AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28538

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28538.t1 MTR_8g079060 86.831 243 18 5 1 243 1 229 9.95e-123 348
MS.gene28538.t1 MTR_8g015840 74.286 70 17 1 17 85 7 76 5.68e-32 117
MS.gene28538.t1 MTR_4g063780 54.717 106 35 3 17 109 54 159 1.08e-30 116
MS.gene28538.t1 MTR_2g096740 64.000 75 27 0 22 96 55 129 1.17e-30 115
MS.gene28538.t1 MTR_2g096740 64.000 75 27 0 22 96 70 144 1.40e-30 115
MS.gene28538.t1 MTR_4g088580 61.842 76 29 0 31 106 57 132 6.45e-30 115
MS.gene28538.t1 MTR_8g027295 43.511 131 72 1 23 153 18 146 7.14e-30 112
MS.gene28538.t1 MTR_4g022370 40.541 185 86 6 17 191 61 231 2.80e-29 113
MS.gene28538.t1 MTR_4g461080 61.728 81 23 1 1 81 9 81 4.05e-29 110
MS.gene28538.t1 MTR_2g093220 68.056 72 22 1 12 83 24 94 5.34e-29 110
MS.gene28538.t1 MTR_8g479350 58.228 79 33 0 26 104 65 143 5.49e-29 112
MS.gene28538.t1 MTR_7g059400 44.156 154 53 4 17 142 55 203 8.20e-29 111
MS.gene28538.t1 MTR_7g024670 73.770 61 16 0 31 91 79 139 4.21e-28 110
MS.gene28538.t1 MTR_1g077600 62.857 70 26 0 14 83 28 97 5.58e-28 108
MS.gene28538.t1 MTR_1g077600 62.857 70 26 0 14 83 28 97 6.22e-28 107
MS.gene28538.t1 MTR_3g091820 70.588 68 18 2 18 85 33 98 1.01e-27 107
MS.gene28538.t1 MTR_4g089095 75.000 60 14 1 27 86 48 106 3.51e-27 106
MS.gene28538.t1 MTR_4g089095 78.947 57 11 1 27 83 14 69 4.53e-27 105
MS.gene28538.t1 MTR_3g435480 54.023 87 39 1 29 114 136 222 5.12e-27 108
MS.gene28538.t1 MTR_5g031440 71.429 56 16 0 24 79 38 93 1.23e-26 105
MS.gene28538.t1 MTR_8g068210 54.321 81 34 1 29 109 32 109 1.41e-26 105
MS.gene28538.t1 MTR_2g013370 75.439 57 13 1 27 83 44 99 3.23e-26 103
MS.gene28538.t1 MTR_2g014170 83.673 49 8 0 29 77 45 93 5.88e-26 103
MS.gene28538.t1 MTR_3g077750 70.312 64 15 1 16 79 52 111 6.20e-26 103
MS.gene28538.t1 MTR_4g109980 79.245 53 11 0 27 79 38 90 7.10e-26 103
MS.gene28538.t1 MTR_7g010950 38.065 155 72 4 29 179 135 269 7.41e-26 105
MS.gene28538.t1 MTR_2g014060 61.972 71 23 1 10 80 10 76 8.62e-26 103
MS.gene28538.t1 MTR_4g082060 63.934 61 22 0 29 89 122 182 4.79e-25 102
MS.gene28538.t1 MTR_3g090430 47.748 111 45 2 2 112 34 131 4.95e-25 101
MS.gene28538.t1 MTR_2g059540 81.633 49 9 0 29 77 31 79 6.40e-25 98.2
MS.gene28538.t1 MTR_6g012450 37.662 154 77 4 29 177 136 275 8.49e-25 102
MS.gene28538.t1 MTR_2g016030 67.273 55 18 0 26 80 44 98 1.62e-24 95.9
MS.gene28538.t1 MTR_8g044220 62.500 64 23 1 29 92 118 180 3.61e-24 100
MS.gene28538.t1 MTR_5g041420 63.934 61 22 0 21 81 130 190 4.08e-24 98.6
MS.gene28538.t1 MTR_1g056810 51.163 86 36 2 31 110 14 99 4.65e-24 97.8
MS.gene28538.t1 MTR_7g086780 39.655 116 63 2 31 139 101 216 6.62e-24 99.4
MS.gene28538.t1 MTR_6g027460 40.000 130 72 3 29 155 96 222 1.80e-23 97.4
MS.gene28538.t1 MTR_5g041380 73.585 53 14 0 29 81 127 179 4.47e-23 96.7
MS.gene28538.t1 MTR_5g041400 73.585 53 14 0 29 81 119 171 5.54e-23 96.3
MS.gene28538.t1 MTR_6g027450 56.716 67 28 1 29 94 89 155 9.32e-23 95.1
MS.gene28538.t1 MTR_5g041530 62.295 61 23 0 21 81 132 192 1.78e-22 95.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28538.t1 AT3G50410 48.289 263 106 9 1 243 1 253 1.01e-58 186
MS.gene28538.t1 AT5G66940 46.186 236 95 9 4 224 3 221 5.12e-50 163
MS.gene28538.t1 AT1G51700 66.279 86 25 1 24 109 28 109 3.48e-35 124
MS.gene28538.t1 AT1G47655 40.426 235 104 7 11 243 9 209 3.99e-32 117
MS.gene28538.t1 AT5G60850 66.265 83 25 2 27 109 49 128 1.06e-31 118
MS.gene28538.t1 AT3G21270 46.617 133 56 4 29 153 29 154 2.39e-31 115
MS.gene28538.t1 AT5G60200 63.636 88 24 2 22 109 48 127 7.66e-31 115
MS.gene28538.t1 AT3G45610 52.294 109 43 2 2 110 13 112 9.57e-31 114
MS.gene28538.t1 AT1G07640 74.138 58 15 0 29 86 21 78 2.23e-28 108
MS.gene28538.t1 AT3G55370 63.514 74 25 2 21 93 114 186 2.29e-28 110
MS.gene28538.t1 AT3G55370 63.514 74 25 2 21 93 69 141 3.02e-28 109
MS.gene28538.t1 AT3G55370 63.514 74 25 2 21 93 69 141 3.15e-28 110
MS.gene28538.t1 AT5G65590 72.131 61 17 0 23 83 35 95 3.25e-28 109
MS.gene28538.t1 AT1G07640 74.138 58 15 0 29 86 77 134 4.29e-28 109
MS.gene28538.t1 AT1G07640 74.138 58 15 0 29 86 85 142 5.30e-28 109
MS.gene28538.t1 AT1G28310 75.862 58 14 0 29 86 27 84 6.50e-28 108
MS.gene28538.t1 AT5G62430 63.158 76 27 1 29 103 54 129 9.02e-28 107
MS.gene28538.t1 AT1G28310 75.862 58 14 0 29 86 51 108 9.25e-28 108
MS.gene28538.t1 AT4G38000 44.615 130 66 3 10 133 19 148 2.05e-27 105
MS.gene28538.t1 AT3G61850 70.769 65 18 1 23 87 56 119 2.99e-27 106
MS.gene28538.t1 AT3G61850 70.769 65 18 1 23 87 56 119 2.99e-27 106
MS.gene28538.t1 AT5G39660 56.098 82 34 1 29 108 138 219 3.28e-27 108
MS.gene28538.t1 AT5G39660 56.098 82 34 1 29 108 138 219 3.28e-27 108
MS.gene28538.t1 AT5G39660 56.098 82 34 1 29 108 138 219 3.28e-27 108
MS.gene28538.t1 AT3G47500 40.741 135 64 3 1 121 84 216 3.90e-27 108
MS.gene28538.t1 AT4G24060 52.083 96 43 2 18 111 43 137 3.99e-27 107
MS.gene28538.t1 AT5G02460 63.636 77 17 2 17 83 74 149 4.09e-27 107
MS.gene28538.t1 AT3G61850 70.769 65 18 1 23 87 68 131 4.10e-27 106
MS.gene28538.t1 AT3G61850 70.769 65 18 1 23 87 56 119 5.17e-27 106
MS.gene28538.t1 AT3G61850 70.769 65 18 1 23 87 68 131 5.70e-27 106
MS.gene28538.t1 AT2G28810 73.684 57 15 0 24 80 72 128 8.30e-27 105
MS.gene28538.t1 AT2G28810 73.684 57 15 0 24 80 89 145 1.14e-26 105
MS.gene28538.t1 AT2G28510 38.690 168 66 4 31 163 50 215 2.00e-26 104
MS.gene28538.t1 AT2G37590 59.722 72 29 0 12 83 72 143 4.45e-26 103
MS.gene28538.t1 AT4G21050 64.179 67 23 1 31 97 26 91 1.66e-25 99.8
MS.gene28538.t1 AT1G21340 64.706 68 21 1 25 89 31 98 2.04e-25 100
MS.gene28538.t1 AT4G00940 70.690 58 17 0 26 83 65 122 4.46e-25 100
MS.gene28538.t1 AT4G00940 70.690 58 17 0 26 83 65 122 4.46e-25 100
MS.gene28538.t1 AT4G00940 70.690 58 17 0 26 83 65 122 4.46e-25 100
MS.gene28538.t1 AT5G62940 67.188 64 17 1 16 79 64 123 1.16e-24 100
MS.gene28538.t1 AT3G52440 63.636 66 24 0 18 83 14 79 1.22e-24 98.6
MS.gene28538.t1 AT1G26790 60.000 70 27 1 29 97 111 180 1.48e-24 100
MS.gene28538.t1 AT3G52440 63.636 66 24 0 18 83 33 98 1.61e-24 98.6
MS.gene28538.t1 AT2G46590 74.074 54 14 0 23 76 62 115 1.77e-24 100
MS.gene28538.t1 AT2G46590 74.074 54 14 0 23 76 74 127 1.96e-24 100
MS.gene28538.t1 AT1G29160 45.000 100 55 0 29 128 62 161 2.99e-24 95.5
MS.gene28538.t1 AT4G21040 62.687 67 23 1 31 97 27 91 5.94e-24 96.3
MS.gene28538.t1 AT1G69570 69.811 53 16 0 29 81 132 184 6.93e-24 99.0
MS.gene28538.t1 AT4G21080 64.179 67 22 1 31 97 27 91 2.10e-23 95.1
MS.gene28538.t1 AT1G64620 60.811 74 20 2 14 78 25 98 3.97e-23 96.3
MS.gene28538.t1 AT2G34140 67.925 53 17 0 29 81 58 110 6.55e-23 92.0
MS.gene28538.t1 AT4G21030 60.000 50 20 0 31 80 23 72 1.95e-13 67.4

Find 89 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCAAAACTCTGGGTTTC+TGG 0.121250 8.4:+27719038 MS.gene28538:CDS
TTCTGGCATTAGGTGGATTT+GGG 0.223026 8.4:+27719055 MS.gene28538:CDS
CCGTCAATACCGCCGGAAAA+TGG 0.237022 8.4:-27718952 None:intergenic
GGTGTTCCTGTTGTGTCTTC+TGG 0.239637 8.4:+27719168 MS.gene28538:CDS
TTTCTGGCATTAGGTGGATT+TGG 0.249923 8.4:+27719054 MS.gene28538:CDS
CATTAGGTGGATTTGGGCTT+GGG 0.256673 8.4:+27719061 MS.gene28538:CDS
GCTATTTCAACACCAGTAAA+TGG 0.284985 8.4:+27719273 MS.gene28538:CDS
TCCTCCTTGGACGGCGGAGA+TGG 0.289163 8.4:-27718920 None:intergenic
GCATTAGGTGGATTTGGGCT+TGG 0.292718 8.4:+27719060 MS.gene28538:CDS
GGTGGATTTGGGCTTGGGCT+TGG 0.292816 8.4:+27719066 MS.gene28538:CDS
TTTGGGCTTGGGCTTGGACA+TGG 0.300293 8.4:+27719072 MS.gene28538:CDS
GCAAAGCTTGTCGCCGCTAC+TGG 0.340843 8.4:+27718740 MS.gene28538:CDS
TTGGGCTTGGGCTTGGACAT+GGG 0.342240 8.4:+27719073 MS.gene28538:CDS
TTTGATCCATCCACCATTCC+TGG 0.347481 8.4:-27719141 None:intergenic
GGGCTTGAAGAAATGGGCTT+TGG 0.347490 8.4:+27719093 MS.gene28538:CDS
GTTGTGTCTTCTGGTGTTGT+TGG 0.348865 8.4:+27719177 MS.gene28538:CDS
AAGAAATGGGCTTTGGGATT+GGG 0.351153 8.4:+27719100 MS.gene28538:CDS
TGCTCCGGTGCCGGTGCACC+GGG 0.353622 8.4:-27718628 None:intergenic
TGGACATGGGCTTGAAGAAA+TGG 0.357925 8.4:+27719086 MS.gene28538:CDS
TGGGATTGGGAGAACAGGTT+GGG 0.361604 8.4:+27719113 MS.gene28538:CDS
GGTGATTGTTTCTCTTGGCC+TGG 0.369523 8.4:+27719246 MS.gene28538:CDS
GGCTTTGGGATTGGGAGAAC+AGG 0.373009 8.4:+27719108 MS.gene28538:CDS
TTGCTCCGGTGCCGGTGCAC+CGG 0.375742 8.4:-27718629 None:intergenic
GGCTTGAAGAAATGGGCTTT+GGG 0.379257 8.4:+27719094 MS.gene28538:CDS
GTTCATTTGAGACCATTTAC+TGG 0.380286 8.4:-27719285 None:intergenic
GAAGAAATGGGCTTTGGGAT+TGG 0.382875 8.4:+27719099 MS.gene28538:CDS
GTGGTGGTGATTGTTTCTCT+TGG 0.384765 8.4:+27719241 MS.gene28538:CDS
CCTCCTTGGACGGCGGAGAT+GGG 0.390226 8.4:-27718919 None:intergenic
TGTTGATCGGCGGCTTTCAC+CGG 0.408674 8.4:-27718590 None:intergenic
GGTGCACCGGGGCGGATTTG+AGG 0.417132 8.4:-27718616 None:intergenic
AACTCTGGGTTTCTGGCATT+AGG 0.420912 8.4:+27719045 MS.gene28538:CDS
CCGCCCCGGTGCACCGGCAC+CGG 0.422541 8.4:+27718624 MS.gene28538:CDS
TGCCGTGACTTCAGCGGCGC+CGG 0.425658 8.4:+27718870 MS.gene28538:CDS
AACAACAACAATCAAAACTC+TGG 0.431082 8.4:+27719030 MS.gene28538:CDS
AGATTCTCTTGCTCCGGTGC+CGG 0.436640 8.4:-27718637 None:intergenic
ATCTCCGCCGTCCAAGGAGG+AGG 0.437854 8.4:+27718922 MS.gene28538:CDS
GGACATGGGCTTGAAGAAAT+GGG 0.438047 8.4:+27719087 MS.gene28538:CDS
TTGGGATTGGGAGAACAGGT+TGG 0.446014 8.4:+27719112 MS.gene28538:CDS
ATGCTGAGTACGAGAGCGTT+TGG 0.449036 8.4:-27718818 None:intergenic
ACAACACCAGAAGACACAAC+AGG 0.458334 8.4:-27719174 None:intergenic
CCGGCGGTATTGACGGTGAT+GGG 0.466545 8.4:+27718959 MS.gene28538:CDS
GGAAACACGTGGCAGTTAGA+AGG 0.471025 8.4:+27719198 MS.gene28538:CDS
ATGGAAAAGACCCTCCTCCT+TGG 0.474800 8.4:-27718933 None:intergenic
CTCCGGCGCCGCTGAAGTCA+CGG 0.478425 8.4:-27718872 None:intergenic
TTTCTGGTACCACCGCCGAC+GGG 0.484650 8.4:-27718790 None:intergenic
CGTGACATCCCCGTCGGCGG+TGG 0.490474 8.4:+27718781 MS.gene28538:CDS
CATGGGAGATTCTCTTGCTC+CGG 0.492080 8.4:-27718643 None:intergenic
CAGTTAGAAGGTGGTGAAGG+TGG 0.495978 8.4:+27719210 MS.gene28538:CDS
TCTCTAAACAATGACATCTT+CGG 0.498140 8.4:+27718564 None:intergenic
TCAAATCCGCCCCGGTGCAC+CGG 0.515212 8.4:+27718618 MS.gene28538:CDS
TCCGGCGGTATTGACGGTGA+TGG 0.517628 8.4:+27718958 MS.gene28538:CDS
CGCCGCTGAAGTCACGGCAG+AGG 0.520050 8.4:-27718866 None:intergenic
AAGCAGAATATGAGTGTTTG+TGG 0.522514 8.4:+27718982 MS.gene28538:CDS
GCTTGATAGTGGTGGTGCTC+CGG 0.532605 8.4:-27718889 None:intergenic
GTTGAATCACAACGTGGACA+TGG 0.538502 8.4:-27718661 None:intergenic
TCTGGGTTTCTGGCATTAGG+TGG 0.547157 8.4:+27719048 MS.gene28538:CDS
CACCGCCGACGGGGATGTCA+CGG 0.552285 8.4:-27718780 None:intergenic
CACGGCAGAGGAAGAAGATG+AGG 0.555442 8.4:-27718854 None:intergenic
GTAGTAACAAAACTTAGTGT+TGG 0.560310 8.4:-27718683 None:intergenic
ACACCCATCTCCGCCGTCCA+AGG 0.561242 8.4:+27718916 MS.gene28538:CDS
TTCTGGTACCACCGCCGACG+GGG 0.573655 8.4:-27718789 None:intergenic
ACAACAACAATCAAAACTCT+GGG 0.575835 8.4:+27719031 MS.gene28538:CDS
CCCATCACCGTCAATACCGC+CGG 0.580375 8.4:-27718959 None:intergenic
TCTCCGCCGTCCAAGGAGGA+GGG 0.582119 8.4:+27718923 MS.gene28538:CDS
ACAATGACATCTTCGGACTC+CGG 0.583202 8.4:+27718571 None:intergenic
GGTGTTGAAATAGCAAGTCC+AGG 0.592777 8.4:-27719264 None:intergenic
TGGGTGTCATTGCTTGATAG+TGG 0.598785 8.4:-27718900 None:intergenic
CCGGTGCCGGTGCACCGGGG+CGG 0.610074 8.4:-27718624 None:intergenic
AGACCCTCCTCCTTGGACGG+CGG 0.612466 8.4:-27718926 None:intergenic
CTCCGTGACATCCCCGTCGG+CGG 0.612747 8.4:+27718778 MS.gene28538:CDS
TTCCTCTGCCGTGACTTCAG+CGG 0.618657 8.4:+27718864 MS.gene28538:CDS
TGTCGCCGCTACTGGACTCA+CGG 0.619346 8.4:+27718748 MS.gene28538:CDS
ACAAAACCTCAAATCCGCCC+CGG 0.621584 8.4:+27718610 MS.gene28538:CDS
TGGCAGTTAGAAGGTGGTGA+AGG 0.635231 8.4:+27719207 MS.gene28538:CDS
CAAGCTTTGCAGAAATGACG+TGG 0.636873 8.4:-27718727 None:intergenic
ACCCTCCGTGACATCCCCGT+CGG 0.638321 8.4:+27718775 MS.gene28538:CDS
AAAAGACCCTCCTCCTTGGA+CGG 0.641146 8.4:-27718929 None:intergenic
TTGAATCACAACGTGGACAT+GGG 0.644805 8.4:-27718660 None:intergenic
GTGTCATTGCTTGATAGTGG+TGG 0.649877 8.4:-27718897 None:intergenic
CCCATCTCCGCCGTCCAAGG+AGG 0.650434 8.4:+27718919 MS.gene28538:CDS
TACCACCGTGAGTCCAGTAG+CGG 0.656577 8.4:-27718753 None:intergenic
GCTCCGGTGCCGGTGCACCG+GGG 0.657998 8.4:-27718627 None:intergenic
GCCGACGGGGATGTCACGGA+GGG 0.667751 8.4:-27718776 None:intergenic
AACACGTGGCAGTTAGAAGG+TGG 0.673819 8.4:+27719201 MS.gene28538:CDS
CGCCGACGGGGATGTCACGG+AGG 0.674019 8.4:-27718777 None:intergenic
ATGGTGGATGGATCAAACAT+TGG 0.699118 8.4:+27719147 MS.gene28538:CDS
CTGGTGTTGTTGGAAACACG+TGG 0.703407 8.4:+27719187 MS.gene28538:CDS
CGCCGCTACTGGACTCACGG+TGG 0.745530 8.4:+27718751 MS.gene28538:CDS
GTGTTGGTTGAATCACAACG+TGG 0.795975 8.4:-27718667 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACAACAACAATCAAAACTC+TGG + chr8.4:27719030-27719049 MS.gene28538:CDS 30.0%
ACAACAACAATCAAAACTCT+GGG + chr8.4:27719031-27719050 MS.gene28538:CDS 30.0%
GTAGTAACAAAACTTAGTGT+TGG - chr8.4:27718686-27718705 None:intergenic 30.0%
AAGCAGAATATGAGTGTTTG+TGG + chr8.4:27718982-27719001 MS.gene28538:CDS 35.0%
! GCTATTTCAACACCAGTAAA+TGG + chr8.4:27719273-27719292 MS.gene28538:CDS 35.0%
!!! GGATTTGAGGTTTTGTTGAT+CGG - chr8.4:27718606-27718625 None:intergenic 35.0%
CAATCAAAACTCTGGGTTTC+TGG + chr8.4:27719038-27719057 MS.gene28538:CDS 40.0%
TTGAATCACAACGTGGACAT+GGG - chr8.4:27718663-27718682 None:intergenic 40.0%
! TTCTGGCATTAGGTGGATTT+GGG + chr8.4:27719055-27719074 MS.gene28538:CDS 40.0%
! TTTCTGGCATTAGGTGGATT+TGG + chr8.4:27719054-27719073 MS.gene28538:CDS 40.0%
!! AAGAAATGGGCTTTGGGATT+GGG + chr8.4:27719100-27719119 MS.gene28538:CDS 40.0%
!! ATGGTGGATGGATCAAACAT+TGG + chr8.4:27719147-27719166 MS.gene28538:CDS 40.0%
!!! TTTGAGGTTTTGTTGATCGG+CGG - chr8.4:27718603-27718622 None:intergenic 40.0%
ACAACACCAGAAGACACAAC+AGG - chr8.4:27719177-27719196 None:intergenic 45.0%
CAAGCTTTGCAGAAATGACG+TGG - chr8.4:27718730-27718749 None:intergenic 45.0%
GGACATGGGCTTGAAGAAAT+GGG + chr8.4:27719087-27719106 MS.gene28538:CDS 45.0%
GTTGAATCACAACGTGGACA+TGG - chr8.4:27718664-27718683 None:intergenic 45.0%
TGGACATGGGCTTGAAGAAA+TGG + chr8.4:27719086-27719105 MS.gene28538:CDS 45.0%
TTTGATCCATCCACCATTCC+TGG - chr8.4:27719144-27719163 None:intergenic 45.0%
! CATTAGGTGGATTTGGGCTT+GGG + chr8.4:27719061-27719080 MS.gene28538:CDS 45.0%
! GGAGGGTCTTTTCCATTTTC+CGG + chr8.4:27718940-27718959 MS.gene28538:CDS 45.0%
! GGCTTGAAGAAATGGGCTTT+GGG + chr8.4:27719094-27719113 MS.gene28538:CDS 45.0%
! GGTGTTGAAATAGCAAGTCC+AGG - chr8.4:27719267-27719286 None:intergenic 45.0%
! GTGGTGGTGATTGTTTCTCT+TGG + chr8.4:27719241-27719260 MS.gene28538:CDS 45.0%
!! AACTCTGGGTTTCTGGCATT+AGG + chr8.4:27719045-27719064 MS.gene28538:CDS 45.0%
!! GAAGAAATGGGCTTTGGGAT+TGG + chr8.4:27719099-27719118 MS.gene28538:CDS 45.0%
!! GTGTCATTGCTTGATAGTGG+TGG - chr8.4:27718900-27718919 None:intergenic 45.0%
!! GTGTTGGTTGAATCACAACG+TGG - chr8.4:27718670-27718689 None:intergenic 45.0%
!! GTTGTGTCTTCTGGTGTTGT+TGG + chr8.4:27719177-27719196 MS.gene28538:CDS 45.0%
!! TGGGTGTCATTGCTTGATAG+TGG - chr8.4:27718903-27718922 None:intergenic 45.0%
!!! AGAGCGTTTGGCGTTTTTTC+TGG - chr8.4:27718809-27718828 None:intergenic 45.0%
AAAAGACCCTCCTCCTTGGA+CGG - chr8.4:27718932-27718951 None:intergenic 50.0%
AACACGTGGCAGTTAGAAGG+TGG + chr8.4:27719201-27719220 MS.gene28538:CDS 50.0%
ACAAAACCTCAAATCCGCCC+CGG + chr8.4:27718610-27718629 MS.gene28538:CDS 50.0%
ATGGAAAAGACCCTCCTCCT+TGG - chr8.4:27718936-27718955 None:intergenic 50.0%
CAGTTAGAAGGTGGTGAAGG+TGG + chr8.4:27719210-27719229 MS.gene28538:CDS 50.0%
CATGGGAGATTCTCTTGCTC+CGG - chr8.4:27718646-27718665 None:intergenic 50.0%
GGAAACACGTGGCAGTTAGA+AGG + chr8.4:27719198-27719217 MS.gene28538:CDS 50.0%
GGTGTTCCTGTTGTGTCTTC+TGG + chr8.4:27719168-27719187 MS.gene28538:CDS 50.0%
TGGCAGTTAGAAGGTGGTGA+AGG + chr8.4:27719207-27719226 MS.gene28538:CDS 50.0%
TGGGATTGGGAGAACAGGTT+GGG + chr8.4:27719113-27719132 MS.gene28538:CDS 50.0%
TTGGGATTGGGAGAACAGGT+TGG + chr8.4:27719112-27719131 MS.gene28538:CDS 50.0%
! AGGTTGGGCTTTTCCAGGAA+TGG + chr8.4:27719128-27719147 MS.gene28538:CDS 50.0%
! GCATTAGGTGGATTTGGGCT+TGG + chr8.4:27719060-27719079 MS.gene28538:CDS 50.0%
! GGGCTTGAAGAAATGGGCTT+TGG + chr8.4:27719093-27719112 MS.gene28538:CDS 50.0%
! GGGTCTTTTCCATTTTCCGG+CGG + chr8.4:27718943-27718962 MS.gene28538:CDS 50.0%
! GGTGATTGTTTCTCTTGGCC+TGG + chr8.4:27719246-27719265 MS.gene28538:CDS 50.0%
! TTGGGCTTTTCCAGGAATGG+TGG + chr8.4:27719131-27719150 MS.gene28538:CDS 50.0%
!! AGAACAGGTTGGGCTTTTCC+AGG + chr8.4:27719123-27719142 MS.gene28538:CDS 50.0%
!! ATGCTGAGTACGAGAGCGTT+TGG - chr8.4:27718821-27718840 None:intergenic 50.0%
!! CCATTTTCCGGCGGTATTGA+CGG + chr8.4:27718952-27718971 MS.gene28538:CDS 50.0%
!! CTGGTGTTGTTGGAAACACG+TGG + chr8.4:27719187-27719206 MS.gene28538:CDS 50.0%
!! GCTTTTCCAGGAATGGTGGA+TGG + chr8.4:27719135-27719154 MS.gene28538:CDS 50.0%
!! TCTGGGTTTCTGGCATTAGG+TGG + chr8.4:27719048-27719067 MS.gene28538:CDS 50.0%
!!! GAAGGTGGTTTTGTTGGTGG+TGG + chr8.4:27719225-27719244 MS.gene28538:CDS 50.0%
!!! GGTGAAGGTGGTTTTGTTGG+TGG + chr8.4:27719222-27719241 MS.gene28538:CDS 50.0%
!!! GGTGGTGAAGGTGGTTTTGT+TGG + chr8.4:27719219-27719238 MS.gene28538:CDS 50.0%
AGATTCTCTTGCTCCGGTGC+CGG - chr8.4:27718640-27718659 None:intergenic 55.0%
CACGGCAGAGGAAGAAGATG+AGG - chr8.4:27718857-27718876 None:intergenic 55.0%
CCGTCAATACCGCCGGAAAA+TGG - chr8.4:27718955-27718974 None:intergenic 55.0%
TACCACCGTGAGTCCAGTAG+CGG - chr8.4:27718756-27718775 None:intergenic 55.0%
TGTTGATCGGCGGCTTTCAC+CGG - chr8.4:27718593-27718612 None:intergenic 55.0%
TTCCTCTGCCGTGACTTCAG+CGG + chr8.4:27718864-27718883 MS.gene28538:CDS 55.0%
TTGGGCTTGGGCTTGGACAT+GGG + chr8.4:27719073-27719092 MS.gene28538:CDS 55.0%
TTTGGGCTTGGGCTTGGACA+TGG + chr8.4:27719072-27719091 MS.gene28538:CDS 55.0%
!! GCTTGATAGTGGTGGTGCTC+CGG - chr8.4:27718892-27718911 None:intergenic 55.0%
!! GGCTTTGGGATTGGGAGAAC+AGG + chr8.4:27719108-27719127 MS.gene28538:CDS 55.0%
!! TTTTCTGGTACCACCGCCGA+CGG - chr8.4:27718794-27718813 None:intergenic 55.0%
CCCATCACCGTCAATACCGC+CGG - chr8.4:27718962-27718981 None:intergenic 60.0%
GCAAAGCTTGTCGCCGCTAC+TGG + chr8.4:27718740-27718759 MS.gene28538:CDS 60.0%
TGTCGCCGCTACTGGACTCA+CGG + chr8.4:27718748-27718767 MS.gene28538:CDS 60.0%
! GGTGGATTTGGGCTTGGGCT+TGG + chr8.4:27719066-27719085 MS.gene28538:CDS 60.0%
! TTTCTGGTACCACCGCCGAC+GGG - chr8.4:27718793-27718812 None:intergenic 60.0%
!! CCGGCGGTATTGACGGTGAT+GGG + chr8.4:27718959-27718978 MS.gene28538:CDS 60.0%
!! TCCGGCGGTATTGACGGTGA+TGG + chr8.4:27718958-27718977 MS.gene28538:CDS 60.0%
ACACCCATCTCCGCCGTCCA+AGG + chr8.4:27718916-27718935 MS.gene28538:CDS 65.0%
ACCCTCCGTGACATCCCCGT+CGG + chr8.4:27718775-27718794 MS.gene28538:CDS 65.0%
AGACCCTCCTCCTTGGACGG+CGG - chr8.4:27718929-27718948 None:intergenic 65.0%
ATCTCCGCCGTCCAAGGAGG+AGG + chr8.4:27718922-27718941 MS.gene28538:CDS 65.0%
CCTCCTTGGACGGCGGAGAT+GGG - chr8.4:27718922-27718941 None:intergenic 65.0%
TCAAATCCGCCCCGGTGCAC+CGG + chr8.4:27718618-27718637 MS.gene28538:CDS 65.0%
TCCTCCTTGGACGGCGGAGA+TGG - chr8.4:27718923-27718942 None:intergenic 65.0%
TCTCCGCCGTCCAAGGAGGA+GGG + chr8.4:27718923-27718942 MS.gene28538:CDS 65.0%
TTCTGGTACCACCGCCGACG+GGG - chr8.4:27718792-27718811 None:intergenic 65.0%
CACCGCCGACGGGGATGTCA+CGG - chr8.4:27718783-27718802 None:intergenic 70.0%
CCCATCTCCGCCGTCCAAGG+AGG + chr8.4:27718919-27718938 MS.gene28538:CDS 70.0%
CGCCGCTACTGGACTCACGG+TGG + chr8.4:27718751-27718770 MS.gene28538:CDS 70.0%
CGCCGCTGAAGTCACGGCAG+AGG - chr8.4:27718869-27718888 None:intergenic 70.0%
CTCCGGCGCCGCTGAAGTCA+CGG - chr8.4:27718875-27718894 None:intergenic 70.0%
CTCCGTGACATCCCCGTCGG+CGG + chr8.4:27718778-27718797 MS.gene28538:CDS 70.0%
GCCGACGGGGATGTCACGGA+GGG - chr8.4:27718779-27718798 None:intergenic 70.0%
TGCCGTGACTTCAGCGGCGC+CGG + chr8.4:27718870-27718889 MS.gene28538:CDS 70.0%
! GGTGCACCGGGGCGGATTTG+AGG - chr8.4:27718619-27718638 None:intergenic 70.0%
!! TTGCTCCGGTGCCGGTGCAC+CGG - chr8.4:27718632-27718651 None:intergenic 70.0%
CGCCGACGGGGATGTCACGG+AGG - chr8.4:27718780-27718799 None:intergenic 75.0%
CGTGACATCCCCGTCGGCGG+TGG + chr8.4:27718781-27718800 MS.gene28538:CDS 75.0%
!! TGCTCCGGTGCCGGTGCACC+GGG - chr8.4:27718631-27718650 None:intergenic 75.0%
!!! GCTCCGGTGCCGGTGCACCG+GGG - chr8.4:27718630-27718649 None:intergenic 80.0%
!! CCGCCCCGGTGCACCGGCAC+CGG + chr8.4:27718624-27718643 MS.gene28538:CDS 85.0%
!!! CCGGTGCCGGTGCACCGGGG+CGG - chr8.4:27718627-27718646 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 27718574 27719305 27718574 ID=MS.gene28538
chr8.4 mRNA 27718574 27719305 27718574 ID=MS.gene28538.t1;Parent=MS.gene28538
chr8.4 exon 27718574 27719305 27718574 ID=MS.gene28538.t1.exon1;Parent=MS.gene28538.t1
chr8.4 CDS 27718574 27719305 27718574 ID=cds.MS.gene28538.t1;Parent=MS.gene28538.t1
Gene Sequence

>MS.gene28538

ATGACATCTTCGGACTCCGGTGAAAGCCGCCGATCAACAAAACCTCAAATCCGCCCCGGTGCACCGGCACCGGAGCAAGAGAATCTCCCATGTCCACGTTGTGATTCAACCAACACTAAGTTTTGTTACTACAACAACTACAACTACTCTCAGCCACGTCATTTCTGCAAAGCTTGTCGCCGCTACTGGACTCACGGTGGTACCCTCCGTGACATCCCCGTCGGCGGTGGTACCAGAAAAAACGCCAAACGCTCTCGTACTCAGCATGTTGCTGTCACTTCCTCATCTTCTTCCTCTGCCGTGACTTCAGCGGCGCCGGAGCACCACCACTATCAAGCAATGACACCCATCTCCGCCGTCCAAGGAGGAGGGTCTTTTCCATTTTCCGGCGGTATTGACGGTGATGGGAAGCAGAATATGAGTGTTTGTGGAAGCTTCACTTCATTGCTGAACAACAACAACAACAATCAAAACTCTGGGTTTCTGGCATTAGGTGGATTTGGGCTTGGGCTTGGACATGGGCTTGAAGAAATGGGCTTTGGGATTGGGAGAACAGGTTGGGCTTTTCCAGGAATGGTGGATGGATCAAACATTGGTGTTCCTGTTGTGTCTTCTGGTGTTGTTGGAAACACGTGGCAGTTAGAAGGTGGTGAAGGTGGTTTTGTTGGTGGTGGTGATTGTTTCTCTTGGCCTGGACTTGCTATTTCAACACCAGTAAATGGTCTCAAATGA
Protein sequence

>MS.gene28538.t1

MTSSDSGESRRSTKPQIRPGAPAPEQENLPCPRCDSTNTKFCYYNNYNYSQPRHFCKACRRYWTHGGTLRDIPVGGGTRKNAKRSRTQHVAVTSSSSSSAVTSAAPEHHHYQAMTPISAVQGGGSFPFSGGIDGDGKQNMSVCGSFTSLLNNNNNNQNSGFLALGGFGLGLGHGLEEMGFGIGRTGWAFPGMVDGSNIGVPVVSSGVVGNTWQLEGGEGGFVGGGDCFSWPGLAISTPVNGLK