Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26136.t1 | RHN56887.1 | 83.5 | 176 | 17 | 1 | 1 | 164 | 32 | 207 | 2.40E-80 | 308.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26136.t1 | G7KG15 | 83.5 | 176 | 17 | 1 | 1 | 164 | 12 | 187 | 1.7e-80 | 308.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene26136.t1 | TF | PLATZ |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26136.t1 | MTR_5g077740 | 83.523 | 176 | 17 | 1 | 1 | 164 | 12 | 187 | 6.98e-109 | 308 |
| MS.gene26136.t1 | MTR_8g097310 | 39.073 | 151 | 69 | 3 | 37 | 164 | 4 | 154 | 6.69e-30 | 107 |
| MS.gene26136.t1 | MTR_1g062160 | 27.000 | 200 | 93 | 6 | 12 | 163 | 7 | 201 | 5.44e-15 | 69.7 |
| MS.gene26136.t1 | MTR_4g094680 | 28.378 | 148 | 75 | 4 | 11 | 134 | 21 | 161 | 4.57e-12 | 62.4 |
| MS.gene26136.t1 | MTR_7g102790 | 25.743 | 202 | 100 | 7 | 9 | 163 | 4 | 202 | 8.15e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26136.t1 | AT2G27930 | 26.738 | 187 | 105 | 4 | 9 | 163 | 1 | 187 | 4.28e-14 | 67.0 |
| MS.gene26136.t1 | AT2G27930 | 26.738 | 187 | 105 | 4 | 9 | 163 | 1 | 187 | 4.28e-14 | 67.0 |
| MS.gene26136.t1 | AT1G31040 | 28.261 | 138 | 83 | 3 | 11 | 135 | 18 | 152 | 7.17e-12 | 62.0 |
| MS.gene26136.t1 | AT1G43000 | 25.463 | 216 | 97 | 8 | 8 | 163 | 12 | 223 | 2.10e-11 | 60.5 |
| MS.gene26136.t1 | AT1G43000 | 25.463 | 216 | 97 | 8 | 8 | 163 | 5 | 216 | 2.28e-11 | 60.1 |
| MS.gene26136.t1 | AT1G32700 | 23.383 | 201 | 99 | 7 | 12 | 163 | 12 | 206 | 2.96e-11 | 59.7 |
Find 35 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTATAAACTTTGAGTATC+TGG | 0.212381 | 5.4:+57077626 | None:intergenic |
| TATCAACTGCTCCAAGATTA+AGG | 0.334487 | 5.4:-57077560 | MS.gene26136:intron |
| TCACCAGCCAAAGTGGCTTC+AGG | 0.350529 | 5.4:-57077782 | MS.gene26136:CDS |
| GACACATCATCATGCGGCTT+TGG | 0.388033 | 5.4:-57076895 | MS.gene26136:CDS |
| ATTGCAAAGAATCTGCTTGC+CGG | 0.399091 | 5.4:-57077681 | MS.gene26136:CDS |
| ATAGTAGAATACCTTAATCT+TGG | 0.415338 | 5.4:+57077549 | None:intergenic |
| AATCCTGAAGCCACTTTGGC+TGG | 0.443360 | 5.4:+57077779 | None:intergenic |
| AAAGAATCTGCTTGCCGGTA+TGG | 0.452183 | 5.4:-57077676 | MS.gene26136:CDS |
| TGGCTGGTGAACTTCACGTT+GGG | 0.459890 | 5.4:+57077795 | None:intergenic |
| ACTTTCTTTGATCAGAAGAA+AGG | 0.463053 | 5.4:+57076597 | None:intergenic |
| TGCCGGTATGGTCTATCATC+AGG | 0.465550 | 5.4:-57077664 | MS.gene26136:CDS |
| CTGAAGATTCAATTCCTACT+CGG | 0.475908 | 5.4:-57076683 | MS.gene26136:CDS |
| TATATGACTGCATAGTAGTA+AGG | 0.513325 | 5.4:+57077581 | None:intergenic |
| GTACTGCTCCATTGCTTGCA+AGG | 0.516727 | 5.4:-57076839 | MS.gene26136:intron |
| TTTGGCAGCAGAAACTCAAA+TGG | 0.521805 | 5.4:-57076877 | MS.gene26136:CDS |
| CCCACGATGTGGATCAGCAT+TGG | 0.527266 | 5.4:-57076920 | MS.gene26136:CDS |
| TTGGCTGGTGAACTTCACGT+TGG | 0.528347 | 5.4:+57077794 | None:intergenic |
| TCCCCTTGTGATTCCCGAGT+AGG | 0.539017 | 5.4:+57076669 | None:intergenic |
| TGAAGATTCAATTCCTACTC+GGG | 0.557663 | 5.4:-57076682 | MS.gene26136:CDS |
| GTGAAGTTCACCAGCCAAAG+TGG | 0.592642 | 5.4:-57077789 | MS.gene26136:CDS |
| AGTCATCACCTTGCAAGCAA+TGG | 0.595069 | 5.4:+57076831 | None:intergenic |
| ATTCCTACTCGGGAATCACA+AGG | 0.595771 | 5.4:-57076672 | MS.gene26136:CDS |
| CTGCATCGTCTCCCACGATG+TGG | 0.599916 | 5.4:-57076931 | MS.gene26136:CDS |
| ATACAAATGCAACAACCAGC+GGG | 0.605757 | 5.4:-57076956 | MS.gene26136:CDS |
| GGAGACGATGCAGAACCCGC+TGG | 0.607272 | 5.4:+57076941 | None:intergenic |
| TTCCTACTCGGGAATCACAA+GGG | 0.613593 | 5.4:-57076671 | MS.gene26136:CDS |
| TCCTACTCGGGAATCACAAG+GGG | 0.620881 | 5.4:-57076670 | MS.gene26136:CDS |
| TAATTTGTAGGATGAGCAAG+TGG | 0.626865 | 5.4:-57077818 | None:intergenic |
| AATACAAATGCAACAACCAG+CGG | 0.631937 | 5.4:-57076957 | MS.gene26136:CDS |
| CCAATGCTGATCCACATCGT+GGG | 0.635092 | 5.4:+57076920 | None:intergenic |
| TCCAATGCTGATCCACATCG+TGG | 0.660274 | 5.4:+57076919 | None:intergenic |
| TTGGATGACACATCATCATG+CGG | 0.687565 | 5.4:-57076901 | MS.gene26136:CDS |
| AGTTTATAAATATATGCACA+AGG | 0.697960 | 5.4:-57077614 | MS.gene26136:CDS |
| ATCCTGATGATAGACCATAC+CGG | 0.703440 | 5.4:+57077662 | None:intergenic |
| CAGAAGAAAGGAGCTCTGTG+TGG | 0.766323 | 5.4:+57076609 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATTTGATATAGTTTA+TGG | - | chr5.4:57077181-57077200 | MS.gene26136:intron | 10.0% |
| !!! | ATAATTGTAGTAAGAATTTT+AGG | + | chr5.4:57077345-57077364 | None:intergenic | 15.0% |
| !!! | TTTTTTTTATTTGAACTTTC+AGG | - | chr5.4:57077689-57077708 | MS.gene26136:CDS | 15.0% |
| !! | AGTTTATAAATATATGCACA+AGG | - | chr5.4:57076802-57076821 | MS.gene26136:intron | 20.0% |
| !! | ATTTATAAACTTTGAGTATC+TGG | + | chr5.4:57076793-57076812 | None:intergenic | 20.0% |
| !!! | ATGTTTTGCTAATTGTTAAT+TGG | - | chr5.4:57077234-57077253 | MS.gene26136:intron | 20.0% |
| ! | ACAATTAGCAAAACATGTAT+TGG | + | chr5.4:57077230-57077249 | None:intergenic | 25.0% |
| ! | AGAGAGTTATAGTAGATATT+GGG | + | chr5.4:57077292-57077311 | None:intergenic | 25.0% |
| ! | ATAGTAGAATACCTTAATCT+TGG | + | chr5.4:57076870-57076889 | None:intergenic | 25.0% |
| ! | TAGAGAGTTATAGTAGATAT+TGG | + | chr5.4:57077293-57077312 | None:intergenic | 25.0% |
| ! | TAGATATTTCTCTGTTAAAG+TGG | - | chr5.4:57077151-57077170 | MS.gene26136:intron | 25.0% |
| ! | TTACTACAATTATGTTTGCA+TGG | - | chr5.4:57077352-57077371 | MS.gene26136:intron | 25.0% |
| !!! | AGTAAGAATTTTAGGTGTAT+GGG | + | chr5.4:57077337-57077356 | None:intergenic | 25.0% |
| !!! | TAGTAAGAATTTTAGGTGTA+TGG | + | chr5.4:57077338-57077357 | None:intergenic | 25.0% |
| ATATTTCTCTGTTAAAGTGG+TGG | - | chr5.4:57077154-57077173 | MS.gene26136:intron | 30.0% | |
| ATCTACTATAACTCTCTAAG+TGG | - | chr5.4:57077295-57077314 | MS.gene26136:intron | 30.0% | |
| GCATGGAAGAGTAAATAAAT+GGG | - | chr5.4:57077369-57077388 | MS.gene26136:intron | 30.0% | |
| TAATGAACGGGAACAATAAA+TGG | + | chr5.4:57077629-57077648 | None:intergenic | 30.0% | |
| TATATGACTGCATAGTAGTA+AGG | + | chr5.4:57076838-57076857 | None:intergenic | 30.0% | |
| TGCATGGAAGAGTAAATAAA+TGG | - | chr5.4:57077368-57077387 | MS.gene26136:intron | 30.0% | |
| ! | ATGTAACAAGAAAGCATAGT+AGG | - | chr5.4:57077066-57077085 | MS.gene26136:intron | 30.0% |
| ! | TTTCCTTTTAGATGTGTTGT+TGG | - | chr5.4:57077114-57077133 | MS.gene26136:intron | 30.0% |
| !! | TTCCTTTTAGATGTGTTGTT+GGG | - | chr5.4:57077115-57077134 | MS.gene26136:intron | 30.0% |
| AAAAAATCCTGAAGCCACTT+TGG | + | chr5.4:57076644-57076663 | None:intergenic | 35.0% | |
| AAACTTCTGAGCCTCAAAAA+AGG | - | chr5.4:57077769-57077788 | MS.gene26136:CDS | 35.0% | |
| AACTCACAAGTGTAATGAAC+GGG | + | chr5.4:57077641-57077660 | None:intergenic | 35.0% | |
| AATACAAATGCAACAACCAG+CGG | - | chr5.4:57077459-57077478 | MS.gene26136:intron | 35.0% | |
| CCTCAAAAAAGGAAACGAAA+AGG | - | chr5.4:57077780-57077799 | MS.gene26136:CDS | 35.0% | |
| CTGAAGATTCAATTCCTACT+CGG | - | chr5.4:57077733-57077752 | MS.gene26136:intron | 35.0% | |
| GAACTCACAAGTGTAATGAA+CGG | + | chr5.4:57077642-57077661 | None:intergenic | 35.0% | |
| TATCAACTGCTCCAAGATTA+AGG | - | chr5.4:57076856-57076875 | MS.gene26136:CDS | 35.0% | |
| TGAAGATTCAATTCCTACTC+GGG | - | chr5.4:57077734-57077753 | MS.gene26136:intron | 35.0% | |
| TGACTTTGGTTGCTAACAAT+AGG | - | chr5.4:57076979-57076998 | MS.gene26136:intron | 35.0% | |
| !! | AGAATTTTAGGTGTATGGGT+AGG | + | chr5.4:57077333-57077352 | None:intergenic | 35.0% |
| !! | CCTTTTCGTTTCCTTTTTTG+AGG | + | chr5.4:57077783-57077802 | None:intergenic | 35.0% |
| !! | GAATTTTAGGTGTATGGGTA+GGG | + | chr5.4:57077332-57077351 | None:intergenic | 35.0% |
| !! | TTCTGTGCTTTTTGTGACTT+TGG | - | chr5.4:57076965-57076984 | MS.gene26136:intron | 35.0% |
| !!! | AAAGTGGCTTCAGGATTTTT+TGG | - | chr5.4:57076643-57076662 | MS.gene26136:CDS | 35.0% |
| !!! | AAGTGGCTTCAGGATTTTTT+GGG | - | chr5.4:57076644-57076663 | MS.gene26136:CDS | 35.0% |
| ATACAAATGCAACAACCAGC+GGG | - | chr5.4:57077460-57077479 | MS.gene26136:intron | 40.0% | |
| ATCCTGATGATAGACCATAC+CGG | + | chr5.4:57076757-57076776 | None:intergenic | 40.0% | |
| ATTGCAAAGAATCTGCTTGC+CGG | - | chr5.4:57076735-57076754 | MS.gene26136:intron | 40.0% | |
| CACCCAACAACACATCTAAA+AGG | + | chr5.4:57077120-57077139 | None:intergenic | 40.0% | |
| TTCTCTGTTAAAGTGGTGGT+AGG | - | chr5.4:57077158-57077177 | MS.gene26136:intron | 40.0% | |
| TTGGATGACACATCATCATG+CGG | - | chr5.4:57077515-57077534 | MS.gene26136:intron | 40.0% | |
| TTGTATTGCTGCGATCAAAG+AGG | + | chr5.4:57077446-57077465 | None:intergenic | 40.0% | |
| TTTGGCAGCAGAAACTCAAA+TGG | - | chr5.4:57077539-57077558 | MS.gene26136:intron | 40.0% | |
| !!! | AGTGGCTTCAGGATTTTTTG+GGG | - | chr5.4:57076645-57076664 | MS.gene26136:CDS | 40.0% |
| AAAGAATCTGCTTGCCGGTA+TGG | - | chr5.4:57076740-57076759 | MS.gene26136:intron | 45.0% | |
| AGTCATCACCTTGCAAGCAA+TGG | + | chr5.4:57077588-57077607 | None:intergenic | 45.0% | |
| ATGGACATTCAGTGCAGCAT+TGG | - | chr5.4:57077200-57077219 | MS.gene26136:intron | 45.0% | |
| ATTCCTACTCGGGAATCACA+AGG | - | chr5.4:57077744-57077763 | MS.gene26136:intron | 45.0% | |
| GTTCTGCTCATTCAGATCGA+AGG | - | chr5.4:57076687-57076706 | MS.gene26136:CDS | 45.0% | |
| TTCCTACTCGGGAATCACAA+GGG | - | chr5.4:57077745-57077764 | MS.gene26136:intron | 45.0% | |
| CAGAAGAAAGGAGCTCTGTG+TGG | + | chr5.4:57077810-57077829 | None:intergenic | 50.0% | |
| CCAATGCTGATCCACATCGT+GGG | + | chr5.4:57077499-57077518 | None:intergenic | 50.0% | |
| GACACATCATCATGCGGCTT+TGG | - | chr5.4:57077521-57077540 | MS.gene26136:intron | 50.0% | |
| GTACTGCTCCATTGCTTGCA+AGG | - | chr5.4:57077577-57077596 | MS.gene26136:CDS | 50.0% | |
| GTGAAGTTCACCAGCCAAAG+TGG | - | chr5.4:57076627-57076646 | MS.gene26136:CDS | 50.0% | |
| TCCAATGCTGATCCACATCG+TGG | + | chr5.4:57077500-57077519 | None:intergenic | 50.0% | |
| TCCTACTCGGGAATCACAAG+GGG | - | chr5.4:57077746-57077765 | MS.gene26136:intron | 50.0% | |
| TGCCGGTATGGTCTATCATC+AGG | - | chr5.4:57076752-57076771 | MS.gene26136:intron | 50.0% | |
| TGGCTGGTGAACTTCACGTT+GGG | + | chr5.4:57076624-57076643 | None:intergenic | 50.0% | |
| TTGGCTGGTGAACTTCACGT+TGG | + | chr5.4:57076625-57076644 | None:intergenic | 50.0% | |
| ! | AATCCTGAAGCCACTTTGGC+TGG | + | chr5.4:57076640-57076659 | None:intergenic | 50.0% |
| CCCACGATGTGGATCAGCAT+TGG | - | chr5.4:57077496-57077515 | MS.gene26136:intron | 55.0% | |
| TCACCAGCCAAAGTGGCTTC+AGG | - | chr5.4:57076634-57076653 | MS.gene26136:CDS | 55.0% | |
| TCCCCTTGTGATTCCCGAGT+AGG | + | chr5.4:57077750-57077769 | None:intergenic | 55.0% | |
| CTGCATCGTCTCCCACGATG+TGG | - | chr5.4:57077485-57077504 | MS.gene26136:intron | 60.0% | |
| GGAGACGATGCAGAACCCGC+TGG | + | chr5.4:57077478-57077497 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 57076608 | 57077830 | 57076608 | ID=MS.gene26136 |
| chr5.4 | mRNA | 57076608 | 57077830 | 57076608 | ID=MS.gene26136.t1;Parent=MS.gene26136 |
| chr5.4 | exon | 57077747 | 57077830 | 57077747 | ID=MS.gene26136.t1.exon1;Parent=MS.gene26136.t1 |
| chr5.4 | CDS | 57077747 | 57077830 | 57077747 | ID=cds.MS.gene26136.t1;Parent=MS.gene26136.t1 |
| chr5.4 | exon | 57077561 | 57077710 | 57077561 | ID=MS.gene26136.t1.exon2;Parent=MS.gene26136.t1 |
| chr5.4 | CDS | 57077561 | 57077710 | 57077561 | ID=cds.MS.gene26136.t1;Parent=MS.gene26136.t1 |
| chr5.4 | exon | 57076840 | 57076980 | 57076840 | ID=MS.gene26136.t1.exon3;Parent=MS.gene26136.t1 |
| chr5.4 | CDS | 57076840 | 57076980 | 57076840 | ID=cds.MS.gene26136.t1;Parent=MS.gene26136.t1 |
| chr5.4 | exon | 57076608 | 57076727 | 57076608 | ID=MS.gene26136.t1.exon4;Parent=MS.gene26136.t1 |
| chr5.4 | CDS | 57076608 | 57076727 | 57076608 | ID=cds.MS.gene26136.t1;Parent=MS.gene26136.t1 |
| Gene Sequence |
| Protein sequence |
