AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28064

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28064.t1 MTR_4g059870 33.721 172 100 5 109 280 51 208 1.94e-22 97.4
MS.gene28064.t1 MTR_8g017210 33.929 168 96 6 109 276 75 227 2.55e-22 97.4
MS.gene28064.t1 MTR_2g093960 31.633 196 119 6 109 304 45 225 9.82e-22 95.1
MS.gene28064.t1 MTR_1g093095 36.986 146 86 5 127 269 117 259 3.75e-21 91.7
MS.gene28064.t1 MTR_2g099990 32.370 173 102 6 109 281 59 216 1.86e-20 91.7
MS.gene28064.t1 MTR_2g090745 33.333 162 93 6 95 256 10 156 2.05e-19 88.6
MS.gene28064.t1 MTR_1g112270 32.450 151 91 5 106 256 54 193 2.41e-19 88.6
MS.gene28064.t1 MTR_1g112270 32.432 148 89 5 109 256 68 204 3.82e-19 88.2
MS.gene28064.t1 MTR_1g016010 35.811 148 85 5 109 256 54 191 7.91e-19 87.0
MS.gene28064.t1 MTR_2g099990 33.333 162 93 6 120 281 1 147 8.01e-19 86.7
MS.gene28064.t1 MTR_1g094115 33.775 151 89 5 106 256 57 196 1.11e-18 86.7
MS.gene28064.t1 MTR_1g094115 35.878 131 73 5 126 256 60 179 7.66e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28064.t1 AT1G34370 55.357 168 70 2 110 273 19 185 1.42e-64 205
MS.gene28064.t1 AT1G34370 56.805 169 66 3 110 273 229 395 1.32e-63 209
MS.gene28064.t1 AT1G34370 56.805 169 66 3 110 273 229 395 1.32e-63 209
MS.gene28064.t1 AT5G22890 39.557 316 134 9 17 276 57 371 3.47e-63 204
MS.gene28064.t1 AT3G45260 32.558 172 102 5 109 280 52 209 6.25e-23 98.6
MS.gene28064.t1 AT3G45260 32.558 172 102 5 109 280 52 209 6.25e-23 98.6
MS.gene28064.t1 AT1G08290 39.007 141 80 5 127 265 182 318 4.15e-22 95.1
MS.gene28064.t1 AT5G66730 36.486 148 83 5 109 256 45 181 4.28e-22 96.7
MS.gene28064.t1 AT5G60470 32.164 171 102 5 108 278 47 203 4.98e-22 96.3
MS.gene28064.t1 AT5G44160 35.135 148 85 5 109 256 50 186 5.93e-22 95.9
MS.gene28064.t1 AT5G60470 32.584 178 104 6 101 278 51 212 6.36e-22 95.9
MS.gene28064.t1 AT5G44160 35.135 148 85 5 109 256 11 147 6.83e-22 95.5
MS.gene28064.t1 AT5G60470 32.584 178 104 6 101 278 47 208 6.91e-22 95.9
MS.gene28064.t1 AT1G13290 37.415 147 87 4 127 271 103 246 7.44e-22 94.0
MS.gene28064.t1 AT5G03150 35.811 148 85 4 109 256 66 203 1.03e-21 95.5
MS.gene28064.t1 AT1G51220 39.189 148 83 6 127 272 180 322 1.43e-21 93.6
MS.gene28064.t1 AT3G50700 36.471 170 91 7 87 256 31 183 2.20e-21 94.4
MS.gene28064.t1 AT1G03840 35.099 151 87 5 106 256 49 188 2.32e-21 94.7
MS.gene28064.t1 AT1G55110 32.941 170 100 5 109 278 76 231 3.19e-21 94.0
MS.gene28064.t1 AT1G55110 32.941 170 100 5 109 278 76 231 3.19e-21 94.0
MS.gene28064.t1 AT1G55110 32.941 170 100 5 109 278 76 231 3.19e-21 94.0
MS.gene28064.t1 AT1G03840 35.135 148 85 5 109 256 54 190 3.54e-21 94.0
MS.gene28064.t1 AT3G20880 37.107 159 93 6 127 283 258 411 1.46e-20 91.7
MS.gene28064.t1 AT3G13810 35.135 148 86 4 109 256 83 220 7.43e-20 90.1
MS.gene28064.t1 AT3G13810 35.135 148 86 4 109 256 83 220 7.43e-20 90.1
MS.gene28064.t1 AT3G13810 35.135 148 86 4 109 256 78 215 7.47e-20 90.1
MS.gene28064.t1 AT3G13810 35.135 148 86 4 109 256 64 201 7.82e-20 90.1
MS.gene28064.t1 AT3G13810 35.135 148 86 4 109 256 84 221 1.15e-19 89.7
MS.gene28064.t1 AT3G13810 35.135 148 86 4 109 256 70 207 1.26e-19 89.4
MS.gene28064.t1 AT2G02080 30.526 190 111 8 101 286 61 233 3.40e-19 88.2
MS.gene28064.t1 AT2G02080 30.526 190 111 8 101 286 61 233 3.40e-19 88.2
MS.gene28064.t1 AT2G02080 30.526 190 111 8 101 286 61 233 3.40e-19 88.2
MS.gene28064.t1 AT2G02080 30.526 190 111 8 101 286 61 233 3.40e-19 88.2
MS.gene28064.t1 AT1G34790 37.748 151 86 7 118 265 137 282 3.61e-19 86.3
MS.gene28064.t1 AT4G02670 35.811 148 85 5 109 256 68 205 3.65e-19 87.4
MS.gene28064.t1 AT4G02670 35.811 148 85 5 109 256 66 203 3.90e-19 87.4
MS.gene28064.t1 AT2G02070 32.450 151 91 5 106 256 62 201 3.95e-19 88.2
MS.gene28064.t1 AT2G02070 32.450 151 91 5 106 256 62 201 3.95e-19 88.2
MS.gene28064.t1 AT1G14580 32.692 156 92 6 101 256 60 202 6.85e-19 87.0
MS.gene28064.t1 AT1G14580 32.692 156 92 6 101 256 60 202 6.85e-19 87.0
MS.gene28064.t1 AT1G14580 32.692 156 92 6 101 256 74 216 7.66e-19 87.0
MS.gene28064.t1 AT3G57670 35.862 145 86 6 127 269 219 358 3.43e-18 84.7
MS.gene28064.t1 AT1G25250 33.750 160 96 5 98 256 12 162 2.79e-17 81.6
MS.gene28064.t1 AT1G25250 35.570 149 86 5 109 256 46 185 3.02e-17 82.0
MS.gene28064.t1 AT2G01940 35.762 151 82 6 109 256 57 195 3.65e-17 82.0
MS.gene28064.t1 AT2G02080 30.409 171 100 7 120 286 1 156 4.03e-17 82.0
MS.gene28064.t1 AT2G02080 30.409 171 100 7 120 286 1 156 4.03e-17 82.0
MS.gene28064.t1 AT1G68130 35.570 149 85 5 109 256 54 192 8.14e-17 80.9
MS.gene28064.t1 AT2G01940 35.252 139 75 6 109 244 57 183 3.32e-14 73.2

Find 72 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGATTTGGATAAATTT+TGG 0.104736 4.4:+10823022 MS.gene28064:CDS
GGAGGAATTGTTACTGTTTC+TGG 0.121098 4.4:-10822334 None:intergenic
GGAAGGTTTCTTTGATGATT+TGG 0.263746 4.4:+10823011 MS.gene28064:CDS
CATGTCATCGTCAATGTAAT+TGG 0.286361 4.4:-10822389 None:intergenic
TCCACTTCTGCGAGATTTGC+GGG 0.292814 4.4:+10822482 MS.gene28064:CDS
TGCGTCGAGTTCCACAATTT+TGG 0.303992 4.4:-10822437 None:intergenic
ATGAAGGTTGCAAACGTAAC+AGG 0.321705 4.4:+10822638 MS.gene28064:CDS
GACAATGACTTCTCTTGAAA+TGG 0.326614 4.4:-10822708 None:intergenic
TCTAGGAAGGAGAAATTGTT+TGG 0.374626 4.4:+10822853 MS.gene28064:CDS
GACGAATTGTGGAGGAGATT+TGG 0.390719 4.4:-10822273 None:intergenic
AATCCATTTCGGTACTTAGT+TGG 0.394568 4.4:-10822174 None:intergenic
GAGGACTGTGTTTGTTTGAA+TGG 0.398093 4.4:-10822221 None:intergenic
ATCCATTTCGGTACTTAGTT+GGG 0.403084 4.4:-10822173 None:intergenic
TGTGTTTGTTTGAATGGATT+GGG 0.410517 4.4:-10822215 None:intergenic
CTGTGTTTGTTTGAATGGAT+TGG 0.411205 4.4:-10822216 None:intergenic
TGATTGCATGGATACGTTAC+CGG 0.422874 4.4:+10822990 MS.gene28064:CDS
TTTGGAATGGTAGTTATGAA+TGG 0.430613 4.4:-10822419 None:intergenic
GACGGCGCCGCCGGCAGAAG+AGG 0.442327 4.4:+10822936 MS.gene28064:CDS
TTACGTTTGCAACCTTCATA+AGG 0.442930 4.4:-10822634 None:intergenic
GCTTTGAATAGAATCCATTT+CGG 0.442981 4.4:-10822185 None:intergenic
CTCCCAACTAAGTACCGAAA+TGG 0.446469 4.4:+10822171 MS.gene28064:CDS
TTGGAATGGTAGTTATGAAT+GGG 0.447738 4.4:-10822418 None:intergenic
GAAGTTCAATCCGTTGAAAT+CGG 0.453409 4.4:+10822669 MS.gene28064:CDS
GGACACGTGGAGCGGTTTGA+AGG 0.462859 4.4:+10822874 MS.gene28064:CDS
TTCTGCGAGATTTGCGGGAA+AGG 0.464202 4.4:+10822487 MS.gene28064:CDS
TTCCACTTCTGCGAGATTTG+CGG 0.470603 4.4:+10822481 MS.gene28064:CDS
AATTGTGGAGGAGATTTGGT+TGG 0.477694 4.4:-10822269 None:intergenic
TAACTACCATTCCAAAATTG+TGG 0.494523 4.4:+10822426 MS.gene28064:CDS
GGAAAAGCAAGCGCGTTTAA+CGG 0.496000 4.4:+10822594 MS.gene28064:CDS
CCCACACCTTCCTCTTCTGC+CGG 0.506138 4.4:-10822946 None:intergenic
AAAATCAATGACGGCGCCGC+CGG 0.516248 4.4:+10822927 MS.gene28064:CDS
TTTCGGTACTTAGTTGGGAG+AGG 0.518556 4.4:-10822168 None:intergenic
GTCGAGTATAACTCGGTTGG+AGG 0.524381 4.4:-10822352 None:intergenic
AAATCATCAAAGAAACCTTC+CGG 0.527715 4.4:-10823009 None:intergenic
CCGGCGGTTGTTGAAGAGAA+AGG 0.528093 4.4:+10822904 MS.gene28064:CDS
GACGAAGGAAAATGATTGCA+TGG 0.537494 4.4:+10822978 MS.gene28064:CDS
TCCCGCAAATCTCGCAGAAG+TGG 0.537900 4.4:-10822483 None:intergenic
AAACAGAACACCGATTTCAA+CGG 0.538702 4.4:-10822679 None:intergenic
GAGTCACGTGAAACAGTGTA+GGG 0.546406 4.4:+10822792 MS.gene28064:CDS
GATGATGACTTGACGAATTG+TGG 0.554147 4.4:-10822284 None:intergenic
GGAGAAATTGTTTGGACACG+TGG 0.562238 4.4:+10822861 MS.gene28064:CDS
CAATTACATTGACGATGACA+TGG 0.570490 4.4:+10822390 MS.gene28064:CDS
CGAAAACTCACACGTGCGAT+CGG 0.572334 4.4:+10822731 MS.gene28064:CDS
TGTTACTGTTTCTGGACAAG+TGG 0.575238 4.4:-10822326 None:intergenic
CTCACACGTGCGATCGGTGT+CGG 0.577837 4.4:+10822737 MS.gene28064:CDS
CGTATGCACATGCGCGCGCA+CGG 0.585406 4.4:+10822532 MS.gene28064:CDS
CCTTTCTCTTCAACAACCGC+CGG 0.591632 4.4:-10822904 None:intergenic
TGCATGGATACGTTACCGGA+AGG 0.593308 4.4:+10822994 MS.gene28064:CDS
GCGCCGCCGGCAGAAGAGGA+AGG 0.598283 4.4:+10822940 MS.gene28064:CDS
AGAGTCACGTGAAACAGTGT+AGG 0.598901 4.4:+10822791 MS.gene28064:CDS
GCCGGCAGAAGAGGAAGGTG+TGG 0.600951 4.4:+10822945 MS.gene28064:CDS
GCGCGCGCATGTGCATACGC+AGG 0.610043 4.4:-10822528 None:intergenic
GCGGTTTGAAGGACACTCGC+CGG 0.611639 4.4:+10822885 MS.gene28064:CDS
CCTGTCGAGTATAACTCGGT+TGG 0.613205 4.4:-10822355 None:intergenic
CCAACCGAGTTATACTCGAC+AGG 0.616167 4.4:+10822355 MS.gene28064:CDS
CCGGCAGAAGAGGAAGGTGT+GGG 0.619763 4.4:+10822946 MS.gene28064:CDS
TCCACGTGGAAGTGTTCGTG+TGG 0.623338 4.4:+10822820 MS.gene28064:CDS
ACCACCTGTCGAGTATAACT+CGG 0.632991 4.4:-10822359 None:intergenic
ATGAAGCGTTACGTAGCGTG+AGG 0.636493 4.4:+10822572 MS.gene28064:CDS
AATTGTGGAACTCGACGCAG+TGG 0.638379 4.4:+10822441 MS.gene28064:CDS
TTTCTCCTTCCTAGAAAACG+TGG 0.641293 4.4:-10822845 None:intergenic
AATTGTTTGGACACGTGGAG+CGG 0.644804 4.4:+10822866 MS.gene28064:CDS
GTTTGAAGGACACTCGCCGG+CGG 0.649562 4.4:+10822888 MS.gene28064:CDS
ACACGTGCGATCGGTGTCGG+AGG 0.660805 4.4:+10822740 MS.gene28064:CDS
AGAGAAAGGAAAATCAATGA+CGG 0.662591 4.4:+10822918 MS.gene28064:CDS
TGAAGCGTTACGTAGCGTGA+GGG 0.667496 4.4:+10822573 MS.gene28064:CDS
ACACCTTCCTCTTCTGCCGG+CGG 0.685455 4.4:-10822943 None:intergenic
ACCGAGTTATACTCGACAGG+TGG 0.698259 4.4:+10822358 MS.gene28064:CDS
AGTGTAGGGGAGAGTCCACG+TGG 0.705085 4.4:+10822806 MS.gene28064:CDS
GATGACTTGACGAATTGTGG+AGG 0.731309 4.4:-10822281 None:intergenic
AGTCACGTGAAACAGTGTAG+GGG 0.737402 4.4:+10822793 MS.gene28064:CDS
TCCACACGAACACTTCCACG+TGG 0.747159 4.4:-10822821 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATGATTTGGATAAATTTT+GGG + chr4.4:10823023-10823042 MS.gene28064:CDS 20.0%
!!! TTGATGATTTGGATAAATTT+TGG + chr4.4:10823022-10823041 MS.gene28064:CDS 20.0%
AAATCATCAAAGAAACCTTC+CGG - chr4.4:10823012-10823031 None:intergenic 30.0%
AGAGAAAGGAAAATCAATGA+CGG + chr4.4:10822918-10822937 MS.gene28064:CDS 30.0%
TAACTACCATTCCAAAATTG+TGG + chr4.4:10822426-10822445 MS.gene28064:CDS 30.0%
TTGGAATGGTAGTTATGAAT+GGG - chr4.4:10822421-10822440 None:intergenic 30.0%
! GCTTTGAATAGAATCCATTT+CGG - chr4.4:10822188-10822207 None:intergenic 30.0%
! TGTGTTTGTTTGAATGGATT+GGG - chr4.4:10822218-10822237 None:intergenic 30.0%
! TTTGGAATGGTAGTTATGAA+TGG - chr4.4:10822422-10822441 None:intergenic 30.0%
!!! AGTGAGTCTAAGTTTTGTTT+GGG - chr4.4:10822247-10822266 None:intergenic 30.0%
AATCCATTTCGGTACTTAGT+TGG - chr4.4:10822177-10822196 None:intergenic 35.0%
ATCCATTTCGGTACTTAGTT+GGG - chr4.4:10822176-10822195 None:intergenic 35.0%
CAATTACATTGACGATGACA+TGG + chr4.4:10822390-10822409 MS.gene28064:CDS 35.0%
CATGTCATCGTCAATGTAAT+TGG - chr4.4:10822392-10822411 None:intergenic 35.0%
CTGTGTTTGTTTGAATGGAT+TGG - chr4.4:10822219-10822238 None:intergenic 35.0%
GAAGTTCAATCCGTTGAAAT+CGG + chr4.4:10822669-10822688 MS.gene28064:CDS 35.0%
GACAATGACTTCTCTTGAAA+TGG - chr4.4:10822711-10822730 None:intergenic 35.0%
TCTAGGAAGGAGAAATTGTT+TGG + chr4.4:10822853-10822872 MS.gene28064:CDS 35.0%
TTACGTTTGCAACCTTCATA+AGG - chr4.4:10822637-10822656 None:intergenic 35.0%
! CGATTTTCTTGTCCTTATGA+AGG + chr4.4:10822622-10822641 MS.gene28064:CDS 35.0%
! GGAAGGTTTCTTTGATGATT+TGG + chr4.4:10823011-10823030 MS.gene28064:CDS 35.0%
!! AAACAGAACACCGATTTCAA+CGG - chr4.4:10822682-10822701 None:intergenic 35.0%
!!! GAGTGAGTCTAAGTTTTGTT+TGG - chr4.4:10822248-10822267 None:intergenic 35.0%
!!! GTGAGTCTAAGTTTTGTTTG+GGG - chr4.4:10822246-10822265 None:intergenic 35.0%
ATGAAGGTTGCAAACGTAAC+AGG + chr4.4:10822638-10822657 MS.gene28064:CDS 40.0%
GACGAAGGAAAATGATTGCA+TGG + chr4.4:10822978-10822997 MS.gene28064:CDS 40.0%
GAGGACTGTGTTTGTTTGAA+TGG - chr4.4:10822224-10822243 None:intergenic 40.0%
GATGATGACTTGACGAATTG+TGG - chr4.4:10822287-10822306 None:intergenic 40.0%
GGAGGAATTGTTACTGTTTC+TGG - chr4.4:10822337-10822356 None:intergenic 40.0%
TGATTGCATGGATACGTTAC+CGG + chr4.4:10822990-10823009 MS.gene28064:CDS 40.0%
TTTCTCCTTCCTAGAAAACG+TGG - chr4.4:10822848-10822867 None:intergenic 40.0%
! AATTGTGGAGGAGATTTGGT+TGG - chr4.4:10822272-10822291 None:intergenic 40.0%
! TGTGGGTAGATTTTTGACGA+AGG + chr4.4:10822963-10822982 MS.gene28064:CDS 40.0%
!! CGAGTTCCACAATTTTGGAA+TGG - chr4.4:10822435-10822454 None:intergenic 40.0%
!! TGTTACTGTTTCTGGACAAG+TGG - chr4.4:10822329-10822348 None:intergenic 40.0%
!! TTAGTTGGGAGAGGTTTTGA+AGG - chr4.4:10822162-10822181 None:intergenic 40.0%
!!! AGTCTAAGTTTTGTTTGGGG+AGG - chr4.4:10822243-10822262 None:intergenic 40.0%
!!! TAGTTGGGAGAGGTTTTGAA+GGG - chr4.4:10822161-10822180 None:intergenic 40.0%
AATTGTTTGGACACGTGGAG+CGG + chr4.4:10822866-10822885 MS.gene28064:CDS 45.0%
ACCACCTGTCGAGTATAACT+CGG - chr4.4:10822362-10822381 None:intergenic 45.0%
AGAGTCACGTGAAACAGTGT+AGG + chr4.4:10822791-10822810 MS.gene28064:CDS 45.0%
AGTCACGTGAAACAGTGTAG+GGG + chr4.4:10822793-10822812 MS.gene28064:CDS 45.0%
GACGAATTGTGGAGGAGATT+TGG - chr4.4:10822276-10822295 None:intergenic 45.0%
GAGTCACGTGAAACAGTGTA+GGG + chr4.4:10822792-10822811 MS.gene28064:CDS 45.0%
GATGACTTGACGAATTGTGG+AGG - chr4.4:10822284-10822303 None:intergenic 45.0%
GGAAAAGCAAGCGCGTTTAA+CGG + chr4.4:10822594-10822613 MS.gene28064:CDS 45.0%
GGAGAAATTGTTTGGACACG+TGG + chr4.4:10822861-10822880 MS.gene28064:CDS 45.0%
TTCCACTTCTGCGAGATTTG+CGG + chr4.4:10822481-10822500 MS.gene28064:CDS 45.0%
TTTCGGTACTTAGTTGGGAG+AGG - chr4.4:10822171-10822190 None:intergenic 45.0%
! TGCGTCGAGTTCCACAATTT+TGG - chr4.4:10822440-10822459 None:intergenic 45.0%
!! CTCCCAACTAAGTACCGAAA+TGG + chr4.4:10822171-10822190 MS.gene28064:CDS 45.0%
!! TGGAACCACGTTTTCTAGGA+AGG + chr4.4:10822840-10822859 MS.gene28064:CDS 45.0%
!!! AGTTGGGAGAGGTTTTGAAG+GGG - chr4.4:10822160-10822179 None:intergenic 45.0%
AATTGTGGAACTCGACGCAG+TGG + chr4.4:10822441-10822460 MS.gene28064:CDS 50.0%
ACCGAGTTATACTCGACAGG+TGG + chr4.4:10822358-10822377 MS.gene28064:CDS 50.0%
CCAACCGAGTTATACTCGAC+AGG + chr4.4:10822355-10822374 MS.gene28064:CDS 50.0%
CCTGTCGAGTATAACTCGGT+TGG - chr4.4:10822358-10822377 None:intergenic 50.0%
CCTTTCTCTTCAACAACCGC+CGG - chr4.4:10822907-10822926 None:intergenic 50.0%
CGAAAACTCACACGTGCGAT+CGG + chr4.4:10822731-10822750 MS.gene28064:CDS 50.0%
GTCGAGTATAACTCGGTTGG+AGG - chr4.4:10822355-10822374 None:intergenic 50.0%
TCCACTTCTGCGAGATTTGC+GGG + chr4.4:10822482-10822501 MS.gene28064:CDS 50.0%
TGCATGGATACGTTACCGGA+AGG + chr4.4:10822994-10823013 MS.gene28064:CDS 50.0%
TTCTGCGAGATTTGCGGGAA+AGG + chr4.4:10822487-10822506 MS.gene28064:CDS 50.0%
! ATGAAGCGTTACGTAGCGTG+AGG + chr4.4:10822572-10822591 MS.gene28064:CDS 50.0%
! CGATCGCACGTGTGAGTTTT+CGG - chr4.4:10822733-10822752 None:intergenic 50.0%
! CGTGTGGAACCACGTTTTCT+AGG + chr4.4:10822836-10822855 MS.gene28064:CDS 50.0%
! GAAGAGTTTTGCGGTGGTGT+CGG + chr4.4:10822762-10822781 MS.gene28064:CDS 50.0%
! GTGTCGGAGGAAGAGTTTTG+CGG + chr4.4:10822753-10822772 MS.gene28064:CDS 50.0%
! TGAAGCGTTACGTAGCGTGA+GGG + chr4.4:10822573-10822592 MS.gene28064:CDS 50.0%
AAAATCAATGACGGCGCCGC+CGG + chr4.4:10822927-10822946 MS.gene28064:CDS 55.0%
CCGGCGGTTGTTGAAGAGAA+AGG + chr4.4:10822904-10822923 MS.gene28064:CDS 55.0%
TCCACACGAACACTTCCACG+TGG - chr4.4:10822824-10822843 None:intergenic 55.0%
TCCCGCAAATCTCGCAGAAG+TGG - chr4.4:10822486-10822505 None:intergenic 55.0%
! TCGGAGGAAGAGTTTTGCGG+TGG + chr4.4:10822756-10822775 MS.gene28064:CDS 55.0%
!! TCCACGTGGAAGTGTTCGTG+TGG + chr4.4:10822820-10822839 MS.gene28064:CDS 55.0%
ACACCTTCCTCTTCTGCCGG+CGG - chr4.4:10822946-10822965 None:intergenic 60.0%
AGTGTAGGGGAGAGTCCACG+TGG + chr4.4:10822806-10822825 MS.gene28064:CDS 60.0%
CCCACACCTTCCTCTTCTGC+CGG - chr4.4:10822949-10822968 None:intergenic 60.0%
CTCACACGTGCGATCGGTGT+CGG + chr4.4:10822737-10822756 MS.gene28064:CDS 60.0%
GCGGTTTGAAGGACACTCGC+CGG + chr4.4:10822885-10822904 MS.gene28064:CDS 60.0%
GGACACGTGGAGCGGTTTGA+AGG + chr4.4:10822874-10822893 MS.gene28064:CDS 60.0%
GTTTGAAGGACACTCGCCGG+CGG + chr4.4:10822888-10822907 MS.gene28064:CDS 60.0%
! CCGGCAGAAGAGGAAGGTGT+GGG + chr4.4:10822946-10822965 MS.gene28064:CDS 60.0%
CGTATGCACATGCGCGCGCA+CGG + chr4.4:10822532-10822551 MS.gene28064:CDS 65.0%
GCCGGCAGAAGAGGAAGGTG+TGG + chr4.4:10822945-10822964 MS.gene28064:CDS 65.0%
!! ACACGTGCGATCGGTGTCGG+AGG + chr4.4:10822740-10822759 MS.gene28064:CDS 65.0%
GCGCGCGCATGTGCATACGC+AGG - chr4.4:10822531-10822550 None:intergenic 70.0%
GCGCCGCCGGCAGAAGAGGA+AGG + chr4.4:10822940-10822959 MS.gene28064:CDS 75.0%
! GACGGCGCCGCCGGCAGAAG+AGG + chr4.4:10822936-10822955 MS.gene28064:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 10822112 10823050 10822112 ID=MS.gene28064
chr4.4 mRNA 10822112 10823050 10822112 ID=MS.gene28064.t1;Parent=MS.gene28064
chr4.4 exon 10822112 10823050 10822112 ID=MS.gene28064.t1.exon1;Parent=MS.gene28064.t1
chr4.4 CDS 10822112 10823050 10822112 ID=cds.MS.gene28064.t1;Parent=MS.gene28064.t1
Gene Sequence

>MS.gene28064

ATGCAAAATCTCACTGAAATTACTCATCAAACAAATGCAAATATTCCCCTTCAAAACCTCTCCCAACTAAGTACCGAAATGGATTCTATTCAAAGCTTCATATCCCAATCCATTCAAACAAACACAGTCCTCCCCAAACAAAACTTAGACTCACTCTCCAACCAAATCTCCTCCACAATTCGTCAAGTCATCATCAACGCAACTGCTCTTCTCGCCACTTGTCCAGAAACAGTAACAATTCCTCCAACCGAGTTATACTCGACAGGTGGTGAGAAAACCAATTACATTGACGATGACATGGCAAATCCCATTCATAACTACCATTCCAAAATTGTGGAACTCGACGCAGTGGAACTGCTTGCGAAGCACTTCCACTTCTGCGAGATTTGCGGGAAAGGTTTCACGCGCGATGCAAACCTGCGTATGCACATGCGCGCGCACGGAGAACAGTTCAAGACACATGAAGCGTTACGTAGCGTGAGGGAAAAGCAAGCGCGTTTAACGGCGACGCGATTTTCTTGTCCTTATGAAGGTTGCAAACGTAACAGGTTACACAAGAAGTTCAATCCGTTGAAATCGGTGTTCTGTTTGAAGAACCATTTCAAGAGAAGTCATTGTCCGAAAACTCACACGTGCGATCGGTGTCGGAGGAAGAGTTTTGCGGTGGTGTCGGATTTGAAGAGTCACGTGAAACAGTGTAGGGGAGAGTCCACGTGGAAGTGTTCGTGTGGAACCACGTTTTCTAGGAAGGAGAAATTGTTTGGACACGTGGAGCGGTTTGAAGGACACTCGCCGGCGGTTGTTGAAGAGAAAGGAAAATCAATGACGGCGCCGCCGGCAGAAGAGGAAGGTGTGGGTAGATTTTTGACGAAGGAAAATGATTGCATGGATACGTTACCGGAAGGTTTCTTTGATGATTTGGATAAATTTTGGGTTTGA
Protein sequence

>MS.gene28064.t1

MQNLTEITHQTNANIPLQNLSQLSTEMDSIQSFISQSIQTNTVLPKQNLDSLSNQISSTIRQVIINATALLATCPETVTIPPTELYSTGGEKTNYIDDDMANPIHNYHSKIVELDAVELLAKHFHFCEICGKGFTRDANLRMHMRAHGEQFKTHEALRSVREKQARLTATRFSCPYEGCKRNRLHKKFNPLKSVFCLKNHFKRSHCPKTHTCDRCRRKSFAVVSDLKSHVKQCRGESTWKCSCGTTFSRKEKLFGHVERFEGHSPAVVEEKGKSMTAPPAEEEGVGRFLTKENDCMDTLPEGFFDDLDKFWV