 241 14 114 561 112 512 2.96e-48 176
MS.gene26042.t1 MTR_7g445530 29.424 469 242 14 114 561 112 512 7.70e-48 174
MS.gene26042.t1 MTR_7g450730 29.888 445 230 14 117 561 112 474 3.75e-44 164
MS.gene26042.t1 MTR_7g052480 29.524 315 194 13 263 562 225 526 2.46e-31 128
MS.gene26042.t1 MTR_7g445600 31.788 151 65 2 411 561 243 355 2.86e-20 93.2
MS.gene26042.t1 MTR_7g445600 33.058 121 75 3 114 233 108 223 1.45e-11 66.6
MS.gene26042.t1 MTR_8g078505 29.609 179 112 2 386 564 354 518 2.84e-19 91.7
MS.gene26042.t1 MTR_5g018850 30.000 180 113 3 380 558 1313 1480 1.60e-18 90.5
MS.gene26042.t1 MTR_1g069570 22.801 307 171 8 309 564 430 721 1.70e-17 86.7
MS.gene26042.t1 MTR_4g132610 28.723 188 117 4 380 564 853 1026 3.50e-17 85.9
MS.gene26042.t1 MTR_8g078490 29.255 188 116 4 380 564 1059 1232 3.86e-17 85.9
MS.gene26042.t1 MTR_5g098680 32.484 157 86 4 137 280 267 416 1.67e-14 77.0
MS.gene26042.t1 MTR_1g069570 23.282 262 136 7 309 520 430 676 1.13e-13 74.3
MS.gene26042.t1 MTR_4g131630 61.538 52 17 1 508 559 1 49 2.50e-12 62.0
MS.gene26042.t1 MTR_8g102580 30.573 157 89 4 137 280 261 410 3.34e-12 69.7
MS.gene26042.t1 MTR_7g445810 30.405 148 94 3 117 261 128 269 4.64e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26042.t1 AT4G13460 49.136 521 229 9 56 563 141 638 2.07e-169 496
MS.gene26042.t1 AT4G13460 49.136 521 229 9 56 563 141 638 2.07e-169 496
MS.gene26042.t1 AT2G33290 48.308 532 238 11 45 563 132 639 5.10e-167 489
MS.gene26042.t1 AT2G33290 48.308 532 238 11 45 563 132 639 5.10e-167 489
MS.gene26042.t1 AT2G33290 48.308 532 238 11 45 563 132 639 5.10e-167 489
MS.gene26042.t1 AT2G33290 48.308 532 238 11 45 563 132 639 5.10e-167 489
MS.gene26042.t1 AT2G22740 35.425 494 280 12 88 561 285 759 2.27e-87 287
MS.gene26042.t1 AT2G22740 35.425 494 280 12 88 561 285 759 2.27e-87 287
MS.gene26042.t1 AT2G35160 36.344 465 232 13 114 561 310 727 1.78e-83 276
MS.gene26042.t1 AT2G35160 36.344 465 232 13 114 561 346 763 4.22e-83 276
MS.gene26042.t1 AT2G35160 36.344 465 232 13 114 561 346 763 4.22e-83 276
MS.gene26042.t1 AT2G35160 36.344 465 232 13 114 561 346 763 4.22e-83 276
MS.gene26042.t1 AT2G35160 36.344 465 232 13 114 561 346 763 4.22e-83 276
MS.gene26042.t1 AT1G73100 35.262 553 306 18 35 563 115 639 9.32e-83 272
MS.gene26042.t1 AT5G04940 34.749 518 292 14 60 563 155 640 1.10e-82 272
MS.gene26042.t1 AT5G04940 34.749 518 292 14 60 563 155 640 1.10e-82 272
MS.gene26042.t1 AT5G13960 35.331 484 268 19 109 562 126 594 4.34e-77 256
MS.gene26042.t1 AT5G13960 35.421 463 255 18 130 562 12 460 1.07e-76 251
MS.gene26042.t1 AT1G17770 34.978 446 266 11 132 563 226 661 9.93e-74 248
MS.gene26042.t1 AT2G24740 36.902 439 253 13 129 563 306 724 3.98e-72 245
MS.gene26042.t1 AT5G47150 35.361 263 131 9 26 279 92 324 2.64e-32 127
MS.gene26042.t1 AT2G23740 29.210 291 150 10 305 562 1089 1356 1.14e-24 109
MS.gene26042.t1 AT2G23740 29.210 291 150 10 305 562 1089 1356 1.14e-24 109
MS.gene26042.t1 AT2G23740 29.210 291 150 10 305 562 1089 1356 1.14e-24 109
MS.gene26042.t1 AT2G23740 29.210 291 150 10 305 562 1082 1349 1.22e-24 109
MS.gene26042.t1 AT5G47160 37.569 181 95 6 106 276 236 408 9.69e-24 104
MS.gene26042.t1 AT5G47160 37.569 181 95 6 106 276 236 408 9.69e-24 104
MS.gene26042.t1 AT5G47160 37.569 181 95 6 106 276 236 408 9.69e-24 104
MS.gene26042.t1 AT2G23740 30.120 249 118 10 305 520 1089 1314 1.05e-19 94.0
MS.gene26042.t1 AT2G23740 30.120 249 118 10 305 520 1089 1314 1.05e-19 94.0
MS.gene26042.t1 AT3G03750 27.636 275 159 7 309 562 68 323 1.09e-18 88.2
MS.gene26042.t1 AT2G05900 37.572 173 92 9 174 337 26 191 1.02e-17 84.7
MS.gene26042.t1 AT1G57820 30.233 172 90 6 132 280 263 427 3.70e-13 72.8
MS.gene26042.t1 AT1G57820 30.233 172 90 6 132 280 263 427 3.70e-13 72.8
MS.gene26042.t1 AT1G57820 30.769 169 90 7 132 280 263 424 7.02e-13 72.0
MS.gene26042.t1 AT5G39550 30.233 172 90 7 132 280 248 412 8.35e-12 68.6

Find 129 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTTTGATCATGGTAAT+TGG 0.179510 7.4:+53944651 MS.gene26042:CDS
AGTGAATGTTGGTTTGAAAA+AGG 0.258242 7.4:+53943822 MS.gene26042:CDS
GATTCGTGTGTCGGGTTTGC+TGG 0.262976 7.4:+53943275 MS.gene26042:CDS
ACATAGGATTATCACATAAT+TGG 0.283098 7.4:-53943947 None:intergenic
TTGGATGTTTCCCCATCTTA+TGG 0.286358 7.4:+53944670 MS.gene26042:CDS
AGGATCATACTACCCATCTT+AGG 0.317543 7.4:-53943897 None:intergenic
TTGGCCTCTCTTAAGCATAT+TGG 0.319702 7.4:-53943928 None:intergenic
TGGAACGATGGCGCGCAATA+TGG 0.334616 7.4:-53943085 None:intergenic
AGAAACCGTGTGACACAAAA+AGG 0.338747 7.4:+53944281 MS.gene26042:CDS
CCAGTTCCTGGTGTTTGTAT+TGG 0.341157 7.4:+53943459 MS.gene26042:CDS
ATATTGCGCGCCATCGTTCC+AGG 0.358468 7.4:+53943087 MS.gene26042:CDS
CATGCTAGTATGAGTTCTAA+TGG 0.360661 7.4:+53943558 MS.gene26042:CDS
ATGAAACTAGTTGGGGAGTT+AGG 0.362684 7.4:+53944336 MS.gene26042:CDS
TATTATTCACTTCTTCTGTC+TGG 0.364776 7.4:-53943353 None:intergenic
TCCAATGGGAAGGAGAATAT+TGG 0.376182 7.4:+53943990 MS.gene26042:CDS
CAGAAGAAGTGAATAATAGT+AGG 0.376433 7.4:+53943358 MS.gene26042:CDS
ACTAATTGAACAAACACATT+TGG 0.377806 7.4:-53944629 None:intergenic
AACATTGGAAATGCCGCCTT+CGG 0.378400 7.4:-53944071 None:intergenic
TAGATCTAATGAAACTAGTT+GGG 0.379002 7.4:+53944328 MS.gene26042:CDS
ATAAAAGTTCCAGCTAGAAT+AGG 0.380021 7.4:-53944368 None:intergenic
TAAGCATATTGGCTTCCTTA+AGG 0.394112 7.4:-53943917 None:intergenic
TGCGAAGGACAACTACAAAA+AGG 0.399229 7.4:-53944254 None:intergenic
TAGCAGCAATCAAGTCATTT+CGG 0.407660 7.4:-53943383 None:intergenic
CAATTAGTTCTCTTTGATCA+TGG 0.411850 7.4:+53944644 MS.gene26042:CDS
TTTATAAGTTTATGTTATCA+AGG 0.412998 7.4:+53943862 MS.gene26042:CDS
TTCCTCGTCGTCATACTTTG+TGG 0.413262 7.4:-53944101 None:intergenic
GTTTGATTCGATTCGTGTGT+CGG 0.415574 7.4:+53943266 MS.gene26042:CDS
AAATCTAATCAGAAGATTAG+AGG 0.415891 7.4:+53943162 MS.gene26042:CDS
GAATAGGTCTACTCAAGAAT+GGG 0.418852 7.4:+53944490 MS.gene26042:CDS
TAGTTGGGGAGTTAGGTCTT+TGG 0.420703 7.4:+53944343 MS.gene26042:CDS
CTTGATCATGGGGTAGAAGC+TGG 0.421865 7.4:+53944734 MS.gene26042:CDS
TAAAAGTTCCAGCTAGAATA+GGG 0.427435 7.4:-53944367 None:intergenic
GCGCGCAATATGGAAGGGTT+TGG 0.428940 7.4:-53943075 None:intergenic
GAACAAACACATTTGGAGTT+GGG 0.430179 7.4:-53944622 None:intergenic
ACATTGGAAATGCCGCCTTC+GGG 0.431588 7.4:-53944070 None:intergenic
CCATTGCAAATACCATAAGA+TGG 0.432457 7.4:-53944682 None:intergenic
AAAATCATTGGTCCAGTTCC+TGG 0.434098 7.4:+53943447 MS.gene26042:CDS
GTCCGTATCGTGAGATGGTC+CGG 0.436727 7.4:+53943229 MS.gene26042:CDS
TGAATAGGTCTACTCAAGAA+TGG 0.436832 7.4:+53944489 MS.gene26042:CDS
TGTATAAGATAAGTGAATGT+TGG 0.437441 7.4:+53943811 MS.gene26042:CDS
GGTTCAAGGTCAACCTAAGA+TGG 0.443665 7.4:+53943884 MS.gene26042:CDS
CAAACAAAATTCTTGTTCTC+CGG 0.445353 7.4:-53943248 None:intergenic
TTAGATCTAATGAAACTAGT+TGG 0.450265 7.4:+53944327 MS.gene26042:CDS
TGGTACTTCTACTACTAGTA+AGG 0.451010 7.4:+53943770 MS.gene26042:CDS
GAGTGTTCTGAATGCGTTGA+TGG 0.451024 7.4:+53944140 MS.gene26042:CDS
TGCAATATGAGATTGATGTC+AGG 0.456152 7.4:+53943721 MS.gene26042:CDS
GATGGGTAGTATGATCCTTA+AGG 0.457082 7.4:+53943902 MS.gene26042:CDS
CCATCTTATGGTATTTGCAA+TGG 0.459327 7.4:+53944682 MS.gene26042:CDS
TTTGATTCGATTCGTGTGTC+GGG 0.459511 7.4:+53943267 MS.gene26042:CDS
CCAATACAAACACCAGGAAC+TGG 0.467377 7.4:-53943459 None:intergenic
TGAACAAACACATTTGGAGT+TGG 0.468046 7.4:-53944623 None:intergenic
CTTTCTGATGATATTTCCAA+TGG 0.469201 7.4:+53943975 MS.gene26042:CDS
CTCGTCGGGACCTGGAACGA+TGG 0.470882 7.4:-53943097 None:intergenic
CATTGCAAATACCATAAGAT+GGG 0.474185 7.4:-53944681 None:intergenic
TATCATGATGTGAAAATCAT+TGG 0.477666 7.4:+53943435 MS.gene26042:CDS
AGAAGTGAAATGTGTGTGAT+TGG 0.479419 7.4:+53943498 MS.gene26042:CDS
GCCAATATTCTCCTTCCCAT+TGG 0.487788 7.4:-53943991 None:intergenic
CTCCCTAGTCAAAACAAGTC+CGG 0.496317 7.4:-53944402 None:intergenic
ACTACCCCTTATAGTAAAAG+TGG 0.498302 7.4:+53944191 MS.gene26042:CDS
ACTAGTGTGGTTATGTCTGA+TGG 0.506532 7.4:+53943594 MS.gene26042:CDS
AAAAGGTATTAAATATAGGT+TGG 0.507633 7.4:+53944298 MS.gene26042:CDS
TCAAGACTAAGCTCTCTCAT+TGG 0.507939 7.4:-53944716 None:intergenic
TCTTTGGACCCTATTCTAGC+TGG 0.516120 7.4:+53944359 MS.gene26042:CDS
TTTATGTTATCAAGGGTTCA+AGG 0.516545 7.4:+53943870 MS.gene26042:CDS
AAATCTAAAGTAGGATATGC+GGG 0.520737 7.4:-53944548 None:intergenic
TACGACAGCAACACTCTGGC+GGG 0.520756 7.4:+53943129 MS.gene26042:CDS
AGAGAGCTTAGTCTTGATCA+TGG 0.523319 7.4:+53944722 MS.gene26042:CDS
AGAGTTATGTTCACCATCCA+TGG 0.526426 7.4:-53944441 None:intergenic
TGATGATATTTCCAATGGGA+AGG 0.528993 7.4:+53943980 MS.gene26042:CDS
GAGAGCTTAGTCTTGATCAT+GGG 0.530152 7.4:+53944723 MS.gene26042:CDS
TGTGTTTCTTCAATCATGAA+TGG 0.530317 7.4:+53944164 MS.gene26042:CDS
AGACTAAGCTCTCTCATTGG+AGG 0.531678 7.4:-53944713 None:intergenic
TACATGGTCAACCACAAGCT+GGG 0.533174 7.4:+53943523 MS.gene26042:CDS
AATCTAATCAGAAGATTAGA+GGG 0.539524 7.4:+53943163 MS.gene26042:CDS
ATTGATGTCAGGGTTATTCG+AGG 0.539804 7.4:+53943732 MS.gene26042:CDS
ATAACCACACTAGTAGCAAT+GGG 0.543735 7.4:-53943585 None:intergenic
CATAACCACACTAGTAGCAA+TGG 0.544535 7.4:-53943586 None:intergenic
TATGAAGCAGGATCAGAAGT+TGG 0.550833 7.4:+53943668 MS.gene26042:CDS
TGGGCTATGACTAATATAGC+TGG 0.552213 7.4:-53944603 None:intergenic
GCTACTTTGATGAACGATCA+TGG 0.552325 7.4:+53943402 MS.gene26042:CDS
AGAAGAAGTGAATAATAGTA+GGG 0.557866 7.4:+53943359 MS.gene26042:CDS
TCCAATGCAAAATCTAAAGT+AGG 0.560604 7.4:-53944557 None:intergenic
TGGCCATGGTGATATGAAGC+AGG 0.562913 7.4:+53943656 MS.gene26042:CDS
TTGATCATGGGGTAGAAGCT+GGG 0.563836 7.4:+53944735 MS.gene26042:CDS
ATGTGTGTGATTGGTTTACA+TGG 0.563933 7.4:+53943507 MS.gene26042:CDS
ACGATGGCGCGCAATATGGA+AGG 0.564649 7.4:-53943081 None:intergenic
CTCCGGACCATCTCACGATA+CGG 0.564668 7.4:-53943231 None:intergenic
TTACATGGTCAACCACAAGC+TGG 0.564688 7.4:+53943522 MS.gene26042:CDS
TCATCAGAAAGGACATACAT+AGG 0.567324 7.4:-53943963 None:intergenic
TCACCGTCCGTATCGTGAGA+TGG 0.569169 7.4:+53943224 MS.gene26042:CDS
CTTTGTGGAAGCAAGAACAT+TGG 0.569590 7.4:-53944086 None:intergenic
CATTGGAAATATCATCAGAA+AGG 0.571115 7.4:-53943974 None:intergenic
GGAACGAGGCAACCTTGCTA+TGG 0.574021 7.4:+53943689 MS.gene26042:CDS
TTTCTGATGATATTTCCAAT+GGG 0.576995 7.4:+53943976 MS.gene26042:CDS
GGAGACTCTATTATCTACTC+TGG 0.577576 7.4:+53943636 MS.gene26042:CDS
CCACACTAGTAGCAATGGGC+TGG 0.579492 7.4:-53943581 None:intergenic
GCAATATGAGATTGATGTCA+GGG 0.582106 7.4:+53943722 MS.gene26042:CDS
GGATATAATGATGATGATCA+AGG 0.586899 7.4:+53943615 MS.gene26042:CDS
GGGATATACTCAAATTGCAT+AGG 0.587945 7.4:-53944050 None:intergenic
AAATTACGACAGCAACACTC+TGG 0.588133 7.4:+53943125 MS.gene26042:CDS
TTATAAGTTTATGTTATCAA+GGG 0.591362 7.4:+53943863 MS.gene26042:CDS
GACTAAGCTCTCTCATTGGA+GGG 0.593113 7.4:-53944712 None:intergenic
GCATACTTGTCACCATAGCA+AGG 0.603748 7.4:-53943701 None:intergenic
AAAATCTAAAGTAGGATATG+CGG 0.609459 7.4:-53944549 None:intergenic
GTTCAAGGTCAACCTAAGAT+GGG 0.609539 7.4:+53943885 MS.gene26042:CDS
GGAGCTTGTTCACACTGCGA+TGG 0.614223 7.4:+53943194 MS.gene26042:CDS
AGATCTAATGAAACTAGTTG+GGG 0.616648 7.4:+53944329 MS.gene26042:CDS
TCTATTATCTACTCTGGCCA+TGG 0.620402 7.4:+53943642 MS.gene26042:CDS
TTACGACAGCAACACTCTGG+CGG 0.621013 7.4:+53943128 MS.gene26042:CDS
GAAGATTAGAGGGCAGAACA+AGG 0.625199 7.4:+53943173 MS.gene26042:CDS
CTACCCCTTATAGTAAAAGT+GGG 0.630650 7.4:+53944192 MS.gene26042:CDS
ACACGGTTTCTGCAATGCGA+AGG 0.638201 7.4:-53944269 None:intergenic
AGAGCTTAGTCTTGATCATG+GGG 0.638779 7.4:+53944724 MS.gene26042:CDS
CATTGGATGTATCAACGATG+AGG 0.644772 7.4:+53944573 MS.gene26042:CDS
AAATAATCAATCCCAGCTTG+TGG 0.645273 7.4:-53943534 None:intergenic
ATTGCAAATACCATAAGATG+GGG 0.649831 7.4:-53944680 None:intergenic
CCAGCCCATTGCTACTAGTG+TGG 0.655151 7.4:+53943581 MS.gene26042:CDS
AATAGGTCTACTCAAGAATG+GGG 0.657058 7.4:+53944491 MS.gene26042:CDS
GAAGCCAATATGCTTAAGAG+AGG 0.661393 7.4:+53943924 MS.gene26042:CDS
ATATCACCAATACAAACACC+AGG 0.674752 7.4:-53943465 None:intergenic
GCAATTTGAGTATATCCCGA+AGG 0.675237 7.4:+53944055 MS.gene26042:CDS
CAGGATCAGAAGTTGGAACG+AGG 0.676511 7.4:+53943675 MS.gene26042:CDS
CGATGGCGCGCAATATGGAA+GGG 0.676935 7.4:-53943080 None:intergenic
GGAAGGAGAATATTGGCGTG+AGG 0.681400 7.4:+53943997 MS.gene26042:CDS
GATCCTGCTTCATATCACCA+TGG 0.685147 7.4:-53943659 None:intergenic
GGACCATCTCACGATACGGA+CGG 0.685403 7.4:-53943227 None:intergenic
AGGTCGTTGATTTATGAATG+CGG 0.693955 7.4:+53944230 MS.gene26042:CDS
ATTTGAGTATATCCCGAAGG+CGG 0.717156 7.4:+53944058 MS.gene26042:CDS
TTCCACAAAGTATGACGACG+AGG 0.737459 7.4:+53944099 MS.gene26042:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAGTTTATGTTATCAA+GGG + chr7.4:53943863-53943882 MS.gene26042:CDS 15.0%
!! TTTATAAGTTTATGTTATCA+AGG + chr7.4:53943862-53943881 MS.gene26042:CDS 15.0%
!!! AAAAGGTATTAAATATAGGT+TGG + chr7.4:53944298-53944317 MS.gene26042:CDS 20.0%
!!! CACAAAAAGGTATTAAATAT+AGG + chr7.4:53944294-53944313 MS.gene26042:CDS 20.0%
! AAATCTAATCAGAAGATTAG+AGG + chr7.4:53943162-53943181 MS.gene26042:CDS 25.0%
! AATCTAATCAGAAGATTAGA+GGG + chr7.4:53943163-53943182 MS.gene26042:CDS 25.0%
! ACATAGGATTATCACATAAT+TGG - chr7.4:53943950-53943969 None:intergenic 25.0%
! ACTAATTGAACAAACACATT+TGG - chr7.4:53944632-53944651 None:intergenic 25.0%
! AGAAGAAGTGAATAATAGTA+GGG + chr7.4:53943359-53943378 MS.gene26042:CDS 25.0%
! TAGATCTAATGAAACTAGTT+GGG + chr7.4:53944328-53944347 MS.gene26042:CDS 25.0%
! TATCATGATGTGAAAATCAT+TGG + chr7.4:53943435-53943454 MS.gene26042:CDS 25.0%
! TGTATAAGATAAGTGAATGT+TGG + chr7.4:53943811-53943830 MS.gene26042:CDS 25.0%
! TTAGATCTAATGAAACTAGT+TGG + chr7.4:53944327-53944346 MS.gene26042:CDS 25.0%
! TTTCTGATGATATTTCCAAT+GGG + chr7.4:53943976-53943995 MS.gene26042:CDS 25.0%
!! AAAATCTAAAGTAGGATATG+CGG - chr7.4:53944552-53944571 None:intergenic 25.0%
!!! AGTAAGGTTTTTGTTTATGA+TGG + chr7.4:53943786-53943805 MS.gene26042:CDS 25.0%
!!! ATCCAAATCGATTTTTGAAT+AGG + chr7.4:53944474-53944493 MS.gene26042:CDS 25.0%
AGATCTAATGAAACTAGTTG+GGG + chr7.4:53944329-53944348 MS.gene26042:CDS 30.0%
ATAAAAGTTCCAGCTAGAAT+AGG - chr7.4:53944371-53944390 None:intergenic 30.0%
ATTGCAAATACCATAAGATG+GGG - chr7.4:53944683-53944702 None:intergenic 30.0%
CAAACAAAATTCTTGTTCTC+CGG - chr7.4:53943251-53943270 None:intergenic 30.0%
CAGAAGAAGTGAATAATAGT+AGG + chr7.4:53943358-53943377 MS.gene26042:CDS 30.0%
CATTGCAAATACCATAAGAT+GGG - chr7.4:53944684-53944703 None:intergenic 30.0%
CATTGGAAATATCATCAGAA+AGG - chr7.4:53943977-53943996 None:intergenic 30.0%
CTTTCTGATGATATTTCCAA+TGG + chr7.4:53943975-53943994 MS.gene26042:CDS 30.0%
GACCTATTCAAAAATCGATT+TGG - chr7.4:53944479-53944498 None:intergenic 30.0%
TAAAAGTTCCAGCTAGAATA+GGG - chr7.4:53944370-53944389 None:intergenic 30.0%
TATTATTCACTTCTTCTGTC+TGG - chr7.4:53943356-53943375 None:intergenic 30.0%
TGTGTTTCTTCAATCATGAA+TGG + chr7.4:53944164-53944183 MS.gene26042:CDS 30.0%
TTCTCTTTGATCATGGTAAT+TGG + chr7.4:53944651-53944670 MS.gene26042:CDS 30.0%
TTTATGTTATCAAGGGTTCA+AGG + chr7.4:53943870-53943889 MS.gene26042:CDS 30.0%
! AAAAGTGGGATTCTTTTGAA+AGG + chr7.4:53944206-53944225 MS.gene26042:CDS 30.0%
! AAATCTAAAGTAGGATATGC+GGG - chr7.4:53944551-53944570 None:intergenic 30.0%
! ACTTTTATATGTGAGTTCAC+CGG + chr7.4:53944383-53944402 MS.gene26042:CDS 30.0%
! CAATTAGTTCTCTTTGATCA+TGG + chr7.4:53944644-53944663 MS.gene26042:CDS 30.0%
! GGATATAATGATGATGATCA+AGG + chr7.4:53943615-53943634 MS.gene26042:CDS 30.0%
! GGTTTGAAAAAGGTATATCT+GGG + chr7.4:53943832-53943851 MS.gene26042:CDS 30.0%
! TAATACCTTTTTGTGTCACA+CGG - chr7.4:53944289-53944308 None:intergenic 30.0%
! TCCAATGCAAAATCTAAAGT+AGG - chr7.4:53944560-53944579 None:intergenic 30.0%
! TCCTACTTTAGATTTTGCAT+TGG + chr7.4:53944556-53944575 MS.gene26042:CDS 30.0%
! TGGTTTGAAAAAGGTATATC+TGG + chr7.4:53943831-53943850 MS.gene26042:CDS 30.0%
!! AAAGTGGGATTCTTTTGAAA+GGG + chr7.4:53944207-53944226 MS.gene26042:CDS 30.0%
!! AATCCCACTTTTACTATAAG+GGG - chr7.4:53944198-53944217 None:intergenic 30.0%
!! AGAATCCCACTTTTACTATA+AGG - chr7.4:53944200-53944219 None:intergenic 30.0%
!! AGTGAATGTTGGTTTGAAAA+AGG + chr7.4:53943822-53943841 MS.gene26042:CDS 30.0%
!! GAATCCCACTTTTACTATAA+GGG - chr7.4:53944199-53944218 None:intergenic 30.0%
AAATAATCAATCCCAGCTTG+TGG - chr7.4:53943537-53943556 None:intergenic 35.0%
AATAGGTCTACTCAAGAATG+GGG + chr7.4:53944491-53944510 MS.gene26042:CDS 35.0%
ACTACCCCTTATAGTAAAAG+TGG + chr7.4:53944191-53944210 MS.gene26042:CDS 35.0%
AGAAGTGAAATGTGTGTGAT+TGG + chr7.4:53943498-53943517 MS.gene26042:CDS 35.0%
ATAACCACACTAGTAGCAAT+GGG - chr7.4:53943588-53943607 None:intergenic 35.0%
ATATCACCAATACAAACACC+AGG - chr7.4:53943468-53943487 None:intergenic 35.0%
CCATCTTATGGTATTTGCAA+TGG + chr7.4:53944682-53944701 MS.gene26042:CDS 35.0%
CCATTGCAAATACCATAAGA+TGG - chr7.4:53944685-53944704 None:intergenic 35.0%
CTACCCCTTATAGTAAAAGT+GGG + chr7.4:53944192-53944211 MS.gene26042:CDS 35.0%
GAATAGGTCTACTCAAGAAT+GGG + chr7.4:53944490-53944509 MS.gene26042:CDS 35.0%
GCAATATGAGATTGATGTCA+GGG + chr7.4:53943722-53943741 MS.gene26042:CDS 35.0%
GGGATATACTCAAATTGCAT+AGG - chr7.4:53944053-53944072 None:intergenic 35.0%
TAGCAGCAATCAAGTCATTT+CGG - chr7.4:53943386-53943405 None:intergenic 35.0%
TCATCAGAAAGGACATACAT+AGG - chr7.4:53943966-53943985 None:intergenic 35.0%
TGAATAGGTCTACTCAAGAA+TGG + chr7.4:53944489-53944508 MS.gene26042:CDS 35.0%
TGATGATATTTCCAATGGGA+AGG + chr7.4:53943980-53943999 MS.gene26042:CDS 35.0%
TGCAATATGAGATTGATGTC+AGG + chr7.4:53943721-53943740 MS.gene26042:CDS 35.0%
TGGTACTTCTACTACTAGTA+AGG + chr7.4:53943770-53943789 MS.gene26042:CDS 35.0%
! CATGCTAGTATGAGTTCTAA+TGG + chr7.4:53943558-53943577 MS.gene26042:CDS 35.0%
! GAAAAAGGTATATCTGGGTT+TGG + chr7.4:53943837-53943856 MS.gene26042:CDS 35.0%
! GAACAAACACATTTGGAGTT+GGG - chr7.4:53944625-53944644 None:intergenic 35.0%
! GTCGTAATTTTGTAACTCGT+CGG - chr7.4:53943115-53943134 None:intergenic 35.0%
! TCGTAATTTTGTAACTCGTC+GGG - chr7.4:53943114-53943133 None:intergenic 35.0%
! TGAACAAACACATTTGGAGT+TGG - chr7.4:53944626-53944645 None:intergenic 35.0%
!! AGGTCGTTGATTTATGAATG+CGG + chr7.4:53944230-53944249 MS.gene26042:CDS 35.0%
!! ATGTGTGTGATTGGTTTACA+TGG + chr7.4:53943507-53943526 MS.gene26042:CDS 35.0%
!! TAAGCATATTGGCTTCCTTA+AGG - chr7.4:53943920-53943939 None:intergenic 35.0%
AAAATCATTGGTCCAGTTCC+TGG + chr7.4:53943447-53943466 MS.gene26042:CDS 40.0%
AAATTACGACAGCAACACTC+TGG + chr7.4:53943125-53943144 MS.gene26042:CDS 40.0%
ACTAGTGTGGTTATGTCTGA+TGG + chr7.4:53943594-53943613 MS.gene26042:CDS 40.0%
AGAAACCGTGTGACACAAAA+AGG + chr7.4:53944281-53944300 MS.gene26042:CDS 40.0%
AGAGTTATGTTCACCATCCA+TGG - chr7.4:53944444-53944463 None:intergenic 40.0%
AGGATCATACTACCCATCTT+AGG - chr7.4:53943900-53943919 None:intergenic 40.0%
ATGAAACTAGTTGGGGAGTT+AGG + chr7.4:53944336-53944355 MS.gene26042:CDS 40.0%
ATTGATGTCAGGGTTATTCG+AGG + chr7.4:53943732-53943751 MS.gene26042:CDS 40.0%
ATTTGAGTATATCCCGAAGG+CGG + chr7.4:53944058-53944077 MS.gene26042:CDS 40.0%
CATAACCACACTAGTAGCAA+TGG - chr7.4:53943589-53943608 None:intergenic 40.0%
CATTGGATGTATCAACGATG+AGG + chr7.4:53944573-53944592 MS.gene26042:CDS 40.0%
CTTTGTGGAAGCAAGAACAT+TGG - chr7.4:53944089-53944108 None:intergenic 40.0%
GAAGCCAATATGCTTAAGAG+AGG + chr7.4:53943924-53943943 MS.gene26042:CDS 40.0%
GATGGGTAGTATGATCCTTA+AGG + chr7.4:53943902-53943921 MS.gene26042:CDS 40.0%
GCAATTTGAGTATATCCCGA+AGG + chr7.4:53944055-53944074 MS.gene26042:CDS 40.0%
GGAGACTCTATTATCTACTC+TGG + chr7.4:53943636-53943655 MS.gene26042:CDS 40.0%
GTTCAAGGTCAACCTAAGAT+GGG + chr7.4:53943885-53943904 MS.gene26042:CDS 40.0%
TATGAAGCAGGATCAGAAGT+TGG + chr7.4:53943668-53943687 MS.gene26042:CDS 40.0%
TCAAGACTAAGCTCTCTCAT+TGG - chr7.4:53944719-53944738 None:intergenic 40.0%
TCCAATGGGAAGGAGAATAT+TGG + chr7.4:53943990-53944009 MS.gene26042:CDS 40.0%
TCTATTATCTACTCTGGCCA+TGG + chr7.4:53943642-53943661 MS.gene26042:CDS 40.0%
TGCGAAGGACAACTACAAAA+AGG - chr7.4:53944257-53944276 None:intergenic 40.0%
TGGGCTATGACTAATATAGC+TGG - chr7.4:53944606-53944625 None:intergenic 40.0%
TTGGATGTTTCCCCATCTTA+TGG + chr7.4:53944670-53944689 MS.gene26042:CDS 40.0%
TTGGCCTCTCTTAAGCATAT+TGG - chr7.4:53943931-53943950 None:intergenic 40.0%
! ATTCGAGGTTTTCGGTATGA+AGG + chr7.4:53943747-53943766 MS.gene26042:CDS 40.0%
! GCTACTTTGATGAACGATCA+TGG + chr7.4:53943402-53943421 MS.gene26042:CDS 40.0%
! GTTTGATTCGATTCGTGTGT+CGG + chr7.4:53943266-53943285 MS.gene26042:CDS 40.0%
! TCAGGGTTATTCGAGGTTTT+CGG + chr7.4:53943739-53943758 MS.gene26042:CDS 40.0%
! TTTGATTCGATTCGTGTGTC+GGG + chr7.4:53943267-53943286 MS.gene26042:CDS 40.0%
!! AGAGAGCTTAGTCTTGATCA+TGG + chr7.4:53944722-53944741 MS.gene26042:CDS 40.0%
!! AGAGCTTAGTCTTGATCATG+GGG + chr7.4:53944724-53944743 MS.gene26042:CDS 40.0%
!! GAGAGCTTAGTCTTGATCAT+GGG + chr7.4:53944723-53944742 MS.gene26042:CDS 40.0%
!! GCAAGCAGAAATTTTGACCA+TGG + chr7.4:53944424-53944443 MS.gene26042:CDS 40.0%
!! GCAGAAATTTTGACCATGGA+TGG + chr7.4:53944428-53944447 MS.gene26042:CDS 40.0%
AACATTGGAAATGCCGCCTT+CGG - chr7.4:53944074-53944093 None:intergenic 45.0%
AGACTAAGCTCTCTCATTGG+AGG - chr7.4:53944716-53944735 None:intergenic 45.0%
CCAATACAAACACCAGGAAC+TGG - chr7.4:53943462-53943481 None:intergenic 45.0%
CCAGTTCCTGGTGTTTGTAT+TGG + chr7.4:53943459-53943478 MS.gene26042:CDS 45.0%
CTCCCTAGTCAAAACAAGTC+CGG - chr7.4:53944405-53944424 None:intergenic 45.0%
GAAGATTAGAGGGCAGAACA+AGG + chr7.4:53943173-53943192 MS.gene26042:CDS 45.0%
GACTAAGCTCTCTCATTGGA+GGG - chr7.4:53944715-53944734 None:intergenic 45.0%
GATCCTGCTTCATATCACCA+TGG - chr7.4:53943662-53943681 None:intergenic 45.0%
GCATACTTGTCACCATAGCA+AGG - chr7.4:53943704-53943723 None:intergenic 45.0%
GCCAATATTCTCCTTCCCAT+TGG - chr7.4:53943994-53944013 None:intergenic 45.0%
GGTTCAAGGTCAACCTAAGA+TGG + chr7.4:53943884-53943903 MS.gene26042:CDS 45.0%
TACATGGTCAACCACAAGCT+GGG + chr7.4:53943523-53943542 MS.gene26042:CDS 45.0%
TAGTTGGGGAGTTAGGTCTT+TGG + chr7.4:53944343-53944362 MS.gene26042:CDS 45.0%
TCTTTGGACCCTATTCTAGC+TGG + chr7.4:53944359-53944378 MS.gene26042:CDS 45.0%
TTACATGGTCAACCACAAGC+TGG + chr7.4:53943522-53943541 MS.gene26042:CDS 45.0%
TTCCACAAAGTATGACGACG+AGG + chr7.4:53944099-53944118 MS.gene26042:CDS 45.0%
TTCCTCGTCGTCATACTTTG+TGG - chr7.4:53944104-53944123 None:intergenic 45.0%
TTGATCATGGGGTAGAAGCT+GGG + chr7.4:53944735-53944754 MS.gene26042:CDS 45.0%
! GAGTGTTCTGAATGCGTTGA+TGG + chr7.4:53944140-53944159 MS.gene26042:CDS 45.0%
!! GTGGGATTCTTTTGAAAGGG+AGG + chr7.4:53944210-53944229 MS.gene26042:CDS 45.0%
!!! CACCGGACTTGTTTTGACTA+GGG + chr7.4:53944400-53944419 MS.gene26042:CDS 45.0%
!!! TCACCGGACTTGTTTTGACT+AGG + chr7.4:53944399-53944418 MS.gene26042:CDS 45.0%
ACACGGTTTCTGCAATGCGA+AGG - chr7.4:53944272-53944291 None:intergenic 50.0%
ACATTGGAAATGCCGCCTTC+GGG - chr7.4:53944073-53944092 None:intergenic 50.0%
CAGGATCAGAAGTTGGAACG+AGG + chr7.4:53943675-53943694 MS.gene26042:CDS 50.0%
CTTGATCATGGGGTAGAAGC+TGG + chr7.4:53944734-53944753 MS.gene26042:CDS 50.0%
GGAAGGAGAATATTGGCGTG+AGG + chr7.4:53943997-53944016 MS.gene26042:CDS 50.0%
TGGCCATGGTGATATGAAGC+AGG + chr7.4:53943656-53943675 MS.gene26042:CDS 50.0%
TTACGACAGCAACACTCTGG+CGG + chr7.4:53943128-53943147 MS.gene26042:CDS 50.0%
! CGAGGTTTTCGGTATGAAGG+TGG + chr7.4:53943750-53943769 MS.gene26042:CDS 50.0%
! TTTTGTAACTCGTCGGGACC+TGG - chr7.4:53943108-53943127 None:intergenic 50.0%
ACGATGGCGCGCAATATGGA+AGG - chr7.4:53943084-53943103 None:intergenic 55.0%
ATATTGCGCGCCATCGTTCC+AGG + chr7.4:53943087-53943106 MS.gene26042:CDS 55.0%
CCACACTAGTAGCAATGGGC+TGG - chr7.4:53943584-53943603 None:intergenic 55.0%
CCAGCCCATTGCTACTAGTG+TGG + chr7.4:53943581-53943600 MS.gene26042:CDS 55.0%
CGATGGCGCGCAATATGGAA+GGG - chr7.4:53943083-53943102 None:intergenic 55.0%
CTCCGGACCATCTCACGATA+CGG - chr7.4:53943234-53943253 None:intergenic 55.0%
GATTCGTGTGTCGGGTTTGC+TGG + chr7.4:53943275-53943294 MS.gene26042:CDS 55.0%
GCGCGCAATATGGAAGGGTT+TGG - chr7.4:53943078-53943097 None:intergenic 55.0%
GGAACGAGGCAACCTTGCTA+TGG + chr7.4:53943689-53943708 MS.gene26042:CDS 55.0%
GGACCATCTCACGATACGGA+CGG - chr7.4:53943230-53943249 None:intergenic 55.0%
GTCCGTATCGTGAGATGGTC+CGG + chr7.4:53943229-53943248 MS.gene26042:CDS 55.0%
TACGACAGCAACACTCTGGC+GGG + chr7.4:53943129-53943148 MS.gene26042:CDS 55.0%
TCACCGTCCGTATCGTGAGA+TGG + chr7.4:53943224-53943243 MS.gene26042:CDS 55.0%
TGGAACGATGGCGCGCAATA+TGG - chr7.4:53943088-53943107 None:intergenic 55.0%
! GGAGCTTGTTCACACTGCGA+TGG + chr7.4:53943194-53943213 MS.gene26042:CDS 55.0%
CTCGTCGGGACCTGGAACGA+TGG - chr7.4:53943100-53943119 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 53943060 53944760 53943060 ID=MS.gene26042
chr7.4 mRNA 53943060 53944760 53943060 ID=MS.gene26042.t1;Parent=MS.gene26042
chr7.4 exon 53943060 53944760 53943060 ID=MS.gene26042.t1.exon1;Parent=MS.gene26042.t1
chr7.4 CDS 53943060 53944760 53943060 ID=cds.MS.gene26042.t1;Parent=MS.gene26042.t1
Gene Sequence

>MS.gene26042

ATGAATTTTCAAGATCCAAACCCTTCCATATTGCGCGCCATCGTTCCAGGTCCCGACGAGTTACAAAATTACGACAGCAACACTCTGGCGGGAAAAGCTTCAAAATCTAATCAGAAGATTAGAGGGCAGAACAAGGAGCTTGTTCACACTGCGATGGATGCTGATCACCGTCCGTATCGTGAGATGGTCCGGAGAACAAGAATTTTGTTTGATTCGATTCGTGTGTCGGGTTTGCTGGAAGAAGAGAATAATCGTAAGAACAAGAAGTATTTGAAAACTTCTTCTTTGATAAACCAGACAGAAGAAGTGAATAATAGTAGGGTCCGAAATGACTTGATTGCTGCTACTTTGATGAACGATCATGGTATGTATTTGTATCATGATGTGAAAATCATTGGTCCAGTTCCTGGTGTTTGTATTGGTGATATTTTTCTCTATAGAAGTGAAATGTGTGTGATTGGTTTACATGGTCAACCACAAGCTGGGATTGATTATTTACATGCTAGTATGAGTTCTAATGGCCAGCCCATTGCTACTAGTGTGGTTATGTCTGATGGATATAATGATGATGATCAAGGAGACTCTATTATCTACTCTGGCCATGGTGATATGAAGCAGGATCAGAAGTTGGAACGAGGCAACCTTGCTATGGTGACAAGTATGCAATATGAGATTGATGTCAGGGTTATTCGAGGTTTTCGGTATGAAGGTGGTACTTCTACTACTAGTAAGGTTTTTGTTTATGATGGCTTGTATAAGATAAGTGAATGTTGGTTTGAAAAAGGTATATCTGGGTTTGGTGTTTATAAGTTTATGTTATCAAGGGTTCAAGGTCAACCTAAGATGGGTAGTATGATCCTTAAGGAAGCCAATATGCTTAAGAGAGGCCAATTATGTGATAATCCTATGTATGTCCTTTCTGATGATATTTCCAATGGGAAGGAGAATATTGGCGTGAGGCTATATAATGACATTGATAGTGATCTCTATCCTATGCAATTTGAGTATATCCCGAAGGCGGCATTTCCAATGTTCTTGCTTCCACAAAGTATGACGACGAGGAAAAAGATGAGAGCGATAGAGTGTTCTGAATGCGTTGATGGATGTGTTTCTTCAATCATGAATGGTAACACTACCCCTTATAGTAAAAGTGGGATTCTTTTGAAAGGGAGGTCGTTGATTTATGAATGCGGTCCTTTTTGTAGTTGTCCTTCGCATTGCAGAAACCGTGTGACACAAAAAGGTATTAAATATAGGTTGGAAGTGTTTAGATCTAATGAAACTAGTTGGGGAGTTAGGTCTTTGGACCCTATTCTAGCTGGAACTTTTATATGTGAGTTCACCGGACTTGTTTTGACTAGGGAGCAAGCAGAAATTTTGACCATGGATGGTGAACATAACTCTTTGATTATATATCCAAATCGATTTTTGAATAGGTCTACTCAAGAATGGGGAGATTTGTCGATGATTGATGTTAATCATGTGCATCCCGCATATCCTACTTTAGATTTTGCATTGGATGTATCAACGATGAGGAATGTTGCCAGCTATATTAGTCATAGCCCAACTCCAAATGTGTTTGTTCAATTAGTTCTCTTTGATCATGGTAATTGGATGTTTCCCCATCTTATGGTATTTGCAATGGAAAACATCCCTCCAATGAGAGAGCTTAGTCTTGATCATGGGGTAGAAGCTGGGTAG
Protein sequence

>MS.gene26042.t1

MNFQDPNPSILRAIVPGPDELQNYDSNTLAGKASKSNQKIRGQNKELVHTAMDADHRPYREMVRRTRILFDSIRVSGLLEEENNRKNKKYLKTSSLINQTEEVNNSRVRNDLIAATLMNDHGMYLYHDVKIIGPVPGVCIGDIFLYRSEMCVIGLHGQPQAGIDYLHASMSSNGQPIATSVVMSDGYNDDDQGDSIIYSGHGDMKQDQKLERGNLAMVTSMQYEIDVRVIRGFRYEGGTSTTSKVFVYDGLYKISECWFEKGISGFGVYKFMLSRVQGQPKMGSMILKEANMLKRGQLCDNPMYVLSDDISNGKENIGVRLYNDIDSDLYPMQFEYIPKAAFPMFLLPQSMTTRKKMRAIECSECVDGCVSSIMNGNTTPYSKSGILLKGRSLIYECGPFCSCPSHCRNRVTQKGIKYRLEVFRSNETSWGVRSLDPILAGTFICEFTGLVLTREQAEILTMDGEHNSLIIYPNRFLNRSTQEWGDLSMIDVNHVHPAYPTLDFALDVSTMRNVASYISHSPTPNVFVQLVLFDHGNWMFPHLMVFAMENIPPMRELSLDHGVEAG