Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28191.t1 | GAU45400.1 | 52.6 | 268 | 69 | 4 | 1 | 267 | 1 | 211 | 2.30E-64 | 255.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28191.t1 | Q9SZL8 | 34.8 | 164 | 99 | 5 | 105 | 262 | 77 | 238 | 6.4e-15 | 82.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28191.t1 | A0A2Z6P7V3 | 52.6 | 268 | 69 | 4 | 1 | 267 | 1 | 211 | 1.6e-64 | 255.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene28191.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28191.t1 | MTR_3g080590 | 85.806 | 155 | 18 | 1 | 1 | 151 | 1 | 155 | 3.14e-83 | 246 |
| MS.gene28191.t1 | MTR_7g446520 | 44.118 | 170 | 80 | 1 | 94 | 263 | 81 | 235 | 4.90e-42 | 147 |
| MS.gene28191.t1 | MTR_7g028855 | 43.709 | 151 | 82 | 1 | 94 | 241 | 113 | 263 | 9.80e-34 | 127 |
| MS.gene28191.t1 | MTR_7g446480 | 53.933 | 89 | 41 | 0 | 175 | 263 | 140 | 228 | 1.61e-27 | 108 |
| MS.gene28191.t1 | MTR_7g446510 | 52.809 | 89 | 42 | 0 | 175 | 263 | 155 | 243 | 2.22e-24 | 100 |
| MS.gene28191.t1 | MTR_5g076070 | 29.518 | 166 | 114 | 2 | 98 | 260 | 50 | 215 | 1.77e-22 | 94.0 |
| MS.gene28191.t1 | MTR_1g070355 | 31.176 | 170 | 110 | 2 | 101 | 265 | 74 | 241 | 5.05e-22 | 95.5 |
| MS.gene28191.t1 | MTR_6g022080 | 26.471 | 170 | 122 | 1 | 94 | 260 | 78 | 247 | 2.01e-19 | 85.1 |
| MS.gene28191.t1 | MTR_8g035530 | 46.753 | 77 | 39 | 1 | 88 | 164 | 1 | 75 | 2.64e-17 | 74.7 |
| MS.gene28191.t1 | MTR_3g450850 | 29.651 | 172 | 109 | 4 | 107 | 266 | 53 | 224 | 7.01e-15 | 73.9 |
| MS.gene28191.t1 | MTR_0402s0040 | 32.121 | 165 | 103 | 6 | 104 | 263 | 29 | 189 | 1.34e-14 | 73.6 |
| MS.gene28191.t1 | MTR_4g035820 | 29.012 | 162 | 107 | 3 | 107 | 261 | 81 | 241 | 1.59e-12 | 67.4 |
| MS.gene28191.t1 | MTR_6g051425 | 29.697 | 165 | 101 | 6 | 108 | 262 | 24 | 183 | 3.27e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28191.t1 | AT5G28530 | 34.783 | 161 | 100 | 3 | 107 | 262 | 61 | 221 | 4.31e-19 | 87.0 |
| MS.gene28191.t1 | AT5G28530 | 34.783 | 161 | 100 | 3 | 107 | 262 | 61 | 221 | 4.34e-19 | 87.0 |
| MS.gene28191.t1 | AT5G28530 | 34.783 | 161 | 100 | 3 | 107 | 262 | 61 | 221 | 4.34e-19 | 87.0 |
| MS.gene28191.t1 | AT5G28530 | 34.783 | 161 | 100 | 3 | 107 | 262 | 61 | 221 | 5.85e-19 | 86.7 |
| MS.gene28191.t1 | AT5G28530 | 34.783 | 161 | 100 | 3 | 107 | 262 | 61 | 221 | 6.39e-19 | 86.7 |
| MS.gene28191.t1 | AT4G38180 | 31.696 | 224 | 141 | 7 | 46 | 262 | 20 | 238 | 1.66e-16 | 79.3 |
| MS.gene28191.t1 | AT1G10240 | 31.707 | 164 | 105 | 5 | 107 | 263 | 54 | 217 | 6.10e-14 | 72.0 |
| MS.gene28191.t1 | AT1G10240 | 31.707 | 164 | 105 | 5 | 107 | 263 | 54 | 217 | 6.10e-14 | 72.0 |
| MS.gene28191.t1 | AT3G07500 | 34.459 | 148 | 84 | 6 | 75 | 215 | 2 | 143 | 1.95e-11 | 62.4 |
Find 0 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGGACTTATATAATTTCA+TGG | + | 57333:12921-12940 | MS.gene28191:CDS | 20.0% |
| ! | AAATGATGATATAAGGGATA+TGG | + | 57333:12450-12469 | MS.gene28191:CDS | 25.0% |
| ! | ATATAATTTCATGGATCATC+AGG | + | 57333:12930-12949 | MS.gene28191:CDS | 25.0% |
| ! | ATGTGACAAATGATGATATA+AGG | + | 57333:12443-12462 | MS.gene28191:CDS | 25.0% |
| ! | GATCGAAAAAATAAGAAGAA+TGG | + | 57333:12619-12638 | MS.gene28191:CDS | 25.0% |
| ! | GTATCACTATATACATCAAA+AGG | - | 57333:12187-12206 | MS.gene28191:intergenic | 25.0% |
| ! | GTATTATTTGCAAAATACCA+CGG | + | 57333:12508-12527 | MS.gene28191:CDS | 25.0% |
| ! | TATTATTTGCAAAATACCAC+GGG | + | 57333:12509-12528 | MS.gene28191:CDS | 25.0% |
| ! | TGTGACAAATGATGATATAA+GGG | + | 57333:12444-12463 | MS.gene28191:CDS | 25.0% |
| ATGTGAAACGTGATTCTAAT+GGG | + | 57333:12551-12570 | MS.gene28191:CDS | 30.0% | |
| CAATTAGTATGCAATAAGGA+GGG | + | 57333:12589-12608 | MS.gene28191:CDS | 30.0% | |
| GTAAGAACATGTCATATCTT+GGG | + | 57333:12850-12869 | MS.gene28191:CDS | 30.0% | |
| TATCAACTTCACTCAATTTG+CGG | - | 57333:12798-12817 | MS.gene28191:intergenic | 30.0% | |
| TCAATTAGTATGCAATAAGG+AGG | + | 57333:12588-12607 | MS.gene28191:CDS | 30.0% | |
| TGTAAGAACATGTCATATCT+TGG | + | 57333:12849-12868 | MS.gene28191:CDS | 30.0% | |
| TGTATATAGTGATACAGATG+AGG | + | 57333:12192-12211 | MS.gene28191:CDS | 30.0% | |
| !!! | CATATCTTGGGTTTTTTGAT+GGG | + | 57333:12862-12881 | MS.gene28191:CDS | 30.0% |
| !!! | TCATATCTTGGGTTTTTTGA+TGG | + | 57333:12861-12880 | MS.gene28191:CDS | 30.0% |
| ACACCGATAGTGATACATAT+GGG | + | 57333:12242-12261 | MS.gene28191:CDS | 35.0% | |
| AGGACTTGGGTTTATCAAAA+AGG | + | 57333:12903-12922 | MS.gene28191:CDS | 35.0% | |
| ATATAGTGATACAGATGAGG+TGG | + | 57333:12195-12214 | MS.gene28191:CDS | 35.0% | |
| ATCAAATGGACAAAACGCAA+AGG | - | 57333:12766-12785 | MS.gene28191:intergenic | 35.0% | |
| GATGTGAAACGTGATTCTAA+TGG | + | 57333:12550-12569 | MS.gene28191:CDS | 35.0% | |
| GCGTCAATTAGTATGCAATA+AGG | + | 57333:12585-12604 | MS.gene28191:CDS | 35.0% | |
| GTGGAATTTAACCATGGTAA+TGG | + | 57333:12367-12386 | MS.gene28191:CDS | 35.0% | |
| TCACTCAATTTGCGGTAATT+AGG | - | 57333:12790-12809 | MS.gene28191:intergenic | 35.0% | |
| ! | GTTGATAAACAAGTTGCTAG+TGG | + | 57333:12811-12830 | MS.gene28191:CDS | 35.0% |
| !!! | GGTTTTTTGATGGGTCAAAA+GGG | + | 57333:12871-12890 | MS.gene28191:CDS | 35.0% |
| AACAACGATGTTCGTACTGA+TGG | + | 57333:12331-12350 | MS.gene28191:CDS | 40.0% | |
| AACGCAAAGGAGCTAATTCA+TGG | - | 57333:12753-12772 | MS.gene28191:intergenic | 40.0% | |
| AATGGTGTTTGCAATGAGCA+AGG | + | 57333:12385-12404 | MS.gene28191:CDS | 40.0% | |
| ACGACTTTGTTCGTACGATT+TGG | + | 57333:12698-12717 | MS.gene28191:CDS | 40.0% | |
| AGCTAATTCATGGTTGTGAG+AGG | - | 57333:12743-12762 | MS.gene28191:intergenic | 40.0% | |
| ATGGTGTTTGCAATGAGCAA+GGG | + | 57333:12386-12405 | MS.gene28191:CDS | 40.0% | |
| ATTTCATGGATCATCAGGCA+AGG | + | 57333:12935-12954 | MS.gene28191:CDS | 40.0% | |
| CAAGGGAAAGAGAACTTTGA+TGG | + | 57333:12403-12422 | MS.gene28191:CDS | 40.0% | |
| CACCCCATATGTATCACTAT+CGG | - | 57333:12248-12267 | MS.gene28191:intergenic | 40.0% | |
| CACCGATAGTGATACATATG+GGG | + | 57333:12243-12262 | MS.gene28191:CDS | 40.0% | |
| CATTGCAAACACCATTACCA+TGG | - | 57333:12381-12400 | MS.gene28191:intergenic | 40.0% | |
| CGCAGAACAATTAGTTCGAA+TGG | - | 57333:12662-12681 | MS.gene28191:intergenic | 40.0% | |
| GACACCGATAGTGATACATA+TGG | + | 57333:12241-12260 | MS.gene28191:CDS | 40.0% | |
| GCTAATTCATGGTTGTGAGA+GGG | - | 57333:12742-12761 | MS.gene28191:intergenic | 40.0% | |
| GGATATGGAATTCAACACAG+AGG | + | 57333:12465-12484 | MS.gene28191:CDS | 40.0% | |
| TGATAAACCCAAGTCCTTCA+TGG | - | 57333:12900-12919 | MS.gene28191:intergenic | 40.0% | |
| TGCGGTAATTAGGCATCAAA+TGG | - | 57333:12780-12799 | MS.gene28191:intergenic | 40.0% | |
| TTCGAATGGTTAGCGTAGAT+CGG | - | 57333:12648-12667 | MS.gene28191:intergenic | 40.0% | |
| !! | TTTTTGATGGGTCAAAAGGG+TGG | + | 57333:12874-12893 | MS.gene28191:CDS | 40.0% |
| !!! | GGGTTTTTTGATGGGTCAAA+AGG | + | 57333:12870-12889 | MS.gene28191:CDS | 40.0% |
| CCTTTCTTACTTCAAACCCG+TGG | - | 57333:12528-12547 | MS.gene28191:intergenic | 45.0% | |
| CGATAGTGATACATATGGGG+TGG | + | 57333:12246-12265 | MS.gene28191:CDS | 45.0% | |
| GACGGTGTGGAATTTAACCA+TGG | + | 57333:12361-12380 | MS.gene28191:CDS | 45.0% | |
| ! | CCACGGGTTTGAAGTAAGAA+AGG | + | 57333:12525-12544 | MS.gene28191:CDS | 45.0% |
| GCTAGTGGTCTACATTCGCA+AGG | + | 57333:12826-12845 | MS.gene28191:CDS | 50.0% | |
| ! | CGTACTGATGGAGATGCAGA+CGG | + | 57333:12343-12362 | MS.gene28191:CDS | 50.0% |
| AAGGGTGGCCATGAAGGACT+TGG | + | 57333:12889-12908 | MS.gene28191:CDS | 55.0% | |
| AGGGTGGCCATGAAGGACTT+GGG | + | 57333:12890-12909 | MS.gene28191:CDS | 55.0% | |
| GGTCAAAAGGGTGGCCATGA+AGG | + | 57333:12883-12902 | MS.gene28191:CDS | 55.0% | |
| TGATGGAGATGCAGACGGTG+TGG | + | 57333:12348-12367 | MS.gene28191:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57333 | gene | 12157 | 12957 | 12157 | ID=MS.gene28191 |
| 57333 | mRNA | 12157 | 12957 | 12157 | ID=MS.gene28191.t1;Parent=MS.gene28191 |
| 57333 | exon | 12157 | 12957 | 12157 | ID=MS.gene28191.t1.exon1;Parent=MS.gene28191.t1 |
| 57333 | CDS | 12157 | 12957 | 12157 | ID=cds.MS.gene28191.t1;Parent=MS.gene28191.t1 |
| Gene Sequence |
| Protein sequence |
