AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28547

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28547.t1 MTR_8g479350 89.773 352 26 4 1 351 1 343 0.0 561
MS.gene28547.t1 MTR_4g063780 68.354 79 16 1 71 140 72 150 3.69e-33 125
MS.gene28547.t1 MTR_2g096740 76.190 63 15 0 74 136 62 124 4.02e-33 124
MS.gene28547.t1 MTR_2g096740 76.190 63 15 0 74 136 77 139 4.34e-33 125
MS.gene28547.t1 MTR_7g024670 52.212 113 41 2 39 138 29 141 1.69e-32 125
MS.gene28547.t1 MTR_8g068210 55.556 90 40 0 61 150 19 108 4.35e-31 120
MS.gene28547.t1 MTR_5g031440 92.157 51 4 0 74 124 43 93 5.42e-31 120
MS.gene28547.t1 MTR_4g088580 79.661 59 12 0 75 133 56 114 7.89e-31 120
MS.gene28547.t1 MTR_3g090430 59.036 83 30 1 49 127 22 104 1.65e-30 119
MS.gene28547.t1 MTR_7g059400 78.947 57 12 0 71 127 73 129 2.46e-30 118
MS.gene28547.t1 MTR_8g079060 57.143 84 36 0 66 149 21 104 5.43e-30 114
MS.gene28547.t1 MTR_8g027295 63.158 76 28 0 71 146 21 96 5.69e-30 115
MS.gene28547.t1 MTR_3g091820 62.338 77 29 0 51 127 20 96 9.58e-30 116
MS.gene28547.t1 MTR_4g461080 84.906 53 8 0 74 126 29 81 1.51e-29 115
MS.gene28547.t1 MTR_4g022370 81.481 54 10 0 74 127 82 135 1.75e-29 116
MS.gene28547.t1 MTR_4g109980 86.275 51 7 0 74 124 40 90 2.41e-29 115
MS.gene28547.t1 MTR_8g015840 80.000 55 11 0 71 125 17 71 3.35e-29 112
MS.gene28547.t1 MTR_2g093220 85.185 54 8 0 74 127 40 93 5.20e-29 114
MS.gene28547.t1 MTR_1g056810 81.034 58 11 0 70 127 8 65 1.73e-28 112
MS.gene28547.t1 MTR_2g014060 83.636 55 8 1 71 125 23 76 2.80e-28 112
MS.gene28547.t1 MTR_3g077750 53.684 95 39 2 33 124 19 111 3.99e-28 112
MS.gene28547.t1 MTR_4g089095 78.947 57 11 1 73 129 49 104 9.65e-28 110
MS.gene28547.t1 MTR_4g089095 78.947 57 11 1 73 129 15 70 1.07e-27 109
MS.gene28547.t1 MTR_2g059540 85.714 49 7 0 74 122 31 79 2.19e-27 107
MS.gene28547.t1 MTR_1g077600 72.414 58 16 0 69 126 38 95 7.90e-27 107
MS.gene28547.t1 MTR_2g014170 83.673 49 8 0 74 122 45 93 7.93e-27 108
MS.gene28547.t1 MTR_1g077600 72.414 58 16 0 69 126 38 95 8.03e-27 107
MS.gene28547.t1 MTR_2g016030 50.515 97 42 2 76 172 49 139 1.46e-26 103
MS.gene28547.t1 MTR_2g013370 73.214 56 14 1 74 129 46 100 1.20e-25 104
MS.gene28547.t1 MTR_6g012450 75.926 54 13 0 74 127 136 189 2.99e-25 106
MS.gene28547.t1 MTR_7g010950 43.103 116 49 2 30 128 74 189 4.70e-25 105
MS.gene28547.t1 MTR_7g086780 57.895 76 32 0 74 149 99 174 7.26e-25 104
MS.gene28547.t1 MTR_6g027460 35.673 171 95 2 45 205 67 232 7.44e-25 103
MS.gene28547.t1 MTR_3g435480 72.222 54 15 0 74 127 136 189 1.38e-24 104
MS.gene28547.t1 MTR_5g041420 52.041 98 40 2 44 136 105 200 1.48e-24 102
MS.gene28547.t1 MTR_6g027450 49.412 85 43 0 45 129 60 144 1.68e-24 102
MS.gene28547.t1 MTR_8g044220 56.164 73 32 0 74 146 118 190 1.78e-24 103
MS.gene28547.t1 MTR_4g082060 66.102 59 20 0 74 132 122 180 1.79e-24 103
MS.gene28547.t1 MTR_5g041530 67.188 64 20 1 76 139 142 204 1.29e-23 100
MS.gene28547.t1 MTR_5g041400 49.462 93 45 1 46 136 89 181 3.70e-22 96.3
MS.gene28547.t1 MTR_5g041380 48.387 93 46 1 46 136 97 189 3.74e-22 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28547.t1 AT5G60200 58.416 101 35 3 64 161 42 138 2.81e-33 124
MS.gene28547.t1 AT5G65590 53.846 104 42 2 35 138 8 105 3.63e-32 123
MS.gene28547.t1 AT1G07640 90.566 53 5 0 74 126 21 73 3.96e-32 122
MS.gene28547.t1 AT1G07640 90.566 53 5 0 74 126 77 129 7.28e-32 122
MS.gene28547.t1 AT1G07640 90.566 53 5 0 74 126 85 137 9.58e-32 122
MS.gene28547.t1 AT1G51700 63.750 80 23 1 52 125 5 84 9.84e-32 118
MS.gene28547.t1 AT2G28810 86.538 52 7 0 74 125 77 128 2.69e-31 120
MS.gene28547.t1 AT2G28810 86.538 52 7 0 74 125 94 145 3.66e-31 120
MS.gene28547.t1 AT5G60850 72.222 72 15 1 74 140 51 122 4.74e-31 119
MS.gene28547.t1 AT3G21270 88.679 53 6 0 74 126 29 81 8.25e-31 116
MS.gene28547.t1 AT2G37590 78.947 57 12 0 71 127 86 142 8.92e-31 119
MS.gene28547.t1 AT3G61850 54.255 94 43 0 34 127 34 127 1.59e-30 118
MS.gene28547.t1 AT3G61850 54.255 94 43 0 34 127 22 115 1.60e-30 117
MS.gene28547.t1 AT3G61850 54.255 94 43 0 34 127 22 115 1.60e-30 117
MS.gene28547.t1 AT3G61850 54.255 94 43 0 34 127 22 115 2.21e-30 118
MS.gene28547.t1 AT3G61850 54.255 94 43 0 34 127 34 127 2.93e-30 118
MS.gene28547.t1 AT3G45610 69.697 66 20 0 74 139 40 105 6.63e-30 115
MS.gene28547.t1 AT5G02460 83.333 54 9 0 74 127 95 148 1.12e-29 117
MS.gene28547.t1 AT3G55370 73.134 67 15 1 74 140 76 139 1.48e-29 116
MS.gene28547.t1 AT4G38000 67.105 76 24 1 53 127 20 95 1.55e-29 114
MS.gene28547.t1 AT3G55370 73.134 67 15 1 74 140 76 139 1.76e-29 116
MS.gene28547.t1 AT3G55370 70.000 70 18 1 71 140 118 184 2.10e-29 116
MS.gene28547.t1 AT3G50410 73.016 63 17 0 64 126 20 82 1.12e-28 112
MS.gene28547.t1 AT1G28310 51.042 96 47 0 34 129 11 106 1.18e-28 114
MS.gene28547.t1 AT1G28310 78.571 56 12 0 74 129 27 82 1.26e-28 113
MS.gene28547.t1 AT3G52440 84.615 52 8 0 76 127 27 78 2.36e-28 111
MS.gene28547.t1 AT2G28510 82.692 52 9 0 76 127 50 101 2.62e-28 112
MS.gene28547.t1 AT3G52440 84.615 52 8 0 76 127 46 97 3.20e-28 111
MS.gene28547.t1 AT5G66940 60.274 73 29 0 68 140 26 98 3.90e-28 109
MS.gene28547.t1 AT1G21340 55.056 89 39 1 76 163 40 128 1.01e-27 109
MS.gene28547.t1 AT4G00940 57.471 87 37 0 64 150 58 144 1.38e-27 110
MS.gene28547.t1 AT4G00940 57.471 87 37 0 64 150 58 144 1.38e-27 110
MS.gene28547.t1 AT4G00940 57.471 87 37 0 64 150 58 144 1.38e-27 110
MS.gene28547.t1 AT4G21050 65.625 64 22 0 72 135 22 85 3.75e-27 107
MS.gene28547.t1 AT2G46590 54.082 98 34 3 34 121 19 115 6.07e-27 109
MS.gene28547.t1 AT2G46590 54.082 98 34 3 34 121 31 127 7.23e-27 109
MS.gene28547.t1 AT4G24060 70.968 62 18 0 66 127 45 106 7.90e-27 108
MS.gene28547.t1 AT5G62940 80.000 50 10 0 75 124 74 123 3.24e-26 107
MS.gene28547.t1 AT1G29160 58.667 75 31 0 53 127 41 115 2.37e-25 100
MS.gene28547.t1 AT3G47500 74.545 55 14 0 74 128 110 164 6.62e-25 105
MS.gene28547.t1 AT5G39660 53.125 96 28 1 50 128 97 192 7.53e-25 105
MS.gene28547.t1 AT5G39660 53.125 96 28 1 50 128 97 192 7.53e-25 105
MS.gene28547.t1 AT5G39660 53.125 96 28 1 50 128 97 192 7.53e-25 105
MS.gene28547.t1 AT2G34140 55.263 76 34 0 52 127 36 111 7.87e-25 99.4
MS.gene28547.t1 AT1G64620 71.930 57 16 0 66 122 41 97 1.34e-24 103
MS.gene28547.t1 AT1G69570 55.844 77 34 0 64 140 122 198 1.38e-24 103
MS.gene28547.t1 AT1G26790 58.904 73 27 2 58 127 92 164 3.00e-24 102
MS.gene28547.t1 AT1G47655 55.696 79 32 1 44 122 2 77 3.32e-24 99.0
MS.gene28547.t1 AT5G62430 74.074 54 14 0 74 127 54 107 3.78e-24 100
MS.gene28547.t1 AT4G21040 73.585 53 14 0 76 128 27 79 3.96e-23 96.7
MS.gene28547.t1 AT4G21080 61.538 65 25 0 76 140 27 91 1.92e-22 95.1
MS.gene28547.t1 AT4G21030 53.704 54 25 0 72 125 19 72 1.75e-13 68.9

Find 100 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAAGTATATGAGAATTT+TGG 0.128090 8.4:+27596898 None:intergenic
TCATGATTGTTTCTCCAAAA+TGG 0.139233 8.4:+27596723 None:intergenic
TTCTTCTATATCCAACATTT+TGG 0.168024 8.4:-27596505 MS.gene28547:CDS
TCCTCCAACTCCTCCATTAA+TGG 0.195655 8.4:+27597361 None:intergenic
GCAGTTACTGGTGGTGAATT+AGG 0.209085 8.4:-27596474 MS.gene28547:CDS
GTTATACTTATTACTGATAT+TGG 0.212000 8.4:+27596385 None:intergenic
CATGATTAGGGTTAGGGTTT+TGG 0.228883 8.4:+27596856 None:intergenic
CCTTCTTCCTTAACATGATT+AGG 0.237816 8.4:+27596843 None:intergenic
CACCATTACCAAAGAGTTCT+TGG 0.238413 8.4:+27597423 None:intergenic
GTGGTGGTGGTTGTGGAAGA+TGG 0.243950 8.4:+27597299 None:intergenic
TTAGTTTCTGGTACAGTTCT+TGG 0.259576 8.4:+27596622 None:intergenic
CTTCTTCCTTAACATGATTA+GGG 0.273935 8.4:+27596844 None:intergenic
AGAAACTACTCTTCCATTAA+TGG 0.274062 8.4:-27597374 MS.gene28547:CDS
ACAGTTTCTTCAGAGTTTAA+TGG 0.275960 8.4:-27596996 MS.gene28547:CDS
GCAAGACATGCCGTCGTTAC+TGG 0.280270 8.4:-27597127 MS.gene28547:CDS
TTGGAAGATCAGACCAAGTT+AGG 0.282710 8.4:+27596430 None:intergenic
TTGGAGACAACTGCAGTTAC+TGG 0.305247 8.4:-27596486 MS.gene28547:CDS
TTGTTGATGACCTTAGTTTC+TGG 0.307492 8.4:+27596610 None:intergenic
GAAGAAGGCAATAACTTCAT+AGG 0.324773 8.4:-27596828 MS.gene28547:CDS
TGGTGCAGTGGTGGTGGTGG+TGG 0.332078 8.4:+27597280 None:intergenic
TTATACTTATTACTGATATT+GGG 0.334867 8.4:+27596386 None:intergenic
CAAAATGGTGGATTTGTACT+TGG 0.338045 8.4:-27596681 MS.gene28547:CDS
TCTTCACCAACAGTGTTACT+AGG 0.338562 8.4:-27596546 MS.gene28547:CDS
CCTTAACATGATTAGGGTTA+GGG 0.353211 8.4:+27596850 None:intergenic
CAAACTCAACAATTACAAAA+TGG 0.354663 8.4:-27596696 MS.gene28547:CDS
GACCAAGTTAGGGTTGGAAT+TGG 0.358551 8.4:+27596441 None:intergenic
TCCTTAACATGATTAGGGTT+AGG 0.359817 8.4:+27596849 None:intergenic
CATGATTGTTTCTCCAAAAT+GGG 0.372690 8.4:+27596724 None:intergenic
CAGAAAACCAACCACATCTA+AGG 0.374661 8.4:-27597226 MS.gene28547:CDS
TGGTGGTGGTTGTGGAAGAT+GGG 0.383636 8.4:+27597300 None:intergenic
TTTCCGACAGCCACCTCCTA+TGG 0.383757 8.4:+27597076 None:intergenic
CAGTTACTGGTGGTGAATTA+GGG 0.399545 8.4:-27596473 MS.gene28547:CDS
GACGGCATGTCTTGCAGAAA+TGG 0.400957 8.4:+27597135 None:intergenic
CAACCGTTGATTTCGTCAAA+TGG 0.418354 8.4:-27596777 MS.gene28547:CDS
GAAGAAGAAGGTTGTGTTGT+TGG 0.421244 8.4:+27597326 None:intergenic
CCCTAACCCTAATCATGTTA+AGG 0.421766 8.4:-27596850 MS.gene28547:CDS
CACCACAAAGTCAAATCCTA+TGG 0.426447 8.4:-27596929 MS.gene28547:CDS
TCCATTAATGGAGGAGTTGG+AGG 0.440892 8.4:-27597362 MS.gene28547:CDS
CCCCATAGGATTTGACTTTG+TGG 0.442522 8.4:+27596927 None:intergenic
CCTAATCATGTTAAGGAAGA+AGG 0.447706 8.4:-27596843 MS.gene28547:CDS
GGACAAAAGGAGGTGCTTTG+AGG 0.473653 8.4:-27597106 MS.gene28547:CDS
ATCCAATTCCAACCCTAACT+TGG 0.473948 8.4:-27596443 MS.gene28547:CDS
GGTGGTTGGTGCAGTGGTGG+TGG 0.475125 8.4:+27597274 None:intergenic
AAGATGATCCAAGAACTCTT+TGG 0.477513 8.4:-27597431 MS.gene28547:CDS
TGCCGTCGTTACTGGACAAA+AGG 0.477938 8.4:-27597119 MS.gene28547:CDS
AAAATGGTGGATTTGTACTT+GGG 0.478306 8.4:-27596680 MS.gene28547:CDS
ATTTCGTCAAATGGTTATGA+TGG 0.478918 8.4:-27596768 MS.gene28547:CDS
TCTTCCATTAATGGAGGAGT+TGG 0.482425 8.4:-27597365 MS.gene28547:CDS
GTGGTTGTGGTGACGGTGGT+TGG 0.490519 8.4:+27597260 None:intergenic
GGTTGGTGCAGTGGTGGTGG+TGG 0.494544 8.4:+27597277 None:intergenic
TGATGATGCAGTGGTGGTTG+TGG 0.494979 8.4:+27597247 None:intergenic
AAGAACTGTACCAGAAACTA+AGG 0.497513 8.4:-27596620 MS.gene28547:CDS
TGGAAGATCAGACCAAGTTA+GGG 0.500768 8.4:+27596431 None:intergenic
CTGGTGGTGAATTAGGGTAC+TGG 0.503929 8.4:-27596467 MS.gene28547:CDS
TTGGGTTTGAATTGCACCAT+TGG 0.506743 8.4:+27596404 None:intergenic
TCAACAACATCATCATCATC+AGG 0.508236 8.4:-27596594 MS.gene28547:CDS
GTGTTTGATGAGTCACATCT+TGG 0.511366 8.4:+27597200 None:intergenic
TGCAGTTGTCTCCAAAATGT+TGG 0.512043 8.4:+27596494 None:intergenic
ATCCAAGAACTCTTTGGTAA+TGG 0.516650 8.4:-27597425 MS.gene28547:CDS
TTGGACACCTTAGATGTGGT+TGG 0.518935 8.4:+27597219 None:intergenic
GACGGTGGTTGGTGCAGTGG+TGG 0.519460 8.4:+27597271 None:intergenic
TGCAGTGGTGGTTGTGGTGA+CGG 0.524860 8.4:+27597253 None:intergenic
TGAATTGCACCATTGGTAGT+TGG 0.526899 8.4:+27596411 None:intergenic
ACCACAAAGTCAAATCCTAT+GGG 0.531519 8.4:-27596928 MS.gene28547:CDS
AAGAAGGCAATAACTTCATA+GGG 0.534937 8.4:-27596827 MS.gene28547:CDS
TTCCGACAGCCACCTCCTAT+GGG 0.538444 8.4:+27597077 None:intergenic
GGAAGGTCAACAAGTGGTCA+AGG 0.546021 8.4:-27596975 MS.gene28547:CDS
GTTTCTTTCACTTGAAGTGA+GGG 0.555832 8.4:+27597391 None:intergenic
GGTGACGGTGGTTGGTGCAG+TGG 0.560684 8.4:+27597268 None:intergenic
TTGAGGAACGTTCCCATAGG+AGG 0.560915 8.4:-27597089 MS.gene28547:CDS
GAAGATGGGCGTGAAGAAGA+AGG 0.562429 8.4:+27597314 None:intergenic
CTTCACCAACAGTGTTACTA+GGG 0.563883 8.4:-27596545 MS.gene28547:CDS
AGGAACGTTCCCATAGGAGG+TGG 0.565496 8.4:-27597086 MS.gene28547:CDS
AGATCAGACCAAGTTAGGGT+TGG 0.572219 8.4:+27596435 None:intergenic
TCAAATGGTTATGATGGTGT+TGG 0.575378 8.4:-27596762 MS.gene28547:CDS
AGTTTCTTTCACTTGAAGTG+AGG 0.580149 8.4:+27597390 None:intergenic
AAATGGTGGATTTGTACTTG+GGG 0.583095 8.4:-27596679 MS.gene28547:CDS
TTCCCATAGGAGGTGGCTGT+CGG 0.589355 8.4:-27597079 MS.gene28547:CDS
ACTCAACAATTACAAAATGG+TGG 0.590751 8.4:-27596693 MS.gene28547:CDS
CATAGGATTTGACTTTGTGG+TGG 0.591845 8.4:+27596930 None:intergenic
TCTGATCTTCCAACTACCAA+TGG 0.595789 8.4:-27596420 MS.gene28547:CDS
TTTCTTCAGAGTTTAATGGA+AGG 0.603721 8.4:-27596992 MS.gene28547:CDS
AATTCATCATGTGAGAAACA+TGG 0.605341 8.4:+27596802 None:intergenic
ATTCATCATGTGAGAAACAT+GGG 0.610019 8.4:+27596803 None:intergenic
GAAGGTCAACAAGTGGTCAA+GGG 0.612566 8.4:-27596974 MS.gene28547:CDS
TAACCATTTGACGAAATCAA+CGG 0.616215 8.4:+27596774 None:intergenic
CGTCGTTACTGGACAAAAGG+AGG 0.618133 8.4:-27597116 MS.gene28547:CDS
GCTTTGAGGAACGTTCCCAT+AGG 0.619952 8.4:-27597092 MS.gene28547:CDS
CATCTTGGACACCTTAGATG+TGG 0.628308 8.4:+27597215 None:intergenic
AACTACTCTTCCATTAATGG+AGG 0.630490 8.4:-27597371 MS.gene28547:CDS
CATGTCTTGCAGAAATGGCG+AGG 0.648175 8.4:+27597140 None:intergenic
CATCACCATCATCAAAACAG+TGG 0.654071 8.4:-27596648 MS.gene28547:CDS
TTTAATGGAAGGTCAACAAG+TGG 0.666542 8.4:-27596981 MS.gene28547:CDS
ACGTTCCCTAGTAACACTGT+TGG 0.681503 8.4:+27596540 None:intergenic
AACAGTGTTACTAGGGAACG+TGG 0.681785 8.4:-27596538 MS.gene28547:CDS
AGAAATGGCGAGGCTGAGTG+AGG 0.701300 8.4:+27597150 None:intergenic
TATCAACGCCTCAAATAGCG+CGG 0.706731 8.4:-27597045 MS.gene28547:CDS
CCACAAAGTCAAATCCTATG+GGG 0.709378 8.4:-27596927 MS.gene28547:CDS
AGTGGTGGTTGTGGTGACGG+TGG 0.714798 8.4:+27597256 None:intergenic
GAGACAACTGCAGTTACTGG+TGG 0.722534 8.4:-27596483 MS.gene28547:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAAGTATATGAGAATTT+TGG + chr8.4:27596928-27596947 None:intergenic 20.0%
!!! AGAAAGTATATGAGAATTTT+GGG + chr8.4:27596927-27596946 None:intergenic 20.0%
! CAAACTCAACAATTACAAAA+TGG - chr8.4:27597127-27597146 MS.gene28547:CDS 25.0%
!! TTCTTCTATATCCAACATTT+TGG - chr8.4:27597318-27597337 MS.gene28547:CDS 25.0%
!!! GAAAGTATATGAGAATTTTG+GGG + chr8.4:27596926-27596945 None:intergenic 25.0%
AAGAAGGCAATAACTTCATA+GGG - chr8.4:27596996-27597015 MS.gene28547:CDS 30.0%
AATTCATCATGTGAGAAACA+TGG + chr8.4:27597024-27597043 None:intergenic 30.0%
ACAGTTTCTTCAGAGTTTAA+TGG - chr8.4:27596827-27596846 MS.gene28547:CDS 30.0%
ACTCAACAATTACAAAATGG+TGG - chr8.4:27597130-27597149 MS.gene28547:CDS 30.0%
AGAAACTACTCTTCCATTAA+TGG - chr8.4:27596449-27596468 MS.gene28547:CDS 30.0%
ATTCATCATGTGAGAAACAT+GGG + chr8.4:27597023-27597042 None:intergenic 30.0%
ATTTCGTCAAATGGTTATGA+TGG - chr8.4:27597055-27597074 MS.gene28547:CDS 30.0%
CTTCTTCCTTAACATGATTA+GGG + chr8.4:27596982-27597001 None:intergenic 30.0%
TAACCATTTGACGAAATCAA+CGG + chr8.4:27597052-27597071 None:intergenic 30.0%
TTTCTTCAGAGTTTAATGGA+AGG - chr8.4:27596831-27596850 MS.gene28547:CDS 30.0%
! ATGTTTTATGCAACCCATTT+TGG - chr8.4:27597086-27597105 MS.gene28547:CDS 30.0%
! CATGATTGTTTCTCCAAAAT+GGG + chr8.4:27597102-27597121 None:intergenic 30.0%
! TCATGATTGTTTCTCCAAAA+TGG + chr8.4:27597103-27597122 None:intergenic 30.0%
!! AAAATGGTGGATTTGTACTT+GGG - chr8.4:27597143-27597162 MS.gene28547:CDS 30.0%
AACTACTCTTCCATTAATGG+AGG - chr8.4:27596452-27596471 MS.gene28547:CDS 35.0%
AAGAACTGTACCAGAAACTA+AGG - chr8.4:27597203-27597222 MS.gene28547:CDS 35.0%
ACCACAAAGTCAAATCCTAT+GGG - chr8.4:27596895-27596914 MS.gene28547:CDS 35.0%
AGTTTCTTTCACTTGAAGTG+AGG + chr8.4:27596436-27596455 None:intergenic 35.0%
ATCCAAGAACTCTTTGGTAA+TGG - chr8.4:27596398-27596417 MS.gene28547:CDS 35.0%
CCTAATCATGTTAAGGAAGA+AGG - chr8.4:27596980-27596999 MS.gene28547:CDS 35.0%
CCTTAACATGATTAGGGTTA+GGG + chr8.4:27596976-27596995 None:intergenic 35.0%
CCTTCTTCCTTAACATGATT+AGG + chr8.4:27596983-27597002 None:intergenic 35.0%
GAAGAAGGCAATAACTTCAT+AGG - chr8.4:27596995-27597014 MS.gene28547:CDS 35.0%
GTTTCTTTCACTTGAAGTGA+GGG + chr8.4:27596435-27596454 None:intergenic 35.0%
TCAACAACATCATCATCATC+AGG - chr8.4:27597229-27597248 MS.gene28547:CDS 35.0%
TCCTTAACATGATTAGGGTT+AGG + chr8.4:27596977-27596996 None:intergenic 35.0%
TTTAATGGAAGGTCAACAAG+TGG - chr8.4:27596842-27596861 MS.gene28547:CDS 35.0%
! TCAAATGGTTATGATGGTGT+TGG - chr8.4:27597061-27597080 MS.gene28547:CDS 35.0%
!! AAATGGTGGATTTGTACTTG+GGG - chr8.4:27597144-27597163 MS.gene28547:CDS 35.0%
!! CAAAATGGTGGATTTGTACT+TGG - chr8.4:27597142-27597161 MS.gene28547:CDS 35.0%
!! TTAGTTTCTGGTACAGTTCT+TGG + chr8.4:27597204-27597223 None:intergenic 35.0%
!! TTGTTGATGACCTTAGTTTC+TGG + chr8.4:27597216-27597235 None:intergenic 35.0%
ATCCAATTCCAACCCTAACT+TGG - chr8.4:27597380-27597399 MS.gene28547:CDS 40.0%
CACCACAAAGTCAAATCCTA+TGG - chr8.4:27596894-27596913 MS.gene28547:CDS 40.0%
CACCATTACCAAAGAGTTCT+TGG + chr8.4:27596403-27596422 None:intergenic 40.0%
CAGAAAACCAACCACATCTA+AGG - chr8.4:27596597-27596616 MS.gene28547:CDS 40.0%
CATCACCATCATCAAAACAG+TGG - chr8.4:27597175-27597194 MS.gene28547:CDS 40.0%
CCACAAAGTCAAATCCTATG+GGG - chr8.4:27596896-27596915 MS.gene28547:CDS 40.0%
CCCTAACCCTAATCATGTTA+AGG - chr8.4:27596973-27596992 MS.gene28547:CDS 40.0%
GAAGAAGAAGGTTGTGTTGT+TGG + chr8.4:27596500-27596519 None:intergenic 40.0%
GTGTTTGATGAGTCACATCT+TGG + chr8.4:27596626-27596645 None:intergenic 40.0%
TCTGATCTTCCAACTACCAA+TGG - chr8.4:27597403-27597422 MS.gene28547:CDS 40.0%
TCTTCCATTAATGGAGGAGT+TGG - chr8.4:27596458-27596477 MS.gene28547:CDS 40.0%
TGCAGTTGTCTCCAAAATGT+TGG + chr8.4:27597332-27597351 None:intergenic 40.0%
TGGAAGATCAGACCAAGTTA+GGG + chr8.4:27597395-27597414 None:intergenic 40.0%
TTGGAAGATCAGACCAAGTT+AGG + chr8.4:27597396-27597415 None:intergenic 40.0%
TTGGGTTTGAATTGCACCAT+TGG + chr8.4:27597422-27597441 None:intergenic 40.0%
! CAGTTACTGGTGGTGAATTA+GGG - chr8.4:27597350-27597369 MS.gene28547:CDS 40.0%
! CATAGGATTTGACTTTGTGG+TGG + chr8.4:27596896-27596915 None:intergenic 40.0%
! CATGATTAGGGTTAGGGTTT+TGG + chr8.4:27596970-27596989 None:intergenic 40.0%
! CTTCACCAACAGTGTTACTA+GGG - chr8.4:27597278-27597297 MS.gene28547:CDS 40.0%
! TCTTCACCAACAGTGTTACT+AGG - chr8.4:27597277-27597296 MS.gene28547:CDS 40.0%
! TGAATTGCACCATTGGTAGT+TGG + chr8.4:27597415-27597434 None:intergenic 40.0%
!! CAACCGTTGATTTCGTCAAA+TGG - chr8.4:27597046-27597065 MS.gene28547:CDS 40.0%
!! TTGGTTTTCTGATGATGCAG+TGG + chr8.4:27596588-27596607 None:intergenic 40.0%
!!! GGATTCCACTGTTTTGATGA+TGG + chr8.4:27597183-27597202 None:intergenic 40.0%
ACGTTCCCTAGTAACACTGT+TGG + chr8.4:27597286-27597305 None:intergenic 45.0%
AGATCAGACCAAGTTAGGGT+TGG + chr8.4:27597391-27597410 None:intergenic 45.0%
CATCTTGGACACCTTAGATG+TGG + chr8.4:27596611-27596630 None:intergenic 45.0%
GAAGGTCAACAAGTGGTCAA+GGG - chr8.4:27596849-27596868 MS.gene28547:CDS 45.0%
GACCAAGTTAGGGTTGGAAT+TGG + chr8.4:27597385-27597404 None:intergenic 45.0%
TATCAACGCCTCAAATAGCG+CGG - chr8.4:27596778-27596797 MS.gene28547:CDS 45.0%
TCCATTAATGGAGGAGTTGG+AGG - chr8.4:27596461-27596480 MS.gene28547:CDS 45.0%
TCCTCCAACTCCTCCATTAA+TGG + chr8.4:27596465-27596484 None:intergenic 45.0%
TTGGACACCTTAGATGTGGT+TGG + chr8.4:27596607-27596626 None:intergenic 45.0%
TTGGAGACAACTGCAGTTAC+TGG - chr8.4:27597337-27597356 MS.gene28547:CDS 45.0%
! AACAGTGTTACTAGGGAACG+TGG - chr8.4:27597285-27597304 MS.gene28547:CDS 45.0%
! CCCCATAGGATTTGACTTTG+TGG + chr8.4:27596899-27596918 None:intergenic 45.0%
! CTCCTTTTGTCCAGTAACGA+CGG + chr8.4:27596709-27596728 None:intergenic 45.0%
! GCAGTTACTGGTGGTGAATT+AGG - chr8.4:27597349-27597368 MS.gene28547:CDS 45.0%
!! AATTTTGGGGAGAACCCCAT+AGG + chr8.4:27596913-27596932 None:intergenic 45.0%
!! GTTTTCTGATGATGCAGTGG+TGG + chr8.4:27596585-27596604 None:intergenic 45.0%
!! TGTTTTTGCCGCGCTATTTG+AGG + chr8.4:27596789-27596808 None:intergenic 45.0%
CATGTCTTGCAGAAATGGCG+AGG + chr8.4:27596686-27596705 None:intergenic 50.0%
CGTCGTTACTGGACAAAAGG+AGG - chr8.4:27596707-27596726 MS.gene28547:CDS 50.0%
GAAGATGGGCGTGAAGAAGA+AGG + chr8.4:27596512-27596531 None:intergenic 50.0%
GACGGCATGTCTTGCAGAAA+TGG + chr8.4:27596691-27596710 None:intergenic 50.0%
GAGACAACTGCAGTTACTGG+TGG - chr8.4:27597340-27597359 MS.gene28547:CDS 50.0%
GGAAGGTCAACAAGTGGTCA+AGG - chr8.4:27596848-27596867 MS.gene28547:CDS 50.0%
TGCCGTCGTTACTGGACAAA+AGG - chr8.4:27596704-27596723 MS.gene28547:CDS 50.0%
TTGAGGAACGTTCCCATAGG+AGG - chr8.4:27596734-27596753 MS.gene28547:CDS 50.0%
! CTGGTGGTGAATTAGGGTAC+TGG - chr8.4:27597356-27597375 MS.gene28547:CDS 50.0%
! TGATGATGCAGTGGTGGTTG+TGG + chr8.4:27596579-27596598 None:intergenic 50.0%
!! GCTTTGAGGAACGTTCCCAT+AGG - chr8.4:27596731-27596750 MS.gene28547:CDS 50.0%
!! GGACAAAAGGAGGTGCTTTG+AGG - chr8.4:27596717-27596736 MS.gene28547:CDS 50.0%
!! TGGTGGTGGTTGTGGAAGAT+GGG + chr8.4:27596526-27596545 None:intergenic 50.0%
AGAAATGGCGAGGCTGAGTG+AGG + chr8.4:27596676-27596695 None:intergenic 55.0%
AGGAACGTTCCCATAGGAGG+TGG - chr8.4:27596737-27596756 MS.gene28547:CDS 55.0%
GCAAGACATGCCGTCGTTAC+TGG - chr8.4:27596696-27596715 MS.gene28547:CDS 55.0%
TGCAGTGGTGGTTGTGGTGA+CGG + chr8.4:27596573-27596592 None:intergenic 55.0%
TTCCCATAGGAGGTGGCTGT+CGG - chr8.4:27596744-27596763 MS.gene28547:CDS 55.0%
TTCCGACAGCCACCTCCTAT+GGG + chr8.4:27596749-27596768 None:intergenic 55.0%
TTTCCGACAGCCACCTCCTA+TGG + chr8.4:27596750-27596769 None:intergenic 55.0%
!! GTGGTGGTGGTTGTGGAAGA+TGG + chr8.4:27596527-27596546 None:intergenic 55.0%
!! AGTGGTGGTTGTGGTGACGG+TGG + chr8.4:27596570-27596589 None:intergenic 60.0%
!! GTGGTTGTGGTGACGGTGGT+TGG + chr8.4:27596566-27596585 None:intergenic 60.0%
! GACGGTGGTTGGTGCAGTGG+TGG + chr8.4:27596555-27596574 None:intergenic 65.0%
! GGTGACGGTGGTTGGTGCAG+TGG + chr8.4:27596558-27596577 None:intergenic 65.0%
! GGTGGTTGGTGCAGTGGTGG+TGG + chr8.4:27596552-27596571 None:intergenic 65.0%
!! AGTGGTGGTGGTGGTGGTGG+TGG + chr8.4:27596540-27596559 None:intergenic 65.0%
!! GGTGGTGGTGGTGGTGGTTG+TGG + chr8.4:27596534-27596553 None:intergenic 65.0%
!! GGTTGGTGCAGTGGTGGTGG+TGG + chr8.4:27596549-27596568 None:intergenic 65.0%
!! TGCAGTGGTGGTGGTGGTGG+TGG + chr8.4:27596543-27596562 None:intergenic 65.0%
!! TGGTGCAGTGGTGGTGGTGG+TGG + chr8.4:27596546-27596565 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 27596395 27597450 27596395 ID=MS.gene28547
chr8.4 mRNA 27596395 27597450 27596395 ID=MS.gene28547.t1;Parent=MS.gene28547
chr8.4 exon 27596395 27597450 27596395 ID=MS.gene28547.t1.exon1;Parent=MS.gene28547.t1
chr8.4 CDS 27596395 27597450 27596395 ID=cds.MS.gene28547.t1;Parent=MS.gene28547.t1
Gene Sequence

>MS.gene28547

ATGATCCAAGAACTCTTTGGTAATGGTGCTAGTACTACCCTCACTTCAAGTGAAAGAAACTACTCTTCCATTAATGGAGGAGTTGGAGGAGTTTTACTTCAACCAACAACACAACCTTCTTCTTCACGCCCATCTTCCACAACCACCACCACCACCACCACCACTGCACCAACCACCGTCACCACAACCACCACTGCATCATCAGAAAACCAACCACATCTAAGGTGTCCAAGATGTGACTCATCAAACACAAAATTTTGTTACTACAACAACTACAACCTCACTCAGCCTCGCCATTTCTGCAAGACATGCCGTCGTTACTGGACAAAAGGAGGTGCTTTGAGGAACGTTCCCATAGGAGGTGGCTGTCGGAAAAACAAGAGTATCAACGCCTCAAATAGCGCGGCAAAAACAACTACAAACAAGATGAAAACAGTTTCTTCAGAGTTTAATGGAAGGTCAACAAGTGGTCAAGGGTTTGATCTCGAACACGTTGTTCCACCACAAAGTCAAATCCTATGGGGTTCTCCCCAAAATTCTCATATACTTTCTTTGCTAAGATCTAGTCAAAACCAAAACCCTAACCCTAATCATGTTAAGGAAGAAGGCAATAACTTCATAGGGTCCCATGTTTCTCACATGATGAATTCTCAACCGTTGATTTCGTCAAATGGTTATGATGGTGTTGGACATGTTTTATGCAACCCATTTTGGAGAAACAATCATGATAATCAAACTCAACAATTACAAAATGGTGGATTTGTACTTGGGGATCAACAACATCACCATCATCAAAACAGTGGAATCCAAGAACTGTACCAGAAACTAAGGTCATCAACAACATCATCATCATCAGGTAATAATTATCGCAGTGAGATTTCATCTTCACCAACAGTGTTACTAGGGAACGTGGCTTCAAATTCTTCTTCTATATCCAACATTTTGGAGACAACTGCAGTTACTGGTGGTGAATTAGGGTACTGGAATCCAATTCCAACCCTAACTTGGTCTGATCTTCCAACTACCAATGGTGCAATTCAAACCCAATATCAGTAA
Protein sequence

>MS.gene28547.t1

MIQELFGNGASTTLTSSERNYSSINGGVGGVLLQPTTQPSSSRPSSTTTTTTTTTAPTTVTTTTTASSENQPHLRCPRCDSSNTKFCYYNNYNLTQPRHFCKTCRRYWTKGGALRNVPIGGGCRKNKSINASNSAAKTTTNKMKTVSSEFNGRSTSGQGFDLEHVVPPQSQILWGSPQNSHILSLLRSSQNQNPNPNHVKEEGNNFIGSHVSHMMNSQPLISSNGYDGVGHVLCNPFWRNNHDNQTQQLQNGGFVLGDQQHHHHQNSGIQELYQKLRSSTTSSSSGNNYRSEISSSPTVLLGNVASNSSSISNILETTAVTGGELGYWNPIPTLTWSDLPTTNGAIQTQYQ