AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28393

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28393.t1 MTR_8g093580 94.903 412 19 2 1 412 80 489 0.0 789
MS.gene28393.t1 MTR_8g063550 67.813 407 82 7 1 406 80 438 0.0 514
MS.gene28393.t1 MTR_8g063550 75.667 300 67 4 1 299 80 374 2.34e-154 443
MS.gene28393.t1 MTR_8g063550 80.400 250 47 2 1 250 80 327 2.40e-140 404
MS.gene28393.t1 MTR_3g096140 59.355 155 58 2 1 150 81 235 2.53e-61 200
MS.gene28393.t1 MTR_3g096140 59.355 155 58 2 1 150 60 214 2.18e-60 199
MS.gene28393.t1 MTR_3g096140 59.355 155 58 2 1 150 81 235 3.37e-60 199
MS.gene28393.t1 MTR_1g090723 55.789 95 42 0 3 97 85 179 1.98e-36 141
MS.gene28393.t1 MTR_1g090723 57.895 95 39 1 3 97 85 178 6.44e-36 139
MS.gene28393.t1 MTR_1g090723 57.895 95 39 1 3 97 85 178 8.13e-36 139
MS.gene28393.t1 MTR_1g097300 56.000 100 44 0 3 102 95 194 6.85e-34 133
MS.gene28393.t1 MTR_0036s0150 52.577 97 45 1 3 99 82 177 3.18e-33 131
MS.gene28393.t1 MTR_6g477900 54.545 77 35 0 3 79 82 158 2.02e-25 103
MS.gene28393.t1 MTR_1g090720 48.246 114 52 2 1 108 116 228 1.00e-24 106
MS.gene28393.t1 MTR_2g062730 47.368 95 43 3 3 96 87 175 4.45e-23 99.8
MS.gene28393.t1 MTR_5g076850 46.591 88 46 1 3 89 82 169 7.94e-22 98.2
MS.gene28393.t1 MTR_4g108760 46.988 83 42 1 3 83 91 173 1.78e-21 95.5
MS.gene28393.t1 MTR_7g011130 39.423 104 47 2 3 90 93 196 2.15e-21 95.1
MS.gene28393.t1 MTR_3g070040 41.489 94 48 3 3 90 79 171 2.75e-21 94.4
MS.gene28393.t1 MTR_3g064580 46.591 88 45 2 3 88 82 169 3.05e-21 96.7
MS.gene28393.t1 MTR_6g084430 50.667 75 35 1 3 77 79 151 3.94e-21 94.0
MS.gene28393.t1 MTR_4g101680 43.220 118 58 5 3 117 84 195 4.34e-21 94.4
MS.gene28393.t1 MTR_3g435150 45.783 83 43 1 3 83 90 172 7.50e-21 92.8
MS.gene28393.t1 MTR_2g064090 52.632 76 35 1 3 77 82 157 1.60e-20 92.4
MS.gene28393.t1 MTR_4g035590 48.684 76 36 1 3 78 85 157 2.15e-20 92.0
MS.gene28393.t1 MTR_7g011120 39.796 98 49 2 3 90 82 179 2.19e-20 91.3
MS.gene28393.t1 MTR_8g024480 42.353 85 40 1 3 78 92 176 2.50e-20 92.8
MS.gene28393.t1 MTR_3g109340 45.783 83 42 2 3 83 96 177 3.42e-20 91.7
MS.gene28393.t1 MTR_3g109340 44.578 83 42 2 3 83 96 176 8.27e-20 90.5
MS.gene28393.t1 MTR_3g093050 48.052 77 40 0 1 77 96 172 9.68e-20 91.7
MS.gene28393.t1 MTR_3g093050 48.052 77 40 0 1 77 96 172 1.13e-19 91.7
MS.gene28393.t1 MTR_6g012670 40.000 95 51 2 3 93 94 186 1.13e-19 89.7
MS.gene28393.t1 MTR_1g096430 46.512 86 42 2 3 87 84 166 1.22e-19 89.7
MS.gene28393.t1 MTR_5g069030 46.154 78 42 0 1 78 99 176 1.23e-19 91.7
MS.gene28393.t1 MTR_4g036030 31.667 180 96 7 3 170 84 248 3.80e-19 88.6
MS.gene28393.t1 MTR_8g076110 48.684 76 37 1 3 78 83 156 4.14e-19 87.8
MS.gene28393.t1 MTR_2g078700 48.810 84 37 3 3 81 97 179 5.04e-19 88.6
MS.gene28393.t1 MTR_5g012080 48.101 79 35 3 3 78 84 159 2.04e-18 86.3
MS.gene28393.t1 MTR_7g005280 42.529 87 46 2 3 87 86 170 2.64e-18 84.3
MS.gene28393.t1 MTR_3g070030 39.362 94 49 3 3 90 93 184 3.30e-18 85.9
MS.gene28393.t1 MTR_2g064470 47.368 76 39 1 3 77 83 158 3.30e-18 85.1
MS.gene28393.t1 MTR_6g032770 48.750 80 35 3 3 77 92 170 3.97e-18 85.9
MS.gene28393.t1 MTR_5g021710 46.053 76 39 1 3 78 85 158 8.87e-18 84.3
MS.gene28393.t1 MTR_3g093040 45.570 79 39 2 1 77 182 258 8.97e-18 84.0
MS.gene28393.t1 MTR_1g008740 45.455 77 39 2 3 77 88 163 1.00e-17 84.3
MS.gene28393.t1 MTR_1g008740 45.455 77 39 2 3 77 102 177 1.52e-17 84.0
MS.gene28393.t1 MTR_2g079990 35.789 95 58 1 3 94 90 184 2.03e-17 83.6
MS.gene28393.t1 MTR_7g085260 45.783 83 35 3 3 77 91 171 3.06e-17 83.2
MS.gene28393.t1 MTR_4g081870 39.759 83 46 2 3 81 83 165 6.67e-17 80.5
MS.gene28393.t1 MTR_2g086880 43.836 73 34 1 2 74 92 157 1.16e-16 82.0
MS.gene28393.t1 MTR_2g086880 43.836 73 34 1 2 74 92 157 1.49e-16 82.0
MS.gene28393.t1 MTR_3g088110 42.500 80 42 2 3 79 81 159 2.96e-16 79.0
MS.gene28393.t1 MTR_4g098630 40.476 84 47 1 3 83 82 165 4.70e-16 79.0
MS.gene28393.t1 MTR_8g099750 38.542 96 48 3 1 89 84 175 8.05e-16 76.6
MS.gene28393.t1 MTR_2g080010 34.507 142 74 6 3 130 89 225 9.77e-16 78.6
MS.gene28393.t1 MTR_5g041940 40.000 80 44 2 3 78 77 156 1.30e-15 76.6
MS.gene28393.t1 MTR_4g075980 47.436 78 33 2 3 77 89 161 1.64e-15 77.0
MS.gene28393.t1 MTR_7g100990 37.363 91 50 3 3 87 85 174 1.91e-15 77.4
MS.gene28393.t1 MTR_2157s0010 36.957 92 46 2 3 82 82 173 2.23e-15 76.3
MS.gene28393.t1 MTR_8g094580 38.272 81 45 2 3 79 27 106 2.36e-15 75.5
MS.gene28393.t1 MTR_1g069805 45.455 77 36 2 3 77 84 156 2.45e-15 76.3
MS.gene28393.t1 MTR_4g134460 41.250 80 40 3 3 79 128 203 2.96e-15 77.4
MS.gene28393.t1 MTR_8g467490 39.535 86 41 3 3 77 88 173 3.73e-15 77.0
MS.gene28393.t1 MTR_8g059170 39.241 79 42 2 3 77 88 164 3.90e-15 76.3
MS.gene28393.t1 MTR_8g094580 38.272 81 45 2 3 79 81 160 5.04e-15 75.5
MS.gene28393.t1 MTR_7g085220 36.364 88 43 2 3 77 82 169 7.99e-15 75.5
MS.gene28393.t1 MTR_5g014300 37.500 104 55 2 3 99 82 182 1.17e-14 75.1
MS.gene28393.t1 MTR_3g116070 40.506 79 45 1 3 79 98 176 1.34e-14 75.1
MS.gene28393.t1 MTR_7g097090 44.737 76 36 2 3 76 84 155 1.42e-14 74.3
MS.gene28393.t1 MTR_6g011860 40.000 85 41 3 3 77 89 173 2.20e-14 74.7
MS.gene28393.t1 MTR_3g096920 38.750 80 45 2 3 79 81 159 4.47e-14 72.8
MS.gene28393.t1 MTR_5g040420 37.647 85 43 3 3 77 88 172 1.72e-13 72.0
MS.gene28393.t1 MTR_2g068880 36.667 90 42 2 3 77 97 186 4.44e-13 70.1
MS.gene28393.t1 MTR_3g098810 38.947 95 48 2 1 85 84 178 5.23e-13 68.9
MS.gene28393.t1 MTR_2g079990 44.262 61 34 0 3 63 90 150 6.19e-13 70.1
MS.gene28393.t1 MTR_1g097300 53.704 54 25 0 49 102 1 54 6.27e-13 70.5
MS.gene28393.t1 MTR_7g070140 52.727 55 24 1 2 54 37 91 7.80e-13 65.9
MS.gene28393.t1 MTR_2g093810 39.506 81 43 3 3 77 88 168 2.54e-12 67.8
MS.gene28393.t1 MTR_4g089135 51.852 54 24 1 3 54 89 142 8.06e-12 66.6
MS.gene28393.t1 MTR_7g070150 43.860 57 29 1 21 77 4 57 1.11e-11 61.2
MS.gene28393.t1 MTR_7g105170 32.927 82 49 2 2 77 83 164 2.66e-11 63.9
MS.gene28393.t1 MTR_8g023900 40.506 79 39 4 1 77 268 340 2.72e-11 65.5
MS.gene28393.t1 MTR_2g068920 50.000 56 24 1 3 54 96 151 2.78e-11 64.3
MS.gene28393.t1 MTR_3g098810 51.724 58 24 1 1 54 84 141 4.70e-11 61.6
MS.gene28393.t1 MTR_2g086690 35.802 81 48 3 3 79 84 164 4.79e-11 63.2
MS.gene28393.t1 MTR_5g090970 33.333 87 46 3 3 77 91 177 5.11e-11 63.9
MS.gene28393.t1 MTR_5g090970 33.333 87 46 3 3 77 92 178 5.15e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28393.t1 AT5G09330 47.429 175 78 3 3 165 82 254 1.23e-44 162
MS.gene28393.t1 AT5G09330 47.429 175 78 3 3 165 82 254 1.23e-44 162
MS.gene28393.t1 AT5G09330 47.429 175 78 3 3 165 82 254 1.23e-44 162
MS.gene28393.t1 AT5G09330 47.429 175 78 3 3 165 82 254 1.23e-44 162
MS.gene28393.t1 AT5G09330 47.429 175 78 3 3 165 82 254 1.23e-44 162
MS.gene28393.t1 AT5G64060 49.660 147 72 1 3 149 82 226 3.51e-42 152
MS.gene28393.t1 AT5G04410 52.174 115 50 1 3 112 85 199 1.60e-38 146
MS.gene28393.t1 AT3G10500 58.889 90 37 0 3 92 85 174 3.40e-35 137
MS.gene28393.t1 AT3G10490 52.941 102 46 2 1 101 101 201 1.22e-31 120
MS.gene28393.t1 AT3G10490 44.444 135 73 2 1 134 1 134 1.22e-31 123
MS.gene28393.t1 AT3G10490 44.444 135 73 2 1 134 1 134 1.22e-31 123
MS.gene28393.t1 AT1G34190 51.515 99 48 0 3 101 92 190 1.31e-31 126
MS.gene28393.t1 AT1G34180 45.872 109 53 1 3 105 92 200 2.95e-31 124
MS.gene28393.t1 AT1G34180 45.872 109 53 1 3 105 104 212 2.97e-31 124
MS.gene28393.t1 AT1G34180 45.872 109 53 1 3 105 92 200 6.96e-31 124
MS.gene28393.t1 AT1G34180 45.872 109 53 1 3 105 104 212 7.02e-31 124
MS.gene28393.t1 AT3G10490 44.776 134 72 2 1 133 101 233 8.00e-31 123
MS.gene28393.t1 AT3G10480 47.899 119 59 3 1 117 101 218 1.17e-29 120
MS.gene28393.t1 AT3G10480 47.458 118 60 2 1 117 101 217 1.22e-29 119
MS.gene28393.t1 AT3G10480 52.000 100 46 2 1 99 134 232 1.95e-29 120
MS.gene28393.t1 AT1G32870 53.261 92 41 1 3 92 84 175 1.64e-27 114
MS.gene28393.t1 AT1G32870 53.261 92 41 1 3 92 84 175 1.72e-27 114
MS.gene28393.t1 AT1G32870 53.261 92 41 1 3 92 120 211 2.68e-27 114
MS.gene28393.t1 AT3G17730 55.844 77 34 0 3 79 82 158 1.61e-25 104
MS.gene28393.t1 AT3G12977 51.852 81 38 1 3 82 39 119 2.89e-24 100
MS.gene28393.t1 AT1G33060 53.333 75 35 0 3 77 100 174 1.13e-23 103
MS.gene28393.t1 AT1G33060 53.333 75 35 0 3 77 100 174 1.15e-23 103
MS.gene28393.t1 AT1G65910 47.674 86 45 0 3 88 82 167 1.96e-23 103
MS.gene28393.t1 AT3G12977 52.500 80 37 1 3 81 92 171 2.42e-23 99.4
MS.gene28393.t1 AT5G61430 49.398 83 40 1 3 83 27 109 9.94e-23 97.4
MS.gene28393.t1 AT4G10350 50.000 76 38 0 3 78 39 114 1.63e-22 97.1
MS.gene28393.t1 AT5G61430 49.398 83 40 1 3 83 90 172 3.41e-22 97.1
MS.gene28393.t1 AT4G10350 50.000 76 38 0 3 78 86 161 3.68e-22 97.1
MS.gene28393.t1 AT1G71930 46.914 81 41 1 3 83 86 164 4.82e-22 96.3
MS.gene28393.t1 AT1G71930 46.914 81 41 1 3 83 86 164 4.82e-22 96.3
MS.gene28393.t1 AT5G39610 48.193 83 41 1 3 83 94 176 2.71e-21 93.6
MS.gene28393.t1 AT1G33280 47.368 76 37 1 3 78 85 157 1.74e-20 91.7
MS.gene28393.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.86e-20 92.4
MS.gene28393.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.86e-20 92.4
MS.gene28393.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.86e-20 92.4
MS.gene28393.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.86e-20 92.4
MS.gene28393.t1 AT1G79580 48.052 77 36 2 3 78 94 167 1.86e-20 92.4
MS.gene28393.t1 AT4G35580 49.333 75 38 0 3 77 85 159 2.67e-20 93.2
MS.gene28393.t1 AT4G35580 49.333 75 38 0 3 77 85 159 2.67e-20 93.6
MS.gene28393.t1 AT4G35580 49.333 75 38 0 3 77 85 159 2.73e-20 93.2
MS.gene28393.t1 AT1G12260 40.187 107 58 3 3 108 84 185 2.82e-20 92.4
MS.gene28393.t1 AT5G07680 45.783 83 43 1 3 83 77 159 3.13e-20 91.3
MS.gene28393.t1 AT1G12260 40.187 107 58 3 3 108 84 185 3.13e-20 91.7
MS.gene28393.t1 AT5G07680 45.783 83 43 1 3 83 91 173 4.23e-20 90.9
MS.gene28393.t1 AT1G62700 38.136 118 62 5 3 117 84 193 4.74e-20 91.7
MS.gene28393.t1 AT1G62700 38.136 118 62 5 3 117 84 193 4.74e-20 91.7
MS.gene28393.t1 AT1G69490 30.303 165 102 3 3 156 83 245 5.74e-20 89.4
MS.gene28393.t1 AT1G76420 51.899 79 35 2 3 79 96 173 5.77e-20 90.5
MS.gene28393.t1 AT5G24590 46.835 79 41 1 1 78 87 165 7.43e-20 91.7
MS.gene28393.t1 AT2G27300 47.945 73 31 1 2 74 88 153 8.17e-20 90.1
MS.gene28393.t1 AT1G56010 42.105 95 52 1 3 94 27 121 8.59e-20 88.6
MS.gene28393.t1 AT3G18400 43.210 81 45 1 3 83 79 158 1.14e-19 89.4
MS.gene28393.t1 AT3G49530 41.667 108 52 2 3 109 89 186 1.56e-19 90.5
MS.gene28393.t1 AT3G49530 41.667 108 52 2 3 109 89 186 1.61e-19 90.9
MS.gene28393.t1 AT1G56010 42.105 95 52 1 3 94 94 188 2.36e-19 88.6
MS.gene28393.t1 AT2G18060 46.835 79 40 1 3 81 86 162 4.08e-19 88.6
MS.gene28393.t1 AT2G18060 46.835 79 40 1 3 81 86 162 4.08e-19 88.6
MS.gene28393.t1 AT4G36160 43.750 96 45 2 3 98 91 177 4.38e-19 88.6
MS.gene28393.t1 AT4G36160 43.750 96 45 2 3 98 87 173 4.43e-19 88.6
MS.gene28393.t1 AT4G36160 43.750 96 45 2 3 98 87 173 4.43e-19 88.6
MS.gene28393.t1 AT2G46770 40.449 89 40 2 3 78 93 181 6.01e-19 88.2
MS.gene28393.t1 AT5G13180 48.810 84 40 2 3 84 85 167 6.03e-19 86.3
MS.gene28393.t1 AT2G24430 48.052 77 37 2 3 77 90 165 6.17e-19 87.4
MS.gene28393.t1 AT2G24430 48.052 77 37 2 3 77 90 165 6.17e-19 87.4
MS.gene28393.t1 AT5G18270 48.193 83 36 3 3 80 95 175 6.92e-19 87.4
MS.gene28393.t1 AT5G18270 48.193 83 36 3 3 80 95 175 7.83e-19 87.4
MS.gene28393.t1 AT5G17260 47.368 76 39 1 3 77 82 157 1.00e-18 88.6
MS.gene28393.t1 AT3G29035 42.857 91 50 1 3 91 98 188 1.19e-18 86.7
MS.gene28393.t1 AT3G03200 47.368 76 39 1 3 77 82 157 1.33e-18 88.2
MS.gene28393.t1 AT3G04060 46.914 81 40 2 3 80 94 174 1.85e-18 86.3
MS.gene28393.t1 AT2G18060 41.758 91 43 2 8 98 3 83 3.25e-18 84.7
MS.gene28393.t1 AT5G22290 44.595 74 33 2 2 74 95 161 5.50e-18 85.1
MS.gene28393.t1 AT3G61910 40.909 88 39 1 3 77 88 175 5.89e-18 84.7
MS.gene28393.t1 AT5G53950 48.750 80 35 3 3 77 91 169 7.75e-18 85.1
MS.gene28393.t1 AT5G62380 46.053 76 39 1 3 78 84 157 8.37e-18 84.7
MS.gene28393.t1 AT5G62380 46.053 76 39 1 3 78 84 157 8.37e-18 84.7
MS.gene28393.t1 AT3G44290 43.836 73 34 1 2 74 88 153 8.52e-18 82.4
MS.gene28393.t1 AT3G15170 45.349 86 41 3 3 83 94 178 2.08e-17 82.8
MS.gene28393.t1 AT3G44290 43.836 73 34 1 2 74 88 153 3.28e-17 82.8
MS.gene28393.t1 AT1G54330 46.341 82 41 1 3 84 82 160 3.65e-17 82.0
MS.gene28393.t1 AT1G52890 35.789 95 57 1 3 93 88 182 6.69e-17 81.6
MS.gene28393.t1 AT2G33480 46.053 76 41 0 3 78 29 104 1.16e-16 79.0
MS.gene28393.t1 AT2G33480 46.053 76 39 1 3 78 86 159 1.24e-16 79.7
MS.gene28393.t1 AT3G15500 38.158 76 47 0 3 78 88 163 1.78e-16 80.1
MS.gene28393.t1 AT1G32770 38.372 86 43 1 3 78 93 178 2.08e-16 80.5
MS.gene28393.t1 AT1G01720 36.036 111 62 3 3 105 81 190 2.60e-16 79.3
MS.gene28393.t1 AT4G27410 38.667 75 46 0 3 77 88 162 2.66e-16 79.3
MS.gene28393.t1 AT2G33480 46.053 76 41 0 3 78 86 161 2.88e-16 79.0
MS.gene28393.t1 AT5G66300 41.111 90 50 2 3 91 89 176 3.20e-16 79.0
MS.gene28393.t1 AT4G27410 38.667 75 46 0 3 77 105 179 3.21e-16 79.3
MS.gene28393.t1 AT5G22290 42.254 71 33 2 7 76 1 64 3.47e-16 78.2
MS.gene28393.t1 AT5G46590 42.857 77 42 2 3 77 82 158 5.87e-16 78.2
MS.gene28393.t1 AT1G77450 41.250 80 43 2 3 79 84 162 1.16e-15 76.6
MS.gene28393.t1 AT5G08790 39.024 82 44 2 3 79 81 161 1.83e-15 76.6
MS.gene28393.t1 AT4G28530 46.753 77 35 2 3 77 100 172 1.93e-15 77.4
MS.gene28393.t1 AT4G17980 43.038 79 41 2 3 77 82 160 3.36e-15 75.5
MS.gene28393.t1 AT4G17980 43.038 79 41 2 3 77 82 160 4.37e-15 75.9
MS.gene28393.t1 AT5G63790 39.024 82 44 2 3 79 134 214 4.45e-15 76.3
MS.gene28393.t1 AT5G63790 39.024 82 44 2 3 79 124 204 4.81e-15 75.9
MS.gene28393.t1 AT3G15510 42.045 88 38 4 3 77 91 178 1.55e-14 75.1
MS.gene28393.t1 AT1G61110 38.636 88 41 2 3 77 90 177 1.90e-14 74.3
MS.gene28393.t1 AT1G32510 38.824 85 44 2 1 77 80 164 2.26e-14 73.6
MS.gene28393.t1 AT5G46590 49.153 59 29 1 3 60 82 140 2.66e-14 73.2
MS.gene28393.t1 AT2G02450 39.744 78 44 2 3 79 125 200 5.54e-14 73.6
MS.gene28393.t1 AT2G02450 39.744 78 44 2 3 79 125 200 8.20e-14 73.2
MS.gene28393.t1 AT2G43000 39.474 76 45 1 3 77 14 89 1.10e-13 69.7
MS.gene28393.t1 AT2G17040 41.026 78 38 2 3 77 80 152 1.44e-13 71.2
MS.gene28393.t1 AT3G04070 38.636 88 40 3 3 77 84 170 1.79e-13 71.6
MS.gene28393.t1 AT4G01540 45.000 80 36 2 1 77 80 154 2.36e-13 71.6
MS.gene28393.t1 AT1G26870 38.372 86 42 3 3 77 90 175 2.72e-13 71.6
MS.gene28393.t1 AT4G01540 45.000 80 36 2 1 77 80 154 2.72e-13 71.2
MS.gene28393.t1 AT4G01520 43.750 80 37 2 1 77 80 154 3.63e-13 70.1
MS.gene28393.t1 AT4G01540 45.000 80 36 2 1 77 80 154 3.84e-13 71.2
MS.gene28393.t1 AT4G01540 45.000 80 36 2 1 77 80 154 4.27e-13 70.9
MS.gene28393.t1 AT2G43000 39.474 76 45 1 3 77 92 167 5.41e-13 69.3
MS.gene28393.t1 AT5G04395 50.820 61 27 1 1 58 113 173 7.49e-13 67.4
MS.gene28393.t1 AT3G04070 30.097 103 44 2 3 77 84 186 5.94e-12 67.0
MS.gene28393.t1 AT4G01550 40.964 83 33 3 2 77 80 153 1.04e-11 66.6
MS.gene28393.t1 AT1G52880 39.080 87 41 3 3 77 91 177 7.21e-11 63.5

Find 111 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTCGACTCCAACTTTC+AGG 0.173690 4.4:-9177498 None:intergenic
ATGAAACGAGCTACGGAAAT+TGG 0.193778 4.4:+9175599 MS.gene28393:CDS
CCTAGGAATGGTGCGCAATA+TGG 0.233667 4.4:+9176296 MS.gene28393:CDS
TTTCAAACTAGTGGATATAT+TGG 0.258960 4.4:+9176440 MS.gene28393:CDS
TTATTAACAGTTTGATTGTT+TGG 0.271416 4.4:-9176977 None:intergenic
CGTGGATTGCTCGGCTGAAT+TGG 0.273008 4.4:+9177347 MS.gene28393:CDS
CCTGTTTGACATGGATTTGC+AGG 0.278572 4.4:-9176551 None:intergenic
CCTGTTTGACAAGAATTAAC+AGG 0.308110 4.4:-9176593 None:intergenic
CTCCAGTCTTCCTCATTAAA+TGG 0.323348 4.4:-9176323 None:intergenic
CTTGCGAGTTCATGTCAAAT+AGG 0.339836 4.4:+9176806 MS.gene28393:CDS
GAGCTACGGAAATTGGGTTT+TGG 0.345895 4.4:+9175606 MS.gene28393:CDS
TTATTAACAGCTTGATTGTC+TGG 0.351063 4.4:-9176866 None:intergenic
TTTGGATATGAATCATTTGC+AGG 0.354641 4.4:-9176959 None:intergenic
TCTGTTTGACATGAATTTGC+AGG 0.364697 4.4:-9176764 None:intergenic
CCTGTTTGACATGAATTTGC+TGG 0.368512 4.4:-9176638 None:intergenic
TCTGGATATGAATCATTTGC+AGG 0.389690 4.4:-9176848 None:intergenic
GCATGACGACTTCGATTTCA+TGG 0.391062 4.4:+9177434 MS.gene28393:CDS
CCTGTTAATTCTTGTCAAAC+AGG 0.394873 4.4:+9176593 MS.gene28393:CDS
CACACACGGACTGGCTTCTA+CGG 0.395311 4.4:+9177398 MS.gene28393:CDS
TGACAAGAATTCACAGGGTC+TGG 0.397096 4.4:-9176716 None:intergenic
CCTGTGAATTCTTGTCAAAC+AGG 0.404297 4.4:+9176722 MS.gene28393:CDS
TGACAAGAATTAACAGGGTC+TGG 0.407202 4.4:-9176587 None:intergenic
TGACATGGATTTGCAGGGTC+AGG 0.408876 4.4:-9176545 None:intergenic
GCTTCTACGGGCAATGTTGT+GGG 0.411525 4.4:+9177411 MS.gene28393:CDS
ATTAGATGTCACAGGCAAAC+TGG 0.412373 4.4:-9176393 None:intergenic
TCGACTCCAACTTTCAGGTT+GGG 0.415732 4.4:-9177493 None:intergenic
ATCAGAATTGGTGCCTTCTC+CGG 0.423053 4.4:+9176484 MS.gene28393:CDS
CTGTTTGACATGAATTTGTT+GGG 0.425559 4.4:-9176508 None:intergenic
TGACATGAACTCGCAAGGTC+AGG 0.427713 4.4:-9176800 None:intergenic
CCAACAAATTCATGTCAAAC+AGG 0.430400 4.4:+9176509 MS.gene28393:CDS
GTGGATTGCTCGGCTGAATT+GGG 0.430538 4.4:+9177348 MS.gene28393:CDS
GGCTTCTACGGGCAATGTTG+TGG 0.430701 4.4:+9177410 MS.gene28393:CDS
CCTGTTTGACATGAATTTGT+TGG 0.430797 4.4:-9176509 None:intergenic
TGAAACGAGCTACGGAAATT+GGG 0.444296 4.4:+9175600 MS.gene28393:CDS
CCTGCAAATCCATGTCAAAC+AGG 0.444856 4.4:+9176551 MS.gene28393:CDS
GGCTGACAAAGGAATTGTTC+AGG 0.445801 4.4:+9175775 MS.gene28393:CDS
CCATATTGCGCACCATTCCT+AGG 0.447039 4.4:-9176296 None:intergenic
GTGTGCTAAAGTCTGCATTC+TGG 0.450394 4.4:-9177380 None:intergenic
CTAGGAATGGTGCGCAATAT+GGG 0.452600 4.4:+9176297 MS.gene28393:CDS
TGACATGAATTTGTTGGGTC+CGG 0.453148 4.4:-9176503 None:intergenic
ATCTAGACTCTGATACGTTC+TGG 0.454030 4.4:+9177466 MS.gene28393:CDS
TCGGCTGAATTGGGATCTGC+TGG 0.459546 4.4:+9177357 MS.gene28393:CDS
TGAGTATAGGCTTGAAGATA+AGG 0.460176 4.4:+9175748 MS.gene28393:CDS
CCAGCAAATTCATGTCAAAC+AGG 0.464214 4.4:+9176638 MS.gene28393:CDS
GAATTCACAGGGTCTGGATA+AGG 0.466095 4.4:-9176710 None:intergenic
TGAAAAGAATGTAAAGGGTC+AGG 0.467715 4.4:-9176674 None:intergenic
TATGAATCATTTGCAGGGTC+AGG 0.468577 4.4:-9176842 None:intergenic
TATGAATCATTTGCAGGGTC+GGG 0.476385 4.4:-9176953 None:intergenic
CATGCCGTGTCAATCAGAAT+TGG 0.476656 4.4:+9176472 MS.gene28393:CDS
GAATTAACAGGGTCTGGATA+AGG 0.476908 4.4:-9176581 None:intergenic
TCATTTGCAGGGTCGGGCGC+AGG 0.479506 4.4:-9176947 None:intergenic
ATTGGGTTATGCATGAGTAT+AGG 0.479985 4.4:+9175735 MS.gene28393:CDS
AGTGCAGCCGATTCCGCAAA+AGG 0.480373 4.4:-9176365 None:intergenic
GAATTTGCAGGGTCGGAAGC+AGG 0.480953 4.4:-9176752 None:intergenic
TGACATGAATTTGCTGGGTC+GGG 0.482049 4.4:-9176632 None:intergenic
TTCGACTCCAACTTTCAGGT+TGG 0.483370 4.4:-9177494 None:intergenic
CTGTTTGAAAAGAATGTAAA+GGG 0.489842 4.4:-9176679 None:intergenic
GACCATTTAATGAGGAAGAC+TGG 0.495616 4.4:+9176321 MS.gene28393:CDS
TCGTGGTGATCGAACCGATT+GGG 0.500477 4.4:+9175718 MS.gene28393:CDS
GGCAGACCCAACCTGAAAGT+TGG 0.501795 4.4:+9177487 MS.gene28393:CDS
GAATTTGTTGGGTCCGGAGA+AGG 0.503131 4.4:-9176497 None:intergenic
CCTGTTTGAAAAGAATGTAA+AGG 0.504024 4.4:-9176680 None:intergenic
CTGTTTGACATGAATTTGCT+GGG 0.507084 4.4:-9176637 None:intergenic
CCTGTTTGACAAGAATTCAC+AGG 0.507170 4.4:-9176722 None:intergenic
AGACTTTAGCACACACGGAC+TGG 0.509776 4.4:+9177389 MS.gene28393:CDS
TTGACATGAATTTGCTGGGT+CGG 0.510788 4.4:-9176633 None:intergenic
ATATGAATCATTTGCAGGGT+CGG 0.522037 4.4:-9176954 None:intergenic
GAATTTGCTGGGTCGGGAGA+CGG 0.524781 4.4:-9176626 None:intergenic
CTGTTTGACATGAATTTGCA+GGG 0.530109 4.4:-9176763 None:intergenic
CTCGTGGTGATCGAACCGAT+TGG 0.531457 4.4:+9175717 MS.gene28393:CDS
GGATTCCTACGTAATCTGTA+AGG 0.535028 4.4:+9176250 MS.gene28393:intron
GAATGTAAAGGGTCAGGAGA+AGG 0.535783 4.4:-9176668 None:intergenic
GACATGAACTCGCAAGGTCA+GGG 0.545876 4.4:-9176799 None:intergenic
CTGTTTGACATGGATTTGCA+GGG 0.546270 4.4:-9176550 None:intergenic
TAGGCTTGAAGATAAGGACT+TGG 0.547580 4.4:+9175754 MS.gene28393:CDS
CAACAGAATAATCAAACGGT+GGG 0.550179 4.4:+9175656 MS.gene28393:CDS
CAATTCTGATTGACACGGCA+TGG 0.550897 4.4:-9176471 None:intergenic
ATACTCATGCATAACCCAAT+CGG 0.550912 4.4:-9175732 None:intergenic
TTGACATGAATTTGCAGGGT+CGG 0.556895 4.4:-9176759 None:intergenic
GGCATAAATCCTGTTTGACA+TGG 0.562278 4.4:-9176560 None:intergenic
CTTGGAAGACGTGGATTGCT+CGG 0.562682 4.4:+9177338 MS.gene28393:CDS
ACACACGGACTGGCTTCTAC+GGG 0.563688 4.4:+9177399 MS.gene28393:CDS
GACTGGAGTGATGATGAAGT+AGG 0.564465 4.4:+9176338 MS.gene28393:CDS
GGATTTGCAGGGTCAGGAGA+AGG 0.569179 4.4:-9176539 None:intergenic
GATAAGGACTTGGCTGACAA+AGG 0.573980 4.4:+9175764 MS.gene28393:CDS
GTTAATTCTTGTCAAACAGG+AGG 0.576417 4.4:+9176596 MS.gene28393:CDS
ACAGAACAATTAGATGTCAC+AGG 0.589124 4.4:-9176401 None:intergenic
GAAGGTCCTGGTCCTAGGAA+TGG 0.591114 4.4:+9176284 MS.gene28393:CDS
CTGTTTGACAAGAATTCACA+GGG 0.591836 4.4:-9176721 None:intergenic
CTTCTACGGGCAATGTTGTG+GGG 0.592770 4.4:+9177412 MS.gene28393:CDS
TTGGATATGAATCATTTGCA+GGG 0.592806 4.4:-9176958 None:intergenic
TCATTTGCAGGGTCAGGCAA+AGG 0.595550 4.4:-9176836 None:intergenic
TGTTCAACAGAATAATCAAA+CGG 0.610587 4.4:+9175652 MS.gene28393:CDS
CTGGATATGAATCATTTGCA+GGG 0.611969 4.4:-9176847 None:intergenic
TGCGCACCATTCCTAGGACC+AGG 0.613457 4.4:-9176290 None:intergenic
CTATTTGACATGAACTCGCA+AGG 0.614626 4.4:-9176805 None:intergenic
CTGTTTGACAAGAATTAACA+GGG 0.620690 4.4:-9176592 None:intergenic
AAAACCCTAATATTTCACAC+TGG 0.629779 4.4:+9175686 MS.gene28393:CDS
GCCAAGGATGAAACGAGCTA+CGG 0.632310 4.4:+9175592 None:intergenic
ATATGGGAGACCATTTAATG+AGG 0.637886 4.4:+9176313 MS.gene28393:CDS
ACATGAACTCGCAAGGTCAG+GGG 0.640717 4.4:-9176798 None:intergenic
AATGCAGACTTTAGCACACA+CGG 0.659462 4.4:+9177384 MS.gene28393:CDS
GGCACCAATTCTGATTGACA+CGG 0.665036 4.4:-9176476 None:intergenic
AACAGAATAATCAAACGGTG+GGG 0.675165 4.4:+9175657 MS.gene28393:CDS
AAATACCTTACAGATTACGT+AGG 0.675415 4.4:-9176255 None:intergenic
GAACTCGCAAGGTCAGGGGA+CGG 0.679790 4.4:-9176794 None:intergenic
TCAACAGAATAATCAAACGG+TGG 0.680625 4.4:+9175655 MS.gene28393:CDS
TGTCAGGAAAACATTGCTGA+AGG 0.693858 4.4:+9177288 MS.gene28393:intron
AGACAACGGTGTATTGCCTG+AGG 0.701304 4.4:+9177039 MS.gene28393:CDS
CACACTGGAAAATCACCTCG+TGG 0.720810 4.4:+9175701 MS.gene28393:CDS
CAATCGGTTCGATCACCACG+AGG 0.739524 4.4:-9175716 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAAAAGAAAGCAA+GGG - chr4.4:9176167-9176186 None:intergenic 15.0%
!! AATAACATAATTCTATTTCA+AGG - chr4.4:9175986-9176005 None:intergenic 15.0%
!! ATAGAATTATGTTATTACAT+TGG + chr4.4:9175990-9176009 MS.gene28393:intron 15.0%
!! AGTAAAAAATGCACTTTATA+AGG - chr4.4:9176043-9176062 None:intergenic 20.0%
!! CAAATAATTAAAAGAAAGCA+AGG - chr4.4:9176168-9176187 None:intergenic 20.0%
!! TTATTAACAGTTTGATTGTT+TGG - chr4.4:9176980-9176999 None:intergenic 20.0%
!!! TGTTTTATATGAGCTTTATA+TGG + chr4.4:9175817-9175836 MS.gene28393:intron 20.0%
!!! TTTAAAGTAGTTGTAATATG+TGG - chr4.4:9175863-9175882 None:intergenic 20.0%
! ATCGCTATGATTTAGTAAAA+TGG - chr4.4:9177215-9177234 None:intergenic 25.0%
! CTGTTTGAAAAGAATGTAAA+GGG - chr4.4:9176682-9176701 None:intergenic 25.0%
! TACAACTACTTTAAATGCTA+GGG + chr4.4:9175868-9175887 MS.gene28393:intron 25.0%
! TGTTCAACAGAATAATCAAA+CGG + chr4.4:9175652-9175671 MS.gene28393:CDS 25.0%
! TTACAACTACTTTAAATGCT+AGG + chr4.4:9175867-9175886 MS.gene28393:intron 25.0%
!! ATTTTCCAGTGTGAAATATT+AGG - chr4.4:9175694-9175713 None:intergenic 25.0%
!! CTTGATATTTTCAAAGACAA+CGG + chr4.4:9176917-9176936 MS.gene28393:CDS 25.0%
!! TGTAAGGTATTTCAAAAAGA+AGG + chr4.4:9176266-9176285 MS.gene28393:CDS 25.0%
!! TTTCAAACTAGTGGATATAT+TGG + chr4.4:9176440-9176459 MS.gene28393:CDS 25.0%
!! TTTTCCAGTGTGAAATATTA+GGG - chr4.4:9175693-9175712 None:intergenic 25.0%
!!! ATGTTTTTAACAAGTTCTCT+TGG + chr4.4:9176220-9176239 MS.gene28393:intron 25.0%
!!! CTTGATATTTTTGAAGACAA+CGG + chr4.4:9177025-9177044 MS.gene28393:CDS 25.0%
!!! TGATGTTTCTTTTTTATGTC+TGG + chr4.4:9177113-9177132 MS.gene28393:intron 25.0%
AAAACCCTAATATTTCACAC+TGG + chr4.4:9175686-9175705 MS.gene28393:CDS 30.0%
AAATACCTTACAGATTACGT+AGG - chr4.4:9176258-9176277 None:intergenic 30.0%
AGTTATCCTTCAAGAAATCA+AGG - chr4.4:9177247-9177266 None:intergenic 30.0%
CCTGTTTGAAAAGAATGTAA+AGG - chr4.4:9176683-9176702 None:intergenic 30.0%
CTGTTTGACAAGAATTAACA+GGG - chr4.4:9176595-9176614 None:intergenic 30.0%
TACTTTAAATGCTAGGGTTA+GGG + chr4.4:9175874-9175893 MS.gene28393:intron 30.0%
TATAGTCCTTGATTTCTTGA+AGG + chr4.4:9177238-9177257 MS.gene28393:intron 30.0%
TCCAGAAAATCACAAGTAAA+AGG - chr4.4:9177086-9177105 None:intergenic 30.0%
TTATTAACAGCTTGATTGTC+TGG - chr4.4:9176869-9176888 None:intergenic 30.0%
TTGGATATGAATCATTTGCA+GGG - chr4.4:9176961-9176980 None:intergenic 30.0%
TTTGGATATGAATCATTTGC+AGG - chr4.4:9176962-9176981 None:intergenic 30.0%
! CCTTTACATTCTTTTCAAAC+AGG + chr4.4:9176680-9176699 MS.gene28393:CDS 30.0%
! CTGTTTGACATGAATTTGTT+GGG - chr4.4:9176511-9176530 None:intergenic 30.0%
! GATCAGAATTTTCAAACTAG+TGG + chr4.4:9176431-9176450 MS.gene28393:CDS 30.0%
! TCCTTTTACTTGTGATTTTC+TGG + chr4.4:9177082-9177101 MS.gene28393:intron 30.0%
!! CTGAGTTTTGACATTGTTAT+TGG + chr4.4:9177265-9177284 MS.gene28393:intron 30.0%
!!! CTCTTAACTGACTTTTTTGA+TGG + chr4.4:9177315-9177334 MS.gene28393:CDS 30.0%
AAAACAGATTAGCATACCTC+AGG - chr4.4:9177058-9177077 None:intergenic 35.0%
AAAATGGCATCAACAGTATG+AGG - chr4.4:9177199-9177218 None:intergenic 35.0%
AACAGAATAATCAAACGGTG+GGG + chr4.4:9175657-9175676 MS.gene28393:CDS 35.0%
AACATATTCGCAACGAGAAT+AGG - chr4.4:9176205-9176224 None:intergenic 35.0%
ACAGAACAATTAGATGTCAC+AGG - chr4.4:9176404-9176423 None:intergenic 35.0%
ATACTCATGCATAACCCAAT+CGG - chr4.4:9175735-9175754 None:intergenic 35.0%
ATATGAATCATTTGCAGGGT+CGG - chr4.4:9176846-9176865 None:intergenic 35.0%
ATATGGGAGACCATTTAATG+AGG + chr4.4:9176313-9176332 MS.gene28393:CDS 35.0%
ATTGGGTTATGCATGAGTAT+AGG + chr4.4:9175735-9175754 MS.gene28393:CDS 35.0%
CAACAGAATAATCAAACGGT+GGG + chr4.4:9175656-9175675 MS.gene28393:CDS 35.0%
CCAACAAATTCATGTCAAAC+AGG + chr4.4:9176509-9176528 MS.gene28393:CDS 35.0%
CCTGTTAATTCTTGTCAAAC+AGG + chr4.4:9176593-9176612 MS.gene28393:CDS 35.0%
CCTGTTTGACAAGAATTAAC+AGG - chr4.4:9176596-9176615 None:intergenic 35.0%
CCTGTTTGACATGAATTTGT+TGG - chr4.4:9176512-9176531 None:intergenic 35.0%
CTACTTTAAATGCTAGGGTT+AGG + chr4.4:9175873-9175892 MS.gene28393:intron 35.0%
CTGGATATGAATCATTTGCA+GGG - chr4.4:9176850-9176869 None:intergenic 35.0%
CTGTTTGACAAGAATTCACA+GGG - chr4.4:9176724-9176743 None:intergenic 35.0%
CTGTTTGACATGAATTTGCA+GGG - chr4.4:9176766-9176785 None:intergenic 35.0%
GTATTTCAAAAAGAAGGTCC+TGG + chr4.4:9176272-9176291 MS.gene28393:CDS 35.0%
GTTAATTCTTGTCAAACAGG+AGG + chr4.4:9176596-9176615 MS.gene28393:CDS 35.0%
TCAACAGAATAATCAAACGG+TGG + chr4.4:9175655-9175674 MS.gene28393:CDS 35.0%
TCTGGATATGAATCATTTGC+AGG - chr4.4:9176851-9176870 None:intergenic 35.0%
TCTGTTTGACATGAATTTGC+AGG - chr4.4:9176767-9176786 None:intergenic 35.0%
TGAAAAGAATGTAAAGGGTC+AGG - chr4.4:9176677-9176696 None:intergenic 35.0%
TGAGTATAGGCTTGAAGATA+AGG + chr4.4:9175748-9175767 MS.gene28393:CDS 35.0%
TTATTTCGACTCCAACTTTC+AGG - chr4.4:9177501-9177520 None:intergenic 35.0%
! CTGTTTGACATGAATTTGCT+GGG - chr4.4:9176640-9176659 None:intergenic 35.0%
! TGAAGTAGGAATACCTTTTG+CGG + chr4.4:9176352-9176371 MS.gene28393:CDS 35.0%
! TTGACATTGTTATTGGTGTC+AGG + chr4.4:9177272-9177291 MS.gene28393:intron 35.0%
!! CATTGGTTGTTTCTAATCAC+TGG + chr4.4:9176007-9176026 MS.gene28393:intron 35.0%
!! TTCATGATAGTTTTGCATGC+AGG + chr4.4:9175955-9175974 MS.gene28393:intron 35.0%
!!! AACTGACTTTTTTGATGGCT+TGG + chr4.4:9177320-9177339 MS.gene28393:CDS 35.0%
AATGCAGACTTTAGCACACA+CGG + chr4.4:9177384-9177403 MS.gene28393:CDS 40.0%
ACAAGTTCTCTTGGTATGAC+AGG + chr4.4:9176229-9176248 MS.gene28393:intron 40.0%
ATCTAGACTCTGATACGTTC+TGG + chr4.4:9177466-9177485 MS.gene28393:CDS 40.0%
ATGAAACGAGCTACGGAAAT+TGG + chr4.4:9175599-9175618 MS.gene28393:CDS 40.0%
ATTAGATGTCACAGGCAAAC+TGG - chr4.4:9176396-9176415 None:intergenic 40.0%
CCAGCAAATTCATGTCAAAC+AGG + chr4.4:9176638-9176657 MS.gene28393:CDS 40.0%
CCTGTGAATTCTTGTCAAAC+AGG + chr4.4:9176722-9176741 MS.gene28393:CDS 40.0%
CCTGTTTGACAAGAATTCAC+AGG - chr4.4:9176725-9176744 None:intergenic 40.0%
CCTGTTTGACATGAATTTGC+TGG - chr4.4:9176641-9176660 None:intergenic 40.0%
CTATTTGACATGAACTCGCA+AGG - chr4.4:9176808-9176827 None:intergenic 40.0%
CTCCAGTCTTCCTCATTAAA+TGG - chr4.4:9176326-9176345 None:intergenic 40.0%
CTGTTTGACATGGATTTGCA+GGG - chr4.4:9176553-9176572 None:intergenic 40.0%
CTTGCGAGTTCATGTCAAAT+AGG + chr4.4:9176806-9176825 MS.gene28393:CDS 40.0%
GAATTAACAGGGTCTGGATA+AGG - chr4.4:9176584-9176603 None:intergenic 40.0%
GACCATTTAATGAGGAAGAC+TGG + chr4.4:9176321-9176340 MS.gene28393:CDS 40.0%
GGATTCCTACGTAATCTGTA+AGG + chr4.4:9176250-9176269 MS.gene28393:intron 40.0%
GGCATAAATCCTGTTTGACA+TGG - chr4.4:9176563-9176582 None:intergenic 40.0%
TAGGCTTGAAGATAAGGACT+TGG + chr4.4:9175754-9175773 MS.gene28393:CDS 40.0%
TATGAATCATTTGCAGGGTC+AGG - chr4.4:9176845-9176864 None:intergenic 40.0%
TATGAATCATTTGCAGGGTC+GGG - chr4.4:9176845-9176864 None:intergenic 40.0%
TATTCGCAACGAGAATAGGA+TGG - chr4.4:9176201-9176220 None:intergenic 40.0%
TGAAACGAGCTACGGAAATT+GGG + chr4.4:9175600-9175619 MS.gene28393:CDS 40.0%
TGACAAGAATTAACAGGGTC+TGG - chr4.4:9176590-9176609 None:intergenic 40.0%
TGTCAGGAAAACATTGCTGA+AGG + chr4.4:9177288-9177307 MS.gene28393:intron 40.0%
TTGACATGAATTTGCAGGGT+CGG - chr4.4:9176762-9176781 None:intergenic 40.0%
! AGGAATACCTTTTGCGGAAT+CGG + chr4.4:9176358-9176377 MS.gene28393:CDS 40.0%
!! TGACATGAATTTGTTGGGTC+CGG - chr4.4:9176506-9176525 None:intergenic 40.0%
!! TTGACATGAATTTGCTGGGT+CGG - chr4.4:9176636-9176655 None:intergenic 40.0%
!!! ATTGGGTTTTGGAAAGCTAC+TGG + chr4.4:9175617-9175636 MS.gene28393:CDS 40.0%
AAAAAGAAGGTCCTGGTCCT+AGG + chr4.4:9176279-9176298 MS.gene28393:CDS 45.0%
AGGAGACAATGCAAACTGCA+GGG - chr4.4:9177179-9177198 None:intergenic 45.0%
ATCCTTCGAAGGGTGAAATG+TGG + chr4.4:9175899-9175918 MS.gene28393:intron 45.0%
CAATTCTGATTGACACGGCA+TGG - chr4.4:9176474-9176493 None:intergenic 45.0%
CATGCCGTGTCAATCAGAAT+TGG + chr4.4:9176472-9176491 MS.gene28393:CDS 45.0%
CCTGCAAATCCATGTCAAAC+AGG + chr4.4:9176551-9176570 MS.gene28393:CDS 45.0%
CCTGTTTGACATGGATTTGC+AGG - chr4.4:9176554-9176573 None:intergenic 45.0%
CTAGGAATGGTGCGCAATAT+GGG + chr4.4:9176297-9176316 MS.gene28393:CDS 45.0%
GAATGTAAAGGGTCAGGAGA+AGG - chr4.4:9176671-9176690 None:intergenic 45.0%
GAATTCACAGGGTCTGGATA+AGG - chr4.4:9176713-9176732 None:intergenic 45.0%
GATAAGGACTTGGCTGACAA+AGG + chr4.4:9175764-9175783 MS.gene28393:CDS 45.0%
GCATGACGACTTCGATTTCA+TGG + chr4.4:9177434-9177453 MS.gene28393:CDS 45.0%
GGCACCAATTCTGATTGACA+CGG - chr4.4:9176479-9176498 None:intergenic 45.0%
GGCTGACAAAGGAATTGTTC+AGG + chr4.4:9175775-9175794 MS.gene28393:CDS 45.0%
GGTTAGGGTTATCCTTCGAA+GGG + chr4.4:9175889-9175908 MS.gene28393:intron 45.0%
GTGTGCTAAAGTCTGCATTC+TGG - chr4.4:9177383-9177402 None:intergenic 45.0%
TGACAAGAATTCACAGGGTC+TGG - chr4.4:9176719-9176738 None:intergenic 45.0%
! ATCAGAATTGGTGCCTTCTC+CGG + chr4.4:9176484-9176503 MS.gene28393:CDS 45.0%
! GACTGGAGTGATGATGAAGT+AGG + chr4.4:9176338-9176357 MS.gene28393:CDS 45.0%
! GAGCTACGGAAATTGGGTTT+TGG + chr4.4:9175606-9175625 MS.gene28393:CDS 45.0%
! TTCGACTCCAACTTTCAGGT+TGG - chr4.4:9177497-9177516 None:intergenic 45.0%
!! TCGACTCCAACTTTCAGGTT+GGG - chr4.4:9177496-9177515 None:intergenic 45.0%
!! TGACATGAATTTGCTGGGTC+GGG - chr4.4:9176635-9176654 None:intergenic 45.0%
!! TTTTGATGGCTTGGAAGACG+TGG + chr4.4:9177329-9177348 MS.gene28393:CDS 45.0%
!!! GTTTTGGAAAGCTACTGGTC+AGG + chr4.4:9175622-9175641 MS.gene28393:CDS 45.0%
ACAATGCAAACTGCAGGGGA+TGG - chr4.4:9177174-9177193 None:intergenic 50.0%
ACATGAACTCGCAAGGTCAG+GGG - chr4.4:9176801-9176820 None:intergenic 50.0%
AGACAACGGTGTATTGCCTG+AGG + chr4.4:9177039-9177058 MS.gene28393:CDS 50.0%
AGACTTTAGCACACACGGAC+TGG + chr4.4:9177389-9177408 MS.gene28393:CDS 50.0%
CACACTGGAAAATCACCTCG+TGG + chr4.4:9175701-9175720 MS.gene28393:CDS 50.0%
CCATATTGCGCACCATTCCT+AGG - chr4.4:9176299-9176318 None:intergenic 50.0%
CCTAGGAATGGTGCGCAATA+TGG + chr4.4:9176296-9176315 MS.gene28393:CDS 50.0%
CTCCACATTTCACCCTTCGA+AGG - chr4.4:9175904-9175923 None:intergenic 50.0%
CTTCTACGGGCAATGTTGTG+GGG + chr4.4:9177412-9177431 MS.gene28393:CDS 50.0%
GACATGAACTCGCAAGGTCA+GGG - chr4.4:9176802-9176821 None:intergenic 50.0%
GAGGAGACAATGCAAACTGC+AGG - chr4.4:9177180-9177199 None:intergenic 50.0%
GCTTCTACGGGCAATGTTGT+GGG + chr4.4:9177411-9177430 MS.gene28393:CDS 50.0%
GGAGACAATGCAAACTGCAG+GGG - chr4.4:9177178-9177197 None:intergenic 50.0%
GGGTTAGGGTTATCCTTCGA+AGG + chr4.4:9175888-9175907 MS.gene28393:intron 50.0%
TCATTTGCAGGGTCAGGCAA+AGG - chr4.4:9176839-9176858 None:intergenic 50.0%
TCGTGGTGATCGAACCGATT+GGG + chr4.4:9175718-9175737 MS.gene28393:CDS 50.0%
TGACATGAACTCGCAAGGTC+AGG - chr4.4:9176803-9176822 None:intergenic 50.0%
TGACATGGATTTGCAGGGTC+AGG - chr4.4:9176548-9176567 None:intergenic 50.0%
! CTTGGAAGACGTGGATTGCT+CGG + chr4.4:9177338-9177357 MS.gene28393:CDS 50.0%
! GTGGATTGCTCGGCTGAATT+GGG + chr4.4:9177348-9177367 MS.gene28393:CDS 50.0%
!! GAATTTGTTGGGTCCGGAGA+AGG - chr4.4:9176500-9176519 None:intergenic 50.0%
CTCGTGGTGATCGAACCGAT+TGG + chr4.4:9175717-9175736 MS.gene28393:CDS 55.0%
GAAGGTCCTGGTCCTAGGAA+TGG + chr4.4:9176284-9176303 MS.gene28393:CDS 55.0%
GAATTTGCAGGGTCGGAAGC+AGG - chr4.4:9176755-9176774 None:intergenic 55.0%
GGATTTGCAGGGTCAGGAGA+AGG - chr4.4:9176542-9176561 None:intergenic 55.0%
GGCAGACCCAACCTGAAAGT+TGG + chr4.4:9177487-9177506 MS.gene28393:CDS 55.0%
GGCTTCTACGGGCAATGTTG+TGG + chr4.4:9177410-9177429 MS.gene28393:CDS 55.0%
TCGGCTGAATTGGGATCTGC+TGG + chr4.4:9177357-9177376 MS.gene28393:CDS 55.0%
! AGTGCAGCCGATTCCGCAAA+AGG - chr4.4:9176368-9176387 None:intergenic 55.0%
! CGTGGATTGCTCGGCTGAAT+TGG + chr4.4:9177347-9177366 MS.gene28393:CDS 55.0%
!! ACACACGGACTGGCTTCTAC+GGG + chr4.4:9177399-9177418 MS.gene28393:CDS 55.0%
!! CAATCGGTTCGATCACCACG+AGG - chr4.4:9175719-9175738 None:intergenic 55.0%
!! CACACACGGACTGGCTTCTA+CGG + chr4.4:9177398-9177417 MS.gene28393:CDS 55.0%
!! GAATTTGCTGGGTCGGGAGA+CGG - chr4.4:9176629-9176648 None:intergenic 55.0%
GAACTCGCAAGGTCAGGGGA+CGG - chr4.4:9176797-9176816 None:intergenic 60.0%
TGCGCACCATTCCTAGGACC+AGG - chr4.4:9176293-9176312 None:intergenic 60.0%
TCATTTGCAGGGTCGGGCGC+AGG - chr4.4:9176950-9176969 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 9175599 9177524 9175599 ID=MS.gene28393
chr4.4 mRNA 9175599 9177524 9175599 ID=MS.gene28393.t1;Parent=MS.gene28393
chr4.4 exon 9175599 9175796 9175599 ID=MS.gene28393.t1.exon1;Parent=MS.gene28393.t1
chr4.4 CDS 9175599 9175796 9175599 ID=cds.MS.gene28393.t1;Parent=MS.gene28393.t1
chr4.4 exon 9176251 9177060 9176251 ID=MS.gene28393.t1.exon2;Parent=MS.gene28393.t1
chr4.4 CDS 9176251 9177060 9176251 ID=cds.MS.gene28393.t1;Parent=MS.gene28393.t1
chr4.4 exon 9177294 9177524 9177294 ID=MS.gene28393.t1.exon3;Parent=MS.gene28393.t1
chr4.4 CDS 9177294 9177524 9177294 ID=cds.MS.gene28393.t1;Parent=MS.gene28393.t1
Gene Sequence

>MS.gene28393

ATGAAACGAGCTACGGAAATTGGGTTTTGGAAAGCTACTGGTCAGGATCGAGCTGTTCAACAGAATAATCAAACGGTGGGGATGATTAAAACCCTAATATTTCACACTGGAAAATCACCTCGTGGTGATCGAACCGATTGGGTTATGCATGAGTATAGGCTTGAAGATAAGGACTTGGCTGACAAAGGAATTGTTCAGGTAGTCATTTCTTTCTTAGTTGTTTTATATGAGCTTTATATGGAGATATATGTGTATTGCTTGCCACATATTACAACTACTTTAAATGCTAGGGTTAGGGTTATCCTTCGAAGGGTGAAATGTGGAGTTCTAACTTCTAATAATTGATTACAGTGTAGTTCATGATAGTTTTGCATGCAGGATAATCCTTGAAATAGAATTATGTTATTACATTGGTTGTTTCTAATCACTGGTTTTACATTACCTTATAAAGTGCATTTTTTACTTTCGAATAGTTGAATTCTTGAAATAGAATTATGTTATTGCATGTTTTGTTTGACTAACACTCAAAATGAGATTTGCTGTTTTAACACGACAAAGTTAGTATCCCTTGCTTTCTTTTAATTATTTGATAGTTTTTTCCATCCTATTCTCGTTGCGAATATGTTTTTAACAAGTTCTCTTGGTATGACAGGATTCCTACGTAATCTGTAAGGTATTTCAAAAAGAAGGTCCTGGTCCTAGGAATGGTGCGCAATATGGGAGACCATTTAATGAGGAAGACTGGAGTGATGATGAAGTAGGAATACCTTTTGCGGAATCGGCTGCACTAGTTGCCAGTTTGCCTGTGACATCTAATTGTTCTGTTCTAAATGATCAGAATTTTCAAACTAGTGGATATATTGGTTCAATATCCATGCCGTGTCAATCAGAATTGGTGCCTTCTCCGGACCCAACAAATTCATGTCAAACAGGATTGACACCTTCTCCTGACCCTGCAAATCCATGTCAAACAGGATTTATGCCTTATCCAGACCCTGTTAATTCTTGTCAAACAGGAGGATTTATGCCGTCTCCCGACCCAGCAAATTCATGTCAAACAGGATTGACACCTTCTCCTGACCCTTTACATTCTTTTCAAACAGGATTTATGCCTTATCCAGACCCTGTGAATTCTTGTCAAACAGGATTTATGCCTGCTTCCGACCCTGCAAATTCATGTCAAACAGAATTTATGCCGTCCCCTGACCTTGCGAGTTCATGTCAAATAGGATTGATGCCTTTGCCTGACCCTGCAAATGATTCATATCCAGACAATCAAGCTGTTAATAACGATGATGATGATGATATTCTAGCAATGCTTGATATTTTCAAAGACAACGGCGTATTGCCTGCGCCCGACCCTGCAAATGATTCATATCCAAACAATCAAACTGTTAATAATGATGATGATGATATTATAGCAATGCTTGATATTTTTGAAGACAACGGTGTATTGCCTGAGGTATGCTAATCTGTTTTTTCTTCCTTTTACTTGTGATTTTCTGGAAACAGTGTGATGTTTCTTTTTTATGTCTGGTTTTAGTGTTCTCTATATTTGTAGACTTGTTTTATCCATCCCCTGCAGTTTGCATTGTCTCCTCATACTGTTGATGCCATTTTACTAAATCATAGCGATGTATATAGTCCTTGATTTCTTGAAGGATAACTGAGTTTTGACATTGTTATTGGTGTCAGGAAAACATTGCTGAAGGTGCACTCTTAACTGACTTTTTTGATGGCTTGGAAGACGTGGATTGCTCGGCTGAATTGGGATCTGCTGGCCAGAATGCAGACTTTAGCACACACGGACTGGCTTCTACGGGCAATGTTGTGGGGCATGACGACTTCGATTTCATGGAGTTGATTGATCTAGACTCTGATACGTTCTGGCAGACCCAACCTGAAAGTTGGAGTCGAAATAAATAA
Protein sequence

>MS.gene28393.t1

MKRATEIGFWKATGQDRAVQQNNQTVGMIKTLIFHTGKSPRGDRTDWVMHEYRLEDKDLADKGIVQDSYVICKVFQKEGPGPRNGAQYGRPFNEEDWSDDEVGIPFAESAALVASLPVTSNCSVLNDQNFQTSGYIGSISMPCQSELVPSPDPTNSCQTGLTPSPDPANPCQTGFMPYPDPVNSCQTGGFMPSPDPANSCQTGLTPSPDPLHSFQTGFMPYPDPVNSCQTGFMPASDPANSCQTEFMPSPDLASSCQIGLMPLPDPANDSYPDNQAVNNDDDDDILAMLDIFKDNGVLPAPDPANDSYPNNQTVNNDDDDIIAMLDIFEDNGVLPEENIAEGALLTDFFDGLEDVDCSAELGSAGQNADFSTHGLASTGNVVGHDDFDFMELIDLDSDTFWQTQPESWSRNK