AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28412

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28412.t1 MTR_8g093040 71.753 308 45 6 1 300 1 274 1.72e-136 388
MS.gene28412.t1 MTR_6g045327 53.674 313 117 5 1 303 1 295 7.48e-102 304
MS.gene28412.t1 MTR_4g021760 48.780 328 135 8 1 313 1 310 5.12e-93 279
MS.gene28412.t1 MTR_4g021855 50.996 251 100 5 1 240 1 239 6.97e-74 230
MS.gene28412.t1 MTR_4g021790 44.983 289 127 6 1 278 1 268 1.46e-70 221
MS.gene28412.t1 MTR_1g032570 37.731 379 160 9 12 320 9 381 1.20e-69 228
MS.gene28412.t1 MTR_4g021845 50.901 222 93 4 1 211 1 217 4.57e-69 215
MS.gene28412.t1 MTR_8g105600 37.216 352 163 10 14 324 9 343 3.30e-54 186
MS.gene28412.t1 MTR_2g034960 42.500 240 104 2 13 218 28 267 1.23e-52 182
MS.gene28412.t1 MTR_5g055260 45.923 233 85 7 1 223 1 202 5.01e-51 170
MS.gene28412.t1 MTR_8g079940 38.911 257 118 3 14 234 25 278 1.62e-50 176
MS.gene28412.t1 MTR_4g121020 40.496 242 108 2 13 218 30 271 4.71e-50 177
MS.gene28412.t1 MTR_4g121020 40.496 242 108 2 13 218 30 271 4.90e-50 177
MS.gene28412.t1 MTR_3g086100 37.868 272 129 4 14 247 13 282 1.60e-49 174
MS.gene28412.t1 MTR_1g032570 34.906 318 131 10 73 320 1 312 3.69e-44 159
MS.gene28412.t1 MTR_4g131600 36.364 253 128 2 4 223 10 262 5.60e-44 159
MS.gene28412.t1 MTR_4g131580 34.221 263 131 3 14 234 17 279 4.32e-42 154
MS.gene28412.t1 MTR_3g102600 37.037 216 112 1 13 228 7 198 8.16e-42 145
MS.gene28412.t1 MTR_6g016850 47.134 157 70 4 9 153 4 159 1.46e-41 142
MS.gene28412.t1 MTR_4g131570 35.857 251 123 3 14 226 17 267 7.29e-41 150
MS.gene28412.t1 MTR_4g023980 50.735 136 56 2 61 185 36 171 1.15e-40 140
MS.gene28412.t1 MTR_3g102600 37.688 199 100 1 13 211 7 181 7.14e-40 139
MS.gene28412.t1 MTR_3g106220 35.484 248 118 3 13 218 7 254 8.47e-40 148
MS.gene28412.t1 MTR_1g013170 35.246 244 120 2 13 218 4 247 8.37e-39 143
MS.gene28412.t1 MTR_2g450070 31.939 263 132 3 6 221 10 272 7.63e-38 142
MS.gene28412.t1 MTR_2g450070 31.939 263 132 3 6 221 10 272 8.96e-38 142
MS.gene28412.t1 MTR_7g117705 33.462 260 134 4 13 233 4 263 4.13e-36 135
MS.gene28412.t1 MTR_3g102590 36.634 202 113 3 13 213 7 194 1.02e-34 125
MS.gene28412.t1 MTR_3g102590 36.816 201 112 3 13 212 7 193 1.30e-34 126
MS.gene28412.t1 MTR_1g013180 32.812 256 134 2 1 218 143 398 2.15e-34 133
MS.gene28412.t1 MTR_3g102600 36.416 173 86 1 56 228 1 149 1.38e-32 119
MS.gene28412.t1 MTR_3g086100 35.681 213 97 4 73 247 1 211 1.61e-32 126
MS.gene28412.t1 MTR_3g102600 37.179 156 74 1 56 211 1 132 6.10e-31 114
MS.gene28412.t1 MTR_7g026400 33.500 200 105 3 44 218 17 213 8.68e-31 122
MS.gene28412.t1 MTR_0450s0040 30.364 247 152 6 17 246 7 250 1.05e-29 116
MS.gene28412.t1 MTR_4g121020 39.506 162 61 3 93 218 7 167 6.17e-25 105
MS.gene28412.t1 MTR_8g032710 26.299 308 157 8 18 268 21 315 2.91e-21 94.4
MS.gene28412.t1 MTR_1g067110 32.520 123 78 2 18 136 85 206 1.32e-15 78.2
MS.gene28412.t1 MTR_1g067110 32.520 123 78 2 18 136 85 206 1.35e-15 78.2
MS.gene28412.t1 MTR_4g061360 34.959 123 75 2 18 136 89 210 6.11e-15 76.3
MS.gene28412.t1 MTR_8g024260 32.258 124 81 1 16 136 34 157 6.79e-15 75.9
MS.gene28412.t1 MTR_7g118260 30.769 130 87 1 15 141 11 140 7.33e-15 75.5
MS.gene28412.t1 MTR_7g118260 31.200 125 83 1 15 136 11 135 1.18e-14 75.1
MS.gene28412.t1 MTR_3g092780 31.148 122 81 1 18 136 56 177 1.31e-14 75.1
MS.gene28412.t1 MTR_3g092780 31.148 122 81 1 18 136 56 177 1.33e-14 75.1
MS.gene28412.t1 MTR_3g092780 31.148 122 81 1 18 136 56 177 1.61e-14 74.7
MS.gene28412.t1 MTR_3g092780 31.148 122 81 1 18 136 56 177 1.71e-14 74.7
MS.gene28412.t1 MTR_4g064730 55.172 58 26 0 161 218 130 187 9.89e-13 68.2
MS.gene28412.t1 MTR_3g037390 27.869 122 85 1 18 136 24 145 7.96e-12 66.2
MS.gene28412.t1 MTR_4g108880 28.689 122 84 1 18 136 33 154 8.41e-12 66.2
MS.gene28412.t1 MTR_5g014040 29.197 137 90 4 5 139 6 137 1.10e-11 65.9
MS.gene28412.t1 MTR_5g014040 29.197 137 90 4 5 139 6 137 1.11e-11 65.9
MS.gene28412.t1 MTR_5g041350 48.571 70 34 2 162 230 15 83 1.56e-11 65.1
MS.gene28412.t1 MTR_7g089010 51.724 58 28 0 161 218 90 147 1.81e-11 64.3
MS.gene28412.t1 MTR_4g098870 51.923 52 25 0 161 212 329 380 8.42e-11 63.2
MS.gene28412.t1 MTR_4g098870 51.923 52 25 0 161 212 309 360 8.49e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28412.t1 AT2G01760 37.548 261 118 2 13 228 6 266 2.75e-50 171
MS.gene28412.t1 AT2G01760 33.910 289 118 3 13 228 6 294 1.88e-44 157
MS.gene28412.t1 AT3G16857 35.206 267 117 2 8 218 27 293 5.55e-44 160
MS.gene28412.t1 AT3G16857 35.206 267 117 2 8 218 27 293 8.91e-44 159
MS.gene28412.t1 AT1G67710 38.492 252 116 5 15 227 8 259 7.81e-43 155
MS.gene28412.t1 AT2G25180 36.905 252 124 4 1 218 1 251 1.05e-41 153
MS.gene28412.t1 AT4G31920 38.272 243 121 5 1 218 1 239 1.06e-39 147
MS.gene28412.t1 AT2G01760 35.323 201 85 2 73 228 1 201 2.11e-32 123
MS.gene28412.t1 AT3G16857 33.632 223 92 2 52 218 1 223 2.70e-32 127
MS.gene28412.t1 AT5G58080 31.381 239 130 3 13 218 13 250 6.81e-32 125
MS.gene28412.t1 AT1G49190 29.688 256 137 5 5 218 21 275 7.59e-29 115
MS.gene28412.t1 AT1G67710 37.113 194 83 5 73 227 1 194 1.45e-27 112
MS.gene28412.t1 AT3G62670 31.381 239 125 6 19 224 42 274 9.08e-27 109
MS.gene28412.t1 AT5G49240 28.125 256 135 3 3 215 27 276 5.11e-26 105
MS.gene28412.t1 AT1G49190 35.878 131 84 0 6 136 22 152 8.12e-21 93.6
MS.gene28412.t1 AT1G67710 35.632 174 73 5 93 227 12 185 8.78e-19 87.0
MS.gene28412.t1 AT5G60100 34.426 122 77 1 18 136 64 185 8.24e-18 84.3
MS.gene28412.t1 AT5G60100 34.426 122 77 1 18 136 64 185 8.38e-18 84.3
MS.gene28412.t1 AT5G60100 34.426 122 77 1 18 136 64 185 8.38e-18 84.3
MS.gene28412.t1 AT5G60100 34.426 122 77 1 18 136 64 185 8.38e-18 84.3
MS.gene28412.t1 AT5G60100 34.426 122 77 1 18 136 64 185 1.01e-17 83.6
MS.gene28412.t1 AT5G60100 34.426 122 77 1 18 136 64 185 1.01e-17 83.6
MS.gene28412.t1 AT5G60100 34.426 122 77 1 18 136 64 185 1.01e-17 83.6
MS.gene28412.t1 AT2G46790 33.333 123 79 1 18 137 37 159 1.43e-16 80.5
MS.gene28412.t1 AT2G46790 33.333 123 79 1 18 137 37 159 1.62e-16 80.5
MS.gene28412.t1 AT5G24470 31.967 122 80 1 18 136 50 171 9.35e-16 78.2
MS.gene28412.t1 AT5G59570 54.237 59 27 0 160 218 139 197 1.71e-13 70.5
MS.gene28412.t1 AT5G59570 54.237 59 27 0 160 218 139 197 1.71e-13 70.5
MS.gene28412.t1 AT5G02810 32.520 123 78 2 18 136 78 199 2.05e-13 71.6
MS.gene28412.t1 AT5G61380 30.328 122 82 1 18 136 19 140 4.35e-13 70.5
MS.gene28412.t1 AT3G46640 55.172 58 26 0 161 218 143 200 4.47e-13 69.3
MS.gene28412.t1 AT3G46640 55.172 58 26 0 161 218 143 200 4.47e-13 69.3
MS.gene28412.t1 AT3G46640 55.172 58 26 0 161 218 143 200 4.63e-13 69.3
MS.gene28412.t1 AT5G07210 39.423 104 61 2 132 234 191 293 5.94e-12 67.0
MS.gene28412.t1 AT5G07210 39.423 104 61 2 132 234 199 301 6.04e-12 67.0
MS.gene28412.t1 AT5G44190 53.704 54 25 0 159 212 144 197 7.05e-12 66.2
MS.gene28412.t1 AT5G44190 53.704 54 25 0 159 212 144 197 7.48e-12 65.9
MS.gene28412.t1 AT2G40970 50.000 58 29 0 161 218 104 161 8.73e-12 64.7
MS.gene28412.t1 AT5G05090 51.724 58 28 0 161 218 80 137 9.46e-12 64.7
MS.gene28412.t1 AT3G10760 51.724 58 28 0 161 218 104 161 1.27e-11 65.1
MS.gene28412.t1 AT3G04030 52.727 55 25 1 162 215 45 99 7.79e-11 63.2
MS.gene28412.t1 AT3G04030 52.727 55 25 1 162 215 45 99 8.60e-11 62.8

Find 55 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTTGATTCTCGATTAA+AGG 0.164354 4.4:+8893837 None:intergenic
GGAATATGGTATATTCAATT+AGG 0.202842 4.4:-8893034 None:intergenic
AATTGCATCGACAATTTCTA+AGG 0.211162 4.4:-8893686 MS.gene28412:CDS
TGTTTCCATCAAAAGTTTAA+AGG 0.235719 4.4:+8894154 None:intergenic
CAAACGGGGCTTGCGAATAT+TGG 0.275266 4.4:-8893866 MS.gene28412:CDS
TCCAGTTACAACATGTTCTA+AGG 0.282036 4.4:-8894186 MS.gene28412:intron
ATCGCTTTATGAATTGCTTT+TGG 0.284723 4.4:+8893522 None:intergenic
TAAAGAATAGAAAGTCAAAA+AGG 0.285756 4.4:-8893317 MS.gene28412:CDS
ATTCTTTAGATGTGTCTCTT+TGG 0.286927 4.4:+8893332 None:intergenic
ACATTTCCACAACAGCATTA+AGG 0.328612 4.4:+8894359 None:intergenic
AGAAATTGTCGATGCAATTC+AGG 0.341097 4.4:+8893690 None:intergenic
GCACAACCTCCATCGGAATA+TGG 0.347834 4.4:-8893048 MS.gene28412:CDS
CTCTTAAATCCTCTGGGAAT+TGG 0.352622 4.4:+8894410 None:intergenic
GCAAGTTGCTAGCCATTTGC+AGG 0.355161 4.4:-8893475 MS.gene28412:intron
CTAAGGGTTGTGAATGAACT+TGG 0.358456 4.4:-8893669 MS.gene28412:CDS
CATAAAGCGATGAATGTTCC+TGG 0.373937 4.4:-8893510 MS.gene28412:CDS
ATATACGATTCTTCTTCGTT+AGG 0.374538 4.4:+8893721 None:intergenic
TATACGATTCTTCTTCGTTA+GGG 0.386785 4.4:+8893722 None:intergenic
GAATATGGTATATTCAATTA+GGG 0.387647 4.4:-8893033 None:intergenic
GAACTCAATTGTGTTTCGTT+AGG 0.423423 4.4:+8893258 None:intergenic
ACAAGAACTCTTAAATCCTC+TGG 0.429240 4.4:+8894403 None:intergenic
TCTATTCCATGCAATTATCA+AGG 0.446387 4.4:-8894337 MS.gene28412:intron
AGAGTTGTCGTTGCATTGTA+TGG 0.450449 4.4:+8893185 None:intergenic
TGACAGGAATATTGATTTGT+TGG 0.454324 4.4:+8894054 None:intergenic
TAAATCCTCTGGGAATTGGC+TGG 0.455635 4.4:+8894414 None:intergenic
TGATGAAAGCTGTTGCAAAC+GGG 0.461960 4.4:-8893881 MS.gene28412:CDS
ATAAACATAATCAAAGCAAA+AGG 0.469172 4.4:-8893773 MS.gene28412:CDS
AAATCATATGAATCCATGTC+TGG 0.476246 4.4:+8894092 None:intergenic
TAAACATAATCAAAGCAAAA+GGG 0.478480 4.4:-8893772 MS.gene28412:CDS
GTGATGAAAGCTGTTGCAAA+CGG 0.482441 4.4:-8893882 MS.gene28412:CDS
TATATATTCTTACTGATGAC+AGG 0.490720 4.4:+8894038 None:intergenic
GACAGGAATATTGATTTGTT+GGG 0.492437 4.4:+8894055 None:intergenic
CGAAGAAGAATCGTATATCA+TGG 0.494656 4.4:-8893716 MS.gene28412:CDS
TGATTTGTTGGGTAACATGT+CGG 0.535639 4.4:+8894066 None:intergenic
ATAGTGATGATGAATCATCT+TGG 0.538208 4.4:+8893076 None:intergenic
AGAGGATTTAAGAGTTCTTG+TGG 0.539349 4.4:-8894401 MS.gene28412:CDS
CAAGAACTCTTAAATCCTCT+GGG 0.542866 4.4:+8894404 None:intergenic
CATCTTGGATCATGTAGGAC+TGG 0.543187 4.4:+8893091 None:intergenic
AACAAACCTTGATAATTGCA+TGG 0.547307 4.4:+8894331 None:intergenic
TGAAGCTCAAATGCCAGACA+TGG 0.549847 4.4:-8894105 MS.gene28412:CDS
TGTGCAGTGATGTGTGATGA+TGG 0.552839 4.4:-8893918 MS.gene28412:intron
AAATCCTCTGGGAATTGGCT+GGG 0.555538 4.4:+8894415 None:intergenic
TGATGTCCTTAATGCTGTTG+TGG 0.557832 4.4:-8894365 MS.gene28412:CDS
ATACGAAGCACAACCTCCAT+CGG 0.580105 4.4:-8893055 MS.gene28412:CDS
ATACGATTCTTCTTCGTTAG+GGG 0.588904 4.4:+8893723 None:intergenic
AATATACCATATTCCGATGG+AGG 0.592529 4.4:+8893042 None:intergenic
ATTGCATCGACAATTTCTAA+GGG 0.597352 4.4:-8893685 MS.gene28412:CDS
AGAATCAAATCAAGAACATG+TGG 0.615053 4.4:-8893827 MS.gene28412:CDS
GATGAAAGCTGTTGCAAACG+GGG 0.621578 4.4:-8893880 MS.gene28412:CDS
AAACATAATCAAAGCAAAAG+GGG 0.630491 4.4:-8893771 MS.gene28412:CDS
TGAATCATCTTGGATCATGT+AGG 0.631847 4.4:+8893086 None:intergenic
TTGAATATACCATATTCCGA+TGG 0.640230 4.4:+8893039 None:intergenic
AACAAAGGGCTGTGTTGATG+TGG 0.643059 4.4:-8894135 MS.gene28412:CDS
ACATGTGGCAGTGTGTTGCA+AGG 0.672995 4.4:-8893812 MS.gene28412:CDS
ACTTCCCAGCCAATTCCCAG+AGG 0.715638 4.4:-8894419 MS.gene28412:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAGAAAATAAAAATTAT+TGG + chr4.4:8893859-8893878 None:intergenic 10.0%
!! AAAGAATAGAAAGTCAAAAA+GGG - chr4.4:8894164-8894183 MS.gene28412:CDS 20.0%
!! ATAAACATAATCAAAGCAAA+AGG - chr4.4:8893707-8893726 MS.gene28412:CDS 20.0%
!! TAAACATAATCAAAGCAAAA+GGG - chr4.4:8893708-8893727 MS.gene28412:CDS 20.0%
!! TAAAGAATAGAAAGTCAAAA+AGG - chr4.4:8894163-8894182 MS.gene28412:CDS 20.0%
!!! TGTTTTTTGTATTAATGTAG+TGG - chr4.4:8894030-8894049 MS.gene28412:intron 20.0%
! AAACATAATCAAAGCAAAAG+GGG - chr4.4:8893709-8893728 MS.gene28412:CDS 25.0%
! AAGAATAGAAAGTCAAAAAG+GGG - chr4.4:8894165-8894184 MS.gene28412:CDS 25.0%
! GAAAAAGAATCTTACTATCA+AGG + chr4.4:8893837-8893856 None:intergenic 25.0%
! TATATATTCTTACTGATGAC+AGG + chr4.4:8893445-8893464 None:intergenic 25.0%
! TGTTTCCATCAAAAGTTTAA+AGG + chr4.4:8893329-8893348 None:intergenic 25.0%
!! AAACTTTTGATGGAAACAAA+GGG - chr4.4:8893331-8893350 MS.gene28412:CDS 25.0%
!! CTGAGTTCATTAAATTTTCT+GGG + chr4.4:8894275-8894294 None:intergenic 25.0%
!! TAAACTTTTGATGGAAACAA+AGG - chr4.4:8893330-8893349 MS.gene28412:CDS 25.0%
!! TTGATTTGATTCTCGATTAA+AGG + chr4.4:8893646-8893665 None:intergenic 25.0%
!! TTTTGCCTTTAAACTTTTGA+TGG - chr4.4:8893321-8893340 MS.gene28412:CDS 25.0%
AAATCATATGAATCCATGTC+TGG + chr4.4:8893391-8893410 None:intergenic 30.0%
ACAAAAAACAAACCTGCAAA+TGG + chr4.4:8894020-8894039 None:intergenic 30.0%
AGAATAGAAAGTCAAAAAGG+GGG - chr4.4:8894166-8894185 MS.gene28412:CDS 30.0%
AGAATCAAATCAAGAACATG+TGG - chr4.4:8893653-8893672 MS.gene28412:intron 30.0%
ATATACGATTCTTCTTCGTT+AGG + chr4.4:8893762-8893781 None:intergenic 30.0%
ATTCTTTAGATGTGTCTCTT+TGG + chr4.4:8894151-8894170 None:intergenic 30.0%
CAAAAGTTCAAGAATTTCAC+AGG + chr4.4:8893228-8893247 None:intergenic 30.0%
TATACGATTCTTCTTCGTTA+GGG + chr4.4:8893761-8893780 None:intergenic 30.0%
TCTATTCCATGCAATTATCA+AGG - chr4.4:8893143-8893162 MS.gene28412:CDS 30.0%
TTGAATATACCATATTCCGA+TGG + chr4.4:8894444-8894463 None:intergenic 30.0%
! AACAAACCTTGATAATTGCA+TGG + chr4.4:8893152-8893171 None:intergenic 30.0%
! AATTGCATCGACAATTTCTA+AGG - chr4.4:8893794-8893813 MS.gene28412:CDS 30.0%
! ATAGTGATGATGAATCATCT+TGG + chr4.4:8894407-8894426 None:intergenic 30.0%
! ATCGCTTTATGAATTGCTTT+TGG + chr4.4:8893961-8893980 None:intergenic 30.0%
! ATTGCATCGACAATTTCTAA+GGG - chr4.4:8893795-8893814 MS.gene28412:CDS 30.0%
! CTGTGAAATTCTTGAACTTT+TGG - chr4.4:8893226-8893245 MS.gene28412:CDS 30.0%
! GCTGAGTTCATTAAATTTTC+TGG + chr4.4:8894276-8894295 None:intergenic 30.0%
!! GACAGGAATATTGATTTGTT+GGG + chr4.4:8893428-8893447 None:intergenic 30.0%
!! TGACAGGAATATTGATTTGT+TGG + chr4.4:8893429-8893448 None:intergenic 30.0%
AATATACCATATTCCGATGG+AGG + chr4.4:8894441-8894460 None:intergenic 35.0%
ACAAGAACTCTTAAATCCTC+TGG + chr4.4:8893080-8893099 None:intergenic 35.0%
ACATTTCCACAACAGCATTA+AGG + chr4.4:8893124-8893143 None:intergenic 35.0%
AGAAATTGTCGATGCAATTC+AGG + chr4.4:8893793-8893812 None:intergenic 35.0%
AGAGGATTTAAGAGTTCTTG+TGG - chr4.4:8893079-8893098 MS.gene28412:CDS 35.0%
ATACGATTCTTCTTCGTTAG+GGG + chr4.4:8893760-8893779 None:intergenic 35.0%
CAAGAACTCTTAAATCCTCT+GGG + chr4.4:8893079-8893098 None:intergenic 35.0%
CGAAGAAGAATCGTATATCA+TGG - chr4.4:8893764-8893783 MS.gene28412:CDS 35.0%
GAACTCAATTGTGTTTCGTT+AGG + chr4.4:8894225-8894244 None:intergenic 35.0%
GGAGAGACAAAAATTTACAC+AGG + chr4.4:8893918-8893937 None:intergenic 35.0%
TAGAACATGTTGTAACTGGA+AGG + chr4.4:8893294-8893313 None:intergenic 35.0%
TCCAGTTACAACATGTTCTA+AGG - chr4.4:8893294-8893313 MS.gene28412:CDS 35.0%
TGAATCATCTTGGATCATGT+AGG + chr4.4:8894397-8894416 None:intergenic 35.0%
! GCTTTGCCTCTGAAAAATAA+GGG + chr4.4:8893939-8893958 None:intergenic 35.0%
!! TGATTTGTTGGGTAACATGT+CGG + chr4.4:8893417-8893436 None:intergenic 35.0%
AGAGTTGTCGTTGCATTGTA+TGG + chr4.4:8894298-8894317 None:intergenic 40.0%
ATAGAAAGTCAAAAAGGGGG+CGG - chr4.4:8894169-8894188 MS.gene28412:CDS 40.0%
CATAAAGCGATGAATGTTCC+TGG - chr4.4:8893970-8893989 MS.gene28412:intron 40.0%
CTAAGGGTTGTGAATGAACT+TGG - chr4.4:8893811-8893830 MS.gene28412:CDS 40.0%
GCCTTAGAACATGTTGTAAC+TGG + chr4.4:8893298-8893317 None:intergenic 40.0%
GTGATGAAAGCTGTTGCAAA+CGG - chr4.4:8893598-8893617 MS.gene28412:intron 40.0%
TGATGAAAGCTGTTGCAAAC+GGG - chr4.4:8893599-8893618 MS.gene28412:intron 40.0%
TGATGTCCTTAATGCTGTTG+TGG - chr4.4:8893115-8893134 MS.gene28412:CDS 40.0%
! ACTTGCTCTTTTGTCAAACC+AGG + chr4.4:8893991-8894010 None:intergenic 40.0%
! CTCTTAAATCCTCTGGGAAT+TGG + chr4.4:8893073-8893092 None:intergenic 40.0%
! GGCTTTGCCTCTGAAAAATA+AGG + chr4.4:8893940-8893959 None:intergenic 40.0%
!! GGACTGGTCAGTAAAAATGT+TGG + chr4.4:8894376-8894395 None:intergenic 40.0%
!!! GTCTCTCCCTTATTTTTCAG+AGG - chr4.4:8893930-8893949 MS.gene28412:intron 40.0%
ATACGAAGCACAACCTCCAT+CGG - chr4.4:8894425-8894444 MS.gene28412:CDS 45.0%
CATCTTGGATCATGTAGGAC+TGG + chr4.4:8894392-8894411 None:intergenic 45.0%
GATGAAAGCTGTTGCAAACG+GGG - chr4.4:8893600-8893619 MS.gene28412:intron 45.0%
TGAAGCTCAAATGCCAGACA+TGG - chr4.4:8893375-8893394 MS.gene28412:intron 45.0%
TGTGCAGTGATGTGTGATGA+TGG - chr4.4:8893562-8893581 MS.gene28412:intron 45.0%
! AAATCCTCTGGGAATTGGCT+GGG + chr4.4:8893068-8893087 None:intergenic 45.0%
! AACAAAGGGCTGTGTTGATG+TGG - chr4.4:8893345-8893364 MS.gene28412:CDS 45.0%
! TAAATCCTCTGGGAATTGGC+TGG + chr4.4:8893069-8893088 None:intergenic 45.0%
ACATGTGGCAGTGTGTTGCA+AGG - chr4.4:8893668-8893687 MS.gene28412:CDS 50.0%
CAAACGGGGCTTGCGAATAT+TGG - chr4.4:8893614-8893633 MS.gene28412:intron 50.0%
GCAAGTTGCTAGCCATTTGC+AGG - chr4.4:8894005-8894024 MS.gene28412:intron 50.0%
GCACAACCTCCATCGGAATA+TGG - chr4.4:8894432-8894451 MS.gene28412:CDS 50.0%
ACTTCCCAGCCAATTCCCAG+AGG - chr4.4:8893061-8893080 MS.gene28412:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 8893035 8894467 8893035 ID=MS.gene28412
chr4.4 mRNA 8893035 8894467 8893035 ID=MS.gene28412.t1;Parent=MS.gene28412
chr4.4 exon 8894338 8894467 8894338 ID=MS.gene28412.t1.exon1;Parent=MS.gene28412.t1
chr4.4 CDS 8894338 8894467 8894338 ID=cds.MS.gene28412.t1;Parent=MS.gene28412.t1
chr4.4 exon 8894051 8894203 8894051 ID=MS.gene28412.t1.exon2;Parent=MS.gene28412.t1
chr4.4 CDS 8894051 8894203 8894051 ID=cds.MS.gene28412.t1;Parent=MS.gene28412.t1
chr4.4 exon 8893661 8893933 8893661 ID=MS.gene28412.t1.exon3;Parent=MS.gene28412.t1
chr4.4 CDS 8893661 8893933 8893661 ID=cds.MS.gene28412.t1;Parent=MS.gene28412.t1
chr4.4 exon 8893476 8893552 8893476 ID=MS.gene28412.t1.exon4;Parent=MS.gene28412.t1
chr4.4 CDS 8893476 8893552 8893476 ID=cds.MS.gene28412.t1;Parent=MS.gene28412.t1
chr4.4 exon 8893035 8893382 8893035 ID=MS.gene28412.t1.exon5;Parent=MS.gene28412.t1
chr4.4 CDS 8893035 8893382 8893035 ID=cds.MS.gene28412.t1;Parent=MS.gene28412.t1
Gene Sequence

>MS.gene28412

ATGGCTCTTTTTAACGATATAGAGTCACTTCCCAGCCAATTCCCAGAGGATTTAAGAGTTCTTGTGGTTGATCATGACACTGATGTCCTTAATGCTGTTGTGGAAATGTCTATTCCATGCAATTATCAAGGTTTGTTAATTAATTTATCATATATACTTTGTTCAATTGCAAATTAAATTATGTTATTAACCTGTGAAATTCTTGAACTTTTGGCATAATTTCTTACTTTTGTGTATTTTTATGTATTCTTTTTTTCCTTCCAGTTACAACATGTTCTAAGGCTTCTTTTGCCTTTAAACTTTTGATGGAAACAAAGGGCTGTGTTGATGTGGTACTAATTGAAGCTCAAATGCCAGACATGGATTCATATGATTTTGTCCGACATGTTACCCAACAAATCAATATTCCTGTCATCAGTAAGAATATATATAATTAACTATATTACAAAATTATATCACATAATTAATTTATTCATATACTTATGTATGACATTGTTATTTATTATTTAATTATAATGTTAATTACATGTGCAGTGATGTGTGATGATGGATCAACAAGTGCAGTGATGAAAGCTGTTGCAAACGGGGCTTGCGAATATTGGATTAAGCCTTTAATCGAGAATCAAATCAAGAACATGTGGCAGTGTGTTGCAAGGAAATTTTTCAATGAAAATAAACATAATCAAAGCAAAAGGGGAAGAGATGATAATGCTAATTATCAACCCCTAACGAAGAAGAATCGTATATCATGGACACCTGAATTGCATCGACAATTTCTAAGGGTTGTGAATGAACTTGGCCTTGATAGTAAGATTCTTTTTCCAATAATTTTTATTTTCTTTCTCTTATTTTCTTTGTTTGTATACTTCAAGAAACTAAACCTGTGTAAATTTTTGTCTCTCCCTTATTTTTCAGAGGCAAAGCCAAAAGCAATTCATAAAGCGATGAATGTTCCTGGTTTGACAAAAGAGCAAGTTGCTAGCCATTTGCAGGTTTGTTTTTTGTATTAATGTAGTGGTAGTGATTTTGTTTTGTGTGATTTTAAATTATAATTTATATTAACATTAAGATTTGAATATTGACAGAGAGTGATACTTGATTTGAAGAGTTCAACCAAAGAGACACATCTAAAGAATAGAAAGTCAAAAAGGGGGCGGAAAAAATCAAAGTTTGATCAATATGATGTTCCTAACGAAACACAATTGAGTTCTGAAGCTGTTCACTCAATACAACAAGACCCAGAAAATTTAATGAACTCAGCCATACAATGCAACGACAACTCTCATGCACAACAAGATCTGATGACATTCGACGATTATGAAGATTTTGACATTATTGCCAACATTTTTACTGACCAGTCCTACATGATCCAAGATGATTCATCATCACTATACGAAGCACAACCTCCATCGGAATATGGTATATTCAATTAG
Protein sequence

>MS.gene28412.t1

MALFNDIESLPSQFPEDLRVLVVDHDTDVLNAVVEMSIPCNYQVTTCSKASFAFKLLMETKGCVDVVLIEAQMPDMDSYDFVRHVTQQINIPVIMMCDDGSTSAVMKAVANGACEYWIKPLIENQIKNMWQCVARKFFNENKHNQSKRGRDDNANYQPLTKKNRISWTPELHRQFLRVVNELGLDKAKPKAIHKAMNVPGLTKEQVASHLQRVILDLKSSTKETHLKNRKSKRGRKKSKFDQYDVPNETQLSSEAVHSIQQDPENLMNSAIQCNDNSHAQQDLMTFDDYEDFDIIANIFTDQSYMIQDDSSSLYEAQPPSEYGIFN