AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28410.t1 MTR_8g093070 95.858 507 21 0 1 507 1 507 0.0 1006
MS.gene28410.t1 MTR_8g092440 85.714 154 20 1 1 154 1 152 8.94e-87 264
MS.gene28410.t1 MTR_3g072710 38.095 399 194 12 136 496 117 500 4.64e-66 222
MS.gene28410.t1 MTR_5g058860 38.060 402 210 14 125 496 105 497 6.21e-65 219
MS.gene28410.t1 MTR_3g065980 33.162 389 213 15 136 495 169 539 1.74e-40 153
MS.gene28410.t1 MTR_7g027190 31.683 404 224 15 126 495 287 672 2.47e-39 152
MS.gene28410.t1 MTR_4g076020 31.081 370 215 11 144 499 89 432 4.29e-37 142
MS.gene28410.t1 MTR_4g102790 29.648 398 235 16 136 504 289 670 2.23e-36 143
MS.gene28410.t1 MTR_7g074650 27.882 373 228 14 144 497 443 793 9.00e-34 136
MS.gene28410.t1 MTR_4g077760 33.138 341 191 16 177 495 229 554 3.94e-31 127
MS.gene28410.t1 MTR_2g082090 28.125 384 238 16 136 495 209 578 4.60e-27 115
MS.gene28410.t1 MTR_1g096030 30.633 395 226 18 136 495 77 458 1.14e-26 112
MS.gene28410.t1 MTR_5g094450 25.783 415 267 15 111 495 128 531 1.44e-25 110
MS.gene28410.t1 MTR_8g442410 26.823 384 246 13 136 495 152 524 1.62e-25 110
MS.gene28410.t1 MTR_1g069725 25.916 382 240 13 135 496 105 463 1.62e-25 109
MS.gene28410.t1 MTR_2g034260 26.500 400 251 14 126 495 197 583 2.14e-25 110
MS.gene28410.t1 MTR_4g133660 28.142 366 221 16 150 496 201 543 3.18e-25 109
MS.gene28410.t1 MTR_0092s0100 26.542 373 253 9 133 495 369 730 3.32e-25 110
MS.gene28410.t1 MTR_3g056110 27.778 396 249 17 123 495 160 541 4.11e-25 108
MS.gene28410.t1 MTR_0092s0100 26.542 373 253 9 133 495 366 727 4.45e-25 109
MS.gene28410.t1 MTR_5g097480 28.431 408 248 20 114 495 154 543 4.99e-25 108
MS.gene28410.t1 MTR_5g015490 26.943 386 249 16 138 497 113 491 1.15e-24 107
MS.gene28410.t1 MTR_4g064150 25.862 406 248 17 127 498 349 735 2.38e-24 107
MS.gene28410.t1 MTR_2g034250 27.654 405 241 16 126 495 197 584 3.07e-24 106
MS.gene28410.t1 MTR_3g089055 27.177 379 242 13 139 495 203 569 5.55e-24 105
MS.gene28410.t1 MTR_3g089055 27.177 379 242 13 139 495 198 564 6.77e-24 105
MS.gene28410.t1 MTR_2g097473 24.737 380 244 16 144 498 294 656 2.07e-23 104
MS.gene28410.t1 MTR_1g029420 27.895 380 238 15 139 495 225 591 2.21e-23 103
MS.gene28410.t1 MTR_2g097467 24.868 378 246 18 144 498 294 656 4.45e-23 103
MS.gene28410.t1 MTR_4g064200 26.425 386 241 15 139 498 284 652 4.78e-23 103
MS.gene28410.t1 MTR_2g026250 26.462 359 218 16 168 495 252 595 5.66e-23 102
MS.gene28410.t1 MTR_2g034280 26.799 403 244 16 126 495 190 574 7.21e-23 102
MS.gene28410.t1 MTR_2g097410 24.623 398 261 16 127 498 359 743 8.72e-23 102
MS.gene28410.t1 MTR_4g097080 26.425 386 245 14 138 497 132 504 1.27e-22 101
MS.gene28410.t1 MTR_7g062120 25.061 411 262 16 115 498 351 742 3.49e-22 100
MS.gene28410.t1 MTR_2g097463 24.615 390 255 17 134 498 282 657 3.95e-22 100
MS.gene28410.t1 MTR_4g104020 27.366 391 236 16 136 495 146 519 1.92e-21 97.8
MS.gene28410.t1 MTR_2g089100 24.138 406 252 14 139 499 52 446 7.74e-20 92.4
MS.gene28410.t1 MTR_4g064120 24.685 397 246 17 136 498 251 628 1.16e-19 92.8
MS.gene28410.t1 MTR_2g097350 23.678 397 272 13 122 498 257 642 1.95e-18 89.0
MS.gene28410.t1 MTR_4g026485 26.702 382 240 16 133 495 263 623 2.17e-18 89.0
MS.gene28410.t1 MTR_4g064160 25.823 395 240 18 136 498 313 686 4.96e-18 87.8
MS.gene28410.t1 MTR_2g097310 24.227 388 261 15 132 498 265 640 5.74e-18 87.4
MS.gene28410.t1 MTR_4g064180 25.460 326 198 14 136 434 251 558 9.59e-18 86.7
MS.gene28410.t1 MTR_8g077940 27.005 374 247 13 136 495 179 540 1.89e-17 85.5
MS.gene28410.t1 MTR_2g097390 22.980 396 276 14 122 498 258 643 2.53e-17 85.5
MS.gene28410.t1 MTR_5g015950 22.877 424 253 12 139 498 35 448 3.41e-15 78.2
MS.gene28410.t1 MTR_3g053270 22.877 424 253 12 139 498 35 448 3.41e-15 78.2
MS.gene28410.t1 MTR_2g099110 23.299 485 281 21 53 497 19 452 8.86e-15 77.0
MS.gene28410.t1 MTR_5g009080 28.502 207 134 9 136 333 53 254 3.31e-14 75.1
MS.gene28410.t1 MTR_1g086970 24.318 403 254 17 126 495 93 477 2.30e-13 72.8
MS.gene28410.t1 MTR_4g095500 22.565 421 254 14 139 497 59 469 3.87e-13 72.0
MS.gene28410.t1 MTR_2g097380 23.248 314 197 10 203 498 276 563 4.51e-13 72.0
MS.gene28410.t1 MTR_4g076140 28.641 206 133 8 136 332 45 245 6.92e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28410.t1 AT4G08250 32.905 389 228 14 136 501 104 482 6.97e-48 172
MS.gene28410.t1 AT1G55580 36.392 316 157 13 209 495 144 444 6.01e-43 158
MS.gene28410.t1 AT5G41920 32.275 378 220 14 136 495 40 399 2.02e-40 150
MS.gene28410.t1 AT3G03450 29.973 377 235 13 136 495 180 544 2.63e-37 144
MS.gene28410.t1 AT1G66350 29.178 377 227 14 136 495 152 505 1.58e-35 139
MS.gene28410.t1 AT5G17490 28.475 446 277 15 72 495 90 515 3.21e-35 138
MS.gene28410.t1 AT2G01570 29.610 385 221 16 136 495 221 580 1.84e-33 134
MS.gene28410.t1 AT1G14920 28.497 386 224 16 136 495 169 528 5.33e-32 129
MS.gene28410.t1 AT3G54220 27.441 379 225 16 144 497 298 651 1.72e-31 129
MS.gene28410.t1 AT1G63100 25.436 401 253 14 129 496 242 629 2.13e-30 125
MS.gene28410.t1 AT1G63100 25.436 401 253 14 129 496 267 654 3.71e-30 125
MS.gene28410.t1 AT5G66770 30.117 342 203 13 177 495 255 583 5.43e-30 124
MS.gene28410.t1 AT5G59450 27.792 403 241 17 136 506 224 608 8.33e-30 123
MS.gene28410.t1 AT3G50650 29.661 354 211 14 167 495 201 541 6.49e-29 120
MS.gene28410.t1 AT3G46600 27.830 424 255 16 103 498 149 549 1.29e-27 116
MS.gene28410.t1 AT3G46600 28.792 389 231 15 138 498 3 373 1.66e-27 114
MS.gene28410.t1 AT3G46600 27.830 424 255 16 103 498 181 581 1.90e-27 116
MS.gene28410.t1 AT2G04890 28.713 404 243 19 119 495 27 412 1.91e-27 114
MS.gene28410.t1 AT4G17230 28.000 400 241 16 128 497 144 526 3.07e-27 115
MS.gene28410.t1 AT4G17230 28.000 400 241 16 128 497 144 526 3.07e-27 115
MS.gene28410.t1 AT5G48150 28.325 406 245 17 118 495 102 489 8.16e-27 114
MS.gene28410.t1 AT5G48150 28.325 406 245 17 118 495 102 489 8.16e-27 114
MS.gene28410.t1 AT5G48150 28.325 406 245 17 118 495 102 489 8.16e-27 114
MS.gene28410.t1 AT5G48150 28.325 406 245 17 118 495 102 489 8.16e-27 114
MS.gene28410.t1 AT1G50600 26.357 387 255 13 129 495 149 525 1.24e-26 113
MS.gene28410.t1 AT1G50600 26.098 387 256 14 129 495 220 596 2.03e-26 113
MS.gene28410.t1 AT1G50600 26.098 387 256 14 129 495 224 600 2.16e-26 113
MS.gene28410.t1 AT4G37650 27.296 392 247 14 139 498 146 531 1.87e-25 110
MS.gene28410.t1 AT1G07530 26.526 426 255 17 104 498 369 767 3.34e-25 110
MS.gene28410.t1 AT1G21450 26.897 435 272 17 87 495 178 592 4.67e-24 106
MS.gene28410.t1 AT2G29060 26.500 400 240 19 132 497 277 656 1.12e-22 102
MS.gene28410.t1 AT2G29060 26.750 400 239 19 132 497 314 693 1.14e-22 102
MS.gene28410.t1 AT2G37650 24.682 393 261 13 127 497 323 702 1.82e-21 98.6
MS.gene28410.t1 AT2G37650 24.682 393 261 13 127 497 335 714 1.95e-21 98.6
MS.gene28410.t1 AT4G00150 27.851 377 229 17 135 495 204 553 2.37e-20 94.7
MS.gene28410.t1 AT5G52510 27.619 315 188 11 212 495 334 639 3.47e-18 88.2
MS.gene28410.t1 AT1G07520 23.650 389 257 14 139 498 318 695 1.62e-17 86.3
MS.gene28410.t1 AT1G07520 23.650 389 257 14 139 498 319 696 1.74e-17 86.3
MS.gene28410.t1 AT1G07520 23.650 389 257 14 139 498 154 531 2.40e-17 85.5
MS.gene28410.t1 AT1G07520 23.650 389 257 14 139 498 154 531 2.40e-17 85.5
MS.gene28410.t1 AT4G36710 27.371 369 248 14 138 495 125 484 3.57e-17 84.7
MS.gene28410.t1 AT2G29065 24.768 323 201 12 203 498 323 630 5.86e-17 84.3
MS.gene28410.t1 AT1G50420 24.598 435 243 17 136 495 54 478 1.09e-16 83.2
MS.gene28410.t1 AT3G49950 23.846 390 256 12 139 497 30 409 1.06e-15 79.7
MS.gene28410.t1 AT3G60630 24.224 322 181 12 209 495 328 621 4.97e-13 72.0

Find 75 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATACCTCTTTCTCTATA+AGG 0.158083 4.4:-8914464 None:intergenic
ATTTCATCTAGGATTGTATT+TGG 0.213674 4.4:-8913358 None:intergenic
ATTTCGTCTAGGATTGTATT+TGG 0.226044 4.4:-8913418 None:intergenic
GGAAGTTCAAGAAGAGTTAA+TGG 0.258644 4.4:+8913612 MS.gene28410:CDS
GCTTGTTTGGTAAAGGGTAT+TGG 0.274576 4.4:+8914634 MS.gene28410:CDS
AAGCTATTGGTTAGCTTGTT+TGG 0.278423 4.4:+8914621 MS.gene28410:CDS
CTGTGATTGCTGTTACTCTA+AGG 0.284969 4.4:-8914050 None:intergenic
CTAAACAATGCATCGTCTTT+AGG 0.289811 4.4:+8913724 MS.gene28410:CDS
AACAAGCCTTGGTGATGATT+TGG 0.290948 4.4:-8914295 None:intergenic
CTTTGACTTCTGTTTCAATC+TGG 0.297827 4.4:+8914703 MS.gene28410:CDS
CTTGTTTGGTAAAGGGTATT+GGG 0.313644 4.4:+8914635 MS.gene28410:CDS
CCAGGGAAGAAGATTCTTAT+TGG 0.318155 4.4:+8913587 MS.gene28410:CDS
GCGAAAATGTGTTTGTTTCC+AGG 0.325069 4.4:+8913569 MS.gene28410:CDS
CAATACCTCTTTCTCTATAA+GGG 0.331359 4.4:-8914463 None:intergenic
TGTGGTGGAGTTACCGAAAA+AGG 0.334912 4.4:+8913544 MS.gene28410:CDS
TATTTCACAGGCTAAGCTAT+TGG 0.357597 4.4:+8914608 MS.gene28410:CDS
CTCATAATCCATCACATTCA+TGG 0.366173 4.4:-8913239 None:intergenic
TGGTTAGCTTGTTTGGTAAA+GGG 0.385802 4.4:+8914628 MS.gene28410:CDS
TAAGGGCTAACTCCATCTTA+TGG 0.393772 4.4:-8914446 None:intergenic
AAGGCGATAGTTTATTGAAC+AGG 0.393824 4.4:+8913749 MS.gene28410:CDS
CCTTATAGAGAAAGAGGTAT+TGG 0.394224 4.4:+8914464 MS.gene28410:CDS
GATCAAACAATATTTCATCT+AGG 0.398157 4.4:-8913369 None:intergenic
AATCCAATGGCCACCGTTGA+TGG 0.402840 4.4:+8913996 MS.gene28410:CDS
TTGGTTAGCTTGTTTGGTAA+AGG 0.407587 4.4:+8914627 MS.gene28410:CDS
ATCAATGAGAAACTCCTGTT+TGG 0.411022 4.4:-8914249 None:intergenic
ACTCAAATCCATGTCCTTTG+TGG 0.411956 4.4:+8914353 MS.gene28410:CDS
TTTGACTTCTGTTTCAATCT+GGG 0.412837 4.4:+8914704 MS.gene28410:CDS
TACATCAGTGGATGCCAAAC+AGG 0.416877 4.4:+8914235 MS.gene28410:CDS
AGCTTCGAAAATCGCTTGAT+TGG 0.420785 4.4:-8913923 None:intergenic
CTTATAGAGAAAGAGGTATT+GGG 0.435957 4.4:+8914465 MS.gene28410:CDS
GAGATAAGAGATGTTGTGAT+TGG 0.464477 4.4:-8913396 None:intergenic
CAACCATCAACGGTGGCCAT+TGG 0.475147 4.4:-8913999 None:intergenic
CCAATAAGAATCTTCTTCCC+TGG 0.475253 4.4:-8913587 None:intergenic
CTAAGCTACACTCATGCAGC+TGG 0.477200 4.4:-8913297 None:intergenic
TCCACTAAAACAACAAGCCT+TGG 0.477235 4.4:-8914306 None:intergenic
GAAGCCTCGGTTCAACAAAC+AGG 0.483354 4.4:+8914087 MS.gene28410:CDS
CGAAAATGTGTTTGTTTCCA+GGG 0.485498 4.4:+8913570 MS.gene28410:CDS
GCAATTTGAGAACTGTAAGT+TGG 0.486800 4.4:+8914659 MS.gene28410:CDS
GTTAGCCCTTATAGAGAAAG+AGG 0.487244 4.4:+8914458 MS.gene28410:CDS
AATGCATCGTCTTTAGGAAA+AGG 0.491810 4.4:+8913730 MS.gene28410:CDS
TCATGCAGCTGGTTCATGTA+AGG 0.497438 4.4:-8913286 None:intergenic
GATTTCAAAGAAATTGAACT+TGG 0.498258 4.4:+8914186 MS.gene28410:CDS
TTACTCATATTATAATCTGT+TGG 0.507657 4.4:-8914729 None:intergenic
ACATGTTCTGAATGGAACTT+AGG 0.509744 4.4:-8913820 None:intergenic
AAAAGAGGAGAGAATATTGT+GGG 0.510893 4.4:+8914527 MS.gene28410:CDS
ACTGTAAGTTGGCTTTGTGT+TGG 0.525441 4.4:+8914670 MS.gene28410:CDS
CACGTTCGATACAGTAATGA+TGG 0.531854 4.4:+8914152 MS.gene28410:CDS
GGAAGAAGATTCTTATTGGA+AGG 0.535054 4.4:+8913591 MS.gene28410:CDS
GTGTAGAAACATGTTCTGAA+TGG 0.541579 4.4:-8913828 None:intergenic
CTTGATCGAAGATTTCGTCT+AGG 0.546863 4.4:-8913429 None:intergenic
ATTATCTACCATGAATGTGA+TGG 0.551569 4.4:+8913231 None:intergenic
TGCAGCTGGTTCATGTAAGG+AGG 0.555508 4.4:-8913283 None:intergenic
GATTCATGATATTGAAGTCG+AGG 0.561299 4.4:-8913489 None:intergenic
CATGAATCATGAACAAGCAA+TGG 0.561832 4.4:+8913504 MS.gene28410:CDS
TCTGTGGAGTAGCCATAAGA+TGG 0.563300 4.4:+8914434 MS.gene28410:CDS
TTGAAGGGGTATAGACGTGT+TGG 0.563890 4.4:+8914570 MS.gene28410:CDS
CGATCAGCAAAGTGAAGCCT+CGG 0.566253 4.4:+8914074 MS.gene28410:CDS
GCAGCTGGTTCATGTAAGGA+GGG 0.582532 4.4:-8913282 None:intergenic
TGTGATTGCTGTTACTCTAA+GGG 0.585072 4.4:-8914049 None:intergenic
GAGTACATGCAATATTTCAC+AGG 0.587078 4.4:+8914596 MS.gene28410:CDS
CGGTTCAACAAACAGGCAGA+AGG 0.592036 4.4:+8914094 MS.gene28410:CDS
ATTACTGTATCGAACGTGAA+CGG 0.593883 4.4:-8914147 None:intergenic
TTGCTGCAGCAATTCTAGCA+TGG 0.607187 4.4:-8913458 None:intergenic
ATTGCAAAGTCAACCATCAA+CGG 0.607337 4.4:-8914009 None:intergenic
TCTGCCTGTTTGTTGAACCG+AGG 0.614543 4.4:-8914091 None:intergenic
CAGATTGAAACAGAAGTCAA+AGG 0.620209 4.4:-8914702 None:intergenic
ATATCATGGAGGGAATCCAA+TGG 0.669550 4.4:+8913983 MS.gene28410:CDS
CTAGAGAATGAATTGTGTGG+TGG 0.675354 4.4:+8913529 MS.gene28410:CDS
CAGCTGGTTCATGTAAGGAG+GGG 0.676430 4.4:-8913281 None:intergenic
ATTCACTTGTAAGTACTCTG+TGG 0.679195 4.4:+8914418 MS.gene28410:CDS
TCAATTGTATGATACATCAG+TGG 0.691913 4.4:+8914223 MS.gene28410:CDS
AGATTATAATATGAGTAACA+AGG 0.703553 4.4:+8914734 MS.gene28410:CDS
GCAAAGTCAACCATCAACGG+TGG 0.711871 4.4:-8914006 None:intergenic
GCGTAACCAAATCATCACCA+AGG 0.731037 4.4:+8914289 MS.gene28410:CDS
GCTCTAGAGAATGAATTGTG+TGG 0.769887 4.4:+8913526 MS.gene28410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTTTTATTCATTGA+AGG + chr4.4:8914554-8914573 MS.gene28410:CDS 15.0%
!!! AGATTTTTTTATTCATTGAA+GGG + chr4.4:8914555-8914574 MS.gene28410:CDS 15.0%
!! AGATTATAATATGAGTAACA+AGG + chr4.4:8914734-8914753 MS.gene28410:CDS 20.0%
!! TTACTCATATTATAATCTGT+TGG - chr4.4:8914732-8914751 None:intergenic 20.0%
!!! AAAATGAGCAAATTTTACAT+AGG - chr4.4:8913899-8913918 None:intergenic 20.0%
!!! AAATGAGCAAATTTTACATA+GGG - chr4.4:8913898-8913917 None:intergenic 20.0%
!!! GATTTTTTTATTCATTGAAG+GGG + chr4.4:8914556-8914575 MS.gene28410:CDS 20.0%
! GATCAAACAATATTTCATCT+AGG - chr4.4:8913372-8913391 None:intergenic 25.0%
! GATTTCAAAGAAATTGAACT+TGG + chr4.4:8914186-8914205 MS.gene28410:CDS 25.0%
!! ATTTCATCTAGGATTGTATT+TGG - chr4.4:8913361-8913380 None:intergenic 25.0%
!!! ATTGATTTTGATATCATGGA+GGG + chr4.4:8913973-8913992 MS.gene28410:CDS 25.0%
!!! TGTCATTGATTTTGATATCA+TGG + chr4.4:8913969-8913988 MS.gene28410:CDS 25.0%
AAAAAGAGGAGAGAATATTG+TGG + chr4.4:8914526-8914545 MS.gene28410:CDS 30.0%
AAAAGAGGAGAGAATATTGT+GGG + chr4.4:8914527-8914546 MS.gene28410:CDS 30.0%
CAATACCTCTTTCTCTATAA+GGG - chr4.4:8914466-8914485 None:intergenic 30.0%
TCAATTGTATGATACATCAG+TGG + chr4.4:8914223-8914242 MS.gene28410:CDS 30.0%
! ATTTCGTCTAGGATTGTATT+TGG - chr4.4:8913421-8913440 None:intergenic 30.0%
! TTAGTTGATCTTTTGCTGAT+AGG + chr4.4:8913646-8913665 MS.gene28410:CDS 30.0%
! TTTGACTTCTGTTTCAATCT+GGG + chr4.4:8914704-8914723 MS.gene28410:CDS 30.0%
!! CTTATAGAGAAAGAGGTATT+GGG + chr4.4:8914465-8914484 MS.gene28410:CDS 30.0%
!!! CATTGATTTTGATATCATGG+AGG + chr4.4:8913972-8913991 MS.gene28410:CDS 30.0%
AAGGCGATAGTTTATTGAAC+AGG + chr4.4:8913749-8913768 MS.gene28410:CDS 35.0%
ACAGTTTCCTTGTGAAAAAG+AGG + chr4.4:8914512-8914531 MS.gene28410:CDS 35.0%
ATCAATGAGAAACTCCTGTT+TGG - chr4.4:8914252-8914271 None:intergenic 35.0%
ATTACTGTATCGAACGTGAA+CGG - chr4.4:8914150-8914169 None:intergenic 35.0%
ATTCACTTGTAAGTACTCTG+TGG + chr4.4:8914418-8914437 MS.gene28410:CDS 35.0%
ATTGCAAAGTCAACCATCAA+CGG - chr4.4:8914012-8914031 None:intergenic 35.0%
CAGATTGAAACAGAAGTCAA+AGG - chr4.4:8914705-8914724 None:intergenic 35.0%
CATGAATCATGAACAAGCAA+TGG + chr4.4:8913504-8913523 MS.gene28410:CDS 35.0%
CCAATACCTCTTTCTCTATA+AGG - chr4.4:8914467-8914486 None:intergenic 35.0%
CGAAAATGTGTTTGTTTCCA+GGG + chr4.4:8913570-8913589 MS.gene28410:CDS 35.0%
CTAAACAATGCATCGTCTTT+AGG + chr4.4:8913724-8913743 MS.gene28410:CDS 35.0%
GAGATAAGAGATGTTGTGAT+TGG - chr4.4:8913399-8913418 None:intergenic 35.0%
GAGTACATGCAATATTTCAC+AGG + chr4.4:8914596-8914615 MS.gene28410:CDS 35.0%
GATTCATGATATTGAAGTCG+AGG - chr4.4:8913492-8913511 None:intergenic 35.0%
GCAATTTGAGAACTGTAAGT+TGG + chr4.4:8914659-8914678 MS.gene28410:CDS 35.0%
GGAAGAAGATTCTTATTGGA+AGG + chr4.4:8913591-8913610 MS.gene28410:CDS 35.0%
GGAAGTTCAAGAAGAGTTAA+TGG + chr4.4:8913612-8913631 MS.gene28410:CDS 35.0%
TATTTCACAGGCTAAGCTAT+TGG + chr4.4:8914608-8914627 MS.gene28410:CDS 35.0%
! AATGCATCGTCTTTAGGAAA+AGG + chr4.4:8913730-8913749 MS.gene28410:CDS 35.0%
! ACATGTTCTGAATGGAACTT+AGG - chr4.4:8913823-8913842 None:intergenic 35.0%
! CCTTATAGAGAAAGAGGTAT+TGG + chr4.4:8914464-8914483 MS.gene28410:CDS 35.0%
! CTTTGACTTCTGTTTCAATC+TGG + chr4.4:8914703-8914722 MS.gene28410:CDS 35.0%
! GTGTAGAAACATGTTCTGAA+TGG - chr4.4:8913831-8913850 None:intergenic 35.0%
! TGGAAACAAACACATTTTCG+CGG - chr4.4:8913570-8913589 None:intergenic 35.0%
! TGTGATTGCTGTTACTCTAA+GGG - chr4.4:8914052-8914071 None:intergenic 35.0%
! TTGTGTCTTTTCTTTACGCA+AGG + chr4.4:8913772-8913791 MS.gene28410:CDS 35.0%
!! AAGCTATTGGTTAGCTTGTT+TGG + chr4.4:8914621-8914640 MS.gene28410:CDS 35.0%
!! ATTCTCTCCTCTTTTTCACA+AGG - chr4.4:8914522-8914541 None:intergenic 35.0%
!! CTTGTTTGGTAAAGGGTATT+GGG + chr4.4:8914635-8914654 MS.gene28410:CDS 35.0%
!! TGGTTAGCTTGTTTGGTAAA+GGG + chr4.4:8914628-8914647 MS.gene28410:CDS 35.0%
!! TTGGTTAGCTTGTTTGGTAA+AGG + chr4.4:8914627-8914646 MS.gene28410:CDS 35.0%
ACTCAAATCCATGTCCTTTG+TGG + chr4.4:8914353-8914372 MS.gene28410:CDS 40.0%
AGCTTCGAAAATCGCTTGAT+TGG - chr4.4:8913926-8913945 None:intergenic 40.0%
ATATCATGGAGGGAATCCAA+TGG + chr4.4:8913983-8914002 MS.gene28410:CDS 40.0%
CACGTTCGATACAGTAATGA+TGG + chr4.4:8914152-8914171 MS.gene28410:CDS 40.0%
CCAATAAGAATCTTCTTCCC+TGG - chr4.4:8913590-8913609 None:intergenic 40.0%
CCAGGGAAGAAGATTCTTAT+TGG + chr4.4:8913587-8913606 MS.gene28410:CDS 40.0%
CTAGAGAATGAATTGTGTGG+TGG + chr4.4:8913529-8913548 MS.gene28410:CDS 40.0%
CTCACAGAAAAACTCCACAA+AGG - chr4.4:8914370-8914389 None:intergenic 40.0%
CTTGATCGAAGATTTCGTCT+AGG - chr4.4:8913432-8913451 None:intergenic 40.0%
GAAAAACTCCACAAAGGACA+TGG - chr4.4:8914364-8914383 None:intergenic 40.0%
GCGAAAATGTGTTTGTTTCC+AGG + chr4.4:8913569-8913588 MS.gene28410:CDS 40.0%
GTTAGCCCTTATAGAGAAAG+AGG + chr4.4:8914458-8914477 MS.gene28410:CDS 40.0%
TAAGGGCTAACTCCATCTTA+TGG - chr4.4:8914449-8914468 None:intergenic 40.0%
TCCACTAAAACAACAAGCCT+TGG - chr4.4:8914309-8914328 None:intergenic 40.0%
! AAGAGATCTTTTGCTTGGAG+TGG - chr4.4:8913334-8913353 None:intergenic 40.0%
! CTGTGATTGCTGTTACTCTA+AGG - chr4.4:8914053-8914072 None:intergenic 40.0%
! CTTTGTGGAGTTTTTCTGTG+AGG + chr4.4:8914368-8914387 MS.gene28410:CDS 40.0%
! GAGACAAGAGATCTTTTGCT+TGG - chr4.4:8913339-8913358 None:intergenic 40.0%
! GCTTGTTTGGTAAAGGGTAT+TGG + chr4.4:8914634-8914653 MS.gene28410:CDS 40.0%
!! AACAAGCCTTGGTGATGATT+TGG - chr4.4:8914298-8914317 None:intergenic 40.0%
!! ACTGTAAGTTGGCTTTGTGT+TGG + chr4.4:8914670-8914689 MS.gene28410:CDS 40.0%
!! GCTCTAGAGAATGAATTGTG+TGG + chr4.4:8913526-8913545 MS.gene28410:CDS 40.0%
!!! ACCAAGGCTTGTTGTTTTAG+TGG + chr4.4:8914305-8914324 MS.gene28410:CDS 40.0%
GCGTAACCAAATCATCACCA+AGG + chr4.4:8914289-8914308 MS.gene28410:CDS 45.0%
TACATCAGTGGATGCCAAAC+AGG + chr4.4:8914235-8914254 MS.gene28410:CDS 45.0%
TCTGTGGAGTAGCCATAAGA+TGG + chr4.4:8914434-8914453 MS.gene28410:CDS 45.0%
TTGCTGCAGCAATTCTAGCA+TGG - chr4.4:8913461-8913480 None:intergenic 45.0%
! AGATCTTTTGCTTGGAGTGG+TGG - chr4.4:8913331-8913350 None:intergenic 45.0%
! CACATTTTCGCGGCCTTTTT+CGG - chr4.4:8913560-8913579 None:intergenic 45.0%
! GCGATTTTCGAAGCTACATC+TGG + chr4.4:8913931-8913950 MS.gene28410:CDS 45.0%
! TCATGCAGCTGGTTCATGTA+AGG - chr4.4:8913289-8913308 None:intergenic 45.0%
! TGTGGTGGAGTTACCGAAAA+AGG + chr4.4:8913544-8913563 MS.gene28410:CDS 45.0%
! TTGAAGGGGTATAGACGTGT+TGG + chr4.4:8914570-8914589 MS.gene28410:CDS 45.0%
AATCCAATGGCCACCGTTGA+TGG + chr4.4:8913996-8914015 MS.gene28410:CDS 50.0%
CGATCAGCAAAGTGAAGCCT+CGG + chr4.4:8914074-8914093 MS.gene28410:CDS 50.0%
CGGTTCAACAAACAGGCAGA+AGG + chr4.4:8914094-8914113 MS.gene28410:CDS 50.0%
CTAAGCTACACTCATGCAGC+TGG - chr4.4:8913300-8913319 None:intergenic 50.0%
GAAGCCTCGGTTCAACAAAC+AGG + chr4.4:8914087-8914106 MS.gene28410:CDS 50.0%
GCAAAGTCAACCATCAACGG+TGG - chr4.4:8914009-8914028 None:intergenic 50.0%
! CAGCTGGTTCATGTAAGGAG+GGG - chr4.4:8913284-8913303 None:intergenic 50.0%
! GCAGCTGGTTCATGTAAGGA+GGG - chr4.4:8913285-8913304 None:intergenic 50.0%
! TCTGCCTGTTTGTTGAACCG+AGG - chr4.4:8914094-8914113 None:intergenic 50.0%
! TGCAGCTGGTTCATGTAAGG+AGG - chr4.4:8913286-8913305 None:intergenic 50.0%
CAACCATCAACGGTGGCCAT+TGG - chr4.4:8914002-8914021 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 8913241 8914764 8913241 ID=MS.gene28410
chr4.4 mRNA 8913241 8914764 8913241 ID=MS.gene28410.t1;Parent=MS.gene28410
chr4.4 exon 8913241 8914764 8913241 ID=MS.gene28410.t1.exon1;Parent=MS.gene28410.t1
chr4.4 CDS 8913241 8914764 8913241 ID=cds.MS.gene28410.t1;Parent=MS.gene28410.t1
Gene Sequence

>MS.gene28410

ATGAATGTGATGGATTATGAGCAATTTGATTACTCATTCACCCCTCCTTACATGAACCAGCTGCATGAGTGTAGCTTAGAAAATTTACCACCACTCCAAGCAAAAGATCTCTTGTCTCCAAATACAATCCTAGATGAAATATTGTTTGATCAAGACCAATCACAACATCTCTTATCTCCAAATACAATCCTAGACGAAATCTTCGATCAAGAAGAGTCCATGCTAGAATTGCTGCAGCAACATACAAACCTCGACTTCAATATCATGAATCATGAACAAGCAATGGCTCTAGAGAATGAATTGTGTGGTGGAGTTACCGAAAAAGGCCGCGAAAATGTGTTTGTTTCCAGGGAAGAAGATTCTTATTGGAAGGAAGTTCAAGAAGAGTTAATGGAAGAGACAAGTTTAGTTGATCTTTTGCTGATAGGAGCTGAAGCTGCTGAATCAAAAAACATGACTCTTGCTTCTGACATAATCGAAAAACTAAACAATGCATCGTCTTTAGGAAAAGGCGATAGTTTATTGAACAGGTTGTGTCTTTTCTTTACGCAAGGATTGTATTATAAAACTACAAATGCTCCTAAGTTCCATTCAGAACATGTTTCTACACAGACAAGCACATTCTGTGTGTTTCAGATACTTCAAGAACTCTCTCCCTATGTAAAATTTGCTCATTTTACTGCCAATCAAGCGATTTTCGAAGCTACATCTGGTGTCGAAGATGTTCATGTCATTGATTTTGATATCATGGAGGGAATCCAATGGCCACCGTTGATGGTTGACTTTGCAATGAGAAAGAAGACTACTTCCCTTAGAGTAACAGCAATCACAGTCGATCAGCAAAGTGAAGCCTCGGTTCAACAAACAGGCAGAAGGCTCAAAGAGTTCGCTGATTCAATCAATTTTCCGTTCACGTTCGATACAGTAATGATGGTGAGTGAAGAAGATTTCAAAGAAATTGAACTTGGTGAAACATTTATTGTCAATTGTATGATACATCAGTGGATGCCAAACAGGAGTTTCTCATTGATCAAAGCTTTTCTCGATCGCGTAACCAAATCATCACCAAGGCTTGTTGTTTTAGTGGAAGAAGAACTGTTTAATTTTTCTAGACTCAAATCCATGTCCTTTGTGGAGTTTTTCTGTGAGGCTTTGCATCACTATATTGCAGTTTCTGATTCACTTGTAAGTACTCTGTGGAGTAGCCATAAGATGGAGTTAGCCCTTATAGAGAAAGAGGTATTGGGAAATAGAATCTTAGACAGTGTAAGACAGTTTCCTTGTGAAAAAGAGGAGAGAATATTGTGGGAAGAAAGATTTTTTTATTCATTGAAGGGGTATAGACGTGTTGGTATGAGTACATGCAATATTTCACAGGCTAAGCTATTGGTTAGCTTGTTTGGTAAAGGGTATTGGGTGCAATTTGAGAACTGTAAGTTGGCTTTGTGTTGGAAGTCAAGACCTTTGACTTCTGTTTCAATCTGGGTACCAACAGATTATAATATGAGTAACAAGGTCAAATAG
Protein sequence

>MS.gene28410.t1

MNVMDYEQFDYSFTPPYMNQLHECSLENLPPLQAKDLLSPNTILDEILFDQDQSQHLLSPNTILDEIFDQEESMLELLQQHTNLDFNIMNHEQAMALENELCGGVTEKGRENVFVSREEDSYWKEVQEELMEETSLVDLLLIGAEAAESKNMTLASDIIEKLNNASSLGKGDSLLNRLCLFFTQGLYYKTTNAPKFHSEHVSTQTSTFCVFQILQELSPYVKFAHFTANQAIFEATSGVEDVHVIDFDIMEGIQWPPLMVDFAMRKKTTSLRVTAITVDQQSEASVQQTGRRLKEFADSINFPFTFDTVMMVSEEDFKEIELGETFIVNCMIHQWMPNRSFSLIKAFLDRVTKSSPRLVVLVEEELFNFSRLKSMSFVEFFCEALHHYIAVSDSLVSTLWSSHKMELALIEKEVLGNRILDSVRQFPCEKEERILWEERFFYSLKGYRRVGMSTCNISQAKLLVSLFGKGYWVQFENCKLALCWKSRPLTSVSIWVPTDYNMSNKVK