AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27440.t1 MTR_6g071625 97.872 141 3 0 1 141 200 340 3.55e-100 292
MS.gene27440.t1 MTR_3g064840 64.341 129 34 3 1 117 272 400 1.05e-44 152
MS.gene27440.t1 MTR_8g066310 54.412 136 47 2 1 121 171 306 1.38e-39 136
MS.gene27440.t1 MTR_5g029470 89.706 68 7 0 1 68 217 284 1.31e-36 130
MS.gene27440.t1 MTR_1g021520 84.848 66 10 0 1 66 124 189 2.84e-34 121
MS.gene27440.t1 MTR_7g069660 55.294 85 31 1 2 79 70 154 1.06e-24 97.4
MS.gene27440.t1 MTR_0223s0040 65.517 58 20 0 2 59 17 74 3.47e-23 92.4
MS.gene27440.t1 MTR_1g112370 63.793 58 21 0 2 59 21 78 1.61e-22 91.3
MS.gene27440.t1 MTR_2g023580 58.571 70 29 0 2 71 54 123 2.86e-22 89.0
MS.gene27440.t1 MTR_2g084230 68.966 58 18 0 2 59 96 153 1.43e-21 88.6
MS.gene27440.t1 MTR_8g086410 67.241 58 19 0 2 59 108 165 1.79e-21 89.4
MS.gene27440.t1 MTR_8g086410 67.241 58 19 0 2 59 110 167 1.79e-21 89.4
MS.gene27440.t1 MTR_2g084230 68.966 58 18 0 2 59 96 153 2.36e-21 88.6
MS.gene27440.t1 MTR_2g016220 65.517 58 20 0 2 59 88 145 4.13e-20 85.1
MS.gene27440.t1 MTR_2g016220 65.517 58 20 0 2 59 88 145 4.44e-20 85.1
MS.gene27440.t1 MTR_7g098250 57.534 73 29 1 4 76 263 333 6.56e-20 85.1
MS.gene27440.t1 MTR_7g115530 56.164 73 30 1 4 76 219 289 1.23e-19 83.2
MS.gene27440.t1 MTR_7g115530 56.164 73 30 1 4 76 219 289 1.89e-19 82.8
MS.gene27440.t1 MTR_8g086410 64.286 56 20 0 2 57 110 165 2.03e-19 83.6
MS.gene27440.t1 MTR_7g115530 56.164 73 30 1 4 76 219 289 2.98e-19 83.2
MS.gene27440.t1 MTR_1g080330 62.500 64 22 1 4 67 176 237 4.15e-19 81.6
MS.gene27440.t1 MTR_1g080330 62.500 64 22 1 4 67 176 237 5.19e-19 82.4
MS.gene27440.t1 MTR_3g450310 58.929 56 23 0 4 59 20 75 9.49e-19 79.3
MS.gene27440.t1 MTR_5g027440 67.925 53 17 0 4 56 26 78 1.11e-18 80.1
MS.gene27440.t1 MTR_7g115530 64.286 56 20 0 4 59 219 274 1.37e-18 80.9
MS.gene27440.t1 MTR_7g088070 57.576 66 26 1 5 70 52 115 2.93e-18 79.0
MS.gene27440.t1 MTR_1g053835 57.377 61 26 0 5 65 260 320 3.69e-18 80.1
MS.gene27440.t1 MTR_7g093030 60.000 55 22 0 5 59 214 268 4.12e-18 79.0
MS.gene27440.t1 MTR_7g088070 63.636 55 20 0 5 59 52 106 4.62e-18 78.6
MS.gene27440.t1 MTR_2g016220 61.290 62 20 1 2 59 88 149 4.82e-18 79.7
MS.gene27440.t1 MTR_7g093030 60.000 55 22 0 5 59 214 268 7.88e-18 79.0
MS.gene27440.t1 MTR_2g027800 58.333 60 25 0 4 63 40 99 1.25e-17 77.8
MS.gene27440.t1 MTR_1g090670 48.148 81 41 1 4 84 45 124 1.50e-17 77.4
MS.gene27440.t1 MTR_2g027860 60.000 65 24 1 5 69 51 113 1.79e-17 77.8
MS.gene27440.t1 MTR_2g027800 48.235 85 43 1 4 88 40 123 4.42e-17 76.3
MS.gene27440.t1 MTR_2g086450 52.308 65 31 0 4 68 46 110 5.93e-17 76.6
MS.gene27440.t1 MTR_6g444980 56.667 60 26 0 4 63 36 95 1.24e-16 75.5
MS.gene27440.t1 MTR_6g444980 56.667 60 26 0 4 63 36 95 1.28e-16 75.5
MS.gene27440.t1 MTR_6g032990 60.377 53 21 0 4 56 43 95 1.99e-16 75.1
MS.gene27440.t1 MTR_7g115530 63.462 52 19 0 4 55 219 270 2.66e-16 73.9
MS.gene27440.t1 MTR_5g041350 53.571 56 26 0 4 59 16 71 2.94e-16 74.3
MS.gene27440.t1 MTR_7g115530 66.000 50 17 0 4 53 219 268 4.04e-16 73.2
MS.gene27440.t1 MTR_4g081710 39.785 93 50 2 4 96 18 104 5.09e-16 73.6
MS.gene27440.t1 MTR_1g053830 49.180 61 31 0 5 65 20 80 4.09e-15 67.0
MS.gene27440.t1 MTR_7g068600 55.172 58 23 1 5 59 15 72 8.22e-14 66.6
MS.gene27440.t1 MTR_1g053800 50.847 59 29 0 5 63 179 237 9.05e-14 67.4
MS.gene27440.t1 MTR_4g113140 49.231 65 33 0 2 66 234 298 3.65e-13 65.9
MS.gene27440.t1 MTR_2g450070 39.024 82 43 2 2 77 212 292 1.68e-12 64.3
MS.gene27440.t1 MTR_2g450070 39.024 82 43 2 2 77 212 292 1.73e-12 63.9
MS.gene27440.t1 MTR_4g086835 46.053 76 39 1 2 77 273 346 1.82e-12 63.9
MS.gene27440.t1 MTR_5g017980 45.122 82 43 1 2 83 192 271 2.50e-12 63.2
MS.gene27440.t1 MTR_5g054300 52.727 55 26 0 2 56 197 251 7.05e-12 62.0
MS.gene27440.t1 MTR_1g093080 43.056 72 39 1 2 73 210 279 7.13e-12 62.0
MS.gene27440.t1 MTR_4g086835 46.053 76 38 2 2 77 273 345 4.31e-11 60.1
MS.gene27440.t1 MTR_7g093010 49.057 53 27 0 5 57 215 267 4.94e-11 59.7
MS.gene27440.t1 MTR_5g017980 45.122 82 42 2 2 83 191 269 5.49e-11 59.3
MS.gene27440.t1 MTR_5g017980 45.122 82 42 2 2 83 192 270 5.67e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27440.t1 AT5G16560 95.714 70 3 0 1 70 217 286 6.71e-41 141
MS.gene27440.t1 AT1G32240 92.647 68 5 0 1 68 211 278 7.05e-38 132
MS.gene27440.t1 AT1G32240 92.647 68 5 0 1 68 211 278 1.13e-37 132
MS.gene27440.t1 AT4G17695 82.353 68 12 0 1 68 162 229 3.46e-35 124
MS.gene27440.t1 AT5G42630 88.889 63 7 0 1 63 103 165 1.53e-34 120
MS.gene27440.t1 AT5G42630 88.889 63 7 0 1 63 103 165 6.05e-34 120
MS.gene27440.t1 AT4G04605 68.966 58 18 0 2 59 13 70 2.62e-23 91.3
MS.gene27440.t1 AT2G38300 46.341 123 51 4 2 112 53 172 4.12e-23 93.2
MS.gene27440.t1 AT2G40260 50.000 100 31 3 2 101 81 161 5.36e-23 93.6
MS.gene27440.t1 AT2G02060 72.222 54 15 0 3 56 29 82 3.65e-22 89.4
MS.gene27440.t1 AT2G02060 72.222 54 15 0 3 56 29 82 3.65e-22 89.4
MS.gene27440.t1 AT1G14600 66.102 59 20 0 3 61 23 81 2.97e-21 86.7
MS.gene27440.t1 AT2G42660 66.071 56 19 0 4 59 51 106 9.03e-20 82.8
MS.gene27440.t1 AT4G04580 59.322 59 24 0 4 62 15 73 9.49e-20 80.9
MS.gene27440.t1 AT5G45580 67.925 53 17 0 4 56 8 60 2.17e-19 82.0
MS.gene27440.t1 AT5G45580 67.925 53 17 0 4 56 24 76 2.74e-19 82.0
MS.gene27440.t1 AT4G04555 55.738 61 27 0 2 62 13 73 1.09e-18 76.3
MS.gene27440.t1 AT4G28610 52.055 73 35 0 5 77 227 299 1.21e-18 81.6
MS.gene27440.t1 AT4G13640 44.318 88 47 1 4 91 38 123 2.31e-18 79.7
MS.gene27440.t1 AT4G13640 44.318 88 47 1 4 91 38 123 2.33e-18 79.7
MS.gene27440.t1 AT3G04450 54.930 71 30 1 5 75 240 308 2.77e-18 80.5
MS.gene27440.t1 AT3G04450 54.930 71 30 1 5 75 200 268 2.82e-18 80.5
MS.gene27440.t1 AT3G04450 54.930 71 30 1 5 75 200 268 2.82e-18 80.5
MS.gene27440.t1 AT5G18240 43.333 90 49 1 4 91 46 135 4.79e-18 79.7
MS.gene27440.t1 AT5G18240 43.333 90 49 1 4 91 46 135 4.79e-18 79.7
MS.gene27440.t1 AT5G18240 43.333 90 49 1 4 91 46 135 5.07e-18 79.7
MS.gene27440.t1 AT5G18240 43.333 90 49 1 4 91 46 135 5.16e-18 79.7
MS.gene27440.t1 AT5G18240 43.333 90 49 1 4 91 46 135 5.16e-18 79.7
MS.gene27440.t1 AT2G01060 59.091 66 25 1 5 70 17 80 6.87e-18 78.2
MS.gene27440.t1 AT3G24120 46.053 76 41 0 4 79 42 117 8.51e-18 78.2
MS.gene27440.t1 AT3G24120 46.053 76 41 0 4 79 42 117 8.85e-18 78.2
MS.gene27440.t1 AT3G04450 58.462 65 25 1 5 69 240 302 9.99e-18 78.2
MS.gene27440.t1 AT3G12730 58.333 60 25 0 4 63 24 83 1.45e-17 76.6
MS.gene27440.t1 AT3G04030 44.737 76 42 0 4 79 46 121 2.28e-17 77.8
MS.gene27440.t1 AT3G04030 44.737 76 42 0 4 79 46 121 2.71e-17 77.8
MS.gene27440.t1 AT5G29000 63.636 55 20 0 5 59 233 287 3.75e-17 76.6
MS.gene27440.t1 AT5G29000 63.636 55 20 0 5 59 190 244 3.96e-17 77.0
MS.gene27440.t1 AT5G29000 63.636 55 20 0 5 59 190 244 3.96e-17 77.0
MS.gene27440.t1 AT5G29000 63.636 55 20 0 5 59 233 287 5.16e-17 77.0
MS.gene27440.t1 AT1G79430 56.667 60 26 0 4 63 35 94 1.22e-16 75.5
MS.gene27440.t1 AT2G20400 50.667 75 37 0 5 79 233 307 1.80e-16 75.5
MS.gene27440.t1 AT2G20400 57.812 64 27 0 5 68 233 296 3.72e-16 74.3
MS.gene27440.t1 AT5G06800 56.364 55 24 0 5 59 194 248 4.79e-16 73.2
MS.gene27440.t1 AT5G06800 56.364 55 24 0 5 59 194 248 1.62e-15 72.4
MS.gene27440.t1 AT5G06800 56.364 55 24 0 5 59 194 248 1.63e-15 72.4
MS.gene27440.t1 AT2G06020 61.538 52 20 0 5 56 88 139 1.68e-15 72.0
MS.gene27440.t1 AT3G13040 61.818 55 21 0 5 59 243 297 1.85e-15 72.4
MS.gene27440.t1 AT3G13040 61.818 55 21 0 5 59 243 297 1.85e-15 72.4
MS.gene27440.t1 AT3G13040 61.818 55 21 0 5 59 243 297 1.85e-15 72.4
MS.gene27440.t1 AT1G69580 45.205 73 37 1 4 73 35 107 3.62e-15 71.2
MS.gene27440.t1 AT1G69580 45.205 73 37 1 4 73 35 107 4.19e-15 71.2
MS.gene27440.t1 AT3G04030 43.421 76 42 1 4 79 46 120 1.02e-14 70.5
MS.gene27440.t1 AT2G03500 46.377 69 37 0 2 70 232 300 2.97e-13 66.2
MS.gene27440.t1 AT1G32240 93.548 31 2 0 38 68 1 31 4.33e-12 60.1
MS.gene27440.t1 AT1G25550 50.000 64 30 1 2 65 208 269 9.33e-12 61.6
MS.gene27440.t1 AT1G49190 44.828 58 29 1 2 56 214 271 1.55e-11 61.2
MS.gene27440.t1 AT4G37180 37.931 87 42 1 5 79 219 305 1.86e-11 60.8
MS.gene27440.t1 AT4G37180 37.931 87 42 1 5 79 212 298 1.91e-11 60.8
MS.gene27440.t1 AT4G31920 50.877 57 27 1 2 58 182 237 2.42e-11 60.8
MS.gene27440.t1 AT1G68670 42.500 80 44 1 2 81 214 291 3.80e-11 60.1
MS.gene27440.t1 AT5G44190 50.000 56 27 1 2 57 146 200 5.38e-11 59.7
MS.gene27440.t1 AT3G62670 39.744 78 41 2 2 75 210 285 6.63e-11 59.3
MS.gene27440.t1 AT5G44190 50.000 56 27 1 2 57 146 200 6.90e-11 59.3
MS.gene27440.t1 AT1G13300 52.727 55 26 0 2 56 180 234 7.34e-11 59.3

Find 27 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAGCTCCAAAACTGATTT+AGG 0.195719 6.4:+49425468 None:intergenic
GCTACACTTCCAACATTCTA+TGG 0.201212 6.4:-49423494 MS.gene27440:CDS
AGCTACTCCTAAATCAGTTT+TGG 0.286064 6.4:-49425475 MS.gene27440:CDS
TTTGATCATGAGGTTGTGTT+AGG 0.298913 6.4:+49423553 None:intergenic
GAACTAGTCCATCGCATTCT+CGG 0.354623 6.4:+49425902 None:intergenic
GCATCTCTAGATCATGATAT+TGG 0.355771 6.4:-49423516 MS.gene27440:CDS
TCACAAAAGTTATACATATA+TGG 0.358550 6.4:+49414937 None:intergenic
GACAAGCCAGCAGCTTCTTC+AGG 0.364958 6.4:-49424501 MS.gene27440:intron
ACATGGACACAAGGTAGTTC+TGG 0.378399 6.4:-49416110 MS.gene27440:CDS
TCATGTCAAGAGTCATTTAC+AGG 0.385177 6.4:-49425421 MS.gene27440:intron
GTTCACGCCGTTGAGCTTCT+CGG 0.392269 6.4:-49425863 MS.gene27440:CDS
AGAGAAGAGATATACTTTCA+TGG 0.417461 6.4:+49425837 None:intergenic
TATGGGCTAATTCTTCTTCA+AGG 0.425004 6.4:-49423476 MS.gene27440:intron
TTCCAATGTGGTGAGTTGAT+AGG 0.454476 6.4:+49416054 None:intergenic
TTGATCATGAGGTTGTGTTA+GGG 0.490043 6.4:+49423554 None:intergenic
CTACACTTCCAACATTCTAT+GGG 0.540264 6.4:-49423493 MS.gene27440:CDS
CCACATTGGAAAGCTTCCTC+AGG 0.553225 6.4:-49416042 MS.gene27440:intron
AGAATTAGCCCATAGAATGT+TGG 0.553625 6.4:+49423485 None:intergenic
ATCCTATCAACTCACCACAT+TGG 0.574962 6.4:-49416056 MS.gene27440:CDS
ATTATACCTGAAGAAGCTGC+TGG 0.580120 6.4:+49424495 None:intergenic
TGAGGGCTCCGAGAATGCGA+TGG 0.608831 6.4:-49425910 MS.gene27440:CDS
ACATATATGGACAAATCAGT+AGG 0.622273 6.4:+49414950 None:intergenic
TGACTGGCAGTAATGGAACA+TGG 0.648272 6.4:-49416127 MS.gene27440:intron
CCTGAGGAAGCTTTCCAATG+TGG 0.649852 6.4:+49416042 None:intergenic
ATGGCCGCCGAGAAGCTCAA+CGG 0.658735 6.4:+49425856 None:intergenic
AGTAATGGAACATGGACACA+AGG 0.680964 6.4:-49416119 MS.gene27440:CDS
CACGCCGTTGAGCTTCTCGG+CGG 0.701910 6.4:-49425860 MS.gene27440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.4 gene 49414946 49425933 49414946 ID=MS.gene27440
chr6.4 mRNA 49414946 49425933 49414946 ID=MS.gene27440.t1;Parent=MS.gene27440
chr6.4 exon 49425852 49425933 49425852 ID=MS.gene27440.t1.exon1;Parent=MS.gene27440.t1
chr6.4 CDS 49425852 49425933 49425852 ID=cds.MS.gene27440.t1;Parent=MS.gene27440.t1
chr6.4 exon 49425422 49425498 49425422 ID=MS.gene27440.t1.exon2;Parent=MS.gene27440.t1
chr6.4 CDS 49425422 49425498 49425422 ID=cds.MS.gene27440.t1;Parent=MS.gene27440.t1
chr6.4 exon 49424502 49424547 49424502 ID=MS.gene27440.t1.exon3;Parent=MS.gene27440.t1
chr6.4 CDS 49424502 49424547 49424502 ID=cds.MS.gene27440.t1;Parent=MS.gene27440.t1
chr6.4 exon 49423477 49423597 49423477 ID=MS.gene27440.t1.exon4;Parent=MS.gene27440.t1
chr6.4 CDS 49423477 49423597 49423477 ID=cds.MS.gene27440.t1;Parent=MS.gene27440.t1
chr6.4 exon 49416043 49416139 49416043 ID=MS.gene27440.t1.exon5;Parent=MS.gene27440.t1
chr6.4 CDS 49416043 49416139 49416043 ID=cds.MS.gene27440.t1;Parent=MS.gene27440.t1
chr6.4 exon 49414946 49414975 49414946 ID=MS.gene27440.t1.exon6;Parent=MS.gene27440.t1
chr6.4 CDS 49414946 49414975 49414946 ID=cds.MS.gene27440.t1;Parent=MS.gene27440.t1
Gene Sequence

>MS.gene27440

ATGAGGGCTCCGAGAATGCGATGGACTAGTTCGCTTCATAATCGATTTGTTCACGCCGTTGAGCTTCTCGGCGGCCATGAAAGTATATCTCTTCTCTTGTTGAATCTCTCCCTTTTAATATGAGTATAGATCATCTGCACCAATACAATTGGATTGTTGGTCTGTATGTTTAGATTGACGGTGAATTTGACAAAATCTCAGGGTTGAATCTCTGAAGTGAAGTTTTTAAAAGAAACTGAACACTTCACTCTGATTTCATCAATCTCACCGTTAATCTAAACATACACTTTATCGATTATCGATTGTAGTGTCGTTGGATCTGAGTGTATCAAGATGTATATATGTGTGTGAGTGTGAATACTATACATATATATCTATAAAAAGTAAATTTTTGAACTCTTATGCATTTTTCTTTCTTTCAATTTTGGATGGTAGGAGCTACTCCTAAATCAGTTTTGGAGCTAATGGATGTGAAAGATCTAACACTAGCTCATGTCAAGAGTCATTTACAGGTATATAACTAATTCTTTTCTTACCCTATTTTTCTTCACATATAAAACAAAATTACAAACACACACACACACTCTCTTTATGTGTGACTGAAGTGGATTGTCCAAAGTCTACTCTATCTTCTAAGAGGCACAAGAGCCAAGGCATGCAGTGATTGAATGAGTGAGATGAACAGTTGAATCTGTTTGTTTTGATGCACAGAACCAGACAAGTTCTACTTTTCATGAGAGAGAGAAAAAAAAGTTAGAAAAAGAAAGTAAGAAAAGTAGCCATCATTTCTCAATAATAGAACCAATTACTTACCTTACCAAACATATCAACCTTATTGTTATTATTTAATTGAACAAACCCCTAGAGGAAAAAGAAGCATCTGATTTCATTATTTTATTACTACTTGTTTTTCTTTCTATATTTGATCTTTGGAAAAAGATTGATCAATATTGAAAACCCTTTGAAATCTTGAAAGAGAAAGAATATATCGAGCGTGATGTGATATGTATGATATGTTACTGATGTGATAAAAAGAGAAAGATAGAAGGAAATGATGTGATGAATGAATGTAAAAGAATTAGAGGCATAGACGAATCATCGTTTTTTATCTTTTCAATACACAAATATAGATTTTCTAGAATCTAGATTTTCCGTTGCAAAAAAAAAAAAAACCTCACGATGACACTCTGGATTGGATCTCAGTCATTGAGTTAAGATCGATGGATTCACATTGCACCTTAACTAAATTATTATTAAACAATCTCTACCATTAATTTAAAATCGAACGATTGAAATTGATAGCAGCATATACTAACCACACAATTTTCTCATATATTATTTATTTGTATATTTTAATCTTAATTGTCCTTCTATTGGTTTCTGCAGATGTATAGAACTGTTAAAAACACTGACAAGCCAGCAGCTTCTTCAGGTATAATTCATATTGAAATTACCAAGTTGTTTTAGTACTCCCTCCGTCCCGGAATATCTGACCCATTTTCTAAAAAAAAGTGTCCCAAAATAACTGAGTCATTTCAATTTCCAATACAACTTTCATCATTCCCAATACAATATAACTTTAATTAATACTACTTTCATCATTCTCAATATAATATATTCCACTTTCCCCTTTATTTATACATTTTTTTTTAATCTGTGTGAAAAACCAAACTGGCTCAGATAAAATGGGACGGAGGGAGTAGTATTTTACCTAATACCTAATGTATAATTTATACTAAATAATTCCTTGGTCTTTGTTCCAAAATTTGAAGTCCACTTTACTTAATTCCTGTGAAGATTCCTCTCACAAAACTTTATGACTCACTGTATTAATCCATCAATATTTAAACATTTTTTTCTTTACATATATAAAGTGAAGGATCTTACTAACAAACGCCTAATAGCATTGTTTAATAAAGCCCCAAAGAAAAAAATTATTTAGAAAATATAAGTGAAACACATGTAAAAGTCATAACTATACAATTTTAAAATGGGTCATGCTAACTTGTATCCTTAGGATACATAATAAAGATTTCATAAATAGTAAATATTTATTACAAAATATTAATTTTCAATCTCTAGCAGAATGAGTTGGCACAATTTCCTTGATAAAATTTTCACTTTAAGTTCTTTAACATGTGCCCTAAGTGCACAAATTAACAATTCCCTTAAATTTACTACTTTTAAATGCTTAAACAATGCCCTAAATACACTTGTTAACATTTTTCTAAAGTTAATTATTAAAATGTACAAACTAAGTGAACATTTATTTCAATTTCAAATTTGTCTACAATACTATAATTTTCTTAATGGTTGTATATAATGCAATGTAACAGACGGAGATGAGAATTTCATGTCCCTAACACAACCTCATGATCAAAATAAGAACTATAATGCATCTCTAGATCATGATATTGGCTACACTTCCAACATTCTATGGGCTAATTCTTCTTCAAGGTACCATTTCTTAATTTCTTGCTTTAGTATTTTTGAATATTTGAGAAATTATATATATATATATATATATATATATATATATATATATATATATATATATGCATGAATGCATGGTTACATCCATTCTTTTGTGAAACACAAAAGTGCTTTCTTTCTGACAACTTTAGCTTTTTTCATACAACTTCACCAATTCTGCTTTTTTAGATCAAAGTAGCTAGTGTCCTCATATGTGTGATACCCCTTTTTGGTACAAGTTCTTTAAATGTACAAAAAGATAAAAAAAAAAAAATTATTAACCTAGATGAATTCTTAGTGGTTTTGTGCAAATGAACATATGTTAAGAGAAAAATATATAAAGACCTAACGATTGTTGTATACTTTCTATGGTTAACTTACTCAATTTTAGCACAACAGATTTTCCTCTCTATATTAACTTGTTTACTTTTTTACCTTTTTTACAATTGAAGGGAGTCATTTTTTTCCTCTCTATTATATGTAGTGTCTTAATATCTAGTTTAACTAAAGGTGTTAATTGAAGTTTGTAAAAAAACTCATAGACATGCCTAGATAATTTTTTTAAGCCAAAATATAATTTGTACAAAATGTACAACTATAAAAATAAATTATGAAATGGATGAGATGCTTCACCCAGACAATATACTAATTTGAAATTATTTGAAAAACCAAACAAAAAACACCTTCATGCAATAAATCAATACCAAGATCTCCATTGATATGATTCGAACTTATCTCTTGAGGTTATAAAGTTAAAACTCCTATCACCATACAGACACCTCATAGAGAATATGAATTATTCAAAATCTATTACATTCTATGAAGCATGTGTACGGACACGGATACGGACATCGAGCACGACACAAATACTGACACGTCGACACCTTAATAATTTAAGAAAACCATATAATTCAATGTAATAATATGTGTCGGTGTCGTTAGATACCTAATTCAAGAAGTATTTGTGCTTCATGTAATAGGACATTGCATTACATTGATCTAAAACATGTGTCTTGGTCTTTGGGATTACAATATGGATAACATACAATAAGATATCCTATTTTTTGGAAATAACATTAACATTAAGAAATAGTGACATTTAAAGACATTAGTGTTGAAGTATAAAGTAAAAATCACACAGATTCATACCTTTATAGGTTCCCTAAATCCTAGTAATAATTTTGATTCATAATTGAGTTGGCTTAATGTCAGTTTTGACTATCACATGCAAATAAAATGTACTCAGTCTTCACGTTATTTTTCTGCAATAGGGTTCCTTGCAAGTCAGAAAGACAGGTAGTAATCTTCAATTTACTGTCTCTTTTTCGTACACCTTTACTAGCTAGTGTGTGTTTTCATAAATTCAGATTAGTACAGAAAATAAAAGATAAAATAGGTGTGGAGTAAAGATTATTTATGCCTTTAGGTAGAGAAAATTTTTAAAATGAAAATTTATTATTAAGTGCATGTAAAAAACAGTAAGATATTCATTGATATATTTTTTATACTACTTTTTTTTTAACTGAAATTTATGAGTAAATTTATTGAGTTAATATGAATAGTTTTTAAAATATTAGGGACCATAACTATATATTTCAAAATTTGTTTAAAATACTATAAAGCCAACATTGGGTGAGAATGTGTTTTTTAAAGATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAAGAGTTAATCTTGATTATATTAGGGATAGTTTTTTAATCTGTTTTAGAAAAATAAGAGAGTTTTTTTTTTGAAGGACAAAAAATAAGAGAATTAATAATTCATTTTGATTCATCAATTCTATACCTACCTATTGTTTTAAAATATGTTCATATATAGGTCCAGTTGTATTGGATTTGACTTGTTGAACTTGACCGAATTCTTGATTTAAGCCTAAATTTAACCTAACTATGGTCTTTGGACTAGGACTCAACGAACTCAACATCTATATAAATAAAAATGCATAAATTTTTATCTTACATAGTTAAGAAATAAATTTTTTAAAAGTAAAATGCAAAACTAAGAGATGGTTTATTAATAGGTCAATCTAACAAGGATCTCTCAAAAATAGTTTAAAGATGGGATTTTTCCCATTTTTATAGTAGTAAATAAATCTTATAATAATAAATTATAAAAAAGAATATTTTATTAAAACTTATCAAAGATAAAAGGGTTCACCATTGAATCATATTCATATTAGACAACCTTTATAATATGCATTTGTAAACAAAATATAGAATAATTAAGGTATCACTTTCTTAAAAGGATTAAGGTAACATACTAGTTAGTCATCTTGACCCTTTTTGAGTTTTGCGTTTTTACACAGAAACAATGTTAATAAAATCATGTTGCTAAAAATATTAAGATAACATATGAATATATGGTTTATCTAATATAAACTTTCTAAAAATAGACCTTTATCATTTTTATCAAATAGTAAATTTTAAAACAATAAAAGAAAGAAATGTAATATTAGAGATTGATGAAAGAAAAAGAATTGAGAAACCAGTACACACTTTCTTCTGCACACACTCCAAAAACCAGCTCACACTCTCCCCCTGCCTACCCAACCTACAAACAAATATCATTCAACCACACTTAAGATAGATAAGAGTGATTGTATACATTCAGAGTCCGTTTGGTGCACATGATAGGATAAAGACAAGATATGATAAAGAACTGGATAACGACAGGATATGATAGTAACAAGATAAACACTTATCATATCCTATGTTTGGTTAACACATGATAACAAAGATGATAGCATTATTTAATCCTATCATGTGTTTGGTGCACACTTGATATTGTAAAACAAATACATATATTTAAAAAAATATATAACTTTCATATTTTTAATTGACATTAAAACAAATAATAAGTAATCTATCAAAAAAAGAAAATTATAAGTGATGAAATATATTTTTTAAAAAAGTAATCGAAATAACAAGTAACCAAATAAAAATTTGTAAAAAAAAAAAATAGTAAGTATTGAAATAAATAACTAAATTTTCATCTCGTAAAAAAAGTAACTAAATTTCATATTTTCATATTTTTTAAAAAAATTGTCACATAGAAATAAAAAAAAAAAACGGAAGCACACGGGAAATGGGAAAACAATGAAATAGTTGCAAAGAATGGATGGAAGCACATCGAAATTTGGAAAAACAAACATATGAGTAAAAATTGATTTGGAAAAACAATTTTGAGTAAGGAATCATGGATTGTATACGATTGGATAATAACCCATCAATTGAAAAACAAAGAACTGACATGGATTACGCAAACAAAGAGTTGGGAAAAAATTGGAATGGTTATGAAGAATGGAAGCACACACATGGTAGATATGTAGAGAATGGAGTCCATAGAGTAATTGTAGAGAATAAAGTCAATAGATTTGTGATTTGGTGGGAGTCGAAGGAGGGTGAAGGGTGGGAATAGAAAGCACTTGATAGGATAGGATTAGTAATGGGTTAAATTTATCCTATCCTGTTGGTGCATCAAACAACATATATAGATGGGATATGATAGTTTTATCATATCCTGTCTCTTTATTCTGCGCACCAAACGGACCCTCAAAGATAGAAGCAAAGATAGAATGTGTGTGTCTAAAATGGACCATCATACATATATTAGACTGAACTTAATTACTTAAAGCCGATGTAGACATTCAAAGATTTACATATTAATCTGATAGTAAAATAAAAGCACACTTTAATTCTCTCAAAAAAAAAAGCACACTTTAATGATTGTTTTATATTTTAATATACCAAGTTTAAATTTAAACTGTTTGATTTTGATCAAACGATTACTGATGACCACTGCGTAAATACAGTAAAATATTGATCATAGGGAAACGTGGTCCATTAATAATACAGGTTTGGAGTCCATTTTTAAGATTGAACCTTTATGTTGTTTAAAAAACAAACAAAATTCAACATTTATGCTCTCTACAAATTTCATCTCCTCTAGGAATGGCATTGGGAACAATGCATCCACTCCTAAGTGACCAAATTGCCTAAACAAGTGTCATATAGACATATCTTCCTCCCTTTTTACCTCTCTCAATTGTTGTTAACCACCTAAACTGAACAAAGAGATCATATAGGCTAAGGATGACAAAAGAACCCGCACCCGTGGATATATGCGGATAAAATTCGCCACAAACATGTGGTGGATACATTGATAGATATTCATGGATAAAATAAACAGATATTTTAACTACCCGTTATTTAATGGATACAGATGTAGATTTGATGGTATCTAACGGGACAAACATGAATATCATACTATCCGCGCTAATGTATATCCATTTACATATCTAGTATAGACATAGTGTAACATCCCAAACTGCTTTAAGGATTGTCGACTGACCCCACAAACCAACACAAGTCTTTTCAGCGTGTTTTGTCCTCACTCGCACGCTTACCGGGAAACTTCTCAGTAGGTCACCCGTCCCAAAATTTCTGCAAGTCAAGCACGCTTAACTGTGAAGTTCTTGTGGAATGAGCTACCAAAAAGAAGATGATCTTGTTTATATTGGTAGTACCAAACAATTCTTATAAGTCTTCCTTCAACCATGCAGTCCCGTACCTGCACGGCCTCAGGATCTCTCTCATTTCGATGTGATTCAGTTTTGCCAAGAAACATGCTAGGAGCCGGACCACTCCCCGCCCTCATCAGCCTCGGGCGTTACATGCCCACCAAGCGGAGGTGTTACACATAGCTATGACCATAATCCTCAAATAAAGGAGTGCCAAGGTCCGAATGGCCTACACTACTCCCATATTTAGAAATCAACAACTCCTTCCAAAGGTTTCATATTATCAGTCAGCAAATGACACTTCCACTTTGCTAACATGCTATGTTACTCTTGCTATAATATTTTATTGTTACAGAAAATGGTAGGAGAGTAGGAGTTCACTTTAGTATATTTTTAGGCAATAATAATAGAGCAAGACAAACTTATTCATGGTCGTCTCTAATATTTTGGATGTTATATGTGAACTAAAATATAATGTCCTATTGATTATTTGAATAATTTTATTTTATGTGTTTCAACTTCAGAATCATTAATAACTCGTTCATATTCAAGGTTCTTTAAAAATTAGTTTTGATAGAAATTAGAGAAAAACTATATATACTTTGTGAAATAGTATTACCAATAAAATTTTAAAAACTTCAATTTGGAAAAACATCTTTCAGTTGTAGATGCAGCAATAACATAGATAGTTAATATTATTCTTTAAGTCATGCATGTATTAGGGAAATAATTGAGGTTAAACAGTTATTTTGGTCTTTAAATATGTTAAGTAGTGCCATTATAGTCCTCAAATATATTGAGATCAGAAACCATCCATGAATGTGCCTTCTATTATTAATTTCATTGACGAAACTCACTAACAAAATACACATTCAAATATGTTTTTGTAATTTATATACATTTAAAGATTATCGTCACTCAAGGATCAAAATGATTATTTACTCAATAATTAAATATCAAATTACTAAGCGAGTTGAGTTCTATTGGGAAAAATTTAAGTCCTACATGGACAAAATAGGACTTTTGGCAAGAGTGTATAATGTGGCACCCCTCACCTTACAAGACGGTTTTGTAAGTTTGGGTTATGCACGATTTAAAAATTTAATATGGTATTAGAGCATATCGATTGTGTCACCCACCATTTATATGCACGCTTCAAACCCAATAACGTTGGAAGTGAAGGGGTGTATCGGAAAAAACCATTAGGACTTGCACACACGCTAGACATGATTAGGCTCACCACTAAACTCATTACAAAATAAAATAAGAAATTATAGTGCTTAGTAAACATTAGAATAAGAAAAAAATAGTCCCCGTGAACCTAGCTCAATTGGTAGAGATAATGAATAATATATGTAGGGGCCGAGGTTCGAACTCCGGACACCCCACTTCTCCACATTTAAAATGTGTGATCTTCAACCACTAGGTTACTTGGCAAAAACAAAAAACAAAATAAGATAAAATAGAATACACGTAGTTGTTTGTTTTCTGTAATAAAATCTCATATGGTAAAATGATGTATGTACATTTGATAGGGTGAGAGAAGTGGGGTCTCTAAAGCTCATTACATATTAAATCCTTAATAATACAAAAATATTTAACTAAACATAGTATTGGTGTTCCTTGAAAATTAGGACTCACACACCCTAAATATGATTAGGGTTGCCACTAAACTCATTACAACATGAAAGAAGAAATTATAGTGCTTATAGTAAAGATATTAGAATAAGATAATATTGAATACACATAGTTGTTTGTTTTTCATTAGTGCAACTCAGATGATAAAATGATGTATATAATTTGATATGTTGAAACGCGAGTTCGAATCTATAATTTCTCACCTCTCTATACTTAGTGTGCGTGAGTTCTACTGTAAATCTCTTTGAAACAACAAAATAAAAGAAAATAAAAAACGTGTTTCTAATGTAAATGATTGCATTTTTGGTATGTTTCTAGAAGGTAGTCTTTGTGTTTGTGTTTTTTTGTGTGTGTTCAATATAAACCTCTAAAAAATAGTTTAATGATGACCTTTACCATTTTTAATAAAAAAAAGAATTTTATAATAATAAAAAATAAAGAATAAAGAAATATTTTATTAAACTTTAGTAAAAATGGTAGGGTTCATCTTTGGATCAAGCCCATATTTGACAACCCCTTATTTTCTATAAGTAGCTTAAGAATCATAGTGAAATGTGTTTGTAAAAAAGGCTGAAATCCACATTCTGATTTTATTCATTACTGAAAATTGTTTATATACAAGAGGATAACAAACTCATCCTAATATAGGCCAAATACAACTTCTAGTCTTTTAAGTTTGATATTTTTAACAAGTTAGTTTTTTAAGTATTATTTGTGACTAGTTTTCTATAGCTAAACTGTGATATGTTCAATAATGGTAGACTTTTCAAGACTATATTGGAGAATATGCATGTCAATTTTTAGCCCATACTCATAATTTATTCTTGTTCTCATTTCACTCAAATTGAATTGAATTCCCACGTTAACTATATTATTCTTTTGGTTATACATCAGCAGTTATAAGTTCAAGGAAAGTGGTACAGGCTTATAAATATCATTTTTATGTTACAAGCTTATAAAAGTCATTCTCATATGTTACTTATCTGTCAAAAAAATTGGTAGCATTACTTTTAATTTTATAGAAGATATTAGAAGTTACATATGACAATGACATATGATGGGTCACATTTGTTAACATGATCTTTGTTCCCTGATATTAATGACTGGCAGTAATGGAACATGGACACAAGGTAGTTCTGGAAATCATTTTGAGGAACTTAGCACACAAGAAATCCTATCAACTCACCACATTGGAAAGCTTCCTCAGGTATAAACCCTAATCCTCACTTATTCTATACTAAAAATAGCTAGGAAATGTAATTAATTTGTCAAATAAATGCATGCCTTCAAAAATAAAAACATATTGACCTTTTAAAAAAGATAATTTCAAATTTGGACATTTGAGATTTACTTGGTACAACATATTAATATAAAGTAAGTCAGTACATCCTCAAGGTCTCGATTCTTCCTAATGCTAATTTGAGTGAGTTAGTTTAGCTTTTACCAACAAAAATAATAATAATATAGCAAGTCAGTTCATCATTAGATCAGGACCATGTTAGAGAGATAGGCTCCCACATGAATTAATCTTTAGATATTGCAAAATATTGGACATTCTAATATAAACCTAAAAAACAGTCTAAAGTGAACCTTTACTATTTTTAGTAAATTTAAAAATGGGCAAATGATGTAAAATTTTATTAAAAATGACAAGGTCTAACTTTAGACCATTTTTTTAGGTTCATACTTATTCGGAGAAATAATGATTTTTCAACATCAAAATTTTAACGTCAAATATGGTTGTATCCTTTGACTTTGGTGTGTTTATTTGAAAATAATATATGTTGTAGAAGATGAAAAGTAAATGAAAGATTTCGATATAAAATATACCATATCATATATGATGTTTGTATTTTGATGTTCACGAAATGACACAACTGTTTACTTGTGCAGGGAAGCAGTTGTATACAATCAAGAAGTTCCAAGGATCAAAACATAGACTGCCCGAATCCAAACTTGGAGTTCACCTTAGGGAGATAAAATTAATTTTAGTTATTTACATGATTCATGGATCTCTCTACTAGCCATAGAAAAAAAAAATAGTGCACAGATATATATAAATAGCTAGTTAGGTAATGCTTTTGTGAAGACGAAGATGTCACTTTCAACACATCCAAGAAACTTTGTATATGTTTTTTTTAGCCAAAGATTATTAGTTGGTGATATATAAAGTTTTATTGTTAAAAGTAAAAGTTGAAAAAAGAAGGAATCAAATGTATTATAATGCTTTTGTTAATTGTGTCCTTTATTTACATTTTTCTTCATTTTCCCTAGGTTCCTACTGATTTGTCCATATATGTATAA
Protein sequence

>MS.gene27440.t1

MRAPRMRWTSSLHNRFVHAVELLGGHERATPKSVLELMDVKDLTLAHVKSHLQMYRTVKNTDKPAASSDGDENFMSLTQPHDQNKNYNASLDHDIGYTSNILWANSSSSNGTWTQGSSGNHFEELSTQEILSTHHIGKLPQVPTDLSIYV