Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26319.t1 | PNX89523.1 | 39.6 | 338 | 188 | 5 | 17 | 352 | 11 | 334 | 1.50E-10 | 77.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26319.t1 | A0A2K3MFE8 | 39.6 | 338 | 188 | 5 | 17 | 352 | 11 | 334 | 1.1e-10 | 77.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene26319.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 161 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTTAAAAAAGAACAC+AGG | - | 37913:6908-6927 | MS.gene26319:CDS | 15.0% |
| !!! | AAATGATAACAATTTAAAAC+AGG | + | 37913:7850-7869 | None:intergenic | 15.0% |
| !!! | AATTACTGTTATATAGTTTT+TGG | - | 37913:7809-7828 | MS.gene26319:intron | 15.0% |
| ! | AAATCAAAGAAATTATGTGG+AGG | + | 37913:7556-7575 | None:intergenic | 25.0% |
| ! | AGTACACAAAATCTTAGATA+CGG | + | 37913:6568-6587 | None:intergenic | 25.0% |
| ! | GCAAAATCAAAGAAATTATG+TGG | + | 37913:7559-7578 | None:intergenic | 25.0% |
| !!! | CATTTGTTGTTACATGTTTA+AGG | - | 37913:6455-6474 | MS.gene26319:CDS | 25.0% |
| AAATATATTGCAGAGTGACA+AGG | + | 37913:6651-6670 | None:intergenic | 30.0% | |
| AGTACACATTATCGTAGATA+CGG | + | 37913:6755-6774 | None:intergenic | 30.0% | |
| ATTACAGAGTGACAAAAAAC+TGG | + | 37913:6799-6818 | None:intergenic | 30.0% | |
| TATAATTCATACTCCTCCAA+GGG | + | 37913:7328-7347 | None:intergenic | 30.0% | |
| TTACAGAGTGACAAAAAACT+GGG | + | 37913:6798-6817 | None:intergenic | 30.0% | |
| TTGTTACTAAACTTTGAGTG+TGG | - | 37913:6352-6371 | MS.gene26319:CDS | 30.0% | |
| AAAATCTACCGGATACCAAA+AGG | + | 37913:6426-6445 | None:intergenic | 35.0% | |
| AAATCTACCGGATACCAAAA+GGG | + | 37913:6425-6444 | None:intergenic | 35.0% | |
| AAGTGGTTGTTGAAGTATGA+TGG | + | 37913:7028-7047 | None:intergenic | 35.0% | |
| ACCCATATGTTATGTATGCT+AGG | - | 37913:8098-8117 | MS.gene26319:CDS | 35.0% | |
| ACTCCTTAGCCTATAGAAAA+AGG | + | 37913:7137-7156 | None:intergenic | 35.0% | |
| ATACTGCGTCTAAGTTACAA+CGG | - | 37913:7190-7209 | MS.gene26319:CDS | 35.0% | |
| ATCTTCATCGTTGATATCCA+TGG | + | 37913:7587-7606 | None:intergenic | 35.0% | |
| ATTACAGAGTGACAACAAAC+AGG | + | 37913:6612-6631 | None:intergenic | 35.0% | |
| CCCATATGTTATGTATGCTA+GGG | - | 37913:8099-8118 | MS.gene26319:CDS | 35.0% | |
| CCCTAGCATACATAACATAT+GGG | + | 37913:8102-8121 | None:intergenic | 35.0% | |
| GGTAACAATGGTGAAAACAT+GGG | - | 37913:7667-7686 | MS.gene26319:intron | 35.0% | |
| GTATAATTCATACTCCTCCA+AGG | + | 37913:7329-7348 | None:intergenic | 35.0% | |
| TCAAAGAAATTATGTGGAGG+CGG | + | 37913:7553-7572 | None:intergenic | 35.0% | |
| TCATACTTCAACAACCACTT+TGG | - | 37913:7028-7047 | MS.gene26319:CDS | 35.0% | |
| TCCCTAGCATACATAACATA+TGG | + | 37913:8103-8122 | None:intergenic | 35.0% | |
| TTCTTGTGTGTGCATAATAG+TGG | + | 37913:6544-6563 | None:intergenic | 35.0% | |
| TTGATTGGCATCATGAACTT+CGG | + | 37913:7500-7519 | None:intergenic | 35.0% | |
| TTTACAGAGTGACATCAAAC+TGG | + | 37913:6837-6856 | None:intergenic | 35.0% | |
| ! | GAAGTTGTCACTTGTAATGT+AGG | - | 37913:6988-7007 | MS.gene26319:CDS | 35.0% |
| ! | TTATTTTCCTGTCTCCCTTT+TGG | - | 37913:6408-6427 | MS.gene26319:CDS | 35.0% |
| AACATCAATGCCAAGATCGA+TGG | + | 37913:7392-7411 | None:intergenic | 40.0% | |
| AACCAACAACAAGGCAAACA+TGG | + | 37913:6677-6696 | None:intergenic | 40.0% | |
| AACGATGAAGATGTGGAAGA+TGG | - | 37913:7595-7614 | MS.gene26319:intron | 40.0% | |
| ACTCGTGCAACAAATCTTCT+TGG | + | 37913:7887-7906 | None:intergenic | 40.0% | |
| ATCCTACTTACAGATGCCAT+GGG | - | 37913:7932-7951 | MS.gene26319:intron | 40.0% | |
| ATGTTTGCCTTCTTGTTGGT+TGG | - | 37913:6487-6506 | MS.gene26319:CDS | 40.0% | |
| GACTATGTTTGCCTTCTTGT+TGG | - | 37913:6483-6502 | MS.gene26319:CDS | 40.0% | |
| GCAGCATCAAAGAAGTTATG+CGG | + | 37913:7634-7653 | None:intergenic | 40.0% | |
| GGATATCAACGATGAAGATG+TGG | - | 37913:7588-7607 | MS.gene26319:intron | 40.0% | |
| GGGTAACAATGGTGAAAACA+TGG | - | 37913:7666-7685 | MS.gene26319:intron | 40.0% | |
| GTCACTTGTAATGTAGGATG+AGG | - | 37913:6994-7013 | MS.gene26319:CDS | 40.0% | |
| GTGCAACAAATCTTCTTGGT+AGG | + | 37913:7883-7902 | None:intergenic | 40.0% | |
| GTGGGAATGTGGCTAATAAT+GGG | - | 37913:7695-7714 | MS.gene26319:intron | 40.0% | |
| TAAGACACACTTACCTTGTC+TGG | + | 37913:6318-6337 | None:intergenic | 40.0% | |
| TCATCATCCAACCAACAACA+AGG | + | 37913:6686-6705 | None:intergenic | 40.0% | |
| TCATCATCCAACCAACAAGA+AGG | + | 37913:6497-6516 | None:intergenic | 40.0% | |
| TGAAGCCATGCAAAATCTAC+CGG | + | 37913:6437-6456 | None:intergenic | 40.0% | |
| TGATTGGCATCATGAACTTC+GGG | + | 37913:7499-7518 | None:intergenic | 40.0% | |
| TGTCAGACTTGCAGAATACA+TGG | - | 37913:7735-7754 | MS.gene26319:intron | 40.0% | |
| TTATGTATGCTAGGGAAGCT+CGG | - | 37913:8107-8126 | MS.gene26319:CDS | 40.0% | |
| ! | GGACCTTTTTCTATAGGCTA+AGG | - | 37913:7131-7150 | MS.gene26319:CDS | 40.0% |
| ! | GGTGTTTTCATAGGAAGTGA+GGG | - | 37913:7103-7122 | MS.gene26319:CDS | 40.0% |
| ! | GTGTTTTCATAGGAAGTGAG+GGG | - | 37913:7104-7123 | MS.gene26319:CDS | 40.0% |
| ! | GTTTTCACCATTGTTACCCA+TGG | + | 37913:7665-7684 | None:intergenic | 40.0% |
| ! | TGGTGTTTTCATAGGAAGTG+AGG | - | 37913:7102-7121 | MS.gene26319:CDS | 40.0% |
| !! | AATTATGTGGAGGCGGATTT+TGG | + | 37913:7546-7565 | None:intergenic | 40.0% |
| !! | AGAGATTTGTTACGGAGAAC+CGG | - | 37913:8032-8051 | MS.gene26319:intron | 40.0% |
| !! | AGATTTGTTGCACGAGTGTT+TGG | - | 37913:7890-7909 | MS.gene26319:intron | 40.0% |
| !! | ATGTTTGCCTTGTTGTTGGT+TGG | - | 37913:6676-6695 | MS.gene26319:CDS | 40.0% |
| !! | ATTATGTGGAGGCGGATTTT+GGG | + | 37913:7545-7564 | None:intergenic | 40.0% |
| !! | GATTTGTTGCACGAGTGTTT+GGG | - | 37913:7891-7910 | MS.gene26319:intron | 40.0% |
| !!! | TCGAGGTTTGGTGTTTTCAT+AGG | - | 37913:7094-7113 | MS.gene26319:CDS | 40.0% |
| AATGGTGAAAACATGGGTGC+AGG | - | 37913:7673-7692 | MS.gene26319:intron | 45.0% | |
| AGACGCAGTATGAGACCTAA+CGG | + | 37913:7181-7200 | None:intergenic | 45.0% | |
| ATCGTCGTCGTTGTCTTCAT+CGG | + | 37913:7365-7384 | None:intergenic | 45.0% | |
| ATGAACTACGACACAGAGTC+TGG | - | 37913:7053-7072 | MS.gene26319:CDS | 45.0% | |
| CACAACAATCGATCCAGACA+AGG | - | 37913:6302-6321 | MS.gene26319:CDS | 45.0% | |
| CATCCTACTTACAGATGCCA+TGG | - | 37913:7931-7950 | MS.gene26319:intron | 45.0% | |
| CATGGCATCTGTAAGTAGGA+TGG | + | 37913:7933-7952 | None:intergenic | 45.0% | |
| CATGGTGAAGACTAGCAATC+TGG | - | 37913:7753-7772 | MS.gene26319:intron | 45.0% | |
| CGTCTAAGTTACAACGGACA+GGG | - | 37913:7196-7215 | MS.gene26319:CDS | 45.0% | |
| CTATAGGCTAAGGAGTTTGC+TGG | - | 37913:7141-7160 | MS.gene26319:CDS | 45.0% | |
| GAACTTCCTGAATGTAAGCG+CGG | + | 37913:6280-6299 | None:intergenic | 45.0% | |
| GACGATGATTCCATCGATCT+TGG | - | 37913:7379-7398 | MS.gene26319:CDS | 45.0% | |
| GCATCAAAGAAGTTATGCGG+AGG | + | 37913:7631-7650 | None:intergenic | 45.0% | |
| GCATCTGTAAGTAGGATGGT+GGG | + | 37913:7929-7948 | None:intergenic | 45.0% | |
| GGCATTGTGTGGTTGTTGAT+TGG | + | 37913:7515-7534 | None:intergenic | 45.0% | |
| GGTGGGAATGTGGCTAATAA+TGG | - | 37913:7694-7713 | MS.gene26319:intron | 45.0% | |
| GTCGTAGTTCATCACCAAAG+TGG | + | 37913:7045-7064 | None:intergenic | 45.0% | |
| GTCTAAGTTACAACGGACAG+GGG | - | 37913:7197-7216 | MS.gene26319:CDS | 45.0% | |
| GTCTGGTTAGAAGACACAAG+AGG | - | 37913:7070-7089 | MS.gene26319:CDS | 45.0% | |
| GTGAAGACTAGCAATCTGGT+AGG | - | 37913:7757-7776 | MS.gene26319:intron | 45.0% | |
| TAGAAGACACAAGAGGTTCG+AGG | - | 37913:7077-7096 | MS.gene26319:CDS | 45.0% | |
| ! | GACCATGTTTGCCTTGTTGT+TGG | - | 37913:6672-6691 | MS.gene26319:CDS | 45.0% |
| ! | GGTATCCGGTAGATTTTGCA+TGG | - | 37913:6429-6448 | MS.gene26319:CDS | 45.0% |
| ! | TGTGGCTAATAATGGGAGAG+TGG | - | 37913:7702-7721 | MS.gene26319:intron | 45.0% |
| !! | CGGGTGGAAGAGATTTGTTA+CGG | - | 37913:8024-8043 | MS.gene26319:intron | 45.0% |
| !! | CTTTGATTTTGCTGCTGCCA+TGG | - | 37913:7567-7586 | MS.gene26319:intron | 45.0% |
| !! | TGGCAGTTGGGAAAAGTGTT+AGG | - | 37913:8056-8075 | MS.gene26319:intron | 45.0% |
| ACATCAACATCCGCTGGATC+CGG | + | 37913:7454-7473 | None:intergenic | 50.0% | |
| ACTCGACATGACCGAGCTAA+CGG | - | 37913:7243-7262 | MS.gene26319:CDS | 50.0% | |
| ACTTTCCGTGCAGGATCAAG+TGG | - | 37913:7960-7979 | MS.gene26319:intron | 50.0% | |
| AGCCCATGGCATCTGTAAGT+AGG | + | 37913:7937-7956 | None:intergenic | 50.0% | |
| AGGATCAAGTGGAAGGACAG+AGG | - | 37913:7971-7990 | MS.gene26319:intron | 50.0% | |
| ATCTCGGCAGCAATCAGGAA+AGG | + | 37913:7298-7317 | None:intergenic | 50.0% | |
| CTCCAAGGGATCCAGTATCT+CGG | + | 37913:7314-7333 | None:intergenic | 50.0% | |
| GACACAAGAGGTTCGAGGTT+TGG | - | 37913:7082-7101 | MS.gene26319:CDS | 50.0% | |
| GCATCATGAACTTCGGGATG+AGG | + | 37913:7493-7512 | None:intergenic | 50.0% | |
| GCGTCTAAGTTACAACGGAC+AGG | - | 37913:7195-7214 | MS.gene26319:CDS | 50.0% | |
| GCTGTTGCCATGGGTAACAA+TGG | - | 37913:7655-7674 | MS.gene26319:intron | 50.0% | |
| GGCATCTGTAAGTAGGATGG+TGG | + | 37913:7930-7949 | None:intergenic | 50.0% | |
| GTGAAAACATGGGTGCAGGT+GGG | - | 37913:7677-7696 | MS.gene26319:intron | 50.0% | |
| GTTGTCTTCATCGGATGACG+AGG | + | 37913:7356-7375 | None:intergenic | 50.0% | |
| TCATGAACTTCGGGATGAGG+AGG | + | 37913:7490-7509 | None:intergenic | 50.0% | |
| TCCTTCCACTTGATCCTGCA+CGG | + | 37913:7968-7987 | None:intergenic | 50.0% | |
| TCGGGATGAGGAGGAGAATT+GGG | + | 37913:7481-7500 | None:intergenic | 50.0% | |
| TTCAACTCCTGTGGCAGAAC+CGG | - | 37913:7432-7451 | MS.gene26319:intron | 50.0% | |
| TTCGGGATGAGGAGGAGAAT+TGG | + | 37913:7482-7501 | None:intergenic | 50.0% | |
| ! | ATTTGTTACGGAGAACCGGC+TGG | - | 37913:8036-8055 | MS.gene26319:intron | 50.0% |
| ! | CCTGTCTCCCTTTTGGTATC+CGG | - | 37913:6415-6434 | MS.gene26319:CDS | 50.0% |
| ! | GGACGCGGACCTTTTTCTAT+AGG | - | 37913:7125-7144 | MS.gene26319:CDS | 50.0% |
| !! | AGGCGGATTTTGGGCATCAT+CGG | + | 37913:7536-7555 | None:intergenic | 50.0% |
| !! | TCTGTAAGTAGGATGGTGGG+CGG | + | 37913:7926-7945 | None:intergenic | 50.0% |
| CCAGTATCTCGGCAGCAATC+AGG | + | 37913:7303-7322 | None:intergenic | 55.0% | |
| CCGGATACCAAAAGGGAGAC+AGG | + | 37913:6418-6437 | None:intergenic | 55.0% | |
| CCTCTCACGAACCGTTAGCT+CGG | + | 37913:7257-7276 | None:intergenic | 55.0% | |
| CCTGATTGCTGCCGAGATAC+TGG | - | 37913:7300-7319 | MS.gene26319:CDS | 55.0% | |
| CGAGATACTGGATCCCTTGG+AGG | - | 37913:7312-7331 | MS.gene26319:CDS | 55.0% | |
| CGGCAGCAATCAGGAAAGGA+GGG | + | 37913:7294-7313 | None:intergenic | 55.0% | |
| GAAATGCCGCTGGAGGAAGA+TGG | + | 37913:7997-8016 | None:intergenic | 55.0% | |
| GATGCTGCTGCTGTTGCCAT+GGG | - | 37913:7646-7665 | MS.gene26319:intron | 55.0% | |
| GCACGGAAAGTCAAAGCCCA+TGG | + | 37913:7951-7970 | None:intergenic | 55.0% | |
| GCAGTATGAGACCTAACGGC+TGG | + | 37913:7177-7196 | None:intergenic | 55.0% | |
| GGACAGGGGCTGTTCTACAT+GGG | - | 37913:7211-7230 | MS.gene26319:CDS | 55.0% | |
| GGTGAAAACATGGGTGCAGG+TGG | - | 37913:7676-7695 | MS.gene26319:intron | 55.0% | |
| GGTGCAACATCAACATCCGC+TGG | + | 37913:7460-7479 | None:intergenic | 55.0% | |
| GGTTCTGCCACAGGAGTTGA+AGG | + | 37913:7433-7452 | None:intergenic | 55.0% | |
| GTATGAGACCTAACGGCTGG+TGG | + | 37913:7174-7193 | None:intergenic | 55.0% | |
| GTATGCTAGGGAAGCTCGGA+TGG | - | 37913:8111-8130 | MS.gene26319:CDS | 55.0% | |
| TCATAGGAAGTGAGGGGACG+CGG | - | 37913:7110-7129 | MS.gene26319:CDS | 55.0% | |
| TCCGTGCAGGATCAAGTGGA+AGG | - | 37913:7964-7983 | MS.gene26319:intron | 55.0% | |
| TCCTCCAGCGGCATTTCTCA+GGG | - | 37913:8000-8019 | MS.gene26319:intron | 55.0% | |
| TCGGCAGCAATCAGGAAAGG+AGG | + | 37913:7295-7314 | None:intergenic | 55.0% | |
| TCTGCCACAGGAGTTGAAGG+AGG | + | 37913:7430-7449 | None:intergenic | 55.0% | |
| TGATGCTGCTGCTGTTGCCA+TGG | - | 37913:7645-7664 | MS.gene26319:intron | 55.0% | |
| TGCACCTCCTTCAACTCCTG+TGG | - | 37913:7423-7442 | MS.gene26319:intron | 55.0% | |
| TGCCGAGATACTGGATCCCT+TGG | - | 37913:7309-7328 | MS.gene26319:CDS | 55.0% | |
| TGGGCATCATCGGCATTGTG+TGG | + | 37913:7526-7545 | None:intergenic | 55.0% | |
| TTCCTCCAGCGGCATTTCTC+AGG | - | 37913:7999-8018 | MS.gene26319:intron | 55.0% | |
| ! | ACAGGAGTTGAAGGAGGTGC+AGG | + | 37913:7424-7443 | None:intergenic | 55.0% |
| ! | CACTTTTCCCAACTGCCAGC+CGG | + | 37913:8054-8073 | None:intergenic | 55.0% |
| ! | TGGGCTTTGACTTTCCGTGC+AGG | - | 37913:7951-7970 | MS.gene26319:intron | 55.0% |
| CAGAGGCCATCTTCCTCCAG+CGG | - | 37913:7988-8007 | MS.gene26319:intron | 60.0% | |
| CATGGGTGCAGGTGGGAATG+TGG | - | 37913:7684-7703 | MS.gene26319:intron | 60.0% | |
| CCGAGCTAACGGTTCGTGAG+AGG | - | 37913:7254-7273 | MS.gene26319:CDS | 60.0% | |
| CCTCCAGCGGCATTTCTCAG+GGG | - | 37913:8001-8020 | MS.gene26319:intron | 60.0% | |
| CGGACAGGGGCTGTTCTACA+TGG | - | 37913:7210-7229 | MS.gene26319:CDS | 60.0% | |
| GAGCACTTCCACCAGCCGTT+AGG | - | 37913:7163-7182 | MS.gene26319:CDS | 60.0% | |
| TGTGGCAGAACCGGATCCAG+CGG | - | 37913:7441-7460 | MS.gene26319:intron | 60.0% | |
| ! | GGAGTTGAAGGAGGTGCAGG+AGG | + | 37913:7421-7440 | None:intergenic | 60.0% |
| ACGGAGAACCGGCTGGCAGT+TGG | - | 37913:8043-8062 | MS.gene26319:intron | 65.0% | |
| CAGCGGCATTTCTCAGGGGC+GGG | - | 37913:8005-8024 | MS.gene26319:intron | 65.0% | |
| CCAGCGGCATTTCTCAGGGG+CGG | - | 37913:8004-8023 | MS.gene26319:intron | 65.0% | |
| CCCCTGAGAAATGCCGCTGG+AGG | + | 37913:8004-8023 | None:intergenic | 65.0% | |
| CGGAGAACCGGCTGGCAGTT+GGG | - | 37913:8044-8063 | MS.gene26319:intron | 65.0% | |
| GCTGGATCCGGTTCTGCCAC+AGG | + | 37913:7442-7461 | None:intergenic | 65.0% | |
| CCGCCCCTGAGAAATGCCGC+TGG | + | 37913:8007-8026 | None:intergenic | 70.0% | |
| CGGCATTTCTCAGGGGCGGG+TGG | - | 37913:8008-8027 | MS.gene26319:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37913 | gene | 6273 | 8141 | 6273 | ID=MS.gene26319 |
| 37913 | mRNA | 6273 | 8141 | 6273 | ID=MS.gene26319.t1;Parent=MS.gene26319 |
| 37913 | exon | 8091 | 8141 | 8091 | ID=MS.gene26319.t1.exon1;Parent=MS.gene26319.t1 |
| 37913 | CDS | 8091 | 8141 | 8091 | ID=cds.MS.gene26319.t1;Parent=MS.gene26319.t1 |
| 37913 | exon | 6640 | 7404 | 6640 | ID=MS.gene26319.t1.exon2;Parent=MS.gene26319.t1 |
| 37913 | CDS | 6640 | 7404 | 6640 | ID=cds.MS.gene26319.t1;Parent=MS.gene26319.t1 |
| 37913 | exon | 6273 | 6536 | 6273 | ID=MS.gene26319.t1.exon3;Parent=MS.gene26319.t1 |
| 37913 | CDS | 6273 | 6536 | 6273 | ID=cds.MS.gene26319.t1;Parent=MS.gene26319.t1 |
| Gene Sequence |
| Protein sequence |
