AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 97 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGAACGTGGCTAATTTC+TGG 0.218063 4.4:+9403388 MS.gene28370:CDS
AGAGCATAGAGTTTGGTTTC+AGG 0.263168 4.4:+9402798 MS.gene28370:CDS
GGCGGTGGTTGCTTCTATTC+AGG 0.264711 4.4:+9402865 MS.gene28370:CDS
TGTTCTTCCACCACTGGTAC+TGG 0.276664 4.4:-9403333 None:intergenic
AATTAGCCACGTTCTGAATC+TGG 0.296437 4.4:-9403383 None:intergenic
GAGTTGTGCGCATCATTAAT+TGG 0.323647 4.4:-9403267 None:intergenic
AAATTTGCTCGGCGAGCATT+TGG 0.329497 4.4:+9403701 MS.gene28370:CDS
GTCAAATAGAGCATAGAGTT+TGG 0.371033 4.4:+9402791 MS.gene28370:CDS
TTAAAACATGTGTCTCCAAC+TGG 0.380379 4.4:-9403512 None:intergenic
TTAGAGCACCATACCTGGTT+TGG 0.383982 4.4:-9401877 None:intergenic
CGATGTTGAATACCCTCCTT+TGG 0.387542 4.4:-9401846 None:intergenic
GATCATTATGAGCAACCATT+AGG 0.389483 4.4:+9402904 MS.gene28370:CDS
TAGAGCACCATACCTGGTTT+GGG 0.392335 4.4:-9401876 None:intergenic
CATTCCTGATGATGGTGATA+TGG 0.395267 4.4:+9403296 MS.gene28370:CDS
GCACGTGAGGCAGGTCGTTA+TGG 0.400336 4.4:+9403922 MS.gene28370:CDS
TCAGAACGTGGCTAATTTCT+GGG 0.416922 4.4:+9403389 MS.gene28370:CDS
GCTACTGCAGCGGCAGTTTG+TGG 0.430209 4.4:-9403360 None:intergenic
AATCGTAGGTTTAGACCTAA+TGG 0.433227 4.4:-9402919 None:intergenic
AAACATCCCGTTATCGTCAT+AGG 0.435778 4.4:-9402944 None:intergenic
AATTTGCTCGGCGAGCATTT+GGG 0.441935 4.4:+9403702 MS.gene28370:CDS
CATCATTAATTGGATTTGAC+GGG 0.443530 4.4:-9403257 None:intergenic
GCCTCACGTGCAGGACAGTT+GGG 0.444919 4.4:-9403910 None:intergenic
TCATTCCACTTAATATCGAT+TGG 0.456651 4.4:-9403776 None:intergenic
CGTTAGAGAGAACAGATTTG+TGG 0.458615 4.4:+9403850 MS.gene28370:CDS
TCCACCACTGGTACTGGGTC+AGG 0.460719 4.4:-9403327 None:intergenic
GTTCCACCGGGTTTCGAAGT+AGG 0.463343 4.4:+9403231 MS.gene28370:CDS
GATACCAGAGGGTCTAGGTT+TGG 0.463429 4.4:+9402820 MS.gene28370:CDS
GTTCTTCCACCACTGGTACT+GGG 0.464509 4.4:-9403332 None:intergenic
GATTGACCTATGACGATAAC+GGG 0.465542 4.4:+9402938 MS.gene28370:CDS
GCATCATTAATTGGATTTGA+CGG 0.475292 4.4:-9403258 None:intergenic
TGCCTCACGTGCAGGACAGT+TGG 0.476556 4.4:-9403911 None:intergenic
TTTAACGCTTTCTGCGTACA+TGG 0.478595 4.4:+9403530 MS.gene28370:CDS
AGTATCCAATCGATATTAAG+TGG 0.481943 4.4:+9403771 MS.gene28370:CDS
TCATCCATGTCAAGAGTGAA+TGG 0.486104 4.4:-9402976 None:intergenic
CTCATCTCGGATCGTGATGA+AGG 0.492063 4.4:-9403738 None:intergenic
GGCTGCTATGGATGTCGAAA+TGG 0.496679 4.4:+9403455 MS.gene28370:CDS
TCTCCTCCCATTCCTGATGA+TGG 0.497542 4.4:+9403288 MS.gene28370:CDS
TCTAGGTTTGGAGTATGTTA+CGG 0.497585 4.4:+9402832 MS.gene28370:CDS
CGATTGACCTATGACGATAA+CGG 0.500068 4.4:+9402937 MS.gene28370:CDS
AACTGTCCTGCACGTGAGGC+AGG 0.503776 4.4:+9403913 MS.gene28370:CDS
TTTATTTCCAGCAGTTCCAC+CGG 0.508663 4.4:+9403218 MS.gene28370:intron
TTCAGGATACCAGAGGGTCT+AGG 0.513988 4.4:+9402815 MS.gene28370:CDS
TACTCCAAACCTAGACCCTC+TGG 0.515790 4.4:-9402824 None:intergenic
ATTAGCCACGTTCTGAATCT+GGG 0.515846 4.4:-9403382 None:intergenic
CATATCACCATCATCAGGAA+TGG 0.516230 4.4:-9403295 None:intergenic
TGATAATGAGGAGGCTGCTA+TGG 0.520352 4.4:+9403443 MS.gene28370:CDS
GAAATGGATGTGGTAGATGC+TGG 0.524264 4.4:+9403471 MS.gene28370:CDS
AGCTTACGGCTCGTGTCCCA+TGG 0.534486 4.4:+9402746 MS.gene28370:intron
TAACGACCTGCCTCACGTGC+AGG 0.537930 4.4:-9403919 None:intergenic
TTTCGATATTGATGATAATG+AGG 0.538865 4.4:+9403431 MS.gene28370:CDS
ACTACCATATCACCATCATC+AGG 0.542080 4.4:-9403300 None:intergenic
TCTCGGATCGTGATGAAGGT+TGG 0.548455 4.4:-9403734 None:intergenic
TAAAACATGTGTCTCCAACT+GGG 0.552768 4.4:-9403511 None:intergenic
ATATCACCATCATCAGGAAT+GGG 0.554219 4.4:-9403294 None:intergenic
TTGTACCAGCAAAATTTGCT+CGG 0.557897 4.4:+9403690 MS.gene28370:CDS
AATACTGATGTCGCTTTATG+CGG 0.565255 4.4:-9403803 None:intergenic
GGCAGGTCGTTATGGTGTAA+AGG 0.566786 4.4:+9403930 MS.gene28370:CDS
ATTCACTCTTGACATGGATG+AGG 0.568212 4.4:+9402978 MS.gene28370:CDS
GCTTCCATTCACTCTTGACA+TGG 0.570881 4.4:+9402972 MS.gene28370:CDS
AAATGGATGTGGTAGATGCT+GGG 0.574403 4.4:+9403472 MS.gene28370:CDS
GTATGTTACGGAGAGATCAA+TGG 0.580224 4.4:+9402844 MS.gene28370:CDS
ACAGATTTGTGGAAGGCACA+AGG 0.581028 4.4:+9403861 MS.gene28370:CDS
TGACATGCAACCTTGAGCTA+TGG 0.582304 4.4:-9403029 None:intergenic
GTTGCTCATAATGATCAAGC+AGG 0.585077 4.4:-9402897 None:intergenic
TTATTTCCAGCAGTTCCACC+GGG 0.594229 4.4:+9403219 MS.gene28370:intron
TTATCATCAATATCGAAAGC+AGG 0.594729 4.4:-9403426 None:intergenic
ACCCAACTGTCCTGCACGTG+AGG 0.595873 4.4:+9403909 MS.gene28370:CDS
CCACCGGGTTTCGAAGTAGG+AGG 0.598715 4.4:+9403234 MS.gene28370:CDS
TGCACATGGCAATCCCCAGT+TGG 0.598828 4.4:+9403497 MS.gene28370:CDS
AAAACATGTGTCTCCAACTG+GGG 0.599659 4.4:-9403510 None:intergenic
AGGTAACTAATCGTCAATTG+AGG 0.606077 4.4:+9402998 MS.gene28370:CDS
CATCGCGGTCAACCCAAACC+AGG 0.607211 4.4:+9401864 MS.gene28370:CDS
TGTTCCTGACCCAGTACCAG+TGG 0.613436 4.4:+9403323 MS.gene28370:CDS
TACGGAGAGATCAATGGCGG+TGG 0.619550 4.4:+9402850 MS.gene28370:CDS
CAGATTTGTGGAAGGCACAA+GGG 0.620094 4.4:+9403862 MS.gene28370:CDS
CTGAATCTGGGCTACTGCAG+CGG 0.621166 4.4:-9403370 None:intergenic
TATCGTCATAGGTCAATCGT+AGG 0.623984 4.4:-9402933 None:intergenic
ACTCACGGTGGAACTCATCT+CGG 0.624872 4.4:-9403751 None:intergenic
CGATATTGATGATAATGAGG+AGG 0.629291 4.4:+9403434 MS.gene28370:CDS
TCCTGACCCAGTACCAGTGG+TGG 0.629390 4.4:+9403326 MS.gene28370:CDS
TAATATCGATTGGATACTCA+CGG 0.630661 4.4:-9403766 None:intergenic
AGAGAGAACAGATTTGTGGA+AGG 0.635968 4.4:+9403854 MS.gene28370:CDS
GTTTGGTTTCAGGATACCAG+AGG 0.643279 4.4:+9402808 MS.gene28370:CDS
TTTGGTTTCAGGATACCAGA+GGG 0.645318 4.4:+9402809 MS.gene28370:CDS
TCACCATCATCAGGAATGGG+AGG 0.646579 4.4:-9403291 None:intergenic
TAATTTCTGGGATGATGATG+AGG 0.648095 4.4:+9403401 MS.gene28370:CDS
GGAACTATGACCATAGCTCA+AGG 0.651950 4.4:+9403019 MS.gene28370:CDS
TATGGATGTCGAAATGGATG+TGG 0.668180 4.4:+9403461 MS.gene28370:CDS
GGGCCTCCTACTTCGAAACC+CGG 0.671468 4.4:-9403237 None:intergenic
CATGGTGAAATGCAGCAACA+TGG 0.687154 4.4:+9403548 MS.gene28370:CDS
GTAGATGCTGGGGATGCACA+TGG 0.689264 4.4:+9403483 MS.gene28370:CDS
AATGGATGTGGTAGATGCTG+GGG 0.695833 4.4:+9403473 MS.gene28370:CDS
AGTAGCCCAGATTCAGAACG+TGG 0.698194 4.4:+9403377 MS.gene28370:CDS
CCTCCTACTTCGAAACCCGG+TGG 0.702465 4.4:-9403234 None:intergenic
TGTTACGGAGAGATCAATGG+CGG 0.735510 4.4:+9402847 MS.gene28370:CDS
AAGGAGGGTATTCAACATCG+CGG 0.764822 4.4:+9401849 MS.gene28370:CDS
TATCGATTGGATACTCACGG+TGG 0.816467 4.4:-9403763 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAAGAAGAAAAAAAAA+CGG - chr4.4:9402067-9402086 None:intergenic 10.0%
!! AAAATTTGCAATACATTTAT+AGG + chr4.4:9402385-9402404 MS.gene28370:intron 15.0%
!! AAATTTGCAATACATTTATA+GGG + chr4.4:9402386-9402405 MS.gene28370:intron 15.0%
!! ATAAGAAATTGCAAAAAATT+TGG + chr4.4:9402500-9402519 MS.gene28370:intron 15.0%
!! TAAGAAATTGCAAAAAATTT+GGG + chr4.4:9402501-9402520 MS.gene28370:intron 15.0%
!!! TTATAGATTTTCAATACTTT+TGG + chr4.4:9402688-9402707 MS.gene28370:intron 15.0%
!! AACAAAAAAAACAATCATTC+TGG - chr4.4:9401946-9401965 None:intergenic 20.0%
!! AATTTGCAATACATTTATAG+GGG + chr4.4:9402387-9402406 MS.gene28370:intron 20.0%
!! CTTTATACAGTTATTTATTG+TGG + chr4.4:9403610-9403629 MS.gene28370:intron 20.0%
!! GGTTTATATAAGAAATTCAA+GGG + chr4.4:9402470-9402489 MS.gene28370:intron 20.0%
!! GTTTATATAAGAAATTCAAG+GGG + chr4.4:9402471-9402490 MS.gene28370:intron 20.0%
!! TAATTATAACCATTGTTCTA+AGG - chr4.4:9402560-9402579 None:intergenic 20.0%
!! TATATAAACCCCTATAAATT+GGG - chr4.4:9402460-9402479 None:intergenic 20.0%
!! TATTGAAAATCTATAAGCAA+AGG - chr4.4:9402684-9402703 None:intergenic 20.0%
!! TTATATAAACCCCTATAAAT+TGG - chr4.4:9402461-9402480 None:intergenic 20.0%
! AAAGAAATTGCCCAATTTAT+AGG + chr4.4:9402447-9402466 MS.gene28370:intron 25.0%
! AAGAAATTGCCCAATTTATA+GGG + chr4.4:9402448-9402467 MS.gene28370:intron 25.0%
! ACACAACAAAATACAAAGAA+TGG - chr4.4:9402322-9402341 None:intergenic 25.0%
! ATAAATAACTGTATAAAGCG+TGG - chr4.4:9403608-9403627 None:intergenic 25.0%
! GGGTTTATATAAGAAATTCA+AGG + chr4.4:9402469-9402488 MS.gene28370:intron 25.0%
! GTGTATGAGAAACAAAATTA+TGG - chr4.4:9402297-9402316 None:intergenic 25.0%
! TACAGTTATTTATTGTGGTT+TGG + chr4.4:9403615-9403634 MS.gene28370:intron 25.0%
! TTGTAGATTCCCTTAAAAAA+GGG + chr4.4:9402224-9402243 MS.gene28370:intron 25.0%
!! TTTCGATATTGATGATAATG+AGG + chr4.4:9403431-9403450 MS.gene28370:CDS 25.0%
!!! TATTTTTACGAACGTTTCAA+AGG + chr4.4:9402769-9402788 MS.gene28370:CDS 25.0%
AAACGTTCGTAAAAATACCA+TGG - chr4.4:9402766-9402785 None:intergenic 30.0%
AAATTGCAAAAAATTTGGGC+CGG + chr4.4:9402505-9402524 MS.gene28370:intron 30.0%
AACGTTCGTAAAAATACCAT+GGG - chr4.4:9402765-9402784 None:intergenic 30.0%
AGAAATTGCCCAATTTATAG+GGG + chr4.4:9402449-9402468 MS.gene28370:intron 30.0%
AGTATCCAATCGATATTAAG+TGG + chr4.4:9403771-9403790 MS.gene28370:CDS 30.0%
CATCATTAATTGGATTTGAC+GGG - chr4.4:9403260-9403279 None:intergenic 30.0%
CCTTCAAAAAAGAGAAAAAC+TGG - chr4.4:9402583-9402602 None:intergenic 30.0%
GCATCATTAATTGGATTTGA+CGG - chr4.4:9403261-9403280 None:intergenic 30.0%
GTTGTAGATTCCCTTAAAAA+AGG + chr4.4:9402223-9402242 MS.gene28370:intron 30.0%
TCATTCCACTTAATATCGAT+TGG - chr4.4:9403779-9403798 None:intergenic 30.0%
TGTTTGTATTCTGTAGCTTA+CGG + chr4.4:9402732-9402751 MS.gene28370:intron 30.0%
TTATCATCAATATCGAAAGC+AGG - chr4.4:9403429-9403448 None:intergenic 30.0%
! CAGCAGCATTAATTTGAAAT+TGG - chr4.4:9403140-9403159 None:intergenic 30.0%
! TAATATCGATTGGATACTCA+CGG - chr4.4:9403769-9403788 None:intergenic 30.0%
!!! ATTGCAAATTTTTTTTCGCC+CGG - chr4.4:9402377-9402396 None:intergenic 30.0%
!!! CCAGTTTTTCTCTTTTTTGA+AGG + chr4.4:9402580-9402599 MS.gene28370:intron 30.0%
!!! TTTTTTTTACCCTGCATATG+CGG - chr4.4:9402271-9402290 None:intergenic 30.0%
AAAATCTATAAGCAAAGGCG+AGG - chr4.4:9402679-9402698 None:intergenic 35.0%
AAATAAATCAGCAGCAGCAA+CGG - chr4.4:9403205-9403224 None:intergenic 35.0%
AAATCTATAAGCAAAGGCGA+GGG - chr4.4:9402678-9402697 None:intergenic 35.0%
AATCGTAGGTTTAGACCTAA+TGG - chr4.4:9402922-9402941 None:intergenic 35.0%
AATTAATGCTGCTGCTATTG+TGG + chr4.4:9403146-9403165 MS.gene28370:intron 35.0%
AGGTAACTAATCGTCAATTG+AGG + chr4.4:9402998-9403017 MS.gene28370:CDS 35.0%
ATATCACCATCATCAGGAAT+GGG - chr4.4:9403297-9403316 None:intergenic 35.0%
ATTAATGCTGCTGCTATTGT+GGG + chr4.4:9403147-9403166 MS.gene28370:intron 35.0%
GATCATTATGAGCAACCATT+AGG + chr4.4:9402904-9402923 MS.gene28370:CDS 35.0%
GGAATCTACAACTACAACAT+TGG - chr4.4:9402215-9402234 None:intergenic 35.0%
GTCAAATAGAGCATAGAGTT+TGG + chr4.4:9402791-9402810 MS.gene28370:CDS 35.0%
TAAAACATGTGTCTCCAACT+GGG - chr4.4:9403514-9403533 None:intergenic 35.0%
TTAAAACATGTGTCTCCAAC+TGG - chr4.4:9403515-9403534 None:intergenic 35.0%
! AAGCGACATCAGTATTTTTC+AGG + chr4.4:9403810-9403829 MS.gene28370:CDS 35.0%
! AGCATCGCATTTTGAATCAT+TGG - chr4.4:9401913-9401932 None:intergenic 35.0%
! AGCGACATCAGTATTTTTCA+GGG + chr4.4:9403811-9403830 MS.gene28370:CDS 35.0%
! CAAAGTATCTAAATGTACGC+AGG - chr4.4:9403062-9403081 None:intergenic 35.0%
! CGATATTGATGATAATGAGG+AGG + chr4.4:9403434-9403453 MS.gene28370:CDS 35.0%
! TCTAGGTTTGGAGTATGTTA+CGG + chr4.4:9402832-9402851 MS.gene28370:CDS 35.0%
! TTGTACCAGCAAAATTTGCT+CGG + chr4.4:9403690-9403709 MS.gene28370:CDS 35.0%
!! AATACTGATGTCGCTTTATG+CGG - chr4.4:9403806-9403825 None:intergenic 35.0%
!! GTATTTTGTTGTGTTGTGAG+TGG + chr4.4:9402328-9402347 MS.gene28370:intron 35.0%
!! TAATTTCTGGGATGATGATG+AGG + chr4.4:9403401-9403420 MS.gene28370:CDS 35.0%
!!! TTGCAAATTTTTTTTCGCCC+GGG - chr4.4:9402376-9402395 None:intergenic 35.0%
!!! TTTTTTTACCCTGCATATGC+GGG - chr4.4:9402270-9402289 None:intergenic 35.0%
AAAACATGTGTCTCCAACTG+GGG - chr4.4:9403513-9403532 None:intergenic 40.0%
AAACATCCCGTTATCGTCAT+AGG - chr4.4:9402947-9402966 None:intergenic 40.0%
AAATGGATGTGGTAGATGCT+GGG + chr4.4:9403472-9403491 MS.gene28370:CDS 40.0%
AATGCAACATCCACCAGATA+TGG - chr4.4:9401982-9402001 None:intergenic 40.0%
ACTACCATATCACCATCATC+AGG - chr4.4:9403303-9403322 None:intergenic 40.0%
AGAGAGAACAGATTTGTGGA+AGG + chr4.4:9403854-9403873 MS.gene28370:CDS 40.0%
ATTCACTCTTGACATGGATG+AGG + chr4.4:9402978-9402997 MS.gene28370:CDS 40.0%
CATATCACCATCATCAGGAA+TGG - chr4.4:9403298-9403317 None:intergenic 40.0%
CGATTGACCTATGACGATAA+CGG + chr4.4:9402937-9402956 MS.gene28370:CDS 40.0%
CGTTAGAGAGAACAGATTTG+TGG + chr4.4:9403850-9403869 MS.gene28370:CDS 40.0%
GAGTTGTGCGCATCATTAAT+TGG - chr4.4:9403270-9403289 None:intergenic 40.0%
GATTGACCTATGACGATAAC+GGG + chr4.4:9402938-9402957 MS.gene28370:CDS 40.0%
GTATGTTACGGAGAGATCAA+TGG + chr4.4:9402844-9402863 MS.gene28370:CDS 40.0%
GTTGCTCATAATGATCAAGC+AGG - chr4.4:9402900-9402919 None:intergenic 40.0%
TATCGTCATAGGTCAATCGT+AGG - chr4.4:9402936-9402955 None:intergenic 40.0%
TATGGATGTCGAAATGGATG+TGG + chr4.4:9403461-9403480 MS.gene28370:CDS 40.0%
TCAGAACGTGGCTAATTTCT+GGG + chr4.4:9403389-9403408 MS.gene28370:CDS 40.0%
TCATCCATGTCAAGAGTGAA+TGG - chr4.4:9402979-9402998 None:intergenic 40.0%
TTCAGAACGTGGCTAATTTC+TGG + chr4.4:9403388-9403407 MS.gene28370:CDS 40.0%
TTTAACGCTTTCTGCGTACA+TGG + chr4.4:9403530-9403549 MS.gene28370:CDS 40.0%
TTTATTTCCAGCAGTTCCAC+CGG + chr4.4:9403218-9403237 MS.gene28370:intron 40.0%
! AATTAGCCACGTTCTGAATC+TGG - chr4.4:9403386-9403405 None:intergenic 40.0%
! ACCAGATATGGAAGTGTATC+GGG - chr4.4:9401970-9401989 None:intergenic 40.0%
! AGAGCATAGAGTTTGGTTTC+AGG + chr4.4:9402798-9402817 MS.gene28370:CDS 40.0%
! ATTAGCCACGTTCTGAATCT+GGG - chr4.4:9403385-9403404 None:intergenic 40.0%
! CACCAGATATGGAAGTGTAT+CGG - chr4.4:9401971-9401990 None:intergenic 40.0%
! TTTGGTTTCAGGATACCAGA+GGG + chr4.4:9402809-9402828 MS.gene28370:CDS 40.0%
!! ATAACCATTGTTCTAAGGCC+CGG - chr4.4:9402555-9402574 None:intergenic 40.0%
!! CATTCCTGATGATGGTGATA+TGG + chr4.4:9403296-9403315 MS.gene28370:CDS 40.0%
!! GCGACATCAGTATTTTTCAG+GGG + chr4.4:9403812-9403831 MS.gene28370:CDS 40.0%
!! TGCATGCATTGATTCATCAG+TGG + chr4.4:9402001-9402020 MS.gene28370:intron 40.0%
!! TTTTGTTGTGTTGTGAGTGG+CGG + chr4.4:9402331-9402350 MS.gene28370:intron 40.0%
!!! ACATCAGTATTTTTCAGGGG+CGG + chr4.4:9403815-9403834 MS.gene28370:CDS 40.0%
!!! ACCAGGTTGCCCTTTTTTAA+GGG - chr4.4:9402236-9402255 None:intergenic 40.0%
AATGGATGTGGTAGATGCTG+GGG + chr4.4:9403473-9403492 MS.gene28370:CDS 45.0%
AGACGAAGACGAAGATGAGT+CGG - chr4.4:9403088-9403107 None:intergenic 45.0%
CATGGTGAAATGCAGCAACA+TGG + chr4.4:9403548-9403567 MS.gene28370:CDS 45.0%
CGATACACTTCCATATCTGG+TGG + chr4.4:9401969-9401988 MS.gene28370:intron 45.0%
CGATGTTGAATACCCTCCTT+TGG - chr4.4:9401849-9401868 None:intergenic 45.0%
GAAATGGATGTGGTAGATGC+TGG + chr4.4:9403471-9403490 MS.gene28370:CDS 45.0%
GCTTCCATTCACTCTTGACA+TGG + chr4.4:9402972-9402991 MS.gene28370:CDS 45.0%
GGAACTATGACCATAGCTCA+AGG + chr4.4:9403019-9403038 MS.gene28370:CDS 45.0%
TCCCGATACACTTCCATATC+TGG + chr4.4:9401966-9401985 MS.gene28370:intron 45.0%
TCCCTTAAAAAAGGGCAACC+TGG + chr4.4:9402232-9402251 MS.gene28370:intron 45.0%
TCGTCTTCGTCATCTGATGA+TGG + chr4.4:9403114-9403133 MS.gene28370:intron 45.0%
TGACATGCAACCTTGAGCTA+TGG - chr4.4:9403032-9403051 None:intergenic 45.0%
TGTTACGGAGAGATCAATGG+CGG + chr4.4:9402847-9402866 MS.gene28370:CDS 45.0%
TTATTTCCAGCAGTTCCACC+GGG + chr4.4:9403219-9403238 MS.gene28370:intron 45.0%
! AAATTTGCTCGGCGAGCATT+TGG + chr4.4:9403701-9403720 MS.gene28370:CDS 45.0%
! AATTTGCTCGGCGAGCATTT+GGG + chr4.4:9403702-9403721 MS.gene28370:CDS 45.0%
! ACAGATTTGTGGAAGGCACA+AGG + chr4.4:9403861-9403880 MS.gene28370:CDS 45.0%
! CAGATTTGTGGAAGGCACAA+GGG + chr4.4:9403862-9403881 MS.gene28370:CDS 45.0%
! CCAGGATTTTTATGAAGCCC+GGG + chr4.4:9402356-9402375 MS.gene28370:intron 45.0%
! GAAGATTAGAGCACCATACC+TGG - chr4.4:9401885-9401904 None:intergenic 45.0%
! GCCAGGATTTTTATGAAGCC+CGG + chr4.4:9402355-9402374 MS.gene28370:intron 45.0%
! GGATTTTGTTCTTCCACCAC+TGG - chr4.4:9403342-9403361 None:intergenic 45.0%
! GTTTGGTTTCAGGATACCAG+AGG + chr4.4:9402808-9402827 MS.gene28370:CDS 45.0%
! TAGAGCACCATACCTGGTTT+GGG - chr4.4:9401879-9401898 None:intergenic 45.0%
! TATCGATTGGATACTCACGG+TGG - chr4.4:9403766-9403785 None:intergenic 45.0%
! TGATAATGAGGAGGCTGCTA+TGG + chr4.4:9403443-9403462 MS.gene28370:CDS 45.0%
! TTAGAGCACCATACCTGGTT+TGG - chr4.4:9401880-9401899 None:intergenic 45.0%
!! AAGGAGGGTATTCAACATCG+CGG + chr4.4:9401849-9401868 MS.gene28370:CDS 45.0%
!! GATGCTGTTGGTGGTGATAA+TGG + chr4.4:9403177-9403196 MS.gene28370:intron 45.0%
!!! AGTATTTTTCAGGGGCGGTT+GGG + chr4.4:9403820-9403839 MS.gene28370:CDS 45.0%
!!! CACCAGGTTGCCCTTTTTTA+AGG - chr4.4:9402237-9402256 None:intergenic 45.0%
AAAAAATTTGGGCCGGAGCC+CGG + chr4.4:9402512-9402531 MS.gene28370:intron 50.0%
ACTCACGGTGGAACTCATCT+CGG - chr4.4:9403754-9403773 None:intergenic 50.0%
AGTAGCCCAGATTCAGAACG+TGG + chr4.4:9403377-9403396 MS.gene28370:CDS 50.0%
CCCGGGCTTCATAAAAATCC+TGG - chr4.4:9402359-9402378 None:intergenic 50.0%
CTCATCTCGGATCGTGATGA+AGG - chr4.4:9403741-9403760 None:intergenic 50.0%
GATACCAGAGGGTCTAGGTT+TGG + chr4.4:9402820-9402839 MS.gene28370:CDS 50.0%
GGCTGCTATGGATGTCGAAA+TGG + chr4.4:9403455-9403474 MS.gene28370:CDS 50.0%
GTTCTTCCACCACTGGTACT+GGG - chr4.4:9403335-9403354 None:intergenic 50.0%
TACTCCAAACCTAGACCCTC+TGG - chr4.4:9402827-9402846 None:intergenic 50.0%
TCACCATCATCAGGAATGGG+AGG - chr4.4:9403294-9403313 None:intergenic 50.0%
TCTCCTCCCATTCCTGATGA+TGG + chr4.4:9403288-9403307 MS.gene28370:CDS 50.0%
TCTCGGATCGTGATGAAGGT+TGG - chr4.4:9403737-9403756 None:intergenic 50.0%
TGTTCTTCCACCACTGGTAC+TGG - chr4.4:9403336-9403355 None:intergenic 50.0%
TTCAGGATACCAGAGGGTCT+AGG + chr4.4:9402815-9402834 MS.gene28370:CDS 50.0%
!!! CAGTATTTTTCAGGGGCGGT+TGG + chr4.4:9403819-9403838 MS.gene28370:CDS 50.0%
!!! GTATTTTTCAGGGGCGGTTG+GGG + chr4.4:9403821-9403840 MS.gene28370:CDS 50.0%
AAAAATTTGGGCCGGAGCCC+GGG + chr4.4:9402513-9402532 MS.gene28370:intron 55.0%
ACTAGAGCTCCCGCATATGC+AGG + chr4.4:9402258-9402277 MS.gene28370:intron 55.0%
CGGTCAACCCAAACCAGGTA+TGG + chr4.4:9401869-9401888 MS.gene28370:intron 55.0%
CTAGAGCTCCCGCATATGCA+GGG + chr4.4:9402259-9402278 MS.gene28370:intron 55.0%
CTGAATCTGGGCTACTGCAG+CGG - chr4.4:9403373-9403392 None:intergenic 55.0%
GCTGCTGCTATTGTGGGTTC+TGG + chr4.4:9403153-9403172 MS.gene28370:intron 55.0%
GGCGGTGGTTGCTTCTATTC+AGG + chr4.4:9402865-9402884 MS.gene28370:CDS 55.0%
GTAGATGCTGGGGATGCACA+TGG + chr4.4:9403483-9403502 MS.gene28370:CDS 55.0%
GTTCCACCGGGTTTCGAAGT+AGG + chr4.4:9403231-9403250 MS.gene28370:CDS 55.0%
TACGGAGAGATCAATGGCGG+TGG + chr4.4:9402850-9402869 MS.gene28370:CDS 55.0%
TGCACATGGCAATCCCCAGT+TGG + chr4.4:9403497-9403516 MS.gene28370:CDS 55.0%
TGTTCCTGACCCAGTACCAG+TGG + chr4.4:9403323-9403342 MS.gene28370:CDS 55.0%
! GCTCGCCGAGCAAATTTTGC+TGG - chr4.4:9403698-9403717 None:intergenic 55.0%
! GGTTCTGGTGATGCTGTTGG+TGG + chr4.4:9403168-9403187 MS.gene28370:intron 55.0%
! GTGGGTTCTGGTGATGCTGT+TGG + chr4.4:9403165-9403184 MS.gene28370:intron 55.0%
AACTGTCCTGCACGTGAGGC+AGG + chr4.4:9403913-9403932 MS.gene28370:CDS 60.0%
ACCCAACTGTCCTGCACGTG+AGG + chr4.4:9403909-9403928 MS.gene28370:CDS 60.0%
AGCTTACGGCTCGTGTCCCA+TGG + chr4.4:9402746-9402765 MS.gene28370:intron 60.0%
CATCGCGGTCAACCCAAACC+AGG + chr4.4:9401864-9401883 MS.gene28370:CDS 60.0%
CCACCGGGTTTCGAAGTAGG+AGG + chr4.4:9403234-9403253 MS.gene28370:CDS 60.0%
CCTCCTACTTCGAAACCCGG+TGG - chr4.4:9403237-9403256 None:intergenic 60.0%
CTCGCCGGGCCTTAGAACAA+TGG + chr4.4:9402548-9402567 MS.gene28370:intron 60.0%
GCACGTGAGGCAGGTCGTTA+TGG + chr4.4:9403922-9403941 MS.gene28370:CDS 60.0%
GCCTCACGTGCAGGACAGTT+GGG - chr4.4:9403913-9403932 None:intergenic 60.0%
GCTACTGCAGCGGCAGTTTG+TGG - chr4.4:9403363-9403382 None:intergenic 60.0%
GGGCCTCCTACTTCGAAACC+CGG - chr4.4:9403240-9403259 None:intergenic 60.0%
TAACGACCTGCCTCACGTGC+AGG - chr4.4:9403922-9403941 None:intergenic 60.0%
TCCTGACCCAGTACCAGTGG+TGG + chr4.4:9403326-9403345 MS.gene28370:CDS 60.0%
TCGAAACCCGGTGGAACTGC+TGG - chr4.4:9403228-9403247 None:intergenic 60.0%
TGCCTCACGTGCAGGACAGT+TGG - chr4.4:9403914-9403933 None:intergenic 60.0%
! TCCACCACTGGTACTGGGTC+AGG - chr4.4:9403330-9403349 None:intergenic 60.0%
! GTGTTGTGAGTGGCGGAGCC+AGG + chr4.4:9402338-9402357 MS.gene28370:intron 65.0%
! TGCGGGAGCTCTAGTGCACC+AGG - chr4.4:9402253-9402272 None:intergenic 65.0%
!! GTTCTAAGGCCCGGCGAGCC+CGG - chr4.4:9402546-9402565 None:intergenic 70.0%
!! TTCTAAGGCCCGGCGAGCCC+GGG - chr4.4:9402545-9402564 None:intergenic 70.0%
! CCGGAGCCCGGGCGAGTGCC+CGG + chr4.4:9402524-9402543 MS.gene28370:intron 85.0%
! CCGGGCACTCGCCCGGGCTC+CGG - chr4.4:9402527-9402546 None:intergenic 85.0%
! CGGAGCCCGGGCGAGTGCCC+GGG + chr4.4:9402525-9402544 MS.gene28370:intron 85.0%
! GCGAGCCCGGGCACTCGCCC+GGG - chr4.4:9402533-9402552 None:intergenic 85.0%
! GGCGAGCCCGGGCACTCGCC+CGG - chr4.4:9402534-9402553 None:intergenic 85.0%
! GGCGAGTGCCCGGGCTCGCC+GGG + chr4.4:9402534-9402553 MS.gene28370:intron 85.0%
! GGGCGAGTGCCCGGGCTCGC+CGG + chr4.4:9402533-9402552 MS.gene28370:intron 85.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 9401838 9403949 9401838 ID=MS.gene28370
chr4.4 mRNA 9401838 9403949 9401838 ID=MS.gene28370.t1;Parent=MS.gene28370
chr4.4 exon 9401838 9401885 9401838 ID=MS.gene28370.t1.exon1;Parent=MS.gene28370.t1
chr4.4 CDS 9401838 9401885 9401838 ID=cds.MS.gene28370.t1;Parent=MS.gene28370.t1
chr4.4 exon 9402748 9403063 9402748 ID=MS.gene28370.t1.exon2;Parent=MS.gene28370.t1
chr4.4 CDS 9402748 9403063 9402748 ID=cds.MS.gene28370.t1;Parent=MS.gene28370.t1
chr4.4 exon 9403229 9403569 9403229 ID=MS.gene28370.t1.exon3;Parent=MS.gene28370.t1
chr4.4 CDS 9403229 9403569 9403229 ID=cds.MS.gene28370.t1;Parent=MS.gene28370.t1
chr4.4 exon 9403689 9403949 9403689 ID=MS.gene28370.t1.exon4;Parent=MS.gene28370.t1
chr4.4 CDS 9403689 9403949 9403689 ID=cds.MS.gene28370.t1;Parent=MS.gene28370.t1
Gene Sequence

>MS.gene28370

ATGGTTTTCCAAAGGAGGGTATTCAACATCGCGGTCAACCCAAACCAGGTATGGTGCTCTAATCTTCAACAACCAATGATTCAAAATGCGATGCTAAAAAGTTCTCCAGAATGATTGTTTTTTTTGTTTCCCGATACACTTCCATATCTGGTGGATGTTGCATTGCATGCATTGATTCATCAGTGGCACAGTTTATTGATGACTCTGATTATGTTATTTTTTATTTCCGTTTTTTTTTCTTCTTAAATACAGATTGTTCAATATTGTAGTTGTAGATTGCATGCATTGATTCATCAGTGGCTCAGTATATTGATGACTCTGATTATGTTATTTATTTTTTCTTTTTTTTATTTTGTTTCTTAAATACATATTGTCCAATGTTGTAGTTGTAGATTCCCTTAAAAAAGGGCAACCTGGTGCACTAGAGCTCCCGCATATGCAGGGTAAAAAAAAAAACCATAATTTTGTTTCTCATACACTTCCATTCTTTGTATTTTGTTGTGTTGTGAGTGGCGGAGCCAGGATTTTTATGAAGCCCGGGCGAAAAAAAATTTGCAATACATTTATAGGGGTTTACATAAGAAATTGCAAGCAAATAATAAAAAATTTAAAGAAATTGCCCAATTTATAGGGGTTTATATAAGAAATTCAAGGGGATTTACATAAGAAATTGCAAAAAATTTGGGCCGGAGCCCGGGCGAGTGCCCGGGCTCGCCGGGCCTTAGAACAATGGTTATAATTACCAGTTTTTCTCTTTTTTGAAGGATTATTACTAGTTTTGTATTGCTCTTCTTTTTTGTATAATTTCTTCGAAACACACAACATTAAATAATAATACCCTCGCCTTTGCTTATAGATTTTCAATACTTTTGGTTGTAAACATACTGTTGTTGTTGTTTGTATTCTGTAGCTTACGGCTCGTGTCCCATGGTATTTTTACGAACGTTTCAAAGGTCAAATAGAGCATAGAGTTTGGTTTCAGGATACCAGAGGGTCTAGGTTTGGAGTATGTTACGGAGAGATCAATGGCGGTGGTTGCTTCTATTCAGGTCTTCAGAACCTGCTTGATCATTATGAGCAACCATTAGGTCTAAACCTACGATTGACCTATGACGATAACGGGATGTTTCATATGCTTCCATTCACTCTTGACATGGATGAGGTAACTAATCGTCAATTGAGGAACTATGACCATAGCTCAAGGTTGCATGTCAATGAGTTCCTGCGTACATTTAGATACTTTGATTCCGACTCATCTTCGTCTTCGTCTTCGTCTTCGTCTTCGTCATCTGATGATGGCCAATTTCAAATTAATGCTGCTGCTATTGTGGGTTCTGGTGATGCTGTTGGTGGTGATAATGGTGCCGTTGCTGCTGCTGATTTATTTCCAGCAGTTCCACCGGGTTTCGAAGTAGGAGGCCCGTCAAATCCAATTAATGATGCGCACAACTCTCCTCCCATTCCTGATGATGGTGATATGGTAGTTGTTCCTGACCCAGTACCAGTGGTGGAAGAACAAAATCCACAAACTGCCGCTGCAGTAGCCCAGATTCAGAACGTGGCTAATTTCTGGGATGATGATGAGGAACCTGCTTTCGATATTGATGATAATGAGGAGGCTGCTATGGATGTCGAAATGGATGTGGTAGATGCTGGGGATGCACATGGCAATCCCCAGTTGGAGACACATGTTTTAACGCTTTCTGCGTACATGGTGAAATGCAGCAACATGGTGAGTTACTTTGAAAAAATAAAAAAAAAACATATCCACGCTTTATACAGTTATTTATTGTGGTTTGGCAAGTGTGTGACTGTTGACATGAATTAATTATTGTTATCTGTCAACTGCAGCTTGTACCAGCAAAATTTGCTCGGCGAGCATTTGGGAATACAAGACCAACCTTCATCACGATCCGAGATGAGTTCCACCGTGAGTATCCAATCGATATTAAGTGGAATGAAAAACCGCATAAAGCGACATCAGTATTTTTCAGGGGCGGTTGGGGTGAATTCGTTAGAGAGAACAGATTTGTGGAAGGCACAAGGGTGTTGCTCACTTTAAACAAGACTAACCCAACTGTCCTGCACGTGAGGCAGGTCGTTATGGTGTAA
Protein sequence

>MS.gene28370.t1

MVFQRRVFNIAVNPNQLTARVPWYFYERFKGQIEHRVWFQDTRGSRFGVCYGEINGGGCFYSGLQNLLDHYEQPLGLNLRLTYDDNGMFHMLPFTLDMDEVTNRQLRNYDHSSRLHVNEFLPVPPGFEVGGPSNPINDAHNSPPIPDDGDMVVVPDPVPVVEEQNPQTAAAVAQIQNVANFWDDDEEPAFDIDDNEEAAMDVEMDVVDAGDAHGNPQLETHVLTLSAYMVKCSNMLVPAKFARRAFGNTRPTFITIRDEFHREYPIDIKWNEKPHKATSVFFRGGWGEFVRENRFVEGTRVLLTLNKTNPTVLHVRQVVMV