AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28512

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28512.t1 MTR_8g078505 93.258 534 26 2 1 525 1 533 0.0 1019
MS.gene28512.t1 MTR_8g078490 67.550 302 83 3 239 525 947 1248 1.49e-130 410
MS.gene28512.t1 MTR_8g078490 50.000 216 79 5 51 240 374 586 8.46e-50 184
MS.gene28512.t1 MTR_5g018850 61.129 319 102 5 225 525 1188 1502 7.95e-117 377
MS.gene28512.t1 MTR_5g018850 43.448 145 75 1 64 201 492 636 1.92e-29 124
MS.gene28512.t1 MTR_4g132610 64.375 320 95 6 223 525 725 1042 6.29e-116 367
MS.gene28512.t1 MTR_4g132610 44.000 250 105 3 13 240 309 545 7.61e-45 170
MS.gene28512.t1 MTR_8g078700 58.468 248 59 4 6 240 20 236 2.07e-78 254
MS.gene28512.t1 MTR_4g132670 47.239 163 74 2 47 208 167 318 6.84e-34 132
MS.gene28512.t1 MTR_4g132670 51.685 89 39 1 54 138 24 112 6.26e-19 89.4
MS.gene28512.t1 MTR_5g013420 35.023 217 101 7 343 525 874 1084 3.89e-29 122
MS.gene28512.t1 MTR_5g013420 35.023 217 101 7 343 525 874 1084 3.95e-29 122
MS.gene28512.t1 MTR_1g069570 37.017 181 98 4 361 525 561 741 1.75e-27 117
MS.gene28512.t1 MTR_4g010830 34.884 215 104 7 343 525 765 975 1.85e-27 117
MS.gene28512.t1 MTR_1g080340 37.158 183 96 5 361 525 400 581 6.85e-27 115
MS.gene28512.t1 MTR_7g098390 37.569 181 97 4 361 525 517 697 2.58e-26 113
MS.gene28512.t1 MTR_7g088370 30.195 308 158 14 242 525 426 700 2.95e-25 110
MS.gene28512.t1 MTR_7g052480 33.790 219 101 6 346 525 338 551 1.65e-22 101
MS.gene28512.t1 MTR_3g011440 28.354 328 167 12 237 525 361 659 2.39e-22 101
MS.gene28512.t1 MTR_1g048950 29.851 335 180 12 204 525 236 528 7.54e-22 99.4
MS.gene28512.t1 MTR_8g042750 28.221 326 171 12 235 525 386 683 3.43e-21 97.8
MS.gene28512.t1 MTR_8g442490 35.758 165 56 4 68 226 1 121 4.65e-20 86.7
MS.gene28512.t1 MTR_1g069570 40.171 117 68 2 361 475 561 677 1.44e-19 92.8
MS.gene28512.t1 MTR_4g010550 32.178 202 111 6 343 525 265 459 7.09e-19 89.7
MS.gene28512.t1 MTR_7g084090 26.730 318 165 11 248 525 463 752 6.50e-18 87.8
MS.gene28512.t1 MTR_6g059310 32.386 176 96 4 359 525 85 246 8.33e-18 85.1
MS.gene28512.t1 MTR_6g059310 32.386 176 96 4 359 525 85 246 9.47e-18 85.1
MS.gene28512.t1 MTR_6g061270 30.189 212 109 5 351 525 480 689 1.57e-17 86.3
MS.gene28512.t1 MTR_8g070070 28.495 186 110 4 343 506 571 755 1.84e-17 86.3
MS.gene28512.t1 MTR_8g070075 26.882 186 113 4 343 506 564 748 9.85e-17 84.0
MS.gene28512.t1 MTR_7g450730 31.461 178 96 5 355 509 303 477 1.73e-16 82.4
MS.gene28512.t1 MTR_8g442480 51.282 78 37 1 95 171 13 90 2.54e-16 74.7
MS.gene28512.t1 MTR_7g445600 32.609 138 60 2 379 507 243 356 3.60e-16 80.5
MS.gene28512.t1 MTR_6g061200 29.032 248 135 9 298 525 434 660 4.06e-16 82.0
MS.gene28512.t1 MTR_8g071030 25.088 283 156 11 249 506 484 735 7.88e-16 81.3
MS.gene28512.t1 MTR_0071s0100 32.530 166 95 4 365 525 191 344 1.50e-15 79.0
MS.gene28512.t1 MTR_1g098000 32.099 162 96 3 369 525 78 230 2.02e-15 79.3
MS.gene28512.t1 MTR_3g088625 32.544 169 87 5 369 525 1307 1460 2.60e-15 80.1
MS.gene28512.t1 MTR_3g088625 32.544 169 87 5 369 525 1201 1354 2.60e-15 80.1
MS.gene28512.t1 MTR_1g098370 31.481 162 97 3 369 525 81 233 9.81e-15 77.0
MS.gene28512.t1 MTR_7g445530 30.851 188 88 6 362 507 326 513 4.15e-14 75.1
MS.gene28512.t1 MTR_6g043960 27.568 185 99 5 354 509 305 483 8.16e-14 74.3
MS.gene28512.t1 MTR_6g044690 27.568 185 99 5 354 509 387 565 1.15e-13 73.9
MS.gene28512.t1 MTR_6g027690 30.556 180 101 5 364 525 242 415 1.45e-13 73.2
MS.gene28512.t1 MTR_0376s0030 27.717 184 109 6 346 506 378 560 2.41e-13 72.8
MS.gene28512.t1 MTR_1g035420 27.322 183 110 5 346 506 463 644 4.12e-13 72.4
MS.gene28512.t1 MTR_1g064770 50.820 61 29 1 361 420 30 90 6.91e-13 65.1
MS.gene28512.t1 MTR_2g054460 27.027 185 100 5 354 509 287 465 1.18e-12 70.5
MS.gene28512.t1 MTR_1g035400 41.250 80 46 1 346 425 382 460 1.02e-11 67.8
MS.gene28512.t1 MTR_7g084090 28.070 171 98 4 248 417 463 609 5.44e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28512.t1 AT2G23740 54.392 296 110 6 248 525 1082 1370 1.88e-86 291
MS.gene28512.t1 AT2G23740 35.294 136 80 2 65 192 401 536 7.03e-18 88.2
MS.gene28512.t1 AT2G23740 54.392 296 110 6 248 525 1089 1377 2.40e-86 291
MS.gene28512.t1 AT2G23740 35.294 136 80 2 65 192 408 543 6.62e-18 88.2
MS.gene28512.t1 AT2G23740 54.392 296 110 6 248 525 1089 1377 2.40e-86 291
MS.gene28512.t1 AT2G23740 35.294 136 80 2 65 192 408 543 6.62e-18 88.2
MS.gene28512.t1 AT2G23740 54.392 296 110 6 248 525 1089 1377 2.40e-86 291
MS.gene28512.t1 AT2G23740 35.294 136 80 2 65 192 408 543 6.62e-18 88.2
MS.gene28512.t1 AT2G23740 58.547 234 84 4 248 475 1089 1315 3.90e-75 259
MS.gene28512.t1 AT2G23740 35.294 136 80 2 65 192 408 543 7.24e-18 87.8
MS.gene28512.t1 AT2G23740 58.547 234 84 4 248 475 1089 1315 3.90e-75 259
MS.gene28512.t1 AT2G23740 35.294 136 80 2 65 192 408 543 7.24e-18 87.8
MS.gene28512.t1 AT3G04380 33.921 227 114 9 316 525 223 430 1.78e-27 115
MS.gene28512.t1 AT3G04380 33.921 227 114 9 316 525 207 414 2.12e-27 115
MS.gene28512.t1 AT3G04380 33.921 227 114 9 316 525 223 430 2.23e-27 115
MS.gene28512.t1 AT3G04380 33.921 227 114 9 316 525 250 457 2.31e-27 115
MS.gene28512.t1 AT3G04380 33.921 227 114 9 316 525 250 457 2.34e-27 115
MS.gene28512.t1 AT3G04380 33.921 227 114 9 316 525 234 441 2.58e-27 115
MS.gene28512.t1 AT1G73100 28.923 325 164 13 237 525 371 664 4.74e-27 115
MS.gene28512.t1 AT1G04050 37.430 179 95 5 362 525 438 614 1.19e-26 114
MS.gene28512.t1 AT1G04050 37.430 179 95 5 362 525 542 718 1.21e-26 114
MS.gene28512.t1 AT1G04050 37.430 179 95 5 362 525 542 718 1.21e-26 114
MS.gene28512.t1 AT2G22740 31.944 216 110 6 343 525 574 785 1.14e-24 108
MS.gene28512.t1 AT2G22740 31.944 216 110 6 343 525 574 785 1.14e-24 108
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 457 632 3.29e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 457 632 3.29e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 457 632 3.29e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 457 632 3.29e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 549 724 3.40e-23 104
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 549 724 3.40e-23 104
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 506 681 3.58e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 506 681 3.58e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 506 681 3.58e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 506 681 3.58e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 529 704 3.64e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 529 704 3.64e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 526 701 3.73e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 526 701 3.73e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 526 701 3.73e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 526 701 3.73e-23 103
MS.gene28512.t1 AT5G43990 35.912 181 94 6 362 525 437 612 3.76e-23 103
MS.gene28512.t1 AT2G35160 33.155 187 96 5 355 525 580 753 1.49e-22 102
MS.gene28512.t1 AT2G35160 33.155 187 96 5 355 525 616 789 1.57e-22 102
MS.gene28512.t1 AT2G35160 33.155 187 96 5 355 525 616 789 1.57e-22 102
MS.gene28512.t1 AT2G35160 33.155 187 96 5 355 525 616 789 1.57e-22 102
MS.gene28512.t1 AT2G35160 33.155 187 96 5 355 525 616 789 1.57e-22 102
MS.gene28512.t1 AT5G13960 31.330 233 117 6 332 525 391 619 6.28e-21 97.1
MS.gene28512.t1 AT5G13960 31.602 231 119 6 332 525 257 485 6.47e-21 96.3
MS.gene28512.t1 AT3G03750 31.720 186 100 4 364 525 169 351 9.81e-19 88.2
MS.gene28512.t1 AT5G04940 29.954 217 113 8 343 525 454 665 8.50e-18 87.4
MS.gene28512.t1 AT5G04940 29.954 217 113 8 343 525 454 665 8.50e-18 87.4
MS.gene28512.t1 AT1G17770 26.829 328 178 14 233 525 388 688 1.07e-16 84.0
MS.gene28512.t1 AT2G33290 24.471 331 195 10 204 506 334 637 8.85e-15 77.8
MS.gene28512.t1 AT2G33290 24.471 331 195 10 204 506 334 637 8.85e-15 77.8
MS.gene28512.t1 AT2G33290 24.471 331 195 10 204 506 334 637 8.85e-15 77.8
MS.gene28512.t1 AT2G33290 24.471 331 195 10 204 506 334 637 8.85e-15 77.8
MS.gene28512.t1 AT4G30860 31.176 170 92 5 365 525 307 460 1.51e-14 76.6
MS.gene28512.t1 AT1G77300 30.636 173 85 6 369 525 1009 1162 1.94e-14 77.0
MS.gene28512.t1 AT1G77300 30.636 173 85 6 369 525 1009 1162 2.18e-14 77.0
MS.gene28512.t1 AT4G13460 39.286 84 50 1 343 426 451 533 1.60e-13 73.6
MS.gene28512.t1 AT4G13460 39.286 84 50 1 343 426 451 533 1.60e-13 73.6
MS.gene28512.t1 AT2G24740 29.843 191 92 8 371 525 566 750 1.54e-12 70.9
MS.gene28512.t1 AT1G76710 28.313 166 97 3 369 525 72 224 5.14e-12 68.6
MS.gene28512.t1 AT1G76710 28.313 166 97 3 369 525 72 224 5.15e-12 68.6
MS.gene28512.t1 AT1G76710 28.313 166 97 3 369 525 72 224 6.92e-12 68.2
MS.gene28512.t1 AT1G76710 28.313 166 97 3 369 525 72 224 6.92e-12 68.2
MS.gene28512.t1 AT3G59960 29.747 158 88 4 377 525 102 245 8.67e-12 66.6
MS.gene28512.t1 AT3G59960 29.747 158 88 4 377 525 102 245 8.67e-12 66.6
MS.gene28512.t1 AT3G59960 29.747 158 88 4 377 525 102 245 8.67e-12 66.6
MS.gene28512.t1 AT3G59960 29.747 158 88 4 377 525 102 245 1.00e-11 66.6
MS.gene28512.t1 AT2G44150 31.013 158 86 4 377 525 107 250 4.72e-11 65.1

Find 104 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAGAAAAGGACATATT+TGG 0.136959 8.3:+25666520 MS.gene28512:CDS
GGCGAAATGCTCAAGCTTTC+TGG 0.183364 8.3:+25665870 MS.gene28512:CDS
TTGATCTCCAATGGACTTTC+CGG 0.248032 8.3:-25665795 None:intergenic
CGAATATGATATATCGAAAA+TGG 0.248933 8.3:+25665275 MS.gene28512:CDS
ATGAAGAAAAGGACATATTT+GGG 0.300996 8.3:+25666521 MS.gene28512:CDS
GAACGGAGTTCGTGTTAAAT+TGG 0.304027 8.3:+25666924 MS.gene28512:CDS
AGTCCATGCAAATCAGGTTC+TGG 0.321251 8.3:-25665758 None:intergenic
CCGTTCTGCAATATTCTGTT+TGG 0.323633 8.3:-25666907 None:intergenic
GGTCTTTATGACAGAGAAAA+AGG 0.323703 8.3:+25666948 MS.gene28512:CDS
TATACGTGATCACACGTTTC+AGG 0.337540 8.3:-25666490 None:intergenic
TGTGGAGGTACAACTGGTTC+TGG 0.365849 8.3:+25665600 MS.gene28512:CDS
TGTAAATGGTCGAATCATAT+TGG 0.379997 8.3:+25666573 MS.gene28512:CDS
AGGTTCGCATACGATGTAAA+TGG 0.389210 8.3:+25666559 MS.gene28512:CDS
CCCATTTGTGGAGGTACAAC+TGG 0.389596 8.3:+25665594 MS.gene28512:CDS
TGTACGACGGTTTGAATTTG+AGG 0.401657 8.3:-25665700 None:intergenic
GGACTTGGTGTTGGCACATC+CGG 0.404270 8.3:+25665776 MS.gene28512:CDS
ATTCACAGGGATTCGGAGTA+AGG 0.405434 8.3:+25667043 MS.gene28512:intron
ACCAGTTGTACCTCCACAAA+TGG 0.405601 8.3:-25665595 None:intergenic
TATGAGCCTGTGCTCGTAGA+AGG 0.406674 8.3:+25667994 MS.gene28512:CDS
TTCTTTGCAAAGAACATCTC+AGG 0.407939 8.3:+25665849 MS.gene28512:CDS
TGATAATGAGAATGCTGTTA+AGG 0.413807 8.3:+25665920 MS.gene28512:CDS
TATGCGAACCTTCCGGCCAT+TGG 0.417185 8.3:-25666547 None:intergenic
TACATCGTATGCGAACCTTC+CGG 0.420636 8.3:-25666554 None:intergenic
ACACCAAGTCCATGCAAATC+AGG 0.422391 8.3:-25665764 None:intergenic
TGTCTAGGTTCATCAATAAC+AGG 0.425670 8.3:+25667357 MS.gene28512:CDS
TTATCTGATTCACAGGGATT+CGG 0.425686 8.3:+25667036 MS.gene28512:intron
TAGTAGAATCAATGACATAT+CGG 0.427363 8.3:-25667322 None:intergenic
GAAGGATATCCTTGTCTTTG+TGG 0.428480 8.3:+25668012 MS.gene28512:CDS
GCAAGCTAAGTGAATCCCTT+TGG 0.435509 8.3:-25665350 None:intergenic
ATCAAGGCCTGTGGATGAAA+TGG 0.435866 8.3:+25665413 MS.gene28512:CDS
CTGCAATATTCTGTTTGGAC+AGG 0.439001 8.3:-25666902 None:intergenic
GTAAACATCTCCCAAGGCTT+AGG 0.442228 8.3:-25666080 None:intergenic
GTCCATGCAAATCAGGTTCT+GGG 0.452226 8.3:-25665757 None:intergenic
GTCATTGATTCTACTACATA+TGG 0.457853 8.3:+25667329 MS.gene28512:CDS
CTTCGACCAATCTACTCATA+TGG 0.464079 8.3:-25667292 None:intergenic
GACGGTTTGAATTTGAGGTC+TGG 0.466441 8.3:-25665695 None:intergenic
TGAATAGCCATTTCATCCAC+AGG 0.471451 8.3:-25665420 None:intergenic
TTGATTCTGCCGCAAAGCTT+AGG 0.472145 8.3:-25665986 None:intergenic
AGTAGAATCAATGACATATC+GGG 0.477925 8.3:-25667321 None:intergenic
ATGCAATGACCTAAGCTTTG+CGG 0.481210 8.3:+25665977 MS.gene28512:CDS
GAACCTGATTTGCATGGACT+TGG 0.484117 8.3:+25665761 MS.gene28512:CDS
CATTGTCGATCCTTGGTACC+AGG 0.488120 8.3:-25665456 None:intergenic
CTACGCAGTTGATGAAGAAT+CGG 0.489211 8.3:-25666116 None:intergenic
ATGTATATCTTCCTAAGCCT+TGG 0.489667 8.3:+25666069 MS.gene28512:CDS
TGTATATCTTCCTAAGCCTT+GGG 0.489888 8.3:+25666070 MS.gene28512:CDS
GAGATGTTCTTTGCAAAGAA+AGG 0.490990 8.3:-25665846 None:intergenic
TGCTCGACATTGTCGATCCT+TGG 0.497731 8.3:-25665463 None:intergenic
CTACATATGGCAATGTGTCT+AGG 0.499868 8.3:+25667342 MS.gene28512:CDS
GATTGTAAGCATACACATAT+CGG 0.502921 8.3:+25667778 MS.gene28512:CDS
TGTAAGTATCGTTCCCGTCC+TGG 0.503749 8.3:+25665630 MS.gene28512:CDS
TGCTAACCATATGAGTAGAT+TGG 0.507042 8.3:+25667286 MS.gene28512:CDS
CATTGGCACAAAGCCGAGAC+GGG 0.509657 8.3:+25665672 MS.gene28512:CDS
GATTTGCATGGACTTGGTGT+TGG 0.512745 8.3:+25665767 MS.gene28512:CDS
AAAGTAAGCACTCCCATTTG+TGG 0.515955 8.3:+25665582 MS.gene28512:CDS
AAAATTAGGGTCGTCGCGAA+AGG 0.515962 8.3:+25665944 MS.gene28512:CDS
CGGAGTTCGTGTTAAATTGG+AGG 0.527617 8.3:+25666927 MS.gene28512:CDS
GTATATATTGCTACCAATTG+TGG 0.528485 8.3:-25665520 None:intergenic
CAAACCGTCGTACATTAAAG+AGG 0.536529 8.3:+25665709 MS.gene28512:CDS
CAGTGTGTGAGAACCACAAT+TGG 0.538082 8.3:+25665507 MS.gene28512:CDS
ATTTGAGGTCTGGCCCGTCT+CGG 0.538631 8.3:-25665685 None:intergenic
GGCACATCCGGAAAGTCCAT+TGG 0.540615 8.3:+25665788 MS.gene28512:CDS
ACATCATTGATGACCGACAC+TGG 0.541784 8.3:-25665819 None:intergenic
CAGGGATTCGGAGTAAGGGC+TGG 0.542170 8.3:+25667048 MS.gene28512:intron
CCAGTTGTACCTCCACAAAT+GGG 0.544157 8.3:-25665594 None:intergenic
GACACTGGATTGATCTCCAA+TGG 0.545742 8.3:-25665804 None:intergenic
CAGAGTTATTGTAGAGGGCA+CGG 0.549634 8.3:+25667756 MS.gene28512:intron
ATTTGTAGAACCTGGTACCA+AGG 0.559017 8.3:+25665446 MS.gene28512:CDS
AAGAATCGGCTTTGTCACAT+AGG 0.562883 8.3:-25666102 None:intergenic
GTCTGATATAGAAAACCAAA+GGG 0.569533 8.3:+25665335 MS.gene28512:CDS
ATTCAAATATTTGTAGAACC+TGG 0.571965 8.3:+25665438 MS.gene28512:CDS
TTCCCAGAACCTGATTTGCA+TGG 0.573567 8.3:+25665755 MS.gene28512:CDS
ACATTGGCACAAAGCCGAGA+CGG 0.573840 8.3:+25665671 MS.gene28512:CDS
AGTCTGATATAGAAAACCAA+AGG 0.577396 8.3:+25665334 MS.gene28512:CDS
ATAGCAGTGGAACAGTATCA+AGG 0.585597 8.3:+25665397 MS.gene28512:CDS
CTTTCCTCTTTAATGTACGA+CGG 0.585682 8.3:-25665713 None:intergenic
TTCACAGGGATTCGGAGTAA+GGG 0.591817 8.3:+25667044 MS.gene28512:intron
GGATATCCTTCTACGAGCAC+AGG 0.594050 8.3:-25668000 None:intergenic
ACTGCGTAGTCTTGATTCTG+AGG 0.595824 8.3:+25666130 MS.gene28512:CDS
AAATGGTCGAATCATATTGG+AGG 0.599006 8.3:+25666576 MS.gene28512:CDS
CAGGAGGCACACAACAGACG+CGG 0.600597 8.3:+25667129 MS.gene28512:CDS
TGTGGATCTTTGAAATGCTG+AGG 0.600856 8.3:+25668030 MS.gene28512:CDS
TTGGGAAACCAATGGCCGGA+AGG 0.605290 8.3:+25666539 MS.gene28512:CDS
CATATGTCAAAGACAAGGGA+AGG 0.609395 8.3:+25665484 MS.gene28512:CDS
GATTACAGAGTTATTGTAGA+GGG 0.611271 8.3:+25667751 MS.gene28512:intron
ATATTTGGGAAACCAATGGC+CGG 0.622843 8.3:+25666535 MS.gene28512:CDS
AGATCTACTACACATATCAC+AGG 0.627421 8.3:-25666011 None:intergenic
GGACATATTTGGGAAACCAA+TGG 0.633831 8.3:+25666531 MS.gene28512:CDS
GAAATGATCTCGTATAGCAG+TGG 0.636945 8.3:+25665384 MS.gene28512:CDS
TGGAACAGTATCAAGGCCTG+TGG 0.639274 8.3:+25665404 MS.gene28512:CDS
TTCAAAGATCCACAAAGACA+AGG 0.641053 8.3:-25668021 None:intergenic
TGATTACAGAGTTATTGTAG+AGG 0.655560 8.3:+25667750 MS.gene28512:intron
ACGTTTGTGTGTGAGTACAT+TGG 0.657919 8.3:+25667090 MS.gene28512:CDS
AGGCACACAACAGACGCGGA+AGG 0.658214 8.3:+25667133 MS.gene28512:CDS
ATTGGAGATCAATCCAGTGT+CGG 0.662554 8.3:+25665806 MS.gene28512:CDS
GTAGAATCAATGACATATCG+GGG 0.662628 8.3:-25667320 None:intergenic
ACATAGGTAAACATCTCCCA+AGG 0.667902 8.3:-25666086 None:intergenic
CATATGAGTAGATTGGTCGA+AGG 0.675409 8.3:+25667293 MS.gene28512:CDS
TCGAGCATATGTCAAAGACA+AGG 0.675984 8.3:+25665479 MS.gene28512:CDS
CGAGCATATGTCAAAGACAA+GGG 0.676900 8.3:+25665480 MS.gene28512:CDS
CCAAACAGAATATTGCAGAA+CGG 0.677454 8.3:+25666907 MS.gene28512:CDS
AGGAAAGCTGAAGAAAATTG+CGG 0.686851 8.3:+25665729 MS.gene28512:CDS
GTAAGCACTCCCATTTGTGG+AGG 0.692374 8.3:+25665585 MS.gene28512:CDS
AAAGCTGAAGAAAATTGCGG+TGG 0.708696 8.3:+25665732 MS.gene28512:CDS
TTCGAGTGTAACGATAAGTG+CGG 0.716852 8.3:+25666868 MS.gene28512:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGTAGTTTACAATTAA+TGG - chr8.3:25666612-25666631 None:intergenic 15.0%
!!! TTTTATTTCTCTAAAATAAG+TGG - chr8.3:25667852-25667871 None:intergenic 15.0%
!! ATAGACTACTTTAAACTTAA+CGG + chr8.3:25667612-25667631 MS.gene28512:intron 20.0%
!! GATAAGACAAAAAATTAACA+TGG + chr8.3:25667393-25667412 MS.gene28512:intron 20.0%
!! TGGCATTTAAAAAGAAAAAT+TGG + chr8.3:25666341-25666360 MS.gene28512:intron 20.0%
!! TTGTATTTAACACATTAATC+AGG - chr8.3:25667639-25667658 None:intergenic 20.0%
!!! ATTTTAGCATATTCTTGATA+TGG - chr8.3:25666683-25666702 None:intergenic 20.0%
!!! CTACACATTTAATAACTTTT+TGG - chr8.3:25667530-25667549 None:intergenic 20.0%
!!! GCAATTTGTTCATTTTTAAT+TGG + chr8.3:25667417-25667436 MS.gene28512:intron 20.0%
! AAATTTCTCTACAAAACTTC+AGG + chr8.3:25666831-25666850 MS.gene28512:intron 25.0%
! AATAGCAGGTTAAATATTGT+CGG - chr8.3:25666418-25666437 None:intergenic 25.0%
! AATGCTGTTAAGGAAAAATT+AGG + chr8.3:25665930-25665949 MS.gene28512:CDS 25.0%
! ACCTACAAAATGAATATGTT+AGG - chr8.3:25666706-25666725 None:intergenic 25.0%
! ATGAAGAAAAGGACATATTT+GGG + chr8.3:25666521-25666540 MS.gene28512:CDS 25.0%
! ATGATCAAACTGTAATGATA+CGG + chr8.3:25666180-25666199 MS.gene28512:intron 25.0%
! ATGCTGTTAAGGAAAAATTA+GGG + chr8.3:25665931-25665950 MS.gene28512:CDS 25.0%
! ATTCAAATATTTGTAGAACC+TGG + chr8.3:25665438-25665457 MS.gene28512:CDS 25.0%
! ATTTACAACTAAATGATTGG+TGG + chr8.3:25667688-25667707 MS.gene28512:intron 25.0%
! CGAATATGATATATCGAAAA+TGG + chr8.3:25665275-25665294 MS.gene28512:CDS 25.0%
! GCTATATTTGGTATTAAAAC+AGG - chr8.3:25667228-25667247 None:intergenic 25.0%
! GCTATTTACAACTAAATGAT+TGG + chr8.3:25667685-25667704 MS.gene28512:intron 25.0%
! TAGTAGAATCAATGACATAT+CGG - chr8.3:25667325-25667344 None:intergenic 25.0%
! TGTAAGATGTAAATTCTACT+AGG - chr8.3:25666753-25666772 None:intergenic 25.0%
! TTATTTCCTTGAACATAAGA+TGG + chr8.3:25667462-25667481 MS.gene28512:intron 25.0%
!! ATACCTTTTTGATGAAGAAA+AGG + chr8.3:25666510-25666529 MS.gene28512:CDS 25.0%
!! TCCTAACATATTCATTTTGT+AGG + chr8.3:25666702-25666721 MS.gene28512:intron 25.0%
!! TTTCTTTTTGTAAGAGACAT+TGG + chr8.3:25665655-25665674 MS.gene28512:CDS 25.0%
!!! GCAATTTTCTTTGCTATATT+TGG - chr8.3:25667240-25667259 None:intergenic 25.0%
!!! TAACTAATTTTTTCCAATGC+TGG + chr8.3:25666808-25666827 MS.gene28512:intron 25.0%
!!! TATGTAGTTGTGTTTTGTTA+AGG + chr8.3:25666267-25666286 MS.gene28512:intron 25.0%
AACTCTGTAATCACAAGATT+GGG - chr8.3:25667743-25667762 None:intergenic 30.0%
AGTAGAATCAATGACATATC+GGG - chr8.3:25667324-25667343 None:intergenic 30.0%
AGTCTGATATAGAAAACCAA+AGG + chr8.3:25665334-25665353 MS.gene28512:CDS 30.0%
ATAGCAGGTTAAATATTGTC+GGG - chr8.3:25666417-25666436 None:intergenic 30.0%
CTCTTACAAAAAGAAAATCC+AGG - chr8.3:25665651-25665670 None:intergenic 30.0%
GATGAAGAAAAGGACATATT+TGG + chr8.3:25666520-25666539 MS.gene28512:CDS 30.0%
GATTACAGAGTTATTGTAGA+GGG + chr8.3:25667751-25667770 MS.gene28512:intron 30.0%
GATTGTAAGCATACACATAT+CGG + chr8.3:25667778-25667797 MS.gene28512:CDS 30.0%
GTATATATTGCTACCAATTG+TGG - chr8.3:25665523-25665542 None:intergenic 30.0%
GTCTGATATAGAAAACCAAA+GGG + chr8.3:25665335-25665354 MS.gene28512:CDS 30.0%
TAACATATTCAGAACAGCAT+GGG - chr8.3:25666643-25666662 None:intergenic 30.0%
TAACTCTGTAATCACAAGAT+TGG - chr8.3:25667744-25667763 None:intergenic 30.0%
TACAAAAAGAAAATCCAGGA+CGG - chr8.3:25665647-25665666 None:intergenic 30.0%
TCAAAACCATCTTATGTTCA+AGG - chr8.3:25667471-25667490 None:intergenic 30.0%
TGATTACAGAGTTATTGTAG+AGG + chr8.3:25667750-25667769 MS.gene28512:intron 30.0%
TGTAAATGGTCGAATCATAT+TGG + chr8.3:25666573-25666592 MS.gene28512:CDS 30.0%
TTAACATATTCAGAACAGCA+TGG - chr8.3:25666644-25666663 None:intergenic 30.0%
! ACTCACAAGAATACAGATTT+TGG + chr8.3:25666321-25666340 MS.gene28512:intron 30.0%
! GCAGTAACTTGTAACAATAA+TGG - chr8.3:25667879-25667898 None:intergenic 30.0%
! TGATAATGAGAATGCTGTTA+AGG + chr8.3:25665920-25665939 MS.gene28512:CDS 30.0%
! TGTCCTTTTCTTCATCAAAA+AGG - chr8.3:25666516-25666535 None:intergenic 30.0%
!! GTCATTGATTCTACTACATA+TGG + chr8.3:25667329-25667348 MS.gene28512:CDS 30.0%
!! GTTTTGTTAAGGAGCTAAAT+CGG + chr8.3:25666278-25666297 MS.gene28512:intron 30.0%
!! TTTCTTCCTTTTTACTGACT+TGG + chr8.3:25667202-25667221 MS.gene28512:intron 30.0%
!! TTTTAATTGGCAACATCGTT+AGG + chr8.3:25667430-25667449 MS.gene28512:intron 30.0%
AAATGGTCGAATCATATTGG+AGG + chr8.3:25666576-25666595 MS.gene28512:CDS 35.0%
ACAAAAAGAAAATCCAGGAC+GGG - chr8.3:25665646-25665665 None:intergenic 35.0%
AGATCTACTACACATATCAC+AGG - chr8.3:25666014-25666033 None:intergenic 35.0%
AGGAAAGCTGAAGAAAATTG+CGG + chr8.3:25665729-25665748 MS.gene28512:CDS 35.0%
ATGTATATCTTCCTAAGCCT+TGG + chr8.3:25666069-25666088 MS.gene28512:CDS 35.0%
CAAAAGAAGAGATTACGTCT+TGG - chr8.3:25667171-25667190 None:intergenic 35.0%
CCAAACAGAATATTGCAGAA+CGG + chr8.3:25666907-25666926 MS.gene28512:CDS 35.0%
CTTTATGACAGAGAAAAAGG+TGG + chr8.3:25666951-25666970 MS.gene28512:intron 35.0%
CTTTCCTCTTTAATGTACGA+CGG - chr8.3:25665716-25665735 None:intergenic 35.0%
GAAAAATCACTTCATCCTTG+TGG + chr8.3:25666384-25666403 MS.gene28512:intron 35.0%
GAGATGTTCTTTGCAAAGAA+AGG - chr8.3:25665849-25665868 None:intergenic 35.0%
GGTCTTTATGACAGAGAAAA+AGG + chr8.3:25666948-25666967 MS.gene28512:CDS 35.0%
GTAGAATCAATGACATATCG+GGG - chr8.3:25667323-25667342 None:intergenic 35.0%
TGCTAACCATATGAGTAGAT+TGG + chr8.3:25667286-25667305 MS.gene28512:CDS 35.0%
TGTAGAGAAATTTCCAGCAT+TGG - chr8.3:25666824-25666843 None:intergenic 35.0%
TGTATATCTTCCTAAGCCTT+GGG + chr8.3:25666070-25666089 MS.gene28512:CDS 35.0%
TGTCTAGGTTCATCAATAAC+AGG + chr8.3:25667357-25667376 MS.gene28512:CDS 35.0%
TTATCTGATTCACAGGGATT+CGG + chr8.3:25667036-25667055 MS.gene28512:intron 35.0%
TTCAAAGATCCACAAAGACA+AGG - chr8.3:25668024-25668043 None:intergenic 35.0%
TTCTCTACAAAACTTCAGGA+AGG + chr8.3:25666835-25666854 MS.gene28512:intron 35.0%
TTCTTTGCAAAGAACATCTC+AGG + chr8.3:25665849-25665868 MS.gene28512:CDS 35.0%
!! ACGTTTGTTATCTGATTCAC+AGG + chr8.3:25667029-25667048 MS.gene28512:intron 35.0%
!! CGTTTGTTATCTGATTCACA+GGG + chr8.3:25667030-25667049 MS.gene28512:intron 35.0%
!!! TAACCTGCTATTTTGTTAGC+AGG + chr8.3:25666426-25666445 MS.gene28512:intron 35.0%
AAAGCTGAAGAAAATTGCGG+TGG + chr8.3:25665732-25665751 MS.gene28512:CDS 40.0%
AAAGTAAGCACTCCCATTTG+TGG + chr8.3:25665582-25665601 MS.gene28512:CDS 40.0%
AACAGGCCAAGTCAGTAAAA+AGG - chr8.3:25667211-25667230 None:intergenic 40.0%
ACATAGGTAAACATCTCCCA+AGG - chr8.3:25666089-25666108 None:intergenic 40.0%
ACGTTTGTGTGTGAGTACAT+TGG + chr8.3:25667090-25667109 MS.gene28512:CDS 40.0%
AGGTTCGCATACGATGTAAA+TGG + chr8.3:25666559-25666578 MS.gene28512:CDS 40.0%
ATAGCAGTGGAACAGTATCA+AGG + chr8.3:25665397-25665416 MS.gene28512:CDS 40.0%
ATATTTGGGAAACCAATGGC+CGG + chr8.3:25666535-25666554 MS.gene28512:CDS 40.0%
ATGCAATGACCTAAGCTTTG+CGG + chr8.3:25665977-25665996 MS.gene28512:CDS 40.0%
ATTGGAGATCAATCCAGTGT+CGG + chr8.3:25665806-25665825 MS.gene28512:CDS 40.0%
ATTTGTAGAACCTGGTACCA+AGG + chr8.3:25665446-25665465 MS.gene28512:CDS 40.0%
CAAACCGTCGTACATTAAAG+AGG + chr8.3:25665709-25665728 MS.gene28512:CDS 40.0%
CATATGTCAAAGACAAGGGA+AGG + chr8.3:25665484-25665503 MS.gene28512:CDS 40.0%
CCGTTCTGCAATATTCTGTT+TGG - chr8.3:25666910-25666929 None:intergenic 40.0%
CGAGCATATGTCAAAGACAA+GGG + chr8.3:25665480-25665499 MS.gene28512:CDS 40.0%
CTACATATGGCAATGTGTCT+AGG + chr8.3:25667342-25667361 MS.gene28512:CDS 40.0%
CTGCAATATTCTGTTTGGAC+AGG - chr8.3:25666905-25666924 None:intergenic 40.0%
CTTCGACCAATCTACTCATA+TGG - chr8.3:25667295-25667314 None:intergenic 40.0%
GAAATGATCTCGTATAGCAG+TGG + chr8.3:25665384-25665403 MS.gene28512:CDS 40.0%
GAACGGAGTTCGTGTTAAAT+TGG + chr8.3:25666924-25666943 MS.gene28512:CDS 40.0%
GAAGGATATCCTTGTCTTTG+TGG + chr8.3:25668012-25668031 MS.gene28512:CDS 40.0%
GATCCTGCTAACAAAATAGC+AGG - chr8.3:25666432-25666451 None:intergenic 40.0%
GGACATATTTGGGAAACCAA+TGG + chr8.3:25666531-25666550 MS.gene28512:CDS 40.0%
GTGAACAGTTGACATCAGTA+CGG + chr8.3:25667905-25667924 MS.gene28512:intron 40.0%
TAAGTGGTGTGTGCATATAG+AGG - chr8.3:25667836-25667855 None:intergenic 40.0%
TATACGTGATCACACGTTTC+AGG - chr8.3:25666493-25666512 None:intergenic 40.0%
TATTCAGAACAGCATGGGTT+AGG - chr8.3:25666638-25666657 None:intergenic 40.0%
TCGAGCATATGTCAAAGACA+AGG + chr8.3:25665479-25665498 MS.gene28512:CDS 40.0%
TGAATAGCCATTTCATCCAC+AGG - chr8.3:25665423-25665442 None:intergenic 40.0%
TGTACGACGGTTTGAATTTG+AGG - chr8.3:25665703-25665722 None:intergenic 40.0%
TTCGAGTGTAACGATAAGTG+CGG + chr8.3:25666868-25666887 MS.gene28512:CDS 40.0%
TTGTCGGGAAAAAAACCACA+AGG - chr8.3:25666402-25666421 None:intergenic 40.0%
! AAGAATCGGCTTTGTCACAT+AGG - chr8.3:25666105-25666124 None:intergenic 40.0%
! ATTCAGAACAGCATGGGTTA+GGG - chr8.3:25666637-25666656 None:intergenic 40.0%
! CATATGAGTAGATTGGTCGA+AGG + chr8.3:25667293-25667312 MS.gene28512:CDS 40.0%
! TTGATCTCCAATGGACTTTC+CGG - chr8.3:25665798-25665817 None:intergenic 40.0%
!! CTACGCAGTTGATGAAGAAT+CGG - chr8.3:25666119-25666138 None:intergenic 40.0%
AAAATTAGGGTCGTCGCGAA+AGG + chr8.3:25665944-25665963 MS.gene28512:CDS 45.0%
ACACCAAGTCCATGCAAATC+AGG - chr8.3:25665767-25665786 None:intergenic 45.0%
ACCAGTTGTACCTCCACAAA+TGG - chr8.3:25665598-25665617 None:intergenic 45.0%
AGTCCATGCAAATCAGGTTC+TGG - chr8.3:25665761-25665780 None:intergenic 45.0%
ATCAAGGCCTGTGGATGAAA+TGG + chr8.3:25665413-25665432 MS.gene28512:CDS 45.0%
ATTCACAGGGATTCGGAGTA+AGG + chr8.3:25667043-25667062 MS.gene28512:intron 45.0%
CAGAGTTATTGTAGAGGGCA+CGG + chr8.3:25667756-25667775 MS.gene28512:intron 45.0%
CAGTGTGTGAGAACCACAAT+TGG + chr8.3:25665507-25665526 MS.gene28512:CDS 45.0%
CCAGTTGTACCTCCACAAAT+GGG - chr8.3:25665597-25665616 None:intergenic 45.0%
CGGAGTTCGTGTTAAATTGG+AGG + chr8.3:25666927-25666946 MS.gene28512:CDS 45.0%
GACACTGGATTGATCTCCAA+TGG - chr8.3:25665807-25665826 None:intergenic 45.0%
GCAAGCTAAGTGAATCCCTT+TGG - chr8.3:25665353-25665372 None:intergenic 45.0%
GTAAACATCTCCCAAGGCTT+AGG - chr8.3:25666083-25666102 None:intergenic 45.0%
GTCCATGCAAATCAGGTTCT+GGG - chr8.3:25665760-25665779 None:intergenic 45.0%
TACATCGTATGCGAACCTTC+CGG - chr8.3:25666557-25666576 None:intergenic 45.0%
TTCACAGGGATTCGGAGTAA+GGG + chr8.3:25667044-25667063 MS.gene28512:intron 45.0%
TTGATTCTGCCGCAAAGCTT+AGG - chr8.3:25665989-25666008 None:intergenic 45.0%
! ACATCATTGATGACCGACAC+TGG - chr8.3:25665822-25665841 None:intergenic 45.0%
! ACTGCGTAGTCTTGATTCTG+AGG + chr8.3:25666130-25666149 MS.gene28512:CDS 45.0%
! GAACCTGATTTGCATGGACT+TGG + chr8.3:25665761-25665780 MS.gene28512:CDS 45.0%
! GACGGTTTGAATTTGAGGTC+TGG - chr8.3:25665698-25665717 None:intergenic 45.0%
! TTCCCAGAACCTGATTTGCA+TGG + chr8.3:25665755-25665774 MS.gene28512:CDS 45.0%
!! GATTTGCATGGACTTGGTGT+TGG + chr8.3:25665767-25665786 MS.gene28512:CDS 45.0%
!!! TGAAGTTTTAGAGCAGCAGG+AGG + chr8.3:25667113-25667132 MS.gene28512:CDS 45.0%
!!! TGGTGAAGTTTTAGAGCAGC+AGG + chr8.3:25667110-25667129 MS.gene28512:CDS 45.0%
ACATTGGCACAAAGCCGAGA+CGG + chr8.3:25665671-25665690 MS.gene28512:CDS 50.0%
CATTGTCGATCCTTGGTACC+AGG - chr8.3:25665459-25665478 None:intergenic 50.0%
GGATATCCTTCTACGAGCAC+AGG - chr8.3:25668003-25668022 None:intergenic 50.0%
GGCGAAATGCTCAAGCTTTC+TGG + chr8.3:25665870-25665889 MS.gene28512:CDS 50.0%
GTAAGCACTCCCATTTGTGG+AGG + chr8.3:25665585-25665604 MS.gene28512:CDS 50.0%
TATGAGCCTGTGCTCGTAGA+AGG + chr8.3:25667994-25668013 MS.gene28512:CDS 50.0%
TGCTCGACATTGTCGATCCT+TGG - chr8.3:25665466-25665485 None:intergenic 50.0%
TGGAACAGTATCAAGGCCTG+TGG + chr8.3:25665404-25665423 MS.gene28512:CDS 50.0%
! CCCATTTGTGGAGGTACAAC+TGG + chr8.3:25665594-25665613 MS.gene28512:CDS 50.0%
! TGTAAGTATCGTTCCCGTCC+TGG + chr8.3:25665630-25665649 MS.gene28512:CDS 50.0%
! TGTGGAGGTACAACTGGTTC+TGG + chr8.3:25665600-25665619 MS.gene28512:CDS 50.0%
CATTGGCACAAAGCCGAGAC+GGG + chr8.3:25665672-25665691 MS.gene28512:CDS 55.0%
GGCACATCCGGAAAGTCCAT+TGG + chr8.3:25665788-25665807 MS.gene28512:CDS 55.0%
TATGCGAACCTTCCGGCCAT+TGG - chr8.3:25666550-25666569 None:intergenic 55.0%
TTGGGAAACCAATGGCCGGA+AGG + chr8.3:25666539-25666558 MS.gene28512:CDS 55.0%
! ATTTGAGGTCTGGCCCGTCT+CGG - chr8.3:25665688-25665707 None:intergenic 55.0%
!! GCTGGCGAAGCTATTTTGCG+TGG + chr8.3:25667066-25667085 MS.gene28512:CDS 55.0%
!! GGACTTGGTGTTGGCACATC+CGG + chr8.3:25665776-25665795 MS.gene28512:CDS 55.0%
AGGCACACAACAGACGCGGA+AGG + chr8.3:25667133-25667152 MS.gene28512:CDS 60.0%
CAGGGATTCGGAGTAAGGGC+TGG + chr8.3:25667048-25667067 MS.gene28512:intron 60.0%
! CAGGAGGCACACAACAGACG+CGG + chr8.3:25667129-25667148 MS.gene28512:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 25665231 25668050 25665231 ID=MS.gene28512
chr8.3 mRNA 25665231 25668050 25665231 ID=MS.gene28512.t1;Parent=MS.gene28512
chr8.3 exon 25665231 25666151 25665231 ID=MS.gene28512.t1.exon1;Parent=MS.gene28512.t1
chr8.3 CDS 25665231 25666151 25665231 ID=cds.MS.gene28512.t1;Parent=MS.gene28512.t1
chr8.3 exon 25666448 25666597 25666448 ID=MS.gene28512.t1.exon2;Parent=MS.gene28512.t1
chr8.3 CDS 25666448 25666597 25666448 ID=cds.MS.gene28512.t1;Parent=MS.gene28512.t1
chr8.3 exon 25666853 25666969 25666853 ID=MS.gene28512.t1.exon3;Parent=MS.gene28512.t1
chr8.3 CDS 25666853 25666969 25666853 ID=cds.MS.gene28512.t1;Parent=MS.gene28512.t1
chr8.3 exon 25667051 25667154 25667051 ID=MS.gene28512.t1.exon4;Parent=MS.gene28512.t1
chr8.3 CDS 25667051 25667154 25667051 ID=cds.MS.gene28512.t1;Parent=MS.gene28512.t1
chr8.3 exon 25667247 25667378 25667247 ID=MS.gene28512.t1.exon5;Parent=MS.gene28512.t1
chr8.3 CDS 25667247 25667378 25667247 ID=cds.MS.gene28512.t1;Parent=MS.gene28512.t1
chr8.3 exon 25667759 25667819 25667759 ID=MS.gene28512.t1.exon6;Parent=MS.gene28512.t1
chr8.3 CDS 25667759 25667819 25667759 ID=cds.MS.gene28512.t1;Parent=MS.gene28512.t1
chr8.3 exon 25667958 25668050 25667958 ID=MS.gene28512.t1.exon7;Parent=MS.gene28512.t1
chr8.3 CDS 25667958 25668050 25667958 ID=cds.MS.gene28512.t1;Parent=MS.gene28512.t1
Gene Sequence

>MS.gene28512

ATGGCAATGCTTCATAATGAAGAACATATTTGCGTTGTAAATCGCGAATATGATATATCGAAAATGGATGATGAACTCAGCGACAGTTCAGTTGTAATGAAAGAGTCTGATATAGAAAACCAAAGGGATTCACTTAGCTTGCAAGAATCTACAGAAATGATCTCGTATAGCAGTGGAACAGTATCAAGGCCTGTGGATGAAATGGCTATTCAAATATTTGTAGAACCTGGTACCAAGGATCGACAATGTCGAGCATATGTCAAAGACAAGGGAAGGCAGTGTGTGAGAACCACAATTGGTAGCAATATATACTGTTGTGCTCATTTCTCAAACAAAAGAGAAAGACGTCTTAAAGTAAGCACTCCCATTTGTGGAGGTACAACTGGTTCTGGTAATAGCTGTAAGTATCGTTCCCGTCCTGGATTTTCTTTTTGTAAGAGACATTGGCACAAAGCCGAGACGGGCCAGACCTCAAATTCAAACCGTCGTACATTAAAGAGGAAAGCTGAAGAAAATTGCGGTGGTTCCCAGAACCTGATTTGCATGGACTTGGTGTTGGCACATCCGGAAAGTCCATTGGAGATCAATCCAGTGTCGGTCATCAATGATGTTGATCCTTTCTTTGCAAAGAACATCTCAGGCGAAATGCTCAAGCTTTCTGGTAATGATCATAATGAAGACTCTTGTATTGATAATGAGAATGCTGTTAAGGAAAAATTAGGGTCGTCGCGAAAGGTTATAGTGTTATGCAATGACCTAAGCTTTGCGGCAGAATCAACTCCTGTGATATGTGTAGTAGATCTACAAGTACTAAATTATCTTTGTGAGCAAGAGAGATATGTATATCTTCCTAAGCCTTGGGAGATGTTTACCTATGTGACAAAGCCGATTCTTCATCAACTGCGTAGTCTTGATTCTGAGGTATTTATATCTTTGATTTAATTTTTCTATGATCAAACTGTAATGATACGGCAGTTCATTTCTAGTATACGAAAATAAATGTTTTTTGTTTCTTTTATTTTTCAAAAATAGCTTCTATGTAGTTGTGTTTTGTTAAGGAGCTAAATCGGACGAAATTCTATCATTGAGAACTCACAAGAATACAGATTTTGGCATTTAAAAAGAAAAATTGGAGCTAGAGAATGTTTTCAAAGAAAAATCACTTCATCCTTGTGGTTTTTTTCCCGACAATATTTAACCTGCTATTTTGTTAGCAGGATCTGCAACTGTCTTGTAATTGCTTATATTCTACTTGCTGTCCTGAAACGTGTGATCACGTATACCTTTTTGATGAAGAAAAGGACATATTTGGGAAACCAATGGCCGGAAGGTTCGCATACGATGTAAATGGTCGAATCATATTGGAGGTAAACTATTTCCATTAATTGTAAACTACAATATATCCCTAACCCATGCTGTTCTGAATATGTTAAGCATTGTTTGTTAACACCATATCAAGAATATGCTAAAATCCTAACATATTCATTTTGTAGGTATTTTATTTACTTTTTTAGAATTACCTAGTAGAATTTACATCTTACATTTCTCTTTTCTATTTCTGTATTATTATTAATCTATAACTAATTTTTTCCAATGCTGGAAATTTCTCTACAAAACTTCAGGAAGGTAGTTTTGTCTTCGAGTGTAACGATAAGTGCGGATGCAATAAAACCTGTCCAAACAGAATATTGCAGAACGGAGTTCGTGTTAAATTGGAGGTCTTTATGACAGAGAAAAAGGTGGCAACTATTCTTCTTTTAATAAATTCACAATGACTCTCAAAAAGCTTTTTATGTTGACGTTTGTTATCTGATTCACAGGGATTCGGAGTAAGGGCTGGCGAAGCTATTTTGCGTGGCACGTTTGTGTGTGAGTACATTGGTGAAGTTTTAGAGCAGCAGGAGGCACACAACAGACGCGGAAGGTTTACATTTCTTCCAAGACGTAATCTCTTCTTTTGTATGCTTGATGTTTCTTCCTTTTTACTGACTTGGCCTGTTTTAATACCAAATATAGCAAAGAAAATTGCAGTTATTTCTTAGACATTGATGCTCGTGCTAACCATATGAGTAGATTGGTCGAAGGACATCCCCGATATGTCATTGATTCTACTACATATGGCAATGTGTCTAGGTTCATCAATAACAGGTGAGCATAGATTTGATAAGACAAAAAATTAACATGGTGCAATTTGTTCATTTTTAATTGGCAACATCGTTAGGACTTTGATATTATTTCCTTGAACATAAGATGGTTTTGATATATTCTGAATATGATTAAGCATAAGATTTTATTTCCAAAAAGTTATTAAATGTGTAGAAAATATTTCTAATTTGTTTCAAAGCTGATAAAAGAGTACAAACATTAATAATTAAATTTATATAGACTACTTTAAACTTAACGGACCTGATTAATGTGTTAAATACAATTATCATACATAATTACAAAATAAACGCTATTTACAACTAAATGATTGGTGGTTTGCTATTATTGTTGTTGCAGCTGTTCACCCAATCTTGTGATTACAGAGTTATTGTAGAGGGCACGGATTGTAAGCATACACATATCGGTCTTTACGCAAGTCGAGATGTGAGTATTGTCTCCTCTATATGCACACACCACTTATTTTAGAGAAATAAAAAATTCCATTATTGTTACAAGTTACTGCTTGATTGTGAACAGTTGACATCAGTACGGATTAGATTTGACTTTGTTTCTTGTTAACAGATTGCTTTGCATGAAGAATTGACATATAATTATGACTATGAGCCTGTGCTCGTAGAAGGATATCCTTGTCTTTGTGGATCTTTGAAATGCTGA
Protein sequence

>MS.gene28512.t1

MAMLHNEEHICVVNREYDISKMDDELSDSSVVMKESDIENQRDSLSLQESTEMISYSSGTVSRPVDEMAIQIFVEPGTKDRQCRAYVKDKGRQCVRTTIGSNIYCCAHFSNKRERRLKVSTPICGGTTGSGNSCKYRSRPGFSFCKRHWHKAETGQTSNSNRRTLKRKAEENCGGSQNLICMDLVLAHPESPLEINPVSVINDVDPFFAKNISGEMLKLSGNDHNEDSCIDNENAVKEKLGSSRKVIVLCNDLSFAAESTPVICVVDLQVLNYLCEQERYVYLPKPWEMFTYVTKPILHQLRSLDSEDLQLSCNCLYSTCCPETCDHVYLFDEEKDIFGKPMAGRFAYDVNGRIILEEGSFVFECNDKCGCNKTCPNRILQNGVRVKLEVFMTEKKGFGVRAGEAILRGTFVCEYIGEVLEQQEAHNRRGSKENCSYFLDIDARANHMSRLVEGHPRYVIDSTTYGNVSRFINNRVIVEGTDCKHTHIGLYASRDIALHEELTYNYDYEPVLVEGYPCLCGSLKC