AlfalfaGEDB | MS.gene27180

M. sativa cultivar XinJiangDaYe / MS.gene27180

KEGG: K13425
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27180.t1	KEH36934.1	65.3	556	107	3	1	556	1	470	1.90E-169	605.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27180.t1	Q9FLX8	52.0	98	45	2	315	412	144	239	5.9e-23	110.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27180.t1	A0A072V4M0	65.3	556	107	3	1	556	1	470	1.3e-169	605.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene27180.t1	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27180.t1	MTR_2g024380	87.179	312	40	0	245	556	159	470	0.0	574
MS.gene27180.t1	MTR_2g024380	74.468	235	50	2	1	235	1	225	1.28e-88	281
MS.gene27180.t1	MTR_2g024350	87.665	227	28	0	330	556	1	227	5.65e-146	419
MS.gene27180.t1	MTR_6g053200	39.812	319	160	6	255	556	233	536	8.75e-67	226
MS.gene27180.t1	MTR_6g053200	48.958	96	43	1	1	90	1	96	1.14e-14	77.0
MS.gene27180.t1	MTR_7g104980	51.759	199	95	1	358	556	18	215	5.19e-55	185
MS.gene27180.t1	MTR_8g067635	35.925	373	162	7	171	469	135	504	8.31e-54	191
MS.gene27180.t1	MTR_8g067650	36.228	334	142	6	253	555	226	519	4.67e-53	189
MS.gene27180.t1	MTR_8g069935	41.294	201	87	2	256	426	220	419	2.95e-40	152
MS.gene27180.t1	MTR_1g099600	53.061	98	44	2	315	412	164	259	2.78e-29	118

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27180.t1	AT4G01250	48.454	97	49	1	315	411	106	201	3.02e-26	109
MS.gene27180.t1	AT5G52830	52.041	98	45	2	315	412	144	239	3.05e-26	110
MS.gene27180.t1	AT3G04670	48.936	94	38	1	313	396	228	321	4.56e-24	103
MS.gene27180.t1	AT5G28650	47.872	94	39	1	313	396	228	321	8.70e-24	102
MS.gene27180.t1	AT4G31550	47.917	96	43	2	313	403	218	311	4.74e-23	100
MS.gene27180.t1	AT2G24570	58.571	70	29	0	334	403	240	309	5.36e-23	100
MS.gene27180.t1	AT4G31550	57.143	70	30	0	334	403	243	312	9.80e-23	99.8
MS.gene27180.t1	AT4G23550	47.253	91	47	1	321	411	124	213	3.07e-22	97.8
MS.gene27180.t1	AT2G23320	52.326	86	34	2	315	395	215	298	3.50e-22	97.8
MS.gene27180.t1	AT4G24240	49.495	99	43	2	302	395	243	339	6.20e-22	97.8

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Find 155 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGGTGTGACACTTAGA+GGG	0.602585	2.1:+64845365	None:intergenic
AAATCCAGGTTCCCCTAGTA+TGG	0.366946	2.1:-64843802	MS.gene27180:CDS
AACCTCCCTTATTCTGAAAC+TGG	0.323380	2.1:-64843558	MS.gene27180:CDS
AATTTGATGAACCACAAAGT+AGG	0.604187	2.1:-64843608	MS.gene27180:CDS
ACAACATTCTTATTAAATGT+TGG	0.443200	2.1:+64843903	None:intergenic
ACATAATAACCAAATGGCAT+TGG	0.531292	2.1:-64844411	MS.gene27180:CDS
ACCCAAATTTCTAGCGTTTA+AGG	0.162127	2.1:+64843653	None:intergenic
ACCTGGGTATTGCTGCTCTC+CGG	0.486470	2.1:+64843759	None:intergenic
ACGTGCATACAACCATCCAT+CGG	0.691580	2.1:-64844591	MS.gene27180:CDS
ACTTATATTGTAACATATGA+AGG	0.520110	2.1:-64843945	MS.gene27180:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTGATGAACCACAAAGT+AGG	-	chr2.1:64845331-64845350	MS.gene27180:CDS	30.0%
ACATAATAACCAAATGGCAT+TGG	-	chr2.1:64844528-64844547	MS.gene27180:intron	30.0%
AGTGTTAATGTTAGTGATCT+GGG	+	chr2.1:64843816-64843835	None:intergenic	30.0%
ATCCTTAAACGCTAGAAATT+TGG	-	chr2.1:64845284-64845303	MS.gene27180:CDS	30.0%
ATGAACACTAGTGTTTATCA+GGG	+	chr2.1:64843966-64843985	None:intergenic	30.0%
ATGAATCCAGTTTCAGAATA+AGG	+	chr2.1:64845390-64845409	None:intergenic	30.0%
GTAAAATCATCATAGCAAAG+TGG	+	chr2.1:64843593-64843612	None:intergenic	30.0%
TAGTGTTAATGTTAGTGATC+TGG	+	chr2.1:64843817-64843836	None:intergenic	30.0%
TCCTTAAACGCTAGAAATTT+GGG	-	chr2.1:64845285-64845304	MS.gene27180:CDS	30.0%
TGAATCCAGTTTCAGAATAA+GGG	+	chr2.1:64845389-64845408	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	64843443	64845518	64843443	ID=MS.gene27180
chr2.1	mRNA	64843443	64845518	64843443	ID=MS.gene27180.t1;Parent=MS.gene27180
chr2.1	exon	64844566	64845518	64844566	ID=MS.gene27180.t1.exon1;Parent=MS.gene27180.t1
chr2.1	CDS	64844566	64845518	64844566	ID=cds.MS.gene27180.t1;Parent=MS.gene27180.t1
chr2.1	exon	64844320	64844436	64844320	ID=MS.gene27180.t1.exon2;Parent=MS.gene27180.t1
chr2.1	CDS	64844320	64844436	64844320	ID=cds.MS.gene27180.t1;Parent=MS.gene27180.t1
chr2.1	exon	64843443	64844043	64843443	ID=MS.gene27180.t1.exon3;Parent=MS.gene27180.t1
chr2.1	CDS	64843443	64844043	64843443	ID=cds.MS.gene27180.t1;Parent=MS.gene27180.t1

Gene Sequence

>MS.gene27180

ATGGATAATGATGATCGAGATCTCTTTTCTATTGTTCGTAGTTGCAACGCTACCACATTTACTGGTCCTACCAACATTTTTGAAAATCCACCGCCACCTCAAATCGAAACCACTACCAATAACATCACATTCCCTCTAAGTGTCACACCACTTTGCTATGATGATTTTACTTTTAACCAAGAAAATAGTTCAGTCTCTTTTGCCCCACTTAAACCAAATGACTTCATAAATCTAGATAAATTGATGATCAATTTCAATCCCATCCCCACCTCAGCTCCCACTAGTATGCCAAAAATCAATACCACCACCACATCTTCCCCCGCCACCACCACCTACACTATCCCACCCCCCTTGACCACCACCATCCCCACCCAGATCACTAACATTAACACTAGTGTTCATATTATCAACACTCCCACCATCACTATTCCCACCACTCCCCCTACCATTACCCCCATTATCACTGCCACTGTAATCACCCCGACCACCACTACCCCTATCATATCTGCTACAAGCACTACCCTGATAAACACTAGTGTTCATGAAACCAATCAATATCCCGCCAATTATAATTTTCCAATACCCGCTGGACAACAACAAATTCAACAAAACATCAACAATCAAGTGTCTGTTCCCAAACATGTTGCATGCATTGATATCACAGCCACCCATTTTGATCGTGCATCCAACCATCCATCAGTTATCGCCTCGACCACCACTACCCCAATCATATCTGCTAAGAGCACTACGATGACAAACACCAGTGTTCATGAAACCAATCAATATTCCACAAATTATAATTTCCCAATGCACGCTGGACAAAAACAGATTCAACAAAACATCAACAATCAAGTGTATGTTCCCAAACATGTTGCATGCATTGATATCACAACCACTCATTTTGAACGTGCATACAACCATCCATCGGTTGCACTATGCGGATCAAGAAAAAGGTAATGTATTTATTCTATTATTTCATATATTTTAATTATAAATCAATTTTATTATGTCAAAATAATTGAAATATTTGACGTAACTTAGTTCAAGTGAACTAAAATTATTTTACCTTTCTTTTCTTATAGAAAACATAATAACCAAATGGCATTGGTGTGCTATATGAGGGACGATGAGCTTCCAAAAGACCCGTGGGCATGGAGGAAATATGGGCAAAAACCCATTAAAGGGTCTCCACATCCGAGGTTTACAAATATTCAAAACTTTATCTTTAACATATTTCTCTTTTCTTCCACATTATAAACAATAATTTTGTTACCTAAAACAAACACTCGCACCCTCGTGTTTTAAACAGTGATGGTAAGCATTTAGATCCTTGTCAGTGCATATAATTGTAATTAAATTCAACATTTGTTATCTTAATCATAGTTTCGTTTGTGTTTCAATTTTTAAAACATCAAAAACTGGTGTATATTTAGTCCATAAATGATGTTGAAAGAAATCAATAAATGATGTTGCAGGAGCTATTACAAATGTAGCACTTGGTCTGATTGTCCAGCAAGAAGAAAGGTTGAAAAATGCAAAACTCAAGAGAACACTTATATTGTAACATATGAAGGAGAGCACAATCATGCAAAGCCAACATTTAATAAGAATGTTGTTGTTGGTACATCCCAGCGCAAGTCACTGGAGACTGGGTTACACTCTGCGGAAGAAACTGGATCATCTACAAACATCACAAATCCAGGTTCCCCTAGTATGGTGATGTTGCAGATCGATCATCCGGAGAGCAGCAATACCCAGGTTCTCGCTGATCAATCAAAAATCCTTTATCCAGAAATAGAACTATTAGAGAGTCACTTACCCTCTGTGGAAGAAGTTGGATCATCCTTAAACGCTAGAAATTTGGGTTCACATAACAAAATGATTATGCAATTTGATGAACCACAAAGTAGGAATGTTACAGGTGTTGTCGATGACTCAAACCTCCCTTATTCTGAAACTGGATTCATTGGAAGCTTTGATGATAATGATGACATTTTGATACCAAACATAACAGCTTGGTCAGAAGATTATTTAATGGACTTCAAGTGCCTCAATGGTGGCGCTATATTCCCTTAG

Protein sequence

>MS.gene27180.t1

MDNDDRDLFSIVRSCNATTFTGPTNIFENPPPPQIETTTNNITFPLSVTPLCYDDFTFNQENSSVSFAPLKPNDFINLDKLMINFNPIPTSAPTSMPKINTTTTSSPATTTYTIPPPLTTTIPTQITNINTSVHIINTPTITIPTTPPTITPIITATVITPTTTTPIISATSTTLINTSVHETNQYPANYNFPIPAGQQQIQQNINNQVSVPKHVACIDITATHFDRASNHPSVIASTTTTPIISAKSTTMTNTSVHETNQYSTNYNFPMHAGQKQIQQNINNQVYVPKHVACIDITTTHFERAYNHPSVALCGSRKRKHNNQMALVCYMRDDELPKDPWAWRKYGQKPIKGSPHPRSYYKCSTWSDCPARRKVEKCKTQENTYIVTYEGEHNHAKPTFNKNVVVGTSQRKSLETGLHSAEETGSSTNITNPGSPSMVMLQIDHPESSNTQVLADQSKILYPEIELLESHLPSVEEVGSSLNARNLGSHNKMIMQFDEPQSRNVTGVVDDSNLPYSETGFIGSFDDNDDILIPNITAWSEDYLMDFKCLNGGAIFP