Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27448.t1 | XP_013449239.1 | 94 | 349 | 11 | 1 | 1 | 339 | 1 | 349 | 1.00E-125 | 459.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27448.t1 | O04351 | 40.6 | 340 | 175 | 9 | 1 | 336 | 1 | 317 | 7.6e-21 | 102.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27448.t1 | A0A072U1F0 | 94.0 | 349 | 11 | 1 | 1 | 339 | 1 | 349 | 7.3e-126 | 459.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene27448.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27448.t1 | MTR_7g072630 | 93.123 | 349 | 14 | 1 | 1 | 339 | 1 | 349 | 0.0 | 618 |
| MS.gene27448.t1 | MTR_7g072875 | 92.701 | 274 | 10 | 1 | 76 | 339 | 14 | 287 | 0.0 | 501 |
| MS.gene27448.t1 | MTR_4g028930 | 49.140 | 407 | 110 | 16 | 1 | 337 | 1 | 380 | 9.24e-91 | 276 |
| MS.gene27448.t1 | MTR_5g075210 | 39.617 | 366 | 170 | 11 | 1 | 336 | 1 | 345 | 6.87e-62 | 201 |
| MS.gene27448.t1 | MTR_2g018030 | 60.606 | 66 | 26 | 0 | 271 | 336 | 277 | 342 | 2.67e-25 | 104 |
| MS.gene27448.t1 | MTR_8g087530 | 57.576 | 66 | 28 | 0 | 271 | 336 | 258 | 323 | 3.02e-22 | 95.9 |
| MS.gene27448.t1 | MTR_8g103520 | 53.226 | 62 | 29 | 0 | 271 | 332 | 224 | 285 | 1.97e-16 | 79.0 |
| MS.gene27448.t1 | MTR_0907s0020 | 44.928 | 69 | 38 | 0 | 264 | 332 | 95 | 163 | 1.51e-14 | 71.2 |
| MS.gene27448.t1 | MTR_4g052040 | 45.070 | 71 | 39 | 0 | 262 | 332 | 90 | 160 | 7.22e-14 | 69.3 |
| MS.gene27448.t1 | MTR_4g132740 | 47.619 | 63 | 33 | 0 | 273 | 335 | 83 | 145 | 4.20e-13 | 66.6 |
| MS.gene27448.t1 | MTR_3g111780 | 42.500 | 80 | 46 | 0 | 253 | 332 | 180 | 259 | 1.86e-12 | 67.4 |
| MS.gene27448.t1 | MTR_7g105790 | 44.068 | 59 | 33 | 0 | 274 | 332 | 113 | 171 | 3.18e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27448.t1 | AT1G06920 | 33.803 | 355 | 171 | 13 | 1 | 337 | 1 | 309 | 3.68e-40 | 144 |
| MS.gene27448.t1 | AT2G30400 | 32.973 | 370 | 161 | 11 | 1 | 336 | 1 | 317 | 5.42e-40 | 143 |
| MS.gene27448.t1 | AT5G58360 | 30.791 | 354 | 158 | 11 | 1 | 336 | 5 | 289 | 9.50e-35 | 129 |
| MS.gene27448.t1 | AT5G01840 | 33.824 | 340 | 142 | 10 | 1 | 335 | 1 | 262 | 1.37e-34 | 128 |
| MS.gene27448.t1 | AT4G18830 | 39.450 | 109 | 58 | 1 | 235 | 335 | 238 | 346 | 1.64e-19 | 88.6 |
| MS.gene27448.t1 | AT5G19650 | 50.000 | 66 | 33 | 0 | 270 | 335 | 153 | 218 | 8.53e-16 | 75.9 |
| MS.gene27448.t1 | AT3G52525 | 46.032 | 63 | 34 | 0 | 272 | 334 | 67 | 129 | 2.29e-13 | 67.4 |
| MS.gene27448.t1 | AT2G18500 | 41.791 | 67 | 39 | 0 | 269 | 335 | 224 | 290 | 3.95e-13 | 69.7 |
| MS.gene27448.t1 | AT2G36026 | 48.214 | 56 | 29 | 0 | 277 | 332 | 92 | 147 | 5.65e-12 | 64.3 |
Find 39 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACGGTCTGTTCTGATTTC+TGG | 0.132362 | 7.3:+32201338 | None:intergenic |
| AATGATGAACTCAACGTTAA+AGG | 0.223254 | 7.3:-32201113 | MS.gene27448:CDS |
| TCAAAGTGTTCAAGCAAATA+TGG | 0.233458 | 7.3:-32200728 | MS.gene27448:CDS |
| TTCTTCCTTTGTTGTGTTGT+TGG | 0.243928 | 7.3:+32201611 | None:intergenic |
| AGAATTGTTCTACTGATTAT+CGG | 0.312127 | 7.3:+32200695 | None:intergenic |
| CATTAGAACATCAAAGGATT+TGG | 0.325611 | 7.3:-32200808 | MS.gene27448:CDS |
| TGTAGATTCTATTGCTTGTT+TGG | 0.346884 | 7.3:+32201489 | None:intergenic |
| TTTGTTGTGTTGTTGGTGTT+TGG | 0.367246 | 7.3:+32201618 | None:intergenic |
| GAGAGGTAATGGGAAATTAC+AGG | 0.389366 | 7.3:-32201709 | None:intergenic |
| TACGTCTTCCACCATCGTTT+CGG | 0.400519 | 7.3:-32200937 | MS.gene27448:CDS |
| ATGATGAACTCAACGTTAAA+GGG | 0.407057 | 7.3:-32201112 | MS.gene27448:CDS |
| CCATCGTTTCGGCTGGCAAC+CGG | 0.420330 | 7.3:-32200926 | MS.gene27448:CDS |
| GTTCACCAGGTGTGAAACTT+AGG | 0.443025 | 7.3:-32201013 | MS.gene27448:CDS |
| TCTGTTCTGATTTCTGGCTC+AGG | 0.447190 | 7.3:+32201344 | None:intergenic |
| TCTGGTTTGGTCACAATTGG+TGG | 0.452293 | 7.3:+32201203 | None:intergenic |
| TTTCTTGGATTGCTTTGGTA+GGG | 0.457667 | 7.3:+32201539 | None:intergenic |
| GGAGATGATGATGAAGTCTT+TGG | 0.474574 | 7.3:+32201410 | None:intergenic |
| CAGACATGATGCCTAATGCT+TGG | 0.484513 | 7.3:-32201676 | MS.gene27448:CDS |
| CTGTTCTGATTTCTGGCTCA+GGG | 0.485970 | 7.3:+32201345 | None:intergenic |
| AGCTTGTAAAACCAAGCATT+AGG | 0.493200 | 7.3:+32201665 | None:intergenic |
| TCGATAATGATATCGTTTGT+CGG | 0.505634 | 7.3:+32201308 | None:intergenic |
| CTAACAGAAAATCTTCTACT+TGG | 0.507243 | 7.3:+32201035 | None:intergenic |
| ATTCACCAAGAATTTCAAAC+CGG | 0.519789 | 7.3:-32200986 | MS.gene27448:CDS |
| TCTTCCACCATCGTTTCGGC+TGG | 0.529072 | 7.3:-32200933 | MS.gene27448:CDS |
| CTATCAAGTGGAGAATCAAA+TGG | 0.529685 | 7.3:+32201389 | None:intergenic |
| ACTCATCTGAATTCAAAGAA+AGG | 0.530280 | 7.3:+32200766 | None:intergenic |
| CTTCACAATTGCAAAACTAT+CGG | 0.538926 | 7.3:+32200893 | None:intergenic |
| GAACAACATTAGAACATCAA+AGG | 0.551741 | 7.3:-32200814 | MS.gene27448:CDS |
| GGAAAATTCAAACACATGGC+CGG | 0.552191 | 7.3:-32200965 | MS.gene27448:CDS |
| AACTATCGGAAAGACTTCTC+CGG | 0.559644 | 7.3:+32200907 | None:intergenic |
| AAACACCAACAACACAACAA+AGG | 0.563217 | 7.3:-32201616 | MS.gene27448:CDS |
| GTTGCCAGCCGAAACGATGG+TGG | 0.586748 | 7.3:+32200929 | None:intergenic |
| CCGGTTGCCAGCCGAAACGA+TGG | 0.591822 | 7.3:+32200926 | None:intergenic |
| GATTCTCTAAAATCTCCTTG+TGG | 0.610956 | 7.3:+32200858 | None:intergenic |
| AACCGGAAAATTCAAACACA+TGG | 0.611341 | 7.3:-32200969 | MS.gene27448:CDS |
| GATTCTGCAGAGCTATCAAG+TGG | 0.613063 | 7.3:+32201377 | None:intergenic |
| TTGATCCTAAGTTTCACACC+TGG | 0.631177 | 7.3:+32201008 | None:intergenic |
| AAGTCATCATTGAATCCACA+AGG | 0.674680 | 7.3:-32200873 | MS.gene27448:CDS |
| CGTTTGTCGGAAGAAGAACA+CGG | 0.768531 | 7.3:+32201321 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAAAGTCAAGATTTTGA+AGG | - | chr7.3:32201318-32201337 | MS.gene27448:CDS | 25.0% |
| !! | ATTTGATGAATTTTTGCTTC+TGG | + | chr7.3:32201275-32201294 | None:intergenic | 25.0% |
| !! | GTAAAGTAGTAAGATTTTCT+TGG | + | chr7.3:32200884-32200903 | None:intergenic | 25.0% |
| !! | TTTGATGAATTTTTGCTTCT+GGG | + | chr7.3:32201274-32201293 | None:intergenic | 25.0% |
| !!! | TGTTTTGATGATGATTTTCT+TGG | + | chr7.3:32200953-32200972 | None:intergenic | 25.0% |
| AATGATGAACTCAACGTTAA+AGG | - | chr7.3:32201292-32201311 | MS.gene27448:CDS | 30.0% | |
| ACTCATCTGAATTCAAAGAA+AGG | + | chr7.3:32201642-32201661 | None:intergenic | 30.0% | |
| ATGATGAACTCAACGTTAAA+GGG | - | chr7.3:32201293-32201312 | MS.gene27448:CDS | 30.0% | |
| ATTCACCAAGAATTTCAAAC+CGG | - | chr7.3:32201419-32201438 | MS.gene27448:CDS | 30.0% | |
| CATTAGAACATCAAAGGATT+TGG | - | chr7.3:32201597-32201616 | MS.gene27448:CDS | 30.0% | |
| CTAACAGAAAATCTTCTACT+TGG | + | chr7.3:32201373-32201392 | None:intergenic | 30.0% | |
| CTTCACAATTGCAAAACTAT+CGG | + | chr7.3:32201515-32201534 | None:intergenic | 30.0% | |
| GAACAACATTAGAACATCAA+AGG | - | chr7.3:32201591-32201610 | MS.gene27448:CDS | 30.0% | |
| TCGATAATGATATCGTTTGT+CGG | + | chr7.3:32201100-32201119 | None:intergenic | 30.0% | |
| TGTAGATTCTATTGCTTGTT+TGG | + | chr7.3:32200919-32200938 | None:intergenic | 30.0% | |
| ! | AACAAGTAGTTGAACTTTTC+TGG | + | chr7.3:32201223-32201242 | None:intergenic | 30.0% |
| ! | AAGATTTTCTTGGATTGCTT+TGG | + | chr7.3:32200874-32200893 | None:intergenic | 30.0% |
| ! | TCAAAGTGTTCAAGCAAATA+TGG | - | chr7.3:32201677-32201696 | MS.gene27448:CDS | 30.0% |
| ! | TTTTACAAGCTTAGAGACAT+GGG | - | chr7.3:32200752-32200771 | MS.gene27448:CDS | 30.0% |
| !! | AGATTTTAGAGAATCAATGG+TGG | - | chr7.3:32201555-32201574 | MS.gene27448:CDS | 30.0% |
| !! | AGGAGATTTTAGAGAATCAA+TGG | - | chr7.3:32201552-32201571 | MS.gene27448:CDS | 30.0% |
| !! | ATTTTCCGGTTTGAAATTCT+TGG | + | chr7.3:32201427-32201446 | None:intergenic | 30.0% |
| !!! | CTTTGTTTTTGATGTGTTTG+AGG | + | chr7.3:32200843-32200862 | None:intergenic | 30.0% |
| !!! | TTTGATGATGATTTTCTTGG+TGG | + | chr7.3:32200950-32200969 | None:intergenic | 30.0% |
| AAACACCAACAACACAACAA+AGG | - | chr7.3:32200789-32200808 | MS.gene27448:CDS | 35.0% | |
| AACCGGAAAATTCAAACACA+TGG | - | chr7.3:32201436-32201455 | MS.gene27448:CDS | 35.0% | |
| AAGTCATCATTGAATCCACA+AGG | - | chr7.3:32201532-32201551 | MS.gene27448:CDS | 35.0% | |
| AGCTTGTAAAACCAAGCATT+AGG | + | chr7.3:32200743-32200762 | None:intergenic | 35.0% | |
| CTATCAAGTGGAGAATCAAA+TGG | + | chr7.3:32201019-32201038 | None:intergenic | 35.0% | |
| TTTCTTGGATTGCTTTGGTA+GGG | + | chr7.3:32200869-32200888 | None:intergenic | 35.0% | |
| ! | AGATTTTCTGTTAGTTCACC+AGG | - | chr7.3:32201379-32201398 | MS.gene27448:CDS | 35.0% |
| ! | GTTTTACAAGCTTAGAGACA+TGG | - | chr7.3:32200751-32200770 | MS.gene27448:CDS | 35.0% |
| ! | TTCTTCCTTTGTTGTGTTGT+TGG | + | chr7.3:32200797-32200816 | None:intergenic | 35.0% |
| ! | TTTGTTGTGTTGTTGGTGTT+TGG | + | chr7.3:32200790-32200809 | None:intergenic | 35.0% |
| ! | TTTTCTTGGATTGCTTTGGT+AGG | + | chr7.3:32200870-32200889 | None:intergenic | 35.0% |
| !! | AGATTTTGAAGGAAGAACAG+AGG | - | chr7.3:32201329-32201348 | MS.gene27448:CDS | 35.0% |
| !! | GATTCTCTAAAATCTCCTTG+TGG | + | chr7.3:32201550-32201569 | None:intergenic | 35.0% |
| !!! | GTAGTTGAACTTTTCTGGTT+TGG | + | chr7.3:32201218-32201237 | None:intergenic | 35.0% |
| !!! | GTCTTTGGTGTTCTTCTTTT+TGG | + | chr7.3:32200983-32201002 | None:intergenic | 35.0% |
| !!! | TTTTCTGGTTTGGTCACAAT+TGG | + | chr7.3:32201208-32201227 | None:intergenic | 35.0% |
| AACTATCGGAAAGACTTCTC+CGG | + | chr7.3:32201501-32201520 | None:intergenic | 40.0% | |
| GGAAAATTCAAACACATGGC+CGG | - | chr7.3:32201440-32201459 | MS.gene27448:CDS | 40.0% | |
| TTGATCCTAAGTTTCACACC+TGG | + | chr7.3:32201400-32201419 | None:intergenic | 40.0% | |
| ! | GGAGATGATGATGAAGTCTT+TGG | + | chr7.3:32200998-32201017 | None:intergenic | 40.0% |
| ! | GGCCATGTGTTTGAATTTTC+CGG | + | chr7.3:32201441-32201460 | None:intergenic | 40.0% |
| ACACGGTCTGTTCTGATTTC+TGG | + | chr7.3:32201070-32201089 | None:intergenic | 45.0% | |
| CAGACATGATGCCTAATGCT+TGG | - | chr7.3:32200729-32200748 | MS.gene27448:CDS | 45.0% | |
| CGTTTGTCGGAAGAAGAACA+CGG | + | chr7.3:32201087-32201106 | None:intergenic | 45.0% | |
| GATTCTGCAGAGCTATCAAG+TGG | + | chr7.3:32201031-32201050 | None:intergenic | 45.0% | |
| GTTCACCAGGTGTGAAACTT+AGG | - | chr7.3:32201392-32201411 | MS.gene27448:CDS | 45.0% | |
| TACGTCTTCCACCATCGTTT+CGG | - | chr7.3:32201468-32201487 | MS.gene27448:CDS | 45.0% | |
| ! | TGGTGGAAGACGTACTTTTC+CGG | + | chr7.3:32201462-32201481 | None:intergenic | 45.0% |
| !! | CTGTTCTGATTTCTGGCTCA+GGG | + | chr7.3:32201063-32201082 | None:intergenic | 45.0% |
| !! | TCTGGTTTGGTCACAATTGG+TGG | + | chr7.3:32201205-32201224 | None:intergenic | 45.0% |
| !! | TCTGTTCTGATTTCTGGCTC+AGG | + | chr7.3:32201064-32201083 | None:intergenic | 45.0% |
| TCTTCCACCATCGTTTCGGC+TGG | - | chr7.3:32201472-32201491 | MS.gene27448:CDS | 55.0% | |
| CCATCGTTTCGGCTGGCAAC+CGG | - | chr7.3:32201479-32201498 | MS.gene27448:CDS | 60.0% | |
| GTTGCCAGCCGAAACGATGG+TGG | + | chr7.3:32201479-32201498 | None:intergenic | 60.0% | |
| CCGGTTGCCAGCCGAAACGA+TGG | + | chr7.3:32201482-32201501 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 32200704 | 32201723 | 32200704 | ID=MS.gene27448 |
| chr7.3 | mRNA | 32200704 | 32201723 | 32200704 | ID=MS.gene27448.t1;Parent=MS.gene27448 |
| chr7.3 | exon | 32200704 | 32201723 | 32200704 | ID=MS.gene27448.t1.exon1;Parent=MS.gene27448.t1 |
| chr7.3 | CDS | 32200704 | 32201723 | 32200704 | ID=cds.MS.gene27448.t1;Parent=MS.gene27448.t1 |
| Gene Sequence |
| Protein sequence |
