Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26663.t1 | XP_013456955.1 | 68.9 | 74 | 2 | 1 | 4 | 56 | 22 | 95 | 4.20E-17 | 97.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26663.t1 | Q9FFH1 | 59.2 | 49 | 20 | 0 | 7 | 55 | 32 | 80 | 8.7e-11 | 67.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26663.t1 | A0A072UPN4 | 68.9 | 74 | 2 | 1 | 4 | 56 | 22 | 95 | 3.1e-17 | 97.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene26663.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26663.t1 | MTR_4g088375 | 68.000 | 75 | 3 | 1 | 3 | 56 | 21 | 95 | 4.14e-26 | 101 |
| MS.gene26663.t1 | MTR_4g088375 | 68.000 | 75 | 3 | 1 | 3 | 56 | 21 | 95 | 4.26e-26 | 101 |
| MS.gene26663.t1 | MTR_4g088375 | 68.000 | 75 | 3 | 1 | 3 | 56 | 21 | 95 | 4.56e-26 | 101 |
| MS.gene26663.t1 | MTR_2g014490 | 76.744 | 43 | 10 | 0 | 13 | 55 | 61 | 103 | 1.78e-17 | 76.6 |
| MS.gene26663.t1 | MTR_2g014490 | 76.744 | 43 | 10 | 0 | 13 | 55 | 61 | 103 | 2.02e-17 | 76.6 |
| MS.gene26663.t1 | MTR_4g078860 | 68.293 | 41 | 13 | 0 | 13 | 53 | 40 | 80 | 3.26e-14 | 67.4 |
| MS.gene26663.t1 | MTR_4g078860 | 68.293 | 41 | 13 | 0 | 13 | 53 | 40 | 80 | 3.36e-14 | 67.4 |
| MS.gene26663.t1 | MTR_4g078860 | 68.293 | 41 | 13 | 0 | 13 | 53 | 40 | 80 | 4.53e-13 | 64.3 |
| MS.gene26663.t1 | MTR_4g078860 | 68.293 | 41 | 13 | 0 | 13 | 53 | 40 | 80 | 4.65e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26663.t1 | AT5G44180 | 57.692 | 52 | 22 | 0 | 7 | 58 | 32 | 83 | 5.91e-15 | 69.7 |
| MS.gene26663.t1 | AT5G44180 | 57.692 | 52 | 22 | 0 | 7 | 58 | 32 | 83 | 6.05e-15 | 69.7 |
| MS.gene26663.t1 | AT5G44180 | 57.692 | 52 | 22 | 0 | 7 | 58 | 32 | 83 | 6.05e-15 | 69.7 |
| MS.gene26663.t1 | AT1G28420 | 69.048 | 42 | 13 | 0 | 14 | 55 | 61 | 102 | 1.45e-14 | 68.6 |
Find 53 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAGATTTGTCTGAGAAAT+TGG | 0.144437 | 4.1:-21966182 | MS.gene26663:CDS |
| TCCGTGGCGGCACACCTTCT+TGG | 0.182300 | 4.1:+21966090 | None:intergenic |
| CCGTGGCGGCACACCTTCTT+GGG | 0.314843 | 4.1:+21966091 | None:intergenic |
| ATAGATTTGTCTGAGAAATT+GGG | 0.322970 | 4.1:-21966181 | MS.gene26663:CDS |
| TATGACTCAGGGTCGGGTTC+AGG | 0.352859 | 4.1:-21965996 | MS.gene26663:CDS |
| GCCTGTGGTTCAGCCTTTGC+CGG | 0.356317 | 4.1:-21966066 | MS.gene26663:CDS |
| TCGGGTTCAGGTTCGAGTCC+GGG | 0.361328 | 4.1:-21965984 | MS.gene26663:CDS |
| GTCGGGTTCAGGTTCGAGTC+CGG | 0.384281 | 4.1:-21965985 | MS.gene26663:CDS |
| GTCTGAGAAATTGGGGTTGA+CGG | 0.392863 | 4.1:-21966173 | MS.gene26663:CDS |
| AGGCTTACTCTGCCCCTCAT+TGG | 0.410523 | 4.1:+21966413 | None:intergenic |
| TTCAGGTTCGAGTCCGGGGT+TGG | 0.426233 | 4.1:-21965979 | MS.gene26663:CDS |
| TCAGCCTTTGCCGGATTCCC+CGG | 0.426296 | 4.1:-21966057 | MS.gene26663:CDS |
| TGATCATCCACCGGGGAATC+CGG | 0.426382 | 4.1:+21966047 | None:intergenic |
| TGCAGATGTGGTTCTGTCAT+AGG | 0.441916 | 4.1:-21966141 | MS.gene26663:CDS |
| TAGGAGGTTGAAGGATAAGA+AGG | 0.444980 | 4.1:-21966122 | MS.gene26663:CDS |
| TCTATCCTCACCGGCTCTGA+AGG | 0.456529 | 4.1:+21966199 | None:intergenic |
| GCATTCCGCGGCTCCAACCC+CGG | 0.486997 | 4.1:+21965966 | None:intergenic |
| CCCAAGAAGGTGTGCCGCCA+CGG | 0.491039 | 4.1:-21966091 | MS.gene26663:CDS |
| CGGATCGGCAGTTGCAGATG+TGG | 0.492519 | 4.1:-21966153 | MS.gene26663:CDS |
| AAAATTGCTGTTTCCAATGA+GGG | 0.499940 | 4.1:-21966426 | MS.gene26663:CDS |
| TAGATTTGTCTGAGAAATTG+GGG | 0.508540 | 4.1:-21966180 | MS.gene26663:CDS |
| TCCGGCAAAGGCTGAACCAC+AGG | 0.511181 | 4.1:+21966065 | None:intergenic |
| GAATGCAATGGCACGAGTGT+TGG | 0.513830 | 4.1:-21965949 | MS.gene26663:CDS |
| CGGGTTCAGGTTCGAGTCCG+GGG | 0.520346 | 4.1:-21965983 | MS.gene26663:CDS |
| CCCATCCTGTGATCATCCAC+CGG | 0.522966 | 4.1:+21966038 | None:intergenic |
| CCATCCTGTGATCATCCACC+GGG | 0.523980 | 4.1:+21966039 | None:intergenic |
| CGAGTCCGGGGTTGGAGCCG+CGG | 0.531188 | 4.1:-21965971 | MS.gene26663:CDS |
| AATGAGTATGACTCAGGGTC+GGG | 0.532240 | 4.1:-21966002 | MS.gene26663:CDS |
| CCGGTGGATGATCACAGGAT+GGG | 0.536456 | 4.1:-21966038 | MS.gene26663:CDS |
| AGAAATTGGGGTTGACGGAT+CGG | 0.536879 | 4.1:-21966168 | MS.gene26663:CDS |
| CCCGGTGGATGATCACAGGA+TGG | 0.538596 | 4.1:-21966039 | MS.gene26663:CDS |
| GGCTGAACCACAGGCTTCCG+TGG | 0.541562 | 4.1:+21966074 | None:intergenic |
| CATGGTAATGAGTATGACTC+AGG | 0.548652 | 4.1:-21966008 | MS.gene26663:CDS |
| CGGTGGATGATCACAGGATG+GGG | 0.560830 | 4.1:-21966037 | MS.gene26663:CDS |
| CACAGGATGGGGTTTGAGCA+TGG | 0.571035 | 4.1:-21966026 | MS.gene26663:CDS |
| GTTGGAGCCGCGGAATGCAA+TGG | 0.571805 | 4.1:-21965961 | MS.gene26663:CDS |
| TCCACCGGGGAATCCGGCAA+AGG | 0.578330 | 4.1:+21966053 | None:intergenic |
| ATTCCCCGGTGGATGATCAC+AGG | 0.578456 | 4.1:-21966043 | MS.gene26663:CDS |
| CTATCCTCACCGGCTCTGAA+GGG | 0.588722 | 4.1:+21966200 | None:intergenic |
| GGATAATTACCCTTCAGAGC+CGG | 0.592084 | 4.1:-21966209 | MS.gene26663:CDS |
| GAAGGAGTCTGCTCCCAAGA+AGG | 0.597682 | 4.1:-21966104 | MS.gene26663:CDS |
| GTTCTGTCATAGGAGGTTGA+AGG | 0.597689 | 4.1:-21966131 | MS.gene26663:CDS |
| TAATGAGTATGACTCAGGGT+CGG | 0.605502 | 4.1:-21966003 | MS.gene26663:CDS |
| GCCTTTGCCGGATTCCCCGG+TGG | 0.611126 | 4.1:-21966054 | MS.gene26663:CDS |
| TGTGCCGCCACGGAAGCCTG+TGG | 0.618031 | 4.1:-21966081 | MS.gene26663:CDS |
| TCAGACAAATCTATCCTCAC+CGG | 0.619274 | 4.1:+21966190 | None:intergenic |
| AAATTGCTGTTTCCAATGAG+GGG | 0.624303 | 4.1:-21966425 | MS.gene26663:CDS |
| AGATGTGGTTCTGTCATAGG+AGG | 0.638175 | 4.1:-21966138 | MS.gene26663:CDS |
| ACTCGTGCCATTGCATTCCG+CGG | 0.661434 | 4.1:+21965954 | None:intergenic |
| ATGGTAATGAGTATGACTCA+GGG | 0.675403 | 4.1:-21966007 | MS.gene26663:CDS |
| TGAACCACAGGCTTCCGTGG+CGG | 0.692610 | 4.1:+21966077 | None:intergenic |
| ATTACCCTTCAGAGCCGGTG+AGG | 0.718129 | 4.1:-21966204 | MS.gene26663:CDS |
| CATCCTGTGATCATCCACCG+GGG | 0.810049 | 4.1:+21966040 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATAATTATATTGTGTTT+TGG | - | chr4.1:21966113-21966132 | MS.gene26663:CDS | 15.0% |
| ! | AGTTCTTCAATTTCATCAAT+TGG | - | chr4.1:21966044-21966063 | MS.gene26663:CDS | 25.0% |
| ! | ATAGATTTGTCTGAGAAATT+GGG | - | chr4.1:21966194-21966213 | MS.gene26663:CDS | 25.0% |
| ! | GTTCTTCAATTTCATCAATT+GGG | - | chr4.1:21966045-21966064 | MS.gene26663:CDS | 25.0% |
| !! | TGGTTAATTTTGTGATGTTT+TGG | - | chr4.1:21966133-21966152 | MS.gene26663:CDS | 25.0% |
| GATAGATTTGTCTGAGAAAT+TGG | - | chr4.1:21966193-21966212 | MS.gene26663:CDS | 30.0% | |
| TAGATTTGTCTGAGAAATTG+GGG | - | chr4.1:21966195-21966214 | MS.gene26663:CDS | 30.0% | |
| !! | AAAAATTGCTGTTTCCAATG+AGG | - | chr4.1:21965948-21965967 | MS.gene26663:CDS | 30.0% |
| !! | AAAATTGCTGTTTCCAATGA+GGG | - | chr4.1:21965949-21965968 | MS.gene26663:CDS | 30.0% |
| !! | ACAAGCATAAGCTTTTTCTA+GGG | + | chr4.1:21966023-21966042 | None:intergenic | 30.0% |
| !! | TACAAGCATAAGCTTTTTCT+AGG | + | chr4.1:21966024-21966043 | None:intergenic | 30.0% |
| !!! | TGATGTTTTGGTTTGATTAG+TGG | - | chr4.1:21966145-21966164 | MS.gene26663:CDS | 30.0% |
| ! | ATGGTAATGAGTATGACTCA+GGG | - | chr4.1:21966368-21966387 | MS.gene26663:intron | 35.0% |
| !! | AAATTGCTGTTTCCAATGAG+GGG | - | chr4.1:21965950-21965969 | MS.gene26663:CDS | 35.0% |
| TAATGAGTATGACTCAGGGT+CGG | - | chr4.1:21966372-21966391 | MS.gene26663:intron | 40.0% | |
| TAGGAGGTTGAAGGATAAGA+AGG | - | chr4.1:21966253-21966272 | MS.gene26663:intron | 40.0% | |
| TCAGACAAATCTATCCTCAC+CGG | + | chr4.1:21966188-21966207 | None:intergenic | 40.0% | |
| ! | CATGGTAATGAGTATGACTC+AGG | - | chr4.1:21966367-21966386 | MS.gene26663:intron | 40.0% |
| !! | GGTGTTTTCATCTGTCGTTT+AGG | + | chr4.1:21965985-21966004 | None:intergenic | 40.0% |
| !!! | CTTTTTCTAGGGTTTCAAGC+TGG | + | chr4.1:21966012-21966031 | None:intergenic | 40.0% |
| AATGAGTATGACTCAGGGTC+GGG | - | chr4.1:21966373-21966392 | MS.gene26663:intron | 45.0% | |
| CTAGGGTTTCAAGCTGGAAA+CGG | + | chr4.1:21966006-21966025 | None:intergenic | 45.0% | |
| GGATAATTACCCTTCAGAGC+CGG | - | chr4.1:21966166-21966185 | MS.gene26663:CDS | 45.0% | |
| GTCTGAGAAATTGGGGTTGA+CGG | - | chr4.1:21966202-21966221 | MS.gene26663:CDS | 45.0% | |
| GTTCTGTCATAGGAGGTTGA+AGG | - | chr4.1:21966244-21966263 | MS.gene26663:intron | 45.0% | |
| ! | AGAAATTGGGGTTGACGGAT+CGG | - | chr4.1:21966207-21966226 | MS.gene26663:CDS | 45.0% |
| ! | AGATGTGGTTCTGTCATAGG+AGG | - | chr4.1:21966237-21966256 | MS.gene26663:intron | 45.0% |
| ! | TGCAGATGTGGTTCTGTCAT+AGG | - | chr4.1:21966234-21966253 | MS.gene26663:intron | 45.0% |
| GAATGCAATGGCACGAGTGT+TGG | - | chr4.1:21966426-21966445 | MS.gene26663:CDS | 50.0% | |
| AGGCTTACTCTGCCCCTCAT+TGG | + | chr4.1:21965965-21965984 | None:intergenic | 55.0% | |
| ATTACCCTTCAGAGCCGGTG+AGG | - | chr4.1:21966171-21966190 | MS.gene26663:CDS | 55.0% | |
| ATTCCCCGGTGGATGATCAC+AGG | - | chr4.1:21966332-21966351 | MS.gene26663:intron | 55.0% | |
| CACAGGATGGGGTTTGAGCA+TGG | - | chr4.1:21966349-21966368 | MS.gene26663:intron | 55.0% | |
| CATCCTGTGATCATCCACCG+GGG | + | chr4.1:21966338-21966357 | None:intergenic | 55.0% | |
| CCATCCTGTGATCATCCACC+GGG | + | chr4.1:21966339-21966358 | None:intergenic | 55.0% | |
| CCCATCCTGTGATCATCCAC+CGG | + | chr4.1:21966340-21966359 | None:intergenic | 55.0% | |
| CCGGTGGATGATCACAGGAT+GGG | - | chr4.1:21966337-21966356 | MS.gene26663:intron | 55.0% | |
| CGGTGGATGATCACAGGATG+GGG | - | chr4.1:21966338-21966357 | MS.gene26663:intron | 55.0% | |
| GAAGGAGTCTGCTCCCAAGA+AGG | - | chr4.1:21966271-21966290 | MS.gene26663:intron | 55.0% | |
| TATGACTCAGGGTCGGGTTC+AGG | - | chr4.1:21966379-21966398 | MS.gene26663:intron | 55.0% | |
| TGATCATCCACCGGGGAATC+CGG | + | chr4.1:21966331-21966350 | None:intergenic | 55.0% | |
| ! | ACTCGTGCCATTGCATTCCG+CGG | + | chr4.1:21966424-21966443 | None:intergenic | 55.0% |
| !! | CTATCCTCACCGGCTCTGAA+GGG | + | chr4.1:21966178-21966197 | None:intergenic | 55.0% |
| !! | TCTATCCTCACCGGCTCTGA+AGG | + | chr4.1:21966179-21966198 | None:intergenic | 55.0% |
| CCCGGTGGATGATCACAGGA+TGG | - | chr4.1:21966336-21966355 | MS.gene26663:intron | 60.0% | |
| CGGATCGGCAGTTGCAGATG+TGG | - | chr4.1:21966222-21966241 | MS.gene26663:intron | 60.0% | |
| GCCTGTGGTTCAGCCTTTGC+CGG | - | chr4.1:21966309-21966328 | MS.gene26663:intron | 60.0% | |
| GTTGGAGCCGCGGAATGCAA+TGG | - | chr4.1:21966414-21966433 | MS.gene26663:intron | 60.0% | |
| TCAGCCTTTGCCGGATTCCC+CGG | - | chr4.1:21966318-21966337 | MS.gene26663:intron | 60.0% | |
| TCCGGCAAAGGCTGAACCAC+AGG | + | chr4.1:21966313-21966332 | None:intergenic | 60.0% | |
| TGAACCACAGGCTTCCGTGG+CGG | + | chr4.1:21966301-21966320 | None:intergenic | 60.0% | |
| ! | GTCGGGTTCAGGTTCGAGTC+CGG | - | chr4.1:21966390-21966409 | MS.gene26663:intron | 60.0% |
| ! | TCGGGTTCAGGTTCGAGTCC+GGG | - | chr4.1:21966391-21966410 | MS.gene26663:intron | 60.0% |
| ! | TTCAGGTTCGAGTCCGGGGT+TGG | - | chr4.1:21966396-21966415 | MS.gene26663:intron | 60.0% |
| CCGTGGCGGCACACCTTCTT+GGG | + | chr4.1:21966287-21966306 | None:intergenic | 65.0% | |
| GGCTGAACCACAGGCTTCCG+TGG | + | chr4.1:21966304-21966323 | None:intergenic | 65.0% | |
| TCCACCGGGGAATCCGGCAA+AGG | + | chr4.1:21966325-21966344 | None:intergenic | 65.0% | |
| TCCGTGGCGGCACACCTTCT+TGG | + | chr4.1:21966288-21966307 | None:intergenic | 65.0% | |
| ! | CCCAAGAAGGTGTGCCGCCA+CGG | - | chr4.1:21966284-21966303 | MS.gene26663:intron | 65.0% |
| ! | CGGGTTCAGGTTCGAGTCCG+GGG | - | chr4.1:21966392-21966411 | MS.gene26663:intron | 65.0% |
| GCATTCCGCGGCTCCAACCC+CGG | + | chr4.1:21966412-21966431 | None:intergenic | 70.0% | |
| GCCTTTGCCGGATTCCCCGG+TGG | - | chr4.1:21966321-21966340 | MS.gene26663:intron | 70.0% | |
| TGTGCCGCCACGGAAGCCTG+TGG | - | chr4.1:21966294-21966313 | MS.gene26663:intron | 70.0% | |
| CGAGTCCGGGGTTGGAGCCG+CGG | - | chr4.1:21966404-21966423 | MS.gene26663:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 21965940 | 21966457 | 21965940 | ID=MS.gene26663 |
| chr4.1 | mRNA | 21965940 | 21966457 | 21965940 | ID=MS.gene26663.t1;Parent=MS.gene26663 |
| chr4.1 | exon | 21966421 | 21966457 | 21966421 | ID=MS.gene26663.t1.exon1;Parent=MS.gene26663.t1 |
| chr4.1 | CDS | 21966421 | 21966457 | 21966421 | ID=cds.MS.gene26663.t1;Parent=MS.gene26663.t1 |
| chr4.1 | exon | 21965940 | 21966232 | 21965940 | ID=MS.gene26663.t1.exon2;Parent=MS.gene26663.t1 |
| chr4.1 | CDS | 21965940 | 21966232 | 21965940 | ID=cds.MS.gene26663.t1;Parent=MS.gene26663.t1 |
| Gene Sequence |
| Protein sequence |
