AlfalfaGEDB | MS.gene26261

M. sativa cultivar XinJiangDaYe / MS.gene26261

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26261.t1	XP_003616031.1	91.4	162	14	0	1	162	1	162	1.30E-78	302.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26261.t1	Q9FJK3	38.6	153	94	0	1	153	1	153	3.4e-27	122.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26261.t1	G7K0T4	91.4	162	14	0	1	162	1	162	9.3e-79	302.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene26261.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26261.t1	MTR_5g075380	91.358	162	14	0	1	162	1	162	2.31e-112	316
MS.gene26261.t1	MTR_3g466980	41.875	160	93	0	1	160	1	160	1.22e-44	146
MS.gene26261.t1	MTR_4g032620	43.506	154	86	1	1	153	1	154	1.09e-41	139
MS.gene26261.t1	MTR_3g467080	43.506	154	86	1	1	153	1	154	1.57e-40	134
MS.gene26261.t1	MTR_4g031910	45.455	154	83	1	1	153	1	154	9.54e-40	134
MS.gene26261.t1	MTR_3g065100	42.138	159	92	0	1	159	1	159	1.32e-38	131
MS.gene26261.t1	MTR_3g466830	43.506	154	87	0	4	157	3	156	1.07e-37	127
MS.gene26261.t1	MTR_3g466890	43.590	156	88	0	5	160	4	159	1.87e-36	124
MS.gene26261.t1	MTR_8g036130	41.026	156	89	2	1	153	1	156	2.07e-35	123
MS.gene26261.t1	MTR_2g016210	35.443	158	101	1	1	157	1	158	1.22e-34	121
MS.gene26261.t1	MTR_4g063790	38.125	160	92	2	1	156	1	157	1.39e-34	119
MS.gene26261.t1	MTR_4g032260	44.805	154	84	1	1	153	1	154	1.32e-33	116
MS.gene26261.t1	MTR_1g077320	39.103	156	94	1	1	156	1	155	1.01e-32	114
MS.gene26261.t1	MTR_1g077390	36.538	156	98	1	1	156	1	155	1.23e-32	114
MS.gene26261.t1	MTR_4g032290	44.156	154	85	1	1	153	1	154	3.05e-32	115
MS.gene26261.t1	MTR_1g077300	39.744	156	91	2	1	156	1	153	4.04e-32	112
MS.gene26261.t1	MTR_3g031100	35.714	154	98	1	1	153	1	154	3.62e-31	112
MS.gene26261.t1	MTR_5g047580	34.615	156	101	1	1	156	1	155	9.01e-31	109
MS.gene26261.t1	MTR_3g465410	40.152	132	72	2	29	156	1	129	1.58e-30	108
MS.gene26261.t1	MTR_7g011950	38.462	156	95	1	1	156	1	155	3.19e-30	107
MS.gene26261.t1	MTR_1g090697	35.032	157	101	1	1	157	1	156	8.21e-30	106
MS.gene26261.t1	MTR_4g028720	37.179	156	97	1	1	156	1	155	1.47e-29	106
MS.gene26261.t1	MTR_1g084950	37.179	156	97	1	1	156	1	155	9.58e-29	103
MS.gene26261.t1	MTR_2g035610	46.087	115	62	0	1	115	1	115	1.06e-28	102
MS.gene26261.t1	MTR_3g031240	33.117	154	102	1	1	153	1	154	7.57e-28	102
MS.gene26261.t1	MTR_1g090710	35.443	158	100	2	1	157	1	157	2.00e-26	98.2
MS.gene26261.t1	MTR_1g090783	32.468	154	103	1	4	157	3	155	2.20e-26	98.2
MS.gene26261.t1	MTR_3g466900	38.168	131	81	0	32	162	2	132	1.86e-25	94.7
MS.gene26261.t1	MTR_3g466930	38.760	129	79	0	32	160	2	130	3.63e-25	94.4
MS.gene26261.t1	MTR_2g035580	34.899	149	97	0	1	149	1	149	9.25e-25	94.0
MS.gene26261.t1	MTR_4g028800	54.795	73	31	1	1	73	1	71	1.70e-22	85.5
MS.gene26261.t1	MTR_3g093900	30.323	155	108	0	4	158	7	161	8.62e-21	88.2
MS.gene26261.t1	MTR_7g106510	32.484	157	104	2	4	159	7	162	1.86e-20	87.4
MS.gene26261.t1	MTR_4g019670	30.769	156	108	0	4	159	7	162	5.76e-20	85.9
MS.gene26261.t1	MTR_5g047560	30.769	143	82	2	1	143	1	126	2.27e-18	77.0
MS.gene26261.t1	MTR_6g018920	28.313	166	103	6	1	159	1	157	9.96e-15	70.5
MS.gene26261.t1	MTR_1g114730	32.773	119	72	4	1	112	1	118	1.58e-14	70.1
MS.gene26261.t1	MTR_6g005450	27.976	168	107	5	1	162	1	160	3.91e-12	63.2
MS.gene26261.t1	MTR_7g055790	33.962	106	52	1	4	109	62	149	1.32e-11	59.3
MS.gene26261.t1	MTR_6g005440	27.108	166	106	6	1	160	1	157	2.65e-11	60.8
MS.gene26261.t1	MTR_7g055800	34.177	79	37	1	31	109	18	81	4.14e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26261.t1	AT5G48670	39.610	154	91	2	1	153	1	153	3.15e-30	112
MS.gene26261.t1	AT3G05860	32.215	149	101	0	1	149	1	149	2.44e-27	102
MS.gene26261.t1	AT1G65300	35.664	143	92	0	4	146	3	145	6.75e-27	102
MS.gene26261.t1	AT3G05860	32.215	149	101	0	1	149	1	149	2.15e-26	100
MS.gene26261.t1	AT3G05860	32.215	149	101	0	1	149	1	149	3.32e-26	100
MS.gene26261.t1	AT1G65330	42.857	105	60	0	5	109	4	108	7.08e-26	99.8
MS.gene26261.t1	AT5G26650	44.340	106	59	0	4	109	2	107	5.91e-25	99.0
MS.gene26261.t1	AT1G31630	32.051	156	105	1	4	159	3	157	7.63e-25	98.2
MS.gene26261.t1	AT5G27960	43.396	106	60	0	4	109	2	107	1.38e-24	97.1
MS.gene26261.t1	AT5G26630	32.704	159	107	0	1	159	1	159	1.58e-24	95.1
MS.gene26261.t1	AT1G31640	33.553	152	92	4	5	152	4	150	6.65e-24	97.1
MS.gene26261.t1	AT5G27810	38.372	86	53	0	29	114	1	86	5.49e-20	80.5
MS.gene26261.t1	AT2G28700	31.333	150	101	2	1	149	1	149	7.07e-19	82.0
MS.gene26261.t1	AT2G40210	41.584	101	59	0	1	101	1	101	2.16e-18	80.9
MS.gene26261.t1	AT5G26580	38.532	109	53	1	1	109	1	95	2.71e-17	77.8
MS.gene26261.t1	AT5G06500	30.128	156	102	2	1	153	1	152	2.79e-17	76.3
MS.gene26261.t1	AT1G22590	28.289	152	100	2	1	149	1	146	2.32e-16	72.4
MS.gene26261.t1	AT2G15660	34.653	101	65	1	38	138	31	130	3.32e-12	62.8
MS.gene26261.t1	AT5G55690	35.455	110	59	3	1	103	1	105	1.12e-11	61.6
MS.gene26261.t1	AT5G55690	35.455	110	59	3	1	103	1	105	1.12e-11	61.6
MS.gene26261.t1	AT5G58890	37.273	110	62	2	1	104	1	109	2.04e-11	60.8

Find 41 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAACTTCTCATTCAACTTT+TGG	0.190821	5.4:-59075253	None:intergenic
ATGATCTTGAACCACAAATT+TGG	0.211992	5.4:+59075123	MS.gene26261:CDS
GATGAGAAAATGAGGGAAAT+TGG	0.282864	5.4:+59075398	MS.gene26261:CDS
TGCTCTCCTTGCTGTGTGAT+TGG	0.345198	5.4:-59075006	None:intergenic
TCTCATGGTTGAACATCTTT+CGG	0.376961	5.4:-59075211	None:intergenic
CTTCACTTTCTTTCCTGCCA+TGG	0.385906	5.4:-59074970	None:intergenic
ATTTGTGGTTCAAGATCATC+AGG	0.402521	5.4:-59075119	None:intergenic
GGAGAGCAACGTACAAGAAA+AGG	0.412101	5.4:+59075021	MS.gene26261:CDS
TGAATCGAACCAGCACACTA+TGG	0.431382	5.4:-59075163	None:intergenic
TGAACGGATGGCCAAATTTG+TGG	0.436771	5.4:-59075134	None:intergenic
TGGTATGAAGATGGTGCAAT+TGG	0.455791	5.4:+59075418	MS.gene26261:CDS
CAAATTTGGCCATCCGTTCA+AGG	0.457472	5.4:+59075137	MS.gene26261:CDS
CAACCATGAGAGTTACTTGA+AGG	0.466117	5.4:+59075223	MS.gene26261:CDS
TTTCTCAATACCACCATGGC+AGG	0.467282	5.4:+59074957	None:intergenic
TTACCTCACTATCTTGAGGC+AGG	0.500293	5.4:+59075320	MS.gene26261:CDS
TCTGAAGTGCTTAAATCCTC+AGG	0.504006	5.4:-59075350	None:intergenic
GAGAAGGATGAGAGGGCATA+GGG	0.528114	5.4:+59075440	MS.gene26261:CDS
GAGAGCAACGTACAAGAAAA+GGG	0.528840	5.4:+59075022	MS.gene26261:CDS
TTTCCTTCAAGTAACTCTCA+TGG	0.530880	5.4:-59075226	None:intergenic
ACATGCTTGAACTCCACATA+GGG	0.545908	5.4:-59075081	None:intergenic
TGCAATTGGAGAAGGATGAG+AGG	0.552324	5.4:+59075432	MS.gene26261:CDS
ATGAGAGTTACTTGAAGGAA+AGG	0.555751	5.4:+59075228	MS.gene26261:CDS
CTATGGACTCCTTGAACGGA+TGG	0.558436	5.4:-59075146	None:intergenic
AATGAAATCACAACCCTATG+TGG	0.562718	5.4:+59075068	MS.gene26261:CDS
CACACTATGGACTCCTTGAA+CGG	0.564303	5.4:-59075150	None:intergenic
TCAAGGAGTCCATAGTGTGC+TGG	0.565483	5.4:+59075154	MS.gene26261:CDS
ATGTGATTGATGAGAAAATG+AGG	0.567807	5.4:+59075390	MS.gene26261:CDS
GGAGAAGGATGAGAGGGCAT+AGG	0.568098	5.4:+59075439	MS.gene26261:CDS
GAAGATGGTGCAATTGGAGA+AGG	0.575400	5.4:+59075424	MS.gene26261:CDS
TACCTCACTATCTTGAGGCA+GGG	0.578476	5.4:+59075321	MS.gene26261:CDS
TGAAGGAGAATAGGATGATG+TGG	0.588946	5.4:+59075285	MS.gene26261:CDS
TCAATTACCTCACTATCTTG+AGG	0.600686	5.4:+59075316	MS.gene26261:CDS
GAGGGAAATTGGTATGAAGA+TGG	0.616379	5.4:+59075409	MS.gene26261:CDS
GCAATTGGAGAAGGATGAGA+GGG	0.619379	5.4:+59075433	MS.gene26261:CDS
CACATGCTTGAACTCCACAT+AGG	0.630810	5.4:-59075082	None:intergenic
TTCCCTGCCTCAAGATAGTG+AGG	0.632603	5.4:-59075323	None:intergenic
TGTGATTGATGAGAAAATGA+GGG	0.635288	5.4:+59075391	MS.gene26261:CDS
TCATAACCAATCACACAGCA+AGG	0.650603	5.4:+59075000	MS.gene26261:CDS
CTATGTGGAGTTCAAGCATG+TGG	0.662987	5.4:+59075083	MS.gene26261:CDS
TGAGGCAGGGAATATTCCTG+AGG	0.675847	5.4:+59075334	MS.gene26261:CDS
GGTTCGATTCATGCATACTG+CGG	0.703185	5.4:+59075175	MS.gene26261:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAAAAAATGAAGGAGAAT+AGG	+	chr5.4:59075276-59075295	MS.gene26261:CDS	25.0%
!	GAAGTTGAAGAAAAAAATGA+AGG	+	chr5.4:59075268-59075287	MS.gene26261:CDS	25.0%
	ATGATCTTGAACCACAAATT+TGG	+	chr5.4:59075123-59075142	MS.gene26261:CDS	30.0%
	ATGTGATTGATGAGAAAATG+AGG	+	chr5.4:59075390-59075409	MS.gene26261:CDS	30.0%
	TGTGATTGATGAGAAAATGA+GGG	+	chr5.4:59075391-59075410	MS.gene26261:CDS	30.0%
!	TCAACTTCTCATTCAACTTT+TGG	-	chr5.4:59075256-59075275	None:intergenic	30.0%
	AATGAAATCACAACCCTATG+TGG	+	chr5.4:59075068-59075087	MS.gene26261:CDS	35.0%
	ATGAGAGTTACTTGAAGGAA+AGG	+	chr5.4:59075228-59075247	MS.gene26261:CDS	35.0%
	ATTTGTGGTTCAAGATCATC+AGG	-	chr5.4:59075122-59075141	None:intergenic	35.0%
	GATGAGAAAATGAGGGAAAT+TGG	+	chr5.4:59075398-59075417	MS.gene26261:CDS	35.0%
	TCAATTACCTCACTATCTTG+AGG	+	chr5.4:59075316-59075335	MS.gene26261:CDS	35.0%
	TCTCATGGTTGAACATCTTT+CGG	-	chr5.4:59075214-59075233	None:intergenic	35.0%
	TTTCCTTCAAGTAACTCTCA+TGG	-	chr5.4:59075229-59075248	None:intergenic	35.0%
	ACATGCTTGAACTCCACATA+GGG	-	chr5.4:59075084-59075103	None:intergenic	40.0%
	CAACCATGAGAGTTACTTGA+AGG	+	chr5.4:59075223-59075242	MS.gene26261:CDS	40.0%
	GAGAGCAACGTACAAGAAAA+GGG	+	chr5.4:59075022-59075041	MS.gene26261:CDS	40.0%
	GAGGGAAATTGGTATGAAGA+TGG	+	chr5.4:59075409-59075428	MS.gene26261:CDS	40.0%
	TCATAACCAATCACACAGCA+AGG	+	chr5.4:59075000-59075019	MS.gene26261:CDS	40.0%
	TGAAGGAGAATAGGATGATG+TGG	+	chr5.4:59075285-59075304	MS.gene26261:CDS	40.0%
	TGGTATGAAGATGGTGCAAT+TGG	+	chr5.4:59075418-59075437	MS.gene26261:CDS	40.0%
!	TCTGAAGTGCTTAAATCCTC+AGG	-	chr5.4:59075353-59075372	None:intergenic	40.0%
	CAAATTTGGCCATCCGTTCA+AGG	+	chr5.4:59075137-59075156	MS.gene26261:CDS	45.0%
	CACACTATGGACTCCTTGAA+CGG	-	chr5.4:59075153-59075172	None:intergenic	45.0%
	CACATGCTTGAACTCCACAT+AGG	-	chr5.4:59075085-59075104	None:intergenic	45.0%
	CTATGTGGAGTTCAAGCATG+TGG	+	chr5.4:59075083-59075102	MS.gene26261:CDS	45.0%
	GAAGATGGTGCAATTGGAGA+AGG	+	chr5.4:59075424-59075443	MS.gene26261:CDS	45.0%
	GCAATTGGAGAAGGATGAGA+GGG	+	chr5.4:59075433-59075452	MS.gene26261:CDS	45.0%
	GGAGAGCAACGTACAAGAAA+AGG	+	chr5.4:59075021-59075040	MS.gene26261:CDS	45.0%
	GGTTCGATTCATGCATACTG+CGG	+	chr5.4:59075175-59075194	MS.gene26261:CDS	45.0%
	TACCTCACTATCTTGAGGCA+GGG	+	chr5.4:59075321-59075340	MS.gene26261:CDS	45.0%
	TGAACGGATGGCCAAATTTG+TGG	-	chr5.4:59075137-59075156	None:intergenic	45.0%
	TGAATCGAACCAGCACACTA+TGG	-	chr5.4:59075166-59075185	None:intergenic	45.0%
	TGCAATTGGAGAAGGATGAG+AGG	+	chr5.4:59075432-59075451	MS.gene26261:CDS	45.0%
	TTACCTCACTATCTTGAGGC+AGG	+	chr5.4:59075320-59075339	MS.gene26261:CDS	45.0%
	CTATGGACTCCTTGAACGGA+TGG	-	chr5.4:59075149-59075168	None:intergenic	50.0%
	TCAAGGAGTCCATAGTGTGC+TGG	+	chr5.4:59075154-59075173	MS.gene26261:CDS	50.0%
	TGAGGCAGGGAATATTCCTG+AGG	+	chr5.4:59075334-59075353	MS.gene26261:CDS	50.0%
	TTCCCTGCCTCAAGATAGTG+AGG	-	chr5.4:59075326-59075345	None:intergenic	50.0%
!	TGCTCTCCTTGCTGTGTGAT+TGG	-	chr5.4:59075009-59075028	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	59074972	59075460	59074972	ID=MS.gene26261
chr5.4	mRNA	59074972	59075460	59074972	ID=MS.gene26261.t1;Parent=MS.gene26261
chr5.4	exon	59074972	59075460	59074972	ID=MS.gene26261.t1.exon1;Parent=MS.gene26261.t1
chr5.4	CDS	59074972	59075460	59074972	ID=cds.MS.gene26261.t1;Parent=MS.gene26261.t1

Gene Sequence

>MS.gene26261

ATGGCAGGAAAGAAAGTGAAGCTTGCATTCATAACCAATCACACAGCAAGGAGAGCAACGTACAAGAAAAGGGTACAAAGTTTGATGAAGAAGCTCAATGAAATCACAACCCTATGTGGAGTTCAAGCATGTGGTATAGTTTTCAAGCCTGATGATCTTGAACCACAAATTTGGCCATCCGTTCAAGGAGTCCATAGTGTGCTGGTTCGATTCATGCATACTGCGGACTTCGATCGAGACCGAAAGATGTTCAACCATGAGAGTTACTTGAAGGAAAGGATCCAAAAGTTGAATGAGAAGTTGAAGAAAAAAATGAAGGAGAATAGGATGATGTGGATGAGTCTTCAATTACCTCACTATCTTGAGGCAGGGAATATTCCTGAGGATTTAAGCACTTCAGATATGAATGATCTCACATATGTGATTGATGAGAAAATGAGGGAAATTGGTATGAAGATGGTGCAATTGGAGAAGGATGAGAGGGCATAG

Protein sequence

>MS.gene26261.t1

MAGKKVKLAFITNHTARRATYKKRVQSLMKKLNEITTLCGVQACGIVFKPDDLEPQIWPSVQGVHSVLVRFMHTADFDRDRKMFNHESYLKERIQKLNEKLKKKMKENRMMWMSLQLPHYLEAGNIPEDLSTSDMNDLTYVIDEKMREIGMKMVQLEKDERA