Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26275.t1 | XP_003616018.1 | 97.7 | 349 | 8 | 0 | 1 | 349 | 1 | 349 | 7.10E-183 | 649.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26275.t1 | Q9LVL4 | 35.5 | 349 | 159 | 13 | 1 | 346 | 5 | 290 | 8.3e-31 | 136.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26275.t1 | G7K0S1 | 97.7 | 349 | 8 | 0 | 1 | 349 | 1 | 349 | 5.1e-183 | 649.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene26275.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26275.t1 | MTR_5g075210 | 97.708 | 349 | 8 | 0 | 1 | 349 | 1 | 349 | 0.0 | 699 |
| MS.gene26275.t1 | MTR_7g072630 | 39.793 | 387 | 150 | 16 | 1 | 345 | 1 | 346 | 1.21e-64 | 209 |
| MS.gene26275.t1 | MTR_7g072875 | 41.528 | 301 | 129 | 12 | 65 | 345 | 11 | 284 | 1.45e-51 | 173 |
| MS.gene26275.t1 | MTR_4g028930 | 45.930 | 172 | 72 | 4 | 181 | 346 | 224 | 380 | 7.58e-35 | 131 |
| MS.gene26275.t1 | MTR_8g087530 | 41.429 | 140 | 64 | 4 | 224 | 346 | 186 | 324 | 7.19e-24 | 100 |
| MS.gene26275.t1 | MTR_2g018030 | 33.333 | 171 | 84 | 5 | 197 | 346 | 182 | 343 | 3.88e-22 | 96.3 |
| MS.gene26275.t1 | MTR_0907s0020 | 51.613 | 62 | 30 | 0 | 280 | 341 | 102 | 163 | 3.48e-16 | 76.3 |
| MS.gene26275.t1 | MTR_8g103520 | 56.452 | 62 | 27 | 0 | 280 | 341 | 224 | 285 | 6.70e-16 | 77.4 |
| MS.gene26275.t1 | MTR_4g052040 | 57.377 | 61 | 26 | 0 | 283 | 343 | 102 | 162 | 7.07e-15 | 72.4 |
| MS.gene26275.t1 | MTR_7g105790 | 48.485 | 66 | 34 | 0 | 283 | 348 | 113 | 178 | 1.31e-14 | 72.0 |
| MS.gene26275.t1 | MTR_3g111780 | 43.000 | 100 | 57 | 0 | 245 | 344 | 163 | 262 | 2.24e-13 | 70.1 |
| MS.gene26275.t1 | MTR_4g132740 | 35.165 | 91 | 56 | 1 | 257 | 344 | 55 | 145 | 7.34e-13 | 65.9 |
| MS.gene26275.t1 | MTR_5g079710 | 43.284 | 67 | 38 | 0 | 271 | 337 | 82 | 148 | 8.40e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26275.t1 | AT5G58360 | 36.676 | 349 | 155 | 12 | 1 | 346 | 5 | 290 | 3.19e-46 | 159 |
| MS.gene26275.t1 | AT1G06920 | 34.615 | 364 | 161 | 14 | 1 | 344 | 1 | 307 | 1.97e-44 | 155 |
| MS.gene26275.t1 | AT2G30400 | 35.754 | 358 | 182 | 13 | 1 | 348 | 1 | 320 | 5.88e-41 | 146 |
| MS.gene26275.t1 | AT5G01840 | 50.467 | 107 | 45 | 2 | 239 | 344 | 163 | 262 | 1.82e-24 | 101 |
| MS.gene26275.t1 | AT2G18500 | 53.623 | 69 | 32 | 0 | 276 | 344 | 222 | 290 | 1.55e-17 | 82.4 |
| MS.gene26275.t1 | AT4G18830 | 47.126 | 87 | 42 | 2 | 261 | 343 | 259 | 345 | 2.12e-17 | 82.4 |
| MS.gene26275.t1 | AT5G19650 | 51.515 | 66 | 32 | 0 | 279 | 344 | 153 | 218 | 1.74e-15 | 75.1 |
| MS.gene26275.t1 | AT3G52525 | 52.459 | 61 | 29 | 0 | 283 | 343 | 69 | 129 | 1.02e-14 | 71.2 |
| MS.gene26275.t1 | AT2G36026 | 50.000 | 56 | 28 | 0 | 286 | 341 | 92 | 147 | 2.04e-13 | 68.6 |
| MS.gene26275.t1 | AT4G04025 | 47.619 | 63 | 33 | 0 | 278 | 340 | 111 | 173 | 2.18e-11 | 62.4 |
Find 39 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTCCTTAAAATCACTTAT+TGG | 0.188683 | 5.4:+59213222 | None:intergenic |
| CTTCTTGGTGAATCATTAAA+TGG | 0.215429 | 5.4:+59213849 | None:intergenic |
| TTCACTTGCTTGGTCAATAT+TGG | 0.218504 | 5.4:+59213522 | None:intergenic |
| AGCTTTATACCTTGAGAATT+AGG | 0.222952 | 5.4:+59213363 | None:intergenic |
| AAATGGAAAATTCAAATCTT+TGG | 0.240418 | 5.4:+59213866 | None:intergenic |
| TTCAAATCTTTGGTTTGAAT+TGG | 0.305617 | 5.4:+59213876 | None:intergenic |
| TTCTTCAAATTTCACTTGCT+TGG | 0.306007 | 5.4:+59213512 | None:intergenic |
| TATAAGGGAATCAAAGGATT+TGG | 0.325148 | 5.4:-59213172 | MS.gene26275:CDS |
| TGATTGTGGAGAATAATATA+AGG | 0.326543 | 5.4:-59213188 | MS.gene26275:CDS |
| ATTTCTCAAATGAACCAAAA+AGG | 0.337952 | 5.4:-59213920 | MS.gene26275:CDS |
| AATGATGCAGGGTTTGTAGA+TGG | 0.356794 | 5.4:-59213273 | MS.gene26275:CDS |
| TGGTTGAGAAGGCTTGTAAA+TGG | 0.361373 | 5.4:+59213797 | None:intergenic |
| TTCAGTTGAACTTCGCGATT+CGG | 0.365379 | 5.4:+59213473 | None:intergenic |
| CAACTACAGTTAAGCAAATC+AGG | 0.377071 | 5.4:+59213657 | None:intergenic |
| AAGATCTTTACATTCTAAGT+TGG | 0.393025 | 5.4:+59213058 | None:intergenic |
| AGCTTGTAAAACCAAGCATT+TGG | 0.394500 | 5.4:+59214056 | None:intergenic |
| AGATCTTTACATTCTAAGTT+GGG | 0.428724 | 5.4:+59213059 | None:intergenic |
| AGTATTTCAAAGAATGATGC+AGG | 0.439556 | 5.4:-59213285 | MS.gene26275:CDS |
| TCTCCTTCTTTGAGTAACTT+TGG | 0.446790 | 5.4:+59213416 | None:intergenic |
| TGTGATTGAACTTGATCAAA+TGG | 0.456333 | 5.4:+59213777 | None:intergenic |
| CAGATATGATACCAAATGCT+TGG | 0.463165 | 5.4:-59214067 | MS.gene26275:CDS |
| ACTTGCTTGGTCAATATTGG+AGG | 0.476021 | 5.4:+59213525 | None:intergenic |
| TATCTCTTATGGGAAACAAC+AGG | 0.478460 | 5.4:-59214100 | None:intergenic |
| CTACCAAAGTTACTCAAAGA+AGG | 0.488250 | 5.4:-59213419 | MS.gene26275:CDS |
| AAATCATCAAGGAAGAGAAA+TGG | 0.497520 | 5.4:-59214020 | MS.gene26275:CDS |
| AAGGGAATCAAAGGATTTGG+AGG | 0.497577 | 5.4:-59213169 | MS.gene26275:CDS |
| TGAGACCTTTGAGATGTTGT+TGG | 0.501197 | 5.4:+59213960 | None:intergenic |
| GATTGTGGAGAATAATATAA+GGG | 0.512687 | 5.4:-59213187 | MS.gene26275:CDS |
| ACTTGATCAAATGGTTGAGA+AGG | 0.519734 | 5.4:+59213786 | None:intergenic |
| CATTTAATGATTCACCAAGA+AGG | 0.535424 | 5.4:-59213848 | MS.gene26275:CDS |
| GAATAATATAAGGGAATCAA+AGG | 0.538128 | 5.4:-59213178 | MS.gene26275:CDS |
| AAAACTTCTCCTAATTCTCA+AGG | 0.546162 | 5.4:-59213372 | MS.gene26275:CDS |
| AAGATATGAGCAAATCATCA+AGG | 0.573800 | 5.4:-59214031 | MS.gene26275:CDS |
| TGAGCAAATTTGGTATGACA+TGG | 0.588029 | 5.4:-59213082 | MS.gene26275:CDS |
| ACTTCGCGATTCGGTCACCT+TGG | 0.590520 | 5.4:+59213482 | None:intergenic |
| ATCAATGGTGGAGATGATTG+TGG | 0.597753 | 5.4:-59213202 | MS.gene26275:CDS |
| CTTCACCAACAACATCTCAA+AGG | 0.609838 | 5.4:-59213965 | MS.gene26275:CDS |
| ATTTGAAGAAAAGATTGCCA+AGG | 0.612353 | 5.4:-59213499 | MS.gene26275:CDS |
| GTATTTCAAAGAATGATGCA+GGG | 0.644805 | 5.4:-59213284 | MS.gene26275:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGGAAAATTCAAATCTT+TGG | + | chr5.4:59213294-59213313 | None:intergenic | 20.0% |
| !!! | ATTTTTTTGCTAACAAGTTT+TGG | + | chr5.4:59213833-59213852 | None:intergenic | 20.0% |
| ! | ATTTCTCAAATGAACCAAAA+AGG | - | chr5.4:59213237-59213256 | MS.gene26275:CDS | 25.0% |
| ! | GAATAATATAAGGGAATCAA+AGG | - | chr5.4:59213979-59213998 | MS.gene26275:CDS | 25.0% |
| ! | GATTCCTTAAAATCACTTAT+TGG | + | chr5.4:59213938-59213957 | None:intergenic | 25.0% |
| ! | GATTGTGGAGAATAATATAA+GGG | - | chr5.4:59213970-59213989 | MS.gene26275:CDS | 25.0% |
| ! | TGATTGTGGAGAATAATATA+AGG | - | chr5.4:59213969-59213988 | MS.gene26275:CDS | 25.0% |
| !! | AATAACTTACCATTCCTTTT+TGG | + | chr5.4:59213254-59213273 | None:intergenic | 25.0% |
| !! | ATGAACAATGAATCTTTTCA+AGG | - | chr5.4:59213560-59213579 | MS.gene26275:CDS | 25.0% |
| !! | TGATCCAATAAGTGATTTTA+AGG | - | chr5.4:59213931-59213950 | MS.gene26275:CDS | 25.0% |
| !! | TTAAAGCTTTTGAGCAAATT+TGG | - | chr5.4:59214065-59214084 | MS.gene26275:CDS | 25.0% |
| !!! | AAGTGATTTTAAGGAATCAA+TGG | - | chr5.4:59213940-59213959 | MS.gene26275:CDS | 25.0% |
| !!! | TTCAAATCTTTGGTTTGAAT+TGG | + | chr5.4:59213284-59213303 | None:intergenic | 25.0% |
| AAAACTTCTCCTAATTCTCA+AGG | - | chr5.4:59213785-59213804 | MS.gene26275:CDS | 30.0% | |
| AAATCATCAAGGAAGAGAAA+TGG | - | chr5.4:59213137-59213156 | MS.gene26275:CDS | 30.0% | |
| AAGAAGGTCTTCAAAAAGAA+GGG | - | chr5.4:59213325-59213344 | MS.gene26275:CDS | 30.0% | |
| AAGATATGAGCAAATCATCA+AGG | - | chr5.4:59213126-59213145 | MS.gene26275:CDS | 30.0% | |
| AGTATTTCAAAGAATGATGC+AGG | - | chr5.4:59213872-59213891 | MS.gene26275:CDS | 30.0% | |
| ATTTGAAGAAAAGATTGCCA+AGG | - | chr5.4:59213658-59213677 | MS.gene26275:CDS | 30.0% | |
| CATTTAATGATTCACCAAGA+AGG | - | chr5.4:59213309-59213328 | MS.gene26275:CDS | 30.0% | |
| CTTCTTGGTGAATCATTAAA+TGG | + | chr5.4:59213311-59213330 | None:intergenic | 30.0% | |
| GTATTTCAAAGAATGATGCA+GGG | - | chr5.4:59213873-59213892 | MS.gene26275:CDS | 30.0% | |
| TATAAGGGAATCAAAGGATT+TGG | - | chr5.4:59213985-59214004 | MS.gene26275:CDS | 30.0% | |
| TCAAATGAACCAAAAAGGAA+TGG | - | chr5.4:59213242-59213261 | MS.gene26275:CDS | 30.0% | |
| TGTGATTGAACTTGATCAAA+TGG | + | chr5.4:59213383-59213402 | None:intergenic | 30.0% | |
| TTCTTCAAATTTCACTTGCT+TGG | + | chr5.4:59213648-59213667 | None:intergenic | 30.0% | |
| ! | AGAATTAGGAGAAGTTTTTC+TGG | + | chr5.4:59213783-59213802 | None:intergenic | 30.0% |
| ! | AGCTTTATACCTTGAGAATT+AGG | + | chr5.4:59213797-59213816 | None:intergenic | 30.0% |
| ! | GGCAATTCTAAAAACTTAGA+TGG | - | chr5.4:59213581-59213600 | MS.gene26275:CDS | 30.0% |
| ! | TTTGATGCATTTTCACAACT+TGG | - | chr5.4:59213605-59213624 | MS.gene26275:CDS | 30.0% |
| !! | TGATTTTAAGGAATCAATGG+TGG | - | chr5.4:59213943-59213962 | MS.gene26275:CDS | 30.0% |
| ACTTGATCAAATGGTTGAGA+AGG | + | chr5.4:59213374-59213393 | None:intergenic | 35.0% | |
| AGCTTGTAAAACCAAGCATT+TGG | + | chr5.4:59213104-59213123 | None:intergenic | 35.0% | |
| CAACTACAGTTAAGCAAATC+AGG | + | chr5.4:59213503-59213522 | None:intergenic | 35.0% | |
| CAAGAAGGTCTTCAAAAAGA+AGG | - | chr5.4:59213324-59213343 | MS.gene26275:CDS | 35.0% | |
| CAGATATGATACCAAATGCT+TGG | - | chr5.4:59213090-59213109 | MS.gene26275:CDS | 35.0% | |
| CTACCAAAGTTACTCAAAGA+AGG | - | chr5.4:59213738-59213757 | MS.gene26275:CDS | 35.0% | |
| TCTCCTTCTTTGAGTAACTT+TGG | + | chr5.4:59213744-59213763 | None:intergenic | 35.0% | |
| TGAGCAAATTTGGTATGACA+TGG | - | chr5.4:59214075-59214094 | MS.gene26275:CDS | 35.0% | |
| TTCACTTGCTTGGTCAATAT+TGG | + | chr5.4:59213638-59213657 | None:intergenic | 35.0% | |
| !! | CTTCTTTTTGAAGACCTTCT+TGG | + | chr5.4:59213326-59213345 | None:intergenic | 35.0% |
| AAGGGAATCAAAGGATTTGG+AGG | - | chr5.4:59213988-59214007 | MS.gene26275:CDS | 40.0% | |
| AATGATGCAGGGTTTGTAGA+TGG | - | chr5.4:59213884-59213903 | MS.gene26275:CDS | 40.0% | |
| ACTTGCTTGGTCAATATTGG+AGG | + | chr5.4:59213635-59213654 | None:intergenic | 40.0% | |
| CTTCACCAACAACATCTCAA+AGG | - | chr5.4:59213192-59213211 | MS.gene26275:CDS | 40.0% | |
| TGGTTGAGAAGGCTTGTAAA+TGG | + | chr5.4:59213363-59213382 | None:intergenic | 40.0% | |
| TTCAGTTGAACTTCGCGATT+CGG | + | chr5.4:59213687-59213706 | None:intergenic | 40.0% | |
| ! | ATCAATGGTGGAGATGATTG+TGG | - | chr5.4:59213955-59213974 | MS.gene26275:CDS | 40.0% |
| !! | TGAGACCTTTGAGATGTTGT+TGG | + | chr5.4:59213200-59213219 | None:intergenic | 40.0% |
| !! | ACTTCGCGATTCGGTCACCT+TGG | + | chr5.4:59213678-59213697 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 59213065 | 59214114 | 59213065 | ID=MS.gene26275 |
| chr5.4 | mRNA | 59213065 | 59214114 | 59213065 | ID=MS.gene26275.t1;Parent=MS.gene26275 |
| chr5.4 | exon | 59213065 | 59214114 | 59213065 | ID=MS.gene26275.t1.exon1;Parent=MS.gene26275.t1 |
| chr5.4 | CDS | 59213065 | 59214114 | 59213065 | ID=cds.MS.gene26275.t1;Parent=MS.gene26275.t1 |
| Gene Sequence |
| Protein sequence |
