AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27013


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27013.t1 MTR_1g023230 100.000 121 0 0 1 121 108 228 2.19e-85 254
MS.gene27013.t1 MTR_3g112510 61.538 221 68 9 11 226 82 290 2.20e-72 222
MS.gene27013.t1 MTR_8g076620 54.132 242 78 7 1 229 110 331 4.05e-68 213
MS.gene27013.t1 MTR_5g021130 54.661 236 90 6 7 229 92 323 7.09e-68 212
MS.gene27013.t1 MTR_5g089750 45.062 162 82 3 15 169 249 410 3.98e-38 138
MS.gene27013.t1 MTR_3g014660 40.331 181 90 5 1 168 119 294 1.06e-35 129
MS.gene27013.t1 MTR_4g071110 41.611 149 62 2 12 160 14 137 2.65e-34 124
MS.gene27013.t1 MTR_8g039110 39.779 181 96 3 15 182 124 304 1.53e-32 120
MS.gene27013.t1 MTR_0363s0040 75.556 45 11 0 15 59 124 168 2.25e-20 86.7
MS.gene27013.t1 MTR_0105s0070 63.636 33 12 0 17 49 44 76 9.73e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27013.t1 AT1G75520 52.340 235 87 7 11 229 121 346 1.42e-71 222
MS.gene27013.t1 AT3G51060 50.211 237 90 5 13 229 142 370 1.44e-68 215
MS.gene27013.t1 AT1G19790 50.000 244 105 7 1 228 102 344 1.03e-67 212
MS.gene27013.t1 AT1G19790 50.000 244 105 7 1 228 102 344 1.03e-67 212
MS.gene27013.t1 AT5G66350 49.333 225 91 6 15 229 120 331 6.76e-64 202
MS.gene27013.t1 AT5G66350 48.182 220 91 6 15 224 120 326 4.35e-61 194
MS.gene27013.t1 AT5G66350 49.065 214 86 6 15 218 120 320 6.38e-60 191
MS.gene27013.t1 AT2G21400 42.791 215 83 6 12 223 7 184 2.77e-48 157
MS.gene27013.t1 AT2G21400 42.791 215 83 6 12 223 6 183 2.81e-48 157
MS.gene27013.t1 AT4G36260 52.500 160 61 5 13 162 92 246 2.97e-46 156
MS.gene27013.t1 AT2G21400 41.981 212 74 6 12 223 6 168 3.52e-46 151
MS.gene27013.t1 AT2G21400 41.981 212 74 6 12 223 7 169 3.70e-46 151
MS.gene27013.t1 AT2G18120 47.134 157 56 3 13 162 70 206 1.92e-41 140
MS.gene27013.t1 AT2G18120 48.026 152 60 2 13 162 70 204 6.71e-39 134
MS.gene27013.t1 AT5G12330 45.625 160 75 3 15 162 112 271 1.57e-37 133
MS.gene27013.t1 AT5G12330 45.625 160 75 3 15 162 112 271 1.57e-37 133
MS.gene27013.t1 AT5G12330 45.625 160 75 3 15 162 199 358 1.75e-37 135
MS.gene27013.t1 AT5G33210 60.870 92 32 2 13 104 15 102 2.25e-31 111
MS.gene27013.t1 AT5G33210 48.819 127 54 4 13 139 50 165 3.18e-31 113
MS.gene27013.t1 AT3G54430 35.185 162 81 4 11 168 37 178 1.09e-23 94.0
MS.gene27013.t1 AT5G12330 82.609 46 8 0 15 60 112 157 9.83e-22 90.1

Find 59 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGATCCCTCTGGAGCTTC+TGG 0.278538 1.3:+2140833 MS.gene27013:CDS
AGCTCCTCTTAATGCTTTCA+TGG 0.295162 1.3:+2141700 MS.gene27013:CDS
CACTCATGTCTTTAGGATCT+TGG 0.300540 1.3:-2141746 None:intergenic
TGAATTCACCGGCGGTGTTC+AGG 0.312818 1.3:+2141420 MS.gene27013:CDS
TATATACCTGTGGTAGTAAT+AGG 0.332609 1.3:-2140878 None:intergenic
AGGTCTTCAAGTGGTGGTTT+AGG 0.350386 1.3:+2140575 MS.gene27013:CDS
GGTGGTGGCGAGGGTTCTTC+CGG 0.367254 1.3:+2141584 MS.gene27013:CDS
CCACCACCAGCAGCAGCATT+TGG 0.392576 1.3:-2141569 None:intergenic
CGTTCGCGGCGTTTAGCAGC+TGG 0.397352 1.3:-2140725 None:intergenic
CCTCTTAATGCTTTCATGGC+TGG 0.408461 1.3:+2141704 MS.gene27013:CDS
GTGGACAATCTTTCTTAGCT+TGG 0.415642 1.3:-2140631 None:intergenic
TGAGTTGTTGCTGCCGTTCG+CGG 0.429437 1.3:-2140739 None:intergenic
GGCGAGGGTTCTTCCGGTGG+TGG 0.430350 1.3:+2141590 MS.gene27013:CDS
TTGAAATTAGGGTTGGAGTT+GGG 0.437119 1.3:+2141380 MS.gene27013:intron
AGTGGTGGTTTAGGTGGTGG+TGG 0.442865 1.3:+2140584 MS.gene27013:CDS
GGTTCTTCCGGTGGTGGCAG+CGG 0.446891 1.3:+2141596 MS.gene27013:CDS
TTCGCCACCGCTGCCACCAC+CGG 0.449406 1.3:-2141603 None:intergenic
CCAAATGCTGCTGCTGGTGG+TGG 0.457278 1.3:+2141569 MS.gene27013:CDS
AAACTCATGTGAAAAGTACT+TGG 0.464467 1.3:+2140699 MS.gene27013:CDS
TGCTGCTGCTGGTGGTGGCG+AGG 0.465038 1.3:+2141574 MS.gene27013:CDS
TTGTATGATCAAGGACTTGA+AGG 0.474373 1.3:+2141533 MS.gene27013:CDS
GAAAATCAAAATGATCCCTC+TGG 0.474468 1.3:+2140824 MS.gene27013:CDS
TGTCTTTAGGATCTTGGAGG+TGG 0.488834 1.3:-2141740 None:intergenic
GCTGCTGCTGGTGGTGGCGA+GGG 0.489260 1.3:+2141575 MS.gene27013:CDS
TCATGTCTTTAGGATCTTGG+AGG 0.489282 1.3:-2141743 None:intergenic
TATAGTGAAGAATACTCAAA+TGG 0.492700 1.3:-2141674 None:intergenic
TCAAGTGGTGGTTTAGGTGG+TGG 0.495997 1.3:+2140581 MS.gene27013:CDS
ATGAAAGCATTAAGAGGAGC+TGG 0.499046 1.3:-2141698 None:intergenic
CCTGTGGTAGTAATAGGTAC+TGG 0.504192 1.3:-2140872 None:intergenic
TCTTCCGGTGGTGGCAGCGG+TGG 0.515802 1.3:+2141599 MS.gene27013:CDS
TCTTCAAGTGGTGGTTTAGG+TGG 0.516785 1.3:+2140578 MS.gene27013:CDS
AGTAATAGGTACTGGAGTAC+AGG 0.518362 1.3:-2140864 None:intergenic
CCAGCCATGAAAGCATTAAG+AGG 0.523927 1.3:-2141704 None:intergenic
TTTGAAATTAGGGTTGGAGT+TGG 0.534737 1.3:+2141379 MS.gene27013:intron
GGTGGTGGCAGCGGTGGCGA+AGG 0.539736 1.3:+2141605 MS.gene27013:CDS
TTTACACACCTGAACACCGC+CGG 0.552531 1.3:-2141428 None:intergenic
TCCACCGGAGCTGAATTCAC+CGG 0.555263 1.3:+2141409 MS.gene27013:CDS
GAACTTGTTGCAAGAGTCGA+GGG 0.557726 1.3:+2140666 MS.gene27013:CDS
CAGCTGCTAAACGCCGCGAA+CGG 0.557914 1.3:+2140726 MS.gene27013:CDS
ATTGTTAACATTTGTTGTCG+TGG 0.557983 1.3:-2141651 None:intergenic
GTCTTTAGGATCTTGGAGGT+GGG 0.561171 1.3:-2141739 None:intergenic
CCAGTACCTATTACTACCAC+AGG 0.564967 1.3:+2140872 MS.gene27013:CDS
CAAACTGCTGTGAACATAGG+AGG 0.565014 1.3:+2141491 MS.gene27013:CDS
ACTAGTCCAAATGCTGCTGC+TGG 0.565581 1.3:+2141563 MS.gene27013:CDS
GCCGGTGAATTCAGCTCCGG+TGG 0.569608 1.3:-2141410 None:intergenic
TATCAAACTGCTGTGAACAT+AGG 0.572121 1.3:+2141488 MS.gene27013:CDS
GGTGGCGAGGGTTCTTCCGG+TGG 0.572206 1.3:+2141587 MS.gene27013:CDS
ACCGCCGGTGAATTCAGCTC+CGG 0.574562 1.3:-2141413 None:intergenic
TAGAGAACCAGAAGCTCCAG+AGG 0.578477 1.3:-2140840 None:intergenic
AGTCCAAATGCTGCTGCTGG+TGG 0.584415 1.3:+2141566 MS.gene27013:CDS
AACTCATGTGAAAAGTACTT+GGG 0.591492 1.3:+2140700 MS.gene27013:CDS
AGAACTTGTTGCAAGAGTCG+AGG 0.603647 1.3:+2140665 MS.gene27013:CDS
GTGATGAGGTCTTCAAGTGG+TGG 0.621009 1.3:+2140569 None:intergenic
CAAGTTCTACATCTAAGATG+TGG 0.632750 1.3:-2140650 None:intergenic
GGAATGAACTGTCAAGATTG+TGG 0.658447 1.3:+2140605 MS.gene27013:CDS
ACCGGAGCTGAATTCACCGG+CGG 0.668533 1.3:+2141412 MS.gene27013:CDS
AGAGAACCAGAAGCTCCAGA+GGG 0.671479 1.3:-2140839 None:intergenic
GTGTGTAAAAGTGAGTGCAA+TGG 0.678715 1.3:+2141442 MS.gene27013:CDS
ACAGTGATGAGGTCTTCAAG+TGG 0.705760 1.3:+2140566 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTGATTTGAAATTA+GGG + chr1.3:2141369-2141388 MS.gene27013:intron 10.0%
!!! TTTTTATTTGATTTGAAATT+AGG + chr1.3:2141368-2141387 MS.gene27013:intron 10.0%
!! ATAGAATATATAGACTAATT+AGG - chr1.3:2141154-2141173 None:intergenic 15.0%
!!! TCTTTTTGAATTTTTTGATT+TGG + chr1.3:2141334-2141353 MS.gene27013:intron 15.0%
!! AAAATGAAAAGATATTACGA+GGG + chr1.3:2141001-2141020 MS.gene27013:intron 20.0%
!! TAAAATGAAAAGATATTACG+AGG + chr1.3:2141000-2141019 MS.gene27013:intron 20.0%
!! TCTATGAATCTATGATAAAT+AGG + chr1.3:2141125-2141144 MS.gene27013:intron 20.0%
!!! TAGTTTTTTGATATATAGCT+AGG + chr1.3:2141040-2141059 MS.gene27013:intron 20.0%
! TAGCTATATAGAAACAATTG+AGG + chr1.3:2141307-2141326 MS.gene27013:intron 25.0%
! TATAGTGAAGAATACTCAAA+TGG - chr1.3:2141677-2141696 None:intergenic 25.0%
! TGAAAAAATTGTTAGGTAAG+TGG + chr1.3:2141255-2141274 MS.gene27013:intron 25.0%
! TGAAGGATGAAAAAATTGTT+AGG + chr1.3:2141248-2141267 MS.gene27013:intron 25.0%
! TTTCTACATATATATACCTG+TGG - chr1.3:2140891-2140910 None:intergenic 25.0%
!!! ATTTGATTTGAAATTAGGGT+TGG + chr1.3:2141373-2141392 MS.gene27013:intron 25.0%
ATCCTTCAGAAGAAAAGAAA+GGG - chr1.3:2141236-2141255 None:intergenic 30.0%
TATATACCTGTGGTAGTAAT+AGG - chr1.3:2140881-2140900 None:intergenic 30.0%
TTGATAGAGTATGAAAGGAA+TGG - chr1.3:2141181-2141200 None:intergenic 30.0%
! AAGGGAATTTTGTATGATCA+AGG + chr1.3:2141524-2141543 MS.gene27013:CDS 30.0%
!! AAACTCATGTGAAAAGTACT+TGG + chr1.3:2140699-2140718 MS.gene27013:CDS 30.0%
!! AACTCATGTGAAAAGTACTT+GGG + chr1.3:2140700-2140719 MS.gene27013:CDS 30.0%
!! ATTGTTAACATTTGTTGTCG+TGG - chr1.3:2141654-2141673 None:intergenic 30.0%
!! CTAAGTTGATAGAGTATGAA+AGG - chr1.3:2141186-2141205 None:intergenic 30.0%
CATCCTTCAGAAGAAAAGAA+AGG - chr1.3:2141237-2141256 None:intergenic 35.0%
GAAAATCAAAATGATCCCTC+TGG + chr1.3:2140824-2140843 MS.gene27013:CDS 35.0%
TATCAAACTGCTGTGAACAT+AGG + chr1.3:2141488-2141507 MS.gene27013:CDS 35.0%
TTGAAATTAGGGTTGGAGTT+GGG + chr1.3:2141380-2141399 MS.gene27013:intron 35.0%
TTGTATGATCAAGGACTTGA+AGG + chr1.3:2141533-2141552 MS.gene27013:CDS 35.0%
! ATAGGAGGAAGAGTTTTCAA+GGG + chr1.3:2141506-2141525 MS.gene27013:CDS 35.0%
! CAAGTTCTACATCTAAGATG+TGG - chr1.3:2140653-2140672 None:intergenic 35.0%
! TTCCCTTTCTTTTCTTCTGA+AGG + chr1.3:2141231-2141250 MS.gene27013:intron 35.0%
! TTTGAAATTAGGGTTGGAGT+TGG + chr1.3:2141379-2141398 MS.gene27013:intron 35.0%
AGCTCCTCTTAATGCTTTCA+TGG + chr1.3:2141700-2141719 MS.gene27013:CDS 40.0%
GGAATGAACTGTCAAGATTG+TGG + chr1.3:2140605-2140624 MS.gene27013:CDS 40.0%
GTGGACAATCTTTCTTAGCT+TGG - chr1.3:2140634-2140653 None:intergenic 40.0%
GTGTGTAAAAGTGAGTGCAA+TGG + chr1.3:2141442-2141461 MS.gene27013:CDS 40.0%
! CATAGGAGGAAGAGTTTTCA+AGG + chr1.3:2141505-2141524 MS.gene27013:CDS 40.0%
!! AGTAATAGGTACTGGAGTAC+AGG - chr1.3:2140867-2140886 None:intergenic 40.0%
!! ATGAAAGCATTAAGAGGAGC+TGG - chr1.3:2141701-2141720 None:intergenic 40.0%
AGGTCTTCAAGTGGTGGTTT+AGG + chr1.3:2140575-2140594 MS.gene27013:CDS 45.0%
CAAACTGCTGTGAACATAGG+AGG + chr1.3:2141491-2141510 MS.gene27013:CDS 45.0%
CCAGTACCTATTACTACCAC+AGG + chr1.3:2140872-2140891 MS.gene27013:CDS 45.0%
CCTCTTAATGCTTTCATGGC+TGG + chr1.3:2141704-2141723 MS.gene27013:CDS 45.0%
CCTGTGGTAGTAATAGGTAC+TGG - chr1.3:2140875-2140894 None:intergenic 45.0%
GCTTAGTGATAGAGATCTCG+AGG + chr1.3:2141089-2141108 MS.gene27013:intron 45.0%
TCTTCAAGTGGTGGTTTAGG+TGG + chr1.3:2140578-2140597 MS.gene27013:CDS 45.0%
! GGAGTTGGGAAATTTTCCAC+CGG + chr1.3:2141394-2141413 MS.gene27013:CDS 45.0%
!! AGAACTTGTTGCAAGAGTCG+AGG + chr1.3:2140665-2140684 MS.gene27013:CDS 45.0%
!! CCAGCCATGAAAGCATTAAG+AGG - chr1.3:2141707-2141726 None:intergenic 45.0%
!! GAACTTGTTGCAAGAGTCGA+GGG + chr1.3:2140666-2140685 MS.gene27013:CDS 45.0%
ACTAGTCCAAATGCTGCTGC+TGG + chr1.3:2141563-2141582 MS.gene27013:CDS 50.0%
AGAGAACCAGAAGCTCCAGA+GGG - chr1.3:2140842-2140861 None:intergenic 50.0%
TAGAGAACCAGAAGCTCCAG+AGG - chr1.3:2140843-2140862 None:intergenic 50.0%
TCAAGTGGTGGTTTAGGTGG+TGG + chr1.3:2140581-2140600 MS.gene27013:CDS 50.0%
TTTACACACCTGAACACCGC+CGG - chr1.3:2141431-2141450 None:intergenic 50.0%
!! AATGATCCCTCTGGAGCTTC+TGG + chr1.3:2140833-2140852 MS.gene27013:CDS 50.0%
AGTCCAAATGCTGCTGCTGG+TGG + chr1.3:2141566-2141585 MS.gene27013:CDS 55.0%
TCCACCGGAGCTGAATTCAC+CGG + chr1.3:2141409-2141428 MS.gene27013:CDS 55.0%
TGAATTCACCGGCGGTGTTC+AGG + chr1.3:2141420-2141439 MS.gene27013:CDS 55.0%
TGAGTTGTTGCTGCCGTTCG+CGG - chr1.3:2140742-2140761 None:intergenic 55.0%
! AGTGGTGGTTTAGGTGGTGG+TGG + chr1.3:2140584-2140603 MS.gene27013:CDS 55.0%
ACCGCCGGTGAATTCAGCTC+CGG - chr1.3:2141416-2141435 None:intergenic 60.0%
ACCGGAGCTGAATTCACCGG+CGG + chr1.3:2141412-2141431 MS.gene27013:CDS 60.0%
CAGCTGCTAAACGCCGCGAA+CGG + chr1.3:2140726-2140745 MS.gene27013:CDS 60.0%
CCAAATGCTGCTGCTGGTGG+TGG + chr1.3:2141569-2141588 MS.gene27013:CDS 60.0%
CCACCACCAGCAGCAGCATT+TGG - chr1.3:2141572-2141591 None:intergenic 60.0%
CGTTCGCGGCGTTTAGCAGC+TGG - chr1.3:2140728-2140747 None:intergenic 65.0%
GCCGGTGAATTCAGCTCCGG+TGG - chr1.3:2141413-2141432 None:intergenic 65.0%
! GGTGGTGGCGAGGGTTCTTC+CGG + chr1.3:2141584-2141603 MS.gene27013:CDS 65.0%
! GGTTCTTCCGGTGGTGGCAG+CGG + chr1.3:2141596-2141615 MS.gene27013:CDS 65.0%
GGCGAGGGTTCTTCCGGTGG+TGG + chr1.3:2141590-2141609 MS.gene27013:CDS 70.0%
GGTGGCGAGGGTTCTTCCGG+TGG + chr1.3:2141587-2141606 MS.gene27013:CDS 70.0%
TTCGCCACCGCTGCCACCAC+CGG - chr1.3:2141606-2141625 None:intergenic 70.0%
! TCTTCCGGTGGTGGCAGCGG+TGG + chr1.3:2141599-2141618 MS.gene27013:CDS 70.0%
!! GCTGCTGCTGGTGGTGGCGA+GGG + chr1.3:2141575-2141594 MS.gene27013:CDS 70.0%
!! TGCTGCTGCTGGTGGTGGCG+AGG + chr1.3:2141574-2141593 MS.gene27013:CDS 70.0%
! GGTGGTGGCAGCGGTGGCGA+AGG + chr1.3:2141605-2141624 MS.gene27013:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 2140572 2141757 2140572 ID=MS.gene27013
chr1.3 mRNA 2140572 2141757 2140572 ID=MS.gene27013.t1;Parent=MS.gene27013
chr1.3 exon 2140572 2140893 2140572 ID=MS.gene27013.t1.exon1;Parent=MS.gene27013.t1
chr1.3 CDS 2140572 2140893 2140572 ID=cds.MS.gene27013.t1;Parent=MS.gene27013.t1
chr1.3 exon 2141390 2141757 2141390 ID=MS.gene27013.t1.exon2;Parent=MS.gene27013.t1
chr1.3 CDS 2141390 2141757 2141390 ID=cds.MS.gene27013.t1;Parent=MS.gene27013.t1
Gene Sequence

>MS.gene27013

ATGAGGTCTTCAAGTGGTGGTTTAGGTGGTGGTGGAATGAACTGTCAAGATTGTGGTAACCAAGCTAAGAAAGATTGTCCACATCTTAGATGTAGAACTTGTTGCAAGAGTCGAGGGTTTCAGTGTCAAACTCATGTGAAAAGTACTTGGGTTCCAGCTGCTAAACGCCGCGAACGGCAGCAACAACTCACGTCGTTGCAGCAACAAAATCAGCAGCAGCAACAACAGTTTCACTCATCAAGAAGACACAGAGAAAATCAAAATGATCCCTCTGGAGCTTCTGGTTCTCTAACCTGTACTCCAGTACCTATTACTACCACAGGTATATATATGTAGAAATATAAACTTTTGAACGATATACATCAACTTTCTTTGACTCACTTGTGTTTTTATTTCTTTACAGAAGAGTCGTTGAAAGAGAGAGTATATAAAATGAAAAGATATTACGAGGGTTTGAATTATAGTGTTTAGTTTTTTGATATATAGCTAGGTCAGTGACGAATTTTCAAACTATATAGCTTAGTGATAGAGATCTCGAGGAGAAACTATAGTTTCTATGAATCTATGATAAATAGGATACCTAATTAGTCTATATATTCTATATATCCATTCCTTTCATACTCTATCAACTTAGTTTTTTTCTTTTCTCTCTCTCTTTTTTCCCTTTCTTTTCTTCTGAAGGATGAAAAAATTGTTAGGTAAGTGGTTCTTTCTTTCTTTTAAATTTGAAAATGATAGCTATATAGAAACAATTGAGGTTTCTCTTTTTGAATTTTTTGATTTGGATTTAATTTTTTTTTTATTTGATTTGAAATTAGGGTTGGAGTTGGGAAATTTTCCACCGGAGCTGAATTCACCGGCGGTGTTCAGGTGTGTAAAAGTGAGTGCAATGGATGCACAAGATGAACGTTATGCTTATCAAACTGCTGTGAACATAGGAGGAAGAGTTTTCAAGGGAATTTTGTATGATCAAGGACTTGAAGGTAGCTACACTAGTCCAAATGCTGCTGCTGGTGGTGGCGAGGGTTCTTCCGGTGGTGGCAGCGGTGGCGAAGGAAATCAACAAAATCTCATAACAACCACGACAACAAATGTTAACAATCCATTTGAGTATTCTTCACTATATCCAGCTCCTCTTAATGCTTTCATGGCTGGTACGCAATTCTTCCCACCTCCAAGATCCTAA

Protein sequence

>MS.gene27013.t1

MRSSSGGLGGGGMNCQDCGNQAKKDCPHLRCRTCCKSRGFQCQTHVKSTWVPAAKRRERQQQLTSLQQQNQQQQQQFHSSRRHRENQNDPSGASGSLTCTPVPITTTGLELGNFPPELNSPAVFRCVKVSAMDAQDERYAYQTAVNIGGRVFKGILYDQGLEGSYTSPNAAAGGGEGSSGGGSGGEGNQQNLITTTTTNVNNPFEYSSLYPAPLNAFMAGTQFFPPPRS