Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27404.t1 | XP_013441754.1 | 87.8 | 296 | 35 | 1 | 1 | 295 | 1 | 296 | 2.40E-147 | 531.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27404.t1 | Q9LKR4 | 23.8 | 260 | 168 | 6 | 40 | 287 | 60 | 301 | 1.0e-13 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27404.t1 | A0A072TE13 | 87.8 | 296 | 35 | 1 | 1 | 295 | 1 | 296 | 1.7e-147 | 531.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene27404.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27404.t1 | MTR_0558s0020 | 87.838 | 296 | 35 | 1 | 1 | 295 | 1 | 296 | 0.0 | 550 |
| MS.gene27404.t1 | MTR_1g083640 | 83.099 | 284 | 32 | 1 | 1 | 268 | 1 | 284 | 4.11e-179 | 495 |
| MS.gene27404.t1 | MTR_8g446020 | 73.898 | 295 | 38 | 1 | 1 | 295 | 1 | 256 | 1.99e-159 | 444 |
| MS.gene27404.t1 | MTR_6g090505 | 85.217 | 230 | 34 | 0 | 65 | 294 | 1 | 230 | 3.82e-149 | 422 |
| MS.gene27404.t1 | MTR_2g014100 | 82.041 | 245 | 35 | 1 | 1 | 236 | 1 | 245 | 1.75e-148 | 416 |
| MS.gene27404.t1 | MTR_0337s0040 | 71.648 | 261 | 34 | 2 | 1 | 260 | 1 | 222 | 5.03e-130 | 369 |
| MS.gene27404.t1 | MTR_3g008240 | 84.729 | 203 | 30 | 1 | 1 | 202 | 1 | 203 | 8.88e-125 | 354 |
| MS.gene27404.t1 | MTR_4g040430 | 84.158 | 202 | 28 | 1 | 1 | 202 | 1 | 198 | 1.93e-124 | 353 |
| MS.gene27404.t1 | MTR_5g071590 | 73.889 | 180 | 34 | 2 | 1 | 167 | 1 | 180 | 2.17e-95 | 280 |
| MS.gene27404.t1 | MTR_1g055240 | 87.324 | 142 | 18 | 0 | 91 | 232 | 3 | 144 | 7.04e-92 | 269 |
| MS.gene27404.t1 | MTR_0019s0290 | 81.579 | 152 | 28 | 0 | 82 | 233 | 9 | 160 | 7.18e-91 | 267 |
| MS.gene27404.t1 | MTR_1281s0010 | 81.651 | 109 | 20 | 0 | 19 | 127 | 1 | 109 | 5.31e-61 | 189 |
| MS.gene27404.t1 | MTR_4g008820 | 83.750 | 80 | 12 | 1 | 1 | 79 | 125 | 204 | 4.37e-34 | 131 |
| MS.gene27404.t1 | MTR_8g046060 | 77.273 | 88 | 11 | 2 | 1 | 79 | 91 | 178 | 2.81e-33 | 122 |
| MS.gene27404.t1 | MTR_6g051425 | 32.129 | 249 | 154 | 4 | 40 | 288 | 22 | 255 | 4.82e-32 | 121 |
| MS.gene27404.t1 | MTR_1g070355 | 27.888 | 251 | 162 | 4 | 41 | 291 | 80 | 311 | 2.68e-20 | 91.3 |
| MS.gene27404.t1 | MTR_0402s0040 | 25.405 | 185 | 119 | 4 | 49 | 233 | 40 | 205 | 9.73e-16 | 77.4 |
| MS.gene27404.t1 | MTR_0337s0050 | 75.000 | 36 | 9 | 0 | 259 | 294 | 14 | 49 | 4.26e-14 | 66.6 |
| MS.gene27404.t1 | MTR_1g013060 | 29.954 | 217 | 131 | 8 | 40 | 253 | 29 | 227 | 1.29e-13 | 69.3 |
| MS.gene27404.t1 | MTR_4g035820 | 22.378 | 286 | 189 | 7 | 17 | 295 | 61 | 320 | 3.52e-13 | 70.1 |
| MS.gene27404.t1 | MTR_7g074790 | 27.179 | 195 | 101 | 6 | 31 | 222 | 3 | 159 | 4.65e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27404.t1 | AT4G38180 | 30.075 | 266 | 158 | 6 | 32 | 292 | 70 | 312 | 6.85e-28 | 113 |
| MS.gene27404.t1 | AT5G28530 | 23.846 | 260 | 168 | 6 | 40 | 287 | 60 | 301 | 6.06e-16 | 78.6 |
| MS.gene27404.t1 | AT5G28530 | 23.846 | 260 | 168 | 6 | 40 | 287 | 60 | 301 | 6.06e-16 | 78.6 |
| MS.gene27404.t1 | AT5G28530 | 23.846 | 260 | 168 | 6 | 40 | 287 | 60 | 301 | 6.13e-16 | 78.6 |
| MS.gene27404.t1 | AT1G10240 | 23.413 | 252 | 169 | 7 | 49 | 295 | 62 | 294 | 3.10e-14 | 73.2 |
| MS.gene27404.t1 | AT1G10240 | 23.413 | 252 | 169 | 7 | 49 | 295 | 62 | 294 | 3.10e-14 | 73.2 |
| MS.gene27404.t1 | AT4G15090 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 1.89e-12 | 68.2 |
| MS.gene27404.t1 | AT4G15090 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 1.89e-12 | 68.2 |
| MS.gene27404.t1 | AT4G15090 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 1.89e-12 | 68.2 |
| MS.gene27404.t1 | AT4G15090 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 1.89e-12 | 68.2 |
| MS.gene27404.t1 | AT4G15090 | 25.506 | 247 | 163 | 6 | 50 | 295 | 66 | 292 | 1.89e-12 | 68.2 |
| MS.gene27404.t1 | AT1G76320 | 24.498 | 249 | 152 | 8 | 50 | 294 | 12 | 228 | 4.92e-12 | 66.6 |
| MS.gene27404.t1 | AT1G76320 | 23.077 | 247 | 158 | 6 | 50 | 294 | 12 | 228 | 6.17e-12 | 66.6 |
| MS.gene27404.t1 | AT1G76320 | 23.077 | 247 | 158 | 6 | 50 | 294 | 12 | 228 | 6.17e-12 | 66.6 |
| MS.gene27404.t1 | AT1G76320 | 24.498 | 249 | 152 | 8 | 50 | 294 | 12 | 228 | 6.69e-12 | 66.2 |
| MS.gene27404.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 127 | 295 | 108 | 263 | 1.56e-11 | 65.5 |
| MS.gene27404.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 127 | 295 | 108 | 263 | 1.56e-11 | 65.5 |
Find 41 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | 6.4:-50151076 | MS.gene27404:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.341758 | 6.4:+50151026 | None:intergenic |
| AACAATAAATATCGGCCATT+AGG | 0.388497 | 6.4:-50151019 | MS.gene27404:CDS |
| ACCGAACAAACAATAAATAT+CGG | 0.389395 | 6.4:-50151027 | MS.gene27404:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 6.4:+50151556 | None:intergenic |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 6.4:-50151785 | MS.gene27404:CDS |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 6.4:-50151714 | MS.gene27404:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 6.4:-50151379 | MS.gene27404:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 6.4:-50151497 | MS.gene27404:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 6.4:-50151514 | MS.gene27404:CDS |
| CGTTATGTTTGTAACAAAGA+AGG | 0.449439 | 6.4:-50151631 | MS.gene27404:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 6.4:-50151349 | MS.gene27404:CDS |
| ATCTGAGAGTGGACAATTGA+CGG | 0.452529 | 6.4:-50151860 | MS.gene27404:CDS |
| AGTATAAAATACTAACCTAA+TGG | 0.461714 | 6.4:+50151004 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 6.4:-50151665 | MS.gene27404:CDS |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 6.4:-50151304 | MS.gene27404:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 6.4:+50151520 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 6.4:-50151727 | MS.gene27404:CDS |
| TCTAATGACAACTCGTCAAA+AGG | 0.488137 | 6.4:-50151236 | MS.gene27404:CDS |
| CTCAAGTAGCACAAATGCTC+TGG | 0.489977 | 6.4:+50151409 | None:intergenic |
| TTTAAGTCCGAAAATTGAAA+AGG | 0.490057 | 6.4:+50151265 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 6.4:-50151671 | MS.gene27404:CDS |
| CGTCAAAAGGAGATGGTGGC+TGG | 0.492100 | 6.4:-50151223 | MS.gene27404:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | 6.4:-50151317 | MS.gene27404:CDS |
| TGTCTTCTGCAGCATCAACT+TGG | 0.502977 | 6.4:+50151131 | None:intergenic |
| AAATGCTCTGGTTTATGTAA+TGG | 0.504865 | 6.4:+50151421 | None:intergenic |
| TGTCTATAAGATATATAAGT+GGG | 0.514682 | 6.4:-50151461 | MS.gene27404:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 6.4:-50151620 | MS.gene27404:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 6.4:-50151619 | MS.gene27404:CDS |
| CATTTAGTGATAAAGAATCA+TGG | 0.546005 | 6.4:-50151795 | MS.gene27404:CDS |
| AATGAAGCTCTCTCGAAACC+AGG | 0.565261 | 6.4:-50151169 | MS.gene27404:CDS |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 6.4:-50151836 | MS.gene27404:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 6.4:-50151301 | MS.gene27404:CDS |
| ATGTCTATAAGATATATAAG+TGG | 0.572287 | 6.4:-50151462 | MS.gene27404:CDS |
| ATATTAACTTCATCTGAGAG+TGG | 0.588105 | 6.4:-50151871 | MS.gene27404:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | 6.4:-50151310 | MS.gene27404:CDS |
| GACAACTCGTCAAAAGGAGA+TGG | 0.599038 | 6.4:-50151230 | MS.gene27404:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | 6.4:-50151316 | MS.gene27404:CDS |
| AACTCGTCAAAAGGAGATGG+TGG | 0.603142 | 6.4:-50151227 | MS.gene27404:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 6.4:+50151571 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 6.4:-50151296 | MS.gene27404:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTCTATAAGATATATAAG+TGG | - | chr6.4:50151433-50151452 | MS.gene27404:CDS | 20.0% |
| !! | CAATTCACAAAATAAGAAAT+CGG | - | chr6.4:50151224-50151243 | MS.gene27404:CDS | 20.0% |
| !! | TGTCTATAAGATATATAAGT+GGG | - | chr6.4:50151434-50151453 | MS.gene27404:CDS | 20.0% |
| !! | TTTAATTATGCATATAACAC+TGG | - | chr6.4:50151168-50151187 | MS.gene27404:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | - | chr6.4:50151230-50151249 | MS.gene27404:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | chr6.4:50151868-50151887 | MS.gene27404:CDS | 25.0% |
| ! | CATTTAGTGATAAAGAATCA+TGG | - | chr6.4:50151100-50151119 | MS.gene27404:CDS | 25.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | - | chr6.4:50151059-50151078 | MS.gene27404:CDS | 25.0% |
| ! | TTTAAGTCCGAAAATTGAAA+AGG | + | chr6.4:50151633-50151652 | None:intergenic | 25.0% |
| !! | GAATCTTCCTTTTCAATTTT+CGG | - | chr6.4:50151623-50151642 | MS.gene27404:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | chr6.4:50151220-50151239 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | chr6.4:50151546-50151565 | MS.gene27404:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | - | chr6.4:50151276-50151295 | MS.gene27404:CDS | 30.0% | |
| AACAATAAATATCGGCCATT+AGG | - | chr6.4:50151876-50151895 | MS.gene27404:CDS | 30.0% | |
| AGAACATCTTATGAAGTGAT+GGG | - | chr6.4:50151579-50151598 | MS.gene27404:CDS | 30.0% | |
| ATATTAACTTCATCTGAGAG+TGG | - | chr6.4:50151024-50151043 | MS.gene27404:CDS | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | - | chr6.4:50151819-50151838 | MS.gene27404:CDS | 30.0% | |
| CGTTATGTTTGTAACAAAGA+AGG | - | chr6.4:50151264-50151283 | MS.gene27404:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | chr6.4:50151872-50151891 | None:intergenic | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | chr6.4:50151365-50151384 | MS.gene27404:CDS | 30.0% | |
| TAAAGAATCATGGAAGTCTT+TGG | - | chr6.4:50151110-50151129 | MS.gene27404:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | chr6.4:50151275-50151294 | MS.gene27404:CDS | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | + | chr6.4:50151747-50151766 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | chr6.4:50151477-50151496 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | chr6.4:50151398-50151417 | MS.gene27404:CDS | 30.0% |
| !!! | AAGACATAGCCAGTATTTTT+TGG | - | chr6.4:50151781-50151800 | MS.gene27404:CDS | 30.0% |
| !!! | AGACATAGCCAGTATTTTTT+GGG | - | chr6.4:50151782-50151801 | MS.gene27404:CDS | 30.0% |
| ATAACACTGGATTCTCTGTT+AGG | - | chr6.4:50151181-50151200 | MS.gene27404:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr6.4:50151516-50151535 | MS.gene27404:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | chr6.4:50151364-50151383 | MS.gene27404:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | chr6.4:50151359-50151378 | MS.gene27404:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | - | chr6.4:50151578-50151597 | MS.gene27404:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | chr6.4:50151301-50151320 | MS.gene27404:CDS | 35.0% | |
| TCTAATGACAACTCGTCAAA+AGG | - | chr6.4:50151659-50151678 | MS.gene27404:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | chr6.4:50151342-50151361 | None:intergenic | 35.0% | |
| ATCTGAGAGTGGACAATTGA+CGG | - | chr6.4:50151035-50151054 | MS.gene27404:CDS | 40.0% | |
| TCCATCTGCCCAAAAAATAC+TGG | + | chr6.4:50151793-50151812 | None:intergenic | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | chr6.4:50151315-50151334 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTCA+AGG | - | chr6.4:50151585-50151604 | MS.gene27404:CDS | 40.0% |
| AACTCGTCAAAAGGAGATGG+TGG | - | chr6.4:50151668-50151687 | MS.gene27404:CDS | 45.0% | |
| AATGAAGCTCTCTCGAAACC+AGG | - | chr6.4:50151726-50151745 | MS.gene27404:CDS | 45.0% | |
| AGAACTGGACTGTCACATGT+TGG | + | chr6.4:50151327-50151346 | None:intergenic | 45.0% | |
| CTCAAGTAGCACAAATGCTC+TGG | + | chr6.4:50151489-50151508 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | chr6.4:50151369-50151388 | MS.gene27404:CDS | 45.0% | |
| GACAACTCGTCAAAAGGAGA+TGG | - | chr6.4:50151665-50151684 | MS.gene27404:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | + | chr6.4:50151767-50151786 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | chr6.4:50151378-50151397 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr6.4:50151381-50151400 | MS.gene27404:CDS | 45.0% |
| !!! | GCCAGTATTTTTTGGGCAGA+TGG | - | chr6.4:50151789-50151808 | MS.gene27404:CDS | 45.0% |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | - | chr6.4:50151591-50151610 | MS.gene27404:CDS | 50.0% |
| ! | CGTCAAAAGGAGATGGTGGC+TGG | - | chr6.4:50151672-50151691 | MS.gene27404:CDS | 55.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | - | chr6.4:50151594-50151613 | MS.gene27404:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | chr6.4:50151599-50151618 | MS.gene27404:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 50151015 | 50151902 | 50151015 | ID=MS.gene27404 |
| chr6.4 | mRNA | 50151015 | 50151902 | 50151015 | ID=MS.gene27404.t1;Parent=MS.gene27404 |
| chr6.4 | exon | 50151015 | 50151902 | 50151015 | ID=MS.gene27404.t1.exon1;Parent=MS.gene27404.t1 |
| chr6.4 | CDS | 50151015 | 50151902 | 50151015 | ID=cds.MS.gene27404.t1;Parent=MS.gene27404.t1 |
| Gene Sequence |
| Protein sequence |
