Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26684.t1 | KEH32425.1 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 6.20E-30 | 140.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26684.t1 | A2XD79 | 47.0 | 83 | 41 | 2 | 32 | 111 | 96 | 178 | 3.6e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26684.t1 | A0A072V2U3 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 4.5e-30 | 140.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene26684.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26684.t1 | MTR_4g124950 | 70.175 | 114 | 22 | 2 | 1 | 106 | 1 | 110 | 8.13e-47 | 147 |
| MS.gene26684.t1 | MTR_4g124960 | 70.370 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 2.33e-45 | 145 |
| MS.gene26684.t1 | MTR_4g124960 | 70.755 | 106 | 30 | 1 | 1 | 106 | 67 | 171 | 1.70e-44 | 144 |
| MS.gene26684.t1 | MTR_2g042900 | 38.608 | 158 | 44 | 6 | 1 | 106 | 72 | 228 | 3.86e-22 | 87.8 |
| MS.gene26684.t1 | MTR_2g042900 | 43.617 | 94 | 33 | 3 | 1 | 74 | 72 | 165 | 3.15e-17 | 74.7 |
| MS.gene26684.t1 | MTR_5g013515 | 36.190 | 105 | 44 | 3 | 25 | 106 | 4 | 108 | 5.02e-14 | 64.3 |
| MS.gene26684.t1 | MTR_5g013530 | 40.625 | 96 | 40 | 3 | 30 | 108 | 17 | 112 | 1.22e-13 | 63.5 |
| MS.gene26684.t1 | MTR_5g013530 | 37.692 | 130 | 53 | 5 | 7 | 108 | 5 | 134 | 1.61e-13 | 63.5 |
| MS.gene26684.t1 | MTR_5g013520 | 37.234 | 94 | 42 | 2 | 30 | 106 | 10 | 103 | 7.89e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26684.t1 | AT1G19180 | 32.609 | 138 | 59 | 3 | 2 | 105 | 17 | 154 | 4.31e-11 | 57.4 |
| MS.gene26684.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
| MS.gene26684.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
Find 21 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTCACTTCTTCGATTCA+TGG | 0.237608 | 8.4:+9696307 | MS.gene26684:CDS |
| AATGTCAACTGTGCAGTTTC+CGG | 0.323565 | 8.4:-9696013 | None:intergenic |
| TCTAGTAACACAAGTGGCTT+TGG | 0.360249 | 8.4:-9696889 | None:intergenic |
| CAACCACCTCCTACACTAAC+TGG | 0.374106 | 8.4:+9696151 | MS.gene26684:CDS |
| TTATTTCTTTAAAGAAGATA+TGG | 0.376656 | 8.4:+9696271 | MS.gene26684:intron |
| TAATGGAGAAGTTATTGTAT+TGG | 0.400656 | 8.4:+9696042 | MS.gene26684:CDS |
| TAACTCAATGGTTAACAAAC+CGG | 0.425259 | 8.4:+9695994 | MS.gene26684:CDS |
| GGTGGTTGAAGACTCATATC+AGG | 0.439597 | 8.4:-9696136 | None:intergenic |
| AGCTCCTCAACTTTGTCAGC+AGG | 0.455494 | 8.4:-9696073 | None:intergenic |
| TTACAGAACTAATAACTCAA+TGG | 0.476118 | 8.4:+9695982 | MS.gene26684:intron |
| TTCTTCGATTCATGGAGAAA+AGG | 0.478429 | 8.4:+9696315 | MS.gene26684:CDS |
| CAATTACCAGTTAGTGTAGG+AGG | 0.522487 | 8.4:-9696157 | None:intergenic |
| CTGTGGATTCTTTGTCTCCA+AGG | 0.531713 | 8.4:+9695838 | MS.gene26684:CDS |
| TCATGGAGAAAAGGAAAGCC+AGG | 0.536399 | 8.4:+9696324 | MS.gene26684:CDS |
| CATTTGCAAGCACTCAAACT+CGG | 0.560353 | 8.4:+9696101 | MS.gene26684:CDS |
| CTCATATCAGGAATAACTGA+AGG | 0.598557 | 8.4:-9696124 | None:intergenic |
| TCAATTTCTAGTAACACAAG+TGG | 0.602083 | 8.4:-9696895 | None:intergenic |
| GTTTGTGGGAAATAAGTCCA+TGG | 0.603000 | 8.4:-9695793 | None:intergenic |
| TTACCAGTTAGTGTAGGAGG+TGG | 0.608915 | 8.4:-9696154 | None:intergenic |
| GAGCAATTACCAGTTAGTGT+AGG | 0.610011 | 8.4:-9696160 | None:intergenic |
| CTTGGAGACAAAGAATCCAC+AGG | 0.648213 | 8.4:-9695837 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATTTTGAAACAAATAA+AGG | + | chr8.4:9696195-9696214 | MS.gene26684:intron | 10.0% |
| !! | AAGAAATAAAATTTATGATC+AGG | - | chr8.4:9696260-9696279 | None:intergenic | 15.0% |
| !!! | AAGGAAATATTTTTTGTATT+AGG | + | chr8.4:9696214-9696233 | MS.gene26684:intron | 15.0% |
| !!! | AGGAAATATTTTTTGTATTA+GGG | + | chr8.4:9696215-9696234 | MS.gene26684:intron | 15.0% |
| !!! | ATTATGAATATTTTGATTAC+CGG | - | chr8.4:9696596-9696615 | None:intergenic | 15.0% |
| !!! | TAAATTCAAGTTTTTATTCA+AGG | + | chr8.4:9696365-9696384 | MS.gene26684:intron | 15.0% |
| !!! | TTATTTCTTTAAAGAAGATA+TGG | + | chr8.4:9696271-9696290 | MS.gene26684:intron | 15.0% |
| ! | TAATGGAGAAGTTATTGTAT+TGG | + | chr8.4:9696042-9696061 | MS.gene26684:CDS | 25.0% |
| ! | TTACAGAACTAATAACTCAA+TGG | + | chr8.4:9695982-9696001 | MS.gene26684:intron | 25.0% |
| ! | TTGTTTCAACATTTCAATCA+AGG | - | chr8.4:9696480-9696499 | None:intergenic | 25.0% |
| !!! | CAGTTGACATTGTTTTATAA+TGG | + | chr8.4:9696025-9696044 | MS.gene26684:CDS | 25.0% |
| !!! | GTAAAATGTGATTTTTGAGA+TGG | - | chr8.4:9696829-9696848 | None:intergenic | 25.0% |
| !!! | TTGAATTTAATTTGTCTGAG+AGG | - | chr8.4:9696354-9696373 | None:intergenic | 25.0% |
| GTTGTCGTAACTTTGTAATT+TGG | + | chr8.4:9696723-9696742 | MS.gene26684:intron | 30.0% | |
| TAACTCAATGGTTAACAAAC+CGG | + | chr8.4:9695994-9696013 | MS.gene26684:CDS | 30.0% | |
| !! | AGTAGTTGATGATATGCATA+AGG | - | chr8.4:9696796-9696815 | None:intergenic | 30.0% |
| !! | TCAATTTCTAGTAACACAAG+TGG | - | chr8.4:9696898-9696917 | None:intergenic | 30.0% |
| CTCATATCAGGAATAACTGA+AGG | - | chr8.4:9696127-9696146 | None:intergenic | 35.0% | |
| TAGAATTGGATGGAATCAGT+TGG | - | chr8.4:9696855-9696874 | None:intergenic | 35.0% | |
| TTCTTCGATTCATGGAGAAA+AGG | + | chr8.4:9696315-9696334 | MS.gene26684:CDS | 35.0% | |
| ! | AACTGATTCCATCCAATTCT+AGG | + | chr8.4:9696854-9696873 | MS.gene26684:intron | 35.0% |
| ! | AGAAGCACAAAAACATACCT+TGG | - | chr8.4:9695858-9695877 | None:intergenic | 35.0% |
| !!! | ACTTTTTTGCACACAAGCAA+TGG | - | chr8.4:9695921-9695940 | None:intergenic | 35.0% |
| !!! | CAACTTTCGTGTTGTTTTCA+CGG | - | chr8.4:9696707-9696726 | None:intergenic | 35.0% |
| !!! | CTTTTTTGCACACAAGCAAT+GGG | - | chr8.4:9695920-9695939 | None:intergenic | 35.0% |
| !!! | TTTTTTGCACACAAGCAATG+GGG | - | chr8.4:9695919-9695938 | None:intergenic | 35.0% |
| !!! | TTTTTTGGTGTCACGTTTGT+GGG | - | chr8.4:9695810-9695829 | None:intergenic | 35.0% |
| AATGTCAACTGTGCAGTTTC+CGG | - | chr8.4:9696016-9696035 | None:intergenic | 40.0% | |
| ACCAAAAAACGTTACACCTG+TGG | + | chr8.4:9695821-9695840 | MS.gene26684:CDS | 40.0% | |
| AGCTTCACTTCTTCGATTCA+TGG | + | chr8.4:9696307-9696326 | MS.gene26684:CDS | 40.0% | |
| ATAGACAGAGACATGGAGAA+GGG | - | chr8.4:9696457-9696476 | None:intergenic | 40.0% | |
| ATCAAGGATAGACAGAGACA+TGG | - | chr8.4:9696464-9696483 | None:intergenic | 40.0% | |
| CAAGCAAAATCAAGGACTTC+CGG | + | chr8.4:9696574-9696593 | MS.gene26684:intron | 40.0% | |
| CAATTACCAGTTAGTGTAGG+AGG | - | chr8.4:9696160-9696179 | None:intergenic | 40.0% | |
| CACTATTTATGTTCCTCTCG+TGG | - | chr8.4:9696642-9696661 | None:intergenic | 40.0% | |
| CATTTGCAAGCACTCAAACT+CGG | + | chr8.4:9696101-9696120 | MS.gene26684:CDS | 40.0% | |
| GAGCAATTACCAGTTAGTGT+AGG | - | chr8.4:9696163-9696182 | None:intergenic | 40.0% | |
| GCAATGGGGCAAAACAAAAA+TGG | - | chr8.4:9695905-9695924 | None:intergenic | 40.0% | |
| TCTAGTAACACAAGTGGCTT+TGG | - | chr8.4:9696892-9696911 | None:intergenic | 40.0% | |
| TTGTGCTCATGCTCTTACAA+GGG | - | chr8.4:9696668-9696687 | None:intergenic | 40.0% | |
| ! | TTTTCCTGCTGACAAAGTTG+AGG | + | chr8.4:9696069-9696088 | MS.gene26684:CDS | 40.0% |
| !!! | GTTTTTTGGTGTCACGTTTG+TGG | - | chr8.4:9695811-9695830 | None:intergenic | 40.0% |
| !!! | TCCACAGGTGTAACGTTTTT+TGG | - | chr8.4:9695825-9695844 | None:intergenic | 40.0% |
| !!! | TTCGTGTTGTTTTCACGGTA+CGG | - | chr8.4:9696702-9696721 | None:intergenic | 40.0% |
| CTCTTCAGCAAGCCTAGAAT+TGG | - | chr8.4:9696869-9696888 | None:intergenic | 45.0% | |
| CTGTGGATTCTTTGTCTCCA+AGG | + | chr8.4:9695838-9695857 | MS.gene26684:CDS | 45.0% | |
| CTTCGACGCAAGCAAAATCA+AGG | + | chr8.4:9696566-9696585 | MS.gene26684:intron | 45.0% | |
| CTTGGAGACAAAGAATCCAC+AGG | - | chr8.4:9695840-9695859 | None:intergenic | 45.0% | |
| GATAGACAGAGACATGGAGA+AGG | - | chr8.4:9696458-9696477 | None:intergenic | 45.0% | |
| GGTGGTTGAAGACTCATATC+AGG | - | chr8.4:9696139-9696158 | None:intergenic | 45.0% | |
| GTTGTGCTCATGCTCTTACA+AGG | - | chr8.4:9696669-9696688 | None:intergenic | 45.0% | |
| TCAGCAAGCCTAGAATTGGA+TGG | - | chr8.4:9696865-9696884 | None:intergenic | 45.0% | |
| TCATAGTCAAGAACCACGAG+AGG | + | chr8.4:9696626-9696645 | MS.gene26684:intron | 45.0% | |
| TCATGGAGAAAAGGAAAGCC+AGG | + | chr8.4:9696324-9696343 | MS.gene26684:CDS | 45.0% | |
| TTACCAGTTAGTGTAGGAGG+TGG | - | chr8.4:9696157-9696176 | None:intergenic | 45.0% | |
| ! | ATTTGTCTGAGAGGCATACC+TGG | - | chr8.4:9696345-9696364 | None:intergenic | 45.0% |
| ! | CTTGATTTTGCTTGCGTCGA+AGG | - | chr8.4:9696568-9696587 | None:intergenic | 45.0% |
| AGCTCCTCAACTTTGTCAGC+AGG | - | chr8.4:9696076-9696095 | None:intergenic | 50.0% | |
| CAACCACCTCCTACACTAAC+TGG | + | chr8.4:9696151-9696170 | MS.gene26684:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9695795 | 9696924 | 9695795 | ID=MS.gene26684 |
| chr8.4 | mRNA | 9695795 | 9696924 | 9695795 | ID=MS.gene26684.t1;Parent=MS.gene26684 |
| chr8.4 | exon | 9695795 | 9695859 | 9695795 | ID=MS.gene26684.t1.exon1;Parent=MS.gene26684.t1 |
| chr8.4 | CDS | 9695795 | 9695859 | 9695795 | ID=cds.MS.gene26684.t1;Parent=MS.gene26684.t1 |
| chr8.4 | exon | 9695988 | 9696172 | 9695988 | ID=MS.gene26684.t1.exon2;Parent=MS.gene26684.t1 |
| chr8.4 | CDS | 9695988 | 9696172 | 9695988 | ID=cds.MS.gene26684.t1;Parent=MS.gene26684.t1 |
| chr8.4 | exon | 9696285 | 9696345 | 9696285 | ID=MS.gene26684.t1.exon3;Parent=MS.gene26684.t1 |
| chr8.4 | CDS | 9696285 | 9696345 | 9696285 | ID=cds.MS.gene26684.t1;Parent=MS.gene26684.t1 |
| chr8.4 | exon | 9696876 | 9696924 | 9696876 | ID=MS.gene26684.t1.exon4;Parent=MS.gene26684.t1 |
| chr8.4 | CDS | 9696876 | 9696924 | 9696876 | ID=cds.MS.gene26684.t1;Parent=MS.gene26684.t1 |
| Gene Sequence |
| Protein sequence |
