Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26585.t1 | XP_024634403.1 | 67.9 | 84 | 26 | 1 | 1 | 84 | 1 | 83 | 6.30E-24 | 120.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26585.t1 | Q3E7I5 | 44.6 | 56 | 30 | 1 | 9 | 63 | 42 | 97 | 1.8e-06 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26585.t1 | A0A396H640 | 52.0 | 100 | 38 | 1 | 1 | 90 | 1 | 100 | 1.1e-22 | 115.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene26585.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26585.t1 | MTR_7g074790 | 68.831 | 77 | 24 | 0 | 1 | 77 | 1 | 77 | 5.88e-33 | 115 |
| MS.gene26585.t1 | MTR_7g061980 | 58.750 | 80 | 33 | 0 | 1 | 80 | 1 | 80 | 6.61e-30 | 103 |
| MS.gene26585.t1 | MTR_5g039760 | 49.000 | 100 | 41 | 1 | 1 | 90 | 1 | 100 | 9.63e-30 | 105 |
| MS.gene26585.t1 | MTR_4g069880 | 60.294 | 68 | 27 | 0 | 1 | 68 | 1 | 68 | 3.84e-21 | 80.1 |
| MS.gene26585.t1 | MTR_4g102720 | 47.619 | 63 | 33 | 0 | 7 | 69 | 4 | 66 | 1.18e-14 | 67.4 |
| MS.gene26585.t1 | MTR_0137s0040 | 40.659 | 91 | 45 | 3 | 2 | 91 | 28 | 110 | 1.84e-14 | 64.3 |
| MS.gene26585.t1 | MTR_6g017200 | 45.455 | 66 | 35 | 1 | 10 | 74 | 58 | 123 | 1.20e-12 | 60.8 |
| MS.gene26585.t1 | MTR_1g057920 | 46.667 | 60 | 31 | 1 | 10 | 68 | 34 | 93 | 1.21e-11 | 57.4 |
| MS.gene26585.t1 | MTR_5g015480 | 39.560 | 91 | 42 | 3 | 4 | 84 | 53 | 140 | 4.59e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAGTTTACGTGCGGGCTT+TGG | 0.344757 | 4.1:+22635919 | MS.gene26585:CDS |
| TGGAATCTTCAAATGTTGAT+TGG | 0.360826 | 4.1:+22635836 | MS.gene26585:CDS |
| TTGGAATGGAATTTGACAAT+AGG | 0.381885 | 4.1:+22635869 | MS.gene26585:CDS |
| TGGAATGGAATTTGACAATA+GGG | 0.410954 | 4.1:+22635870 | MS.gene26585:CDS |
| TCAAATTCCATTCCAAGTCT+TGG | 0.427302 | 4.1:-22635862 | None:intergenic |
| ATGTAGGTTTGTTTGTTGTA+AGG | 0.436082 | 4.1:+22635990 | MS.gene26585:CDS |
| CTGACGCCGTCTCCTCTCTC+TGG | 0.440485 | 4.1:-22637054 | None:intergenic |
| GACAAGAGGAGGTGAATTGC+TGG | 0.445034 | 4.1:+22636984 | MS.gene26585:CDS |
| TGAATTGCTGGTGGCGGCGT+CGG | 0.474650 | 4.1:+22636996 | MS.gene26585:CDS |
| AGGTTTGTTTGTTGTAAGGA+AGG | 0.474808 | 4.1:+22635994 | MS.gene26585:CDS |
| GTTGATTGGAAGCCAAGACT+TGG | 0.497681 | 4.1:+22635850 | MS.gene26585:CDS |
| GAAGCGACGCGTTGCAGCAA+AGG | 0.506272 | 4.1:+22637022 | MS.gene26585:CDS |
| TTGGAAGCCAAGACTTGGAA+TGG | 0.513444 | 4.1:+22635855 | MS.gene26585:CDS |
| GGTTTGTTTGTTGTAAGGAA+GGG | 0.530392 | 4.1:+22635995 | MS.gene26585:CDS |
| ACGGTAGTATATCGTCATGT+AGG | 0.560813 | 4.1:+22635974 | MS.gene26585:CDS |
| GAGGAGACGGCGTCAGCTAG+AGG | 0.567502 | 4.1:+22637061 | MS.gene26585:CDS |
| AGGAGGTGAATTGCTGGTGG+CGG | 0.570734 | 4.1:+22636990 | MS.gene26585:CDS |
| ACGCCGTCTCCTCTCTCTGG+TGG | 0.574148 | 4.1:-22637051 | None:intergenic |
| ACGTAATGCAAACAAGAAGA+AGG | 0.577432 | 4.1:+22635951 | MS.gene26585:CDS |
| TTTGTTGTAAGGAAGGGCTA+CGG | 0.581016 | 4.1:+22636001 | MS.gene26585:CDS |
| GTTGCTTATAGTTTACGTGC+GGG | 0.585073 | 4.1:+22635913 | MS.gene26585:CDS |
| TGTTGCTTATAGTTTACGTG+CGG | 0.599027 | 4.1:+22635912 | MS.gene26585:CDS |
| CGACGCGTTGCAGCAAAGGA+CGG | 0.602009 | 4.1:+22637026 | MS.gene26585:CDS |
| GAATAGATATCGAAGACAAG+AGG | 0.613423 | 4.1:+22636970 | MS.gene26585:intron |
| GCACCACCAGAGAGAGGAGA+CGG | 0.627282 | 4.1:+22637048 | MS.gene26585:CDS |
| AGGACGGCACCACCAGAGAG+AGG | 0.638836 | 4.1:+22637042 | MS.gene26585:CDS |
| TAGATATCGAAGACAAGAGG+AGG | 0.644754 | 4.1:+22636973 | MS.gene26585:intron |
| AATGCAAACAAGAAGAAGGA+CGG | 0.678659 | 4.1:+22635955 | MS.gene26585:CDS |
| AAGAGGAGGTGAATTGCTGG+TGG | 0.682948 | 4.1:+22636987 | MS.gene26585:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAATTCATAATAGAAAAT+GGG | - | chr4.1:22636200-22636219 | None:intergenic | 15.0% |
| !!! | AATTGATTTGGAATTTAATT+GGG | - | chr4.1:22636852-22636871 | None:intergenic | 15.0% |
| !! | AATTGTTATCTCTTAAATAG+TGG | - | chr4.1:22636899-22636918 | None:intergenic | 20.0% |
| !! | GAAAAACATACAATATTGTT+AGG | - | chr4.1:22636812-22636831 | None:intergenic | 20.0% |
| !! | GAGAAATTCATAATAGAAAA+TGG | - | chr4.1:22636201-22636220 | None:intergenic | 20.0% |
| !! | TACAATATTGTTAGGAAAAT+TGG | - | chr4.1:22636804-22636823 | None:intergenic | 20.0% |
| !!! | ATTGATTTGGAATTTAATTG+GGG | - | chr4.1:22636851-22636870 | None:intergenic | 20.0% |
| !!! | GAATTGATTTGGAATTTAAT+TGG | - | chr4.1:22636853-22636872 | None:intergenic | 20.0% |
| ! | AAAGTAACTATTTCATCATG+TGG | - | chr4.1:22636087-22636106 | None:intergenic | 25.0% |
| ! | ACATGATGAAATAGTTACTT+TGG | + | chr4.1:22636086-22636105 | MS.gene26585:intron | 25.0% |
| ! | ATAATAGAAAATGGGAAGAA+TGG | - | chr4.1:22636192-22636211 | None:intergenic | 25.0% |
| ! | CAAGGAAAAATAGTTAGAAT+AGG | + | chr4.1:22636048-22636067 | MS.gene26585:CDS | 25.0% |
| ! | TGTAGAAATGGAGATAAAAA+GGG | - | chr4.1:22636291-22636310 | None:intergenic | 25.0% |
| ! | TTGTAGAAATGGAGATAAAA+AGG | - | chr4.1:22636292-22636311 | None:intergenic | 25.0% |
| ! | TTTGAGTAGTAGAATTGATT+TGG | - | chr4.1:22636864-22636883 | None:intergenic | 25.0% |
| !! | TAGTTACTTTGGATTCTATT+TGG | + | chr4.1:22636097-22636116 | MS.gene26585:intron | 25.0% |
| !!! | AATAATGATCTTCTGAAACA+AGG | + | chr4.1:22636331-22636350 | MS.gene26585:intron | 25.0% |
| !!! | AGTTTTGGAATTAGAGATTA+GGG | - | chr4.1:22636417-22636436 | None:intergenic | 25.0% |
| !!! | TAGGGTTTTTTTTATTGAGT+TGG | - | chr4.1:22636399-22636418 | None:intergenic | 25.0% |
| !!! | TTTATTGAGTTGGTGTATTT+TGG | - | chr4.1:22636389-22636408 | None:intergenic | 25.0% |
| AAACAAACAACTACTTTAGC+AGG | + | chr4.1:22636517-22636536 | MS.gene26585:intron | 30.0% | |
| AAATCAGAACTTGTGAAAAG+CGG | + | chr4.1:22636759-22636778 | MS.gene26585:intron | 30.0% | |
| GTTTCAGAAGATCATTATTG+CGG | - | chr4.1:22636330-22636349 | None:intergenic | 30.0% | |
| TATTCATGTTATATAGCCGT+AGG | - | chr4.1:22636482-22636501 | None:intergenic | 30.0% | |
| TATTGTTAGGAAAATTGGTC+TGG | - | chr4.1:22636799-22636818 | None:intergenic | 30.0% | |
| TCTCTAATTCCAAAACTGAT+AGG | + | chr4.1:22636420-22636439 | MS.gene26585:intron | 30.0% | |
| TGCTAAAGTAGTTGTTTGTT+TGG | - | chr4.1:22636518-22636537 | None:intergenic | 30.0% | |
| TGGAATCTTCAAATGTTGAT+TGG | + | chr4.1:22635836-22635855 | MS.gene26585:CDS | 30.0% | |
| TGGAATGGAATTTGACAATA+GGG | + | chr4.1:22635870-22635889 | MS.gene26585:CDS | 30.0% | |
| TTGGAATGGAATTTGACAAT+AGG | + | chr4.1:22635869-22635888 | MS.gene26585:CDS | 30.0% | |
| ! | ATTTGGAATTTAATTGGGGT+TGG | - | chr4.1:22636847-22636866 | None:intergenic | 30.0% |
| ! | TTCTGATTTTCAATCTGCTA+TGG | - | chr4.1:22636748-22636767 | None:intergenic | 30.0% |
| ! | TTGTGCAGTTGAATCAATTT+TGG | - | chr4.1:22636933-22636952 | None:intergenic | 30.0% |
| !! | ATGTAGGTTTGTTTGTTGTA+AGG | + | chr4.1:22635990-22636009 | MS.gene26585:CDS | 30.0% |
| !! | TAGTTGTTTGTTTGGCTTTA+TGG | - | chr4.1:22636510-22636529 | None:intergenic | 30.0% |
| !!! | CAGTTTTGGAATTAGAGATT+AGG | - | chr4.1:22636418-22636437 | None:intergenic | 30.0% |
| AATGCAAACAAGAAGAAGGA+CGG | + | chr4.1:22635955-22635974 | MS.gene26585:CDS | 35.0% | |
| ACAATGAGAAAGAATTGCTC+AGG | - | chr4.1:22636168-22636187 | None:intergenic | 35.0% | |
| ACGTAATGCAAACAAGAAGA+AGG | + | chr4.1:22635951-22635970 | MS.gene26585:CDS | 35.0% | |
| AGCTTTCAAAATCAAGCTCT+TGG | - | chr4.1:22636679-22636698 | None:intergenic | 35.0% | |
| GAATAGATATCGAAGACAAG+AGG | + | chr4.1:22636970-22636989 | MS.gene26585:intron | 35.0% | |
| GAGAAAAAAGATGCACATCA+AGG | + | chr4.1:22636030-22636049 | MS.gene26585:CDS | 35.0% | |
| GCTTTCAAAATCAAGCTCTT+GGG | - | chr4.1:22636678-22636697 | None:intergenic | 35.0% | |
| TCAAATTCCATTCCAAGTCT+TGG | - | chr4.1:22635865-22635884 | None:intergenic | 35.0% | |
| TGCTGTTACAGTTGTAGAAA+TGG | - | chr4.1:22636303-22636322 | None:intergenic | 35.0% | |
| ! | TGTTGCTTATAGTTTACGTG+CGG | + | chr4.1:22635912-22635931 | MS.gene26585:CDS | 35.0% |
| !! | AGGTTTGTTTGTTGTAAGGA+AGG | + | chr4.1:22635994-22636013 | MS.gene26585:CDS | 35.0% |
| !! | GGTTTGTTTGTTGTAAGGAA+GGG | + | chr4.1:22635995-22636014 | MS.gene26585:CDS | 35.0% |
| !! | TCTCTAGTTCCTATCAGTTT+TGG | - | chr4.1:22636432-22636451 | None:intergenic | 35.0% |
| !!! | GTTGGTGTATTTTGGTGTAT+TGG | - | chr4.1:22636381-22636400 | None:intergenic | 35.0% |
| AAACAACAGCAGCACCTAAA+AGG | + | chr4.1:22636543-22636562 | MS.gene26585:intron | 40.0% | |
| ACGGTAGTATATCGTCATGT+AGG | + | chr4.1:22635974-22635993 | MS.gene26585:CDS | 40.0% | |
| ATAGGAACTAGAGAACCGAA+TGG | + | chr4.1:22636438-22636457 | MS.gene26585:intron | 40.0% | |
| CAAAATCAAGCTCTTGGGAA+AGG | - | chr4.1:22636673-22636692 | None:intergenic | 40.0% | |
| CTCTTGGGAAAGGATTTGAT+GGG | - | chr4.1:22636663-22636682 | None:intergenic | 40.0% | |
| GAGTGATTACTGCAACAAGT+TGG | - | chr4.1:22636146-22636165 | None:intergenic | 40.0% | |
| GTTGCTTATAGTTTACGTGC+GGG | + | chr4.1:22635913-22635932 | MS.gene26585:CDS | 40.0% | |
| TAGATATCGAAGACAAGAGG+AGG | + | chr4.1:22636973-22636992 | MS.gene26585:intron | 40.0% | |
| TCTTGGGAAAGGATTTGATG+GGG | - | chr4.1:22636662-22636681 | None:intergenic | 40.0% | |
| ! | TGATTACTGCAACAAGTTGG+AGG | - | chr4.1:22636143-22636162 | None:intergenic | 40.0% |
| ! | TTTGTTGTAAGGAAGGGCTA+CGG | + | chr4.1:22636001-22636020 | MS.gene26585:CDS | 40.0% |
| !! | AAAAGGGTACTGACTCAACT+TGG | - | chr4.1:22636275-22636294 | None:intergenic | 40.0% |
| !!! | GGTGTATTTTGGTGTATTGG+TGG | - | chr4.1:22636378-22636397 | None:intergenic | 40.0% |
| !!! | TTTAGGTGCTGCTGTTGTTT+TGG | - | chr4.1:22636543-22636562 | None:intergenic | 40.0% |
| AATCGCGCAGTTATTCTCAC+TGG | - | chr4.1:22636234-22636253 | None:intergenic | 45.0% | |
| CTATGGACTCAGTATGCAGA+AGG | - | chr4.1:22636731-22636750 | None:intergenic | 45.0% | |
| CTTGGGAAAGGATTTGATGG+GGG | - | chr4.1:22636661-22636680 | None:intergenic | 45.0% | |
| GCTCTTGGGAAAGGATTTGA+TGG | - | chr4.1:22636664-22636683 | None:intergenic | 45.0% | |
| GTAGCGCTAGAAAACACCTA+CGG | + | chr4.1:22636463-22636482 | MS.gene26585:intron | 45.0% | |
| GTTGATTGGAAGCCAAGACT+TGG | + | chr4.1:22635850-22635869 | MS.gene26585:CDS | 45.0% | |
| TATAGTTTACGTGCGGGCTT+TGG | + | chr4.1:22635919-22635938 | MS.gene26585:CDS | 45.0% | |
| TTGGAAGCCAAGACTTGGAA+TGG | + | chr4.1:22635855-22635874 | MS.gene26585:CDS | 45.0% | |
| ! | GGTGCAAGGTCAAGTGTTTT+AGG | - | chr4.1:22636357-22636376 | None:intergenic | 45.0% |
| ! | TTTCTAGCGCTACTGCCATT+CGG | - | chr4.1:22636456-22636475 | None:intergenic | 45.0% |
| !! | TTTGGTGTATTGGTGGTGCA+AGG | - | chr4.1:22636371-22636390 | None:intergenic | 45.0% |
| AAGAGGAGGTGAATTGCTGG+TGG | + | chr4.1:22636987-22637006 | MS.gene26585:CDS | 50.0% | |
| CTGCTGTTGTGTAGACAGCA+TGG | - | chr4.1:22636576-22636595 | None:intergenic | 50.0% | |
| GACAAGAGGAGGTGAATTGC+TGG | + | chr4.1:22636984-22637003 | MS.gene26585:CDS | 50.0% | |
| GGACTCAGTATGCAGAAGGA+TGG | - | chr4.1:22636727-22636746 | None:intergenic | 50.0% | |
| ! | AGCATGGCTGTGAGCCTTTT+AGG | - | chr4.1:22636560-22636579 | None:intergenic | 50.0% |
| !! | GGGTACTGACTCAACTTGGT+TGG | - | chr4.1:22636271-22636290 | None:intergenic | 50.0% |
| !!! | GCTGGATTGGTGGCAGTTTT+AGG | - | chr4.1:22636624-22636643 | None:intergenic | 50.0% |
| !!! | GGATTGGTGGCAGTTTTAGG+TGG | - | chr4.1:22636621-22636640 | None:intergenic | 50.0% |
| !!! | TTGGTGGCAGTTTTAGGTGG+TGG | - | chr4.1:22636618-22636637 | None:intergenic | 50.0% |
| AGGAGGTGAATTGCTGGTGG+CGG | + | chr4.1:22636990-22637009 | MS.gene26585:CDS | 55.0% | |
| TTTGATGGGGGACTTGACCG+TGG | - | chr4.1:22636649-22636668 | None:intergenic | 55.0% | |
| !!! | GTGGCAGTTTTAGGTGGTGG+TGG | - | chr4.1:22636615-22636634 | None:intergenic | 55.0% |
| !!! | GTTTTAGGTGGTGGTGGTGC+TGG | - | chr4.1:22636609-22636628 | None:intergenic | 55.0% |
| CGACGCGTTGCAGCAAAGGA+CGG | + | chr4.1:22637026-22637045 | MS.gene26585:CDS | 60.0% | |
| CTTGACCGTGGTGGCTGGAT+TGG | - | chr4.1:22636637-22636656 | None:intergenic | 60.0% | |
| GAAGCGACGCGTTGCAGCAA+AGG | + | chr4.1:22637022-22637041 | MS.gene26585:CDS | 60.0% | |
| GCACCACCAGAGAGAGGAGA+CGG | + | chr4.1:22637048-22637067 | MS.gene26585:CDS | 60.0% | |
| TGCCACCAATCCAGCCACCA+CGG | + | chr4.1:22636629-22636648 | MS.gene26585:intron | 60.0% | |
| !! | TGAATTGCTGGTGGCGGCGT+CGG | + | chr4.1:22636996-22637015 | MS.gene26585:CDS | 60.0% |
| !! | TGGTGGTGGTGCTGGTTTCG+TGG | - | chr4.1:22636601-22636620 | None:intergenic | 60.0% |
| ACGCCGTCTCCTCTCTCTGG+TGG | - | chr4.1:22637054-22637073 | None:intergenic | 65.0% | |
| AGGACGGCACCACCAGAGAG+AGG | + | chr4.1:22637042-22637061 | MS.gene26585:CDS | 65.0% | |
| CTGACGCCGTCTCCTCTCTC+TGG | - | chr4.1:22637057-22637076 | None:intergenic | 65.0% | |
| GATGGGGGACTTGACCGTGG+TGG | - | chr4.1:22636646-22636665 | None:intergenic | 65.0% | |
| ! | GACCGTGGTGGCTGGATTGG+TGG | - | chr4.1:22636634-22636653 | None:intergenic | 65.0% |
| GGGGACTTGACCGTGGTGGC+TGG | - | chr4.1:22636642-22636661 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 22635835 | 22637080 | 22635835 | ID=MS.gene26585 |
| chr4.1 | mRNA | 22635835 | 22637080 | 22635835 | ID=MS.gene26585.t1;Parent=MS.gene26585 |
| chr4.1 | exon | 22635835 | 22636071 | 22635835 | ID=MS.gene26585.t1.exon1;Parent=MS.gene26585.t1 |
| chr4.1 | CDS | 22635835 | 22636071 | 22635835 | ID=cds.MS.gene26585.t1;Parent=MS.gene26585.t1 |
| chr4.1 | exon | 22636976 | 22637080 | 22636976 | ID=MS.gene26585.t1.exon2;Parent=MS.gene26585.t1 |
| chr4.1 | CDS | 22636976 | 22637080 | 22636976 | ID=cds.MS.gene26585.t1;Parent=MS.gene26585.t1 |
| Gene Sequence |
| Protein sequence |
