AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28382

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28382.t1 MTR_8g093790 91.892 185 8 3 1 178 1 185 1.01e-119 336
MS.gene28382.t1 MTR_3g096400 51.220 205 67 9 7 178 4 208 3.51e-57 178
MS.gene28382.t1 MTR_4g094302 46.032 189 74 6 10 178 11 191 4.34e-49 157
MS.gene28382.t1 MTR_4g094302 49.167 120 51 3 10 119 11 130 6.24e-36 123
MS.gene28382.t1 MTR_4g081870 38.542 192 91 7 2 169 1 189 2.15e-31 114
MS.gene28382.t1 MTR_2g080010 37.569 181 80 5 10 157 15 195 1.15e-30 114
MS.gene28382.t1 MTR_8g094580 43.038 158 67 6 5 139 2 159 1.76e-29 110
MS.gene28382.t1 MTR_5g041940 37.778 180 87 6 8 162 1 180 2.52e-29 108
MS.gene28382.t1 MTR_7g005280 40.854 164 72 7 8 150 13 172 5.19e-29 108
MS.gene28382.t1 MTR_2157s0010 34.946 186 88 6 4 157 2 186 2.08e-28 106
MS.gene28382.t1 MTR_3g088110 37.430 179 90 6 6 162 3 181 1.30e-27 105
MS.gene28382.t1 MTR_2g093810 36.842 171 82 5 3 147 7 177 4.75e-27 104
MS.gene28382.t1 MTR_7g100990 39.306 173 78 7 6 152 7 178 5.64e-27 104
MS.gene28382.t1 MTR_4g089135 37.853 177 65 6 10 141 15 191 4.63e-26 102
MS.gene28382.t1 MTR_3g096920 39.490 157 73 6 5 139 2 158 1.41e-25 99.8
MS.gene28382.t1 MTR_8g059170 40.244 164 68 7 6 143 10 169 3.44e-25 99.4
MS.gene28382.t1 MTR_3g435150 37.423 163 80 5 10 150 16 178 4.72e-25 98.6
MS.gene28382.t1 MTR_2g079990 38.608 158 77 5 6 143 12 169 5.72e-25 99.4
MS.gene28382.t1 MTR_7g085220 37.647 170 72 7 2 138 1 169 6.32e-25 99.0
MS.gene28382.t1 MTR_6g084430 38.667 150 74 5 10 141 5 154 1.89e-24 97.4
MS.gene28382.t1 MTR_4g108760 39.073 151 70 5 10 138 17 167 2.64e-24 97.4
MS.gene28382.t1 MTR_4g035590 34.031 191 101 8 4 169 2 192 2.63e-23 94.7
MS.gene28382.t1 MTR_3g109340 36.905 168 86 5 10 157 22 189 4.44e-23 94.4
MS.gene28382.t1 MTR_1g008740 40.397 151 68 5 10 138 27 177 6.04e-23 94.0
MS.gene28382.t1 MTR_1g008740 40.397 151 68 5 10 138 13 163 7.88e-23 93.6
MS.gene28382.t1 MTR_3g109340 36.095 169 87 5 10 157 22 190 8.61e-23 93.6
MS.gene28382.t1 MTR_3g098810 37.037 162 74 6 9 142 13 174 1.65e-22 90.9
MS.gene28382.t1 MTR_8g099750 35.443 158 77 5 9 141 13 170 1.73e-22 90.5
MS.gene28382.t1 MTR_2g062730 34.706 170 89 7 1 148 1 170 2.91e-22 91.7
MS.gene28382.t1 MTR_3g064580 38.312 154 72 6 8 138 4 157 1.33e-21 91.3
MS.gene28382.t1 MTR_3g070030 37.059 170 81 7 8 154 17 183 1.53e-21 90.1
MS.gene28382.t1 MTR_3g096920 39.394 132 65 4 5 121 2 133 1.75e-21 89.4
MS.gene28382.t1 MTR_7g085260 32.338 201 102 6 3 177 10 202 2.40e-21 89.7
MS.gene28382.t1 MTR_1g069805 35.897 156 82 5 8 145 8 163 2.49e-21 88.2
MS.gene28382.t1 MTR_2g064470 37.255 153 71 7 8 138 9 158 3.75e-21 88.2
MS.gene28382.t1 MTR_2g079990 32.796 186 77 5 6 143 12 197 7.41e-21 88.6
MS.gene28382.t1 MTR_6g477900 37.419 155 75 6 6 138 2 156 8.46e-21 86.3
MS.gene28382.t1 MTR_6g012670 33.728 169 90 5 4 150 14 182 8.53e-21 87.4
MS.gene28382.t1 MTR_7g097090 36.735 147 75 5 8 136 8 154 1.49e-20 86.3
MS.gene28382.t1 MTR_5g021710 33.333 192 96 9 10 173 8 195 4.30e-20 85.9
MS.gene28382.t1 MTR_4g036030 34.197 193 98 9 10 178 7 194 4.65e-20 85.9
MS.gene28382.t1 MTR_2g078700 37.908 153 71 6 10 138 23 175 5.85e-20 85.9
MS.gene28382.t1 MTR_8g076110 35.758 165 81 8 10 151 6 168 6.25e-20 85.1
MS.gene28382.t1 MTR_2g064090 32.418 182 98 8 6 162 2 183 8.61e-20 85.1
MS.gene28382.t1 MTR_5g076850 37.179 156 74 7 6 138 2 156 1.01e-19 85.9
MS.gene28382.t1 MTR_1g090720 34.395 157 75 6 10 138 42 198 1.15e-19 85.5
MS.gene28382.t1 MTR_3g098810 39.695 131 61 4 9 121 13 143 1.33e-19 81.6
MS.gene28382.t1 MTR_5g012080 34.839 155 78 6 10 141 7 161 1.36e-19 84.7
MS.gene28382.t1 MTR_3g070040 37.908 153 74 6 8 139 6 158 2.01e-19 84.0
MS.gene28382.t1 MTR_6g011860 34.146 164 74 7 10 141 15 176 9.14e-19 82.8
MS.gene28382.t1 MTR_7g011120 34.783 161 76 6 8 139 6 166 6.43e-18 79.7
MS.gene28382.t1 MTR_2g068880 30.723 166 79 6 12 141 24 189 6.83e-18 79.7
MS.gene28382.t1 MTR_1g096430 36.478 159 76 8 2 138 1 156 7.17e-18 79.7
MS.gene28382.t1 MTR_4g101680 33.987 153 80 6 10 141 7 159 8.06e-18 79.7
MS.gene28382.t1 MTR_4g134460 36.242 149 70 6 12 138 56 201 1.05e-17 79.7
MS.gene28382.t1 MTR_3g096140 37.333 150 72 8 12 139 9 158 1.22e-17 78.6
MS.gene28382.t1 MTR_3g116070 37.748 151 72 5 10 141 23 170 1.38e-17 79.0
MS.gene28382.t1 MTR_8g024480 31.902 163 79 7 9 139 14 176 1.52e-17 79.3
MS.gene28382.t1 MTR_5g040420 33.125 160 77 6 12 141 16 175 1.86e-17 79.0
MS.gene28382.t1 MTR_3g096140 37.333 150 72 8 12 139 9 158 2.40e-17 78.6
MS.gene28382.t1 MTR_2g068920 30.357 168 81 6 12 143 23 190 2.41e-17 77.8
MS.gene28382.t1 MTR_5g090970 29.268 164 84 5 12 143 20 183 3.28e-17 77.8
MS.gene28382.t1 MTR_8g467490 32.278 158 76 6 12 138 16 173 3.28e-17 78.2
MS.gene28382.t1 MTR_6g032770 34.591 159 80 5 4 138 12 170 4.11e-17 77.8
MS.gene28382.t1 MTR_2g086880 36.111 144 66 8 12 135 20 157 5.40e-17 78.2
MS.gene28382.t1 MTR_8g023840 34.932 146 74 6 10 135 11 155 7.34e-17 77.4
MS.gene28382.t1 MTR_8g023930 31.548 168 91 8 10 157 24 187 1.21e-16 77.0
MS.gene28382.t1 MTR_7g011130 34.132 167 75 6 8 139 17 183 1.28e-16 76.3
MS.gene28382.t1 MTR_2g086880 37.594 133 61 7 12 124 20 150 1.78e-16 76.6
MS.gene28382.t1 MTR_4g075980 34.545 165 76 8 12 149 17 176 2.46e-16 75.1
MS.gene28382.t1 MTR_5g014300 33.129 163 80 7 2 138 1 160 4.41e-16 74.7
MS.gene28382.t1 MTR_5g090970 29.268 164 83 6 12 143 20 182 4.59e-16 74.3
MS.gene28382.t1 MTR_4g098630 31.492 181 99 7 8 163 4 184 6.48e-16 74.3
MS.gene28382.t1 MTR_8g093580 32.680 153 81 7 9 139 5 157 3.89e-15 72.8
MS.gene28382.t1 MTR_5g069030 33.548 155 77 7 9 139 24 176 4.17e-15 72.8
MS.gene28382.t1 MTR_8g063550 32.692 156 77 8 9 139 5 157 1.21e-14 71.2
MS.gene28382.t1 MTR_8g063550 32.692 156 77 8 9 139 5 157 1.72e-14 70.9
MS.gene28382.t1 MTR_8g063550 32.692 156 77 8 9 139 5 157 2.52e-14 69.7
MS.gene28382.t1 MTR_8g023860 31.507 146 79 6 10 135 12 156 4.33e-14 69.3
MS.gene28382.t1 MTR_8g023900 29.560 159 88 7 13 151 196 350 1.67e-13 67.8
MS.gene28382.t1 MTR_8g023880 31.928 166 78 6 5 138 2 164 2.69e-13 66.2
MS.gene28382.t1 MTR_1g097300 34.127 126 65 5 12 119 21 146 6.68e-12 63.2
MS.gene28382.t1 MTR_4g052620 31.410 156 78 8 10 136 5 160 6.25e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28382.t1 AT5G64530 46.465 198 67 7 8 177 1 187 1.49e-50 161
MS.gene28382.t1 AT5G64530 51.261 119 50 3 8 119 1 118 1.69e-36 124
MS.gene28382.t1 AT1G61110 41.916 167 64 6 10 143 16 182 8.72e-30 111
MS.gene28382.t1 AT1G77450 42.236 161 71 6 1 139 1 161 3.06e-29 108
MS.gene28382.t1 AT2G33480 41.667 156 70 6 8 145 13 165 3.91e-29 108
MS.gene28382.t1 AT2G33480 41.772 158 69 7 8 145 13 167 5.31e-29 108
MS.gene28382.t1 AT1G69490 37.766 188 85 7 2 165 1 180 6.08e-28 105
MS.gene28382.t1 AT1G01720 43.226 155 65 6 8 139 5 159 1.41e-27 105
MS.gene28382.t1 AT5G39610 39.130 161 76 5 1 139 11 171 9.97e-27 102
MS.gene28382.t1 AT3G04070 37.912 182 79 8 1 150 1 180 2.00e-26 103
MS.gene28382.t1 AT5G13180 39.873 158 65 7 8 141 12 163 6.26e-26 100
MS.gene28382.t1 AT1G52890 37.278 169 82 6 6 154 10 174 1.00e-25 100
MS.gene28382.t1 AT3G18400 38.065 155 77 5 10 145 5 159 1.23e-25 100
MS.gene28382.t1 AT5G63790 40.252 159 71 6 5 139 45 203 1.24e-25 100
MS.gene28382.t1 AT5G63790 40.252 159 71 6 5 139 55 213 1.48e-25 100
MS.gene28382.t1 AT3G15500 35.673 171 90 5 6 156 10 180 2.28e-25 99.8
MS.gene28382.t1 AT2G24430 40.667 150 68 5 10 138 16 165 5.17e-25 99.0
MS.gene28382.t1 AT2G24430 40.667 150 68 5 10 138 16 165 5.17e-25 99.0
MS.gene28382.t1 AT5G61430 38.462 156 74 5 5 138 11 166 8.46e-25 98.6
MS.gene28382.t1 AT3G12977 33.721 172 91 6 8 158 16 185 1.00e-24 97.4
MS.gene28382.t1 AT5G07680 39.735 151 69 5 10 138 17 167 1.28e-24 98.2
MS.gene28382.t1 AT5G07680 39.735 151 69 5 10 138 3 153 1.31e-24 97.8
MS.gene28382.t1 AT4G27410 36.585 205 97 10 6 178 10 213 1.71e-24 97.1
MS.gene28382.t1 AT5G08790 40.252 159 71 6 5 139 2 160 4.56e-24 95.9
MS.gene28382.t1 AT3G04070 34.848 198 79 8 1 150 1 196 4.86e-24 97.1
MS.gene28382.t1 AT3G15510 32.821 195 92 6 14 175 21 209 1.55e-23 95.5
MS.gene28382.t1 AT5G18270 37.736 159 76 5 3 138 14 172 2.99e-23 94.4
MS.gene28382.t1 AT3G04060 33.838 198 101 8 3 177 13 203 3.14e-23 94.4
MS.gene28382.t1 AT5G18270 37.107 159 77 5 3 138 14 172 7.46e-23 93.2
MS.gene28382.t1 AT1G56010 35.484 155 76 6 8 138 17 171 1.13e-22 92.8
MS.gene28382.t1 AT4G10350 37.888 161 77 7 2 139 1 161 2.17e-22 92.0
MS.gene28382.t1 AT1G79580 37.059 170 80 8 2 144 3 172 3.80e-22 92.0
MS.gene28382.t1 AT1G79580 37.059 170 80 8 2 144 3 172 3.80e-22 92.0
MS.gene28382.t1 AT1G79580 37.059 170 80 8 2 144 3 172 3.80e-22 92.0
MS.gene28382.t1 AT1G79580 37.059 170 80 8 2 144 3 172 3.80e-22 92.0
MS.gene28382.t1 AT1G79580 37.059 170 80 8 2 144 3 172 3.80e-22 92.0
MS.gene28382.t1 AT5G53950 36.782 174 85 7 10 158 17 190 8.95e-22 90.9
MS.gene28382.t1 AT3G17730 37.419 155 75 6 6 138 2 156 1.04e-21 88.6
MS.gene28382.t1 AT4G27410 34.234 222 96 11 6 178 10 230 1.05e-21 90.1
MS.gene28382.t1 AT1G33280 37.179 156 78 6 4 139 2 157 1.22e-21 89.7
MS.gene28382.t1 AT3G10480 38.065 155 74 7 6 138 23 177 2.19e-21 90.5
MS.gene28382.t1 AT3G29035 42.188 128 58 4 8 119 22 149 2.65e-21 89.0
MS.gene28382.t1 AT3G10480 37.821 156 74 7 6 138 23 178 3.01e-21 90.1
MS.gene28382.t1 AT5G62380 34.591 159 81 7 5 141 2 159 3.28e-21 89.0
MS.gene28382.t1 AT5G62380 34.591 159 81 7 5 141 2 159 3.28e-21 89.0
MS.gene28382.t1 AT3G15170 37.255 153 72 5 10 138 20 172 3.77e-21 88.6
MS.gene28382.t1 AT1G76420 36.196 163 76 7 1 138 12 171 1.00e-20 87.4
MS.gene28382.t1 AT3G10490 34.839 155 78 7 7 138 24 178 2.12e-20 85.1
MS.gene28382.t1 AT5G17260 35.897 156 77 6 6 138 2 157 4.12e-20 87.0
MS.gene28382.t1 AT2G43000 32.051 156 85 5 12 146 20 175 8.24e-20 84.3
MS.gene28382.t1 AT2G18060 33.129 163 88 6 7 148 6 168 8.56e-20 85.5
MS.gene28382.t1 AT2G18060 33.129 163 88 6 7 148 6 168 8.56e-20 85.5
MS.gene28382.t1 AT4G36160 34.118 170 90 7 1 148 1 170 1.21e-19 85.1
MS.gene28382.t1 AT4G36160 34.118 170 90 7 1 148 1 170 1.21e-19 85.1
MS.gene28382.t1 AT4G36160 34.118 170 90 7 1 148 5 174 1.22e-19 85.1
MS.gene28382.t1 AT3G03200 35.714 154 76 6 8 138 4 157 1.27e-19 85.5
MS.gene28382.t1 AT1G52880 36.875 160 69 6 14 141 21 180 1.42e-19 84.3
MS.gene28382.t1 AT3G10490 34.839 155 78 7 7 138 24 178 1.57e-19 85.1
MS.gene28382.t1 AT1G65910 36.601 153 75 6 8 138 4 156 6.10e-19 83.6
MS.gene28382.t1 AT1G32770 31.707 164 79 6 9 139 15 178 9.77e-19 82.4
MS.gene28382.t1 AT5G04410 35.802 162 76 7 2 138 1 159 1.04e-18 83.2
MS.gene28382.t1 AT1G62700 35.484 155 77 7 9 141 6 159 2.39e-18 81.6
MS.gene28382.t1 AT1G62700 35.484 155 77 7 9 141 6 159 2.39e-18 81.6
MS.gene28382.t1 AT1G54330 34.868 152 80 6 8 140 4 155 3.03e-18 80.5
MS.gene28382.t1 AT3G61910 32.530 166 76 7 9 138 10 175 3.07e-18 80.9
MS.gene28382.t1 AT4G28530 32.727 165 75 5 9 138 9 172 6.26e-18 80.1
MS.gene28382.t1 AT1G12260 34.615 156 75 8 10 141 7 159 7.77e-18 79.7
MS.gene28382.t1 AT5G66300 33.333 159 80 7 7 141 9 165 9.51e-18 79.0
MS.gene28382.t1 AT3G10500 35.152 165 79 7 2 141 1 162 1.04e-17 80.1
MS.gene28382.t1 AT3G12910 31.250 176 82 9 5 143 17 190 1.05e-17 79.0
MS.gene28382.t1 AT1G12260 34.615 156 75 8 10 141 7 159 1.15e-17 79.7
MS.gene28382.t1 AT4G35580 34.161 161 80 7 2 138 1 159 2.21e-17 79.3
MS.gene28382.t1 AT4G17980 34.969 163 77 7 2 138 1 160 2.36e-17 77.4
MS.gene28382.t1 AT4G17980 34.969 163 77 7 2 138 1 160 2.48e-17 77.8
MS.gene28382.t1 AT4G35580 34.161 161 80 7 2 138 1 159 3.03e-17 79.0
MS.gene28382.t1 AT4G35580 34.161 161 80 7 2 138 1 159 3.03e-17 79.0
MS.gene28382.t1 AT1G71930 32.903 155 83 6 10 143 9 163 3.12e-17 77.8
MS.gene28382.t1 AT1G71930 32.903 155 83 6 10 143 9 163 3.12e-17 77.8
MS.gene28382.t1 AT5G46590 34.783 161 78 7 2 138 1 158 3.20e-17 77.4
MS.gene28382.t1 AT3G10480 32.275 189 72 7 6 138 23 211 3.84e-17 78.6
MS.gene28382.t1 AT1G33060 33.333 153 76 7 10 138 24 174 5.91e-17 78.2
MS.gene28382.t1 AT1G33060 33.333 153 76 7 10 138 24 174 6.14e-17 77.8
MS.gene28382.t1 AT5G39820 31.395 172 71 7 12 138 22 191 1.07e-16 76.6
MS.gene28382.t1 AT2G02450 34.228 149 73 6 12 138 53 198 1.79e-16 76.3
MS.gene28382.t1 AT1G26870 33.125 160 76 6 10 138 16 175 1.83e-16 76.3
MS.gene28382.t1 AT2G02450 34.228 149 73 6 12 138 53 198 2.24e-16 76.3
MS.gene28382.t1 AT2G17040 35.714 154 69 7 12 141 8 155 2.34e-16 75.1
MS.gene28382.t1 AT2G46770 29.885 174 80 7 9 143 15 185 6.17e-16 74.7
MS.gene28382.t1 AT5G46590 36.232 138 66 6 2 120 1 135 8.35e-16 73.6
MS.gene28382.t1 AT5G22290 29.221 154 90 5 12 148 23 174 1.13e-15 73.6
MS.gene28382.t1 AT1G02230 32.414 145 78 5 11 135 4 148 1.36e-15 73.6
MS.gene28382.t1 AT5G50820 34.058 138 73 4 6 128 13 147 8.22e-15 69.3
MS.gene28382.t1 AT1G32510 33.962 159 75 7 10 138 6 164 9.25e-15 70.5
MS.gene28382.t1 AT3G04420 33.108 148 78 6 11 138 4 150 3.54e-14 69.3
MS.gene28382.t1 AT5G14000 30.058 173 100 6 8 161 13 183 3.69e-14 67.8
MS.gene28382.t1 AT3G04420 33.103 145 76 6 11 135 4 147 4.27e-14 69.3
MS.gene28382.t1 AT1G34190 37.097 124 60 5 12 117 18 141 8.78e-14 68.9
MS.gene28382.t1 AT1G02250 30.345 145 81 4 11 135 4 148 9.55e-14 68.6
MS.gene28382.t1 AT4G01520 31.818 154 81 6 8 138 2 154 1.11e-13 67.8
MS.gene28382.t1 AT3G49530 29.167 168 92 7 10 152 13 178 1.36e-13 68.2
MS.gene28382.t1 AT4G01540 32.192 146 75 6 13 135 7 151 1.36e-13 68.2
MS.gene28382.t1 AT4G01540 32.215 149 77 6 13 138 7 154 1.77e-13 67.8
MS.gene28382.t1 AT4G01540 32.192 146 75 6 13 135 7 151 2.05e-13 67.8
MS.gene28382.t1 AT3G49530 29.167 168 92 7 10 152 13 178 2.15e-13 67.4
MS.gene28382.t1 AT4G01540 32.192 146 75 6 13 135 7 151 3.85e-13 66.6
MS.gene28382.t1 AT1G34180 34.921 126 64 5 12 119 18 143 3.50e-12 64.3
MS.gene28382.t1 AT1G34180 34.921 126 64 5 12 119 18 143 4.71e-12 63.5
MS.gene28382.t1 AT3G12977 33.913 115 51 5 58 152 14 123 6.21e-12 62.0
MS.gene28382.t1 AT2G33480 39.604 101 42 5 64 145 10 110 7.58e-12 61.6
MS.gene28382.t1 AT2G43000 32.609 92 50 3 67 146 6 97 4.31e-11 59.3
MS.gene28382.t1 AT5G61430 38.542 96 38 4 64 138 8 103 6.58e-11 59.7

Find 47 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAAACCTATAAATAAAT+TGG 0.152213 4.4:-9289763 MS.gene28382:CDS
TTCATTCCCAATTTATTTAT+AGG 0.164515 4.4:+9289756 None:intergenic
AATGGATATTGGAAGGAAAT+TGG 0.166312 4.4:-9289789 MS.gene28382:CDS
AAGCTAATTATTAACTAATT+AGG 0.204594 4.4:+9289330 None:intergenic
GAAAATAGAAGCACTGAAAA+TGG 0.301344 4.4:-9289807 MS.gene28382:CDS
GTGTGAGACAGGATCATAAT+TGG 0.363529 4.4:-9289500 MS.gene28382:intron
GAAGCACTGAAAATGGATAT+TGG 0.365813 4.4:-9289800 MS.gene28382:CDS
TTGATCAAGATCATCATCTA+AGG 0.370976 4.4:+9289362 None:intergenic
ATGGATATTGGAAGGAAATT+GGG 0.383014 4.4:-9289788 MS.gene28382:CDS
TGGTGAGACAAGTGAAGTTC+AGG 0.398752 4.4:+9290509 None:intergenic
CATGATCCTTGGGAAATATA+CGG 0.400748 4.4:-9290488 MS.gene28382:intron
AGTACCTTGTATTCAACCTT+GGG 0.405124 4.4:-9289731 MS.gene28382:CDS
ACTTGTCTCACCATGATCCT+TGG 0.405810 4.4:-9290499 MS.gene28382:CDS
GATGAGGATATTGATGATAA+TGG 0.421175 4.4:-9289420 MS.gene28382:CDS
CTTGTCTCACCATGATCCTT+GGG 0.421667 4.4:-9290498 MS.gene28382:CDS
GGTATGATGCTAGGATGATA+TGG 0.434256 4.4:+9290530 None:intergenic
CTTCCCCAAGGTTGAATACA+AGG 0.441845 4.4:+9289727 None:intergenic
AGCAGTGTGAAGCTTCCTCC+TGG 0.444718 4.4:-9290617 MS.gene28382:CDS
ACAAAACCTATAAATAAATT+GGG 0.452536 4.4:-9289762 MS.gene28382:CDS
AATTGAAACAAGTTGGGTAA+TGG 0.457936 4.4:-9289703 MS.gene28382:CDS
GGGAAGGAATTGAAACAAGT+TGG 0.476317 4.4:-9289710 MS.gene28382:CDS
ATGATATGGTAAAGAAGCCT+TGG 0.489756 4.4:+9290544 None:intergenic
CGTATATTTCCCAAGGATCA+TGG 0.491411 4.4:+9290489 None:intergenic
GGAAGGAATTGAAACAAGTT+GGG 0.495348 4.4:-9289709 MS.gene28382:CDS
ATATCATATTTCCTCATCTC+CGG 0.517814 4.4:-9289676 MS.gene28382:intron
AGGACTAGTTCTTCATCCGT+TGG 0.520052 4.4:+9290581 None:intergenic
AGGATCATAATTGGAGCAGA+TGG 0.520438 4.4:-9289491 MS.gene28382:CDS
TAATTATTAACTAATTAGGT+AGG 0.520877 4.4:+9289334 None:intergenic
GTACGTATATAAGAAATGAT+GGG 0.526478 4.4:-9290647 None:intergenic
AAGTACCTTGTATTCAACCT+TGG 0.541631 4.4:-9289732 MS.gene28382:CDS
CTTGTATTCAACCTTGGGGA+AGG 0.542660 4.4:-9289726 MS.gene28382:CDS
GCAGTGTGAAGCTTCCTCCT+GGG 0.560385 4.4:-9290616 MS.gene28382:CDS
TGAAGTTCAGGTATGATGCT+AGG 0.561302 4.4:+9290521 None:intergenic
TTGTTTCAATTCCTTCCCCA+AGG 0.571160 4.4:+9289715 None:intergenic
GGATCATAATTGGAGCAGAT+GGG 0.571776 4.4:-9289490 MS.gene28382:CDS
CCTTGGAATAGAGAAAATGA+AGG 0.572117 4.4:+9290561 None:intergenic
CACTGAAAATGGATATTGGA+AGG 0.575605 4.4:-9289796 MS.gene28382:CDS
GAAAAGAAGATGTCACATCA+AGG 0.579347 4.4:-9289450 MS.gene28382:CDS
TACGTATATAAGAAATGATG+GGG 0.594781 4.4:-9290646 None:intergenic
ACAATCACTTACCGGAGATG+AGG 0.598757 4.4:+9289665 None:intergenic
GACATACCGTATATTTCCCA+AGG 0.621341 4.4:+9290482 None:intergenic
ACAGGTAAAGCATTGTCAAG+TGG 0.622513 4.4:-9289861 MS.gene28382:intron
ACATCAAGGTGCAAGTGATG+AGG 0.626388 4.4:-9289436 MS.gene28382:CDS
GTACCTTGTATTCAACCTTG+GGG 0.661844 4.4:-9289730 MS.gene28382:CDS
ATAATGGAACAGATGTGTCA+TGG 0.669182 4.4:-9289404 MS.gene28382:CDS
GTTGGATCAAAGTAAAACCC+AGG 0.688035 4.4:+9290599 None:intergenic
GGATCAAAGTAAAACCCAGG+AGG 0.734981 4.4:+9290602 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTGGTATTATATATAT+AGG + chr4.4:9289642-9289661 None:intergenic 10.0%
!! ACAAAACCTATAAATAAATT+GGG - chr4.4:9290214-9290233 MS.gene28382:intron 15.0%
!! ATTCTAAATACGATTTAAAT+TGG - chr4.4:9289753-9289772 MS.gene28382:CDS 15.0%
!! TTCTAAATACGATTTAAATT+GGG - chr4.4:9289754-9289773 MS.gene28382:CDS 15.0%
!!! TTTTTTCCACATTAATTAAT+TGG + chr4.4:9290403-9290422 None:intergenic 15.0%
!! AAAAATATATCCGTTATTTG+TGG - chr4.4:9289993-9290012 MS.gene28382:intron 20.0%
!! AAATTTATGTAAAACCTATC+TGG - chr4.4:9290027-9290046 MS.gene28382:intron 20.0%
!! AGTGTTAAGACAAAATAAAT+TGG + chr4.4:9289657-9289676 None:intergenic 20.0%
!! ATTAGCAATAACATATGTTA+AGG + chr4.4:9289799-9289818 None:intergenic 20.0%
!! CACAAAACCTATAAATAAAT+TGG - chr4.4:9290213-9290232 MS.gene28382:intron 20.0%
!! TTCATTCCCAATTTATTTAT+AGG + chr4.4:9290223-9290242 None:intergenic 20.0%
!!! AACGGATATATTTTTTTACA+TGG + chr4.4:9289988-9290007 None:intergenic 20.0%
!!! ATTTGTTGTGATTTATGAAT+AGG + chr4.4:9290355-9290374 None:intergenic 20.0%
!!! CATAGCATATAATTGATTTT+TGG + chr4.4:9289856-9289875 None:intergenic 20.0%
!!! GATAGGTTTTACATAAATTT+TGG + chr4.4:9290027-9290046 None:intergenic 20.0%
! ATTCAATGATAGACTAATCT+CGG - chr4.4:9289905-9289924 MS.gene28382:intron 25.0%
! TATCTGGAAAATACTCTTTA+TGG - chr4.4:9290043-9290062 MS.gene28382:intron 25.0%
! TCATGACCAATTAATTAATG+TGG - chr4.4:9290394-9290413 MS.gene28382:intron 25.0%
! TCTATATATGCATGCATATA+TGG + chr4.4:9289607-9289626 None:intergenic 25.0%
!! GGATAATTTTCCACAAATAA+CGG + chr4.4:9290006-9290025 None:intergenic 25.0%
!!! AGCATATAATTGATTTTTGG+CGG + chr4.4:9289853-9289872 None:intergenic 25.0%
!!! ATAAAGAGTATTTTCCAGAT+AGG + chr4.4:9290044-9290063 None:intergenic 25.0%
AACAAAACACAATCACTTAC+CGG + chr4.4:9290322-9290341 None:intergenic 30.0%
AATGCTTTACCTGTTTACAA+AGG + chr4.4:9290109-9290128 None:intergenic 30.0%
AATGGATATTGGAAGGAAAT+TGG - chr4.4:9290187-9290206 MS.gene28382:intron 30.0%
ATAACTAAAGACAGTGAGAT+TGG + chr4.4:9289932-9289951 None:intergenic 30.0%
ATATACATGCCTTTGTAAAC+AGG - chr4.4:9290097-9290116 MS.gene28382:intron 30.0%
ATATCATATTTCCTCATCTC+CGG - chr4.4:9290300-9290319 MS.gene28382:intron 30.0%
ATGGATATTGGAAGGAAATT+GGG - chr4.4:9290188-9290207 MS.gene28382:intron 30.0%
ATTGATCAAGTGACATATAC+TGG - chr4.4:9289569-9289588 MS.gene28382:intron 30.0%
GAAAATACTCTTTATGGTTG+TGG - chr4.4:9290049-9290068 MS.gene28382:intron 30.0%
TTGATCAAGATCATCATCTA+AGG + chr4.4:9290617-9290636 None:intergenic 30.0%
! AAAGGCATGTATATTTCTTG+GGG + chr4.4:9290091-9290110 None:intergenic 30.0%
! AATTGAAACAAGTTGGGTAA+TGG - chr4.4:9290273-9290292 MS.gene28382:intron 30.0%
! ACAAAGGCATGTATATTTCT+TGG + chr4.4:9290093-9290112 None:intergenic 30.0%
! CAAAGGCATGTATATTTCTT+GGG + chr4.4:9290092-9290111 None:intergenic 30.0%
! GATGAGGATATTGATGATAA+TGG - chr4.4:9290556-9290575 MS.gene28382:CDS 30.0%
!! GAAAATAGAAGCACTGAAAA+TGG - chr4.4:9290169-9290188 MS.gene28382:intron 30.0%
!! TATTTTTGATTGTGTGAGAC+AGG - chr4.4:9290465-9290484 MS.gene28382:intron 30.0%
!!! CTTACTTTCTAGCTGAATTT+TGG + chr4.4:9289710-9289729 None:intergenic 30.0%
AAGGCATGTATATTTCTTGG+GGG + chr4.4:9290090-9290109 None:intergenic 35.0%
AAGTACCTTGTATTCAACCT+TGG - chr4.4:9290244-9290263 MS.gene28382:intron 35.0%
AGTACCTTGTATTCAACCTT+GGG - chr4.4:9290245-9290264 MS.gene28382:intron 35.0%
ATGATATGGTAAAGAAGCCT+TGG + chr4.4:9289435-9289454 None:intergenic 35.0%
CACTGAAAATGGATATTGGA+AGG - chr4.4:9290180-9290199 MS.gene28382:intron 35.0%
CATGATCCTTGGGAAATATA+CGG - chr4.4:9289488-9289507 MS.gene28382:CDS 35.0%
CCTTGGAATAGAGAAAATGA+AGG + chr4.4:9289418-9289437 None:intergenic 35.0%
GAAAAGAAGATGTCACATCA+AGG - chr4.4:9290526-9290545 MS.gene28382:CDS 35.0%
GGAAGGAATTGAAACAAGTT+GGG - chr4.4:9290267-9290286 MS.gene28382:intron 35.0%
! ATAATGGAACAGATGTGTCA+TGG - chr4.4:9290572-9290591 MS.gene28382:CDS 35.0%
! CCTTCATTTTCTCTATTCCA+AGG - chr4.4:9289415-9289434 MS.gene28382:CDS 35.0%
! GCTTCTATTTTCCTTCACTT+CGG + chr4.4:9290161-9290180 None:intergenic 35.0%
! TGGGTTTTACTTTGATCCAA+CGG - chr4.4:9289379-9289398 MS.gene28382:CDS 35.0%
!! GAAGCACTGAAAATGGATAT+TGG - chr4.4:9290176-9290195 MS.gene28382:intron 35.0%
ACGGTATGTCTTATAGTGCT+AGG - chr4.4:9289507-9289526 MS.gene28382:intron 40.0%
AGGATCATAATTGGAGCAGA+TGG - chr4.4:9290485-9290504 MS.gene28382:intron 40.0%
CGTATATTTCCCAAGGATCA+TGG + chr4.4:9289490-9289509 None:intergenic 40.0%
GACATACCGTATATTTCCCA+AGG + chr4.4:9289497-9289516 None:intergenic 40.0%
GGATCATAATTGGAGCAGAT+GGG - chr4.4:9290486-9290505 MS.gene28382:intron 40.0%
GGGAAGGAATTGAAACAAGT+TGG - chr4.4:9290266-9290285 MS.gene28382:intron 40.0%
GGTATGATGCTAGGATGATA+TGG + chr4.4:9289449-9289468 None:intergenic 40.0%
GTACCTTGTATTCAACCTTG+GGG - chr4.4:9290246-9290265 MS.gene28382:intron 40.0%
GTGTGAGACAGGATCATAAT+TGG - chr4.4:9290476-9290495 MS.gene28382:intron 40.0%
GTTGGATCAAAGTAAAACCC+AGG + chr4.4:9289380-9289399 None:intergenic 40.0%
TGAAGTTCAGGTATGATGCT+AGG + chr4.4:9289458-9289477 None:intergenic 40.0%
TTGTTTCAATTCCTTCCCCA+AGG + chr4.4:9290264-9290283 None:intergenic 40.0%
!! ACAGGTAAAGCATTGTCAAG+TGG - chr4.4:9290115-9290134 MS.gene28382:intron 40.0%
!!! CTACTTTTTCACCGAAGTGA+AGG - chr4.4:9290147-9290166 MS.gene28382:intron 40.0%
ACAATCACTTACCGGAGATG+AGG + chr4.4:9290314-9290333 None:intergenic 45.0%
ACATCAAGGTGCAAGTGATG+AGG - chr4.4:9290540-9290559 MS.gene28382:CDS 45.0%
ACTTGTCTCACCATGATCCT+TGG - chr4.4:9289477-9289496 MS.gene28382:CDS 45.0%
CTTCCCCAAGGTTGAATACA+AGG + chr4.4:9290252-9290271 None:intergenic 45.0%
CTTGTATTCAACCTTGGGGA+AGG - chr4.4:9290250-9290269 MS.gene28382:intron 45.0%
CTTGTCTCACCATGATCCTT+GGG - chr4.4:9289478-9289497 MS.gene28382:CDS 45.0%
GGATCAAAGTAAAACCCAGG+AGG + chr4.4:9289377-9289396 None:intergenic 45.0%
TGGTGAGACAAGTGAAGTTC+AGG + chr4.4:9289470-9289489 None:intergenic 45.0%
!! AGGACTAGTTCTTCATCCGT+TGG + chr4.4:9289398-9289417 None:intergenic 45.0%
AGCAGTGTGAAGCTTCCTCC+TGG - chr4.4:9289359-9289378 MS.gene28382:CDS 55.0%
GCAGTGTGAAGCTTCCTCCT+GGG - chr4.4:9289360-9289379 MS.gene28382:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 9289344 9290654 9289344 ID=MS.gene28382
chr4.4 mRNA 9289344 9290654 9289344 ID=MS.gene28382.t1;Parent=MS.gene28382
chr4.4 exon 9290489 9290654 9290489 ID=MS.gene28382.t1.exon1;Parent=MS.gene28382.t1
chr4.4 CDS 9290489 9290654 9290489 ID=cds.MS.gene28382.t1;Parent=MS.gene28382.t1
chr4.4 exon 9289677 9289879 9289677 ID=MS.gene28382.t1.exon2;Parent=MS.gene28382.t1
chr4.4 CDS 9289677 9289879 9289677 ID=cds.MS.gene28382.t1;Parent=MS.gene28382.t1
chr4.4 exon 9289344 9289511 9289344 ID=MS.gene28382.t1.exon3;Parent=MS.gene28382.t1
chr4.4 CDS 9289344 9289511 9289344 ID=cds.MS.gene28382.t1;Parent=MS.gene28382.t1
Gene Sequence

>MS.gene28382

ATGATGGGGAGTGAAAGCAGTGTGAAGCTTCCTCCTGGGTTTTACTTTGATCCAACGGATGAAGAACTAGTCCTTCATTTTCTCTATTCCAAGGCTTCTTTACCATATCATCCTAGCATCATACCTGAACTTCACTTGTCTCACCATGATCCTTGGGAAATATACGGTATGTCTTATAGTGCTAGGTATATTATATATATTTCATATAATATTTTCAATTTCAATATTGATCAAGTGACATATACTGGATATTTAGTTGACCATATATGCATGCATATATAGATAAAACTTCATTCCTATATATATAATACCAATTTATTTTGTCTTAACACTTTGATTCTCATTAAAATTAGCTCTCATAGTCCAAAATTCAGCTAGAAAGTAAGTAAGTAAAATCTAACAAAAAGATATTCTAAATACGATTTAAATTGGGATACTCTCAATCGATTTGACCTTAACATATGTTATTGCTAATTAAAAATATAGTTTGTGATATATGTAATCTTCCGCCAAAAATCAATTATATGCTATGAGATCTTTTTTTTTTTAAATTGAATATCGATTCAATGATAGACTAATCTCGGACCAATCTCACTGTCTTTAGTTATAGATTTATTATCTATCAAAGTTTTATCTGAAATCCATGTAAAAAAATATATCCGTTATTTGTGGAAAATTATCCAAAATTTATGTAAAACCTATCTGGAAAATACTCTTTATGGTTGTGGATGTGTATAATAACACCCCCAAGAAATATACATGCCTTTGTAAACAGGTAAAGCATTGTCAAGTGGAAATGAGCACTACTTTTTCACCGAAGTGAAGGAAAATAGAAGCACTGAAAATGGATATTGGAAGGAAATTGGGATCACAAAACCTATAAATAAATTGGGAATGAAGAAGTACCTTGTATTCAACCTTGGGGAAGGAATTGAAACAAGTTGGGTAATGGAAGAATATCATATTTCCTCATCTCCGGTAAGTGATTGTGTTTTGTTTTGTTAACTACCTATTCATAAATCACAACAAATTACTTTACTAAGAAAAAAATCATGACCAATTAATTAATGTGGAAAAAAATTAACATGAAGAACATAAAATTAAATTGATTTGTATCAATTTATTTTTGATTGTGTGAGACAGGATCATAATTGGAGCAGATGGGTTTTATGCAAAGTTTATGAAAAGAAGATGTCACATCAAGGTGCAAGTGATGAGGATATTGATGATAATGGAACAGATGTGTCATGGCTAGATGAAGTGTTTATGTCCTTAGATGATGATCTTGATCAAACAAGCCTACCTAATTAG
Protein sequence

>MS.gene28382.t1

MMGSESSVKLPPGFYFDPTDEELVLHFLYSKASLPYHPSIIPELHLSHHDPWEIYGKALSSGNEHYFFTEVKENRSTENGYWKEIGITKPINKLGMKKYLVFNLGEGIETSWVMEEYHISSSPDHNWSRWVLCKVYEKKMSHQGASDEDIDDNGTDVSWLDEVFMSLDDDLDQTSLPN