AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27799

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27799.t1 MTR_8g086410 95.620 274 5 3 1 270 111 381 0.0 536
MS.gene27799.t1 MTR_8g086410 95.620 274 5 3 1 270 109 379 0.0 536
MS.gene27799.t1 MTR_8g086410 93.238 281 5 4 1 270 111 388 0.0 529
MS.gene27799.t1 MTR_2g016220 44.369 293 85 10 1 270 89 326 1.80e-61 198
MS.gene27799.t1 MTR_2g016220 44.369 293 85 10 1 270 89 326 3.13e-61 198
MS.gene27799.t1 MTR_2g016220 43.771 297 85 11 1 270 89 330 3.97e-59 192
MS.gene27799.t1 MTR_2g084230 45.312 192 79 7 1 192 97 262 5.26e-36 130
MS.gene27799.t1 MTR_2g084230 45.312 192 79 7 1 192 97 262 1.31e-35 131
MS.gene27799.t1 MTR_7g069660 82.258 62 11 0 2 63 72 133 2.52e-30 116
MS.gene27799.t1 MTR_2g023580 73.333 60 16 0 1 60 55 114 1.73e-26 103
MS.gene27799.t1 MTR_0223s0040 59.494 79 31 1 1 79 18 95 4.68e-25 101
MS.gene27799.t1 MTR_1g112370 62.121 66 25 0 1 66 22 87 9.27e-23 95.9
MS.gene27799.t1 MTR_5g029470 58.667 75 30 1 2 75 220 294 1.36e-20 90.5
MS.gene27799.t1 MTR_6g071625 69.643 56 17 0 2 57 203 258 3.41e-20 89.4
MS.gene27799.t1 MTR_7g115530 54.667 75 32 1 2 76 219 291 3.68e-20 88.2
MS.gene27799.t1 MTR_1g021520 53.571 84 38 1 2 84 127 210 5.63e-20 87.4
MS.gene27799.t1 MTR_7g115530 54.667 75 32 1 2 76 219 291 8.09e-20 87.8
MS.gene27799.t1 MTR_7g115530 54.667 75 32 1 2 76 219 291 1.11e-19 88.2
MS.gene27799.t1 MTR_8g066310 60.294 68 22 1 2 64 174 241 1.56e-19 87.0
MS.gene27799.t1 MTR_7g098250 66.071 56 19 0 2 57 263 318 2.33e-19 87.4
MS.gene27799.t1 MTR_3g064840 69.643 56 17 0 2 57 275 330 3.57e-19 87.0
MS.gene27799.t1 MTR_3g450310 60.714 56 22 0 2 57 20 75 5.44e-19 83.2
MS.gene27799.t1 MTR_1g080330 64.286 56 20 0 2 57 176 231 6.72e-19 84.3
MS.gene27799.t1 MTR_7g115530 64.286 56 20 0 2 57 219 274 7.18e-19 85.1
MS.gene27799.t1 MTR_1g080330 64.286 56 20 0 2 57 176 231 9.60e-19 85.1
MS.gene27799.t1 MTR_7g093030 51.429 70 33 1 3 71 214 283 2.51e-18 83.2
MS.gene27799.t1 MTR_1g053835 54.286 70 31 1 3 71 260 329 4.23e-18 83.6
MS.gene27799.t1 MTR_7g093030 51.429 70 33 1 3 71 214 283 5.58e-18 83.2
MS.gene27799.t1 MTR_7g115530 62.500 56 21 0 2 57 219 274 1.98e-17 80.1
MS.gene27799.t1 MTR_7g115530 65.385 52 18 0 2 53 219 270 2.71e-17 80.1
MS.gene27799.t1 MTR_6g032990 62.264 53 20 0 2 54 43 95 5.90e-17 80.1
MS.gene27799.t1 MTR_1g053830 48.571 70 35 1 3 71 20 89 8.70e-17 74.3
MS.gene27799.t1 MTR_5g027440 62.264 53 20 0 2 54 26 78 1.56e-16 77.8
MS.gene27799.t1 MTR_2g086450 58.929 56 23 0 2 57 46 101 1.83e-16 78.6
MS.gene27799.t1 MTR_7g088070 63.636 55 20 0 3 57 52 106 4.03e-16 76.6
MS.gene27799.t1 MTR_7g088070 63.636 55 20 0 3 57 52 106 4.15e-16 76.6
MS.gene27799.t1 MTR_1g090670 56.452 62 25 1 2 63 45 104 7.75e-16 75.9
MS.gene27799.t1 MTR_2g027800 58.929 56 23 0 2 57 40 95 1.09e-15 75.9
MS.gene27799.t1 MTR_2g027800 60.377 53 21 0 2 54 40 92 1.67e-15 75.1
MS.gene27799.t1 MTR_2g027860 63.636 55 20 0 3 57 51 105 1.90e-15 75.5
MS.gene27799.t1 MTR_5g041350 51.786 56 27 0 2 57 16 71 4.27e-15 74.7
MS.gene27799.t1 MTR_6g444980 53.571 56 26 0 2 57 36 91 2.10e-14 72.8
MS.gene27799.t1 MTR_6g444980 53.571 56 26 0 2 57 36 91 2.44e-14 72.4
MS.gene27799.t1 MTR_1g053800 58.491 53 22 0 3 55 179 231 6.20e-14 70.9
MS.gene27799.t1 MTR_7g068600 59.322 59 21 1 3 58 15 73 1.05e-13 69.3
MS.gene27799.t1 MTR_4g081710 51.786 56 27 0 2 57 18 73 1.58e-13 69.7
MS.gene27799.t1 MTR_7g093010 60.377 53 21 0 3 55 215 267 6.10e-13 68.2
MS.gene27799.t1 MTR_4g086835 36.842 95 52 1 3 97 276 362 2.20e-11 63.9
MS.gene27799.t1 MTR_4g021790 51.923 52 24 1 3 54 170 220 3.49e-11 62.4
MS.gene27799.t1 MTR_4g113140 53.846 52 24 0 3 54 237 288 4.75e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27799.t1 AT2G38300 79.688 64 13 0 1 64 54 117 2.34e-30 116
MS.gene27799.t1 AT2G42660 81.356 59 11 0 1 59 50 108 8.75e-28 107
MS.gene27799.t1 AT2G40260 84.746 59 9 0 2 60 83 141 1.73e-27 110
MS.gene27799.t1 AT4G04605 60.759 79 29 1 1 77 14 92 8.92e-27 104
MS.gene27799.t1 AT2G02060 76.364 55 13 0 1 55 29 83 3.41e-24 98.2
MS.gene27799.t1 AT2G02060 76.364 55 13 0 1 55 29 83 3.41e-24 98.2
MS.gene27799.t1 AT1G14600 65.079 63 22 0 1 63 23 85 1.02e-23 97.1
MS.gene27799.t1 AT1G32240 60.000 75 29 1 2 75 214 288 3.22e-21 92.0
MS.gene27799.t1 AT1G32240 60.000 75 29 1 2 75 214 288 5.78e-21 91.7
MS.gene27799.t1 AT5G42630 65.574 61 21 0 2 62 106 166 5.87e-21 89.0
MS.gene27799.t1 AT5G42630 65.574 61 21 0 2 62 106 166 1.26e-20 89.0
MS.gene27799.t1 AT5G16560 54.762 84 37 1 2 84 220 303 2.16e-20 90.1
MS.gene27799.t1 AT4G17695 58.108 74 31 0 2 75 165 238 5.52e-20 88.2
MS.gene27799.t1 AT4G04580 60.000 60 24 0 2 61 15 74 1.39e-19 83.6
MS.gene27799.t1 AT4G04555 57.377 61 26 0 1 61 14 74 4.91e-18 77.8
MS.gene27799.t1 AT5G45580 40.187 107 52 2 2 107 8 103 7.46e-18 81.3
MS.gene27799.t1 AT4G28610 63.636 55 20 0 3 57 227 281 9.88e-18 82.4
MS.gene27799.t1 AT5G45580 40.187 107 52 2 2 107 24 119 1.04e-17 81.3
MS.gene27799.t1 AT5G29000 63.636 55 20 0 3 57 233 287 1.96e-17 80.9
MS.gene27799.t1 AT3G04450 60.000 55 22 0 3 57 240 294 1.98e-17 80.9
MS.gene27799.t1 AT5G29000 63.636 55 20 0 3 57 190 244 2.57e-17 81.3
MS.gene27799.t1 AT5G29000 63.636 55 20 0 3 57 190 244 2.57e-17 81.3
MS.gene27799.t1 AT5G29000 63.636 55 20 0 3 57 233 287 3.00e-17 81.3
MS.gene27799.t1 AT3G04450 60.000 55 22 0 3 57 240 294 3.25e-17 81.3
MS.gene27799.t1 AT3G04450 60.000 55 22 0 3 57 200 254 3.46e-17 80.9
MS.gene27799.t1 AT3G04450 60.000 55 22 0 3 57 200 254 3.46e-17 80.9
MS.gene27799.t1 AT3G13040 65.455 55 19 0 3 57 243 297 1.28e-16 79.3
MS.gene27799.t1 AT3G13040 65.455 55 19 0 3 57 243 297 1.28e-16 79.3
MS.gene27799.t1 AT3G13040 65.455 55 19 0 3 57 243 297 1.28e-16 79.3
MS.gene27799.t1 AT3G04030 55.357 56 25 0 2 57 46 101 3.42e-16 78.2
MS.gene27799.t1 AT3G04030 55.357 56 25 0 2 57 46 101 3.94e-16 77.8
MS.gene27799.t1 AT5G18240 48.571 70 35 1 2 70 46 115 5.50e-16 77.4
MS.gene27799.t1 AT5G18240 48.571 70 35 1 2 70 46 115 5.50e-16 77.4
MS.gene27799.t1 AT5G18240 48.571 70 35 1 2 70 46 115 6.33e-16 77.4
MS.gene27799.t1 AT5G18240 48.571 70 35 1 2 70 46 115 6.33e-16 77.4
MS.gene27799.t1 AT5G18240 48.571 70 35 1 2 70 46 115 6.65e-16 77.4
MS.gene27799.t1 AT2G01060 63.636 55 20 0 3 57 17 71 1.07e-15 75.5
MS.gene27799.t1 AT2G20400 56.944 72 26 2 3 70 233 303 1.30e-15 76.3
MS.gene27799.t1 AT5G06800 58.182 55 23 0 3 57 194 248 1.48e-15 75.1
MS.gene27799.t1 AT3G12730 55.357 56 25 0 2 57 24 79 2.09e-15 73.9
MS.gene27799.t1 AT2G20400 61.818 55 21 0 3 57 233 287 3.01e-15 75.5
MS.gene27799.t1 AT3G24120 48.529 68 33 1 2 69 42 107 4.43e-15 73.9
MS.gene27799.t1 AT3G24120 48.529 68 33 1 2 69 42 107 4.43e-15 73.9
MS.gene27799.t1 AT5G06800 58.182 55 23 0 3 57 194 248 5.64e-15 74.3
MS.gene27799.t1 AT5G06800 58.182 55 23 0 3 57 194 248 5.66e-15 74.3
MS.gene27799.t1 AT2G06020 43.956 91 42 1 3 84 88 178 7.64e-15 73.6
MS.gene27799.t1 AT1G69580 53.226 62 26 1 2 60 35 96 8.41e-15 73.6
MS.gene27799.t1 AT1G69580 55.357 56 25 0 2 57 35 90 8.72e-15 73.6
MS.gene27799.t1 AT4G13640 54.717 53 24 0 2 54 38 90 1.06e-14 72.8
MS.gene27799.t1 AT4G13640 54.717 53 24 0 2 54 38 90 1.09e-14 72.8
MS.gene27799.t1 AT1G79430 53.571 56 26 0 2 57 35 90 1.49e-14 73.2
MS.gene27799.t1 AT3G04030 53.571 56 25 1 2 57 46 100 1.38e-13 70.5
MS.gene27799.t1 AT3G62670 52.632 57 23 1 2 54 212 268 7.28e-12 65.5
MS.gene27799.t1 AT2G03500 53.846 52 24 0 3 54 235 286 3.79e-11 63.2
MS.gene27799.t1 AT1G25550 53.846 52 24 0 3 54 211 262 6.59e-11 62.4

Find 67 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAGTTGAAGCACCAATT+TGG 0.203737 4.3:+14270713 None:intergenic
GATGATTTAAGTACCATTCT+TGG 0.272460 4.3:+14268926 None:intergenic
TGATCTTCCTTGTGTATTAA+TGG 0.279777 4.3:+14268852 None:intergenic
ATGATTGTTCATGATATCTA+AGG 0.331312 4.3:+14268726 None:intergenic
ATCAGTTGAAGCACCAATTT+GGG 0.338016 4.3:+14270714 None:intergenic
TATAGTTTACCTTGAATTGA+TGG 0.341831 4.3:+14269026 None:intergenic
GAACCAGCAGCTTCAAGTTA+AGG 0.347972 4.3:-14268818 MS.gene27799:CDS
TGCAGGAGCAACACCCAAAT+TGG 0.368712 4.3:-14270727 MS.gene27799:intron
CACAGATCAACCCATGAATT+AGG 0.371135 4.3:-14269213 MS.gene27799:CDS
TCATGAACAATCATCCATAT+TGG 0.374740 4.3:-14268717 MS.gene27799:CDS
AAGCTGCTGGTTCAAAGTTA+TGG 0.381198 4.3:+14268828 None:intergenic
AGAAATTGGCTTGGTCTCAT+AGG 0.398094 4.3:+14268769 None:intergenic
GGTACTTAAATCATCAATCT+TGG 0.408196 4.3:-14268918 MS.gene27799:CDS
TCATGTCAAGAGTCACTTGC+AGG 0.410009 4.3:-14270667 MS.gene27799:intron
GTGATAAGGATGAGGGATGC+AGG 0.412034 4.3:+14269070 None:intergenic
ATGTTACCTTAATCCGAAAC+GGG 0.413271 4.3:+14268533 None:intergenic
ATATAATATACTTACTCTCT+TGG 0.424695 4.3:+14270867 None:intergenic
TTCTCTTCAAGAAATTGGCT+TGG 0.425569 4.3:+14268760 None:intergenic
AGACCCGCTGAGGTGAGCTT+TGG 0.428693 4.3:-14268640 MS.gene27799:CDS
GAGAAATGGAACCTAATTCA+TGG 0.443905 4.3:+14269202 None:intergenic
TGTGTATTAATGGCTGTTGA+TGG 0.444504 4.3:+14268862 None:intergenic
AGCAAGAAACTTGACGAGGC+TGG 0.448557 4.3:-14269982 MS.gene27799:CDS
TTTCCAAAGCTCACCTCAGC+GGG 0.450601 4.3:+14268637 None:intergenic
ATAAAAGAGTGATGAAGATC+AGG 0.470459 4.3:+14270914 None:intergenic
ATGGGCTACATGGTGAGAAA+TGG 0.471248 4.3:+14269188 None:intergenic
AATGTTACCTTAATCCGAAA+CGG 0.472630 4.3:+14268532 None:intergenic
CAACATTTCAAGATGGCAAA+TGG 0.480821 4.3:-14269147 MS.gene27799:CDS
CCTCGTCAAGTTTCTTGCTC+CGG 0.482077 4.3:+14269986 None:intergenic
TATCCTTAACTTGAAGCTGC+TGG 0.484791 4.3:+14268815 None:intergenic
ATTCAAAGCCCATCAATTCA+AGG 0.485522 4.3:-14269035 MS.gene27799:intron
ATAGTTTACCTTGAATTGAT+GGG 0.489818 4.3:+14269027 None:intergenic
GATCAAAGATGCCTAGGCTT+AGG 0.490445 4.3:-14270943 None:intergenic
ATGCATGCTGTTGAAAGGCT+TGG 0.493618 4.3:-14270893 MS.gene27799:CDS
AATTCATTGCTCATGTAGCT+TGG 0.505208 4.3:+14268892 None:intergenic
AATTTCTTGAAGAGAAAAGA+TGG 0.506639 4.3:-14268753 MS.gene27799:CDS
TCATGGGTTGATCTGTGTGT+TGG 0.507129 4.3:+14269219 None:intergenic
CAAAGATGCCTAGGCTTAGG+TGG 0.512420 4.3:-14270940 None:intergenic
TTGAACTATTAAAATAGTTG+TGG 0.532058 4.3:+14268572 None:intergenic
TTGGAAACAACACAAGAAGC+AGG 0.535242 4.3:-14268621 MS.gene27799:CDS
ATGTTAGATCAAAGATGCCT+AGG 0.555843 4.3:-14270949 None:intergenic
ATGTGAATGTGATAAGGATG+AGG 0.556397 4.3:+14269062 None:intergenic
ATGTGAATGTGAATGTGATA+AGG 0.557968 4.3:+14269056 None:intergenic
CATGCTGTTGAAAGGCTTGG+AGG 0.559982 4.3:-14270890 MS.gene27799:CDS
GATGAGGTATCATGGACTGA+TGG 0.560341 4.3:+14269169 None:intergenic
GAAATGTTGATGAGGTATCA+TGG 0.573003 4.3:+14269161 None:intergenic
TGTGGAAATTATAACTGTGC+TGG 0.573367 4.3:+14269097 None:intergenic
AGAAATGGAACCTAATTCAT+GGG 0.583302 4.3:+14269203 None:intergenic
TCATGGACTGATGGGCTACA+TGG 0.586690 4.3:+14269178 None:intergenic
TCAGGAGTCCACCTAAGCCT+AGG 0.589432 4.3:+14270932 None:intergenic
ACCTCATCAACATTTCAAGA+TGG 0.592000 4.3:-14269154 MS.gene27799:CDS
ATGAGGGATGCAGGAGACTG+TGG 0.594666 4.3:+14269079 None:intergenic
CATTTCAAGATGGCAAATGG+TGG 0.597539 4.3:-14269144 MS.gene27799:CDS
TGTGAATGTGATAAGGATGA+GGG 0.599304 4.3:+14269063 None:intergenic
ATGAGGTATCATGGACTGAT+GGG 0.611244 4.3:+14269170 None:intergenic
TGATTGTTCATGATATCTAA+GGG 0.612865 4.3:+14268727 None:intergenic
TGGTCTCATAGGAGCAAGTG+TGG 0.621257 4.3:+14268780 None:intergenic
GCCATCTTGAAATGTTGATG+AGG 0.640047 4.3:+14269153 None:intergenic
GTTTCCAAAGCTCACCTCAG+CGG 0.640457 4.3:+14268636 None:intergenic
AACTTGACGAGGCTGGACAA+AGG 0.644613 4.3:-14269975 MS.gene27799:intron
TGGAAACAACACAAGAAGCA+GGG 0.655021 4.3:-14268620 MS.gene27799:CDS
CTTCAACTGATGAATGTGAG+AGG 0.660411 4.3:-14270702 MS.gene27799:CDS
TGTTACCTTAATCCGAAACG+GGG 0.678201 4.3:+14268534 None:intergenic
AACATCACTTAGACCCGCTG+AGG 0.687013 4.3:-14268650 MS.gene27799:CDS
TCAACAGCCATTAATACACA+AGG 0.693154 4.3:-14268859 MS.gene27799:CDS
CCGGAGCAAGAAACTTGACG+AGG 0.718472 4.3:-14269986 MS.gene27799:CDS
GTTACCTTAATCCGAAACGG+GGG 0.718704 4.3:+14268535 None:intergenic
TTGTTCATGATATCTAAGGG+AGG 0.723510 4.3:+14268730 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATATATTAAAATAAAA+GGG - chr4.3:14268933-14268952 MS.gene27799:intron 0.0%
!! AAATATATTAAAATAAAAGG+GGG - chr4.3:14268935-14268954 MS.gene27799:intron 10.0%
!!! AGAATTTTTTTTTCATTATA+GGG + chr4.3:14269577-14269596 None:intergenic 10.0%
!!! TAGAATTTTTTTTTCATTAT+AGG + chr4.3:14269578-14269597 None:intergenic 10.0%
!! AATATATTAAAATAAAAGGG+GGG - chr4.3:14268936-14268955 MS.gene27799:intron 15.0%
!! AATGTCAAAATAAACAAAAT+CGG + chr4.3:14270204-14270223 None:intergenic 15.0%
!! AATTGTGAAATTGTTTAATT+TGG - chr4.3:14268676-14268695 MS.gene27799:CDS 15.0%
!! ATATAACAGTGAAAAAAATA+TGG + chr4.3:14269846-14269865 None:intergenic 15.0%
!! TCTAATTAAAAATTTGTACT+GGG - chr4.3:14270126-14270145 MS.gene27799:intron 15.0%
!!! AAAGTTACATTTTTTTACTT+AGG - chr4.3:14270524-14270543 MS.gene27799:intron 15.0%
!!! GTATTTCATAAATCATTTTA+TGG - chr4.3:14269442-14269461 MS.gene27799:intron 15.0%
!!! TTTTCCTTGATTTTTTTTTT+TGG - chr4.3:14269360-14269379 MS.gene27799:intron 15.0%
!! AAACAATTTCACAATTATCT+AGG + chr4.3:14268672-14268691 None:intergenic 20.0%
!! AACAATTTCACAATTATCTA+GGG + chr4.3:14268671-14268690 None:intergenic 20.0%
!! AATAAATACAATAGATGGTT+GGG - chr4.3:14269247-14269266 MS.gene27799:intron 20.0%
!! ATATAATATACTTACTCTCT+TGG + chr4.3:14268611-14268630 None:intergenic 20.0%
!! ATATATCAACACTTAATTTC+AGG + chr4.3:14269422-14269441 None:intergenic 20.0%
!! CACTGTTATATAAAAACAAA+TGG - chr4.3:14269855-14269874 MS.gene27799:intron 20.0%
!! GATTTATAAGACAAAAAAAC+AGG + chr4.3:14269720-14269739 None:intergenic 20.0%
!! GTCTAATTAAAAATTTGTAC+TGG - chr4.3:14270125-14270144 MS.gene27799:intron 20.0%
!! TAGTAATTACATAAAAGAGT+AGG - chr4.3:14270103-14270122 MS.gene27799:intron 20.0%
!! TTGAACTATTAAAATAGTTG+TGG + chr4.3:14270906-14270925 None:intergenic 20.0%
!!! AATCAAAGTATTACAATACA+AGG - chr4.3:14270156-14270175 MS.gene27799:intron 20.0%
!!! ATCAAAGTATTACAATACAA+GGG - chr4.3:14270157-14270176 MS.gene27799:intron 20.0%
!!! GTCATCTATTTTAAAATTGA+TGG - chr4.3:14270009-14270028 MS.gene27799:intron 20.0%
!!! TGTAAATTTTTCTTCCAATA+TGG + chr4.3:14270775-14270794 None:intergenic 20.0%
!!! TTATTTTTTTTTCTCTCTAG+TGG + chr4.3:14269215-14269234 None:intergenic 20.0%
!!! TTTATTTTTTTCCATTCAAG+TGG - chr4.3:14270497-14270516 MS.gene27799:intron 20.0%
! AAAGAGAAAGAGAAAAAGTA+TGG + chr4.3:14269636-14269655 None:intergenic 25.0%
! AAATACCGAATGATTTATCT+TGG - chr4.3:14269141-14269160 MS.gene27799:CDS 25.0%
! AAGTAAGTATCATTAGAGTT+GGG + chr4.3:14270055-14270074 None:intergenic 25.0%
! AATACCGAATGATTTATCTT+GGG - chr4.3:14269142-14269161 MS.gene27799:CDS 25.0%
! AATTCCCAAGATAAATCATT+CGG + chr4.3:14269149-14269168 None:intergenic 25.0%
! AATTTCTTGAAGAGAAAAGA+TGG - chr4.3:14270722-14270741 MS.gene27799:CDS 25.0%
! ACCACCAAAAAAAAAAATCA+AGG + chr4.3:14269367-14269386 None:intergenic 25.0%
! AGGTATATAAGACAAAATTG+AGG - chr4.3:14268828-14268847 MS.gene27799:CDS 25.0%
! ATAAATACAATAGATGGTTG+GGG - chr4.3:14269248-14269267 MS.gene27799:intron 25.0%
! ATAGTTTACCTTGAATTGAT+GGG + chr4.3:14270451-14270470 None:intergenic 25.0%
! ATGATTGTTCATGATATCTA+AGG + chr4.3:14270752-14270771 None:intergenic 25.0%
! CAATAAATACAATAGATGGT+TGG - chr4.3:14269246-14269265 MS.gene27799:intron 25.0%
! GATAGAAAAATATGAAGTGT+TGG - chr4.3:14269289-14269308 MS.gene27799:intron 25.0%
! GATGCAATAAATACAATAGA+TGG - chr4.3:14269242-14269261 MS.gene27799:intron 25.0%
! GTTGTGTTCATTATTAATGT+TGG - chr4.3:14268718-14268737 MS.gene27799:CDS 25.0%
! TATAGTTTACCTTGAATTGA+TGG + chr4.3:14270452-14270471 None:intergenic 25.0%
! TCTAGTGGTATATTATTCTT+TGG + chr4.3:14269200-14269219 None:intergenic 25.0%
! TGATTGTTCATGATATCTAA+GGG + chr4.3:14270751-14270770 None:intergenic 25.0%
!! AGATCAATTAATTTTTCTGC+AGG - chr4.3:14268980-14268999 MS.gene27799:intron 25.0%
!! ATCTTTTCTCTTCAAGAAAT+TGG + chr4.3:14270723-14270742 None:intergenic 25.0%
!! GATCAATTAATTTTTCTGCA+GGG - chr4.3:14268981-14269000 MS.gene27799:intron 25.0%
!! TTCAAATAAGTGACATCTTT+TGG - chr4.3:14269789-14269808 MS.gene27799:intron 25.0%
!!! ATTTTTTTTTTTGGTGGTGA+TGG - chr4.3:14269369-14269388 MS.gene27799:intron 25.0%
!!! TCCTTGATTTTTTTTTTTGG+TGG - chr4.3:14269363-14269382 MS.gene27799:intron 25.0%
AATTGTGCACATGTGTAAAA+CGG - chr4.3:14269978-14269997 MS.gene27799:CDS 30.0%
ACGTGAAACAAAAATATCAG+TGG + chr4.3:14268914-14268933 None:intergenic 30.0%
AGAAATGGAACCTAATTCAT+GGG + chr4.3:14270275-14270294 None:intergenic 30.0%
ATAAAAGAGTGATGAAGATC+AGG + chr4.3:14268564-14268583 None:intergenic 30.0%
ATGTGAATGTGAATGTGATA+AGG + chr4.3:14270422-14270441 None:intergenic 30.0%
GAAGTAAGTATCATTAGAGT+TGG + chr4.3:14270056-14270075 None:intergenic 30.0%
GAATGATTTATCTTGGGAAT+TGG - chr4.3:14269148-14269167 MS.gene27799:CDS 30.0%
GATGATTTAAGTACCATTCT+TGG + chr4.3:14270552-14270571 None:intergenic 30.0%
GGCAGACAAAAAAAAATTCA+AGG + chr4.3:14269556-14269575 None:intergenic 30.0%
GGTACTTAAATCATCAATCT+TGG - chr4.3:14270557-14270576 MS.gene27799:intron 30.0%
GTGTTCATTATTAATGTTGG+TGG - chr4.3:14268721-14268740 MS.gene27799:CDS 30.0%
TATTACAATACAAGGGCTTA+GGG - chr4.3:14270164-14270183 MS.gene27799:intron 30.0%
TCATGAACAATCATCCATAT+TGG - chr4.3:14270758-14270777 MS.gene27799:intron 30.0%
TGATCTTCCTTGTGTATTAA+TGG + chr4.3:14270626-14270645 None:intergenic 30.0%
! CAATTTTCATTCAGATGTAC+CGG - chr4.3:14269470-14269489 MS.gene27799:intron 30.0%
! TAACTTTAATGCCACTTGAA+TGG + chr4.3:14270511-14270530 None:intergenic 30.0%
!! GAGAAAAAGTATGGTAGTTT+TGG + chr4.3:14269627-14269646 None:intergenic 30.0%
!! TGGACTTTCTTTTTCATGTA+GGG + chr4.3:14269607-14269626 None:intergenic 30.0%
!! TTGGACTTTCTTTTTCATGT+AGG + chr4.3:14269608-14269627 None:intergenic 30.0%
AATTCATTGCTCATGTAGCT+TGG + chr4.3:14270586-14270605 None:intergenic 35.0%
ACCTCATCAACATTTCAAGA+TGG - chr4.3:14270321-14270340 MS.gene27799:intron 35.0%
AGGTGTGTCTAACAAGATTT+AGG + chr4.3:14269536-14269555 None:intergenic 35.0%
ATGTGAATGTGATAAGGATG+AGG + chr4.3:14270416-14270435 None:intergenic 35.0%
ATTCAAAGCCCATCAATTCA+AGG - chr4.3:14270440-14270459 MS.gene27799:intron 35.0%
CAACATTTCAAGATGGCAAA+TGG - chr4.3:14270328-14270347 MS.gene27799:intron 35.0%
GAGAAATGGAACCTAATTCA+TGG + chr4.3:14270276-14270295 None:intergenic 35.0%
GGTGTGTCTAACAAGATTTA+GGG + chr4.3:14269535-14269554 None:intergenic 35.0%
GTATTACAATACAAGGGCTT+AGG - chr4.3:14270163-14270182 MS.gene27799:intron 35.0%
TATTTGAAGAGTGAGAGTAG+CGG + chr4.3:14269777-14269796 None:intergenic 35.0%
TCAACAGCCATTAATACACA+AGG - chr4.3:14270616-14270635 MS.gene27799:intron 35.0%
TGTGAATGTGATAAGGATGA+GGG + chr4.3:14270415-14270434 None:intergenic 35.0%
TGTGGAAATTATAACTGTGC+TGG + chr4.3:14270381-14270400 None:intergenic 35.0%
TGTGTATTAATGGCTGTTGA+TGG + chr4.3:14270616-14270635 None:intergenic 35.0%
TTCTCTTCAAGAAATTGGCT+TGG + chr4.3:14270718-14270737 None:intergenic 35.0%
TTGTTCATGATATCTAAGGG+AGG + chr4.3:14270748-14270767 None:intergenic 35.0%
! ATCAGTTGAAGCACCAATTT+GGG + chr4.3:14268764-14268783 None:intergenic 35.0%
! CTTCCCAGCAATTTCATTTT+GGG - chr4.3:14269686-14269705 MS.gene27799:intron 35.0%
! CTTTTATGCATGCTGTTGAA+AGG - chr4.3:14268577-14268596 MS.gene27799:CDS 35.0%
! GAAATGTTGATGAGGTATCA+TGG + chr4.3:14270317-14270336 None:intergenic 35.0%
! TTCCCAGCAATTTCATTTTG+GGG - chr4.3:14269687-14269706 MS.gene27799:intron 35.0%
! TTTTACTTAGGCACCAAGAA+TGG - chr4.3:14270536-14270555 MS.gene27799:intron 35.0%
!!! TGGTGGTGATGGTATTTTTT+TGG - chr4.3:14269380-14269399 MS.gene27799:intron 35.0%
AACCTACAAACATTATCCCC+CGG + chr4.3:14269020-14269039 None:intergenic 40.0%
CACAGATCAACCCATGAATT+AGG - chr4.3:14270262-14270281 MS.gene27799:intron 40.0%
CATTTCAAGATGGCAAATGG+TGG - chr4.3:14270331-14270350 MS.gene27799:intron 40.0%
CCAAAATGAAATTGCTGGGA+AGG + chr4.3:14269688-14269707 None:intergenic 40.0%
CTTCAACTGATGAATGTGAG+AGG - chr4.3:14268773-14268792 MS.gene27799:CDS 40.0%
GCCATCTTGAAATGTTGATG+AGG + chr4.3:14270325-14270344 None:intergenic 40.0%
TGGAAACAACACAAGAAGCA+GGG - chr4.3:14270855-14270874 MS.gene27799:intron 40.0%
TTGAATTTGATCCCCCGTTT+CGG - chr4.3:14270929-14270948 MS.gene27799:CDS 40.0%
TTGGAAACAACACAAGAAGC+AGG - chr4.3:14270854-14270873 MS.gene27799:intron 40.0%
! ATGAGGTATCATGGACTGAT+GGG + chr4.3:14270308-14270327 None:intergenic 40.0%
! CATCAGTTGAAGCACCAATT+TGG + chr4.3:14268765-14268784 None:intergenic 40.0%
! CCTTCCCAGCAATTTCATTT+TGG - chr4.3:14269685-14269704 MS.gene27799:intron 40.0%
! TATCCTTAACTTGAAGCTGC+TGG + chr4.3:14270663-14270682 None:intergenic 40.0%
! TCCCAGCAATTTCATTTTGG+GGG - chr4.3:14269688-14269707 MS.gene27799:intron 40.0%
!! AAGCTGCTGGTTCAAAGTTA+TGG + chr4.3:14270650-14270669 None:intergenic 40.0%
!! AGAAATTGGCTTGGTCTCAT+AGG + chr4.3:14270709-14270728 None:intergenic 40.0%
ATGCATGCTGTTGAAAGGCT+TGG - chr4.3:14268582-14268601 MS.gene27799:CDS 45.0%
ATTCCCCGCAAAACAGGTTT+GGG - chr4.3:14269656-14269675 MS.gene27799:intron 45.0%
CCCCCCAAAATGAAATTGCT+GGG + chr4.3:14269692-14269711 None:intergenic 45.0%
CTCTTTATTCCCCGCAAAAC+AGG - chr4.3:14269650-14269669 MS.gene27799:intron 45.0%
TATTCCCCGCAAAACAGGTT+TGG - chr4.3:14269655-14269674 MS.gene27799:intron 45.0%
TCATGTCAAGAGTCACTTGC+AGG - chr4.3:14268808-14268827 MS.gene27799:CDS 45.0%
TCCCCCCAAAATGAAATTGC+TGG + chr4.3:14269693-14269712 None:intergenic 45.0%
! ATGGGCTACATGGTGAGAAA+TGG + chr4.3:14270290-14270309 None:intergenic 45.0%
! CCCAGCAATTTCATTTTGGG+GGG - chr4.3:14269689-14269708 MS.gene27799:intron 45.0%
! GAACCAGCAGCTTCAAGTTA+AGG - chr4.3:14270657-14270676 MS.gene27799:intron 45.0%
! GATGAGGTATCATGGACTGA+TGG + chr4.3:14270309-14270328 None:intergenic 45.0%
!! GCAATTTCATTTTGGGGGGA+AGG - chr4.3:14269693-14269712 MS.gene27799:intron 45.0%
!! TCATGGGTTGATCTGTGTGT+TGG + chr4.3:14270259-14270278 None:intergenic 45.0%
!! TTAATGTTGGTGGTGCATGC+AGG - chr4.3:14268731-14268750 MS.gene27799:CDS 45.0%
!! AAAATATATTAAAATAAAAG+GGG - chr4.3:14268934-14268953 MS.gene27799:intron 5.0%
!! GTAAAATATATTAAAATAAA+AGG - chr4.3:14268932-14268951 MS.gene27799:CDS 5.0%
!!! TATTTTTTTTATTGATTAAT+TGG + chr4.3:14269126-14269145 None:intergenic 5.0%
AACATCACTTAGACCCGCTG+AGG - chr4.3:14270825-14270844 MS.gene27799:intron 50.0%
AACTTGACGAGGCTGGACAA+AGG - chr4.3:14269500-14269519 MS.gene27799:intron 50.0%
AGCAAGAAACTTGACGAGGC+TGG - chr4.3:14269493-14269512 MS.gene27799:intron 50.0%
CACCGGGGGATAATGTTTGT+AGG - chr4.3:14269015-14269034 MS.gene27799:intron 50.0%
CATGCTGTTGAAAGGCTTGG+AGG - chr4.3:14268585-14268604 MS.gene27799:CDS 50.0%
CCTCGTCAAGTTTCTTGCTC+CGG + chr4.3:14269492-14269511 None:intergenic 50.0%
CTACAAACATTATCCCCCGG+TGG + chr4.3:14269017-14269036 None:intergenic 50.0%
GTGATAAGGATGAGGGATGC+AGG + chr4.3:14270408-14270427 None:intergenic 50.0%
GTTTCCAAAGCTCACCTCAG+CGG + chr4.3:14270842-14270861 None:intergenic 50.0%
TGCAGGAGCAACACCCAAAT+TGG - chr4.3:14268748-14268767 MS.gene27799:CDS 50.0%
TGGTCTCATAGGAGCAAGTG+TGG + chr4.3:14270698-14270717 None:intergenic 50.0%
TTCCCCGCAAAACAGGTTTG+GGG - chr4.3:14269657-14269676 MS.gene27799:intron 50.0%
TTTCCAAAGCTCACCTCAGC+GGG + chr4.3:14270841-14270860 None:intergenic 50.0%
!! TCATGGACTGATGGGCTACA+TGG + chr4.3:14270300-14270319 None:intergenic 50.0%
ACATTATCCCCCGGTGGACA+CGG + chr4.3:14269011-14269030 None:intergenic 55.0%
ATGAGGGATGCAGGAGACTG+TGG + chr4.3:14270399-14270418 None:intergenic 55.0%
CCGGAGCAAGAAACTTGACG+AGG - chr4.3:14269489-14269508 MS.gene27799:intron 55.0%
GACGAGGCTGGACAAAGGTA+AGG - chr4.3:14269505-14269524 MS.gene27799:intron 55.0%
TCAGGAGTCCACCTAAGCCT+AGG + chr4.3:14268546-14268565 None:intergenic 55.0%
TCCCCGCAAAACAGGTTTGG+GGG - chr4.3:14269658-14269677 MS.gene27799:intron 55.0%
! ACCCCCCAAACCTGTTTTGC+GGG + chr4.3:14269663-14269682 None:intergenic 55.0%
! CACCCCCCAAACCTGTTTTG+CGG + chr4.3:14269664-14269683 None:intergenic 55.0%
AGACCCGCTGAGGTGAGCTT+TGG - chr4.3:14270835-14270854 MS.gene27799:intron 60.0%
CCCCGCAAAACAGGTTTGGG+GGG - chr4.3:14269659-14269678 MS.gene27799:intron 60.0%
! CCCCCCAAACCTGTTTTGCG+GGG + chr4.3:14269662-14269681 None:intergenic 60.0%
AGGGTGATCCGTGTCCACCG+GGG - chr4.3:14269000-14269019 MS.gene27799:intron 65.0%
CAGGGTGATCCGTGTCCACC+GGG - chr4.3:14268999-14269018 MS.gene27799:intron 65.0%
GCAGGGTGATCCGTGTCCAC+CGG - chr4.3:14268998-14269017 MS.gene27799:intron 65.0%
GGGTGATCCGTGTCCACCGG+GGG - chr4.3:14269001-14269020 MS.gene27799:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 14268540 14270957 14268540 ID=MS.gene27799
chr4.3 mRNA 14268540 14270957 14268540 ID=MS.gene27799.t1;Parent=MS.gene27799
chr4.3 exon 14270882 14270957 14270882 ID=MS.gene27799.t1.exon1;Parent=MS.gene27799.t1
chr4.3 CDS 14270882 14270957 14270882 ID=cds.MS.gene27799.t1;Parent=MS.gene27799.t1
chr4.3 exon 14270668 14270744 14270668 ID=MS.gene27799.t1.exon2;Parent=MS.gene27799.t1
chr4.3 CDS 14270668 14270744 14270668 ID=cds.MS.gene27799.t1;Parent=MS.gene27799.t1
chr4.3 exon 14269976 14270013 14269976 ID=MS.gene27799.t1.exon3;Parent=MS.gene27799.t1
chr4.3 CDS 14269976 14270013 14269976 ID=cds.MS.gene27799.t1;Parent=MS.gene27799.t1
chr4.3 exon 14269036 14269242 14269036 ID=MS.gene27799.t1.exon4;Parent=MS.gene27799.t1
chr4.3 CDS 14269036 14269242 14269036 ID=cds.MS.gene27799.t1;Parent=MS.gene27799.t1
chr4.3 exon 14268540 14268951 14268540 ID=MS.gene27799.t1.exon5;Parent=MS.gene27799.t1
chr4.3 CDS 14268540 14268951 14268540 ID=cds.MS.gene27799.t1;Parent=MS.gene27799.t1
Gene Sequence

>MS.gene27799

ATGCCTAGGCTTAGGTGGACTCCTGATCTTCATCACTCTTTTATGCATGCTGTTGAAAGGCTTGGAGGCCAAGAGAGTAAGTATATTATATATTTTTTGTCTTAATTCTTTAGAAGAAAAAAACTATGCCCTAGATAATTGTGAAATTGTTTAATTTGGTTGAAGTTGAAAACTTTATGTTGTGTTCATTATTAATGTTGGTGGTGCATGCAGGAGCAACACCCAAATTGGTGCTTCAACTGATGAATGTGAGAGGACTCAGCATTGCTCATGTCAAGAGTCACTTGCAGGTATATAAGACAAAATTGAGGTAGATATATATGTTTCTTAAAAGAAAAAAAACAAAACACTGTACAACTTCAATCTTATAGCCACTGATATTTTTGTTTCACGTAAAATATATTAAAATAAAAGGGGGGAATAATTGAGAAACATATATTAGATCAATTAATTTTTCTGCAGGGTGATCCGTGTCCACCGGGGGATAATGTTTGTAGGTTTTTTTAATTTGATTTTGATGTTCTTTATGATAAGTTTTATAAAAAAATAAAAATCATTTATATATTCAAACAATTTGTACATTCCAATTAATCAATAAAAAAAATACCGAATGATTTATCTTGGGAATTGGATATTTTTTAAAATGATTTTTAGACACCAAAGAATAATATACCACTAGAGAGAAAAAAAAATAAAGATAGCGATGCAATAAATACAATAGATGGTTGGGGATGTGATATGAAGAAAGAGATAGAAAAATATGAAGTGTTGGATGAATGTAAAAAAAGTAAAAGTGTTTAAATATAATTGCTCTTTTTTTTTTTCCTTGATTTTTTTTTTTGGTGGTGATGGTATTTTTTTGGTAGTGATTTTATTTTTCCTGAAATTAAGTGTTGATATATGTATTTCATAAATCATTTTATGGCTATGCAATTTTCATTCAGATGTACCGGAGCAAGAAACTTGACGAGGCTGGACAAAGGTAAGGATAGCCCTAAATCTTGTTAGACACACCTTGAATTTTTTTTTGTCTGCCCTATAATGAAAAAAAAATTCTAAATCTGCCCTACATGAAAAAGAAAGTCCAAAACTACCATACTTTTTCTCTTTCTCTTTATTCCCCGCAAAACAGGTTTGGGGGGTGTCCTTCCCAGCAATTTCATTTTGGGGGGAAGGTCCTGTTTTTTTGTCTTATAAATCTACTACATGCTTTGAGAATATACAATCATTCAAACCGCTACTCTCACTCTTCAAATAAGTGACATCTTTTGGCTATGTGTACTATTCATATAATATATTTTGACCATATTTTTTTCACTGTTATATAAAAACAAATGGTGTCATATAAGATGTTGTGTGAATTTGTCTCTATCAACATTTTAAAAACATCAAATTTTATATTTTTTTTAATTACATAATTAAAAATATTAAAGATTAAAATTGTGCACATGTGTAAAACGGTCAATGATGTCATCTATTTTAAAATTGATGGAGTAAATGTTATGTTAGATTCCCAACTCTAATGATACTTACTTCTGTCTTTTAAAAAAAAACATTATTTTTTAGTAATTACATAAAAGAGTAGGTCTAATTAAAAATTTGTACTGGGTGTTCAAAATCAAAGTATTACAATACAAGGGCTTAGGGTTAGTTTACTTTAGCCGATTTTGTTTATTTTGACATTTATTCATATTTATTTACTTTGCAGTACTAAGTCCAACACACAGATCAACCCATGAATTAGGTTCCATTTCTCACCATGTAGCCCATCAGTCCATGATACCTCATCAACATTTCAAGATGGCAAATGGTGGAATTGTTCTAGAAACTAATTACAACCAGCACAGTTATAATTTCCACAGTCTCCTGCATCCCTCATCCTTATCACATTCACATTCACATTCAAAGCCCATCAATTCAAGGTAAACTATATATGCATACATTCTTATTTATTTATTTTATTTTTTTCCATTCAAGTGGCATTAAAGTTACATTTTTTTACTTAGGCACCAAGAATGGTACTTAAATCATCAATCTTGGAGACCAAGCTACATGAGCAATGAATTAGTTATGCCATCAACAGCCATTAATACACAAGGAAGATCATCCATAACTTTGAACCAGCAGCTTCAAGTTAAGGATACTACAGTCACTACCACACTTGCTCCTATGAGACCAAGCCAATTTCTTGAAGAGAAAAGATGGCCTCCCTTAGATATCATGAACAATCATCCATATTGGAAGAAAAATTTACATGATAATTTTTCTAATAATACTTGTGATAGAACATCACTTAGACCCGCTGAGGTGAGCTTTGGAAACAACACAAGAAGCAGGGAAAACATATCAAACATCAATAATGACCACAACTATTTTAATAGTTCAAAGATTGAATTTGATCCCCCGTTTCGGATTAAG
Protein sequence

>MS.gene27799.t1

MPRLRWTPDLHHSFMHAVERLGGQERATPKLVLQLMNVRGLSIAHVKSHLQMYRSKKLDEAGQSPTHRSTHELGSISHHVAHQSMIPHQHFKMANGGIVLETNYNQHSYNFHSLLHPSSLSHSHSHSKPINSRHQEWYLNHQSWRPSYMSNELVMPSTAINTQGRSSITLNQQLQVKDTTVTTTLAPMRPSQFLEEKRWPPLDIMNNHPYWKKNLHDNFSNNTCDRTSLRPAEVSFGNNTRSRENISNINNDHNYFNSSKIEFDPPFRIK